Repeat Femoral Arteriography in Hyperlipidemic Patients

1988 ◽  
Vol 29 (3) ◽  
pp. 303-309 ◽  
Author(s):  
U. Erikson ◽  
G. Helmius ◽  
A. Hemmingsson ◽  
G. Ruhn ◽  
A. G. Olsson
Keyword(s):  
Femoral Artery ◽  
Serum Lipid ◽  
Visual Analysis ◽  
Treated Group ◽  
Observer Variation ◽  
Lipid Lowering ◽  
Control Group ◽  
Initial Examination ◽  
Atherosclerotic Lesions ◽  
Femoral Arteriography

Femoral arteriography was performed in 62 patients with significant hyperlipidemia. Sixty were asymptomatic and two had intermittent claudication. The patients participated in a study aiming to demonstrate whether serum lipid lowering by drugs could influence the development of femoral artery atheromatous disease. Half of the patients were treated with fenofibrate and nicotinic acid and the other half served as a control group. At the first arteriography atherosclerotic lesions were found in 46 of the 62 patients (74%). Arteriography was repeated up to three times without complications. Visual analysis of angiograms revealed considerable inter-observer variation. An attempt was made to assess the angiograms by a computerized method which, however, still needs improvement and a computer designed for image analysis. Most patients had small or moderate atheromatous deposits in the femoral artery at the initial examination, in most cases showing no change during the study period of 18 months. Regression was found in five patients of the treated group, but in none of the control patients as judged by visual gradation (p<0.001).

scholarly journals Effect of lutein on lipid profile in hypercholesterolemic rats

2018 ◽  
Vol 7 (5) ◽  
pp. 859
Author(s):  
Uma Narayanamurthy ◽  
Anandhi M. ◽  
Manimekalai K.
Keyword(s):  
Fatty Infiltration ◽  
Treated Group ◽  
Lipid Lowering ◽  
Ldl Oxidation ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Chow Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Group I

Background: Hyperlipidemia or Dyslipidemia is the major cause of atherosclerosis1 and associated conditions. Low levels of high-density-lipoprotein cholesterol (HDL-C) are the major causes of increased atherogenic risk 1. Aggressive cholesterol reduction in patients with atherosclerotic disease is now the standard of care2. In addition to life style modification, patients with risk factors need lipid lowering drug therapy. The drugs available now do not reduce LDL oxidation, and oxidative stress associated with hyperlipidemia. In recent years, antioxidants have been subjected to epidemiological studies4 that have related their consumption to a reduction in the incidence of oxidative damage related diseases.Methods: Hypercholesterolemia was induced in rats by administration of high cholesterol diet for 30 days in standard rat chow diet. Rats were divided into four groups of six each. Group-I and II with intake of normal diet and High cholesterol diet respectively. Group III and IV are given high cholesterol diet along with Lutein 50mg/kg and Atorvastatin 5mg/kg orally once daily respectively. At the end of 30 days animals were subjected to overnight fasting. Blood samples were drawn by retro-orbital puncture for biochemical analysis. The animals were sacrificed after thiopentone injection and liver and aorta were dissected out and processed for histopathological study and biochemical analysis.Results: Lutein treated group showed even more significant reduction in TBARS levels than the normal control group and Atorvastatin treated group. The efficacy of Lutein in slowing down the atherosclerosis and fatty infiltration of liver is proved in this study.Conclusions: Hence the present study had shown significant hypolipidemic, antiatherogenic and antioxidant effect of Luetin in Hyperlipidemic rats. 

scholarly journals Effect of Ethanol Extract of Glycine Max (Soy Bean) on Serum Lipid Profile of Fat-Fed Hyperlipidemic Rats

2018 ◽  
Vol 10 (1) ◽  
pp. 21-26
Author(s):  
K Sharmin ◽  
R Ahmed ◽  
A Momtaz ◽  
SA Chowdhury ◽  
NA Maya ◽  
...  
Keyword(s):  
Glycine Max ◽  
Lipid Profile ◽  
Serum Lipid ◽  
Serum Lipid Profile ◽  
Lipid Lowering ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Experimentally Induced

