scholarly journals Physiological and Molecular Assessment of Sesbania Root Nodules Bacteria from Different Iraqi Areas for Salt Tolerance

2019 ◽  
Vol 12 (1) ◽  
pp. 391-398
Author(s):  
Rana A. Hameed
Keyword(s):  
Cluster Analysis ◽  
Salt Tolerance ◽  
Root Nodules ◽  
Pcr Amplification ◽  
Antibiotic Sensitivity ◽  
Vital Role ◽  
Strong Reaction ◽  
Rapd Pcr ◽  
Significant Difference ◽  
Yeast Mannitol Agar

Thirty two isolates of aerobic gram-negative bacteria associated with Sesbania sesban grown in different saline Iraqi soils was identified according to morphological and physiological characteristics, cultured on yeast-mannitol agar medium (YEMA) supplemented with different NaCl concentrations. It was indicated that 53.12% of isolates were highly tolerant to salinity, tolerated from 4.0 to 5.0 w/v NaCl. All thirty two Rhizobia isolates performed positive strong reaction to Catalase enzyme except for three were negative to this enzyme. Concerning exo-polysaccharide (EPS) production the isolates displayed a significant difference between them and that salt tolerance isolates gave a high amount of EPS production in compare to the sensitive ones. As for antibiotic sensitivity of Sesbania isolates data revealed that 83% of isolates were highly resistant to Ampicilin at 50 µgml-1, the cluster analysis based on all phenotypical and physiological characters divided the isolates into two major groups, the first group included one isolate Ses10, which was salt moderate tolerant. The second group included the rest of isolates which splits into two subgroups with 6% similarity, the first subgroup comprised all sensitive isolates plus one salt moderate tolerant isolate (Ses9).The assumption that district environmental conditions plays a vital role on field survival of bacteria, give rise to the use of PCR methods to identify Rhizobia. In this study the genetic divergence of fast nodulating bacteria connected with Sesbania in Iraq was examined. A selection of Rhizobia isolates were characterized by RAPD –PCR. Amplification of genomic DNA using three random primers (RAPD) gave various bands, the results revealed that most efficient and highest discrimintory power primer was 35.4% and 37% respectively for primer OPA-10. The cluster analysis based on RAPD-PCR amplification results showed two divergent groups with 15% similarity, the first group included two salt sensitive Ses17 and Ses28, and the second major group comprised all salt moderate and tolerant isolates.

scholarly journals In-Vitro Plant Growth Promotion of Rhizobium Strains Isolated from Lentil Root Nodules under Abiotic Stresses

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1006
Author(s):  
Badreddine Sijilmassi ◽  
Abdelkarim Filali-Maltouf ◽  
Sara Fahde ◽  
Youness Ennahli ◽  
Said Boughribil ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Salt Tolerance ◽  
Plant Growth ◽  
Growth Promotion ◽  
Root Nodules ◽  
Rhizobium Strains ◽  
Drought And Salt Tolerance ◽  
Significant Difference ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting rhizobia are known to improve crop performance by multiple mechanisms. However, the interaction between host plants and Rhizobium strains is highly influenced by growing conditions, e.g., heat, cold, drought, soil salinity, nutrient scarcity, etc. The present study was undertaken to assess the use of Rhizobium as plant growth promoters under abiotic stress conditions. Fifteen Rhizobium strains isolated from lentil root nodules were tested for phosphate solubilization activity (PSA) and phytohormones production under salt and drought conditions. The results showed that 15 Rhizobium strains were significant phosphate solubilizers, and indole acedic acid (IAA) and gibberellic acid (GA3) producers based on least significant difference (LSD) analysis (p ≤ 0.05). The highest rate of PSA was attributed to three strains namely, 1145N5, 1159N11, and 1159N32 with a range of 144.6 to 205.6 P2O5 (µg/mL). The highest IAA production was recorded in the strain 686N5 with 57.68 ± 4.25 µg/mL as compared to 50.8667 ± 1.41 µg/mL and 37.32 ± 12.59 µg/mL for Rhizobium tropici CIAT 899 and Azospirillum brasilense DSM-1690, respectively. Strain 318N2111 produced 329.24 ± 7.84 µg/mL of GA3 as against 259.84 ± 25.55 µg/mL for A. brasilense DSM-1690. R. tropici CIAT 899 showed tolerance to salt (5% NaCl) and drought (ψ = −2.6 MPa) stress, whereas strain 686N5 showed an extremely high level of salt-tolerance (5% NaCl) and moderate level of drought tolerance (ψ = −0.75 MPa). These results indicate different pathways for drought and salt tolerance mechanisms. The assessment of plant growth promoting (PGP) activities of Rhizobium showed differences between bacterial viability and bacterial PGP activity in terms of abiotic stress tolerance where bacterial PGP activity is interrupted before reaching the bacterial tolerance threshold. These results integrate a new concept of PGPR screening based on PGP activity under abiotic stress.

