Supplemental magnolol or honokiol attenuates adverse effects in broilers infected with Salmonella pullorum by modulating mucosal gene expression and the gut microbiota

Abstract Background Salmonella pullorum is one of the most harmful pathogens to avian species. Magnolol and honokiol, natural compounds extracted from Magnolia officinalis, exerts anti-inflammatory, anti-oxidant and antibacterial activities. This study was conducted to evaluate the effects of dietary supplemental magnolol and honokiol in broilers infected with S. pullorum. A total of 360 one-day-old broilers were selected and randomly divided into four groups with six replicates: the negative control group (CTL), S. pullorum-infected group (SP), and the S. pullorum-infected group supplemented with 300 mg/kg honokiol (SPH) or magnolol (SPM). Results The results showed that challenging with S. pullorum impaired growth performance in broilers, as indicated by the observed decreases in body weight (P < 0.05) and average daily gains (P < 0.05), along with increased spleen (P < 0.01) and bursa of Fabricus weights (P < 0.05), serum globulin contents, and the decreased intestine villus height and villus/crypt ratios (P < 0.05). Notably, supplemental magnolol and honokiol attenuated these adverse changes, and the effects of magnolol were better than those of honokiol. Therefore, we performed RNA-Seq in ileum tissues and 16S rRNA gene sequencing of ileum bacteria. Our analysis revealed that magnolol increased the α-diversity (observed species, Chao1, ACE, and PD whole tree) and β-diversity of the ileum bacteria (P < 0.05). In addition, magnolol supplementation increased the abundance of Lactobacillus (P < 0.01) and decreased unidentified Cyanobacteria (P < 0.05) both at d 14 and d 21. Further study confirmed that differentially expressed genes induced by magnolol and honokiol supplementation enriched in cytokine-cytokine receptor interactions, in the intestinal immune network for IgA production, and in the cell adhesion molecule pathways. Conclusions Supplemental magnolol and honokiol alleviated S. pullorum-induced impairments in growth performance, and the effect of magnolol was better than that of honokiol, which could be partially due to magnolol’s ability to improve the intestinal microbial and mucosal barrier.

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Effect of chewing betel nut on the gut microbiota of Hainanese

PLoS ONE ◽

10.1371/journal.pone.0258489 ◽

2021 ◽

Vol 16 (10) ◽

pp. e0258489

Author(s):

Li Ying ◽

Yunjia Yang ◽

Jun Zhou ◽

Hairong Huang ◽

Guankui Du

Keyword(s):

Gut Microbiota ◽

16S Rrna Gene Sequencing ◽

Control Group ◽

Rrna Gene ◽

Betel Nut ◽

Positive Effects ◽

Α Diversity ◽

Host Health ◽

Betel Nut Chewing ◽

Rrna Gene Sequencing

Betel nut chewing (BNC) is prevalent in South Asia and Southeast Asia. BNC can affect host health by modulating the gut microbiota. The aim of this study is to evaluate the effect of BNC on the gut microbiota of the host. Feces samples were obtained from 34 BNC individuals from Ledong and Lingshui, Hainan, China. The microbiota was analyzed by 16S rRNA gene sequencing. BNC decreased the microbial α-diversity. Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria were the predominant phyla, accounting for 99.35% of the BNC group. The Firmicutes-to-Bacteroidetes ratio was significantly increased in the BNC group compared to a control group. The abundances of the families Aerococcaceae, Neisseriaceae, Moraxellaceae, Porphyromonadaceae, and Planococcaceae were decreased in the BNC/BNC_Male/BNC_Female groups compared to the control group, whereas the abundances of Coriobacteriaceae, Streptococcaceae, Micrococcaceae, Xanthomonadaceae, Coxiellaceae, Nocardioidaceae, Rhodobacteraceae, and Succinivibrionaceae were increased. In general, the gut microbiome profiles suggest that BNC may have positive effects, such as an increase in the abundance of beneficial microbes and a reduction in the abundance of disease-related microbes. However, BNC may also produce an increase in the abundance of disease-related microbes. Therefore, extraction of prebiotic components could increase the beneficial value of betel nut.