Hypercholesterolemia is one of the important causes of cardiovascular disease related mortality and morbidity and recently it has become a significant issue in public health problem of developing countries. The purpose of the study was to see the effect of glycine max (soy bean) on serum lipid profile of experimentally induced hyperlipidemic rats. Hypercholesterolemia was induced by high cholesterol diet containing 1% cholesterol in olive oil. This study was done on 24 long Norwegian rats (Rattus norvegicus) weighing about 200-210 gram. For convenience, the study was divided into Experiment I and Experiment II. In experiment I, 12 rats were divided into two groups- Group A (n = 6, control group) fed on laboratory diet and Group B (n=6) fed on laboratory diet and soy bean extract for 35 days. In experiment II, the remaining 12 rats were divided into 2 groups, Group C (n=6) were fed on laboratory diet and high cholesterol diet (HCD) for 35 days and Group D (n=6) were fed laboratory diet and high cholesterol diet on initial 10 days and soy bean extract was added along with high cholesterol diet for next 25 days. Serum TC, TG, LDL-C and HDL-C were measured after 35 days. HCD increased TG, TC and LDL-C significantly in group C. Glycine max (soy bean) treated group showed that it decreased plasma TC, TG and LDL-C in experimentally induced hyperlipidemic rats but Glycine max (soy bean) did not show any significant lipid lowering effect in normolipidemic rats. The results of this experimental study indicate that Glycine max can act as a cholesterol lowering agent and thereby can improve cardiovascular functions.Bangladesh J Med Biochem 2017; 10(1): 21-26

HIGH AND VERY HIGH DENSITY LIPOPROTEINS ADMINISTRATION INHIBITS PROGRESSION OF EXPERIMENTAL ATHEROSCLEROSIS IN THE RABBIT

1987 ◽  
Author(s):  
J Badimon ◽  
L Bodimon ◽  
V Fuster
Keyword(s):  
Treated Group ◽  
Experimental Atherosclerosis ◽  
Epidemiologic Studies ◽  
Atherogenic Diet ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Atherosclerotic Lesions ◽  
Artery Disease ◽  
In Vivo Administration

Epidemiologic studies have shown an inverse relationship between HDL and coronary artery disease. We have previously demonstrated that in vivo administration of HDL-VHDL inhibits thedevelopment of atherosclerosis in cholesterol (cho)-fed rabbits. In the present study we have analyzed whether high levels of the physiological cholesterol acceptor, homologous HDL-VHDL,could inhibit the progression of established atherosclerotic lesions. Atherosclerosis was induced by feeding rabbits a 0.5% cho-rich diet for 2 months (140g/day). At that moment, a subgroup of animal (N=4) was sacrificed and their aortas showed 30 ± 8% of aortic atherosclerotic involvement. The remaining animals, kept on the same atherogenic diet, were randomly divided in two identical groups (N=7): a control and a treated group administered with 50 mg of HDL-VHDL a week for 4 weeks. HDL-VHDL fraction wasisolated from normal rabbit plasma byultracentrifugation at a density range of 1.063-1.25g/ml. The amount of HDL-VHDL administered was determined byits protein content according to Lowry"s technique. The 50mg of HDL-VHDL, measured as protein, contained1.4mg of total cholesterol, 1.43mg oftriglycerides and 0.6mg of phospholipids. At sacrifice, the treated group showed a marked decrease on the extent of aortic by fatty streaks (20 ± 6%X ± 1SE) as compared to(36% + 6) inthe control group (p < 0.05). Similar results were obtained in aortic wall lipid accumulation (See table, results expressed as X±1SEM; rag/gr dry aorta.)In conclusion, administration of HDL-VHDL induced a marked inhibition on the progression of atherosclerosis in cholesterol-fed rabbits.

