scholarly journals Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
Linda Suhartati ◽  
ISYANA KHAERUNNISA ◽  
ASEP GUNAWAN ◽  
RUKMIASIH RUKMIASIH ◽  
SRI DARWATI ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Broiler Chicken ◽  
Muscle Development ◽  
Short Communication ◽  
Negative Regulator ◽  
Silent Mutation ◽  
Muscle Weight ◽  
Myostatin Gene ◽  
Exon 1 ◽  
Meat Type

Abstract. Suhartati L, Khaerunnisa L, Gunawan A, Rukmiasih, Darwati S, Sumantri C, Rizqan. 2020. Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken. Biodiversitas 21: 3893-3897.  Myostatin is widely known as a negative regulator of skeletal muscle development by controlling hypertrophy and hyperplasia. Silent mutation in exon 1 (c.234G>A) was then thought to be associated with chicken growth performances. This study was designed to analyze polymorphisms of c.234G>A mutation of the myostatin gene and its effect on slaughtered weight in Indonesian Kampung and meat-type chicken. The SatI restriction enzyme was used for genotype determination through the PCR-RFLP technique. The effect of genotype on the slaughtered weight was analyzed using the General Linear Model (GLM) procedure. Genotyping was performed on 138 chicken from Kampung (n=67) and meat type (cobb Broiler) (n=71) chicken. The amplification product contained 226 bp nucleotides. This myostatin gene was polymorphic in both chicken population, produced 2 alleles (G and A), and 3 genotypes (GG, GA, and AA). The A allele had a higher frequency than the G allele in all populations. In this study, genotype of AA and GA had higher live weight, carcass weight, breast weight, thigh weight, drumsticks weight, wings weight, breast muscle weight, thighs muscle weight, drumsticks muscle weight than GG genotype of 12 weeks-old Kampung chicken. The results suggested that the c.234G>A mutation in exon 1 could be potentially recommended as a genetic marker for chicken slaughtered weight traits.

scholarly journals Genetic variation in the first intron and exon of the myostatin gene in several Indonesian cattle populations

2021 ◽  
pp. 1197-1201
Author(s):  
Peni Wahyu Prihandini ◽  
Almira Primasari ◽  
Aryogi Aryogi ◽  
Jauhari Efendy ◽  
Muchamad Luthfi ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Transforming Growth Factor ◽  
Association Studies ◽  
Negative Regulator ◽  
Reaction Products ◽  
Myostatin Gene ◽  
Mstn Gene ◽  
Synonymous Mutations ◽  
Intron 1 ◽  
Exon 1

Background and Aim: Myostatin (MSTN), a member of the transforming growth factor-β family, is a negative regulator of muscle mass. This study aimed to detect the genetic variation of the 1160 bp fragment of exon 1 and part of intron 1 of the MSTN gene in several cattle populations raised in Indonesia. Materials and Methods: Polymerase chain reaction products of the MSTN gene amplified from 92 animals representing 10 cattle populations (Peranakan Ongole [PO], Belgian Blue x PO cross, Rambon, PO x Bali cross, Jabres, Galekan, Sragen, Donggala, Madura, and Bali) were sequenced, compared, and aligned with bovine MSTN of Bos taurus (GenBank Acc. No. AF320998.1) and Bos indicus (GenBank Acc. No. AY794986.1). Results: Four nucleotide substitutions (nt 1045 and 1066 in intron 1; nt 262 and 418 in exon 1) and two indels (nt 807 and 869 in intron 1) were synonymous mutations. Among these substitutions, only the nt 262G>C and nt 418A>G loci were polymorphic in all populations, except Bali cattle. The frequencies of the nt 262C (0.82) and nt 418A (0.65) alleles were highest. For the nt 262G>C locus, the CC genotype had the highest frequency (0.66) followed by GC (0.30) and CC (0.03). For the nt 418A>G locus, the AG genotype had the highest frequency (0.52) followed by AA (0.39) and GG (0.09). Conclusion: The results, showing genetic variations in exon 1 and intron 1 of the MSTN gene, might be helpful for future association studies.

