scholarly journals Antibacterial Activity of Environmentally Sustainable Polyurethane Based Composites from Castor Oil

2021 ◽  
Vol 37 (3) ◽  
pp. 755-758
Author(s):  
D. Jeba ◽  
K. R. Sheeja
Keyword(s):  
Streptococcus Mutans ◽  
Castor Oil ◽  
Bacterial Pathogens ◽  
Diffusion Method ◽  
Medical Applications ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Environmentally Sustainable ◽  
Potential Activity

In this present work, soft and hard antibacterial polyurethane sheets of different composition have been synthesized from castor oil based polyurethane and which was reinforced with natural and synthetic fibres such as jute, sisal, hemp and glass. The activity of polyurethane sheets and selected antibiotics was evaluated against four bacterial pathogens including Staphylococcusaureus, Streptococcus mutans, E.coli, Pseudomonas aeruginosausing modified disc diffusion method. Among the ten samples, hard polyurethanes displayed potential activity against bacterial pathogens E.coli and Streptococcus mutans. Polyurethane sheets showed the highest activity against E.coli which is comparable with zone of inhibition exhibited by streptomycin. Further studies are needed to improve the polyurethane sheets for medical applications.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

scholarly journals IDENTIFICATION OF ANTIBACTERIAL PROPERTY OF DIFFERENT MEDICINAL PLANT EXTRACTS

2020 ◽  
pp. 39-42
Author(s):  
SWETA KUMARI ◽  
VIRENDRA VAISHNAV
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
High Efficiency ◽  
Curcuma Longa ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Cinnamomum Tamala ◽  
Medicinal Plant Extracts

Objective: The objective of this study was to evaluate the antibacterial activity of some plant extracts they are-Curcuma longa, Cinnamomum tamala, Ocimum tenuiflorum, Azadirachta indica, Datura stramonium, Zingiber officinale, Punica granatum and Cinnamomum cassia against Staphylococcus aureus and E. coli. Methods: The evaluation of antibacterial activity for different extracts of each plant was carried out by using the disc diffusion method and by pouring technique to determine the minimum inhibitory concentration (MIC). Results: In the disc diffusion method the plant extracts showed zone of inhibition ranging 02.0 to 16.0 mm against bacteria and In pouring technique plant extracts showed positive control against pure culture of a bacterium and their efficiency in terms of MICs where ranged from 0.2 gm/ml to 0.8 gm/ml. The extracts of Curcuma longa and Cinnamomum tamala shown high efficiency then other plant extracts in terms of zone of inhibition and also in pouring technique less amount of extracts was inhibited the growth of bacteria. Conclusion: This study indicates that extracts of these plants have antibacterial activity against bacteria. This report supports their use in the treatment of infections caused by bacteria.

scholarly journals Antibacterial properties of Carum copticum essential oil against Streptococcus mutans and Streptococcus sobrinus isolated from canine dental plaque

2017 ◽  
Vol 62 (No. 12) ◽  
pp. 654-660 ◽  
Author(s):  
SN Gharajalar ◽  
M. Hassanzade
Keyword(s):  
Essential Oil ◽  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Minimum Bactericidal Concentration ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Streptococcus Sobrinus ◽  
Disc Diffusion Method ◽  
Carum Copticum ◽  
Resistance Patterns

