scholarly journals Influence of dietary olive paste flour on the performance and oxidative stress in chickens raised in field conditions

2021 ◽  
Vol 72 (3) ◽  
pp. 3239
Author(s):  
E FOTOU ◽  
V MOULASIOTI ◽  
I ANGELIS ◽  
V TSIOURIS ◽  
A PATSIAS ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Muscle Tissue ◽  
Growth Retardation ◽  
Antioxidant Status ◽  
Field Conditions ◽  
Poultry Feed ◽  
Control Group ◽  
Tocopherol Concentration ◽  
Organoleptic Characteristics

Olive oil contains a variety of antioxidants, including vitamin E. Its consumption protects against oxidative stress, which is involved in many pathological conditions, affecting animals’ development and their general welfare. The present study aimed to investigate the effect of olive paste flour (OPF) on the antioxidant status and performance of broiler chickens raised in field conditions. Total of 18.000 broilers was randomly allocated in equal numbers in two poultry houses. The chickens were grouped according to their diet as follows: Control group: chickens fed commercial poultry feed, and OPF group: chickens fed control dietsin the starter period, but they got a supplement of OPF in grower, and finisher 1 and 2periods, respectively. The birds were raised under identical field conditions (ventilation, vaccination, lighting, etc.). Antioxidant status was assessed by measuring the concentration of vitamin E in plasma, as well as the total antioxidant capacity (TAC) in plasma and muscle tissue. According to the statistical analysis of the results, the addition of OPF to chickens’ diet significantly enhanced plasma α-tocopherol concentration (p≤0.05). TAC did not show any significant differences in chicken plasma nor muscle tissue(p>0.05).Feed intake (FI) was higher in OPF group, while Body weight (BW) was lower. Liveability was similar for the two groups. The feed conversion ratio (FCR) was higher, and the European production efficiency factor (EPEF) was lower in broilers of the OPF group compared to those of the control group.Samples of roasted breast from chickens of both groups were used for organoleptic characteristics evaluation. Results showed that samples of the OPF group smelled more intensely and were more tasteful than those of the control group.However, other organoleptic characteristics did not differ. In conclusion, the results demonstrated that although the addition of OPF to chickens’ diet can cause growth retardation, it can significantly increase the plasma α-tocopherol concentration. Further studies are needed to optimize the concentration of OPF in poultry feed in order to avoid growth retardation or even to promote growth in broiler chicks.

scholarly journals Total Oxidant and Antioxidant Capacity in Patients with Transfusion Dependent and Nondependent Beta Thalassemia

Blood ◽  
2015 ◽  
Vol 126 (23) ◽  
pp. 4573-4573
Author(s):  
Zeynep Karakas ◽  
Yasin Yilmaz ◽  
Dolay Damla Celik ◽  
Agageldi Annayev ◽  
Selcan Demirel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Antioxidant Status ◽  
Conflicts Of Interest ◽  
Beta Thalassemia ◽  
Control Group ◽  
Simple Method ◽  
Total Antioxidant

