ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN ALGA Turbinaria ornata (Turner) J. Agardh SERTA IDENTIFIKASI SECARA BIOKIMIA

ABSTRACT Algae are a place to live for various microorganisms’ symbiosis with them, and some of them were known to be used as antibacterial. This study aims to determine the antibacterial activity of associated bacteria isolates from Turbinaria ornata algae obtained from the Bay of Manado against Staphylococcus aureus and Escherichia coli bacteria. In the course of testing the isolation and purification of symbiotic bacteria from Turbinaria ornata algae, then antibacterial activity was testing using agar diffusion method, then gram staining and biochemical tests were carried out including motility test, tets, carbohydrate fermentation test, citrate test, lysine test, indole test and catalase test. The result showed that there were 3 bacterial isolates, which had antibacterial activity in medium category against S.aureus and E.coli bacteria. Based on the results of biochemical identification, the isolates that have the greatest inhibitory power, they were thought to belong to genus Bacillus and the genus Yersinia.Keywords : Antibacterial, Symbionic bacteria, Turbinaria ornata (Turner) J. Agardh. ABSTRAK Alga merupakan tempat hidup berbagai mikroorganisme yang bersimbiosis dengannya, dan beberapa dari spesies alga diketahui sering dimanfaatkan salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari isolat bakteri yang berasosiasi dengan alga Turbinaria ornata yang diperoleh dari Teluk Manado terhadap bakteri Staphylococcus aureus dan Escherichia coli. Dalam pengujian dilakukan isolasi dan purfifikasi bakteri simbion dari alga Turbinari ornata, kemudian dilakukan pengujian aktivitas antibakteri menggunakan metode difusi agar, selanjutnya silakukan pewarnaan gram dan uji biokimia meliputi uji motilitas, uji H2S, uji fermentasi karbohidrat, uji sitrat, uji lisin, uji indol dan uji katalase. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri yang mempunyai daya aktivitas antibakteri dalam kategori sedang terhadap bakteri S. aureus dan E. coli. Berdasarkan hasil identifikasi secara biokimia isolat yang mempunyai daya hambat paling besar diduga termasuk dalam genus Bacillus dan genus Yersinia. Kata kunci : Antibakteri, Bakteri simbion, Turbinaria ornata (Turner) J. Agardh.

Download Full-text

AKTIVITAS ANTIBAKTERI FRAKSI ALGA Turbinaria ornata (Turner) J. Agardh YANG DIPEROLEH DARI PERAIRAN TELUK MANADO

PHARMACON ◽

10.35799/pha.8.2019.29298 ◽

2019 ◽

Vol 8 (2) ◽

pp. 325

Author(s):