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Garlic skin induced shifts in the rumen microbiome and metabolome of fattening sheep

10.21203/rs.3.rs-60203/v1 ◽

2020 ◽

Author(s):

Wen Zhu ◽

Zhen Su ◽

Wei Xu ◽

Hanxue Sun ◽

Jinfeng Gao ◽

...

Keyword(s):

Growth Performance ◽

Average Daily Gain ◽

Basal Diet ◽

Diversity Indices ◽

Rumen Fermentation ◽

Control Group ◽

Rumen Microbiome ◽

Volatile Fatty Acid Concentration ◽

Α Diversity ◽

Daily Gain

Abstract Background Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This study was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, ruminal microbes and metabolites in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. In addition, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

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Gut microbiota composition does not associate with Toxoplasma infection in rats

10.22541/au.163898245.55631982/v1 ◽

2021 ◽

Author(s):

Patrick Taggart ◽

Craig Liddicoat ◽

Wen Han Tong ◽

Martin Breed ◽

Philip Weinstein ◽

...

Keyword(s):

Bacterial Community ◽

Community Composition ◽

Bacterial Community Composition ◽

Chronic Phase ◽

Negative Control ◽

Control Group ◽

Rrna Gene ◽

Gastrointestinal Microbiota ◽

Toxoplasma Infection ◽

Behavioural Changes

Toxoplasma infection in intermediate host species closely associates with inflammation. This association has led to suggestions that the behavioural changes associated with infection may be indirectly driven by the resulting sustained inflammation rather than a direct behavioural manipulation by the parasite. If this is correct, sustained inflammation in chronically infected rodents should present as widespread changes in the gastrointestinal microbiota due to the dependency between the composition of these microbiota and sustained inflammation. We conducted a randomized controlled experiment in rats that were assigned to a Toxoplasma-treatment, placebo-treatment or negative control group. We sacrificed rats during the chronic phase of infection, collected their cecal stool samples and sequenced the V3-V4 region of the 16S rRNA gene to characterise the bacterial community in these samples. Toxoplasma infection did not induce widespread changes in the bacterial community composition of the gastrointestinal tract of rats. Rather, we found sex differences in the bacterial community composition and only minor changes in Toxoplasma infected rats. We conclude that it is unlikely that sustained inflammation is the mechanism driving the highly specific behavioural changes observed in Toxoplasma-positive rats.

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Performance and nutrient digestibility of broiler chickens fed graded levels of fermented and toasted seed meal

Nigerian Journal of Animal Production ◽

10.51791/njap.v45i2.458 ◽

2020 ◽

Vol 45 (2) ◽

pp. 150-156

Author(s):

D. N. Tsado ◽

A. H. Dikko ◽

O. J. Alabi ◽

T. N. Kperun ◽

B. K. Jiya ◽

...

Keyword(s):

Growth Performance ◽

Feed Intake ◽

Broiler Chickens ◽

Nutrient Digestibility ◽

Seed Meal ◽

Control Group ◽

Albizia Lebbeck ◽

Final Body Weight ◽

Randomized Design ◽

Better Than

This study was conducted to investigate the effects of fermented and toasted Albizia lebbeck seed meal (FTALSM), on the growth performance and nutrient digestibility of broiler chickens at the starter and finisher phases. One hundred and fifty (150) day old chicks (Abo acres) were used for this study and were divided into five treatments (with three replicate each containing 10 birds) using a completely randomized design (CRD) for eight weeks. The treatments were five diets containing 0% (control), 5%, 10%, 15% and 20% of fermented and toasted Albizia lebbeck seed meal (FTALSM), respectively and were tagged Diet 1, 2, 3, 4 and 5. The diets were formulated to be isocaloric and isonitrogenous. The feed intake at the starter phase was significantly higher (p<0.05) in Diets 3 and 4 which had 10 and 15% inclusion of FTALSM, respectively. Diets 2, 3 and 4 also differ significantly (p<0.05) in final body weight and total weight gained from the control diets. The results at the finisher phase indicated that, there were significant differences (P<0.05) in all the parameters measured except for mortality. Birds fed Diet 2 performed significantly better in weight gain (1453g) and feed intake (3087.25g) compared to the control group. Birds on the control and Diet 2 had similar feed efficiency, the values were however, better than those on the other treatments. The results of nutrient digestibility showed birds fed Diet 5 (20% FTALSM) recorded significantly (P<0.05) low digestibility values in all parameters measured. The results showed that fermentation and toasting reduced phytase, oxalate and cyanide by 41.87, 26.67 and 99.94 % respectively. It can be concluded therefore, that FTALSM can be included up to 15 % in broiler chickens starter diets and 5 % in the finisher diet for optimum growth performance.

