scholarly journals A Randomized Prospective Study to Assess the Effect of Vitamin E on Peroxiredoxin- 2 As a Novel Antioxidant Marker in Young Beta-Thalassemia Major Patients on Different Chelation Therapy

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 1058-1058
Author(s):  
Mohamed Abd el mohsen ◽  
Maha Saber ◽  
Amira Adly ◽  
Eman Ismail ◽  
Omar Elalfy ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Adjuvant Therapy ◽  
Iron Overload ◽  
Thalassemia Major ◽  
Inclusion Criteria ◽  
Group A ◽  
Baseline Levels ◽  
Group B ◽  
Vitamin E Supplementation

Abstract Introduction: Oxidative stress plays a central role in the pathogenesis of morbidities in β-thalassemia. Peroxiredoxin-2 (PRDX2) is the third most abundant cytoplasmic protein in red cells and is able to reduce and detoxify a vast range of organic peroxides, H2O2, and peroxynitrite. PRDX2 has been demonstrated to be induced by oxidative stress and that cells overexpressing PRDX2 are more resistant to the oxidative stress. Vitamin E is a fat-soluble vitamin and an anti-oxidant which is often depleted in thalassemia patients as a result of iron overload. Aim: This randomized prospective trial aimed to investigate the efficacy and safety of vitamin E as an adjuvant therapy to the three used iron chelators in moderately iron overloaded young vitamin E-deficient β-thalassemia major (β-TM) patients in relation to tissue iron overload and examine its potential corrective value to markers of oxidative stress and to the level of PRDX2 as a novel protective marker of oxidative stress. Methods: Inclusion criteria were β-TM patients aged 6-18 years, vitamin E deficient, serum ferritin (SF) >1000-2500 μg/L and cardiac T2* > 10 ms and ejection fraction > 56 %. A total of 245 β-TM patients were screened for eligibility, 180 were enrolled while 35 did not meet inclusion criteria and 30 were excluded. The 180 β-TM vitamin E-deficient patients were equally enrolled into 3 groups (each; n=60) and received desferrioxamine (DFO), deferiprone (DFP) and deferasirox (DFX). Patients in each group were further randomized either to receive vitamin E supplementation (400 IU daily) or not (n=30). All patients received vitamin E (group A) or no vitamin E (group B) were followed-up for one year with assessment of transfusion index, hemoglobin, serum ferritin (SF), liver iron content (LIC) and cardiac magnetic resonance imaging (MRI) T2*. Malondialdehyde (MDA), as an index for lipid peroxidation, and antioxidants including reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase and PRXD2 were assessed. Results: Baseline vitamin E was negatively correlated to SF, LIC and MDA while it was positively correlated to GSH, GPX, catalase and PRXD2. No significant difference was found as regards the studied variables at baseline and study end in patients who did not receive vitamin E (group B). After vitamin E therapy, transfusion index, SF, and LIC were significantly decreased in group A patients while hemoglobin and cardiac MRI T2* were elevated compared with baseline levels or those in group B without vitamin E. MDA levels were decreased while all the studied antioxidants were increased post-vitamin E supplementation compared with baseline levels or those in group B without vitamin E. The same improvement was found among DFP-treated patients post-vitamin E compared with baseline data. DFP-treated patients had the highest hemoglobin and antioxidant levels with the lowest SF and LIC compared with DFO or DFX subgroups. Conclusions: Vitamin E as an adjuvant therapy possibly potentiates the efficacy of DFP more than DFO and DFX in reducing iron burden and reduces oxidative stress in the moderately iron overloaded vitamin E-deficient β-TM patients, with no adverse events. Disclosures No relevant conflicts of interest to declare.