Abstract 337: Berberine Attenuates Hyperlipidemia in Mice by a Novel Underlying Mechanism Involving Trib1

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Amar B Singh ◽  
Jingwen Liu
Keyword(s):  
Mrna Expression ◽  
Association Studies ◽  
Treated Group ◽  
Lipid Lowering ◽  
Control Group ◽  
Hypolipidemic Effect ◽  
Genome Wide Association Studies ◽  
Mrna Levels ◽  
Lipogenic Genes ◽  
Natural Lipid

Recent genome wide association studies have identified tribbles homolog 1 (Trib1) as a risk factor in Myocardial Infarction. Trib1 has been shown to regulate VLDL production and hepatic lipogenesis in mice. Liver-specific overexpression of Trib1 reduced hepatic VLDL production and knockdown of Trib1 elevated plasma TG and cholesterol due to the increased VLDL production. However, currently little is known about the regulation of endogenous Trib1 expression and the relationship with plasma lipid homeostasis. In this study, we demonstrate that treating hyperlipidemic mice with 200 mg/kg of berberine (BBR), a natural lipid lowering compounds for 2 weeks reduced plasma total cholesterol by 24% and VLDL-cholesterol by 73% as compared to control mice. Hepatic gene expression analysis revealed that LDLR mRNA levels were not changed by BBR treatment. However, Trib1 mRNA levels were 70% (p<0.01) higher in BBR-treated group than that of control group. The elevation of hepatic Trib1 mRNA levels was also observed in BBR-treated mice fed a regular diet. To further characterize the LDLR-independent mechanism responsible for plasma TG lowering, BBR (150 mg/kg) was orally administered to LDLR knockout mice and we observed a 47% (p<0.05) reduction of serum TG levels after 6-days of BBR treatment while serum TC levels were not reduced. Importantly, the reduction of serum TG in LDLR KO mice by BBR treatment was accompanied by approximately 2-fold (p<0.05) increase in hepatic Trib1 mRNA levels in BBR-treated group as compared to control group. Furthermore, upregulation of Trib1 mRNA expression by BBR was inversely associated with the expression of key lipogenic genes (ACC1, SCD1, C/EBPα, GPAT1, MLXIPL and DGAT1) and measures of lipogenesis. By using HepG2 in vitro model system, we show BBR treatment also significantly increases Trib1 mRNA expression and reduces mRNA and protein expression of lipogenic genes. Taken together, our studies demonstrate for the first time that hepatic expression of Trib1 is upregulated by the natural lipid lowering compound BBR and that Trib1 plays an important role in the hypolipidemic effect of BBR independent of LDLR.

Quantitation of Atherosclerosis in Femoral Arteriography with Ecg Gated Exposures

1988 ◽  
Vol 29 (3) ◽  
pp. 311-315 ◽  
Author(s):  
S. Nilsson ◽  
I. Berglund ◽  
H. Bylund ◽  
U. Erikson ◽  
G. Helmius ◽  
...  
Keyword(s):  
Femoral Artery ◽  
Ecg Gating ◽  
Atherosclerotic Lesions ◽  
Follow Up Studies ◽  
The Impact ◽  
Better Than ◽  
Femoral Arteriography

Using a densitometric method introduced in 1977 by Crawford et coll. (6) the volumes of segments of the femoral artery were calculated from two angiographic series in each of 13 patients. ECG gated exposures were used to minimise the error of the method. The reproducibility of the method was found to be better than in a previous study in which ECG gating was not used. The method may therefore be of use in long term angiographic follow-up studies of atherosclerotic lesions, although the impact of different investigation parameters on methodologic precision is still not fully known.

scholarly journals Blueberry effects on prediabetic nephropathy—a preclinical in vivo approach

2021 ◽  
Vol 31 (Supplement_2) ◽  
Author(s):  
Pedro Vieira ◽  
Sara Nunes ◽  
André Alves ◽  
Inês Preguiça ◽  
Artur Figueirinha ◽  
...  
Keyword(s):  
Gene Expression ◽  
Renal Function ◽  
Glucose Intolerance ◽  
Periodic Acid ◽  
Treated Group ◽  
Lipid Lowering ◽  
Control Group ◽  
Hepatic Lipid Accumulation ◽  
Male Wistar Rats