Nadia Adam ◽

Widya A. Lolo ◽

Sri Sudewi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Raw Materials ◽

Diffusion Method ◽

Marine Biota ◽

Turbinaria Ornata ◽

E Coli ◽

Escherichia Coli Bacteria

ABSTRACT Marine biota such as algae has been developed to be used as a source of medicinal raw materials in the pharmaceutical industry. Extracs from Turbinaria ornata algae J. Agardh showed antibacterial activity. This study aims to determine the antibacterial activity of the Turbinaria ornata (Turner) J. Agardh fraction obtained from the waters of the Manado Bay against the growth of Staphylococcus aureus and Escherichia coli bacteria. The method used in this study is an experimental laboratory. Turbinaria ornata (Turner) J. Agardh was extracted by maceration method with ethanol 96% solvent followed by fractionation and antibacterial activity testing with diffusion method. Antibacterial activity was determined based on the results of the clear zone measurements formed around the disc paper. The results showed that the methanol at a concentration of 10%, 20%, 30%, fractions of 30% ethyl acetate, and fractions of 20% and 30% n-hexane, have antibacterial activity which is categorized strongly in both bacteria of S. aureus and E. coli, while ethyl acetate fractions with a concentration of 10% and 20% againts S.aureus is in the moderate category. Likewise the antibacterial activity of n-hexane fraction with a concentration of 10% againts E. coli was included in the medium category. Keywords: Turbinaria ornata (Turner) J. Agardh, antibacterial, Staphylococcus aureus, Escherichia coli ABSTRAK Biota laut seperti alga telah banyak dikembangkan untuk digunakan sebagai sumber bahan baku obat dalam industri farmasi. Ekstrak dari alga Turbinaria ornata (Turner) J. Agardh menunjukan adanya aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri fraksi Turbinaria ornata (Turner) J. Agardh yang diperoleh dari perairan Teluk Manado terhadap pertumbuhan bakteri Staphylococus aureus dan Escherichia coli. Metode yang digunakan dalam penelitian ini adalah eksperimental laboratorium. Turbinaria ornata (Turner) J. Agardh diekstraksi dengan metode maserasi dengan pelarut etanol 96% dilanjutkan dengan tahap fraksinasi dan pengujian aktivitas antibakteri dengan metode difusi. Aktivitas antibakteri ditentukan berdasarkan hasil pengukuran zona bening yang terbentuk disekitar kertas cakram. Hasil penelitian menunjukkan bahwa fraksi metanol pada konsentrasi 10%. 20% ,30%, fraksi etil asetat konsentrasi 30%, dan fraksi n-heksana pada konsentrasi 20%, 30% memiliki aktivitas antibakteri yang dikategorikan kuat pada kedua bakteri yaitu S. aureus dan E. coli, sedangkan fraksi etil asetat dengan konsentrasi 10% dan 20% pada bakteri S. aureus termasuk dalam kategori sedang. Begitu pula aktivitas antibakteri fraksi n-heksan dengan konsentrasi 10% terhadap E. coli termasuk dalam kategori sedang. Kata kunci : Turbinaria ornata (Turner) J. Agardh, antibakteri, Staphylococus aureus, Escherichia coli.

Download Full-text

ISOLASI DAN UJI ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN SPONS Phyllospongia lamellose SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽

10.35799/pha.8.2019.29260 ◽

2019 ◽

Vol 8 (1) ◽

pp. 252

Author(s):

Sela S Lempoy ◽

Widya A Lolo ◽

Paulina V. Y. Yamlean

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bioactive Compounds ◽

Pathogenic Bacteria ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Isolation And Purification ◽

Esherichia Coli ◽

Identification Stage

ABSTRACT Sponges are one source of producing bioactive components from the sea. Bacteria Symbiosis with sponges are thought to have the potential to produce bioactive compounds that have been isolated from sponges. One of the potential of bioactive compounds which have been found and developed from sponges was antibacterial. The aim of this study was to determine the antibacterial activity of bacteria associated with Phyllospongia lamellose sponges against pathogenic bacteria namely Staphylococcus aureus and Esherichia coli and then proceed with identification of biochemistry in isolates which showed the greatest inhibitory activity. Three isolates of sponges symbiont bacteria were obtained through the isolation and purification stage which were then followed by testing of antibacterial activity with paper disk diffusion method. The antibacterial test results showed the diameter of the inhibitory zone against Staphylococcus aureus, were: SL1(8.67 mm), SL2 (9.33 mm) and SL3(9.00 mm) categorized as medium. Whereas the inhibiotion zone shown on Esherichia coli bacteria is also classified as medium, were: SL1(9.67 mm), SL2 (9.00 mm) and SL3 (9.33 mm). The three isolates continued to the identification stage biochemically. Each isolation was assumed as follows: Desulfotomaculum (SL1), Brochothrix (SL2) and Sulfidobacillus (SL3). Keyword :Phyllospongia lamellose, isolation, biochemically identification ABSTRAK Spons merupakan salah satu sumber penghasil komponen bioaktif yang berasal dari laut. Bakteri yang bersimbiosis dengan spons diduga memiliki potensi dalam menghasilkan senyawa-senyawa bioaktif yang telah diisolasi dari spons. Potensi senyawa bioaktif yang telah ditemukan dan dikembangkan dari spons salah satunya ialah sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari bakteri yang berasosiasi dengan spons Phyllospongia lamellose terhadap bakteri patogen yakni Staphylococcus aureus dan Escherichia coli dan kemudian dilanjutkan dengan identifikasi secara biokimia pada isolat yang menunjukkan daya hambat terbesar. Diperoleh 3 isolat bakteri simbion spons melalui tahap isolasi dan purifikasi yang kemudian dilanjutkan dengan pengujian aktivitas antibakteri melalui metode difusi kertas cakram. Hasil uji antibakteri menunjukkan diameter zona hambat terhadap bakteri Staphylococcus aureus yaitu: SL1(8.67 mm), SL2(9.33 mm) dan SL3 (9.00 mm) termasuk dalam kategori sedang. Sedangkan zona hambat yang ditunjukkan terhadap bakteri Escherichia coli juga tergolong kategori sedang yaitu: SL1(9.67 mm), SL2(9.00 mm) dan SL3 (9.33 mm). Ketiga isolat dilanjutkan ke tahap identifikasi secara biokimia. Masing-masing isolat diduga sebagai berikut: Desulfotomaculum (SL1), Brochothrix (SL2) dan Sulfidobacillus (SL3). Kata kunci :Phyllospongia lamellose, isolasi, identifikasi biokimia.