Genetic Diversity Identification and Haplotype Distribution of Myostatin Gene in Goats

2020 ◽  
Author(s):  
Cheng-Li Liu ◽  
Ri-Su Na ◽  
Wei-Wei Ni ◽  
Guang-Xin E ◽  
Yan-Guo Han ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Muscle Development ◽  
Haplotype Frequency ◽  
Negative Regulator ◽  
Myostatin Gene ◽  
Polymorphic Loci ◽  
Boer Goat ◽  
Haplotype Distribution ◽  
Meat Goats ◽  
Significant Difference

Background: Myostatin (MSTN) is a highly conserved protein that acts as a negative regulator of skeletal muscle growth. MSTN gene is closely associated with multiple biological functions and its mutations are directly linked to muscle development in different species. Loss of MSTN functionality causes the phenotype to appear in the form of ‘double musculature’, among others in cattle, sheep and house mice.Methods: The mixed DNA pool of Boer and Dazu black goats to sequence MSTN coding and noncoding regions. Snapshot typing technology was used to analyse three goat production types, namely, Boer (meat goats), Nubian (breast and meat dual-use goat), Dazu black (local breeds) and Youzhou black goats (local breeds). Polymorphic loci in MSTN were used from four goat populations to construct haplotypes and calculate haplotype frequency distribution through NETWORK and MEGA to construct a phylogenetic tree and visualize phylogenetic relationship.Result: From these populations, 18 haplotypes were constructed using 20 polymorphic loci. Phylogenetic analysis revealed a significant difference in the haplotype distribution of MSTN in different goat production types. The 18 haplotypes were divided into two clusters. The haplotypes carried by the Boer goat belonged to Cluster I and those carried by the Nubian goat and two local goat breeds belonged to both clusters. Chinese local goats and complex production goats carried more haplotypes of MSTN and had a richer genetic diversity than other production types did. Moreover, local and complex production goats had high-frequency haplotypes of the MSTN of meat goats and had high potential for breeding.

Myostatin silencing effect on basic helix–loop–helix transcription factors in caprine foetal fibroblast cells

2017 ◽  
Author(s):  
Biswajyoti Borah ◽  
Ajit Pratap Singh ◽  
Hamen Gogoi ◽  
Amlan Jyoti Phukan ◽  
Bikash Chandra Sarkhel
Keyword(s):  
Transcription Factors ◽  
Muscle Development ◽  
Pearson Correlation ◽  
Negative Regulator ◽  
Fibroblast Cells ◽  
Animal Cells ◽  
Myostatin Gene ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

Transgenic food animal production is one of the potential and need oriented research to mitigate the food crises of the world. In vitro gene silenced animal cells and making use of these cells for transgenesis one of the suitable way to produce productive animals. Myostatin is a negative regulator of muscle growth, has the potential to increase the muscle mass upon its silencing. Four Hush 29-mer anti- myostatin (MSTN) shRNA constructs were checked for myostatin gene silencing in caprine foetal fibroblast cells and its subsequent effect on basic helix– loop–helix (bHLH) transcription factors. These factors are necessary for the terminal differentiation, proliferation, and homeostasis of muscle development. Different shRNA constructs displayed 55.1 to 91.5% (p less than 0.01) of myostatin silencing in caprine foetal fibroblast cells and upregulation of myogenic gene. Upregulation of 7.97 to 111.67 % for MyoD, 77.0 % to 319.47 % for myogenin, 16.67 % to 138.0 % for Myf5 were observed . The Pearson correlation established a negative correlation between myostatin and genes under study. Result suggests that knockdown of MSTN a potential approach to improve caprine musculatures.

Myostatin: a modulator of skeletal-muscle stem cells

2005 ◽  
Vol 33 (6) ◽  
pp. 1513-1517 ◽  
Author(s):  
F.S. Walsh ◽  
A.J. Celeste
Keyword(s):  
Skeletal Muscle ◽  
Muscular Dystrophy ◽  
Muscle Development ◽  
Transforming Growth Factor ◽  
Neutralizing Antibody ◽  
Transforming Growth Factor Β ◽  
Negative Regulator ◽  
Mdx Mouse ◽  
Specific Expression ◽  
Myostatin Gene

Myostatin, or GDF-8 (growth and differentiation factor-8), was first identified through sequence identity with members of the BMP (bone morphogenetic protein)/TGF-β (transforming growth factor-β) superfamily. The skeletal-muscle-specific expression pattern of myostatin suggested a role in muscle development. Mice with a targeted deletion of the myostatin gene exhibit a hypermuscular phenotype. In addition, inactivating mutations in the myostatin gene have been identified in ‘double muscled’ cattle breeds, such as the Belgian Blue and Piedmontese, as well as in a hypermuscular child. These findings define myostatin as a negative regulator of skeletal-muscle development. Myostatin binds with high affinity to the receptor serine threonine kinase ActRIIB (activin type IIB receptor), which initiates signalling through a smad2/3-dependent pathway. In an effort to validate myostatin as a therapeutic target in a post-embryonic setting, a neutralizing antibody was developed by screening for inhibition of myostatin binding to ActRIIB. Administration of this antimyostatin antibody to adult mice resulted in a significant increase in both muscle mass and functional strength. Importantly, similar results were obtained in a murine model of muscular dystrophy, the mdx mouse. Unlike the myostatin-deficient animals, which exhibit both muscle hypertrophy and hyperplasia, the antibody-treated mice demonstrate increased musculature through a hypertrophic mechanism. These results validate myostatin inhibition as a therapeutic approach to muscle wasting diseases such as muscular dystrophy, sarcopenic frailty of the elderly and amylotrophic lateral sclerosis.

