Cytotoxicity and Antimicrobial Activity of the Essential Oil from Satureja montana subsp. pisidica (Lamiceae)

The antimicrobial and cytotoxic activities of the essential oil of Satureja montana ssp. pisidica from two localities (mountains Korab and Galičica) were studied. Forty-nine components were identified in the each sample. Oxygenated monoterpene hydrocarbons were the major compounds: carvacrol, thymol, carvacrol methyl ether and β-linalool. Both tested essential oils showed very high and similar antimicrobial activity. Minimal inhibitory concentrations ranged from 12.5 μg/mL against S. epidermidis to 50 μg/mL against P. aeruginosa and C. albicans. The cytotoxic effect of the essential oils was tested against MDA-MB-361, MDA-MB-453, HeLa, LS174 and MRC5 cells. The essential oil from Korab demonstrated significantly better results than the oil from Galičica, particularly against HeLa and MDA-MB-453 cell lines, with IC50 values of 63.5 and 72.3 μg/mL, while the oil from Galičica was the most active on the human epithelial cervical cancer HeLa cells (IC50 99.7 μg/mL).

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Chemical and Biological Studies of the Essential Oils of Micromelum hirsutum

Natural Product Communications ◽

10.1177/1934578x0700200614 ◽

2007 ◽

Vol 2 (6) ◽

pp. 1934578X0700200

Author(s):

Pham Thi Minh Diep ◽

Agata Maria Pawlowska ◽

Pier Luigi Cioni ◽

Do Huu Nghi ◽

Le Mai Huong ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Cytotoxic Effect ◽

In Vitro Assay ◽

Vitro Assay ◽

Biological Studies ◽

Bacteria And Fungi ◽

Positive Results

Essential oils of the branches and leaves, flowers, and fruits of Micromelum hirsutum (Rutaceae) collected in Vietnam were investigated. Essential oils were obtained by hydrodistillation and analyzed by GC and GC/MS techniques. Essential oils yields were 0.07%, 0.125%, and 0.22% (w/w), respectively, and in each oil 43, 49, and 37 components were identified, comprising 86.0, 86.3, and 96.4% of the total amount, respectively. All the essential oils were screened for antimicrobial activity, showing positive results against various strains of bacteria and fungi. The essential oil of the flowers was also found to have cytotoxic effect against three cancer lines by in vitro assay.

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Effect of matrix pretreatment on the supercritical CO2 extraction of Satureja montana essential oil

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq150504034d ◽

2016 ◽

Vol 22 (2) ◽

pp. 201-209 ◽

Cited By ~ 4

Author(s):

Biljana Damjanovic-Vratnica ◽

Svetlana Perovic ◽

Tiejun Lu ◽

Regina Santos

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Supercritical Co2 ◽

Extraction Yield ◽

Mechanical Compression ◽

Supercritical Co2 Extraction ◽

Mechanical Grinding ◽

Essential Oil Yield ◽

Satureja Montana

The effect of different matrix pretreatment of winter savory(Satureja montana L.) on the supercritical CO2(SC-CO2) extraction - yield, composition and antimicrobial activity of extracts and essential oil (EO) was investigated. Herb matrix was submitted to conventional mechanical grinding, physical disruption by fast decompression of supercritical and subcritical CO2 and physical disruption by mechanical compression. The analyses of the essential oil obtained by SC-CO2 extraction and hydrodistillation were done by GC/FID method. Major compounds in winter savory EO obtained by SC-CO2 extraction and hydrodistillation were: thymol (30.4-35.4% and 35.3%), carvacrol (11.5-14.1% and 14.1%), ?-terpinene (10.2-11.4% and 9.1%) and p-cymene (8.3-10.1% and 8.6%), respectively. The gained results revealed that physical disruption of essential oils glands by fast CO2 decompression in supercritical region (FDS) achieved the highest essential oil yield as well as highest content of thymol, carvacrol and thymoquinone. Antimicrobial activity of obtained winter savory SC-CO2 extracts was the same (FDS) or weaker compared to essential oil obtained by hydrodistillation.

