scholarly journals Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks

Author(s):  
Desrina Desrina ◽  
Slamet Budi Prayitno ◽  
Alfabetian Harjuno Condro Haditomo ◽  
Rusthesa Latritiani ◽  
Sarjito Sarjito

Abstract. Desrina, Prayitno S B, Haditomo A H C, Latritiani R, Sarjito S. 2020. Detection of Enterocytozoon hepatopenaei (EHP) DNA in the polychaetes from shrimp ponds suffering white feces syndrome outbreaks. Biodiversitas 21: 369-374. Enterocytozoon hepatopenaei (EHP) is the newly emerging pathogen of farmed shrimp in Asia. EHP was associated with white feces syndrome (WFS) in shrimp because the spores existed in the feces of shrimp with WFS. Polychaetes are benthic invertebrates and potential to contribute to EHP outbreaks in a pond by acquiring the pathogen and transfer it to shrimp upon feeding. The objective of this study was to determine (1) the occurrence of EHP in polychaetes with Giemsa staining of the coelomic fluid, 1-step PCR and histopathology and (2) to determine pathological changes of EHP infected tissues. Polychaetes were obtained from 20 shrimp ponds located in 9 districts along the North Coast of Java Island, Indonesia. There were no EHP spores detected in the stained coelomic fluid. The 1-step PCR analysis was able to detect the EHP DNA (prevalence was 10%). The Alignment of EHP DNA sequence found in polychaetes showed 100% identity with a published sequence. Histopathological examination showed hypertrophy cells and nuclei, however, no EHP mature spores were found in the epithelial cells on the mucosal layer of the stomach and intestines. Although methods used did not give the convergence results, however, a result of PCR and sequence analysis showed that EHP occurred in the polychaetes in the WFS infected ponds at low point prevalence.

2016 ◽  
Vol 47 (2) ◽  
pp. 194
Author(s):  
Widiatmaka Widiatmaka ◽  
Wiwin Ambarwulan ◽  
Yudi Setiawan ◽  
Muhamad Yanuar Jarwadi Purwanto ◽  
Taryono Taryono ◽  
...  

Shrimp is a commodity that is increasingly in demand. The limited land resources implies the need of effective land use planning. The objective of this study was to assess land suitability for brackish water shrimp ponds, which then will be recommended for pond development in the north coast of Tuban, Indonesia. Analytical hierarchy process (AHP) were used to obtain the weight of the different criteria consisted of soil characteristics, topographic, water quality, and infrastructure criteria. The suitable land for brackish water shrimp ponds was determined by weighted overlay in GIS. The results show that the study area contains highly suitable land for brackish water shrimp ponds. Land use and land cover map was interpreted from 2014 SPOT 5 imagery. The area recommended for brackish water shrimp pond wasdelineated by taking into account the suitability and the constraints of land use and land cover.


EMPIRISMA ◽  
2008 ◽  
Vol 27 (2) ◽  
Author(s):  
Fathimatuz Zahra Dan Abdul Azis

Pati is a region on the north coast, according to the hypothesis of the researcher, the region is divided into three categories. The northern regions are more religious, the central is more plural, while the southern region is in the middle. In the central region there are many relics of tombs believed to be the those of the Muslim proselytizers in the area of Pati. The one that attracts the researcher is a tomb in the Gambiran area, where there are five local Muslim saints buried, one of them belons to mbah Hendro Kusumo, the son of Syech Ahmad Mutamakkin. This article attempts to trace back the spreading of Islam in Pati based on the existence of thetomb of Mbah Hendro Kusumo. It wants to answer question of whethere the existence of his tomb is due to his studying there or marital relationship, and how it relates to the spreading of Islam.Keywords: Mbah Hendro Kusumo, Traces of Islamic Dakwah, Islam


1968 ◽  
Vol 22 ◽  
pp. 44-79 ◽  
Author(s):  
William E. Taylor

The Tyara site, KkFb-7 in the National Museum catalogue and site file, faces the north coast of the Ungava mainland and rests on the west shore of Sugluk Island (Fig. 1). That island stands about five hundred yards from the mainland and from Sugluk Inlet, one of the few good harbors on that coast. This handsome little island, about one and one-half miles long and as wide, consists of rounded, rugged, hardrock hills that shelter well-vegetated, generally flat-floored valleys. The valleys often contain marshy patches. The shore, of variable incline, is quite jagged, a result of abrupt rock outcrops projecting seaward from brief stretches of sandy beach. The shore facing the mainland is, therefore, quite convenient for small boat use. Dark grey gneisses seem to predominate, although they are often cut by dykes and veins of lighter material, notably quartz. The dense, green valley and hillside vegetation includes willows, mosses, grasses, lichens, and a pleasant profusion of arctic wild flowers (Polunin 1948, Pt. III). I was told at Sugluk that at the head of the inlet, willows, growing in protected situations, reach the thickness of a man's wrist.


