scholarly journals Structural characterization of polysaccharide from jujube (Ziziphus jujuba Mill.) fruit

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaolong Ji ◽  
Yanqiu Cheng ◽  
Jingyuan Tian ◽  
Siqi Zhang ◽  
Yongshuai Jing ◽  
...  
Keyword(s):  
High Performance ◽  
Gel Permeation Chromatography ◽  
Structural Features ◽  
Resonance Spectroscopy ◽  
Acidic Polysaccharide ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ziziphus Jujuba ◽  
Ft Ir ◽  
Fast Flow

Abstract Background Jujube (Ziziphus jujuba Mill.) fruit is one of the largest productions in China and its increasing production has drawn considerable attention from researchers. Polysaccharide is one of the most abundant components of jujube, and it represents a major group of biolotegically active constituents. This study intended to investigate the special structure of a homogeneous acidic polysaccharide (PZMP4) produced from Ziziphus Jujuba cv. Muzao fruit using novel methods, including DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography. Results The structure of PZMP4 was determined via high-performance gel permeation chromatography (HPGPC), gas chromatography (GC), Fourier transform infrared spectroscopy (FT-IR), methylation analysis, nuclear magnetic resonance spectroscopy (NMR), scanning electron microscopy (SEM), and atomic force microscopy (AFM). The results reveal that PZMP4 with a molecular weight of 27.90 kDa was composed of rhamnose, arabinose, mannose, glucose, galactose, and galacturonic acid at a ratio of 2.32:2.21:0.22:0.88:2.08:8.83. Advanced structural analysis revealed a netted structure with molecular aggregates of PZMP4. Structural features demonstrated that the basic backbone of PZMP4 appeared to mainly consist of (1→4)-linked GalpA with three branches bonded to O-3 of (1→3)-linked Araf, (1→2)-linked Rhap, and terminated with GalpA. Conclusions PZMP4’s unique structure could imply distinct bioactivities and have considerable utilization in functional food. Graphic abstract

scholarly journals Preparation, Thermal, and Thermo-Mechanical Characterization of Polymeric Blends Based on Di(meth)acrylate Monomers

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 878
Author(s):  
Krystyna Wnuczek ◽  
Andrzej Puszka ◽  
Łukasz Klapiszewski ◽  
Beata Podkościelna
Keyword(s):  
Mechanical Analysis ◽  
Attenuated Total Reflection ◽  
Scanning Calorimetry ◽  
Force Microscopy ◽  
Chemical Structures ◽  
Atomic Force ◽  
Polymeric Blends ◽  
Ft Ir ◽  
Acrylate Monomers ◽  
Synthesized Materials

This study presents the preparation and the thermo-mechanical characteristics of polymeric blends based on di(meth)acrylates monomers. Bisphenol A glycerolate diacrylate (BPA.GDA) or ethylene glycol dimethacrylate (EGDMA) were used as crosslinking monomers. Methyl methacrylate (MMA) was used as an active solvent in both copolymerization approaches. Commercial polycarbonate (PC) was used as a modifying soluble additive. The preparation of blends and method of polymerization by using UV initiator (Irqacure® 651) was proposed. Two parallel sets of MMA-based materials were obtained. The first included more harmless linear hydrocarbons (EGDMA + MMA), whereas the second included the usually used aromatic copolymers (BPA.GDA + MMA). The influence of different amounts of PC on the physicochemical properties was discussed in detail. Chemical structures of the copolymers were confirmed by attenuated total reflection–Fourier transform infrared (ATR/FT-IR) spectroscopy. Thermo-mechanical properties of the synthesized materials were investigated by means of differential scanning calorimetry (DSC), thermogravimetric (TG/DTG) analyses, and dynamic mechanical analysis (DMA). The hardness of the obtained materials was also tested. In order to evaluate the surface of the materials, their images were obtained with the use of atomic force microscopy (AFM).