scholarly journals Isolation and Characterization of Staphylococcus aureus From Food of Bovine Origin in Mekelle, Tigray, Ethiopia

2020 ◽  
Vol 14 (1) ◽  
pp. 234-241
Author(s):  
Million Weldeselassie ◽  
Getachew Gugsa ◽  
Ashwani Kumar ◽  
Yisehak Tsegaye ◽  
Nesibu Awol ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Antibiotic Sensitivity ◽  
Raw Milk ◽  
Cross Sectional Study ◽  
Penicillin G ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
Isolation And Characterization ◽  
Sensitivity Pattern ◽  
Significant Difference

Background: Among Food-borne diseases, S. aureus is a leading cause of gastroenteritis resulting from the consumption of contaminated food. Objectives: The study aimed to isolate and characterize S. aureus from raw milk, yogurt and meat and determine its antibiotic sensitivity pattern. Materials and Methods: A cross-sectional study was conducted from December 2014 to June 2015 in Mekelle. A total of 284 samples were collected purposively. Enumeration of total viable bacteria count (TVBC), bacteriological isolation and identification, antimicrobial susceptibility testing, as well as PCR amplification of fem A and mec A genes were performed. Chi-square (χ2) and one way ANOVA tests were used for analysis. Results: Overall TVBC mean was found to be 1.29x108cfu/ml/g. The highest TVBC (1.38x108cfu/ml) was from the yogurt sample and the lowest (1.26x108cfu/g) was from meat. The overall prevalence of coagulase positive S. aureus (CoPS) was 39.1% (111) and of the smaples, 51(56.04%), 38(26.20%) and 22(45.83%) were isolated from meat, raw milk, and yogurt, respectively. There was a statistically significant difference (p<0.05) among the different sample types and sources in the prevalence of S. aureus. Almost half of the CoPS isolates were sensitive to Tetracycline, Gentamycin, and Kanamycin, but resistant to Amoxicillin (96.9%) and Penicillin G (93.8%). Moreover, 93.75% of the isolates developed multidrug resistance. All isolates carried the fem A gene and among these isolates, 12 (37.5%) carried mec A gene. Conclusion: The present study revealed that foods of bovine origin of the study area are found to be having less bacteriological quality, high prevalence of CoPS and development of drug resistance.

Service Quality Perception towards E-CRM Practices among Customers of Private and Public Sector Banks of Rajasthan Using SERVQUAL Method

2018 ◽  
Vol 7 (3) ◽  
pp. 22
Author(s):  
Neeti Kasliwal ◽  
Jagriti Singh
Keyword(s):  
Public Sector ◽  
Service Quality ◽  
Banking Sector ◽  
Sampling Technique ◽  
Vital Role ◽  
Public Sector Banks ◽  
Significant Difference ◽  
Private And Public ◽  
Score Method ◽  
Public Banks

Banking sector is growing rapidly and playing a vital role in the economic development of the nation. Both private and public sector banks are giving more priority to service quality to satisfy their customers. For this, banks are now emphasizing on E-CRM practices to carry out transactions and communicate with their customers. The purpose of this research is to assess the service quality among private and public banks in Rajasthan. Purposive sampling technique has been employed to collect the data from three private banks and three banks from public. To analyze the data, descriptive statistics, Mean score method and t test have been used. Results indicates that there is a significant difference in consumer’s perception of service quality dimensions related to E-CRM practices provided by selected private and public sector banks of Rajasthan..The findings of this research will help policy makers of banking sector to set customer oriented policies.