Dental caries is amongst the most prevalent oral diseases in both humans and dogs. Streptococcus mutans and Streptococcus sobrinus (mutans streptococci) are the major cariogenic bacteria isolated from dental caries. Since these bacteria generally show resistance to common antibiotics, natural products such as plant essential oils could be a good substitute. For this study, we aimed to evaluate the antibacterial activity of Carum copticum essential oil against Streptococcus mutans and Streptococcus sobrinus. Twenty canine dental plaque samples were collected and the presence of S. mutans and S. sobrinus in the samples was confirmed using biochemical, culture and polymerase chain reaction (PCR) assays. The resistance patterns of isolates were determined using a disc diffusion method according to the Clinical Laboratory Standards Institute protocol against the following antimicrobials: chloramphenicol, tetracycline, penicillin, erythromycin, ceftriaxone, cefotaxime, vancomycin and azithromycin. The antibacterial activities of Carum copticum essential oil were based on the disc diffusion method as well on a determination of the minimum inhibitory (MIC<sub>50</sub>) and minimum bactericidal concentration values. S. mutans and S. sobrinus were isolated in 8 (40%) and 2 (10%), respectively, of plaque samples. Most of these isolates were determined to display multidrug resistance patterns to the eight antibiotics evaluated. Screening of the antibacterial activity of the essential oil indicated that MIC<sub>50</sub> and minimum bactericidal concentration values were 20 µg/ml and 80 µg/ml, respectively, and that the zone of inhibition in the disc diffusion method ranged from 2 to 5 mm for serial concentrations of the essential oil. Based on our results, we suggest that Carum copticum essential oil exerts antibacterial effects against Streptococcus mutans and Streptococcus sobrinus and may be a useful treatment for carious lesions with bacterial aetiologies.

scholarly journals Antibacterial Activity of Eight Medicinal Plants against Multidrug Resistant Escherichia coli and Salmonella spp. isolated from Broiler Meat

2020 ◽  
Vol 3 (4) ◽  
pp. 28-48
Author(s):  
Md. Rayhan Ali ◽  
Md. Omar Faruque ◽  
Md. Tarek Molla ◽  
Roksana Khanam ◽  
Shahin Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Standard Disc

The excess use of antimicrobial agents in the poultry industry is a significant reason for the gradual spread and increasing level of multidrug resistance bacteria. This article is based on a study in which the antibacterial activity of aqueous, methanolic, ethanolic and acetonic extracts of eight medicinal plants were evaluated by standard disc diffusion method against multidrug resistant Escherichia coli and Salmonella spp. isolated from broiler meat. The multidrug resistance was checked by commercially available antibiotics using standard disc diffusion method. The results have indicated that the ethanolic extract of Boerhaavia diffusa showed maximum zone of inhibition against Escherichia coli, while Asparagus racemosus showed maximum zone of inhibition against Salmonella spp. Other experimental plant extracts had showed moderate activity against these multidrug-resistant bacteria, which can also be considered as potential source of active beneficial phytochemicals. Proper management and application of these plant extracts may be a wonderful alternative of commercially available antibiotic to minimize the risk.

scholarly journals Хар мод /larix sibirica ldb./-ны мөчрийн боомын нян /bacillus anthracis/-гийн эсрэг идэвхит нэгдлийн судалгаа

2017 ◽  
pp. 58-66
Author(s):  
Эрдэнэбаатар П ◽  
Батхүү Ж ◽  
Ганболд Д ◽  
Оюунцэцэг Ш ◽  
Отгонбаатар Д ◽  
...  
Keyword(s):  
Methanol Extract ◽  
13C Nmr Spectroscopy ◽  
Larix Sibirica ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Inhibition Activity ◽  
Disc Diffusion Method ◽  
Isopimaric Acid ◽  
Causal Pathogen ◽  
Potential Activity

No Mongolian AbstractThe methanol extract and its water nonsoluble fraction of the stem of Larix sibirica Ldb. exhibited a good inhibition activity against M.luteus strain and anthrax causal pathogen B.anthracis 90, 120, 147, 178 by the disc diffusion method. Activity-guided isolation of the water nonsoluble resin fraction led to the isolation of isopimaric acid which molecular structure was determined by 1H, 13C NMR spectroscopy and MS methods. Isopimaric acid exhibited a potential activity against M.luteus bacterial strain (9.5 mm) and B.anthracis 90 –17 mm, 120–15 mm, 147–15 mm, 178–17 mm, respectively.

scholarly journals Synthesis, Characterization and Study the Biological Activity of New Morpholine Derivative

