Development of a multispectral microscopy platform using laser diode illumination for effective and automatic label-free Plasmodium falciparum detection

2020 ◽  
Author(s):  
Yao Alvarez Kossonou ◽  
Jérémie Zoueu
Keyword(s):  
Light Emitting Diode ◽  
Malaria Diagnosis ◽  
Cell Detection ◽  
Light Sources ◽  
Label Free ◽  
Fluorescence Excitation ◽  
Light Emitting ◽  
Spectral Bands ◽  
Illumination System ◽  
Set Up

In this paper, we present the progress made in developing multimodal and multispectral light microscopy for label-free malaria diagnosis. Our previously developed light emitting diode (LED) illumination system was replaced by laser diodes as light sources in order to narrow the spectral bands and improve the effectiveness of the contrast function for infected blood cell detection. The acquisition system is now equipped with an algorithm for automatic field scanning and best in-focus determination. We demonstrate the potential of this platform to provide multiple investigation modalities like transmission, reflection, scattering, fluorescence, excitation, emission and polarisation. The application of this platform on malaria-infected samples has shown the effectiveness of such a system in label-free and all-optical malaria detection by allowing the possibility of using a different type of imaging set-up for the samples analysed. Also, fewer illumination sources are used to characterise malaria-infected red blood cells compared to our previous works on malaria detection using LEDs illumination sources.

scholarly journals High-Power, Computer-Controlled, Light-Emitting Diode–Based Light Sources for Fluorescence Imaging and Image-Guided Surgery

2009 ◽  
Vol 8 (3) ◽  
pp. 7290.2009.00009 ◽  
Author(s):  
Sylvain Gioux ◽  
Vida Kianzad ◽  
Razvan Ciocan ◽  
Sunil Gupta ◽  
Rafiou Oketokoun ◽  
...  
Keyword(s):  
Light Emitting Diode ◽  
Computer Control ◽  
Light Sources ◽  
Fluorescence Excitation ◽  
Excitation Light ◽  
Light Emitting ◽  
Guided Surgery ◽  
Image Guided ◽  
Nir Fluorescence ◽  
Fluorescence Guided Surgery

Optical imaging requires appropriate light sources. For image-guided surgery, in particular fluorescence-guided surgery, a high fluence rate, a long working distance, computer control, and precise control of wavelength are required. In this article, we describe the development of light-emitting diode (LED)-based light sources that meet these criteria. These light sources are enabled by a compact LED module that includes an integrated linear driver, heat dissipation technology, and real-time temperature monitoring. Measuring only 27 mm wide by 29 mm high and weighing only 14.7 g, each module provides up to 6,500 lx of white (400–650 nm) light and up to 157 mW of filtered fluorescence excitation light while maintaining an operating temperature ≤ 50°C. We also describe software that can be used to design multimodule light housings and an embedded processor that permits computer control and temperature monitoring. With these tools, we constructed a 76-module, sterilizable, three-wavelength surgical light source capable of providing up to 40,000 lx of white light, 4.0 mW/cm2 of 670 nm near-infrared (NIR) fluorescence excitation light, and 14.0 mW/cm2 of 760 nm NIR fluorescence excitation light over a 15 cm diameter field of view. Using this light source, we demonstrated NIR fluorescence–guided surgery in a large-animal model.

scholarly journals LED Rail Signals: Full Hardware Realization of Apparatus with Independent Intensity by Temperature Changes

Electronics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1291
Author(s):  
Giuseppe Schirripa Schirripa Spagnolo ◽  
Fabio Leccese
Keyword(s):  
Temperature Sensor ◽  
Energy Efficient ◽  
Light Emitting Diode ◽  
Junction Temperature ◽  
Light Emitting ◽  
Temperature Changes ◽  
Wide Range ◽  
International Regulations ◽  
Long Lifetime ◽  
Set Up

Nowadays, signal lights are made using light-emitting diode arrays (LEDs). These devices are extremely energy efficient and have a very long lifetime. Unfortunately, especially for yellow/amber LEDs, the intensity of the light is closely related to the junction temperature. This makes it difficult to design signal lights to be used in naval, road, railway, and aeronautical sectors, capable of fully respecting national and international regulations. Furthermore, the limitations prescribed by the standards must be respected in a wide range of temperature variations. In other words, in the signaling apparatuses, a system that varies the light intensity emitted according to the operating temperature is useful/necessary. In this paper, we propose a simple and effective solution. In order to adjust the intensity of the light emitted by the LEDs, we use an LED identical to those used to emit light as a temperature sensor. The proposed system was created and tested in the laboratory. As the same device as the ones to be controlled is used as the temperature sensor, the system is very stable and easy to set up.

Electrically driven single microribbon based near-infrared exciton-polariton light-emitting diode

CrystEngComm ◽  
2021 ◽  
Author(s):  
Mingming Jiang ◽  
Fupeng Zhang ◽  
Kai Tang ◽  
Peng Wan ◽  
Caixia Kan
Keyword(s):  
High Performance ◽  
Near Infrared ◽  
Light Emitting Diodes ◽  
Light Emitting Diode ◽  
Light Sources ◽  
Coherent Light ◽  
Light Emitting ◽  
Exciton Polaritons ◽  
Laser Devices

Achieving electrically-driven exciton-polaritons has drawn substantial attention toward developing ultralow-threshold coherent light sources, containing polariton laser devices and high-performance light-emitting diodes (LEDs). In this work, we demonstrate an electrically driven...