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Evaluation of antioxidant and antimicrobial activity of Ziziphora ‎clinopodiodes essential oil eluted at different times

Current Bioactive Compounds ◽

10.2174/1573407216999200507122039 ◽

2020 ◽

Vol 16 ◽

Author(s):

Mojgan Alizadeh ◽

Akram Arianfar ◽

Ameneh Mohammadi

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Positive Control ◽

Essential Oil Composition ◽

Oil Composition ◽

Citronellic Acid ◽

Antimicrobial And Antioxidant Activity

Objective: Ziziphora clinopodioides is an edible medicinal plant belongs to the Labiatae family that widespread all over Iran. It used as culinary and also in cold and cough treatments in Iran. The aim of present work was to evaluate the effect of different timeframes during the hydrodistillation on essential oil composition, antimicrobial and antioxidant activity. Materials and Methods: The essential oil of Z. clinopodiodes was extracted via hydrodistillation with Clevenger apparatus. The fractions of essential oil were captured at 6 times from the beginning of the distillation: (10, 20, 60, 120, 180 and 240 min). The fractions of essential oil were analyzed by GC/MS and their antibacterial, antifungal and antioxidant activities were studied by Disk - well diffusion and DPPH methods respectively. Results: Six distillation times and whole essential oil were captured during the hydrodistillation. Essential oil yield dropped off significantly during distillation progressed (1.0% for 10 min and 0.025 for 240 min). 1,8 Cineol, Isomenthone, Pulegone, Piperitenone and Citronellic acid were major compounds in fractions and they were affected by distillation times. Pulegone was major compound in all of essential oils. In antioxidant activity assay, whole essential oil was stronger than was stronger than positive control and fractions of essential oil, because of higher levels of Isomenthone, Piperitenone and Citronellic acid. Strongest antimicrobial activity against S. aureus, E. coli and C. albicans was observed from 10 min fraction. Conclusion: Our results indicated that distillation time can create essential oils with specific properties and we can achieve to more efficient essential oil in short times.

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Variability in chemical composition and antimicrobial activity of essential oil of Rosa × damascena Herrm. from mountainous regions of Iran

Chemical and Biological Technologies in Agriculture ◽

10.1186/s40538-021-00219-6 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Mansureh Ghavam ◽

Afsaneh Afzali ◽

Maria Manconi ◽

Gianluigi Bacchetta ◽

Maria Letizia Manca

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Environmental Conditions ◽

Raw Materials ◽

Rosa Damascena ◽

Mountainous Regions ◽

Aspergillus Brasiliensis ◽

Suitable Area

Abstract Background Essential oil of Rosa × damascena Herrm. is one of the most valuable and important raw materials for the flavor and fragrance industry. The cultivation of this plant has ancient origins, and Kashan was one of the first mountainous regions of Iran dealing with the cultivation of R. × damascena. In this study, both chemical composition and antimicrobial activity of different rose essential oils obtained from five mountainous areas of Kashan region (Maragh, Qamsar, Sadeh, Javinan, and Kamoo) has been investigated along with the influence of the environmental conditions on these properties. Results Results showed that yield and chemical composition of essential oils obtained from Rosa × damascena were significantly affected by the collection area. In particular, the yield of oils varied from ~0.08 to ~0.132% and citronellol (36.70-9.18%), geraniol (12.82-0.47%), nonadecane (22.73-10.36%), heneicosane (31.7-11.43%), and 1-nonadecene (6.03-3.93%) have been detected as main compounds in all the plants collected, but at different concentrations depending on the collection area. The best fragrance and the highest yield were found in the oil from Kamoo area. Similarly to the chemical composition, the antimicrobial activity of the essential oils was affected by their origin, and essential oil obtained from plants collected from Kamoo area disclosed the highest antibacterial and antifungal efficacy. Its inhibition halos were 17.33±0.58 mm against Aspergillus brasiliensis, 15.67±0.58 mm against Staphylococcus aureus, and 12.33±0. 58 mm against Streptococcus pyogenes. Essential oils of R. damascena were also effective against Gram-negative Pseudomonas aeruginosa and they had a MIC value of 62.50 μg/mL irrespective of the collection area (except the oil from Javinan area). On the contrary, the highest antifungal power against Candida albicans yeast was reached using the essential oil obtained from plants collected in Javinan region (MIC and MBC ~62.50 μg/mL). Conclusions Overall results underline the influence of environmental conditions of the different areas of Kashan region, on the chemical composition of and antimicrobial activity of the essential oils of Rosa × damascena. In addition, results disclosed that Kamoo seemed to be the most suitable area for the competitive cultivation of R. × damascena to the intensive production of aromatic flower oil and natural antimicrobial essential oils.

