Biomechanical and biochemical evaluation of the effect of systemic application of omeprazole on the osseointegration of titanium implants

Abstract Background This study aimed to investigate the effects of systemic omeprazole treatment on the osseointegration of titanium implants. Material and methods After surgical insertion of titanium implants into the metaphyseal part of rats’ both right and left tibial bones, the animals were randomly divided into three equal groups: control (n = 8), omeprazole dosage-1 (n = 8) (OME-1), and omeprazole dosage-2 (n = 8) (OME-2) and totally 48 implants were surgically integrated. The rats in the control group received no treatment during the four-week postoperative experimental period. In the OME-1 and OME-2 groups, the rats received omeprazole in doses of 5 and 10 mg/kg, respectively, every 3 days for 4 weeks. After the experimental period, the rats were euthanized. One rat died in each group and the study was completed with seven rats in each group. Blood serum was collected for biochemical analysis, and the implants and surrounding bone tissue were used for biomechanical reverse-torque analysis. In the biomechanical analysis, implants that were not properly placed and were not osseointegrated were excluded from the evaluation. Results One-way analysis of variance and Tukey’s honestly significant difference test and Student’s t test were used for statistical analysis. The reverse-torque test (control (n = 9), OME-1 (N = 7), and OME-2 (n = 7)) analysis of biochemical parameters (alkaline phosphatase, calcium, phosphorus, aspartate aminotransferase, alanine amino transferase, urea, and creatinine) revealed no significant differences between the groups (control (n = 7), OME-1 (N = 7), and OME-2 (n = 7)) (P > 0.05). Conclusions Omeprazole had no biomechanical or biochemical effects on the osseointegration process of titanium implants.

Download Full-text

Influence of the Thermal Treatment to Address a Better Osseointegration of Ti6Al4V Dental Implants: Histological and Histomorphometrical Study in a Rabbit Model

BioMed Research International ◽

10.1155/2018/2349698 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 12

Author(s):

Antonio Scarano ◽

Ezio Crocetta ◽

Alessandro Quaranta ◽

Felice Lorusso

Keyword(s):

Thermal Treatment ◽

Dental Implants ◽

Bone Healing ◽

Rabbit Model ◽

Titanium Implants ◽

Control Group ◽

Left Femur ◽

Bone Response ◽

First Choice ◽

Significant Difference

Background. Pure titanium continues to be the first choice for dental implants and represents the gold standard for their biocompatibility and physical and mechanical characteristics, while the titanium alloy (Ti6Al4V) has good mechanical properties. The surface structure of the titanium oxide layer formation on the surface influences and improves the bone response around dental implants. Purpose. The purpose of this study is to evaluate the influence of a thermal treatment of Ti6Al4V implant surfaces and the bone healing response in a rabbit model. Methods. Altogether sixteen implants with same design were inserted into the distal femoral metaphysis. A screw (13 mm long, 4 mm in diameter) was inserted in an implant bed. Each rabbit received two implants, one in the left femur and one in the right femur. The samples were histologically and histomorphometrically evaluated at 8 weeks. Results. A statistically significant difference (p = 0.000034) was present histologically in the percentages of bone-implant contact (BIC) between the test group (BIC = 69.25±4.49%.) and control group (BIC = 56.25 ± 4.8%) by one-way analysis of variance (ANOVA). Significance was set at p ≤ 0.05. Conclusions. The outcome of the present study indicates a novel approach to improving bone healing around titanium implants.

Download Full-text

Effect of Titanium Implants Coated with Radiation-Crosslinked Collagen on Stability and Osseointegration in Rat Tibia

Materials ◽

10.3390/ma11122520 ◽

2018 ◽

Vol 11 (12) ◽

pp. 2520 ◽

Cited By ~ 4

Author(s):

Eun-Bin Bae ◽

Ji-Hyun Yoo ◽

Sung-In Jeong ◽

Min-Su Kim ◽

Youn-Mook Lim ◽

...