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Mucosa-Associated Microbiota in Patients with Irritable Bowel Syndrome: A Comparison of Subtypes

Digestion ◽

10.1159/000512167 ◽

2020 ◽

pp. 1-8

Author(s):

Hiroshi Matsumoto ◽

Akiko Shiotani ◽

Ryo Katsumata ◽

Shinya Fukushima ◽

Yukiko Handa ◽

...

Keyword(s):

Irritable Bowel Syndrome ◽

Sigmoid Colon ◽

Terminal Ileum ◽

Lysine Biosynthesis ◽

Control Group ◽

Rrna Gene ◽

Unifrac Distance ◽

Linear Discriminant ◽

Α Diversity ◽

Irritable Bowel

Background: Most studies on gut microbiome of irritable bowel syndrome (IBS) have focused on fecal microbiota, instead of mucosa-associated microbiota (MAM). Aims: The aim of this study wasto investigate the MAM in IBS patients including the difference in subtypes of IBS, namely, diarrhea-predominant IBS (IBS-D) and constipation-predominant IBS (IBS-C). Methods: Endoscopic brush samples were taken from terminal ileum and sigmoid colon of patients with IBS (17 IBS-D patients and 7 IBS-C patients) and 10 healthy controls. The MAM of samples was profiled by 16S rRNA gene amplicon sequencing. Potential changes in the MAM at the functional level were evaluated using PICRUSt software and the KEGG database. Results: There were no differences in MAM composition between terminal ileum and sigmoid colon according to β-diversity based on the UniFrac distance. In view of α-diversity, Shannon (evenness) but not Chao1 (richness) or observed operational taxonomic units tended to be lower in sigmoid colon MAM of IBS-C and IBS-D than the control group. The abundance of 4 genera in the sigmoid colon and 7 genera in the terminal ileum was significantly different among the 3 groups. Linear discriminant analysis effect size (LEfSe) showed that the genera of Ruminococcus, Akkermansia, Butyrivibrio, Methylobacterium, and Microbacterium and the family Erysipelotrichaceae were significantly higher in the IBS-C group, and the abundance of the genera Streptococcus, Acidaminococcus, Butyricicoccus, and Parvimonas was significantly higher in the IBS-D group. In addition, the proportion of genes responsible for the secretion system and LPS biosynthesis was significantly higher and that for methane metabolism, lysine biosynthesis, and enzyme families was significantly lower in the IBS-D group than in the IBS-C group. Conclusion: Dysbiosis pattern and the function of the microbiome seem to be different among subtypes of IBS, and MAM may play a crucial role in IBS symptom generation.

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Alterations in Vaginal Microbiota and Associated Metabolome in Women with Recurrent Implantation Failure

mBio ◽

10.1128/mbio.03242-19 ◽

2020 ◽

Vol 11 (3) ◽

Author(s):

Min Fu ◽

Xiaowei Zhang ◽

Yiheng Liang ◽

Shouren Lin ◽

Weiping Qian ◽

...