Anti-oxidative systems in rat skeletal muscle after acute physical exercise

2007 ◽  
Vol 32 (2) ◽  
pp. 190-196 ◽  
Author(s):  
José Alexandre Bachur ◽  
Sérgio Britto Garcia ◽  
Hélio Vannucchi ◽  
Alceu Afonso Jordao ◽  
Paula Garcia Chiarello ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Vitamin E ◽  
Weight Bearing ◽  
Antioxidant Defense System ◽  
Exercise Duration ◽  
The Body ◽  
Group A ◽  
Group B ◽  
Acute Physical Exercise

The objective of this study was to analyze the oxidative stress of skeletal muscle of sedentary rats at the morphological and biochemical level, due to acute physical effort performed at different intensities and during different periods of time. Forty-two male sedentary Wistar rats were divided into two groups, group A (swimming for 50 min) and group B (swimming for 100 min), which were further subdivided into 3 different exercise intensities, non-weight bearing (subgroup I), 3% weight load (subgroup II), and 5% weighted load (subgroup III), as well as a control-rested group (C). The animals were killed by ether inhalation and fragments of the gastrocnemius muscle were removed for the determination of malondialdehyde (MDA), reduced glutathione (GSH), and vitamin E concentrations. When all groups were compared with the control-rested group (C), gastrocnemius MDA levels at 50 and 100 min were higher at all swimming intensities. GSH consumption was greater at all intensities in group A, and only at 100 min in group BI; vitamin E consumption was significantly higher only in groups BII and BIII. When groups were compared by intensity at each swimming time there were no differences between I, II, and III at 50 min for any of the substances analyzed (MDA, GSH, and vitamin E), but at 100 min opposite effects were observed for GSH and vitamin E, with vitamin E consumption and GSH recovery occurring with increasing weight load. Higher concentrations of skeletal muscle MDA could indicate elevated lipid peroxidation at each time and exercise intensity, with highest MDA levels observed after the longest exercise duration and at the highest intensity. The layer-line mode of utilization of body antioxidants could explain the concentrations of GSH and vitamin E: first the use of GSH, even with minor oxidative stress, and then vitamin E consumption only in the presence of greater and more prolonged oxidative stress, indicating the necessity of adequate body levels of both antioxidants for proper utilization by the body antioxidant defense system.

scholarly journals Effects of Dicalcium Phosphate (DCP) and Vitamin E on Growth Performance and Hemato-Biochemical Parameters in Broilers

2020 ◽  
Vol 2 (2) ◽  
pp. p104
Author(s):  
Tairan Ikbal ◽  
Sumitra Saha
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Vitamin E ◽  
Detrimental Effect ◽  
Body Weight Gain ◽  
Experimental Period ◽  
Group A ◽  
Group B ◽  
Vitamin E Supplementation ◽  
Blood Profiles

This study was conducted to assess the effect of DCP and vitamin E supplementation on body weight, hematological- (TEC, Hb content, PCV and ESR) and biochemical (AST and ALT) parameters of “Cobb 500” broiler. A total of 20 chicks (14 days old) were reared and randomly divided into four equal groups (n=5). Body weight was measured at each 7 days interval up to the end of the 35 days of experimental period.  Group A was considered as control, fed with commercial ration. Group B, C and D were treated with 1 gm DCP per kg feed, 1 ml vitamin E per litre of drinking water, and 1 gm DCP per kg feed plus 1 ml vitamin E per litre of drinking water with commercial ration, respectively from day 14 to day 35. It was observed that DCP and vitamin E supplementation significantly (p<0.01) increased body weight. Moreover, TEC and Hb content increased significantly (p<0.01) in the treated groups as compared to control. Besides, ESR, AST and ALT values decreased significantly (p<0.01) in the treated groups. Therefore, it may be concluded that DCP and vitamin E could be used to improve body weight gain and blood profiles without any detrimental effect to liver and muscle on broilers.

scholarly journals Variables associated with malondialdehyde level in thalassemia major patients

2012 ◽  
Vol 52 (3) ◽  
pp. 125
Author(s):  
Arum Gunarsih ◽  
Pustika Amalia ◽  
Imam Boediman
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Iron Overload ◽  
Serum Ferritin ◽  
Medical Records ◽  
Serum Ferritin Level ◽  
Ferritin Level ◽  
Iron Chelation ◽  
Thalassemia Major ◽  
Indirect Measurement

