Building and Clinical Use of Activated Plasma Clotting Time Test and the Value of APCT on Predicting the Bleeding Due to Leukemia Chemotherapy-Induced Thrombocytopenia.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3915-3915
Author(s):  
Bao-An Chen ◽  
Jin-Jin Li ◽  
Cheng-Yin Huang ◽  
Feng Gao ◽  
Jian Chen ◽  
...  
Keyword(s):  
Sodium Citrate ◽  
Platelet Rich Plasma ◽  
Venous Blood ◽  
Clinical Use ◽  
Clotting Time ◽  
Platelet Suspension ◽  
Platelet Poor Plasma ◽  
Platelet Counts ◽  
Significant Difference ◽  
Time Test

Abstract Objective This study was aimed to determine the optimal concertration of C-PG in activated plasma clotting time (APCT) test and build the APCT test.To investigate the value of activated plasma clotting time test (APCT) on predicting the bleeding due to Leukemia chemotherapy-induced thrombocytopenia. Method We collected healthy blood donors’venous blood, prepared watched platelet suspension and platelet rich plasma in a normal way to which we add different concertrations of C-PG, then test expression of AnnexinV in the surface of activated platelet by FCM. Prepared platelet rich plasma and platelet poor plasma in a normal way to which we add different concertrations of C-PG, then count the plasma clotting time.Forty-one patients with leukemia chemotherapy, twenty-one patients with petechiae or epistaxis or gum bleeding and twenty patients without bleeding symptoms, were involved in the test. Drawed their venous blood and collected in 3.2% buffered sodium citrate(9:1), the platelet counts and APCT, PT, APTT, TT, Fig were assesed. Result Bleeding group’s APCT was obviously prolonged contrast to the non-bleeding group’s. Platelet counts and APCT of the bleeding group and the non-bleeding group were (7.2±2.9) ×109/L, (105.9±8.1)s and (23.0±12.2) ×109/L,(71.4±9.0)s respectively. There was significant difference between the two groups (P<0.01). Using APCT≥90s as the cut-off point to predict the bleeding caused by chemotherapy-induced thrombocytopenia, the sensibility was 100%, and the specificity was 95.7%. Using platelet counts ≤15*109/L, the sensibility and the specificity was 95.2% and 69.0%. PT, APTT, TT, Fig of the bleeding group and the non-bleeding group were 11.7±1.1)S,(31.5±1.3)S,(11.1±1.2)S,(2.37±0.41)g/L and (12.1±0.8)S,(30.8±2.1)S,(10.7±0.9)S,(2.64±0.27)g/L respectively. The results of the two groups were all not significantly different (P>0.05). Conclusion APCT is a good indication of predicting the bleeding due to leukemia chemotherapy -induced thrombocytopenia.

scholarly journals Perbedaan Jumlah Trombosit Platelet Rich Plasma Yang Menggunakan Tabung Natrium Sitrat Dan Tabung ACD-A

2019 ◽  
Vol 5 (1) ◽  
pp. 24-34
Author(s):  
Sheila Clarissa Clarissa ◽  
Jusak Nugraha Nugraha ◽  
Triagung Ruddy
Keyword(s):  
Standard Deviation ◽  
Sodium Citrate ◽  
Platelet Rich Plasma ◽  
T Test ◽  
Control Group ◽  
Average Increase ◽  
Platelet Counts ◽  
Significant Difference ◽  
The Difference ◽  
Paired T Test

Background: PRP is a relatively new field and there is still minor researches that are usage. However, PRP can be applied to a lot of various fields. This study willmake a comparison between tubes that have sodium citrate anticoagulant and tubes that have ACD-A anticoagulants, to be able to find out which of the two are able to produce a higher number of PRPs. Objective: To obtain the anticoagulants that produce more platelet countsand to know the difference in the platelet counts in PRPs found in tubes that have sodium citrate anticoagulants and tubes that have ACD-A anticoagulants. Method: This study was an experimental laboratorium research with an equivalent control group design. Data is normally distributed, so that the parametic statistical analysis test uses Paired T-Test. Results: From 32 respondents, there was an increase in both anticoagulant tubes, where the average increase in platelet counts in PRP using sodium citrate tubes was 337593.75 / μl and the standard deviation was 158795.437 whereas, the average increase in platelet counts in PRP using ACD-A tubes was 909062.50 / μL and the standard deviation is. 284336.005. The difference in platelet counts in PRP using sodium citrate tubes and ACD-A tubes was stated to be significant because the result of the Paired T-Test analysis showed Sig (2-Tailed) = 0,000 (p <0.05). Conclusion: There was a significant difference platelet counts in PRP using sodium citrate tubes and ACD-A tubes. Tubes that have ACD-A anticoagulants had higher results than the ones that have sodium citrate anticoagulant.