2015 ◽  
Vol 12 (4) ◽  
pp. 761-773 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Schiff Base ◽  
Aromatic Aldehydes ◽  
Diffusion Method ◽  
Chloroacetyl Chloride ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Chemical Structures ◽  
Triethyl Amine ◽  
Morpholine Derivative

A new series of morpholine derivative were prepared by reacting the morpholine with ethyl chloro acetate in the presence triethylamine as an catalyst and benzene as a solvent gave the ethyl morpholin-4-ylacetate reaction with hydrazine hydrate and ethanol as a solvent gave the 2-(morpholin-4-yl)acetohydrazide gave series of Schiff base were prepared by reacting 2-(morpholin-4- yl)acetohydrazide with different aromatic aldehydes and ketons . The new series of (3-9 )were synthesis by reaction of Schiff base (10-14) with chloroacetyl chloride, triethyl amine as an catalyst and 1,4dioxane as a solvent .The chemical structures of the synthesis compound were identified by spectral methods their [ IR ,1H-NMR and 13C-NMR ].The synthesised compounds were screened for antibacterial activity and antifungal activity promising by disc diffusion method by measuring the zone of inhibition and the results were compared to standard drugs ciprofloxacin .

scholarly journals Antimicrobial Activity of Cymbopogon (Lemongrass) on Enterococci

2021 ◽  
pp. 684-690
Author(s):  
A. Sai Sanjith ◽  
N. P. Muralidharan
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Dilution Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Antibiotic Susceptibility Test ◽  
Lemongrass Oil ◽  
Test Organisms ◽  
Broth Dilution

Aim: The aim of this study was to investigate antimicrobial activity against Enterococcus bacteria. Materials and Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the Broth Dilution Method. The antibiotic susceptibility test against the test organisms was performed by the Disc Diffusion Method. Results and Discussion: Antimicrobial activity of Cymbopogon oil was examined against common Enterococcus bacteria using broth microdilution assay and the disc diffusion method. Zone of inhibition was seen when Cymbopogon oil was kept with enterococcus bacteria, this proves to be bacteriostatic. Conclusion: Lemongrass oil proved to be bacteriostatic against Enterococcus, further research to be done to implement Lemongrass oil as an antibacterial agent.

scholarly journals A Comparative Study of Analgesic, Antidiarrhoeal and Antimicrobial Activities of Methanol and Acetone Extracts of Fruits Peels of Limonia acidissima L. (Rutaceae)

2020 ◽  
Vol 10 (1-s) ◽  
pp. 62-65
Author(s):  
Fahadul Islam ◽  
A.K. Azad ◽  
Md. Faysal ◽  
Md. A.K. Azad ◽  
Saiful Islam ◽  
...  
Keyword(s):  
Diclofenac Sodium ◽  
Antimicrobial Activities ◽  
Significant Inhibition ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Standard Drug ◽  
Limonia Acidissima ◽  
Acetone Extracts

Objectives: Present study was designed to evaluate Analgesic, Antidiarrhoeal and Antimicrobial activities of methanol and acetone extracts of fruit peels of Limonia acidissima L. by different methods. Method: The analgesic activity of the samples was studied using acetic acid- induced writhing model in mice. Castor oil-induced antidiarrheal activity was observed by Thomas method and antimicrobial activity was monitored by disc diffusion method. Results: Limonia acidissima L. inhibited 60.53 % and 59.65 % writhing of methanol as well as acetone fruit peels extracts, respectively, compared to standard drug Diclofenac Na inhibited 78.07 % writhing. At higher dose (500 mg/kg) of the methanol and acetone fruit peels extracts, significant inhibition 47.13 and 44.83 % of characteristic diarrhoeal feces was observed, respectively, as well as at lower dose (250 mg/kg) of the both extracts, inhibition 34.45 and35.63 %. Mention able on average 12mm zone of inhibition was observed of both extract at 250µg/disc and 500µg/disc compare to zone of inhibition 36mm of ciprofloxacin at 50µg/disc. Conclusion: From the above results, it will be very much possible source for an isolating lead compound for curing the numerous disorders. Keywords: Limonia acidissima L. Fruit peels, Diclofenac sodium, Loperamide, Ciprofloxacin.