Download Full-text

UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽

10.33369/atp.v3i2.10122 ◽

2019 ◽

Vol 3 (2) ◽

Author(s):

Deza Oktasila ◽

Nurhamidah Nurhamidah ◽

Dewi Handayani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Ethanol Extract ◽

Inhibition Zone ◽

Raw Material ◽

Diffusion Method ◽

Citrus Leaves ◽

Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10; and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is 7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

Download Full-text

Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽

10.35799/jm.8.2.2019.23514 ◽

2019 ◽

Vol 8 (2) ◽

pp. 42

Author(s):

Florentin Natalia Melsadalam ◽

Dewa Gede Katja ◽

Meiske S. Sangi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Leaf Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Inhibitory Zone ◽

Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

Download Full-text

AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MENGKUDU (Morinda citrifolia L.)

PHARMACON ◽

10.35799/pha.8.2019.29320 ◽

2019 ◽

Vol 8 (2) ◽

pp. 505

Author(s):

Aprilia Aslah ◽

Widya A. Lolo ◽

Imam Jayanto

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Effect ◽

Morinda Citrifolia ◽

Diffusion Method ◽

Methanol Fraction ◽

Fractionation Method ◽

Liquid Fractionation ◽

Escherichia Coli Bacteria

ABSTRACT Noni is a nutritious plant because it has several ingredients that are important for health. Antibacterial components in noni leaves include glycosides, acubins, saponins and flavonoids. The purpose of this study was to determine the fraction of the extract of noni leaf ethanol whether it has an antibacterial effect and know the class of compounds indentifed as having antibacterial activity after TLC-Bioautografi testing was carried out. The samples were extracted using maceration method with 96% ethanol solvent and fractionation using liquid-liquid fractionation method with methanol, ethyl acetat and n-hexane solvents. Antibacterial activity testing was carried out with concentration variants of 20%, 30% and 40% with agar diffusion method (Kirby and Bauer). The results showed that methanol fraction, ethyl acetat fraction and Noni hexane leaf fraction effectively inhibited Staphylococcus aureus and Escherichia coli bacteria. The largest fraction of inhibiting zone was the concentration of 40% methanol which was categorized as strong. TLC monitoring was carried out using the mobile phase of chloroform : n-hexane (2:1). Antibacterial activity testing carried out by contact bioautography method showed that there were spots on the TLC choromatogram, which produced inhibitory zones. Characteristics of blotches were performed with AlCl3 spotting and it was thought that the spots were flavonoids. Keywords : Noni, Antibacterial, Staphylococcus aureus, Escherichia coli, TLC-Bioautografi. ABSTRAK Mengkudu merupakan tanaman yang berkhasiat karena mempunyai beberapa kandungan senyawa yang penting bagi kesehatan tubuh. Komponen yang berkhasiat antibakteri dalam daun mengkudu antara lain adalah glikosida, acubin, saponin dan flavonoid. Tujuan dari penelitian ini adalah untuk mengetahui fraksi dari esktrak etanol daun mengkudu apakah memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT-Bioautografi. Sampel diekstraksi menggunakan metode maserasi dengan pelarut etanol 96% dan fraksinasi dengan metode fraksinasi cair-cair dengan pelarut metanol, etil asetat dan n-heksan. Pengujian aktivitas antibakteri dilakukan dengan varian konsentrasi 20%, 30% dan 40% dengan metode difusi agar (Kirby dan Bauer). Hasil penelitian menunjukan bahwa fraksi metanol, fraksi etil asetat dan fraksi n-heksan daun Mengkudu efektif menghambat bakteri staphylococcus aureus dan Escherichia coli. fraksi yang paling besar zona hambatnya yaitu fraksi metanol konsentrasi 30% yang dikategorikan kuat, dilakukan pemantauan KLT menggunakan fase gerak kloroform : n-heksan (2:1). Pengujian aktivitas antibakteri dilakukan dengan metode bioautografi kontak hasilnya menunjukkan terdapat bercak pada kromatogram KLT yang menghasilkan zona hambat. Karakteristik bercak dilakukan dengan penampak bercak AlCl3 dan diduga bahwa bercak tersebut Flavonoid. Kata kunci : Mengkudu, Antibakteri, Staphylococcus aureus, Escherichia coli , KLT-Bioautografi