scholarly journals The Effect of Exercise on the Total Leukocyte, Absolute Neutrophil, Lymphocyte and Platelet Counts among Sudanese Football Players

2021 ◽  
Vol 11 (6-S) ◽  
pp. 26-32
Author(s):  
Nihad Elsadig Babiker ◽  
Raheeg Mubark Anwer
Keyword(s):  
Physiological Stress ◽  
Hematological Parameters ◽  
Venous Blood ◽  
Age Groups ◽  
The Body ◽  
Case Group ◽  
Football Players ◽  
Platelet Counts ◽  
Significant Difference ◽  
Age Variation

Background: Aerobic exercise induces physiological stress on the body and brings changes in hematological parameters. This study aimed to determine the effect of playing football as an exercise on the total white blood cell count, absolute Neutrophil, lymphocyte and Platelet counts among Sudanese football players. Material and method:  This was a descriptive case control study, done on a number of football players in Khartoum state, Sudan and conducted within the period from August 2021 to October 2021. Three ml of venous blood samples had been collected from every participant in the study, parameters had been measured and calculated using blood cells counter (Sysmex KX-21N).                                                           Results: When compared the TWBCs, Absolute Neutrophil, Lymphocytes and platelet counts between cases and control there was a significant differences with (P ≤0.05). Also there was a significant difference in the case group before and after one hour of exercise with (P =0.00), and in significant differences between different age groups with (P ≥0.05). Conclusion: Playing football for 1 hour cause a significant increase in the TWBCs, absolute Neutrophils, absolute Lymphocytes and Platelets counts. Age variation has no effect on the measured parameters Keywords: Football, TWBCs, Neutrophils, Lymphocytes and Platelets.

In Vitro Biocompatibility of a New High Nitrogen Nickel Free Austenitic Stainless Steel

2007 ◽  
Vol 342-343 ◽  
pp. 605-608 ◽  
Author(s):  
Yi Bin Ren ◽  
Hua Juan Yang ◽  
Ke Yang ◽  
Bing Chun Zhang
Keyword(s):  
Stainless Steel ◽  
Austenitic Stainless Steel ◽  
Blood Compatibility ◽  
Platelet Rich Plasma ◽  
Clotting Time ◽  
Adhesion Test ◽  
High Nitrogen ◽  
In Vitro Biocompatibility ◽  
Time Test

The in vitro blood compatibility of a new nickel free high nitrogen austenitic stainless steel Fe-Cr-Mn-Mo-N (BIOSSN4) was studied by the kinetic clotting time test and the platelet rich plasma adhesion test in this paper. In comparison with 316L stainless steel, the kinetic clotting time of BIOSSN4 steel are longer, and only causes less activation of platelets in platelet adhesion test, which was indicated by their morphology and low spreading. The experimental results reveals that the BIOSSN4 stainless steel has better blood compatibility, the blood compatibility mechanism of steels was analyzed based on surface tension and interfacial tension between the steels and blood.

Reduced Platelet Aggregation in Hemolytic-Uremic Syndrome

1980 ◽  
Vol 43 (02) ◽  
pp. 154-157 ◽  
Author(s):  
Bernard S Kaplan ◽  
Jack S C Fong
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Hemolytic Uremic Syndrome ◽  
Early Phase ◽  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
Normal Subjects ◽  
Platelet Poor Plasma ◽  
Platelet Counts ◽  
Uremic Syndrome

SummaryPlatelet aggregation was studied in three patients during the course of the hemolytic-uremic syndrome (HUS) when the platelet count was below 100,000/mm3 and after the platelet count had normalized. Platelet aggregation was examined in response to epinephrine, adenosine diphosphate (ADP) and collagen. Aggregation did not occur in response to epinephrine when the patients were thrombocytopenic but normal tracings were obtained when the platelet counts had returned to normal. In contrast, platelet-rich plasma from normal subjects diluted with platelet-poor plasma from patients to comparable platelet counts, showed normal aggregation responses. This study demonstrates that platelet aggregation is reduced in the early phase of the HUS.