Keyword(s):

Embryo Transfer ◽

Inositol Phosphate ◽

Clinical Pregnancy ◽

Vaginal Microbiota ◽

Control Group ◽

Rrna Gene ◽

Implantation Failure ◽

Recurrent Implantation Failure ◽

Critical Feature ◽

Α Diversity

ABSTRACT Recurrent implantation failure (RIF) refers to repeated failure to become pregnant after transferring embryos with normal morphology. However, the pathogenesis of RIF remains unrevealed, especially for those without any pathological features. In this study, we characterized the vaginal microbiota and metabolomes of patients with unexplained RIF, while patients who achieved clinical pregnancy in the first frozen embryo transfer (FET) cycle were used as controls. Based on 16S rRNA gene sequencing of the vaginal microbiota, the vaginal Lactobacillus showed a significant positive correlation with the pregnancy rate, and the RIF group presented higher microbial α-diversity than the control group (P value = 0.016). The metabolomic profile identified 2,507 metabolites, of which 37 were significantly different between the two groups (P value < 0.05, variable importance for the projection [VIP] > 1). Among them, 2′,3-cyclic UMP and inositol phosphate were the top two metabolites that were higher in the RIF group, while glycerophospholipids and benzopyran were important metabolites that were lower in the RIF group. A lack of lysobisphosphatidic acid and prostaglandin metabolized from glycerophospholipids will lead to deferred implantation and embryo crowding. Benzopyran, as a selective estrogen receptor modulator, may affect the outcome of pregnancy. All of the changes in metabolite profiles may result in or from the differential microbiota compositions in RIF patients. In conclusion, significant differences were presented in the vaginal microbiota and metabolomes between patients with unexplained RIF and women who became pregnant in the first FET cycle. For the first time, this study elaborates the possible pathogenesis of RIF by investigating the vaginal microbiota and metabolites in RIF patients. IMPORTANCE In vitro fertilization-embryo transfer (IVF-ET) is now widely applied for treating infertility, and unexplained recurrent implantation failure (RIF) has become a substantial challenge. We hypothesize that vaginal microbial dysbiosis is associated with RIF, as it is linked to many female reproductive diseases. In this study, we characterized the vaginal microbiota and metabolomes of patients with unexplained RIF, while patients who achieved clinical pregnancy in the first IVF cycle were set as controls. In general, significant differences were discovered in the vaginal microbiota and metabolomes between the two groups. This study is the first detailed elaboration of the vaginal microbiota and metabolites associated with RIF. We believe that our findings will inspire researchers to consider the dynamics of microbiomes related to the microenvironment as a critical feature for future studies of nosogenesis not only for RIF but also for other reproductive diseases.

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A Study of the Effectiveness of Hwanggeum-tang and Gamchosasim-tang in the Mice Model of Inflammatory Bowel Disease

The Journal of Internal Korean Medicine ◽

10.22246/jikm.2021.42.3.351 ◽

2021 ◽

Vol 42 (3) ◽

pp. 351-374

Author(s):

Young-kwang Kim ◽

Young-ho Moon

Keyword(s):

Inflammatory Bowel Disease ◽

Bowel Disease ◽

Negative Control ◽

Mucosal Barrier ◽

Raw 264.7 Cells ◽

Control Group ◽

No Production ◽

Protein Activation ◽

Inflammatory Bowel

Objectives: This study investigates the mechanism of Hwanggeum-tang (HGT) and Gamchosasim-tang (GST) on inflammatory bowel disease (IBD).Methods: The mice (C57BL/6N) were treated with distilled water and 3% dextran sulfate sodium (DSS) to experimentally induce ulcerative colitis. The mice were divided into 7 groups of (6 mice: normal, negative control, positive control (with sulfasalazine), 4 experimental groups (with HGT and GST, respectively). RAW 264.7 cells were used for cell experiments. The experiment was conducted in two ways: in vitro and in vivo.Results: In the experimental group (HGT, GST) of in vitro experiments, NO production decreased, and significant changes in gene expression and protein activation were observed. The length of the colon recovered in the experimental groups (HGT, GST) of the in vivo experiment was longer than that of the negative control group, and the mucosal barrier was recovered. Sone significant changes in the amount of mRNA expression were partially observed, and significant changes in protein activation also were confirmed.Conclusions: HGT and GST are effective in treating IBD caused by DSS. In the same herbal preparation group, the higher the concentration, the better the experimental effect, and when the same concentration was tested, HGT was more effective than GST. Herbal medicine has a higher antioxidant effect than sulfasalazine, so it is also excellent for cell protection.

