Urine protein, urine protein to creatinine ratio and N-acetyl-β-D-glucosaminidase index in cats with idiopathic cystitis vs healthy control cats

2016 ◽  
Vol 19 (8) ◽  
pp. 869-875 ◽  
Author(s):  
Isadee Panboon ◽  
Sariya Asawakarn ◽  
Rosama Pusoonthornthum
Keyword(s):  
Protein Concentration ◽  
Colorimetric Method ◽  
Creatinine Ratio ◽  
Urine Protein ◽  
Blood Samples ◽  
Clinically Normal ◽  
Urine Creatinine ◽  
Healthy Control ◽  
The Mean

Objectives The objective was to compare urine protein, urine protein to creatinine ratio (UPC) and N-acetyl-β-D-glucosaminidase (NAG) index between cats with idiopathic cystitis and clinically normal cats. Methods Urine and blood samples were collected from 19 clinically normal cats and 19 cats with idiopathic cystitis without azotaemia at the time of first presentation. Urine protein, urine creatinine and UPC were measured. Additionally, the urinary NAG concentration was measured using the colorimetric method, and the NAG index was calculated by dividing the urinary NAG concentration by the urine creatinine ratio. Results Urine protein concentration (mean ± SEM) was four times higher in cats with idiopathic cystitis (218.29 ± 58.95) than in clinically normal cats (56.13 ± 9.95) (P <0.05). The UPC (mean ± SEM) of cats with idiopathic cystitis (0.70 ± 0.19) was also five times higher than that of clinically normal cats (0.14 ± 0.02) (P <0.05). The mean ± SEM NAG index in cats with idiopathic cystitis (4.79 ± 1.53 U/g) was two times higher than that in clinically normal cats (2.14 ± 0.48 U/g). The log UPC was positively correlated with the log NAG index in cats with idiopathic cystitis at moderate levels (r2 = 0.512; P <0.05). Conclusions and relevance Cats with idiopathic cystitis had increased amounts of urine protein and an increased UPC. Further study is needed to address the role of urinary NAG and its relationship with glycosaminoglycan levels in cats with idiopathic cystitis.

scholarly journals Study of the Immunological Status of Iraqi Vitiligo Patients

2016 ◽  
Vol 13 (3) ◽  
pp. 454-462
Author(s):  
Baghdad Science Journal
Keyword(s):  
Potential Role ◽  
Immunoglobulin E ◽  
Ultraviolet B ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Serum ◽  
Healthy Control ◽  
The Mean ◽  
Age Range ◽  
Apparently Healthy

Vitiligo is an acquired idiopathic skin disorder characterized by depigmented macules due to loss of cutaneous melanocytes. A potential role of the immune dysfunction has been suggested in vitiligo, so to test this hypothesis, certain cytokines (IL-17A and TNF-?) and immunoglobulins (IgM, IgG, IgA and total IgE) were investigated in all participants. The study included: 60 patients with age range between (6-55) year; 30(11 males and 19 females) were untreated and 30(12 males and 18 females) were treated with Narrow Band Ultraviolet-B (NB-UVB) and 30 (14 males and 16 females) apparently healthy control. Serum was separated and cytokines (IL-17A and TNF-?) and total immunoglobulin E (IgE) were detected by using Enzyme Linked Immunosorbent Assay (ELISA); while immunoglobulins (IgM, IgG and IgA) were detected by using Single Radial Immunodiffusion (SRID) method. The results showed that the mean levels of serum IL-17A and TNF-? in both untreated and NB-UVB treated vitiligo patients were increased significantly (p ? 0.05) as compared with healthy control. The mean levels of serum IgG and IgA in untreated vitiligo patients showed non significant decreased (P

scholarly journals THE USE OF HYDROXYETHYL STARCH 200/0,5 AS PLASMA SUBTITUTES IS SAFE IN HYPOVOLEMIC PATIENTS AS INDICATED IN CHANGES OF N-ACETYL--GLUCOSAMINIDASE AND CREATININ SERUM PARAMETERS

2016 ◽  
Vol 51 (4) ◽  
pp. 228
Author(s):  
Dewi Wara Shinta ◽  
Junaidi Khotib ◽  
Eddy Rahardjo ◽  
Mahardian Rahmadi ◽  
Budi Suprapti
Keyword(s):  
Hydroxyethyl Starch ◽  
Elective Surgery ◽  
Prior History ◽  
Creatinine Ratio ◽  
Urine Creatinine ◽  
Tissue Edema ◽  
History Of ◽  
The Mean ◽  
Baseline Characteristics ◽  
Two Parameters