Download Full-text

Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

JKPK (Jurnal Kimia dan Pendidikan Kimia) ◽

10.20961/jkpk.v3i3.22742 ◽

2018 ◽

Vol 3 (3) ◽

pp. 201 ◽

Cited By ~ 1

Author(s):

Suryadi Budi Utomo ◽

Mita Fujiyanti ◽

Warih Puji Lestari ◽

Sri Mulyani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Hexadecyltrimethylammonium Bromide ◽

E Coli ◽

Escherichia Coli Bacteria

This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against Staphylococcus aureus and Escherichia coli bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the S. aureus bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against E. coli.

Download Full-text

AKTIVITAS ANTIBAKTERI INFUSA DAUN LIDAH BUAYA (Aloe vera L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli

Jurnal Sains Natural ◽

10.31938/jsn.v6i1.253 ◽

2019 ◽

Vol 6 (1) ◽

pp. 33

Author(s):

Yuni Widyastuti ◽

Nia Yuliani ◽

I.G.A. Manik Widhyastini

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Aloe Vera ◽

Bacterial Count ◽

Inhibition Zone ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Escherichia Coli Bacteria

Antibacterial Activity of Infused Aloe Vera Leaf (Aloe Vera L) on Growth Of Stapylococcus aureus and Escherichia coliDisinfectants may act as antibacterials. Aloe vera (Aloe vera L) is one of the plants that is often used by the community as a medicinal plant that has many active compounds such as lignin, saponin and anthraquinone substances that act as antibacterial. This study aims to determine the antibacterial activity of Aloe vera leaf extracted with infusa method on the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is to see the reduction / reduction of bacterial count (% reduction) after 30 second and 60 second with disc diffusion method. The results showed decrease or decrease of bacteria count (% reduction) to Escherichia coli bacteria found in aloe leaf skin infected by 28,2% after 60 seconds. While decrease or decrease of bacteria amount (% reduction) to Staphyloccocus uareus bacteria infusa mixture of meat and skin of Aloe vera leaves of 27.5% after 60 seconds. Antibacterial test of aloe vera leaf aloe (Aloe veraL) of 100% concentration by disc diffusion method did not show any inhibition zone around cultures of test bacteria.Keywords: Aloe vera, antibacterial activity, Staphylococcus aureus, and Escherichia coli, Infusa.ABSTRAKDesinfektan dapat berfungsi sebagai antibakteri. Lidah buaya (Aloe vera L) merupakan salah satu tanaman yang sering digunakan masyarakat sebagai tanaman obat yang memiliki banyak senyawa aktif seperti lignin, saponin dan antrakuinon yaitu zat yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun lidah buaya (Aloe vera L) yang diekstrak dengan metode infusa terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Metode penelitian yaitu dengan melihat penurunan/pengurangan jumlah bakteri (% reduksi) setelah waktu 30 detik dan 60 detik dengan metode difusi cakram. Hasil penelitian menunjukkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Escherichia coli terdapat pada infusa kulit daun lidah buaya yaitu sebesar 28,2 % setelah waktu 60 detik.Sedangkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Staphyloccocus uareus terdapat pada infusa campuran daging dan kulit daun lidah buaya yaitu sebesar 27,5 % setelah waktu 60 detik. Uji antibakteri infusa daun lidah buaya (Aloe veraL) konsentrasi 100% dengan metode difusi cakram tidak menunjukkan adanya zona penghambatan disekitar biakan bakteri uji.Kata kunci : Lidah buaya, Aktivitas antibakteri, Staphylococcus aureus, dan Escherichia coli, Infusa.

