scholarly journals Evaluation of antibacterial activity of the brown Seaweed Turbinaria ornata (Turner) J. Agardh from Egypt

2016 ◽  
Vol 4 (8) ◽  
pp. 603-607 ◽  
Author(s):  
Fatma Mohamed Elnabawy Ward ◽  
◽  
Mohamed Ali Deyab ◽  
Author(s):  
Thuy T. T. Thanh ◽  
Thu T. M. Quach ◽  
Van T. T. Tran ◽  
Thanh V. Nguyen ◽  
Shiho Suzuki ◽  
...  

2021 ◽  
Vol 17 ◽  
Author(s):  
Bambang Susilo ◽  
Abd. Rohim ◽  
Midia Lestari W. H.

Background: S. cristaefolium is the brown seaweed extracted using the serial technique with different solvents. Methods: S. cristaefolium powder (50 mesh) was extracted with hexane, ethyl acetate, and methanol respectively. The S. cristaefolium powder residue had been dried before being re-extracted with the next different solvents. Three serial extracts were obtained and named as the 1-stage extract, 2-stage extract, and 3-stage extract. Besides, a single-step extract (extraction using only methanol) was also produced to compare with three serial extracts in antibacterial activity tests (against E. coli and S. aureus). The three serial extracts were detected their antibacterial compounds using GC-MS, LC-HRMS, and FT-IR. Results: The 3-stage extract had the highest extraction yield. On S. aureus, the inhibition zone in all extracts was not significantly different. On E.coli, the highest inhibition zone (5.42±0.14 mm) was the 3-stage extract, indeed it is higher than both antibiotic and a single-step extract. Phenol, 9-Tricosene(Z)-, palmitic acid, and oleamide were contained in all extracts. Other antibacterial compound types, both the 1-stage and 2-stage extracts contained 8 types whilst the 3-stage extract contained the most types (12 types). Particularly, hexyl cinnamic aldehyde and betaine were detected only in the 3-stage extract with the dominant area. The carboxylic acid groups were detected in all extracts to confirm the fatty acid structure. Several cinnamic aldehyde groups were detected only in the 3-stage extract. Conclusions: Thus, the extraction technique serially could produce the 3-stage extract which has the strongest antibacterial activity and the richest antibacterial compounds.


2018 ◽  
Vol 5 (3) ◽  
pp. 035403 ◽  
Author(s):  
R R Remya ◽  
S R Radhika Rajasree ◽  
T Y Suman ◽  
L Aranganathan ◽  
S Gayathri ◽  
...  

Alginates extracted from brown seaweed have a variety of prospective applications such as thickeners, stabilizers, or restructuring agents. Due to its properties as a natural polysaccharide, alginate is very potential to be used as edible films for food packaging purposes. Edible films are developed for food protection being excellent barriers to gases but not to moisture. Incorporation of antimicrobial agents into edible film formulation can extend product shelf life and reduce the risk of pathogenic bacterial growth on food. Therefore, this study was aimed to develop an alginate based antibacterial edible film. Antibacterial agent extracted from lemongrass leaves using ethanol was employed. The study was conducted by varying the addition levels of lemongrass ethanol extract to the alginate based edible films, i.e. 0.5%, 1.0% and 1.5%. Before being added to the alginate based edible films, the lemongrass ethanol extract was investigated for its antibacterial activity. The edible films obtained were analyzed in terms of physical, mechanical and chemical and microbiological parameters, including thickness, water vapor transmission rate (WVTR), brightness, tensile strength, elongation, moisture content, water solubility and antibacterial activity. Results showed that the higher addition levels of lemongrass ethanol extract tended to produce alginate based edible films with lower WVTR and brightness value as well as higher elongation, moisture content and water solubility. Edible film added with lemongrass ethanol extract resulted in this study demonstrated antibacterial activity against Staphylococcus aureus. The addition of lemongrass alcohol extract at 0.5% was considered as a recommended concentration level for producing alginate based antibacterial edible films.


Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2355
Author(s):  
Allison Leyton ◽  
Michael Araya ◽  
Fadia Tala ◽  
Liset Flores ◽  
María Elena Lienqueo ◽  
...  

Seaweed processing generates liquid fraction residual that could be used as a low-cost nutrient source for microbial production of metabolites. The Rhodotorula strain is able to produce antimicrobial compounds known as sophorolipids. Our aim was to evaluate sophorolipid production, with antibacterial activity, by marine Rhodotorula rubra using liquid fraction residual (LFR) from the brown seaweed Macrocystis pyrifera as the nutrient source. LFR having a composition of 32% w/w carbohydrate, 1% w/w lipids, 15% w/w protein and 52% w/w ash. The best culture condition for sophorolipid production was LFR 40% v/v, without yeast extract, artificial seawater 80% v/v at 15 °C by 3 growth days, with the antibacterial activity of 24.4 ± 3.1 % on Escherichia coli and 21.1 ± 3.8 % on Staphylococcus aureus. It was possible to identify mono-acetylated acidic and methyl ester acidic sophorolipid. These compounds possess potential as pathogen controllers for application in the food industry.


2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Niken - Dharmayanti ◽  
ARMA ANTI ◽  
RESMI RUMENTA SIREGAR ◽  
YULIATI H. SIPAHUTAR ◽  
AEF PERMADI ◽  
...  