scholarly journals Sequencing the exon 1 of MSTN in Mongolian horse (Equus caballus)

2019 ◽  
Vol 27 (02) ◽  
pp. 3-7
Author(s):  
Sergelen B ◽  
Khaliunaa T ◽  
Myagmarsuren P
Keyword(s):  
Muscle Development ◽  
Equus Caballus ◽  
Reference Sequence ◽  
Amino Acid Difference ◽  
Muscular Tissue ◽  
Long Distance ◽  
Myostatin Gene ◽  
Mstn Gene ◽  
Exon 1 ◽  
Mongolian Horse

Myostatin (MSTN) is a protein responsible for muscular tissue differentiation, development, and growth in mammals. Studies on cattle, mice, pig, and dog have provided sample demonstrations of mutations on myostatin, which strongly affected animal Ankole93200phenotype, particularly muscle development. In horse, myostatin gene is usually studied with respect to racing performance. Most Mongolian native horses do race for long distance and such racing is a very popular sport in Mongolia. On the other hand, the MSTN gene also affects meat yield and quality. We partially sequenced of MSTN gene to explore its polymorphic features in Mongolian horse. A 238 bp long segment of exon 1 was sequenced, using 3130 Genetic Analyser and MEGAX. The sequence was registered with accession number LC216412 in the Gene bank database of NCBI and aligned to four reference sequences (GQ183900.1, KC708233.1, AY840554.2, NM 001081817) from this database. The nucleotide diversity was 0.0084, Tajima test statistic was -1.12. so that there were 233 invariable (monomorphic) sites and 5 variable (polymorphic) sites, in the group of animals compared. The Mongolian horse differed from the reference sequence at 3 sites. There was an amino acid difference in the partial sequence, in which isoleucine in the reference sequence was converted into valine. The results of the study indicate that the Mongolian horse seems to differ substantially from the reference genome of equus caballus. Sequencing of the full length of exon 1 or even the whole MSTN gene, including introns, is strongly advised, to get more insights with regard to the identification and characterization of variants at the myostatin locus in Mongolian horses.

scholarly journals A novel mechanism of myostatin regulation by its alternative splicing variant during myogenesis in avian species

2015 ◽  
Vol 309 (10) ◽  
pp. C650-C659 ◽  
Author(s):  
Sangsu Shin ◽  
Yan Song ◽  
Jinsoo Ahn ◽  
Eunsoo Kim ◽  
Paula Chen ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Muscle Development ◽  
Muscle Growth ◽  
Avian Species ◽  
Negative Regulator ◽  
Cell Proliferation And Differentiation ◽  
Exon 1 ◽  
Splicing Isoforms

Myostatin ( MSTN) is a key negative regulator of muscle growth and development, and an increase of muscle mass is achieved by inhibiting MSTN signaling. In the current study, five alternative splicing isoforms of MSTN mRNAs in avian species were identified in various tissues. Among these five, three truncated forms of myostatin, MSTN-B, -C, and -E created premature stop codons and produced partial MSTN prodomains encoded from exon 1. MSTN-B is the second dominant isoform following full-length MSTN-A, and their expression was dynamically regulated during muscle development of chicken, turkey, and quail in vivo and in vitro. To clarify the function of MSTN-B, two stable cell lines of quail myoblasts (QM7) were generated to overexpress MSTN-A or MSTN-B. Interestingly, MSTN-B promoted both cell proliferation and differentiation similar to the function of the MSTN prodomain to counteract the negative role of MSTN on myogenesis. The coimmunoprecipitation assay revealed that MSTN-B binds to MSTN-A and reduces the generation of mature MSTN. Furthermore, the current study demonstrated that the partial prodomain encoded from exon 1 is critical for binding of MSTN-B to MSTN-A. Altogether, these data imply that alternative splicing isoforms of MSTN could negatively regulate pro-myostatin processing in muscle cells and prevent MSTN-mediated inhibition of myogenesis in avian species.

scholarly journals Mapping quantitative trait loci affecting fatness and breast muscle weight in meat-type chicken lines divergently selected on abdominal fatness

2006 ◽  
Vol 38 (1) ◽  
pp. 85 ◽  
Author(s):  
Sandrine Lagarrigue ◽  
Frédérique Pitel ◽  
Wilfrid Carré ◽  
Behnam Abasht ◽  
Pascale Le Roy ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Breast Muscle ◽  
Muscle Weight ◽  
Trait Loci ◽  
Abdominal Fatness ◽  
Meat Type