Hydroxyethyl Starch (HES) is a compound that improves intravascular volume effectively and rapidly without causing tissue edema. However, HES also has renal safety profile which is still being debated. Based on clinical experience in Dr. Soetomo Hospital, the frequency of acute renal failure following HES 200/0.5 administration at a dose of less than 20 ml/kg (maximum dose) is very rare. The purpose of this study was to evaluate the effect of HES 200/0.5 at a dose of less than 20 ml/kg in patients undergoing surgery. N-acetyl-b-D-Glucosaminidase (NAG) per urine creatinine ratio and creatinine serum were used as main parameter to assess renal injury. This research was observational and prospective design in patients undergoing elective surgery at Gedung Bedah Pusat Terpadu, Dr. Soetomo Hospital, who requiring resuscitation therapy with HES 200/0.5 and met the inclusion and exclusion criteria. NAG was measured prior to surgery and 12 hours after administration of fluid therapy, while creatinine serum was observed before surgery and 48 hours after resuscitation. This study was conducted for three months, and obtained 50 subjects divided into 2 groups, crystalloid group and HES 200/0.5 group. Demographic and baseline characteristics did not differ between groups, except the total bleeding volume. Total bleeding in HES 200/0.5group was higher than crystalloid group (p <0.0001). The mean volume of fluid received in HES 200/0.5 group was 2042.0 ± 673.9 mL, higher when compared with that of crystalloid group (910.0 ± 592.0 ml). Doses of HES 200/0.5 received was 8.31 ± 4.86 ml/kg. Measurement of the of NAG/creatinine ratio and creatinine serum showed significant increase in both groups, but still within the normal range. In addition, the value of these two parameters did not differ between groups. In conclusion, HES 200/0.5 in a dose of less than 20 ml/kg is safe to use in patients who suffered from hypovolemic hemorrhage, without prior history of renal impairment.

scholarly journals Role of Urinary Calcium and Creatinine Ratio in Assessing Bone Resorption in Lepromatous Leprosy

2021 ◽  
Vol 48 (3) ◽  
pp. 28-33
Author(s):  
Saima Akhter ◽  
Md Qamrul Hassan Jaigirdar ◽  
Md Mostaque Mahmud ◽  
Shawana Haque ◽  
Rahat Bin Habib
Keyword(s):  
Bone Resorption ◽  
Lepromatous Leprosy ◽  
Urinary Calcium ◽  
Control Group ◽  
X Rays ◽  
Creatinine Ratio ◽  
Leprosy Patients ◽  
Healthy Control ◽  
Hands And Feet

Bony changes in lepromatous leprosy are one of the causes of deformity and disability. Fasting calcium and creatinine ratio in urine is used as a bone resorption marker in a number of diseases such as hyperthyroidism, osteoporosis, multiple myeloma, paget’s disease and sarcodosis. In lepromatous leprosy assessment of bone resorption might be done with that marker. To assess the role of fasting urinary calcium and creatinine ratio as a marker of bone resorption in patients with lepromatous leprosy. A case control study was conducted on 28 patients diagnosed as lepromatous leprosy and 28 age-matched healthy control. The participants who fulfilled all inclusion and exclusion criteria were studied by measuring fasting urinary calcium and creatinine level as well as observing X-rays of both hands and feet of affected individuals. The mean age of cases 38.1±14.2 years and 38.9±12.9 years was in control group. Male - female ratio was 3.6: 1. It was observed that 10.7% leprosy patients showed urinary Ca/Cr ratio >0.20 (0.13±0.12) and 10.7% healthy control showed urinary Ca/Cr ratio ratio>0.20 (mean ±SD 0.11±0.7). the difference was not statistically significant (p>0.05). X-ray finding was positive in 14.3% leprosy patients and none of the control group. That difference was not significant statistically (p>0.05). there was no relation between raised urinary Ca/Cr ratio and positive findings of bone resorption on x-rays among the leprosy cases. Bangladesh Med J. 2019 Sep; 48 (3): 28-33

scholarly journals Pathophysiological role of Atg5 in human ulcerative colitis

2020 ◽  
Vol 18 (4) ◽  
pp. 421-429
Author(s):  
Razieh Ardali ◽  
Nasrin Kazemipour ◽  
Saeed Nazifi ◽  
Kamran Bagheri Lankarani ◽  
Iman Razeghian Jahromi ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Significant Difference ◽  
Colon Biopsy ◽  
Pathophysiological Role ◽  
Healthy Control ◽  
Inflammatory Bowel ◽  
Insight Into