Effect of Catalysts on the Properties of Fluorinated Polyurethanes

2013 ◽  
Vol 464 ◽  
pp. 9-13 ◽  
Author(s):  
Zan Li ◽  
Xia Wang ◽  
Ying Li ◽  
Wei Chain ◽  
Jiao Jiao Hu
Keyword(s):  
Hard Segment ◽  
Soft Segment ◽  
Force Microscopy ◽  
Atomic Force ◽  
Structure Surface ◽  
Improved Stability ◽  
Ft Ir ◽  
Tin Metal ◽  
Polyether Polyol ◽  
Catalyst Efficiency

Fluorinated polyurethanes (FPU) was prepared using fluorinated polyether polyol (FPO) as the soft segment, 4,4`-diphenylmethane diisocyanate (MDI) as the hard segment, 1,4-butanodiol (BDO) as the chain extender and catalysts. Tin metal catalysts were used to catalyze the polyurethane reaction of polyether polyols and isocyanate. The effect of different catalysts including stannous octoate (T-9) and dibutyltindalautrate (DBTDL) on the structure, surface properties and thermal properties of FPU was studied. The structural elucidation of the synthesized FPU was performed by Fourier transform infrared (FT-IR) and discovered that with decreasing catalyst efficiency or without catalyst, the strength of hydrogen bounds were enhanced. The FPU films surface was characterized by contact angle (CA) and atomic force microscopy (AFM) and it was found that the phase separation was increasing with increasing catalyst efficiency. The thermal property was exhibited by Thermo gravimetric (TG) and showed that joining catalyst improved stability significantly.

scholarly journals Synthesis of amphiphilic ABA triblock oligomer via ATRP and its surface properties

2017 ◽  
Vol 95 (5) ◽  
pp. 605-611 ◽  
Author(s):  
Lei Wang ◽  
Shaoqing Wen ◽  
Zhanxiong Li
Keyword(s):  
Photoelectron Spectroscopy ◽  
Microphase Separation ◽  
Gel Permeation Chromatography ◽  
Contact Angles ◽  
Chemical Compositions ◽  
Water Contact ◽  
Force Microscopy ◽  
Atomic Force ◽  
Poly Ethylene ◽  
Magnetic Resonances

A series of novel amphiphilic ABA-type poly(tridecafluorooctylacrylate)-poly(ethylene glycol)-poly(tridecafluorooctylacrylate) (henceforth referred to as p-TDFA-PEG-p-TDFA) triblock oligomers were successfully synthesized via atom transfer radical polymerization (ATRP) using well-defined Br-PEG-Br as macroinitiator and copper as catalyst. The block oligomers were characterized by Fourier transform infrared (FTIR) spectroscopy and 1H and 19F nuclear magnetic resonances (NMR). Gel permeation chromatography (GPC) showed that the block oligomers have been obtained with narrow molecular weight distributions of 1.22–1.33. X-ray photoelectron spectroscopy (XPS) was carried out to confirm the attachment of p-TDFA-PEG-p-TDFA onto the silicon substrate, together with the chemical compositions of p-TDFA-PEG-p-TDFA. The wetabilities of the oligomer films were measured by water contact angles (CAs). Water CAs of p-TDFA-PEG-p-TDFA film were measured and their morphologies were tested by atomic force microscopy (AFM). The result showed that the CAs of the oligomer films, which possess fluoroalkyl groups assembled on the outer surface, increase after heating due to the migration of fluoroalkyl groups and the resulted microphase separation of the p-TDFA-PEG-p-TDFA.

scholarly journals Lectins at Interfaces—An Atomic Force Microscopy and Multi-Parameter-Surface Plasmon Resonance Study

Materials ◽  
2018 ◽  
Vol 11 (12) ◽  
pp. 2348 ◽  
Author(s):  
Katrin Niegelhell ◽  
Thomas Ganner ◽  
Harald Plank ◽  
Evelyn Jantscher-Krenn ◽  
Stefan Spirk
Keyword(s):  
Atomic Force Microscopy ◽  
Surface Plasmon Resonance ◽  
Protein Adsorption ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Resonance Spectroscopy ◽  
Carbohydrate Binding ◽  
Force Microscopy ◽  
Atomic Force ◽  
Slow Kinetics

Lectins are a diverse class of carbohydrate binding proteins with pivotal roles in cell communication and signaling in many (patho)physiologic processes in the human body, making them promising targets in drug development, for instance, in cancer or infectious diseases. Other applications of lectins employ their ability to recognize specific glycan epitopes in biosensors and glycan microarrays. While a lot of research has focused on lectin interaction with specific carbohydrates, the interaction potential of lectins with different types of surfaces has not been addressed extensively. Here, we screen the interaction of two specific plant lectins, Concanavalin A and Ulex Europaeus Agglutinin-I with different nanoscopic thin films. As a control, the same experiments were performed with Bovine Serum Albumin, a widely used marker for non-specific protein adsorption. In order to test the preferred type of interaction during adsorption, hydrophobic, hydrophilic and charged polymer films were explored, such as polystyrene, cellulose, N,-N,-N-trimethylchitosan chloride and gold, and characterized in terms of wettability, surface free energy, zeta potential and morphology. Atomic force microscopy images of surfaces after protein adsorption correlated very well with the observed mass of adsorbed protein. Surface plasmon resonance spectroscopy studies revealed low adsorbed amounts and slow kinetics for all of the investigated proteins for hydrophilic surfaces, making those resistant to non-specific interactions. As a consequence, they may serve as favorable supports for biosensors, since the use of blocking agents is not necessary.