Abstract Background Prediabetic Nephropathy (PreDN) therapeutic arsenal remains scarce. Blueberries (BB) display insulin sensitizing, lipid-lowering, antioxidant and anti-inflammatory effects. Therefore, we aimed to assess the putative benefits arising from BB supplementation in experimental PreDN. Methods BB polyphenol (PP) content was assessed by HPLC/PDA/ESI-MSn. Twenty-four male Wistar rats were divided in three groups (n = 8) for 24 weeks: Control group (Standard chow, Sd), 45% High-Fat (HF) diet-induced PreDN group and BJ-treated group (HF+BJ) [25 g/kg BW per day from week 16 onwards]. Glycemic/insulinemic/lipidic profiles were examined. Renal function was assessed by serum/urinary measures of creatinine, albumin, uric acid and glucose. Kidney histological characterization was performed by Periodic Acid-Shiff and Gomori’s green trichrome staining. Relative gene expression (RT-PCR) was also evaluated (KIM-1, NLRP3). Values are means ± S.E.M (ANOVA/post-hoc tests). This work was approved by the ORBEA of iCBR-FMUC (9/2018). Results HPLC/PDA/ESI-MSn analysis revealed that the main PP’s present in BB are malvidin derivatives, quercetin-O-hexoside and caffeic acid. HF animals displayed increased BW, glucose intolerance, postprandial hyperinsulinemia, elevated circulating LDL-c, hepatic/renal lipidosis, mild impairments in renal function and histological lesions resembling the stages I/II of PreDN. Apart from ameliorating prediabetic glucose intolerance, BJ supplementation elicited hepatic lipid accumulation and increased gene expression of renal inflammasome and KIM-1 without major improvements on renal function and/or histopathology. Conclusions Even though BB was able to ameliorate prediabetic glucose intolerance, the BB dosage employed herein (25 g/kg BW) elicited deleterious effects in the kidney only perceived at a sub-clinical level.

scholarly journals Influence of technological processing on lipid-lowering activity of substances containing in porcine hearts and aortas

10.5219/1119 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 331-336
Author(s):  
Elena Kotenkova ◽  
Irina Chernukha
Keyword(s):  
Molecular Weight ◽  
Lipid Profile ◽  
Serum Lipid ◽  
Raw Material ◽  
Serum Lipid Profile ◽  
Lipid Lowering ◽  
Control Group ◽  
Standard Diet ◽  
Bioactive Substances ◽  
Wide Range

Edible by-products are a good source of nutrients and bioactive substances and could be used as functional ingredients or for biopeptides production natively contained in raw materials. A wide range of peptides are also formed during the enzymatic hydrolysis or food processing. The comparative results of the effectiveness of isolated certain protein and peptide fractions by ultrafiltration with the same natively presented in raw tissues, as well as the influence of heat treatment on biological activity of origin active substances are presented. The model of rat alimentary hyperlipidemia was developed by adding cholesterol and fat to the standard diet and vitamin D2 injection per os. Serum lipid profile was determined on automatic analyzer BioChem FC-360. Dynamic of changes in serum lipid profile was assessed as corresponding control group medium results in ratio to certain rat data. Two-dimensional electrophoresis (2DE) was performed according to the method of O’Farrell with isoelectric focusing in ampholine pH gradient (IEF-PAGE) with following identification by MALDI-TOF MS and MS/MS mass spectrometry. Consumption of native pig aorta and pig heart during 14th days led to normalization of lipid profile in serum of hyperlipidemic rats, while low molecular weight (LMUF, MW <5 kDa) and medium molecular weight (MMUF, MW = 5 – 30 kDa) ultrafiltrates of pig aorta extract did not strongly influenced on level of triglicerides and, on contrary, elevated high density cholesterol. Consumption of developed product by hyperlipidemic rats during 28th days did not lead to significant changes in serum lipid profile, while on 42nd day all ratios reached ones in group, which were treated with native raw material or isolated active fractions. The stability of developed product was confirmed by proteomic studies. Obtained results open prospects to modernization the technology, presumably use as a matrix dietary meat (e.g. poultry) with incorporated active identified components.

scholarly journals The Dynamics of Circulating Immune Complexes in Horses with Severe Equine Asthma

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1001
Author(s):  
Malwina Slowikowska ◽  
Joanna Bajzert ◽  
Julia Miller ◽  
Tadeusz Stefaniak ◽  
Artur Niedzwiedz
Keyword(s):  
Immune Complexes ◽  
Treated Group ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Initial Examination ◽  
Diagnostic Biomarkers ◽  
Non Invasive ◽  
Equine Asthma ◽  
Asthma Group ◽  
Asthma Episode