Background/Aims: Ulcerative colitis (UC), along with Crohn’s disease, is one of the main types of inflammatory bowel disease (IBD). On the other hand, deregulated autophagy is involved in many chronic diseases, including IBD. In this study, we aimed to investigate the role of Atg5 and microRNA-181a (miR-181a) in the pathophysiology of UC. Methods: Colon biopsy, stool, and blood samples of 6 men and 9 women were confirmed for UC. Also, 13 men and 17 women were selected as healthy control (HC). Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to measure the Atg-5 content of the colon biopsies. Besides, the serum and stool levels of Atg5 were measured using ELISA. Moreover, the total RNA of blood cells was extracted and evaluated for the expression of miR-181a.Results: We found 1.2 ng/mL versus 0.46 ng/mL, 0.34 ng/mL versus 0.24 ng/mL, and 0.082 ng/mL versus 0.062 ng/mL of Atg5 in stool, intestinal tissue, and serum of UC and HCs, respectively. There was no significant difference in the expression of miR-181a in the blood samples of UC and HCs. Immunohistochemistry showed high positivity without any significant difference between the 2 groups in the quantitative analysis.Conclusions: The significant difference observed between the stool Atg5 content of the HCs and UC patients may provide new insight into using this protein as a diagnostic biomarker, however, considering the small size of our studied population further studies are needed.

Importance of the FcγRIIa-Arg/His-131 Polymorphism in Heparin-induced Thrombocytopenia Diagnosis

1998 ◽  
Vol 79 (03) ◽  
pp. 523-528 ◽  
Author(s):  
Raphaël Saffroy ◽  
Dominique Lasne ◽  
Gilles Chatellier ◽  
Martine Aiach ◽  
Francine Rendu ◽  
...  
Keyword(s):  
Serotonin Release ◽  
Positive Answer ◽  
Control Group ◽  
Normal Donor ◽  
Heparin Induced Thrombocytopenia ◽  
Weak Association ◽  
Healthy Control ◽  
Release Assay ◽  
The Mean

SummaryHeparin-induced thrombocytopenia (HIT) involves heparin-dependent antibodies which induce platelet activation. In the present study, we searched for a relationship between the polymorphism of the Fc receptor (FcγRIIa) and the development of HIT. In this purpose, all the donors were genotyped for their FcγRIIA and HIT patients were selected on the basis of at least one positive answer by 14C-serotonin release assay (SRA). The frequency distribution of the FcγRIIa polymorphism in the HIT patient group was similar to that observed in the healthy control group. Moreover, a statistical analysis taking into account our results and those of 3 previously published studies, suggested at most only a weak association between HIT and the FcγRIIa-131 polymorphism.Laboratory tests used to diagnose HIT rely on the activation of normal donor platelets but fail to detect every HIT positive patient. We determined the role of FcγRIIa-131 polymorphism on the reactivity of control platelets to HIT plasmas. When control platelet FcγRIIa-131 was of Arg/Arg form, only 47% of the HIT plasmas were positive by SRA, compared to 81% and 74% for His/His or His/Arg forms, respectively. We also compared the level of anti PF4/heparin antibodies in the HIT plasmas with the response obtained by SRA. The mean anti PF4/heparin antibodies level in HIT plasma was significantly lower in negative SRA than in positive tests when using control platelets from FcγRIIa-Arg/Arg131 and heterozygous donors. Thus, the variability of control platelets to respond to HIT plasmas in the SRA test is related to both the FcγRIIa-131 polymorphism, and to the amount of anti PF4/heparin antibodies.

scholarly journals P0393NEPHROTIC SYNDROME IN POST HEMATOPOIETIC STEM CELL TRANSPLANTATION PATIENTS-20 YEARS' EXPERIENCE IN A SINGLE CENTER

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Myung Ah Ha ◽  
Chul-Woo Yang ◽  
Yong-Soo Kim ◽  
Cheol Whee Park
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Myeloid Leukemia ◽  
Kidney Biopsy ◽  
Cytotoxic Agents ◽  
Creatinine Ratio ◽  
Urine Protein ◽  
Hematopoietic Stem ◽  
The Mean