Lower skeletal muscle mass in male transgenic mice with muscle-specific overexpression of myostatin

2003 ◽  
Vol 285 (4) ◽  
pp. E876-E888 ◽  
Author(s):  
Suzanne Reisz-Porszasz ◽  
Shalender Bhasin ◽  
Jorge N. Artaza ◽  
Ruoqing Shen ◽  
Indrani Sinha-Hikim ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Transgenic Mice ◽  
Muscle Mass ◽  
Skeletal Muscle Mass ◽  
Fluorescent Protein ◽  
Male Mice ◽  
Wild Type ◽  
Specific Expression ◽  
Muscle Weight ◽  
Myostatin Gene

Mutations in the myostatin gene are associated with hypermuscularity, suggesting that myostatin inhibits skeletal muscle growth. We postulated that increased tissue-specific expression of myostatin protein in skeletal muscle would induce muscle loss. To investigate this hypothesis, we generated transgenic mice that overexpress myostatin protein selectively in the skeletal muscle, with or without ancillary expression in the heart, utilizing cDNA constructs in which a wild-type (MCK/Mst) or mutated muscle creatine kinase (MCK-3E/Mst) promoter was placed upstream of mouse myostatin cDNA. Transgenic mice harboring these MCK promoters linked to enhanced green fluorescent protein (EGFP) expressed the reporter protein only in skeletal and cardiac muscles (MCK) or in skeletal muscle alone (MCK-3E). Seven-week-old animals were genotyped by PCR of tail DNA or by Southern blot analysis of liver DNA. Myostatin mRNA and protein, measured by RT-PCR and Western blot, respectively, were significantly higher in gastrocnemius, quadriceps, and tibialis anterior of MCK/Mst-transgenic mice compared with wild-type mice. Male MCK/Mst-transgenic mice had 18–24% lower hind- and forelimb muscle weight and 18% reduction in quadriceps and gastrocnemius fiber cross-sectional area and myonuclear number (immunohistochemistry) than wild-type male mice. Male transgenic mice with mutated MCK-3E promoter showed similar effects on muscle mass. However, female transgenic mice with either type of MCK promoter did not differ from wild-type controls in either body weight or skeletal muscle mass. In conclusion, increased expression of myostatin in skeletal muscle is associated with lower muscle mass and decreased fiber size and myonuclear number, decreased cardiac muscle mass, and increased fat mass in male mice, consistent with its role as an inhibitor of skeletal muscle mass. The mechanism of gender specificity remains to be clarified.

Effect of kinin B2 receptor ablation on skeletal muscle development and myostatin gene expression

Neuropeptides ◽  
2010 ◽  
Vol 44 (2) ◽  
pp. 209-214 ◽  
Author(s):  
K. de Picoli Souza ◽  
E.C. Batista ◽  
E.D. Silva ◽  
F.C. Reis ◽  
S.M.A. Silva ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Muscle Development ◽  
Skeletal Muscle Development ◽  
Myostatin Gene

scholarly journals Influence of probiotic and prebiotic on meat quality of broiler chicken carcasses

2021 ◽  
Vol 32 ◽  
pp. 04009
Author(s):  
Irina Chervonova
Keyword(s):  
Meat Quality ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
First Grade ◽  
Control Group ◽  
Muscle Weight ◽  
The Third ◽  
The Impact ◽  
Positive Effect

The article presents the results of studying the impact of the spore-forming probiotic “Olin” and the prebiotic “Eсofiltrum” on the meat quality of broiler chicken carcasses of the Ross-308 cross. In the course of the research, it has been found that the inclusion of these preparations in the broiler diet has a positive effect on the studied indicators: the pre-slaughter weight increased by 4.3-5.7%, the weight of the gutted carcass by 4.6-6.2%, the muscle weight 4.8-6.5%, the weight of the loin muscles by 5.7-8.2%. The ratio of edible parts to inedible parts is 4.14 in the third experimental group, 4.08-in the second, and 4.04-in the control. Based on the results of the anatomical cutting of broiler carcasses and according to the fatness indicators the poultry carcasses of the third and second experimental groups, 87.5% and 87.2%, respectively, belong to the first grade, and the yield of the first grade carcasses is 86.2% in the control group. Meat products obtained from both the control poultry and the poultry of both experimental groups has no off-aroma odor and taste, the meat is juicy, tender and aromatic. The products obtained from broiler chickens, whose ration includes the probiotic “Olin” and the prebiotic “Ecofiltrum”, do not have any deviations from the established standards and norms. During the research, it has been found that the use of the studied drugs has a positive effect on the meat quality of poultry carcasses. However, broiler chickens receiving the probiotic “Olin” has more pronounced meat qualities.

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