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Essential Oils of Four Virginia Mountain Mint (Pycnanthemum virginianum) Varieties Grown in North Alabama

Plants ◽

10.3390/plants10071397 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1397

Author(s):

William N. Setzer ◽

Lam Duong ◽

Trang Pham ◽

Ambika Poudel ◽

Cuong Nguyen ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

University Research ◽

Oil Yield ◽

Research Station ◽

Continuous Extraction ◽

Chromatographic Techniques ◽

Relative Differences ◽

Essential Oil Yield ◽

Very High

Virginia mountain mint (Pycnanthemum virginianum) is a peppermint-flavored aromatic herb of the Lamiaceae and is mainly used for culinary, medicinal, aromatic, and ornamental purposes. North Alabama’s climate is conducive to growing mint for essential oils used in culinary, confectionery, and medicinal purposes. There is, however, a need for varieties of P. virginianum that can be adapted and easily grown for production in North Alabama. Towards this end, four field-grown varieties with three harvesting times (M1H1, M1H2, M1H3; M2H1, M2H2, M2H3; M3H1, M3H2, M3H3, M4H1, M4H2, M4H3) were evaluated for relative differences in essential oil yield and composition. Thirty-day-old greenhouse-grown plants of the four varieties were transplanted on raised beds in the field at the Alabama A & M University Research Station in North Alabama. The plots were arranged in a randomized complete block with three replications. The study’s objective was to compare the four varieties for essential oil yield and their composition at three harvest times, 135, 155, and 170 days after planting (DAP). Essential oils were obtained by hydrodistillation with continuous extraction with dichloromethane using a Likens–Nickerson apparatus and analyzed by gas chromatographic techniques. At the first harvest, the essential oil yield of the four varieties showed that M1H1 had a yield of 1.15%, higher than M2H1, M3H1, and M4H1 with 0.91, 0.76, and 1.03%, respectively. The isomenthone concentrations increased dramatically through the season in M1 (M1H1, M1H2, M1H3) by 19.93, 54.7, and 69.31%, and M3 (M3H1, M3H2, M3H3) by 1.81, 48.02, and 65.83%, respectively. However, it increased only slightly in M2 and M4. The thymol concentration decreased slightly but not significantly in all four varieties; the thymol in M2 and M4 was very high compared with M1 and M3. The study showed that mountain mint offers potential for production in North Alabama. Two varieties, M1 and M3, merit further studies to determine yield stability, essential oil yield, composition, and cultivation development practices.

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Chemical Compositions, Mosquito Larvicidal and Antimicrobial Activities of Essential Oils from Five Species of Cinnamomum Growing Wild in North Central Vietnam

Molecules ◽

10.3390/molecules25061303 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1303 ◽

Cited By ~ 7

Author(s):

Do N. Dai ◽

Nguyen T. Chung ◽

Le T. Huong ◽

Nguyen H. Hung ◽

Dao T.M. Chau ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Larvicidal Activity ◽

Antimicrobial Agents ◽

Chemical Compositions ◽

North Central ◽

Mosquito Larvicidal Activity ◽

Central Vietnam ◽

Leaf Essential Oil

Members of the genus Cinnamomum (Lauraceae) have aromatic volatiles in their leaves and bark and some species are commercially important herbs and spices. In this work, the essential oils from five species of Cinnamomum (C. damhaensis, C. longipetiolatum, C. ovatum, C. polyadelphum and C. tonkinense) growing wild in north central Vietnam were obtained by hydrodistillation, analyzed by gas chromatography and screened for antimicrobial and mosquito larvicidal activity. The leaf essential oil of C. tonkinense, rich in β-phellandrene (23.1%) and linalool (32.2%), showed excellent antimicrobial activity (MIC of 32 μg/mL against Enterococcus faecalis and Candida albicans) and larvicidal activity (24 h LC50 of 17.4 μg/mL on Aedes aegypti and 14.1 μg/mL against Culex quinquefasciatus). Cinnamomum polyadelphum leaf essential oil also showed notable antimicrobial activity against Gram-positive bacteria and mosquito larvicidal activity, attributable to relatively high concentrations of neral (11.7%) and geranial (16.6%). Thus, members of the genus Cinnamomum from Vietnam have shown promise as antimicrobial agents and as potential vector control agents for mosquitoes.

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Antimicrobial Essential Oil Combinations to Combat Foot Odour

Planta Medica ◽

10.1055/a-0592-8022 ◽

2018 ◽

Vol 84 (09/10) ◽

pp. 662-673 ◽

Cited By ~ 4

Author(s):

