Preparation of Bioactive Hydroxyapatite on Pure Titanium

2009 ◽  
Vol 24 (1_suppl) ◽  
pp. 169-182 ◽  
Author(s):  
Wang Tianshi ◽  
Zhang Renji ◽  
Yan Yongnian
Keyword(s):  
Titanium Surface ◽  
Pure Titanium ◽  
Cell Counting ◽  
Cell Compatibility ◽  
Ha Coating ◽  
Micro Arc Oxidation ◽  
Surface Metal ◽  
Titanium Surfaces ◽  
Better Than ◽  
Complex Oxidation

In this study, a hydroxyapatite (HA) was coated on a pure titanium surface by means of a complex oxidation and hydrothermal treatment. First an anodic oxidation was done on the titanium plates, followed by micro-arc oxidation. The HA-coated specimens and pure titanium specimens were immersed in SLB for 1, 5, and 10 days, respectively, to study their electrochemical behavior. The corrosion currents of HA-coated specimens were less than pure titanium specimens. This indicated that HA coating prevented surface metal ions of the implant from dissolving, thereby, reducing the tissue toxicity. The cytotoxic effect on fibroblasts L929 cells was measured by cell counting after being seeded for 2, 4, 8, 12, and 24 h. The number of surface cell attachments on the HA-coated specimens was much greater than on pure titanium specimens. The morphology of the cells on the HA coating had normal shapes and spread well with some cells climbing onto surface pores while cells on the pure titanium were oval shaped. The results confirm that the cell compatibility on HA-coated ion titanium surfaces is much better than pure titanium.

Cellular Activity and Biomaterial's Surface Topography

2007 ◽  
Vol 539-543 ◽  
pp. 517-522 ◽  
Author(s):  
Barbara Nebe ◽  
Frank Luethen ◽  
Regina Lange ◽  
Ulrich Beck
Keyword(s):  
Protein Adsorption ◽  
Titanium Surface ◽  
Bone Cells ◽  
Pure Titanium ◽  
Active Surface ◽  
Complete Medium ◽  
Adapter Proteins ◽  
Cellular Activity ◽  
A Cell ◽  
Titanium Surfaces

The contact of a cell on the biomaterial’s surface is mediated by its adhesion components. The topography of titanium surfaces influences these adhesion components of osteoblasts, e.g. the integrins, the adapter proteins and the actin cytoskeleton. In our current experiments we were interested in why osteoblasts were strongly aligned to the grooves of a structured pure titanium surface (grade 2). The titanium was characterized by EIS to get insights in the electro-chemically active surface. We used MG-63 human bone cells, cultured in DMEM with 10% FCS at 37°C. For protein adsorption the titanium discs were incubated for 24h with complete medium containing soluble fibronectin at 37°C. Interestingly, only in the grooves cells adhered and were aligned and this is not dependent on the gravitation. The cell adhesion seems to depend on the protein adsorption of fibronectin which we could find to be adsorbed exclusively in the valleys. We speculate that there are local differences in electro-chemical characteristics of this structured titanium surface.

Preparation and Features of Nano-ZrO2 / HA Coating on Surface of Titanium Materials in Dental-Implant

2011 ◽  
Vol 295-297 ◽  
pp. 491-495
Author(s):  
Xiao Feng Pang ◽  
Yong Huang ◽  
Xian Yu Cao
Keyword(s):  
Composite Coating ◽  
Dental Implant ◽  
Titanium Surface ◽  
Double Layers ◽  
Ionic Form ◽  
Zirconia Coating ◽  
Coating Materials ◽  
Zirconium Oxides ◽  
Ha Coating ◽  
Titanium Surfaces

The nano-hydroxyapatite/zirconia coating of double layers on surface of titanium allay materials have been prepared using electrochemical method. The features and structures of the composite coating materials are studied and analyzed by the Scanning electron microscope (SEM) and EDAX measurement. The results show that nano-HA/ZrO2 are densely and uniformly deposited on the surface of titanium allays in ionic form, a stable gradient composite coating, in which the nano-zirconium oxides (ZrO2) are homogeneously distributed between HA and titanium surfaces, are obtained. The tensile strength experiment exhibits that the adhesion or combined strength of the coating with the titanium surface is higher and about 17GPa, which manifests the nano-HA/ ZrO2 coating is successfully combined on the surface of the titanium allay materials. The biological experiments represent that this material can be used in repairing of bone and medical dental- implant of teeth.