Reliability of High-Power Light Emitting Diode Attached With Different Thermal Interface Materials

2010 ◽  
Vol 132 (3) ◽  
Author(s):  
Xin Li ◽  
Xu Chen ◽  
Guo-Quan Lu
Keyword(s):  
High Power ◽  
Heat Dissipation ◽  
Light Emitting Diode ◽  
Temperature Cycling ◽  
Fluorescent Light ◽  
Light Sources ◽  
Light Emitting ◽  
Die Attach ◽  
Nanosilver Paste ◽  
Interconnection Material

As a solid electroluminescent source, white light emitting diode (LED) has entered a practical stage and become an alternative to replace incandescent and fluorescent light sources. However, due to the increasing integration and miniaturization of LED chips, heat flux inside the chip is also increasing, which puts the packaging into the position to meet higher requirements of heat dissipation. In this study, a new interconnection material—nanosilver paste is used for the LED chip packaging to pursue a better optical performance, since high thermal conductivity of this material can help improve the efficiency of heat dissipation for the LED chip. The bonding ability of this new die-attach material is evaluated by their bonding strength. Moreover, high-power LED modules connected with nanosilver paste, Sn3Ag0.5Cu solder, and silver epoxy are aged under hygrothermal aging and temperature cycling tests. The performances of these LED modules are tested at different aging time. The results show that LED modules sintered with nanosilver paste have the best performance and stability.

scholarly journals Towards the Optimum Light Source Spectrum

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Andrew Chalmers ◽  
Snjezana Soltic
Keyword(s):  
Light Source ◽  
Large Scale ◽  
Source Spectrum ◽  
Light Sources ◽  
Luminous Efficacy ◽  
Light Emitting ◽  
Spectral Bands ◽  
Good Energy ◽  
Lighting Systems ◽  
Colour Rendering

This paper is concerned with designing light source spectra for optimum luminous efficacy and colour rendering. We demonstrate that it is possible to design light sources that can provide both good colour rendering and high luminous efficacy by combining the outputs of a number of narrowband spectral constituents. Also, the achievable results depend on the numbers and wavelengths of the different spectral bands utilized in the mixture. Practical realization of these concepts has been demonstrated in this pilot study which combines a number of simulations with tests using real LEDs (light emitting diodes). Such sources are capable of providing highly efficient lighting systems with good energy conservation potential. Further research is underway to investigate the practicalities of our proposals in relation to large-scale light source production.

In-Use Photostability Practice and Regulatory Evaluation for Pharmaceutical Products in an Age of Light-Emitting Diode Light Sources

2019 ◽  
Vol 108 (3) ◽  
pp. 1172-1176 ◽  
Author(s):  
Leonardo R. Allain ◽  
Brittany C. Pierce ◽  
W. Peter Wuelfing ◽  
Allen C. Templeton ◽  
Roy Helmy
Keyword(s):  
Light Emitting Diode ◽  
Pharmaceutical Products ◽  
Light Sources ◽  
Light Emitting ◽  
Regulatory Evaluation

Manufacture and Evaluation of Light Emitting Diode Package Substrate Using Flexible Printed Circuit Board

2015 ◽  
Vol 15 (10) ◽  
pp. 7578-7581
Author(s):  
Jung-Kab Park ◽  
Jin-Ha Shin ◽  
Mun-Gi Jung ◽  
Tomabechi Shigehisa ◽  
Hwa-Sun Park ◽  
...  
Keyword(s):  
Thermal Resistance ◽  
Light Emitting Diode ◽  
Thermal Conductance ◽  
Polyimide Film ◽  
Luminous Efficiency ◽  
Circuit Board ◽  
Light Sources ◽  
Thermal Shock Test ◽  
Light Emitting ◽  
Au Electrode

Unlike other light sources such as fluorescent lamps and incandescent bulbs, light-emitting diodes (LED) convert 70∼80% of energy into heat. If the heat produced an LED chip is not effectively released, its luminous efficiency and lifespan are reduced. Therefore, as a method effectively release heat, an LED PKG substrate containing a heat-releasing material with excellent thermal conductance was fabricated, and its thermal resistance and luminous efficiency were analyzed. In this experiment, a thin polyimide film with excellent ductility was used to fabricate the LED PKG substrate. A 35-μm-thick Cu foil with excellent thermal conductance was subjected to high temperature and pressure and attached to both sides of the polyimide film. By electroplating Ag or Au, which has excellent thermal conductance, for us as the electrode and heat-releasing material, LED PKG substrate was fabricated with a thickness of approximately 170 μm. (−40 °C → RT → 120 °C). The results revealed that the LED PKG substrate having a Ag electrode with excellent thermal conductance had an excellent thermal resistance of approximately 4.2 °C/W (Au electrode: 5.6 °C/W). The luminous flux after 100 cycles in the thermal shock test was reduced by approximately 0.09% (Au electrode: 2.77%), indicating that the LED PKG substrate had excellent thermal resistance without any mechanical and material defects in a rapid-temperature-changing environment. The advantages and excellent thermal resistance can be exploited in cellular phones and LCD panels, and heat-releasing problems in thin panels be solved.