Keyword(s):

Gamma Radiation ◽

Human Mesenchymal Stem Cells ◽

Region Of Interest ◽

Bone Volume ◽

Titanium Implants ◽

Control Group ◽

Gene Expressions ◽

Significant Difference

This study aimed to evaluate the titanium (Ti) implants coated with collagen type Ⅰ crosslinked using gamma-irrigation or glutaraldehyde (GA). The in vitro surface observations, quantification assay, and cell studies using human mesenchymal stem cells (hMSCs) were conducted. For in vivo experiments, the implants were divided into three groups and inserted into the rat tibias: control group (non-treated Ti implant), GA group (Ti implants coated with GA-crosslinked collagen) and 25 kGy group (Ti implants coated with gamma-radiation-crosslinked collagen at dose of 25 kGy). The animals were sacrificed at 4 weeks after implantation and the tissue sections were obtained. New bone volume (mm3) and bone-to-implant contact (BIC, %) within the region of interest (ROI) was measured. The in vitro results showed the highest osteogenic differentiation and levels of osteogenesis-related gene expressions in the 25 kGy group without cytotoxicity. The new bone volume of GA group was significantly higher than the control (p < 0.05). In the result of the BIC, the 25 kGy group was significantly higher than the control (p < 0.05). However, there was no significant difference between the experimental groups. Within the limitations of this study, Ti implant coated with gamma-radiation-crosslinked collagen has potential utility without side effects from chemical agents.

Download Full-text

Cardiovascular and hematologic effects produced by chronic treatment with etoricoxib in normotensive rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502009000300008 ◽

2009 ◽

Vol 24 (3) ◽

pp. 206-210 ◽

Cited By ~ 1

Author(s):

Nilo César do Vale Baracho ◽

Guilherme Pedrosa Guizelli ◽

Beatriz Leone Carmello ◽

Danielle de Souza Sanches ◽

Felipe Moraes Costa Silva ◽

...

Keyword(s):

Blood Cells ◽

Chronic Treatment ◽

Experimental Period ◽

Control Group ◽

Histopathological Changes ◽

Study Results ◽

Significant Difference ◽

Hematological Effects ◽

The Mean ◽

Hematological Changes

PURPOSE: Evaluate the cardiovascular and hematological effects produced by chronic treatment with two dosis of etoricoxib in Wistar normotensive rats. METHODS: Thirty rats have been used and divided into one control group and two etoricoxib (10mg/kg and 30mg/kg) treatments groups for 60 days. The mean arterial pressure (MAP) was taken during the whole experimental period and at the end of this period, under anesthesia blood samples were taken, and further the withdrawn of the aorta, heart, brain, liver, and kidneys for the anatomopathologic study. RESULTS: The treatment with etoricoxib (30mg/Kg) produced a significant increase of the MAP from the 28th day of the experiment and from the platelets when compared to the control group and to the group treated with 10mg/Kg, besides producing a highly significant difference in hematocrit and in the red blood cells in relation to the control group. On the other hand the treatment with etoricoxib has not caused histopathological changes when compared to the control. CONCLUSION: These data show that the chronic treatment with etoricoxib leads to increase of the MAP, and to important hematological changes which seem to be associated to the hemoconcentration although not producing anatomopathological significant changes.

Download Full-text

Acute and Subacute Toxicological Evaluation of the Aerial Extract ofMonsonia angustifoliaE. Mey. ex. A. Rich in Wistar Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/4952485 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Anthony Jide Afolayan ◽

Olubunmi Abosede Wintola ◽

Gerda Fouche

Keyword(s):

Wistar Rats ◽

Morphological Changes ◽

Plasma Concentrations ◽

Toxicity Testing ◽

Experimental Period ◽

Control Group ◽

Toxicological Evaluation ◽

Subacute Toxicity ◽

Significant Difference ◽

Dose Dependent

The acute and subacute toxicity profile of the aerial extract ofMonsonia angustifoliain Wistar rats was evaluated. The Organization for Economic Cooperation and Development (OECD) 420 guideline was adopted in the acute toxicity testing with a single oral dose of 5000 mg/kg (b.w.). For the 28-day daily oral dosing, the extract was administered at 75, 150, and 300 mg/kg b.w.; 1% ethanol in sterile distilled water was used as control. Clinical toxicity signs were subsequently evaluated. At a single dose of 5000 mg/kg b.w. the extract elicited no treatment-related signs of toxicity in the animals during the 14 days of experimental period. In the subacute toxicity, there was no significant difference in hematological, renal, and liver function indices. However, dose-dependent significant increases were observed on the plasma concentrations of white blood cell and platelet counts of the treated animals compared to the control group. While cage observations revealed no treatment-facilitated signs of toxicity, histopathological examinations of the kidneys and liver also showed no obvious lesions and morphological changes. These results suggest that the extract may be labelled and classified as safe and practically nontoxic within the doses and period of investigation in this study.