scholarly journals UV Light-Generated Superhydrophilicity of a Titanium Surface Enhances the Transfer, Diffusion and Adsorption of Osteogenic Factors from a Collagen Sponge

2021 ◽  
Vol 22 (13) ◽  
pp. 6811
Author(s):  
Masako Tabuchi ◽  
Kosuke Hamajima ◽  
Miyuki Tanaka ◽  
Takeo Sekiya ◽  
Makoto Hirota ◽  
...  
Keyword(s):  
Dental Implant ◽  
Titanium Surface ◽  
Uv Light ◽  
Active Material ◽  
Pure Titanium ◽  
Weight Ratio ◽  
Collagen Sponge ◽  
Uv Treatment ◽  
Titanium Surfaces ◽  
Osteogenic Factors

It is a significant challenge for a titanium implant, which is a bio-inert material, to recruit osteogenic factors, such as osteoblasts, proteins and blood effectively when these are contained in a biomaterial. The objective of this study was to examine the effect of ultraviolet (UV)-treatment of titanium on surface wettability and the recruitment of osteogenic factors when they are contained in an atelocollagen sponge. UV treatment of a dental implant made of commercially pure titanium was performed with UV-light for 12 min immediately prior to the experiments. Superhydrophilicity on dental implant surfaces was generated with UV-treatment. The collagen sponge containing blood, osteoblasts, or albumin was directly placed on the dental implant. Untreated implants absorbed only a little blood from the collagen sponge, while the UV-treated implants absorbed blood rapidly and allowed it to spread widely, almost over the entire implant surface. Blood coverage was 3.5 times greater for the UV-treated implants (p < 0.001). Only 6% of the osteoblasts transferred from the collagen sponge to the untreated implants, whereas 16% of the osteoblasts transferred to the UV-treated implants (p < 0.001). In addition, a weight ratio between transferred albumin on the implant and measured albumin adsorbed on the implant was 17.3% in untreated implants and 38.5% in UV-treated implants (p < 0.05). These results indicated that UV treatment converts a titanium surface into a superhydrophilic and bio-active material, which could recruite osteogenic factors even when they were contained in a collagen sponge. The transfer and subsequent diffusion and adsorption efficacy of UV-treated titanium surfaces could be useful for bone formation when titanium surfaces and osteogenic factors are intervened with a biomaterial.

scholarly journals Alterations to Titanium Surface Depending on the Fluorides and Abrasives in Toothpaste

Materials ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 51
Author(s):  
Takahiro Shuto ◽  
Yuichi Mine ◽  
Seicho Makihira ◽  
Hiroki Nikawa ◽  
Takanori Wachi ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Surface Texture ◽  
Titanium Surface ◽  
Testing Machine ◽  
Pure Titanium ◽  
Abrasive Particles ◽  
Black Spots ◽  
Color Differences ◽  
Titanium Surfaces ◽  
The Difference

Fluoride and abrasives in toothpastes may cause corrosion and deterioration of the titanium used for implants and other prostheses. The purpose of this study was to investigate how the presence or absence and types of fluoride and abrasives affected the titanium surface texture. Brushing with toothpastes was performed on pure-titanium discs using an abrasive testing machine. Unprocessed titanium discs without brushing were used as control samples. Surface roughness, color, and gloss of titanium were measured and the differences compared with the control were analyzed. Additionally, titanium surfaces and abrasives in toothpastes were observed using a scanning electron microscope to compare the surface texture of each sample. Some toothpastes (abrasive+) significantly increased the difference in surface roughness, color, and gloss, compared with ultrapure water. Toothpaste (fluoride+/abrasive+) that had many polygonal abrasive particles led to the largest color differences and exhibited notable scratches and a larger number of contaminant- or corrosion-like black spots. In contrast, brushing with toothpaste without fluoride or abrasives (fluoride−/abrasive−) caused little change to the titanium surface. These results suggest that both fluoride and abrasives in toothpaste used for brushing may be factors that affect surface texture and corrosion resistance of titanium.

