scholarly journals Πρωτεωμική ανάλυση βιολογικών δειγμάτων για την ανάδειξη βιοδεικτών του καρκίνου

2011 ◽  
Author(s):  
Θεόδωρος Ρουμελιώτης
Keyword(s):  
Mass Spectrometry ◽  
Blood Serum ◽  
Biological Samples ◽  
State Of The Art ◽  
Genetic Constitution ◽  
Major Public Health Problem ◽  
Pharmacological Response ◽  
Depth Analysis ◽  
Potential Biomarkers

The various forms of cancer is a major public health problem of the modern societies. Several environmental and genetic factors have been recognized to be responsible for the occurrence of cancer and key biological mechanisms involved have been revealed as well. However, early diagnosis, prognosis of progression and the identification of pharmacological therapeutic targets remain partially ineffective fields in clinical practice despite the fact that studies focusing on these areas are increasing rapidly. This is largely due to the complexity and diversity of both the disease and the genetic constitution of individuals and hence of the biological samples. Therefore, the main characteristics that an experimental approach should concentrate in order to provide solutions to such questions should be, holisticity, accuracy, sensitivity, cross-verification and the use of well-characterized biological samples. State of the art proteomics based on mass spectrometry is a useful tool for the first step in this direction which is the identification of potential biomarkers which then should be tested for their sensitivity and specificity using simple and validated biochemical techniques in inter-laboratory assays. This study, presents novel applications of combined state of the art proteomic techniques for the in-depth analysis and characterization of biological specimens such as tissue and blood serum for the determination of potential biomarkers of diagnosis, prognosis and pharmacological response for breast cancer and prostate cancer as well. The analysis of biological samples was based on the principles of multidimensional liquid chromatography combined with tandem mass spectrometry. The study resulted in the qualitative and semi-quantitative determination of a large number of proteins that extensively covers the proteome of tissues from breast biopsies and blood serum compared with the data available in current bibliography. Finally, proteins with differences in expression between the different biological states studied were identified with potential in diagnosis, prognosis and pharmacological response that consist innovative findings amenable to further study.

scholarly journals Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

2021 ◽  
Vol 22 (12) ◽  
pp. 6283
Author(s):  
Jérémy Lamarche ◽  
Luisa Ronga ◽  
Joanna Szpunar ◽  
Ryszard Lobinski
Keyword(s):  
Mass Spectrometry ◽  
State Of The Art ◽  
Animal Health ◽  
Primary Standard ◽  
Selenoprotein P ◽  
Primary Standards ◽  
Analytical Approaches ◽  
Careful Investigation ◽  
Accurate Quantification

Selenoprotein P (SELENOP) is an emerging marker of the nutritional status of selenium and of various diseases, however, its chemical characteristics still need to be investigated and methods for its accurate quantitation improved. SELENOP is unique among selenoproteins, as it contains multiple genetically encoded SeCys residues, whereas all the other characterized selenoproteins contain just one. SELENOP occurs in the form of multiple isoforms, truncated species and post-translationally modified variants which are relatively poorly characterized. The accurate quantification of SELENOP is contingent on the availability of specific primary standards and reference methods. Before recombinant SELENOP becomes available to be used as a primary standard, careful investigation of the characteristics of the SELENOP measured by electrospray MS and strict control of the recoveries at the various steps of the analytical procedures are strongly recommended. This review critically discusses the state-of-the-art of analytical approaches to the characterization and quantification of SELENOP. While immunoassays remain the standard for the determination of human and animal health status, because of their speed and simplicity, mass spectrometry techniques offer many attractive and complementary features that are highlighted and critically evaluated.

scholarly journals Ion Mobility–Mass Spectrometry for Bioanalysis

Separations ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 33
Author(s):  
Xavier Garcia ◽  
Maria del Mar Sabaté ◽  
Jorge Aubets ◽  
Josep Maria Jansat ◽  
Sonia Sentellas
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Biological Samples ◽  
Ion Mobility Mass Spectrometry ◽  
Exogenous Compounds ◽  
Analytical Separation

This paper aims to cover the main strategies based on ion mobility spectrometry (IMS) for the analysis of biological samples. The determination of endogenous and exogenous compounds in such samples is important for the understanding of the health status of individuals. For this reason, the development of new approaches that can be complementary to the ones already established (mainly based on liquid chromatography coupled to mass spectrometry) is welcomed. In this regard, ion mobility spectrometry has appeared in the analytical scenario as a powerful technique for the separation and characterization of compounds based on their mobility. IMS has been used in several areas taking advantage of its orthogonality with other analytical separation techniques, such as liquid chromatography, gas chromatography, capillary electrophoresis, or supercritical fluid chromatography. Bioanalysis is not one of the areas where IMS has been more extensively applied. However, over the last years, the interest in using this approach for the analysis of biological samples has clearly increased. This paper introduces the reader to the principles controlling the separation in IMS and reviews recent applications using this technique in the field of bioanalysis.

PSV-4 Determination of Deoxynivalenol (DON) Content in Biological Samples as an Indicator of DON Intake in Grower-finisher Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 206-207
Author(s):  
Michael O Wellington ◽  
Michael A Bosompem ◽  
Veronika Nagl ◽  
Daniel A Columbus
Keyword(s):  
Mass Spectrometry ◽  
Biological Samples ◽  
High Performance ◽  
Tandem Mass ◽  
Serum Samples ◽  
Blood Samples ◽  
Swine Diets ◽  
Direct Quantification ◽  
Serum And Urine

Abstract Due to difficulties in obtaining consistent and/or reliable measures of deoxynivalenol (DON) in complete swine diets, we investigated whether measuring DON in biological samples could be used as an indicator of DON ingestion in pigs. In this study, graded levels of DON (1, 3, or 5 ppm) were fed to grower-finisher pigs for a period of 77-d. On d 35 and 77 of the study, urine samples were quantitatively collected over a 24-h period and blood samples were collected between 3 – 4 h after the morning meal on each of those days for serum DON analysis. For direct quantification of DON in urine, high-performance liquid chromatography with tandem mass spectrometry was performed. For serum samples, indirect quantification of DON was performed via enzymatic hydrolysis. We observed that DON content in urine increased linearly as intake of DON increased (Fig.1A; P < 0.05). Analysis of DON in serum follow a similar trend, where serum DON content was increased as DON intake increased (Fig.1B; P < 0.05). An average of 30% of DON ingested was recovered as DON in urine over a 24-h period. In summary, there was a linear relationship between DON intake and DON content in both urine and blood serum, therefore, analyzing DON concentration in serum and urine could be used as a tool to estimate for DON exposure in pigs under controlled conditions.

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