Abstract. Dharmayanti N, Anti A, Siregar RR, Sipahutar Y, Permadi A, Siregar AN, Salampessy RB, Sujuliyanti, Nurbani SZ, Purnamasari HB. 2021. Title. Biodiversitas 22: 373-378. Brown seaweeds have the potential to produce bioactive compounds. Bacteria associated with seaweeds are involved in the production of metabolites. Microbes may be present as a living symbiotic in association with other algae as epiphytes or endophytes. In this study, bacteria isolated from brown seaweed (Turbinaria conoides) were tested for antibacterial activity. A total of 14 bacteria were isolated, of which 6 were isolated from external tissue, while 8 from internal tissue. Results of an antagonistic test revealed that 7 isolates showed inhibitory activity against Staphylococcus aureus and only 1 isolate showed the inhibition against both S. aureus and Escherichia coli. Phenotypic and genotypic analysis showed that the symbiont bacteria was Lactobacillus plantarum.


PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 351
Author(s):  
Cicilia Kosasi ◽  
Widya A. Lolo ◽  
Sri Sudewi

ABSTRACT Algae are a place to live for various microorganisms’ symbiosis with them, and some of them were known to be used as antibacterial. This study aims to determine the antibacterial activity of associated bacteria isolates from Turbinaria ornata algae obtained from the Bay of Manado against Staphylococcus aureus and Escherichia coli bacteria. In the course of testing the isolation and purification of symbiotic bacteria from Turbinaria ornata algae, then antibacterial activity was testing using agar diffusion method, then gram staining and biochemical tests were carried out including motility test,  tets, carbohydrate fermentation test, citrate test, lysine test, indole test and catalase test. The result showed  that there were 3 bacterial isolates, which had antibacterial activity in medium category against S.aureus and E.coli bacteria. Based on the results of biochemical identification, the isolates that have the greatest inhibitory power, they were thought to belong to genus Bacillus and the genus Yersinia.Keywords : Antibacterial, Symbionic bacteria, Turbinaria ornata (Turner) J.   Agardh. ABSTRAK Alga merupakan tempat hidup berbagai mikroorganisme yang bersimbiosis dengannya, dan beberapa dari spesies alga diketahui sering dimanfaatkan salah satunya  sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari isolat bakteri yang berasosiasi dengan alga Turbinaria ornata yang diperoleh dari Teluk Manado terhadap bakteri  Staphylococcus aureus dan Escherichia coli. Dalam pengujian dilakukan isolasi dan purfifikasi bakteri simbion dari alga Turbinari ornata, kemudian dilakukan pengujian aktivitas antibakteri menggunakan metode difusi agar, selanjutnya silakukan pewarnaan gram dan uji biokimia meliputi uji motilitas, uji H2S, uji fermentasi karbohidrat, uji sitrat, uji lisin, uji indol dan uji katalase. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri yang mempunyai daya aktivitas antibakteri dalam kategori sedang terhadap bakteri S. aureus dan E. coli. Berdasarkan hasil identifikasi secara biokimia isolat yang mempunyai daya hambat paling besar diduga termasuk dalam genus Bacillus dan genus Yersinia. Kata kunci : Antibakteri, Bakteri simbion, Turbinaria ornata (Turner) J. Agardh.


2017 ◽  
Vol 29 (2) ◽  
pp. 200-214 ◽  
Author(s):  
M. Janarthanan ◽  
M. Senthil Kumar

Purpose The healthcare and hygiene textiles are gaining more importance for their eco-friendly and effective antimicrobial properties that have become essential to safeguard human beings from harmful microorganisms. The fabrics finished with chemical-based antimicrobial agents lead to environmental issues and are harmful to human beings. The paper aims to discuss these issues. Design/methodology/approach The present investigation is to develop a fabric with antioxidant and antimicrobial properties using the extracts of brown algae. Antimicrobial property has been imparted to the cotton fabric using microcapsules of brown seaweed extracts using the pad-dry-cure method. The presence of bioactive compounds and antioxidant activities of brown algae extracts was evaluated using gas chromatography-mass spectrometry and 2, 2-diphenyl-1-picrylhydrazyl radical scavenging technique, respectively. The total phenolic content of the seaweed extract was determined by the Folin-Ciocalteu method. The minimum bactericidal concentration and minimum inhibitory concentration methods were used to determine the antibacterial activity of the bacterial reduction percentage and parallel streak methods were used evaluate the antibacterial activity of seaweed-treated fabrics. Findings The methanol fraction of the treated fabric had the highest antioxidant activity (42.5+1.21 per cent), because the higher phenolic content traps the reactive oxygen species and develops the cells present in the skin. The results show that the lower inhibition (250 µg/mL) and bactericidal concentrations (1,000 µg/mL) possess higher antibacterial activity. The results also show that the treated fabric possess higher bacterial reduction of 96 per cent and higher zone of inhibition against Escherichia Coli and Staphylococcus Aureus which was about 35 mm and 40 mm. The air permeability, bending length and the wicking behaviour of the treated fabric were slightly reduced, but it has good bursting strength compared with the untreated fabric. Originality/value Such treated fabric is used for making wound dressing, surgical gowns, antibacterial socks and gauze bandage products in healthcare and hygiene textiles.


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