Download Full-text

Identification and antibiogram study of bacterial species isolated from milk samples of different locations in Bangladesh

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v1i3.26462 ◽

2016 ◽

Vol 1 (3) ◽

pp. 457-462 ◽

Cited By ~ 4

Author(s):

Md Nuruzzaman Munsi ◽

Nathu Ram Sarker ◽

Razia Khatun ◽

Mohammed Khorshed Alam

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pathogenic Bacteria ◽

Bacterial Species ◽

Salmonella Typhi ◽

Diffusion Method ◽

Biochemical Tests ◽

Public Health Importance ◽

Milk Samples ◽

First Choice

Cows milk containing pathogenic bacteria is an important threat to the consumers. The objectives of the present study were to identify the bacterial agents of public health importance in milk samples (n=35) of different locations and to determine their sensitivity to different antibiotics. The milk samples were collected and transported aseptically and subsequently allowed for culture in bacteriological media, Grams staining and biochemical tests for the identification of bacterial species. The bacteria identified were Staphylococcus aureus, Escherichia coli and Salmonella typhi, and their prevalence, in case of vendor milk specimens (n=28), were 96.43%, 53.57% and 35.71% respectively, and of brand milk specimens (n=7), were 42.86 %, 28.57% and 0%, respectively. This suggests that cautionary measures should be taken for quality milk production and consumption. The antibiotic sensitivity test was done by disc diffusion method and the average inhibition zones, in case of Staphylococcus aureus, were 32 mm for oxytetracycline, 26 mm for amoxicillin, 35 mm for ciprofloxacin, 27 mm for cefotaxime, 30 mm for ceftriaxone, 30 mm for azithromycin, and 26 mm for erythromycin; in case of Escherichia coli, were 5 mm for oxytetracycline, 9 mm for amoxicillin, 22 mm for ciprofloxacin, 30 mm for cefotaxime, 31 mm for ceftriaxone, 15 mm for azithromycin, and 0 mm for erythromycin; in case of Salmonella typhi., were 25 mm for oxytetracycline, 24 mm for amoxicillin, 38 mm for ciprofloxacin, 31 mm for cefotaxime, 34 mm for ceftriaxone, 24 mm for azithromycin, and 0 mm for erythromycin. Therefore, ciprofloxacin and ceftriaxone may be the antibiotics of first choice, and cefotaxime and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.Asian J. Med. Biol. Res. December 2015, 1(3): 457-462

Download Full-text

Uji Bioaktivitas Antibakteri Senyawa murni dari Jamur Endofit Sporothrix sp Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Dinamika Lingkungan Indonesia ◽

10.31258/dli.6.1.p.37-44 ◽

2019 ◽

Vol 6 (1) ◽

pp. 37

Author(s):