Platelet Factor 3 Activity in Washed Platelets

1973 ◽  
Vol 30 (03) ◽  
pp. 557-566 ◽  
Author(s):  
S Renaud ◽  
P Gautheron ◽  
H Rosenstein
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Platelet Rich Plasma ◽  
The Other ◽  
Slow Speed ◽  
Platelet Factor ◽  
Platelet Poor Plasma ◽  
Platelet Counts ◽  
Edta Solution ◽  
Layer Chromatography

SummaryPlatelets collected with an EDTA solution and simply washed in an incomplete Tyrode’s presented clotting times in the recalcification (man and rat) and the Stypven (rat) tests that were practically identical to those of the PRP when slow speed centrifugation was used (800 G in man, 1000 G in rat). This was demonstrated, in 6 pools of 5 rats each and in 6 men, by comparing the clotting activity of the citrated platelet-rich plasma to that of the platelets washed and resuspended in the citrated platelet-poor plasma, for platelet counts ranging from 1 × 105 to 10 × 105/mm3. In contrast, centrifugation of platelets at 3000 G markedly affected these clotting activities, as was shown in an additional study comprising 6 pools of 3 rats.Finally, the clotting activity of platelets totally disrupted by sonication appears to be identical quantitatively in both man and rats to that of the total phospholipids extracted from these platelets and separated from the other lipids by thin-layer chromatography and resuspended in plasma by sonication.

scholarly journals Automated Optical Counting of Blood Platelets

Blood ◽  
1971 ◽  
Vol 38 (4) ◽  
pp. 422-430 ◽  
Author(s):  
GEOFFREY M. BRITTIN ◽  
SHIRLEY A. DEW ◽  
ELVI K. FEWELL
Keyword(s):  
Whole Blood ◽  
Blood Platelets ◽  
Venous Blood ◽  
Human Platelets ◽  
Blood Samples ◽  
Platelet Counts ◽  
Large Numbers ◽  
Significant Difference ◽  
Electronic Method ◽  
Electronic Counting

Abstract We have evaluated the use of an optical particle counter to perform automated platelet counts on whole blood. The erythrocytes were lysed by dilution of whole blood with 2 M urea and the remaining platelets and leukocytes were enumerated by a darkfield microscope optical system that detects light diffracted by them. A suspension of fixed human platelets available commercially was highly satisfactory for standardization. The method gave accurate and reproducible platelet counts, comparable with those of electronic particle counting on venous blood and substantially more reliable platelet counts on thrombocytopenic and finger-puncture blood samples. We believe that errors resulting from the electronic method were caused by technical difficulties of sample handling and not to an intrinsic error in electronic counting. By using the automated optical method we found no significant difference between the platelet counts of capillary and venous blood, although capillary platelet counts had twice the variability of venous counts. The optical technique has important advantages over electronic platelet counting, and its superiority appears to be due to the ability to count platelets in diluted whole blood rather than in plasma. It should prove especially useful in performing the large numbers of platelet counts on thrombocytopenic and finger-puncture blood samples that are increasingly important for management of patients receiving chemotherapy.

A Critical Investigation into the Existence of Circulating Platelet Aggregates

1986 ◽  
Vol 56 (01) ◽  
pp. 045-049 ◽  
Author(s):  
A R Saniabadi ◽  
G D O Lowe ◽  
R Madhok ◽  
K Spowart ◽  
B Shaw ◽  
...  
Keyword(s):  
Whole Blood ◽  
Ex Vivo ◽  
Platelet Rich Plasma ◽  
Venous Blood ◽  
White Blood Cells ◽  
Blood Platelet ◽  
Blood Collection ◽  
Significant Difference ◽  
Platelet Aggregates