BackgmundThalassemia is the most cormnon hereditary haemolyticanaemia in the world, including in Indonesia. The main treatmentfor thalassemia is regular transfusions, but these are knO\vn to causeiron overload. Moreover, iron overload in jJ􀁮thalassemia patientsgenerates oxygen free radicals and peroxidative lipid injury. Ferritinserum concentration is used as indirect measurement of iron overload.Malondialdehyde (MDA), a terminal compound oflipid peroxidation,is used as an index of oxidative stress status.Objective To assess the correlation between iron overload (serumferritin level) and MDA as a marker of oxidative stress in thalassemiamajor patients.Methods This c ross􀁮sectional study was conducted at CiptoMangunkusumo Hospital, Jakarta, from May􀁮June 2009. Subjectswere thalassemia major patients (homozygous jJ􀁮thalassemia orjJ􀁮thalassemia;HbE) who received regular blood transfusions, iron􀁮chelation, and vitamin E as an antioxidant. Data was collected by his􀁮tory􀁮taking, physical examination, medical records, and questionnaires.Blocd specimens were dra\Vll from the thalassemia major subjects beforetransfusion and examined for serum ferritin and MDA levels.Results Fifty􀁮five subjects Mth thalassemia major (34 homozygousjJ􀁮thalassemia and 21 jJ􀁮thalassemia;HbE) were included in ourstudy. Mean serum ferritin level was 3693.2 (SD 21423),ug/L andme811 MDA level was 0.641 (SD 0.283) nmolimL. No cor relationwas found between serum ferritin and MDA levels in thalassemiamajor subjects (r=0.147, P=0.285). As additional results, this studyalso showed no correlation between MDA to reguler vitamin Econsumption (r=0.277, P=0.028) as well as MDA and nutritionalstatus (F0371, P􀁯0.()J4).Conclusion There was no cor relation between serum ferritin leveland plasma MDA level in thalassemia major subjects, no cor relationsbetween MDA and regular vitamin E consumption, as well as MDAand nutritional status. [paediatr Indones. 2012;52:125,31].

scholarly journals Impact of Oxidative Stress in Premature Aging and Iron Overload in Hemodialysis Patients

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Blanca Murillo-Ortiz ◽  
Joel Ramírez Emiliano ◽  
Wendy Ivett Hernández Vázquez ◽  
Sandra Martínez-Garza ◽  
Sergio Solorio-Meza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Iron Overload ◽  
Telomere Length ◽  
Venous Blood ◽  
Accelerated Aging ◽  
Premature Aging ◽  
Relative Telomere Length ◽  
Group A ◽  
Multiple Blood ◽  
Group B

Background.Increased oxidative stress is a well described feature of patients in hemodialysis. Their need for multiple blood transfusions and supplemental iron causes a significant iron overload that has recently been associated with increased oxidation of polyunsaturated lipids and accelerated aging due to DNA damage caused by telomere shortening.Methods.A total of 70 patients were evaluated concomitantly, 35 volunteers with ferritin levels below 500 ng/mL (Group A) and 35 volunteers with ferritin levels higher than 500 ng/mL (Group B). A sample of venous blood was taken to extract DNA from leukocytes and to measure relative telomere length by real-time PCR.Results.Patients in Group B had significantly higher plasma TBARS (p=0.008), carbonyls (p=0.0004), and urea (p=0.02) compared with those in Group A. Telomeres were significantly shorter in Group B, 0.66 (SD, 0.051), compared with 0.75 (SD, 0.155) in Group A (p=0.0017). We observed a statistically significant association between relative telomere length and ferritin levels (r=-0.37,p=0.001). Relative telomere length was inversely related to time on hemodialysis (r=-0.27,p=0.02).Conclusions.Our findings demonstrate that iron overload was associated with increased levels of oxidative stress and shorter relative telomere length.

Comparative Study Between Clean Intermittent Self-Catheterization (Cisc) and Continuous Indwelling Catheterization (Cidc) in Relieving Acute Refractory Retention of Urine Due to Benign Enlargement of Prostate (Bep)

2020 ◽  
Vol 21 (2) ◽  
pp. 105-110
Author(s):  
Md Shawkat Alam ◽  
Sudip Das Gupta ◽  
Hadi Zia Uddin Ahmed ◽  
Md Saruar Alam ◽  
Sharif Muhammod Wasimuddin
Keyword(s):  
Comparative Study ◽  
Acute Urinary Retention ◽  
Bladder Capacity ◽  
Inclusion Criteria ◽  
College Hospital ◽  
Medical College ◽  
High Incidence ◽  
Group A ◽  
Group B ◽  
Better Than