Abstract Background: The patients with β-thalassemia are usually under oxidative stress due to iron overload as a result of ineffective erythropoiesis and repeated transfusions. The endogen (uric acid, indirect bilirubin) and exogenous (ascorbic acid and vitamin E) antioxidants protect the cells from oxidative damage. The evaluation of antioxidant defense system can be easily done by measuring serum total antioxidant capacity. The aim of this study is to investigate the total oxidant (TOC) and antioxidant capacity (TAC) of patients with transfusion dependent (TDT) and nondependent (NTDT) β -thalassemia. Methods: Fifty eight patients (age range 3-59; median age 24 years) with β-thalassemia (16 nontransfusion dependent, 42 transfusion dependent) who were followed-up by Istanbul Medical Faculty Thalassemia Center were enrolled in this study. The sex and age matched 25 healthy subjects were used as control group. The total oxidant and antioxidant capacity were measured by Rel Assay Diagnostics, Total Oxidant Status (TOS) kit and Total Antioxidant Status (TAS) kit. The statistics of results were done by SPSS version 21. Results: 58 patients were included consisting of β thalassemia intermedia (n=16) and β thalassemia major (n=42). The total oxidant capacity was found higher in patients than control group without significant differences. Beside this, the antioxidant capacity of patients was significantly increased (2.75 mmol/l vs 1.94 mmol/l; p=0.001). Within patients group, there was no significant differences in terms of TAC while the TOC level was high in NTDT (2.99 mmol/l vs 1.88 mmol/l; p=0.08). There was no significant relationship between TOC and sex, age (<18 vs >18 years), ferritin and splenectomy status, as the same for TAC. Conclusions: The oxidative damage in patients with β-thalassemia can trigger the morbidity. The measurement of TOC and TAC status stands a useful, rapid, and simple method to evaluate the complex oxidative mechanism of disease. There are still controversial results about antioxidant status in thalassemia patients. The irregular consume of supportive treatment such as zinc, vitamin C, vitamin E and herbal medicine may affect the TOC and TAC status. The results of this study can guide us to use the antioxidants to decrease the oxidative stress. Figure 1. Figure 1. Disclosures No relevant conflicts of interest to declare.

scholarly journals To Evaluate the Effect of Vitamin E Therapy on the Oxidative Stress Markers (Nitric Oxide, SOD, Glutathione Peroxidase) & Vitamin E Levels in Pulmonary Tuberculosis Patients

2020 ◽  
Vol 9 (40) ◽  
pp. 2970-2975
Author(s):  
Rohit John Chaudhary ◽  
Bharti Kwatra Uppal
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Pulmonary Tuberculosis ◽  
Older Age ◽  
Control Group ◽  
Ros Production ◽  
Age Group ◽  
Sod Activity

BACKGROUND Severe oxidative stress has been reported in TB patients because of infection associated with malnutrition and poor immunity. Mycobacteria can induce reactive oxygen species (ROS) production by activating phagocytes, and enhanced ROS production may promote tissue injury and inflammation. We wanted to compare the effect of antioxidant administration in the outcome of ATT treatment between the test and the control group. METHODS This perspective study was conducted in the Departments of Biochemistry and Chest Medicine, CMC & Hospital. Hundred patients (fifty controls and fifty tests) who were diagnosed as pulmonary tuberculosis and started on DOT therapy under RNTCP during this period were included in the study. Each participant in the study was subjected to the following test at the first visit, 2nd month and 6th month follow up (biochemical markers Nitric oxide, SOD, Glutathione Peroxidase and Vitamin E levels). Statistical analysis was done using SPSS version. RESULTS The results were based on four categories (male / female, alcoholic / non-alcoholic, smoker / non-smoker, and younger / older age group). Females had responded better with greater fall in percentage of nitric oxide values (69 %) than males (64.1 %). The mean of SOD activity (277.5 + / - 31.5) was more in smokers than non-smokers (261.3 + / - 36.0) & percentage fall of nitric oxide in smokers (65 %) & non-smokers (67 %). In alcoholics the percentage fall of nitric oxide (68.3 %) was higher with more SOD activity (Mean 278.7 + / - 27.6) than non-alcoholics (Mean 256 + / - 38.0) indicating a positive correlation of smoking & alcoholism with tuberculosis. Younger age group responded better with more fall in the percentage of nitric oxide (67 %) & mean SOD activity (265.8 + / - 30.1) than older age group. CONCLUSIONS Antioxidant supplementation reduces oxidative stress, improves the effectiveness of ATT therapy, and thus helps in improving the outcome in pulmonary tuberculosis. KEY WORDS Pulmonary TB, ATT (Anti-Tubercular Treatment), Antioxidants & Free Radicals

The Antioxidant Efficacy of Wheatgrass (Triticum Aestivum) on Mercuric Chloride (HgCl2) - Induced Oxidative Stress in Rat Model