scholarly journals Antibiotic sensitivity and RAPD-PCR studies on cultivable gut bacteria from Indian Medicinal Leech—Hirudinaria granulosa

2020 ◽  
Vol 81 (1) ◽  
Author(s):  
Dhaval Patel ◽  
Khushbu Koriya ◽  
Pinkal Patel ◽  
Jyoti Solanki ◽  
Suresh Mesara ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Medicinal Leech ◽  
Gut Bacteria ◽  
Rapd Pcr

A Simultaneous Cluster Analysis of Cognitive, Emotional, and Personality Factors and Insomnia and Sleep Quality Among Earthquake Victims

2019 ◽  
Vol 13 (4) ◽  
pp. 745-752 ◽  
Author(s):  
Habibolah Khazaie ◽  
Ali Zakiei ◽  
Saeid Komasi
Keyword(s):  
Cluster Analysis ◽  
Emotion Regulation ◽  
Sleep Quality ◽  
Personality Traits ◽  
Experiential Avoidance ◽  
Dysfunctional Attitudes ◽  
Personality Factors ◽  
Cross Sectional ◽  
Mean Values ◽  
Significant Difference

ABSTRACTObjectiveThe current study compares the measures of sleep quality and intensity of insomnia based on the clustering analysis of variables including dysfunctional beliefs and attitudes about sleep, experiential avoidance, personality traits of neuroticism, and complications with emotion regulation among the individuals struck by an earthquake in Kermanshah Province.MethodsThis study is a cross-sectional study that was carried out among earthquake victims of Kermanshah Province (western Iran) in 2017. Data were gathered starting 10 days after the earthquake and lasted for 2 weeks; of 1,200 standard questionnaires distributed, 1,001 responses were received, and the analysis was performed using 999 participants. The data analysis was carried out using a cluster analysis (K-mean method).ResultsTwo clusters were identified, and there is a significant difference between these two clusters in regard to all of the variables. The cluster with higher mean values for the selected variables shows a higher intensity of insomnia and a lower sleep quality.ConclusionsConsidering the current results, it can be concluded that variables of dysfunctional attitudes and beliefs about sleep, experiential avoidance, the personality traits of neuroticism, and complications with emotion regulation are able to identify the clusters where there is a significant difference in regard to sleep quality and the intensity of insomnia. (Disaster Med Public Health Preparedness. 2019;13:745–752)

scholarly journals Diversity, Abundance, and Foraging Behavior of Ants (Hymenoptera: Formicidae) Scavenging on American Cockroach in Various Habitats of Nasarawa State, Nigeria

2020 ◽  
Vol 43 (4) ◽  
Author(s):  
Mohammed Ahmed Ashigar ◽  
Abdul Hafiz Ab Majid
Keyword(s):  
Rural Communities ◽  
Relative Abundance ◽  
Periplaneta Americana ◽  
Vital Role ◽  
American Cockroach ◽  
Conservation Implications ◽  
Residential Communities ◽  
Food Borne ◽  
Significant Difference ◽  
Paratrechina Longicornis

Ants play a vital role in removing dead arthropods from the environment. Complex foraging patterns are used by ants to locate food items and overwhelm even larger insects such as cockroach. Consequently, the biotic interaction between the ants and the American cockroach, Periplaneta americana, another home infesting and a vector of major food-borne diseases, may lead to microbial handover and ease the spread of mechanically transmitted human pathogenic microbes. This study was done to determine the diversity and abundance of cockroach-foraging ants in Nasarawa State, Nigeria. Ten households were randomly selected from 14 locations: 5 residential communities from three most urbanized areas (Lafia, Akwanga, and Keffi) and 5 rural communities. Four remaining locations were nonresidential from Lafia and Akwanga, respectively. A total of 1,364 ants belonging to three subfamilies (Myrmicinae, Formicinae, and Ponerinae) were collected from 140 households. Pheidole rugaticeps Emery recorded the highest relative abundance (52%) followed by Pheidole decarinata Santschi (16%), Pheidole sp. (17%), Camponotus maculatus (7%), Paratrechina longicornis (7%), while both Crematogaster sp. and Brachyponera sennaarensis recorded the lowest relative abundance (1%). There is a significant difference in the species diversity between the urban and the rural communities. Pheidole rugaticeps, P. decarinata, Pheidole sp., and P. longicornis were more organized in foraging and operating in group by recruiting nest-mate for collecting fragments of dead cockroach. Studies on the epidemiology, conservation implications, and biocontrol potentials of these Pheidole species are recommended.