scholarly journals Gayatri Mantra Chanting Helps Generate Higher Antimicrobial Activity of Yagya’s Smoke

2018 ◽  
Vol 1 (1) ◽  
pp. 09-14 ◽  
Author(s):  
Ruchi Singh ◽  
Sunil Kumar Singh
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Inhalation Therapy ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Plant Materials

Yagya (known as a fire-ritual) is an ancient Vedic Indian method of herbal inhalation therapy. It is also described as an excellent mode of environmental purification. Here, a comparative study of antimicrobial activity of smoke extract of Yagya and non-Yagya has been performed. ‘Yagya’ in this text, refers to the burning of wood, specific types of dried medicinal plant materials (hawan samagri) along with ghee with the citation of mantras, whereas the term Non-Yagya implies burning of wood, hawan samagri, along with ghee. In non-Yagya the mantra citation has not been done. An apparatus was designed to simulate the burning process, and the smoke fraction was captured for antimicrobial activity on human pathogens i.e Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, and Salmonella typh. Antimicrobial activity was performed using disc diffusion method. Antimicrobial data revealed that the ‘smoke-extract’ obtained from Yagya experiments with mantra chanting had higher zone of inhibition values compared to that of same experiment conditions except presence of mantra chanting suggesting an important role of the mantra chanting in Yagya for medicinal applications

scholarly journals ANTIMICROBIAL EFFICACY OF CARABAO GRASS LEAVES ON STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 7 (1) ◽  
pp. 384-397
Author(s):  
Dani Aiko Perral Garduque ◽  
Kyrie Radiance Ganal Mateo ◽  
Shalom Moyinoluwa Adejumoke Oyinloye ◽  
Jo Anne kristine Lalas Lucero
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
The Philippines ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Treatment And Prevention ◽  
Testing Center

Antimicrobial Efficacy of Carabao Grass (Paspalum conjugatum) leaves on Staphylococcus aureus Dani Aiko Garduque1, Kyrie Radiance Mateo1, Shalom Moyinoluwa Oyinloye1, and Jo Anne Kristine Lalas-Lucero2   1Student, Adventist University of the Philippines 2Faculty, Adventist University of the Philippines   [email protected]   Abstract   Introduction: In the Philippines, the Paspalum conjugatum or known by its common name as “carabao grass” is usually consumed for livestock as well as for landscaping. Paspalum conjugatum has been known to have phytochemicals that kill microorganisms. This experimental research determined the antimicrobial efficacy of Paspalum conjugatum against Staphylococcus aureus.   Methods: Samples of the carabao grass was purchased, washed and then dried. An extract was obtained from the sample. The extract was then sent to an accredited testing center for phytochemical test to identify the different components of the plant extract. The antimicrobial activity of the paspalum conjugatum extract was then determined through the use of the disc diffusion method. The zone of inhibition of Paspalum Conjugatum was also compared to a control standard such as oxacillin.   Results: The results of the study revealed to have abundant constituents of sterols, flavonoids and tannins; moderate levels of triterpenes, saponins and glycosides; and traces of alkaloids. The antimicrobial testing showed results that Paspalum Conjugatum had 10mm zone of inhibition which means that it has a complete inhibitory activity with severe reactivity against the tested organism, staphylococcus aureus.   Discussion: The study concludes that Paspalum conjugatum can be used to eliminate staphylococcus aureus. It is recommended that further studies be conducted for the use of Paspalum conjugatum in the treatment and prevention of diseases caused by staphylococcus aureus. Keywords:  paspalum conjugatum, carabao grass, staphylococcus aureus  

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