scholarly journals Hydrothermal Synthesis of Zinc-Incorporated Nano-Cluster Structure on Titanium Surface to Promote Osteogenic Differentiation of Osteoblasts and hMSCs

2021 ◽  
Vol 8 ◽  
Author(s):  
Ze-hua Tang ◽  
Shan Su ◽  
Yao Liu ◽  
Wen-qing Zhu ◽  
Song-mei Zhang ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Titanium Surface ◽  
Hydrothermal Reaction ◽  
Simple Procedure ◽  
Cluster Structure ◽  
Pure Titanium ◽  
Titanium Surfaces ◽  
Potential Strategy ◽  
Nano Cluster

In this study, a novel modification strategy was established to synthesize a zinc-incorporated nano-cluster structure on titanium surface in a two-step hydrothermal reaction, and the osteogenic differentiation of osteoblasts and human bone marrow mesenchymal cells (hMSCs) was studied in the presence of this synthesized nanostructure. Analyses of the surface topography and elemental composition revealed that the zinc-containing cluster-like nanostructure was successfully prepared on the titanium surface. By altering the reaction time, three surface modifications were established. The three modified titanium surfaces had improved hydrophilicity and could continuously release zinc ions in a controlled manner. In vitro study displayed that three modified titanium surfaces, especially the samples prepared by reacting for 15 min, exhibited enhanced cell adhesion, proliferation, and osteogenic differentiation compared to the pure titanium surface. The study therefore conclude that the zinc-incorporated nano-cluster modification of titanium surface through a simple procedure can establish an enhanced osteogenic microenvironment and exhibit a potential strategy of titanium surface modification to accelerate the dental implant osseointegration.

Cell Compatibility of the Micro-Arc Oxidized Surface of Large Plastic Deformed TA2 In Vitro

2016 ◽  
Vol 852 ◽  
pp. 1220-1226
Author(s):  
Li Hua Zhu ◽  
Xiao Jing Xu ◽  
Ting Zhuo Chen ◽  
Xiao Ya Niu ◽  
Lin Xu
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Large Deformation ◽  
Structure Refinement ◽  
Pure Titanium ◽  
Cell Toxicity ◽  
Cell Compatibility ◽  
Proliferation Ability ◽  
Better Than

The cell toxicity, cell proliferation and cell adhesion behaviors on the micro-arc oxidized surface of the conventional and large plastic deformed pure titanium (TA2) were studied. The results show that all samples have no cell toxicity. The cell proliferation ability on the surface of the large plastic deformed pure titanium (TA2) is better than that of the conventional pure titanium (TA2). The numbers of cells adhered on micro-arc oxidized surface of the large plastic deformed of pure titanium (TA2) are more than that of conventional pure titanium (TA2). And the distribution of the cells and cell morphologies are better than that of the conventional pure titanium (TA2). Results all above show that structure refinement of large deformation of pure titanium (TA2) has more significant improvement on the cell compatibility of the micro-arc oxidized modified films.

Preparation and characterization of porous bioceramic layers on pure titanium surfaces obtained by micro-arc oxidation process

2017 ◽  
Vol 123 (3) ◽  
Author(s):  
Chi-Sheng Chien ◽  
Yu-Chien Hung ◽  
Ting-Fu Hong ◽  
Chung-Chun Wu ◽  
Tsung-Yuan Kuo ◽  
...  
Keyword(s):  
Oxidation Process ◽  
Pure Titanium ◽  
Porous Bioceramic ◽  
Micro Arc Oxidation ◽  
Titanium Surfaces

Studies on the Bioceramic Coating on Titamium Substrate Including Silicon and Calcium by Micro-Arc Oxidation and Osteoblast’s Adhesion and Proliferation on the Coating

2013 ◽  
Vol 683 ◽  
pp. 163-167 ◽  
Author(s):  
De Chao Li ◽  
Xin Liu ◽  
Lei Han ◽  
Jin Long Li ◽  
Su Cheng
Keyword(s):  
Pure Titanium ◽  
Porous Titanium ◽  
X Ray Diffraction ◽  
Energy Dispersion Spectrometer ◽  
Adhesion Ability ◽  
Micro Arc Oxidation ◽  
Oxidation Technology ◽  
Proliferation Ability ◽  
Main Component ◽  
Better Than