scholarly journals Duckweed Species Genotyping and Interspecific Hybrid Discovery by Tubulin-Based Polymorphism Fingerprinting

2021 ◽  
Vol 12 ◽  
Author(s):  
Luca Braglia ◽  
Massimiliano Lauria ◽  
Klaus J. Appenroth ◽  
Manuela Bog ◽  
Diego Breviario ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Lemna Minor ◽  
Pcr Amplification ◽  
Food Supplement ◽  
Universal Primers ◽  
Special Focus ◽  
Length Polymorphisms ◽  
Accurate Comparison ◽  
New Perspective ◽  
Β Tubulin

Duckweeds (Lemnaceae) are the smallest and fastest-growing angiosperms. This feature, together with high starch production and good nutritional properties, makes them suitable for several applications, including wastewater treatment, bioenergy production, or feed and food supplement. Due to their reduced morphology and great similarity between diverse species, taxonomic identification of duckweeds is a challenging issue even for experts. Among molecular genotyping methods, DNA barcoding is the most useful tool for species identification without a need for cluster analysis. The combination of two plastid barcoding loci is now considered the gold standard for duckweed classification. However, not all species can be defined with confidence by these markers, and a fast identification method able to solve doubtful cases is missing. Here we show the potential of tubulin-based polymorphism (TBP), a molecular marker based on the intron length polymorphisms of β-tubulin loci, in the genomic profiling of the genera Spirodela, Landoltia, and Lemna. Ninety-four clones were analyzed, including at least two representatives of each species of the three genera, with a special focus on the very heterogeneous species Lemna minor. We showed that a single PCR amplification with universal primers, followed by agarose gel analysis, was able to provide distinctive fingerprinting profiles for 10 out of 15 species. Cluster analysis of capillary electrophoresis–TBP data provided good separation for the remaining species, although the relationship between L. minor and Lemna japonica was not fully resolved. However, an accurate comparison of TBP profiles provided evidence for the unexpected existence of intraspecific hybrids between Lemna turionifera and L. minor, as further confirmed by amplified fragment length polymorphism and sequence analysis of a specific β-tubulin locus. Such hybrids could possibly correspond to L. japonica, as originally suggested by E. Landolt. The discovery of interspecific hybrids opens a new perspective to understand the speciation mechanisms in the family of duckweeds.

scholarly journals The effect of public space indicators on the rural district’s life quality in Kuhdasht county, Iran

2021 ◽  
pp. 205-219
Author(s):  
Ahmad Roumiani ◽  
Taghi Ebrahimi Salari ◽  
Hamideh Mahmoodi ◽  
Mofid Shateri
Keyword(s):  
Public Space ◽  
Significant Relationship ◽  
Life Quality ◽  
Vital Role ◽  
The Body ◽  
Quality Enhancement ◽  
The Public ◽  
Quality Life ◽  
Significant Difference ◽  
Life Enhancement

This article evaluates nine rural districts in Kuhdasht county, Iran, with a population of 3535 between 2013–2016. We address the following two questions: First, what are the most important criteria and effective indicators in the rural population’s quality life enhancement? Second, is there any significant relationship between the public space indicators and quality life enhancement in the case study area? Six factors, including perceptual vision, buildings skeletons, culture and communities, activities, social interaction, and the environment from local peoples’ perspectives, explained 52.6 percent of the total variable variances. The Friedman test showed a significant difference among criteria of esthetics, semantic-perceptual, and activity-based functional at the alpha level of 0.01. The fitting growth regression model showed that the positive effect of the public space indicators on the rural population’s vitality and dynamism quality enhancement was about 0.723, indicating a significant relationship between them. It also stated a vital role of public space indicators in the rural population’s vitality and dynamism quality enhancement in the study area. The most important indicators were those of economic, social, and cultural dynamism and the body and space indicators.