Objective: To compare the clean intermittent self-catheterization (CISC) with continuous indwelling catheterization (CIDC) in relieving acute urinary retention (AUR) due to benign enlargement of prostate (BEP). Materials and Methods :A total 60 patients attending in urology department of Dhaka Medical college hospital were included according to inclusion criteria ,Patients were randomized by lottery into two groups namely group –A and group –B for CISC and IDC drainage respectively . Thus total 60 patients 30 in each group completed study. Results : Most men can safely be managed as out-patients after AUR due to BPH. The degree of mucosal congestion and inflammation within the bladder was found to be lower in those using CISC and the bladder capacity in these patients was also found higher.Patients with an IDC had a high incidence of UTIs then that of patients with CISC. During the period of catheterization the incidence of UTI was 43.3% in group B in comparison to 40% in group A; before TURP 36% in group B in comparison to 10% incidence in group A.According to patient’s opinion CISC is better than IDC in the management of AUR. Experiencing bladder spasm, reporting blood in urine, management difficulties, incidence and severity of pain were less in CISC group, and the method of CISC was well accepted by patients as well as their family members. Conclusion: From the current study it may be suggested that CISC is better technique for management of AUR patient due to BPH than IDC. It can also be very helpful when surgery must be delayed or avoided due to any reasons in this group of patients. Bangladesh Journal of Urology, Vol. 21, No. 2, July 2018 p.105-110

Gamma Knife surgery as sole treatment for multiple brain metastases: 2-center retrospective review of 1508 cases meeting the inclusion criteria of the JLGK0901 multi-institutional prospective study

2010 ◽  
Vol 113 (Special_Supplement) ◽  
pp. 48-52 ◽  
Author(s):  
Toru Serizawa ◽  
Masaaki Yamamoto ◽  
Yasunori Sato ◽  
Yoshinori Higuchi ◽  
Osamu Nagano ◽  
...  
Keyword(s):  
Brain Metastases ◽  
Gamma Knife ◽  
Class Ii ◽  
Hazard Ratio ◽  
Gamma Knife Surgery ◽  
Inclusion Criteria ◽  
Primary Tumors ◽  
Poor Prognostic Factor ◽  
Group A ◽  
Group B

Object The authors retrospectively reviewed the results of Gamma Knife surgery (GKS) used as the sole treatment for brain metastases in patients who met the eligibility criteria for the ongoing JLGK0901 multi-institutional prospective trial. They also discuss the anticipated results of the JLGK0901 study. Methods Data from 1508 consecutive cases were analyzed. All of the patients were treated at the Gamma Knife House of Chiba Cardiovascular Center or the Mito Gamma House of Katsuta Hospital between 1998 and 2007 and met the following JLGK0901 inclusion criteria: 1) newly diagnosed brain metastases, 2) 1–10 brain lesions, 3) less than 10 cm3 volume of the largest tumor, 4) no more than 15 cm3 total tumor volume, 5) no findings of CSF dissemination, and 6) no impairment of activities of daily living (Karnofsky Performance Scale score < 70) due to extracranial disease. At the initial treatment, all visible lesions were irradiated with GKS without upfront whole-brain radiation therapy. Thereafter, gadolinium-enhanced MR imaging was performed every 2–3 months, and new distant lesions were appropriately retreated with GKS. Patients were divided into groups according to numbers of tumors: Group A, single lesions (565 cases); Group B, 2–4 tumors (577 cases); and Group C, 5–10 tumors (366 cases). The differences in overall survival (OS) were compared between groups. Results The median age of the patients was 66 years (range 19–96 years). There were 963 men and 545 women. The primary tumors were in the lung in 1114 patients, gastrointestinal tract in 179, breast in 105, urinary tract in 66, and other sites in 44. The overall mean survival time was 0.78 years (0.99 years for Group A, 0.68 years for Group B, and 0.62 years for Group C). The differences between Groups A and B (p < 0.0001) and between Groups B and C (p = 0.0312) were statistically significant. Multivariate analysis revealed significant prognostic factors for OS to be sex (poor prognostic factor: male, p < 0.0001), recursive partitioning analysis class (Class I vs Class II and Class II vs III, both p < 0.0001), primary site (lung vs breast, p = 0.0047), and number of tumors (Group A vs Group B, p < 0.0001). However, no statistically difference was detected between Groups B and C (p = 0.1027, hazard ratio 1.124, 95% CI 0.999–1.265). Conclusions The results of this retrospective analysis revealed an upper CI of 1.265 for the hazard ratio, which was lower than the 1.3 initially set by the JLGK0901 study. The JLGK0901 study is anticipated to show noninferiority of GKS as sole treatment for patients with 5–10 brain metastases compared with those with 2–4 in terms of OS.