2021 ◽  
Vol 9 (2) ◽  
pp. 450-464
Author(s):  
Renu Tripathi ◽  
Swati Agarwal ◽  
Syed Ibrahim Rizvi ◽  
Neetu * Mishra
Keyword(s):  
Oxidative Stress ◽  
Mercuric Chloride ◽  
Rat Model ◽  
Antioxidant Status ◽  
Protective Efficacy ◽  
Density Lipoprotein ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidant Power ◽  
Group I

Mercury is a harmful toxic pollutant, which has hepato-nephrotoxic, hematotoxic, genotoxic and neurotoxic, effects. The aim of the study was to evaluate the protective efficacy of wheatgrass on mercuric chloride (HgCl2) induced oxidative stress and associated complications in rat model. Albino rats were divided into four groups (three rats per group). Group I normal control group. Group II oxidative stressed group received mercuric chloride (0.5 mg/kg/day). Group III only received wheatgrass extract (100 mg/kg/day), whereas Group IV received wheatgrass (100 mg/kg/day) after one hour, followed by mercuric chloride (0.5 mg/kg/day) for 30 days. The results of the study showed that wheatgrass supplementation significantly decreased the HgCl2 induced elevated oxidative stress parameters Plasma Malondialdehyde (MDA) content, Plasma membrane redox system (PMRS), Advanced oxidation protein products (AOPP), simultaneously elevated lipid profile (Total Cholesterol, Triglycerides, Low-density lipoprotein (LDL), liver enzymes as, Plasma Alkaline phosphatase (ALP), Aspartate aminotransferase (AST), and Alanine aminotransferase (ALT), Serum Urea, and Creatinine levels in rats. In addition, wheatgrass treatment improved the antioxidant status in terms of intracellular Reduced Glutathione (GSH), Ferric reducing antioxidant power (FRAP) and 2, 2- diphenyl -1- picrylhydrazyl (DPPH). Therefore it can be concluded that wheatgrass has great potential to diminish the stress-mediated complications and improve the antioxidant status.

scholarly journals The Effects of Hyperbaric Oxygen at Different Pressures on Oxidative Stress and Antioxidant Status in Rats

Medicina ◽  
2019 ◽  
Vol 55 (5) ◽  
pp. 205 ◽  
Author(s):  
Şefika Körpınar ◽  
Hafize Uzun
Keyword(s):  
Oxidative Stress ◽  
Hyperbaric Oxygen ◽  
Antioxidant Status ◽  
Central Retinal Artery Occlusion ◽  
Retinal Artery Occlusion ◽  
Antioxidant Response ◽  
Control Group ◽  
Albino Rats ◽  
Normobaric Oxygen ◽  
Group I

Background: The optimal use of oxygen at greater than atmospheric pressures in any operational or therapeutic application (hyperbaric oxygen, HBO2) requires awareness of the fact that the beneficial effects of oxygen coexist with toxic effects depending on the pressure and duration of exposure. In this study, we aimed to investigate the effect of HBO2 therapy on oxidative stress and antioxidant status in commonly used protocol for acute HBO2 indications, such as carbon monoxide intoxication, central retinal artery occlusion, crush injury, gas gangrene, and to compare it with normobaric oxygen (NBO2) in healthy rats. Materials and Methods: Fifty-six male, young adult Wistar albino rats were randomly divided into seven groups and named as Group I through Group VII. Plasma malondialdehyde (MDA), superoxide dismutase (SOD), and erythrocyte glutathione (GSH) levels in control group were compared to the levels in other groups. Results: The increases in MDA levels and the decrease in SOD activities were statistically significant in HBO2 groups at the end of the first 24 h when compared to the control group, and the significant decrease in erythrocyte GSH level was only at 2.4 atmospheres absolute. Conclusions: The present study showed that pressure and frequency of exposure are important factors to consider when investigating HBO2-induced oxidative stress and antioxidant response.

scholarly journals Oxidative Stress and Antioxidant Status in Patients of acute Myocardial Infarction before and after Regular Treatment