scholarly journals The effect of cold-chain re-introduction on the molecular integrity of rocuronium bromide

2021 ◽  
Author(s):  
D Vermeulen ◽  
M Wooding ◽  
K Outhoff ◽  
T Dippenaar
Keyword(s):  
Degradation Products ◽  
Rapid Sequence Induction ◽  
Vital Role ◽  
Cold Chain ◽  
Clinical Effects ◽  
Rocuronium Bromide ◽  
Onset Of Action ◽  
Duration Of Action ◽  
Significant Difference ◽  
Temperature Exposure

Introduction: This study aimed to examine the effect of breakages and re-introduction into cold chain on the rocuronium bromide compound. Rocuronium bromide is frequently used in routine theatre lists and plays a vital role in modified rapid sequence induction and intubation for emergency patients who have contraindications to the primarily used muscle relaxant, succinylcholine. With the current practice of removing the drug from, and then reintroducing it into the cold chain, unpredictable clinical effects, including delayed onset of action and shortened duration of action have been observed. This may pose significant risks to the patient. Methods: Rocuronium bromide was subjected to different clinically applicable storage and temperature scenarios, after which the compound was analysed for integrity and quantities of the active compound, including detection of possible degradation products, by mass spectrometry, and compared to cold chain control samples. Results: There were no significant differences between any of the temperature exposure groups (18 °C or 24 °C) or between single or double exposures at these temperatures. No statistically significant difference could be demonstrated between the two control groups (cold chain preserved and room temperature controlled) with testing done at weeks one and six. However, week twelve analysis revealed a statistically significant result which translates to a 26 μg/ml difference, which clinically would have no effect. Substantial results were obtained with a secondary exposure to air; which lead to a 20% decrease in rocuronium concentration (p = 0.02). Conclusion: Practice should be adapted by keeping careful documentation as to when cold-chain was broken, and when the recommended 12 week period will lapse. Vial sharing as a standard is not recommended. If small quantities are repeatedly withdrawn from the vial during a prolonged case, the unused contents should be discarded after eight hours.

scholarly journals Texts with Various Levels of Hardness, Reading Comprehension and Reading Motivation: I+1 Versus I-1

2019 ◽  
Vol 8 (1) ◽  
pp. 60-77 ◽  
Author(s):  
Ehsan Namaziandost ◽  
Fariba Rahimi Esfahani ◽  
Mehdi Nasri
Keyword(s):  
Reading Comprehension ◽  
Reading Motivation ◽  
Vital Role ◽  
Current Level ◽  
Difficulty Level ◽  
Extensive Reading ◽  
Efl Learners ◽  
Significant Difference ◽  
Comprehensible Input ◽  
Post Test

Considering the vital role of comprehensible input, this study attempted to compare the effects of input with various difficulty levels on Iranian EFL learners’ reading comprehension and reading motivation. To fulfil this objective, 54 Iranian pre-intermediate EFL learners were selected from two intact classes (n = 27 each). The selected participants were randomly assigned to two equal groups, namely “i+1” (n=27) and “i-1” group (n=27). Then, the groups were pretested by a researcher-made reading comprehension test. After carrying out the pre-test, the treatment (i.e., extensive reading at different levels of difficulty) was practiced on the both groups. The participants in “i+1” group received reading passages beyond the current level, on the other hand, the “i-1” group received those reading passages which were below their current level. After the instruction ended, a modified version of pre-test was conducted as posttest to determine the impacts of the treatment on the students’ reading comprehension. The obtained results indicated that there was a significant difference between the post-tests of “i+1” and “i-1” groups. The findings showed that the “i+1” group significantly outperformed the “i-1” group (p < .05) on the post-test. Moreover, the findings indicated that “i+1” group’s motivation increased after the treatment. The implications of the study suggest that interactive type of input is beneficial to develop students’ language skills. Keywords: Comprehensible Input; Extensive reading; Foreign language reading motivation; Input; Reading comprehension; Text difficulty level

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