Glycine and L-Arginine supplementation ameliorates gastro-duodenal toxicity in a rat model of NSAID (Diclofenac)-gastroenteropathy via inhibition of oxidative stress

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Akinleye Stephen Akinrinde ◽  
Halimot Olawalarami Hameed
Keyword(s):  
Oxidative Stress ◽  
Total Protein ◽  
Therapeutic Index ◽  
Control Treatment ◽  
Control Group ◽  
Protective Effects ◽  
Serum Total Protein ◽  
Significant Amelioration ◽  
Group A ◽  
Group B

Abstract Objectives This study examined the possible protective roles of exogenous glycine (Gly) and L-Arginine (l-Arg) against Diclofenac (DIC)-induced gastro-duodenal damage in rats. Methods Rats were divided into Group A (control), Group B (DIC group) and Groups C–F which were pre-treated for five days with Gly1 (250 mg/kg), Gly2 (500 mg/kg), l-Arg1 (200 mg/kg) and l-Arg2 (400 mg/kg), respectively, before co-treatment with DIC for another three days. Hematological, biochemical and histopathological analyses were then carried out. Results DIC produced significant (p<0.05) reduction in PCV (13.82%), Hb (46.58%), RBC (30.53%), serum total protein (32.72%), albumin (28.44%) and globulin (38.01%) along with significant (p<0.05) elevation of serum MPO activity (83.30%), when compared with control. In addition, DIC increased gastric H2O2 and MDA levels by 33.93 and 48.59%, respectively, while the duodenal levels of the same parameters increased by 19.43 and 85.56%, respectively. Moreover, SOD, GPx and GST activities in the DIC group were significantly (p<0.05) reduced in the stomach (21.12, 24.35 and 51.28%, respectively) and duodenum (30.59, 16.35 and 37.90%, respectively), compared to control. Treatment with Gly and l-Arg resulted in significant amelioration of the DIC-induced alterations although l-Arg produced better amelioration of RBC (29.78%), total protein (10.12%), albumin (9.93%) and MPO (65.01%), compared to the DIC group. The protective effects of both amino acids against oxidative stress parameters and histological lesions were largely similar. Conclusions The data from this study suggest that Gly or l-Arg prevented DIC-induced gastro-duodenal toxicity and might, therefore be useful in improving the therapeutic index of DIC.

scholarly journals A comparative clinical study to access the role of Basti and Virechana Karma followed by Rasayana in post menopausal Asthikshaya (Osteoporosis)

2020 ◽  
Vol 5 (05) ◽  
pp. 96-103
Author(s):  
Deepa S. Patil ◽  
Prashanth A.S
Keyword(s):  
Clinical Study ◽  
Inclusion Criteria ◽  
Comparative Clinical Study ◽  
Group A ◽  
Post Menopausal ◽  
Group B

Rajonivrutti condition manifests in the end phase of Jarawastha; but its Samprapti begins from the Sandhikala of Madhyamawastha and Jarawastha due to Vatavruddi in womens reaching Rajonivrutti. Postmenopausal Asthikshaya is a disabling disease, which renders women a bedridden life. Here 40 subjects diagnosed with postmenopausal Asthikshaya fulfilling the inclusion criteria were selected for the study and randomly categorized intp two groups as group A and group B each consisting of 20 subjects. Group A received Amapachana with Hinguvachadivati, Yastimadhu Siddha Ksheerabasti administered in Yoga Basti schedule followed by Tritiyatriphala Rasayana. Group B received Amapachana with Hinguvachadivati, Sadhyosnehapana with Amrita Ghrita, Sarvanga Abyanga with Murchita Tilataila followed by Sarvanga Swedana and Sneha Virechana was admistered with Eranda Taila followed by Tritiyatriphala Rasayana was given. Tritiya Triphala Rasayana selected for the present study by adapting all the general principles of prevention and management of Asthikshaya and as it is a well known Rasayana.

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