Download Full-text

The Influence of Dietary Salt on the Osseointegration of Implants in Aging Rats

International Archives of Otorhinolaryngology ◽

10.1055/s-0039-1693141 ◽

2019 ◽

Vol 23 (04) ◽

pp. e427-e432

Author(s):

Julio Baldisserotto ◽

Dalva Maria Pereira Padilha ◽

José Miguel Amenábar

Keyword(s):

Salt Intake ◽

Test Group ◽

Experimental Period ◽

Saline Group ◽

Normal Diet ◽

Male Rats ◽

Control Group ◽

Dietary Salt ◽

Aging Rats ◽

Significant Difference

Introduction The bone-implant interface has been studied extensively, but only few papers focused on the nutritional aspects that may affect bone quality, especially salt intake. Objective To study the osseointegration of implants in salt-loaded rats with low mineral bone content. Methods A total of 60 4-month-old male rats were divided in 2 groups (n = 30), being these groups divided in 2 periods, (2 and 4 months). The control group received a normal diet, while the test group received a diet supplemented with 1% sodium chloride (NaCl). Implants were placed in the tibia of both groups. A total of 15 animals of each group were sacrificed at the 2nd month of the experiment, while the remaining animals were sacrificed at the 4th month. Results No statistically significant difference was found in food intake between the groups on any experimental period, but a statistically significant difference was found in the liquid intake in the saline group in both periods. For all groups, osseointegration was observed in both groups. The mean percentage of osseointegration in the cortical bone, in the trabecular bone, and in the total osseointegrated surface between the control (46.38 ± 16.17%) and saline (49.13 ± 11.52%) groups at 2 months was not statistically different (p = 0.61). The total osseointegration areas of the control (53.98 ± 12.06%) and saline (51.40 ± 13.01%) groups at the 4th month of the study were not statistically (p = 0.61). Conclusion Ingestion of salt did not affect directly the osseointegration process during the period of the experiment. The results suggest that mineral losses may not affect the achievement of good osseointegration in aging rats.

Download Full-text

Efficacy of a herbal toothpaste on patients with established gingivitis: a randomized controlled trial

Brazilian Oral Research ◽

10.1590/s1806-83242006000200015 ◽

2006 ◽

Vol 20 (2) ◽

pp. 172-177 ◽

Cited By ~ 26

Author(s):

Fabiana Ozaki ◽

Claudio Mendes Pannuti ◽

Ana Vitória Imbronito ◽

Wellington Pessotti ◽

Luciana Saraiva ◽

...

Keyword(s):

Adverse Reactions ◽

Controlled Trial ◽

Test Group ◽

Experimental Period ◽

Positive Control ◽

Control Group ◽

Control Groups ◽

Double Blind ◽

Significant Difference ◽

And Control

The aim of this randomised, double blind controlled trial was to verify the efficacy of a herbal dentifrice on the reduction of plaque and gingivitis. Forty eight volunteers with established gingivitis were randomly assigned to either a test group (herbal dentifrice) or positive control group (dentifrice with triclosan and fluoride). The dentifrices were distributed in plain white tubes by an independent pharmacy, which revealed the contents of each tube only after the experimental period. Plaque and gingivitis assessments were carried out on baseline and after 28 days of product use. All examinations were conducted by the same calibrated investigator. Subjects were instructed to brush their teeth three times daily using their assigned dentifrice for 28 days. There was a significant reduction in plaque levels in both the test and control groups. However, there was no significant difference between the groups. A significant reduction in gingivitis was observed in both groups, although there was no significant difference between them. No adverse reactions were reported. The authors concluded that both dentifrices were effective in reducing plaque and gingivitis in subjects with established gingivitis.

Download Full-text

Comparison of Antimicrobial Efficacy of Calcium Hydroxide and 2% Chlorhexidine as Medicaments

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v5i2.19156 ◽

2018 ◽

Vol 5 (2) ◽

pp. 8-11

Author(s):

Rupan Tripathi ◽

Vanita Gautam ◽

V. K. Pahwa ◽

Vikas Agrhari ◽

Manu Batra ◽

...