2015 ◽  
Vol 10 (2) ◽  
pp. 60-65 ◽  
Author(s):  
Tahmina Bashar ◽  
Nargis Akhter
Keyword(s):  
Oxidative Stress ◽  
Risk Factors ◽  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Vitamin E ◽  
Antioxidant Status ◽  
Plasma Vitamin ◽  
Regular Treatment ◽  
Before And After ◽  
Plasma Vitamin E

In acute myocardial infarction (AMI), generation of free radicals contribute to its pathogenesis. We evaluated the extent of oxidative stress and its amelioration in AMI patients after regular treatment and assessed its association with risk factors like dyslipidemia and diabetes mellitus (DM). Plasma MDA level increased (p<0.001) and erythrocyte GSH and plasma vitamin E levels decreased (p<0.001) in AMI patients. On the 5th day of regular treatment MDA level reduced (p<0.001) and erythrocyte GSH and vitamin E levels increased (p<0.001). The rise in MDA and fall in vitamin E levels were significant (p<0.001) in patients with both dyslipidemia and DM in comparison to patients without dyslipidemia or DM. It is concluded that an imbalance exists between oxidant and antioxidant molecules in AMI patients which shifts towards oxidative side and treatment restores this balance. There may be some association between oxidative stress in AMI and risk factors like dyslipidemia and diabetes mellitus.University Heart Journal Vol. 10, No. 2, July 2014; 60-65

scholarly journals Increased Oxidative Stress in Prepubertal Children Born Small for Gestational Age

2007 ◽  
Vol 92 (4) ◽  
pp. 1372-1378 ◽  
Author(s):  
Angelika Mohn ◽  
Valentina Chiavaroli ◽  
Marina Cerruto ◽  
Annalisa Blasetti ◽  
Cosimo Giannini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Gestational Age ◽  
Small For Gestational Age ◽  
Antioxidant Status ◽  
Lag Phase ◽  
Model Assessment ◽  
Homeostasis Model Assessment ◽  
Prepubertal Children ◽  
Increased Risk

Abstract Context: Low birth weight is associated with an increased risk of metabolic and cardiovascular diseases in adulthood. The development of insulin resistance (IR) seems to play a pivotal role; no data on the oxidant-antioxidant status are available in this risk group. Objective: This study is an assessment of oxidant-antioxidant status in prepubertal children born small for gestational age (SGA) in comparison to healthy controls and the relationship to IR. Design: This cross-sectional study compares indexes of IR and oxidant-antioxidant status in three different groups (SGA+, SGA−, controls), with analysis by post hoc and Pearson correlation. Setting: The study was conducted in the Academic Department of Pediatrics. Participants: A total of 19 SGA+ and 16 SGA− children were compared with 13 controls. Intervention: No intervention was used. Main Outcome Measures: Indexes of IR (glucose to insulin ratio, homeostasis model assessment of IR) were evaluated, and markers of oxidative stress (lag phase, malonildialdehyde, vitamin E) were measured. Results: Homeostasis model assessment of IR was significantly higher in SGA+ than SGA− children (1.32 ± 0.9 vs. 0.69 ± 0.47; P = 0.03) and controls (0.71 ± 0.37; P = 0.04). Glucose to insulin ratio was significantly lower in SGA+ than SGA− children (12.41 ± 5.01 vs. 26.54 ± 17.18; P = 0.02) and controls (26.96 ± 20.70; P = 0.04). Lag phase was significantly shorter in SGA+ than SGA− children (24.3 ± 4.38 vs. 35.59 ± 11.29 min; P = 0.003) and controls (45.28 ± 7.69 min; P = 0.0001) and in SGA− than controls (P = 0.01). Malonildialdehyde was significantly higher in SGA+ than SGA− children (0.79 ± 0.3 vs. 0.6 ± 0.1 nmol/mg; P = 0.03) and controls (0.36 ± 0.04 nmol/mg; P = 0.0001) and in SGA− children than controls (P = 0.02). Vitamin E was significantly reduced in SGA+ children than controls (27.54 ± 7.9 vs. 43.23 ± 11.32 μmol/liter; P = 0.002). Conclusion: Oxidative stress is present in both SGA+ and SGA− children, with a continuous alteration in relation to IR. Therefore, catch-up growth might exert the greatest influence in the development of future diseases.