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Selection, characterisation and evaluation of potential probiotic Lactobacillus spp. isolated from poultry droppings

Beneficial Microbes ◽

10.3920/bm2015.0020 ◽

2016 ◽

Vol 7 (1) ◽

pp. 35-44 ◽

Cited By ~ 9

Author(s):

S. Asghar ◽

M. Arif ◽

M. Nawaz ◽

K. Muhammad ◽

M.A. Ali ◽

...

Keyword(s):

Immune Response ◽

Body Weight ◽

Weight Gain ◽

Body Weight Gain ◽

Negative Control ◽

The Body ◽

Control Group ◽

Rrna Gene ◽

Poultry Droppings

Aim of the present study was to characterise and evaluate probiotic potential of lactobacilli isolated from indigenous poultry. Lactobacilli were isolated from poultry droppings and identified by genus specific polymerase chain reaction and 16S rRNA gene sequencing. Isolates were characterised in vitro by their ability to tolerate low pH and bile salts, phytase activity, antimicrobial activity, antibiotic susceptibility profile, and autoaggregation and coaggregation with poultry gut pathogens. In vivo evaluation of selected isolates was done by their effect on the body weight gain and immune response of broiler chicks. Total of 90, one-day old chicks, were randomly divided in 9 groups and given selected lactobacilli alone and in combinations (108 cfu/bird, daily) from day 7 to day 35. Body weight gain and humoral immune response to New Castle Disease Virus (NDV) vaccine were determined weekly. Three lactobacilli isolates (SMP52, SMP64 and SMP70) were selected as potentially probiotic bacteria on the basis of in vitro characterisation and identified as Lactobacillus crispatus, Lactobacillus casei and L. crispatus, respectively. Chicks supplemented with ‘SMP52’, ‘SMP64’, ‘SMP70’ and ‘SMP64+SMP70’ and a commercial probiotic product (Protexin) showed significantly higher mean weight gain per bird (1,584±35.2, 1,629±30.6, 1,668±34.7, 1,619±29.5 and 1,576±31.7 g/bird, respectively) as compared to negative control group (1,394±26.7 g/bird), on day 35. SMP 70 also showed significantly higher geometric mean titre against NDV vaccine at day 21 as compared to negative control. It is concluded that L. crispatus SMP52, L. casei SMP64 and L. crispatus SMP70 are potential probiotic candidates which alone or in different combinations may increase body weight of broilers.

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Potential role of intestinal microflora in disease progression among patients with different stages of Hepatitis B

Gut Pathogens ◽

10.1186/s13099-020-00391-4 ◽

2020 ◽

Vol 12 (1) ◽

Author(s):

Xiu-An Yang ◽

Fengchun Lv ◽

Ran Wang ◽

Yange Chang ◽

Yiming Zhao ◽

...

Keyword(s):