Keyword(s):

Calcium Hydroxide ◽

Normal Saline ◽

Antimicrobial Efficacy ◽

Control Group ◽

Medical Sciences ◽

Human Teeth ◽

Honestly Significant Difference ◽

Significant Difference ◽

Post Hoc ◽

Chlorhexidine Gel

INTRODUCTION: The use of intracanal medicaments help in elimination of bacteria that remain even after cleaning and shaping. This study isdone to compare the antimicrobial efficacy of2% chlorhexidine gel and calcium hydroxide as an intracanal medicament against E.faecalis.MATERIAL & METHODS: Sixty extracted single-rooted human teeth were prepared with standard method. After contaminating the canals with E. Faecalis and incubated for seven days, the samples were divided into four groups (n=15). Normal saline was used as control group. The teeth in each group were treated with normal saline, calcium hydroxide and 2% chlorhexidine gel. Microbial samples were obtained from the dentinal shavings of root and colony forming units (CFU) of E.faecalis were recorded after 24hrs, 72hrs and 7 days.RESULTS: To determine the significance of the differences between the different groups, ANOVA(Analysis of variance) and Tukeys Honestly significant Difference (HSD) Post hoc test were performed. Mean CFU of calcium hydroxide group and 2% chlorhexidine group was statistically significantly different from CFU of all other groups (p<0.01).CONCLUSION: Mean CFU of calcium hydroxide was found to increase at 24 hrs and decreases at 72 hrs and 7 days.The antimicrobial efficacy 2% chlorhexidine gel was better than calcium hydroxide paste.Journal of Universal College of Medical Sciences (2017)Vol.05 No.02 Issue 16, Page: 8-11

Download Full-text

Hamster Cheek Pouch Carcinoma: Effect of Incision and Cortisone on Growth, Invasion, and Metastasis

Journal of Dental Research ◽

10.1177/00220345860650061401 ◽

1986 ◽

Vol 65 (6) ◽

pp. 927-931 ◽

Cited By ~ 7

Author(s):

K. Tsiklakis ◽

N.K. Wood ◽

P.D. Toto ◽

D.B. Doemling

Keyword(s):

Distant Metastases ◽

Experimental Period ◽

Cervical Lymph Node Metastasis ◽

Cheek Pouch ◽

Control Group ◽

Hamster Cheek Pouch ◽

Cervical Lymph Nodes ◽

Significant Difference ◽

Cheek Pouches ◽

The Right

After cheek pouch carcinomas were induced in hamsters by the application of dimethylbenzanthracene (DMBA) to the right pouch for 13 weeks, the animals were divided into four groups and observed for seven more weeks. The control group received no further treatment, two experimental groups had incisional biopsies performed on tumors in their pouches, one of these groups also received injections of cortisone throughout the 20-week experimental period, and a fourth group received cortisone only. The wet weights of the cancerous cheek pouches were determined, and the submandibular and parotid salivary glands with associated cervical lymph nodes, the lungs, and the liver were examined with light microscopy. The cancerous pouches of the animals that received cortisone weighed significantly less than those of animals that received no cortisone but had incisional biopsies of the tumors. There was no significant difference in the degree of histodifferentiation of the tumors among the four groups. The animals in the two groups that received cortisone had significantly more tumors that were invasive than did the animals that did not receive cortisone. Cervical lymph node metastasis occurred in 21% to 38% of the animals but was not significantly different in the four groups. Distant metastases to the lungs or the liver were not found. Incisional biopsy of the tumors stimulated local growth of the cheek pouch tumors, and systemic cortisone administration produced more invasive cheek pouch tumors.

Download Full-text

PARATHYROID HORMONE AND BIOCHEMICAL METABOLIC BONE MARKERS IN PATIENTS WITH β-THALASSEMIA MAJOR IN MISAN PROVINCE / IRAQ

Periódico Tchê Química ◽

10.52571/ptq.v17.n35.2020.06_al-zuhairy_pgs_65_72.pdf ◽

2020 ◽

Vol 17 (35) ◽

pp. 65-72

Author(s):

Noor AL-Huda Salah AL-ZUHAIRY ◽

Zainab Abdul Jabbar Ridha AL-ALI

Keyword(s):