scholarly journals Pinhole microLED Array as Point Source Illumination for Miniaturized Lensless Cell Monitoring Systems

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 866 ◽  
Author(s):  
Shinta Mariana ◽  
Gregor Scholz ◽  
Feng Yu ◽  
Agus Budi Dharmawan ◽  
Iqbal Syamsu ◽  
...  
Keyword(s):  
Image Quality ◽  
Light Emitting Diode ◽  
Free Cell ◽  
Monitoring Systems ◽  
Label Free ◽  
Light Emitting ◽  
Non Invasive ◽  
Cell Monitoring ◽  
Blue Spectral Region ◽  
Led Arrays

Pinhole‐shaped light‐emitting diode (LED) arrays with dimension ranging from 100 μm down to 5 μm have been developed as point illumination sources. The proposed microLED arrays, which are based on gallium nitride (GaN) technology and emitting in the blue spectral region (λ = 465 nm), are integrated into a compact lensless holographic microscope for a non‐invasive, label‐free cell sensing and imaging. From the experimental results using single pinhole LEDs having a diameter of 90 μm, the reconstructed images display better resolution and enhanced image quality compared to those captured using a commercial surface‐mount device (SMD)‐based LED.

Optimization and evaluation of a blue light photoinitiating system for textile inkjet printing

2018 ◽  
Vol 89 (10) ◽  
pp. 1964-1974
Author(s):  
Yi Huang ◽  
Guangdong Sun ◽  
Yating Ji ◽  
Dapeng Li ◽  
Qinguo Fan ◽  
...  
Keyword(s):  
Blue Light ◽  
Light Emitting Diode ◽  
Absorption Efficiency ◽  
Polymerization Rate ◽  
Nozzle Clogging ◽  
Light Emitting ◽  
Custom Made ◽  
Light Curing ◽  
Set Up ◽  
Pigment Ink

A blue light curing process was developed to solve the nozzle clogging challenge commonly encountered in conventional textile pigment printing, by using camphorquinone (CQ) and ethyl-4-dimethylaminobenzoate (EDMAB) as a photoinitiator combination and substituting oligomers and monomers for a polymeric binder. High light absorption efficiency was insured by closely matching the spectrum of the photoinitiator with a custom-made blue light light-emitting diode set-up. Kinetic analyses of such a CQ/EDMAB system indicated that the maximum polymerization rate of the monomer was proportional to [PI]0.5 and [I0]0.5, while excessive high photoinitiator concentration (>1 wt%) will decrease the polymerization rate because of the “filter effect.” With optimized blue light curable pigment ink formula and irradiation conditions, the photocurable pigment printed fabrics exhibited uniform and vibrant colors, clear outlines, and excellent wet and dry rubbing fastness of grades 4 and 4–5, respectively.

An intraocular micro light-emitting diode device for endo-illumination during pars plana vitrectomy

2018 ◽  
Vol 29 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Philipp S Koelbl ◽  
Christian Lingenfelder ◽  
Christoph W Spraul ◽  
Juergen Kampmeier ◽  
Frank HJ Koch ◽  
...  
Keyword(s):  
Light Source ◽  
Pars Plana Vitrectomy ◽  
Light Emitting Diode ◽  
Outer Diameter ◽  
Continuous Exposure ◽  
Thermal Hazards ◽  
Light Emitting ◽  
Illumination System ◽  
Single Use ◽  
Pars Plana

Purpose: Development of a new, fiber-free, single-use endo-illuminator for pars plana vitrectomy as a replacement for fiber-based systems with external light sources. The hand-guided intraocularly placed white micro light-emitting diode is evaluated for its illumination properties and potential photochemical and thermal hazards. Methods: A micro light-emitting diode was used to develop a single-use intraocular illumination system. The light-source-on-tip device was implemented in a prototype with 23G trocar compatible outer diameter of 0.6 mm. The experimental testing was performed on porcine eyes. All calculations of possible photochemical and thermal hazards during the application of the intraocular micro light-emitting diode were calculated according to DIN EN ISO 15007–2: 2014. Results: The endo-illuminator generated a homogeneous and bright illumination of the intraocular space. The color impression was physiologic and natural. Contrary to initial apprehension, the possible risk caused by inserting a light-emitting diode into the intraocular vitreous was much smaller when compared to conventional fiber-based illumination systems. The photochemical and thermal hazards allowed a continuous exposure time to the retina of at least 4.7 h. Conclusion: This first intraocular light source showed that a light-emitting diode can be introduced into the eye. The system can be built as single-use illumination system. This light-source-on-tip light-emitting diode–endo-illumination combines a chandelier wide-angle illumination with an adjustable endo-illuminator.

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