This study used micro-arc oxidation technology for pure titanium alloy surface modification, which 400μm titanium beads were sintered into porous titanium in vacuum conditions, and produced silicon and calcium coatings. Scanning electron microscope(SEM), X-ray diffraction (XRD) and Energy dispersion spectrometer(EDS) were employed to investigate the surface morphology and phase composition of the coatings. The results are listed as follows: the main component of porous titanium MAO coating is anatase TiO2,and the coating introduced silicon and calcium element successfully. The proliferation ability of osteoblasts on the oxidized and unoxidized surface was determined by CCK-8 method, The results indicated that both the proliferation and adhesion ability of osteoblast on the oxidized surface were better than the unoxidized surface.

scholarly journals Long-lasting renewable antibacterial porous polymeric coatings enable titanium biomaterials to prevent and treat peri-implant infection

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shuyi Wu ◽  
Jianmeng Xu ◽  
Leiyan Zou ◽  
Shulu Luo ◽  
Run Yao ◽  
...  
Keyword(s):  
Dental Implant ◽  
Pathogenic Bacteria ◽  
Titanium Surface ◽  
Polymeric Coating ◽  
Antibacterial Efficacy ◽  
Polymeric Coatings ◽  
Implant Infection ◽  
Titanium Surfaces

AbstractPeri-implant infection is one of the biggest threats to the success of dental implant. Existing coatings on titanium surfaces exhibit rapid decrease in antibacterial efficacy, which is difficult to promisingly prevent peri-implant infection. Herein, we report an N-halamine polymeric coating on titanium surface that simultaneously has long-lasting renewable antibacterial efficacy with good stability and biocompatibility. Our coating is powerfully biocidal against both main pathogenic bacteria of peri-implant infection and complex bacteria from peri-implantitis patients. More importantly, its antibacterial efficacy can persist for a long term (e.g., 12~16 weeks) in vitro, in animal model, and even in human oral cavity, which generally covers the whole formation process of osseointegrated interface. Furthermore, after consumption, it can regain its antibacterial ability by facile rechlorination, highlighting a valuable concept of renewable antibacterial coating in dental implant. These findings indicate an appealing application prospect for prevention and treatment of peri-implant infection.

scholarly journals SEM and EDS Characterization of Porous Coatings Obtained On Titaniumby Plasma Electrolytic Oxidation in Electrolyte Containing Concentrated Phosphoric Acid with Zinc Nitrate

2017 ◽  
Vol 17 (2) ◽  
pp. 41-54 ◽  
Author(s):  
K. Rokosz ◽  
T. Hryniewicz ◽  
K. Pietrzak ◽  
W. Malorny
Keyword(s):  
Phosphoric Acid ◽  
Plasma Electrolytic Oxidation ◽  
Pure Titanium ◽  
Zinc Nitrate ◽  
Porous Coatings ◽  
Electrolytic Oxidation ◽  
Micro Arc Oxidation ◽  
Plasma Electrolytic ◽  
Peo Coatings

AbstractThe SEM and EDS results of porous coatings formed on pure titanium by Plasma Electrolytic Oxidation (Micro Arc Oxidation) under DC regime of voltage in the electrolytes containing of 500 g zinc nitrate Zn(NO3)2·6H2O in 1000 mL of concentrated phosphoric acid H3PO4at three voltages, i.e. 450 V, 550 V, 650 V for 3 minutes, are presented. The PEO coatings with pores, which have different shapes and the diameters, consist mainly of phosphorus, titanium and zinc. The maximum of zinc-to-phosphorus (Zn/P) ratio was found for treatment at 650 V and it equals 0.43 (wt%) | 0.20 (at%), while the minimum of that coefficient was recorded for the voltage of 450 V and equaling 0.26 (wt%) | 0.12 (at%). Performed studies have shown a possible way to form the porous coatings enriched with zinc by Plasma Electrolytic Oxidation in electrolyte containing concentrated phosphoric acid H3PO4with zinc nitrate Zn(NO3)2·6H2O.

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