scholarly journals Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1610 ◽  
Author(s):  
Jia Li ◽  
Chao Cai ◽  
Mengmeng Zheng ◽  
Jiejie Hao ◽  
Ya Wang ◽  
...  
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Gel Permeation Chromatography ◽  
Monosaccharide Composition ◽  
Biological Properties ◽  
Orthogonal Experimental Design ◽  
Lentinus Edodes ◽  
Anion Exchange Column ◽  
Strong Anion Exchange ◽  
Fast Flow

The purpose of this study is to develop a robust approach to obtain β glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization. The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study. After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations. LeP-N2 was found to be composed of (1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains. Atomic force microscopy (AFM) and high-performance gel permeation chromatography–refractive index–multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution. This (1→6)-β-d-glucan was tested for antioxidant activities with IC50 at 157 μg/mL. Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL. These findings suggest that the (1→6)-β-d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.

scholarly journals Optical, Structural and Electrical Properties of Electrochemical Synthesis of Thin Film of Polyaniline

2018 ◽  
Vol 15 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
X Ray Diffraction ◽  
X Ray ◽  
Force Microscopy ◽  
Morphological Studies ◽  
Atomic Force ◽  
Vis Spectroscopy ◽  
Structural And Electrical Properties ◽  
A Cell ◽  
Ft Ir ◽  
Ir And Raman

Polyaniline membranes of aniline were produced using an electrochemical method in a cell consisting of two poles. The effect of the vaccination was observed on the color of membranes of polyaniline, where analysis as of blue to olive green paints. The sanction of PANI was done by FT-IR and Raman techniques. The crystallinity of the models was studied by X-ray diffraction technique. The different electronic transitions of the PANI were determined by UV-VIS spectroscopy. The electrical conductivity of the manufactured samples was measured by using the four-probe technique at room temperature. Morphological studies have been determined by Atomic force microscopy (AFM). The structural studies have been measured by (SEM).

Synthesis of Macromolecular Coupling Agent and its Effects on Polystyrene Composites

2014 ◽  
Vol 1053 ◽  
pp. 268-275
Author(s):  
Hong Wen Zhang ◽  
Shi Long Zhou ◽  
Yang Zhang ◽  
Yan Jiang ◽  
Qiang Yu
Keyword(s):  
Composite Materials ◽  
Gel Permeation Chromatography ◽  
Ultraviolet Spectrum ◽  
Butyl Methacrylate ◽  
Coupling Agents ◽  
Resonance Spectroscopy ◽  
H Nmr ◽  
Comprehensive Performance ◽  
Ft Ir ◽  
The Impact

Different molecular weight of block coupling agents with well-defined structures have been synthesized successfully by atom transfer radical polymerization (ATRP) from styrene (St), butyl methacrylate (BMA) and 3-methoxyacryloyl-propyltrimethoxyl silicon (KH-570) are as monomer. The structures and compositions of macromolecular coupling agents have been characterized by means of infrared spectrum (FT-IR), ultraviolet spectrum (UV), nuclear magnetic resonance spectroscopy (1H-NMR) and gel permeation chromatography (GPC). And their effects on the polystyrene/silica (PS/SiO2) composite materials have been studied. The results show that interface compatibility and mechanical properties of composite materials containing macromolecule coupling agents are improved significantly. The composite materials with block macromolecular coupling agents possess more excellent comprehensive performance. Furthermore, the impact strength increased by 110% when comparing with composite materials which are not modified by the coupling agents.

scholarly journals Nanostructured Chitosan/Maghemite Composites Thin Film for Potential Optical Detection of Mercury Ion by Surface Plasmon Resonance Investigation