Effects of xylazine-ketamine, xylazine-propofol and xylazine-ketamine-propofol administration on free radical generation and blood gases in sheep

2018 ◽  
Author(s):  
E. Gokalp ◽  
S. Gurgoze ◽  
S. Altan
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Blood Gases ◽  
Control Group ◽  
Intravenous Route ◽  
Blood Gas ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Significant Difference ◽  
Radical Generation

This study was aimed at investigating the effects of xylazine-ketamine, xylazine-propofol and xylazine-ketamine-propofol combinations on oxidative stress, antioxidant capacity and blood gases in sheep. Excluding the control animals, the sheep included in Groups 1, 2 and 3 were administered with combinations of xylazine-ketamine, xylazine-propofol and xylazine-ketamine-propofol, respectively, by intravenous route. The comparison of the three treatment groups with the control group showed that no significant difference existed for TAS, TOS, MDA and CAT levels. The evaluation of blood gas and electrolyte levels demonstrated a significant decrease in PvO2, cSO2, Na, and Ca levels, and a significant increase in glucose levels. In result, this study showed that the three anaesthetic combinations tested did not have any adverse effect on the oxidant/antioxidant status, but caused significant alterations in blood gas levels.

Biochemical effects of lead exposure on oxidative stress and antioxidant status of battery manufacturing workers of Western Maharashtra, India

2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Ganesh Haribhau Ghanwat ◽  
Arun Jalindar Patil ◽  
Jyotsna A. Patil ◽  
Mandakini S. Kshirsagar ◽  
Ajit Sontakke ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lead Exposure ◽  
Antioxidant Status ◽  
Lipid Peroxide ◽  
Blood Lead ◽  
Control Group ◽  
Oxidative Stress Parameter ◽  
Control Subjects ◽  
Antioxidant Parameters ◽  
Current Scenario

AbstractLead induces oxidative stress and alters the antioxidant status of population exposed to high lead levels, i.e. battery manufacturing workers. The aim of this study was to know the current scenario of blood lead (PbB) levels and their effect on the oxidative stress parameter, i.e. serum lipid peroxide (LP), and antioxidant parameters, such as red blood cell (RBC)-superoxide dismutase (SOD), RBC-catalase (CAT), plasma ceruloplasmin (CP), and serum nitrite, of battery manufacturing workers.Forty-three battery manufacturing workers from Western Maharashtra, India, with ages between 19 and 42 years, were selected as study group and compared with 38 age-matched, healthy male subjects (control group). From both group subjects, 10 mL of blood sample was drawn by puncturing the antecubital vein, and PbB, serum LP, RBC-SOD, RBC-CAT, plasma CP, and serum nitrite were estimated using standard methods.The PbB levels of the battery manufacturing workers were significantly higher (p<0.001, 1050%) as compared with the control subjects. The serum LP levels were significantly increased (p<0.001, 96.86%); all antioxidant status parameters such as RBC-SOD (p<0.001, –26.32%), RBC-CAT (p<0.001, –51.57%), and plasma CP (p<0.001, –35.13%) were significantly decreased; and serum nitrite levels (p<0.001, 154%) were significantly increased in the battery manufacturing workers as compared with the control subjects.Despite modern techniques used to reduce lead exposure in battery manufacturing workers, PbB levels remain high, inducing oxidative stress and altering the antioxidant status of battery manufacturing workers.

scholarly journals Comparison of Oxidant-Antioxidant Status in Patients with Vitiligo and Healthy Population