Hepatitis B ◽

Intestinal Microflora ◽

Potential Role ◽

Control Group ◽

Rrna Gene ◽

Faecal Microbiota ◽

Faecal Microbiota Transplantation ◽

Potential Biomarker ◽

Α Diversity

Abstract Background Increasing evidence demonstrate that the gut microbiota is involved in the pathogenesis of liver diseases, and faecal microbiota transplantation is considered to be a promising new treatment option. However, there are no reports on the intestinal flora of asymptomatic HBV carriers using next-generation sequencing. This study intends to investigate the potential role of the intestinal microflora in predicting the progression of Hepatitis B patients in different non-cancerous stages. Results A total of 266 patients with different stages of Hepatitis B and 31 healthy controls were included in this study. Some of the subjects (217 cases) underwent 16S rRNA gene sequencing. Compared with the control group (CK), the α diversity of patients in Group A (HBV carrier) slightly increased, while that of patients in the other three groups decreased. Each group of patients, especially those in Group C (cirrhosis) and Group D (acute-on-chronic liver failure), could be separated from the CK using weighted UniFrac PCoA and ANOSIM. LEfSe revealed that 40 taxa belonging to three phyla had an LDA larger than 4. In addition to the comparison between Group B (chronic Hepatitis B) and Group C, the specific flora and potential taxonomic function were also identified. Different microbial communities were found to be highly correlated with clinical indicators and the Child-Pugh scores. Changes in the microbial community were highly related to the alternations of host metabolism, which in turn, was related to the development of Hepatitis B. Our analysis identified a total of 47 strains with potential biomarker functions at all levels except for the phylum level. Conclusions Faecal microbiota transplantation of some potential beneficial bacteria can change with the occurrence of disease, and HBV carriers might be the most suitable donors.

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The High Level of Xylooligosaccharides Improves Growth Performance in Weaned Piglets by Increasing Antioxidant Activity, Enhancing Immune Function, and Modulating Gut Microbiota

Frontiers in Nutrition ◽

10.3389/fnut.2021.764556 ◽

2021 ◽

Vol 8 ◽

Author(s):

Jiaman Pang ◽

Xingjian Zhou ◽

Hao Ye ◽

Yujun Wu ◽

Zhenyu Wang ◽

...

Keyword(s):

Immune Function ◽

Growth Performance ◽

Average Daily Gain ◽

Dietary Supplementation ◽

Control Group ◽

Feed Conversion ◽

Beneficial Bacteria ◽

Α Diversity ◽

High Level ◽

Weaning Piglets

The aim of this study was to investigate the effects of the high level of xylooligosaccharides (XOS) on growth performance, antioxidant capability, immune function, and fecal microbiota in weaning piglets. The results showed that 28 d body weight exhibited linear and quadratic increases (P < 0.05) with increasing dietary XOS level, as well as average daily feed intake (ADFI) on d 15–28, average daily gain (ADG) on d 15–28 and 1–28. There was a linear decrease (P < 0.05) between XOS levels and feed conversion rate (FCR) on d 1–14 and 1–28. Additionally, glutathione peroxidase (GSH-Px) showed a linear increase (P < 0.05), while the malondialdehyde (MDA) level decreased linearly and quadratically (P < 0.05) with the increasing dietary level of XOS. Moreover, the XOS treatments markedly increased the levels of immunoglobulin A (Ig A) (linear, P < 0.05; quadratic, P < 0.05), IgM (quadratic, P < 0.05), IgG (linear, P < 0.05), and anti-inflammatory cytokine interleukin-10 (IL-10) (quadratic, P < 0.05) in serum, while the IL-1β (linear, P < 0.05; quadratic, P < 0.05) and IL-6 (linear, P < 0.05) decreased with increasing level of XOS. Microbiota analysis showed that dietary supplementation with 1.5% XOS decreased (P < 0.05) the α-diversity and enriched (P < 0.05) beneficial bacteria including Lactobacillus, Bifidobacterium, and Fusicatenibacter at the genus level, compared with the control group. Importantly, linearly increasing responses (P < 0.05) to fecal acetate, propionate, butyrate, and total short-chain fatty acids (SCFAs) were observed with increasing level of XOS. Spearman correlation analyses found that Lactobacillus abundance was positively correlated with ADG, acetate, propionate, and IgA (P < 0.05), but negatively correlated with IL-1β (P < 0.05). Bifidobacterium abundance was positively related with ADFI, total SCFAs, IgG, and IL-10 (P < 0.05), as well as g_Fusicatenibacter abundance with ADFI, total SCFAs, and IL-10. However, Bifidobacterium and Fusicatenibacter abundances were negatively associated with MDA levels (P < 0.05). In summary, dietary supplementation with XOS can improve the growth performance in weaning piglets by increasing antioxidant capability, enhancing immune function, and promoting beneficial bacteria counts.

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