Abstract Background and Aims Nephrotic syndrome (NS) is an extremely rare complication of hematopoietic stem cell transplantation(HCT) that usually occurs in association with chronic graft-versus-host disease. The etiology and pathogenesis of NS in HCT recipients remain unclear. There are only a few case reports and case series of NS following HCT that have been published. Method We reviewed the records of kidney biopsy in Seoul St. Mary’s Hospital between January, 2008 and January, 2019, and identified 32 kidney biopsy specimens from patients obtained after HCT. All patients underwent kidney biopsy for proteinuria or increased creatinine. Patient demographic data and laboratory data were collected for analysis. Responses to treatment were categorized as complete remission (CR), partial remission (PR), or non-response. The CR and PR were defined as a reduction of urine protein/creatinine ratio to &lt;0.3g/day and urine protein/creatinine ratio between 0.31g/day and 3.4g/day, respectively. Results Of the 32 cases, 21 were male and 11 were female patients. The mean patient age was 43.7(range: 15-68). Average follow-up period was 46.6 months (range: 2-107 months). The mean duration between HCT and renal biopsy was 33.2 months (range: 6–206 months). The underlying diseases resulting in the need for HCT were acute myeloid leukemia, 10 patients; acute lymphoid leukemia, 9 patients; aplastic anemia, 4 patients; myelodysplastic syndrome, 3 patients; multiple myeloma, 3 patients; chronic myeloid leukemia, 1 patient; amyloidosis, 1 patient; and blastic plasmacytoid dendritic cell neoplasm, 1 patient. The most common histopathological finding was membranous glomerulonephritis (N=20). Other glomerular diseases identified were thrombotic microangiopathy, amyloidosis, C1q nephropathy, focal segmental glomerulosclerosis and diabetic nephropathy. During the follow-up period, 25 patients were treated with steroids or cytotoxic agents.18 patients were treated with angiotensin II receptor antagonist. There was no statistically significant difference between the groups in terms of ANOVA of the decrease in proteinuria according to steroids or cytotoxic agents treatment (p = 0.075) and ARB treatment. (p = 0.156) Conclusion Based on our result, more case will require discussion of treatment options for NS following HCT.

scholarly journals Assessment of Proteinuria in Nephrotic Syndrome in Children by Using Spot Urinary Protein/Creatinine Ratio

2018 ◽  
Vol 42 (3) ◽  
pp. 108-111
Author(s):  
Delowar Hossain ◽  
Zahiruddin ◽  
Monimul Hoque
Keyword(s):  
Nephrotic Syndrome ◽  
Total Protein ◽  
Mean Value ◽  
Urinary Protein ◽  
Urine Collection ◽  
Creatinine Ratio ◽  
Urine Protein ◽  
Spot Urine ◽  
Protein Excretion ◽  
The Mean

Background: Quantification of proteinuria is usually predicted upon 24-hour urine collection. Multiple factors influence urine collection and the rate of protein and creatinine excretion. A spot urine protein-creatinine (P-C) ratio has been shown over the years to be a reliable alternative to the 24-hour collection for detection and follow up of proteinuria. The objective of the study was to evaluate the accuracy of urine protein creatinine ratio (UP/UC) in a spot sample for quantitative measurement of proteinuria in comparison with 24 hours urinary protein excretion in children of nephrotic syndrome having normal Glomerular Filtration Rate (GFR). Methodology: This was a prospective study conducted in the department of paediatrics, Sir Salimullah Medical College & Mitford Hospital Dhaka over a period of six months from January 2003. Fifty cases of Nephrotic syndrome were included who were on initial attack and relapse cases noted down into the proforma with respect to history, examination and investigation. All the patients were advised regarding 24 hours urine collection. They were asked to give a 24 hours urine sample starting at 9.00 am for total protein excretion rate. A spot urine sample was obtained and urine protein/creatinine ratio was calculated. The data was analyzed by linear regression and by calculating the correlation coefficient between urinary protein/ creatinine ratio and 24-hour urinary protein. Results: Sample size was fifty. Urine total protein in a timed 24-hour sample of nephrotic syndrome patients was in the range of 300-3150mg/m2/hour with the mean value of 1725 mg/m2/hour. While as U(Pr/Cr) ratio ranged from 3.1-27.5 with the mean value of 15.2. A significant correlation was found between timed 24-hour urinary protein and UP/UC ratio (r=0.622, p=<.001.) Conclusions: Spot urine protein-creatinine ratio is highly reliable and rapid test for quantification of proteinuria in children with nephrotic syndrome. Bangladesh J Child Health 2018; VOL 42 (3) :108-111