Dewi Yudiana Shinta ◽

Yusmarini Yusmarini ◽

Herix Sonata MS ◽

Hilwan Yuda Teruna ◽

Saryono Saryono

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Endophytic Fungi ◽

Positive Control ◽

Significant Inhibition ◽

New Drugs ◽

Diffusion Method ◽

Significant Difference ◽

Pure Compounds ◽

Escherichia Coli Bacteria

Modern medicines that are developing now come from active ingredients isolated from plants that require large amounts of plants. The development of new drugs from endophytic fungi found obstacles in the amount of pure compounds produced. Therefore further research is needed by using endophytic fungi as a new antimicrobial producer. This study aims to see the ability or activity of pure compounds produced by Sporothrix sp endophytic fungi from Dahlia tuber (Dahlia variabilis). Test the activity of pure compounds produced by Sporothrix sp. Endophytic fungi on E. coli and Staphylococcus aureus determined by disc diffusion method. With doses of 10, 30 and 50μg/disk. In Escherichia coli bacteria doses 10 and 50μg/disk gave significant inhibition of pure compounds from isolation compared to the positive control of ciprofloxacin, which was marked by a statistically significant test result (p <0.05). In contrast to Staphylococcus aureus there was no significant difference in doses of both doses of 10.30 and 50μg/disk. Determination of pure compounds was carried out by HPLC and Infra Red Spectrophotometry.

Download Full-text

Aktivitas Antibakteri dan Antioksidan Asam Galat dari Kulit Buah Lokal yang Diproduksi dengan Tanase

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.14.1.11300.148-159 ◽

2018 ◽

Vol 14 (1) ◽

pp. 148

Author(s):

Eka Junaidi ◽

Yunita Arian Sani Anwar

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Antibacterial Activity ◽

Gallic Acid ◽

Diffusion Method ◽

Syzygium Cumini ◽

Garcinia Mangostana ◽

Dpph Method ◽

Fruit Waste

Penelitian ini merupakan lanjutan dari penelitian sebelumnya tentang produksi asam galat dari limbah kulit buah lokal di Lombok. Limbah kulit buah lokal yang digunakan yaitu kulit buah kepundung (Baccaurea racemosa Muell.Arg), kulit buah juwet (Syzygium cumini), dan kulit buah manggis (Garcinia mangostana). Pada penelitian ini dilakukan pengujian asam galat yang dihasilkan, untuk mengetahui aktivitas antioksidan dan antibakteri. Pengujian sifat antioksidan dilakukan dengan menggunakan metode DPPH, sedangkan aktivitas antibakteri diuji dengan menggunakan metode difusi agar pada bakteri Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa asam galat dari ketiga kulit buah memiliki aktivitas antioksidan. Asam galat dari kulit buah kepundung, kulit buah juwet dan kulit buah manggis memiliki IC50 masing-masing sebesar 5.95; 5,96; dan 5,92 ppm. Berbeda dengan kemampuan sebagai antioksidan, asam galat resistan terhadap bakteri Escherichia coli dan Staphylococcus aureus yang menunjukkan bahwa asam galat tidak memiliki aktivitas sebagai antibakteri.Antibacterial and antioxidant activity of gallic acid from peel local fruit which produced by tannase. This research is a continuation of the previous research about the gallic acid production from peel fruit local in Lombok. The local fruit waste used are kepundung rind (Baccaurea racemosa Muell.Arg), juwet rind (Syzygium cumini) and mangosteen rind (Garcinia mangostana). In this research, the produced Gallic acid was tested to determine its antioxidant and antibacterial activity. The antioxidant test conducted by DPPH method, meanwhile, the antibacterial activity was performed by agar diffusion method to bacteria Escherichia coli and Staphylococcus aureus. The results show that the produce gallic acid from those three rind provide antioxidant activity. Gallic acid from kepundung rind, juwet rind, and mangosteen rind have IC50 values of 5,95; 5,96; and 5,92, respectively. Gallic acid not showed inhibitory actions against the growth of Escherichia coli and Staphylococcus aureus. This result show that gallic acid from local fruit waste did not provide an antibacterial activity.

Download Full-text