2015 ◽  
Vol 12 (2) ◽  
pp. 132-136 ◽  
Author(s):  
S Agrawal ◽  
A Kumar ◽  
TK Dhali ◽  
SK Majhi
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Vitamin E ◽  
Vitamin C ◽  
Control Group ◽  
Healthy Population ◽  
Significant Difference ◽  
Destruction Of Melanocytes ◽  
And Control

Background Vitiligo is a well-recognized pigmentary disorder of the skin and /or mucous membrane characterized by circumscribed ivory or chalky white macules devoid of identifiable melanocytes. The pathogenesis of vitiligo is complex and still not well understood. According to autocytotoxic hypothesis, oxidative stress has been suggested to be the initial pathogenic event in melanocyte degeneration. The role of free radicals and oxidative damage in the pathophysiology of vitiligo has been documented in recent studies.Objective To evaluate the role of oxidative stress in patients with vitiligo and of healthy controls by measuring levels of the oxidant malondialdehyde (MDA) and antioxidants vitamin C and vitamin E in serum and catalase (CAT) in erythrocytes.Method A total of 80 clinically diagnosed cases of vitiligo and 80 control subjects were included in the study to assess the activity of MDA, vitamin C and vitamin E in serum and CAT in erythrocytes of patients and controls by using the spectrophotometric assay.Result There was statistically significant increase in the levels of MDA in patients with vitiligo compared to the control group (p<0.001). No significant difference was found in the levels of vitamin C (p=0.411) and vitamin E (p=0.771) between the patients with vitiligo and control group. The levels of CAT in the vitiligo patients were found to be significantly lower than those of controls (p<0.001).Conclusion Increased oxidative stress and decreased catalase have been observed in vitiligo patients and the data suggesting that the free radicals may be involved in the destruction of melanocytes or dysregulation of melanogenesis.Kathmandu University Medical Journal Vol.12(2) 2014: 132-136

scholarly journals Study of antioxidant status in malaria patients

2017 ◽  
Vol 5 (4) ◽  
pp. 1649
Author(s):  
Amit G. Tyagi ◽  
Rupal A. Tyagi ◽  
Prema Ram Choudhary ◽  
Jaidev Singh Shekhawat
Keyword(s):  
Oxidative Stress ◽  
Biochemical Markers ◽  
Antioxidant Status ◽  
Therapeutic Potential ◽  
Control Group ◽  
Oxygen Species ◽  
Medical College ◽  
Follow Up Study ◽  
The Mean

Background: Oxidative stress plays an important role in the development of metabolic changes in malaria patients. During infection RBCs are exposed to continual oxidative stress. The univalent reduction of oxygen results in a series of cytotoxic oxygen species such as O2-, H2O2, OH•. Objective was to determine the level of oxidative stress in patients suffering from malaria.Methods: The present study was conducted on 551 malaria patients and 211 age-sex matched controls, in department of Biochemistry, C U Shah Medical College, Surendranagar, Gujarat from April 2012 to May 2013.  In stage-I, day-1 malaria patient’s v/s control group, In stage-II, day-3 v/s day-1 after anti-malarial treatment and in Stage-III day-3 v/s day-1 after anti-malarial + antioxidant treatment.Results: The mean erythrocytic activity of SOD, CAT, GST were decreased (0.71±0.25EU, 9.9±2.4μmol/sec, and 11.7±3.9 U/gmHb% respectively), mean level of GSH and MDA were increased (42.1±6.06gm/Hb%, 10.9±2.83 respectively) significantly (P<0.001) as compared to control group. In the follow up study with anti-malarial treatment the mean levels of erythrocytic GSH and MDA (28.7±7.54gm/Hb% and 8.08±1.95nM/L) decreased significantly (P<0.001 and P<0.01 respectively), whereas mean activity of erythrocytic enzymes like SOD, CAT and GST (0.99±0.15 EU, 15.8±2.68μmol/sec and 22.5±5U/gmHb%) were increased significantly (P<0.001) as compared to day-1.Conclusions: Erythrocytic antioxidant enzymes, GSH and MDA may be considered to be reliable biochemical markers for diagnostic and therapeutic potential in malaria.

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