SummaryBy a method of counting single platelets in diluted whole blood, platelet aggregates were quantified ex-vivo. Four groups: 20 thrombotic patients, 10 non-thrombotic patients, 10 healthy old controls and 10 healthy young controls were included in the study. Using a 19 gauge needle, with and without tubing, venous blood was taken into buffered EDTA, as a disaggregating agent and buffered EDTA-formalin, as the fixative. The amount of platelet aggregates quantified was affected by the quality of venepuncture or the rate of blood flow through the needle, but was unaffected by the presence of the tubing. There was no statistically significant difference between the four groups, in terms of the platelet aggregates quantified, but scanning electron microscopy revealed the presence of irreversible aggregates, composed of platelet red and white blood cells, in the blood of a greater number of thrombotic patients than non-thrombotic or healthy controls. Platelet aggregates were also quantified in aliquots of platelet rich plasma, and were found to be significantly greater than the corresponding values in whole blood. The difference appeared to be due to increased viscosity of the plasma, induced by the fixative which reduces platelet mobility during centrifugation. It is concluded that the platelet aggregates which disaggregate in bufffered EDTA may represent an artifact of blood collection; the irreversible aggregates are suspected to represent the in vivo circulating aggregates.

scholarly journals Platelet Augmentation Potential of Polyherbal Formulation in Cyclophosphamide-Induced Thrombocytopenia in Wistar Rats

Folia Medica ◽  
2021 ◽  
Vol 63 (1) ◽  
pp. 67-73
Author(s):  
Girish Sailor ◽  
Komal Hirani ◽  
Ghanshyam Parmar ◽  
Rajesh Maheshwari ◽  
Rupa Singh ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Bleeding Time ◽  
Study Data ◽  
Clotting Time ◽  
Platelet Counts ◽  
Polyherbal Formulation ◽  
Significant Difference ◽  
Wbc Count ◽  
Later Phase

Introduction: Thrombocytopenia is a condition characterized by abnormally low levels of thrombocytes, also known as platelets, in the blood. Several medicinal plants possess curative and protective effect against thrombocytopenia associated with diseases or drugs. Aim: In the present study, we have investigated the platelet augmentation activity of polyherbal formulation (VITA PLAT Capsule) in cyclophosphamide-induced thrombocytopenic rat model.&nbsp; Materials and methods: Twenty-four albino Wistar rats were divided into four groups. Thrombocytopenia was induced in the rats by administering cyclophosphamide (25 mg/kg, i.p.) for three days to all the groups except normal controls. The test groups were given orally a polyherbal formulation suspended in normal saline for 14 days. Blood was withdrawn from the retro-orbital plexus of the rats on days 1, 7, and 14 of study to determine platelet counts in all groups. Clotting time and bleeding time were determined on the last day of study. Data were collected and analyzed using GraphPad Prism 8.&nbsp; Results: The results showed that the polyherbal formulation treatment could significantly ameliorate platelet count in thrombocyto-penic rats in the initial as well as in the later phase. The total WBC count was also improved during later phase in test groups. However, there is no significant difference between clotting time and bleeding time in all groups.&nbsp; Conclusions: Our study suggests a potential role of this formulation in the augmentation of platelet counts in various thrombocyto-penic disorders including a role in ameliorating the haemorrhagic complications of dengue fever.

scholarly journals Comparative evaluation of platelet augmentation activity of Carica papaya leaf juice and hydrocortisone in thrombocytopenic rats

2015 ◽  
Vol 30 (2) ◽  
pp. 32-40 ◽  
Author(s):  
Tansena Akhter ◽  
Md Ismail Khan ◽  
Eliza Omar Eva
Keyword(s):  
Platelet Count ◽  
Carica Papaya ◽  
Test Group ◽  
Peak Level ◽  
Potential Candidate ◽  
Clotting Time ◽  
Platelet Counts ◽  
Average Value ◽  
Significant Difference ◽  
Leaf Juice