Effect of blood contamination on results of dipstick evaluation and urine protein-to-urine creatinine ratio for urine samples from dogs and cats

2018 ◽  
Vol 79 (5) ◽  
pp. 525-531 ◽  
Author(s):  
Aida I. Vientós-Plotts ◽  
Ellen N. Behrend ◽  
Elizabeth G. Welles ◽  
Dennis J. Chew ◽  
Philippe R. Gaillard ◽  
...  
Keyword(s):  
Urine Samples ◽  
Creatinine Ratio ◽  
Urine Protein ◽  
Blood Contamination ◽  
Urine Creatinine

scholarly journals Minimal Change Disease and IgA Deposition: Separate Entities or Common Pathophysiology?

2013 ◽  
Vol 2013 ◽  
pp. 1-3
Author(s):  
Brandon S. Oberweis ◽  
Aditya Mattoo ◽  
Ming Wu ◽  
David S. Goldfarb
Keyword(s):  
Iga Nephropathy ◽  
Minimal Change Disease ◽  
Minimal Change ◽  
Dramatic Improvement ◽  
Creatinine Ratio ◽  
Urine Protein ◽  
Common Cause ◽  
History Of ◽  
Foot Process

Introduction. Minimal Change Disease (MCD) is the most common cause of nephrotic syndrome in children, while IgA nephropathy is the most common cause of glomerulonephritis worldwide. MCD is responsive to glucocorticoids, while the role of steroids in IgA nephropathy remains unclear. We describe a case of two distinct clinical and pathological findings, raising the question of whether MCD and IgA nephropathy are separate entities or if there is a common pathophysiology.Case Report. A 19-year old man with no medical history presented to the Emergency Department with a 20-day history of anasarca and frothy urine, BUN 68 mg/dL, Cr 2.3 mg/dL, urinalysis 3+ RBCs, 3+ protein, and urine protein : creatinine ratio 6.4. Renal biopsy revealed hypertrophic podocytes on light microscopy, podocyte foot process effacement on electron microscopy, and immunofluorescent mesangial staining for IgA. The patient was started on prednisone and exhibited dramatic improvement.Discussion. MCD typically has an overwhelming improvement with glucocorticoids, while the resolution of IgA nephropathy is rare. Our patient presented with MCD with the uncharacteristic finding of hematuria. Given the improvement with glucocorticoids, we raise the question of whether there is a shared pathophysiologic component of these two distinct clinical diseases that represents a clinical variant.

scholarly journals A Preliminary estimation of baseline urinary hydroxyproline/creatinine levels in a study population of healthy Nigerians

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Chidinma Ifechi Onwuka ◽  
Chidozie Onwuka ◽  
Chibuzor Uguru
Keyword(s):  
Colorimetric Method ◽  
Early Morning ◽  
Bivariate Analysis ◽  
Biological Processes ◽  
Creatinine Ratio ◽  
Urinary Hydroxyproline ◽  
Nigeria Teaching Hospital ◽  
Study Population ◽  
The Mean ◽  
The University

Background: Hydroxyproline is one of the biochemical markers that can be measured objectively as an indicator of normal biological processes or pathological processes. It is usually raised in disease conditions that are associated with bone resorption. Aim: To determine the urinary hydroxyproline/creatinine levels in a study population of healthy Nigerians. Methods: This study recruited 22 consenting participants who served as control for another study at the University of Nigeria Teaching Hospital (UNTH) Ituku-Ozalla, Enugu. All participants were required to fast for at least 12 hours overnight and their early morning second void urine collected between 7am and 8am. The collected urine samples were stored frozen at -20oC until analysis. Colorimetric method of analysis of urinary hydroxyproline and creatinine were done using Biovisionhydroxyproline kit and Randoxcreatinine kit respectively. Bivariate analysis was conducted on the collated data using statistical package of social science (SPSS) version 19. The results were recorded as urinary hydroxyproline alone (μg/μL) and as urinary hydroxyproline/creatinine ratio. Results: The mean urinary hydroxyproline level of 0.020±0.013μg/μL and urinary hydroxyproline/ creatinine ratio of 0.016±0.006 were noted for healthy Nigerians. Conclusions: The urinary hydroxyproline levels in the study population of healthy Nigerians are within normal values reported in other healthy populations.

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