Non-invasive diagnostic biomarkers of equine asthma syndrome (EAS) from blood or urine are sought. The aim of this study was to assess the absorbance of circulating immune complexes (CICs) during the exacerbation, remission, and treatment of an asthma episode and assess the potential usefulness of CIC levels in the diagnosis and monitoring of the disease. The control group, asthma group, and treated asthma group each contained six horses. Following an initial examination and group classification, the horses were kept in a dusty environment for seven days and then moved to an asthma-friendly environment for three weeks (the treated group received injections of glucocorticoids). Blood was collected at baseline and on the 1st, 2nd, 3rd, 7th, 14th and 30th days. CIC was measured using the modified Haskova method. The time points did not show significant statistical differences. There was a significant decrease in CIC in the treated group, and a significant increase in CIC in the non-treated group on day 30. CIC did not support the EAS diagnosis, although it may help in monitoring patients. To the best of the authors’ knowledge, this is the first study to analyse the dynamics of CIC during environmental challenge, remission, and treatment.

Tryptophan-Niacin Metabolism in Rat with Puromycin Aminonucleoside-Induced Nephrosis

2006 ◽  
Vol 76 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Yukari Egashira ◽  
Shin Nagaki ◽  
Hiroo Sanada
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Enzyme Activities ◽  
Treated Group ◽  
Control Group ◽  
Puromycin Aminonucleoside ◽  
Low Level ◽  
Key Enzyme

We investigated the change of tryptophan-niacin metabolism in rats with puromycin aminonucleoside PAN-induced nephrosis, the mechanisms responsible for their change of urinary excretion of nicotinamide and its metabolites, and the role of the kidney in tryptophan-niacin conversion. PAN-treated rats were intraperitoneally injected once with a 1.0% (w/v) solution of PAN at a dose of 100 mg/kg body weight. The collection of 24-hour urine was conducted 8 days after PAN injection. Daily urinary excretion of nicotinamide and its metabolites, liver and blood NAD, and key enzyme activities of tryptophan-niacin metabolism were determined. In PAN-treated rats, the sum of urinary excretion of nicotinamide and its metabolites was significantly lower compared with controls. The kidneyα-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) activity in the PAN-treated group was significantly decreased by 50%, compared with the control group. Although kidney ACMSD activity was reduced, the conversion of tryptophan to niacin tended to be lower in the PAN-treated rats. A decrease in urinary excretion of niacin and the conversion of tryptophan to niacin in nephrotic rats may contribute to a low level of blood tryptophan. The role of kidney ACMSD activity may be minimal concerning tryptophan-niacin conversion under this experimental condition.

Haemostatic Clot Formation at Anastomosis of Synthetic Venous Graft in Defibrinogenated Dogs: A Scanning Electron Microscopic Study

1981 ◽  
Vol 45 (03) ◽  
pp. 276-281 ◽  
Author(s):  
S Ishimaru ◽  
E Berglin ◽  
H-A Hansson ◽  
A-C Teger-Nilsson ◽  
G William-Olsson
Keyword(s):  
Vena Cava ◽  
Treated Group ◽  
Control Group ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic Study ◽  
Fibrin Network ◽  
Scanning Electron Microscopic ◽  
Expanded Polytetrafluoroethylene Graft ◽  
Morphological Differences ◽  
Scanning Electron

SummaryA segment of the inferior vena cava was replaced by an expanded polytetrafluoroethylene graft in 13 dogs. Five of them served as a control group, while the other 8 were moderately or severely defibrinogenated with subcutaneous batroxobin. Plasma fibrinogen decreased to extremely low values throughout the experiment in the defibrinogenated dogs except in the moderately treated group in which it temporarily rose to 0.72-0.87 g/1 on the first postoperative day.Scanning electron microscopic observations of the haemostatic clot formed at the anastomoses of the graft revealed no significant morphological differences in platelet adhesion and/or aggregation between the three groups. These findings confirmed that platelets play a key role in primary haemostasis during defibrinogenation.The fibrin network was slightly diminished and only short fibrin filaments could be seen in the moderately and severely defibrinogenated groups respectively. These differences in composition of the clots are discussed in relation to their haemostatic capacity.

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