Vitamin D ◽

Alkaline Phosphatase ◽

Thalassemia Major ◽

Control Group ◽

Female Patients ◽

Metabolic Bone ◽

Significant Difference ◽

Calcium Phosphorus ◽

Male Patients ◽

Serum Pth

Beta-thalassemia is a heterogeneous group of hereditary blood disorders characterized by defects in the synthesis of the β- chains of hemoglobin, resulting in variable phenotypes ranging from severe anemia to clinically asymptomatic individuals. This study aims to assess the serum PTH, vitamin D, calcium, phosphorus, alkaline phosphatase, and magnesium levels in β-thalassemia major patients. A total of 50 (30 male and 20 female) patients with β- thalassemia major with ages range 11- 16 years and an equal number of sex-matched healthy adolescents as a control group were included in this study. A total of 52% of patients were lived in an urban area, and there was no significant difference between patients and the control group regarding residency. Male patients showed low statistically significant (P 0.05) mean serum PTH, vitamin D, and calcium levels, but mean serum phosphorus and alkaline phosphatase levels were significantly higher (P 0.05) as compared to the male control group. However, female patients had low, but without statistical significant (P>0.05) mean serum PTH level, whereas vitamin D and calcium levels were highly significant (P 0.05) reduced. The phosphorus and ALP levels were highly significantly (P 0.05) increased as compared to female controls. Regarding β- thalassemia major group, the current study showed male patients had non-significant (P 0.05) higher levels of PTH, calcium, phosphorus, and ALP. In contrast, vitamin D level was non-significantly (P 0.05) low in male patients as compared to female patients. Mean serum level of PTH had a negative correlation with phosphorus, but it had a positive association with vitamin D, calcium, ALP, and magnesium. In conclusion, this study demonstrated that β-thalassemia major patients have a markedly deranged biochemical metabolic bone profile. Regular monitoring of PTH and biochemical mineral profile is also recommended.

Download Full-text

The Effects of Integrating E-Media Piano in Teaching Music

International Journal of Applied Science ◽

10.30560/ijas.v1n2p117 ◽

2018 ◽

Vol 1 (2) ◽

pp. p117

Author(s):

Teejay D. Panganiban ◽

John Gabriel G. Contreras ◽

Kenneth D. Malabanan ◽

Jen Patrice A. Sayas

Keyword(s):

Educational Software ◽

Classroom Instruction ◽

Assessment Tool ◽

Experimental Period ◽

Study Groups ◽

T Test ◽

Intellectual Ability ◽

Control Group ◽

Significant Difference ◽

Teaching Music

Historically, schools, universities and adult education providers have used a “lecture-based” teaching model. This approach to learning was developed during the industrial age, some two centuries ago. The concept is for students to sit passively in rows of chairs or tables all facing the presenter, who usually resides at a lectern. A lecture is a “one-to-many” form of communication, involving little or no audience participation. It is authoritarian, by nature. For an information dump a lecture works fine. The development of engaging lecture activities requires a significant amount of instructor preparation and limits the time available to provide traditional lectures. However, the positive results of this study suggest the need for a restructuring of the plain classroom lecture to incorporate more engaging lectures to improve both the qualitative experiences and performance levels of students. Due to the fast changing society and modernization, there is also change in interest of the students learning styles, so the researchers conducted a study about the effect of educational software in teaching music. This research determined the effects of integrating e-media piano in teaching music at Santiago National High School. Specifically, it dealt with the following: the pretest result of the control and experimental group, the post-test of the two groups; and the significant difference between the pretest and posttest results of the two groups. The input of the study was determined by administering the researcher-made test which is the principal tool for gathering the needed data. The gathered data underwent statistical treatments such as mean, standard deviation, t-test for independent means and t-test for dependent means. A pretest was administered from the two study groups before the conduct of the experiment; this is to determine if the groups were equally comparable in terms of intellectual ability. The validated and reliability teacher-made test is composed of twenty-five (25) items. This covered topics about the different elements of music such as melody, tempo, dynamics, rhythm, pitch, harmony, texture, and timbre. Moreover, both groups were given posttest after the experimental period had been conducted. The same 25 multiple choice item tests was given to the experimental and control group to find out if the use of E- Media piano software had an effect to the students’ performance in music. Nevertheless, the latter was superior than the former. Thus, the effectiveness of E-media is notable, enhancing the learners’ capacity for acquisition and retention of information. The integration of e-media piano software to traditional classroom instruction may be encouraged. Educational software specifically designed to fit the learning objectives can be used as motivation, enrichment activity, and as an assessment tool to classroom instruction. The active participation and interest that educational software may provide to the teaching and learning process should be exploited. Educational software made and developed appropriately to the level of the learners may be adopted not only in music but in the other components of M.A.P.E.H as well. Other strategies which may integrate other trends articulated with the interest of today’s learners may also be adapted as exposing students to modernized school can achieve a quality education and can produce lifelong learning.

Download Full-text