Ané Orchard ◽

Alvaro Viljoen ◽

Sandy van Vuuren

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Efficacy ◽

Juniperus Virginiana ◽

Broth Microdilution ◽

Synergistic Interactions ◽

Microdilution Method ◽

Broth Microdilution Method

AbstractFoot odour (bromodosis) is an embarrassing and perplexing condition mostly caused by bacteria of the Brevibacterium species. Essential oils are a credible option as an affordable treatment of odour and contribute towards antimicrobial efficacy. Therefore, this study sets out to investigate the antimicrobial activity of essential oil combinations against odour-causing bacteria. The broth microdilution method was used to investigate the antimicrobial activity of 119 essential oil combinations, and the fractional inhibitory index was calculated to determine the interactive profile. Combinations that resulted in synergy in 1 : 1 ratios were further evaluated in different concentrations, and isobolograms were plotted to determine the influence of the ratio on overall activity. Numerous combinations could be identified as having synergistic interactions against the Brevibacterium spp. and no antagonism was observed. The combination of Juniperus virginiana (juniper) and Styrax benzoin (benzoin) demonstrated synergy against all three Brevibacterium spp. tested and J. virginiana was the essential oil responsible for the majority of the synergistic interactions. The results reported here confirm the promising potential of the majority of these oils and selected combinations in treating and controlling bromodosis.

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Chemical composition, antimicrobial, antioxidative and anticholinesterase activity of Satureja Montana L. ssp montana essential oil

Open Life Sciences ◽

10.2478/s11535-014-0298-x ◽

2014 ◽

Vol 9 (7) ◽

pp. 668-677 ◽

Cited By ~ 5

Author(s):

Tatjana Mihajilov-Krstev ◽

Dragan Radnović ◽

Dušanka Kitić ◽

Vesna Jovanović ◽

Violeta Mitić ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Human Serum ◽

Biological Activities ◽

Serum Cholinesterase ◽

Health Improvement ◽

Chemical Compositions ◽

Bacterial Strains ◽

Antimicrobial Potential ◽

Satureja Montana

AbstractThe present study investigates the chemical compositions of three Satureja montana L. ssp montana essential oils and correlates chemical variability with biological activities. GC/MS analysis showed that with an increase in altitude (100–500–800 m), a higher content of linalool, terpinen-4-ol and cis-sabinene hydrate was found, while the percentage of phenolic compounds, thymol and carvacrol decreased. Antimicrobial activity of the essential oils was tested against 7 fungal and 23 bacterial strains. The essential oil characterized by the highest content of phenols and alcohols exhibited the highest antimicrobial potential. The correlation analysis showed that the major carriers of the obtained antioxidant activity are oxygenated monoterpenes. All essential oils inhibited human serum cholinesterase activity. High antimicrobial potential, together with moderate antioxidant capacity and strong inhibition of human serum cholinesterase, classifies S. montana essential oil as a natural source of compounds that can be used in the treatment of foodborne and neurological diseases, wound and other infections, as well as for general health improvement.

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Antimicrobial activities of laboratory produced essential oil solutions against five selected fungal strains

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1324171i ◽

2013 ◽

pp. 171-183 ◽

Cited By ~ 2

Author(s):

Emilija Ivanova ◽

Natalija Atanasova-Pancevska ◽

Dzoko Kungulovski

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Antimicrobial Properties ◽

Microtiter Plate ◽

Antimicrobial Activities ◽

Food Preservation ◽

Minimal Inhibitory Concentrations ◽

Fungal Strains ◽

Treat Ment ◽

Penicillium Spp

It is well known that essential oils possess significant antimicrobial activity. This study was conducted to estimate the antimicrobial activity of various types of Biokill, a laboratory produced solution composed of several essential oils (Biokill dissolved in 96% ethanol; Biokill 96% further dissolved in DMSO; Biokill dissolved in 70% ethanol and Biokill 70% further dissolved in DMSO). The antimicrobial activity was evaluated against five selected fungal strains, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763, Aspergillus niger I.N. 1110, Aspergillus sojae CCF and Penicillium spp. FNS FCC 266. A variation of the microtiter plate-based antimicrobial assay was used in order to assess the antimicrobial activity of the solutions. By applying this assay minimal inhibitory concentrations (MIC) of the Biokill solutions were determined for each strain of the selected test microorganisms. The results demonstrated that all variations of Biokill showed antimicrobial activity at concentrations lower than 2.5?g/mL. Biokill 70% further dissolved in DMSO showed the best antimicrobial properties against all the selected strains with MICs less than 1.25?g/mL. These results indicated that Biokill could find application in the pharmaceutical industry, in food preservation and conservation, in the prevention and treat?ment of plants infected by certain phytopathogens, etc.

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Komposisi Minyak Atsiri dan Aktivitas Antimikroba Rimpang Temu Putih dan Jahe Gajah sebagai Fitobiotik Pakan Unggas

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i2.5560 ◽

2019 ◽

Vol 6 (2) ◽

pp. 181

Author(s):

Laila Nur Rohma ◽

Osfar Sjofjan ◽

M. Halim Natsir

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Feed Additives ◽

Diffusion Method ◽

Disc Diffusion ◽

Activity Test ◽

Main Component

ABSTRAK Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric

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