Polymers ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1497
Author(s):  
Nurul Illya Muhamad Fauzi ◽  
Yap Wing Fen ◽  
Nur Alia Sheh Omar ◽  
Silvan Saleviter ◽  
Wan Mohd Ebtisyam Mustaqim Mohd Daniyal ◽  
...  
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Mercury Ion ◽  
Resonance Spectroscopy ◽  
Force Microscopy ◽  
Sensing Applications ◽  
Atomic Force ◽  
Fe2o3 Thin Film

In this study, synthesis and characterization of chitosan/maghemite (Cs/Fe2O3) composites thin film has been described. Its properties were characterized using Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and ultraviolet-visible spectroscopy (UV-Vis). FTIR confirmed the existence of Fe–O bond, C–N bond, C–C bond, C–O bond, O=C=O bond and O–H bond in Cs/Fe2O3 thin film. The surface morphology of the thin film indicated the relatively smooth and homogenous thin film, and also confirmed the interaction of Fe2O3 with the chitosan. Next, the UV-Vis result showed high absorbance value with an optical band gap of 4.013 eV. The incorporation of this Cs/Fe2O3 thin film with an optical-based method, i.e., surface plasmon resonance spectroscopy showed positive response where mercury ion (Hg2+) can be detected down to 0.01 ppm (49.9 nM). These results validate the potential of Cs/Fe2O3 thin film for optical sensing applications in Hg2+ detection.

scholarly journals Interactions and Dissociation Constants of Galactomannan Rendered Cellulose Films with Concavalin A by SPR Spectroscopy

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3040
Author(s):  
Pilar Vilaró ◽  
Carina Sampl ◽  
Gundula Teichert ◽  
Werner Schlemmer ◽  
Mathias Hobisch ◽  
...  
Keyword(s):  
Active Sites ◽  
Dissociation Constants ◽  
Resonance Spectroscopy ◽  
Irreversible Adsorption ◽  
Force Microscopy ◽  
Cellulose Films ◽  
The Past ◽  
Physiological Processes ◽  
Atomic Force ◽  
Dissociation Constant Kd

Interactions of biomolecules at interfaces are important for a variety of physiological processes. Among these, interactions of lectins with monosaccharides have been investigated extensively in the past, while polysaccharide-lectin interactions have scarcely been investigated. Here, we explore the adsorption of galactomannans (GM) extracted from Prosopis affinis on cellulose thin films determined by a combination of multi-parameter surface plasmon resonance spectroscopy (MP-SPR) and atomic force microscopy (AFM). The galactomannan adsorbs spontaneously on the cellulose surfaces forming monolayer type coverage (0.60 ± 0.20 mg·m−2). The interaction of a lectin, Concavalin A (ConA), with these GM rendered cellulose surfaces using MP-SPR has been investigated and the dissociation constant KD (2.1 ± 0.8 × 10−8 M) was determined in a range from 3.4 to 27.3 nM. The experiments revealed that the galactose side chains as well as the mannose reducing end of the GM are weakly interacting with the active sites of the lectins, whereas these interactions are potentially amplified by hydrophobic effects between the non-ionic GM and the lectins, thereby leading to an irreversible adsorption.

Using Demercurated Lighting Phosphor and MSWI Scrubber Residues to Prepare High Performance Plastic Concrete

2009 ◽  
Vol 610-613 ◽  
pp. 55-60
Author(s):  
Wu Jang Huang ◽  
Wei Chu ◽  
Ling Hui Hsieh ◽  
Jian Guo Chen
Keyword(s):  
Thermal Properties ◽  
Epoxy Resin ◽  
Solid Waste ◽  
High Performance ◽  
Waste Incineration ◽  
Type I ◽  
Phosphor Powder ◽  
Force Microscopy ◽  
Atomic Force ◽  
Plastic Concrete

This study aimed to prepare a high performance plastic concrete made of epoxy resin and Portland type-I cement mixed with at least one inorganic solid waste of demercurated lighting phosphor powder or municipal solid waste incineration scrubber residue. The ratio between liquid epoxy resin and cement was 1:2; the scrubber residue and demercurated phosphor powder were added as modifiers for cement component in order to improve the strength and thermal properties of synthesized plastic concrete. The results indicate that, the addition of scrubber residue causes a decrease in both strength and thermal properties; whereas, the demercurated phosphor powder can replace 100% of the contents of cement without any significantly change in either strength or thermal properties. Atomic force microscopy and Raman spectroscopy were used to characterize the chemical structure of cured concrete and the results indicate that the surface softness increases with an increase in the mixed percentage of epoxy resin.

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