Aim The study aims at determining the possible effects of Carica papaya leaf juice and hydrocortisone in increasing the platelet count in thrombocytopenia similar to dengue fever. Methodology The study was carried out on 24 Long Evans Norwegian strain healthy rats to investigate the comparative platelet increasing effect of Carica papaya leaf juice and hydrocortisone in experimentally induced thrombocytopenic rats. Thrombocytopenia was induced by 3 doses of cyclophosphamide (100mg/kg body weight) given subcutaneously. The rats were divided into 4 groups comprising of six animals in each group. These groups included a normal control and a cyclophosphamide induced thrombocytopenic control and the remaining two cyclophosphamide induced thrombocytopenic groups were administered Carica papaya leaf juice and hydrocortisone respectively. The duration of study was 15 days. The clotting time was determined on the 15th day although blood samples were obtained from the rat tails at various time intervals to determine the platelet count. Collected data was tabulated and Statistical analysis was performed by using appropriate significant test. A probability value P < 0.05 was considered to be statistically significant. Result Average platelet counts of the test and control groups were (6.89)×105/?L and (6.75)×105/?L respectively before the experiment. In the experiment there was no significant difference in the platelet counts during the first 3 days in either group. However, platelet counts within the test group started to fall after Day 3 and remarkable thrombocytopenia developed after 7days and the average value was (2.68)×105/?L. From day 11 Carica papaya leaf juice and Hydrocortisone was given in two test groups following cyclophosphamide and platelet counts observed to reach a peak level at Day 14 (7.83)×105/?L in case of following Carica papaya leaf juice, and (4.05)×105/?L in case of following Hydrocortisone. The clotting time was determined on day 15 and it was found to be considerably lower (95.5 ± 2.762 sec) in case of Carica papaya leaf juice than (129±3.124 sec) in Hydrocortisone. Conclusion Carica papaya leaf juice has more platelet increasing effect than hydrocortisone and this study also reflects that Carica papaya leaf juice may be a potential candidate for further research leading to the development of a herbal therapeutic agent for thrombocytopenia manifested in diseases such as dengue. DOI: http://dx.doi.org/10.3329/bjpp.v30i2.22681 Bangladesh J Physiol Pharmacol 2014; 30(2): 32-40

Interaction Of Plasma Proteins With Artificial Surfaces With Reference To Platelet Adhesion

1981 ◽  
Author(s):  
O P Malhotra ◽  
M N Helmus ◽  
D F Gibbons
Keyword(s):  
Plasma Proteins ◽  
Platelet Adhesion ◽  
Platelet Rich Plasma ◽  
Gel Filtration ◽  
Platelet Suspension ◽  
Platelet Poor Plasma ◽  
Artificial Surfaces ◽  
Glass Surfaces

On occasion, fewer platelets from platelet rich plasma (PRP), adhered to hydrophilic (glass) surfaces exposed to platelet poor plasma (PPP) for 3 min than areas exposed for 3 s. The decrease was dramatic and consistent when platelet suspension (gel-PLS, obtained from PRP by 2B Sepharose gel filtration) was used instead of PRP. To further explore the factors which influence platelet adhesion, we used the following: for surfaces, a) sparkleen-cleaned glass (hydrophilic), b) acid-washed (somewhat hydrophobic), and c) siliconized (hydrophobic); for proteins, a) PPP, b) fibrinogen (96% clottable), c) defi-brinogenated (defib.) plasma, and d) defib. plasma plus fibrinogen; for platelet suspension, a) PRP, b) gel-PLS, and c) platelets in defib. plasma (defib. PLS).From gel-PLS, non-siliconized surfaces exposed to fibrinogen for 3 s attached more platelets (F<0.05) than those exposed to PPP or defib. plasma plus fibrinogen. The latter two attracted more platelets (P<0.01) than defib. plasma. Hydrophilic sparkleen-cleaned glass previously exposed to PPP (or defib. plasma plus fibrinogen) attached a minimum of 10-fold as many platelets from gel-PLS than from PRP. Under similar conditions acid-cleaned surfaces attached 2-fold from gel-PLS, while hydrophobic glass did not show any change. By exposing the surfaces to PPP followed by gel-PLS, the sparkleen-cleaned glass showed the greatest decrease (P<0.001) in the number of platelets attached to areas exposed to PPP for 3 min as compared to 3 s, while siliconized showed no such decrease. If, however, the surfaces were exposed to defib. plasma, they all showed decreases in platelet attraction at 3 min.

Sign in / Sign up

Export Citation Format

Share Document