1996 ◽  
Vol 36 (3) ◽  
pp. 299 ◽  
Author(s):  
TS Andrews ◽  
RDB Whalley ◽  
CE Jones

Inputs and losses from Giant Parramatta grass [GPG, Sporobolus indicus (L.) R. Br. var. major (Buse) Baaijens] soil seed banks were quantified on the North Coast of New South Wales. Monthly potential seed production and actual seed fall was estimated at Valla during 1991-92. Total potential production was >668 000 seeds/m2 for the season, while seed fall was >146000 seeds/m2. Seed fall >10000 seeds/m2.month was recorded from January until May, with further seed falls recorded in June and July. The impact of seed production on seed banks was assessed by estimating seed banks in the seed production quadrats before and after seed fall. Seed banks in 4 of the 6 sites decreased in year 2, although seed numbers at 1 damp site increased markedly. Defoliation from mid-December until February, April or June prevented seed production, reducing seed banks by 34% over 7 months. Seed banks in undefoliated plots increased by 3300 seeds/m2, although seed fall was estimated at >114 000 seeds/m2. Emergence of GPG seedlings from artificially established and naturally occurring, persistent seed banks was recorded for 3 years from bare and vegetated treatment plots. Sown seeds showed high levels of innate dormancy and only 4% of seeds emerged when sown immediately after collection. Longer storage of seeds after collection resulted in more seedlings emerging. Estimates of persistent seed banks ranged from 1650 to about 21260 seeds/m2. Most seedlings emerged in spring or autumn and this was correlated with rainfall but not with ambient temperatures. Rates of seed bank decline in both bare and vegetated treatment plots was estimated by fitting exponential decay curves to seed bank estimates. Assuming no further seed inputs, it was estimated that it would take about 3 and 5 years, respectively, for seed banks to decline to 150 seeds/m2 in bare and vegetated treatments.


2021 ◽  
Vol 13 (5) ◽  
pp. 873
Author(s):  
Dimitra Konsta ◽  
Alexandra Tsekeri ◽  
Stavros Solomos ◽  
Nikolaos Siomos ◽  
Anna Gialitaki ◽  
...  

We use the Generalized Retrieval of Aerosol Surface Properties algorithm (GRASP) to compare with dust concentration profiles derived from the NMME-DREAM model for a specific dust episode. The GRASP algorithm provides the possibility of deriving columnar and vertically-resolved aerosol properties from a combination of lidar and sun-photometer observations. Herein, we apply GRASP for analysis of a Saharan dust outburst observed during the “PREparatory: does dust TriboElectrification affect our ClimaTe” campaign (PreTECT) that took place at the North coast of Crete, at the Finokalia ACTRIS station. GRASP provides column-averaged and vertically resolved microphysical and optical properties of the particles. The retrieved dust concentration profiles are compared with modeled concentration profiles derived from the NMME-DREAM dust model. To strengthen the results, we use dust concentration profiles from the POlarization-LIdar PHOtometer Networking method (POLIPHON). A strong underestimation of the maximum dust concentration is observed from the NMME-DREAM model. The reported differences between the retrievals and the model indicate a high potential of the GRASP algorithm for future studies of dust model evaluation.


Author(s):  
Xiaoyi Shen ◽  
Chang-Qing Ke ◽  
Bin Cheng ◽  
Wentao Xia ◽  
Mengmeng Li ◽  
...  

AbstractIn August 2018, a remarkable polynya was observed off the north coast of Greenland, a perennial ice zone where thick sea ice cover persists. In order to investigate the formation process of this polynya, satellite observations, a coupled ice-ocean model, ocean profiling data, and atmosphere reanalysis data were applied. We found that the thinnest sea ice cover in August since 1978 (mean value of 1.1 m, compared to the average value of 2.8 m during 1978–2017) and the modest southerly wind caused by a positive North Atlantic Oscillation (mean value of 0.82, compared to the climatological value of −0.02) were responsible for the formation and maintenance of this polynya. The opening mechanism of this polynya differs from the one formed in February 2018 in the same area caused by persistent anomalously high wind. Sea ice drift patterns have become more responsive to the atmospheric forcing due to thinning of sea ice cover in this region.


Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1366
Author(s):  
Cristina Soares ◽  
Sara Sousa ◽  
Susana Machado ◽  
Elsa Vieira ◽  
Ana P. Carvalho ◽  
...  

The total lipid content and lipidic profile of seaweeds harvested in the North Coast and purchased in Portugal were determined in this paper. The amount of total lipids in the different species of seaweeds varied between 0.7 ± 0.1% (Chondrus crispus) and 3.8 ± 0.6% (Ulva spp.). Regarding the fatty acid content, polyunsaturated fatty acids (PUFA) ranged between 0–35%, with Ulva spp. presenting the highest amount; monounsaturated fatty acids (MUFA) varied between 19 and 67%; and saturated fatty acids (SFA) were predominant in C. crispus (45–78%) and Gracilaria spp. (36–79%). Concerning the nutritional indices, the atherogenicity index (AI) was between 0.4–3.2, the thrombogenicity index (TI) ranged from 0.04 to 1.95, except for Gracilaria spp., which had a TI of 7.6, and the hypocholesterolemic/hypercholesterolemic ratio (HH) values ranged between 0.88–4.21, except for Gracilaria spp., which exhibited values between 0.22–9.26. The n6/n3 ratio was below 1 for most of the species evaluated, except for Ascophyllum nodosum, which presented a higher value, although below 2. Considering the PUFA/SFA ratio, seaweeds presented values between 0.11–1.02. The polycyclic aromatic hydrocarbons (PAHs) and aliphatic hydrocarbons (AHCs) contamination of seaweeds under study was also quantified, the values found being much lower than the maximum levels recommended for foodstuff.


Sign in / Sign up

Export Citation Format

Share Document