scholarly journals The characteristics of fermented purple sweet potato (Ipomoea batatas) and black rice (Oryza sativa) using UV-irradiated Monascus purpureus

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
ISWIYANTI NOVITA ◽  
OEDJIJONO OEDJIJONO ◽  
ARI ASNANI

Abstract. Novita I, Oedjijono, Asnani A. 2021. The characteristics of fermented purple sweet potato (Ipomoea batatas) and black rice (Oryza sativa) using UV-irradiated Monascus purpureus. Biodiversitas 22: 684-690. This research aimed to produce Monascus fermented product (MFP) with purple sweet potato (Ipomoea batatas (L.) Lam.) and black rice (Oryza sativa L.) using UV-irradiated Monascus purpureus Went and evaluated the characteristic of its antibacterial activity against Salmonella typhi. M. purpureus was irradiated with UV at l254 nm for 0, 2, 3, and 4 min. The solid-state fermentation process was carried out for 7, 14, and 21 days. The pigments were measured at l390 nm for yellow and l500 nm for red. The ethanol extracts of MFP were analyzed for their antibacterial activity against S. typhi using the Kirby–Bauer method. The results showed that the highest yield of MFP was obtained from MFP–black rice (51.88%) that used UV-irradiated M. purpureus for 2 min and fermentation for 21 days. The highest absorbance value of MFP–purple sweet potato was obtained from UV-irradiated M. purpureus for 3 min, whereas the highest absorbance value of MFP–black rice was obtained with UV-irradiated M. purpureus for 2 min. Ethanol extracts of both MFP3–purple sweet potato and MFP2–black rice showed antibacterial activities against S. typhi with minimum inhibitory concentration values of 0.2 and 0.15 g/mL, respectively. Thin-layer chromatography analysis of the ethanol extract from MFP2–black rice revealed the presence of bioactive saponin and flavonoid. These findings suggest that UV-irradiated M. purpureus was able to use both purple sweet potato and black rice substrates to produce MFP with antibacterial activity against S. typhi.

2020 ◽  
Vol 4 (1) ◽  
pp. 14-23
Author(s):  
Isana Supiah Yosephine Louise

AbstrakInulin merupakan bahan dasar obat yang banyak terkandung dalam umbi-umbian lokal, seperti ubi kayu/singkong (Manihot esculenta), uwi (Dioscorea spp.), ubi jalar (Ipomoea batatas), dan gembili (Dioscorea esculenta L.), yang secara luas belum diupayakan untuk diproduksi secara besar-besaran. Umbi-umbian lokal umumnya hanya dijual sebagai bahan mentah yang harganya relatif murah dan tidak dapat disimpan dalam waktu yang relatif lama. Umumnya dikonsumsi dengan cara direbus, digoreng atau diolah menjadi makanan tradisional, belum diproses menjadi produk yang lebih bermanfaat, memiliki nilai jual relatif tinggi, dan waktu penyimpanan yang relatif lebih lama. Pada kegiatan pemberdayaan masyarakat pedesaan ini telah dilakukan penyuluhan, pemberian keterampilan, dan pendampingan masyarakat dusun Krajan Wedomartani Ngemplak Sleman untuk secara mandiri mampu mengolah umbi-umbian lokal menjadi produk tepung fermentasi, tepung non fermentasi, dan ekstrak inulin. Pada kegiatan ini bahan baku yang digunakan adalah umbi singkong, uwi ungu, dan ubi jalar ungu. Proses fermentasi umbi dilakukan menggunakan jamur monascus angka atau monascus purpureus. Ekstrak inulin dilakukan dengan menggunakan pelarut alkohol. Produk tepung fermentasi dengan bahan baku umbi singkong, uwi ungu, dan ubi jalar ungu masing-masing memiliki randemen sebesar 52%, 43%, dan 40%; sedangkan ekstrak inulin memiliki randemen sesesar 7,47%, hanya saja produk inulin belum optimum bila ditinjau dari sisi warna senyawa, masih perlu dilakukan proses lanjutan untuk memperoleh produk inulin yang sesuai standar pasar. Hasi angket menunjukkan bahwa sebagian besar peserta menyatakan kegiatan seperti ini sangat bermanfaat, dan secara umum penyajian materi oleh tim pengabdi dapat diterima dengan mudah dan jelas oleh peserta. Sebagian besar peserta menyatakan bahwa metoda penyampaian materi sesuai, artinya, secara umum metoda penyampaian materi dalam kegiatan ini dapat diterima dengan mudah oleh masyarakat sasaran. Sebagian besar peserta menyatakan bahwa kegiatan seperti ini sangat membantu membuka wawasan masyarakat desa untuk dapat mengolah umbi-umbian lokal, yang semula hanya dikonsumsi dengan direbus atau digoreng atau dijual mentah dengan harga realtif murah menjadi produk yang lebih bermanfaat, memiliki nilai jual relatif tinggi, dan memiliki daya simpan relatif lebih lama. Kata kunci: inulin, monascus angka, umbi-umbian lokal, membuka wawasan, masyarakat desa The Production of Tubers-Based Inulin as The Basic Ingredients of Medicine AbstractInulin is a raw material of medicine which is contained in local tubers, such as cassava (Manihotes culenta), Dioscorea spp., sweet potato (Ipomoea batatas L.), and Dioscorea esculenta L., which is not widely strived in mass production. Local tubers commonly sold as food raw material which is relatively cheap and cannot be stored for a long time. Generally, the tubers consumed by boiling, fried or processed to traditional food. It has not processed to be more valuable product, has relatively high price, and has relatively longer the storage time. In the empowerment activity of rural community, it has been conducted counseling, providing soft skill, and community assistance in Krajan, Wedomartani, Ngemplak, Sleman to independently be able producing local tubers became fermented flour, unfermented flour, and inulin extract product. In this activity, the raw material were cassava, Dioscorea alata L. syn. D. atropurpurea Roxb., and purple sweet potato (Ipomoea batatas var Ayumurasaki). Tubers fermentation process was conducted using monascus angka or monascus purpureus mold. Inulin extract was produced using alcohol solvent. Fermented flour product from cassava, Dioscorea alata L. syn. D. atropurpurea Roxb., and purple sweet potato have yield as respectively 52%, 43%, and 40%; meanwhile inulin extract has yield as 7.47%, although inulin product had not reach optimum point regarding on the color of compound. It still needs further process to gain standardized commercially inulin product. Questioners showed that mostly of the attendances stated the activity very useful, and generally the presentation from empowerment team can be delivered easily and clearly to the communities.  Mostly of the attendances stated that the method of presentation is suitable, which means generally the presentation method in this activity can be accepted easily by the communities. Mostly of the attendances stated that the activity is very helpful for them to gain the knowledge of local tubers processing, which was originally consumed only with boiled or fried or sold uncooked with the relatively cheap price to be more valuable product, has relatively high price, and has relatively longer the storage time  Key words: inulin, monascus angka, local tubers, empowerment, rural community


PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 487
Author(s):  
Grace Dien Manik ◽  
Hosea J. Edy ◽  
Elly J. Suoth

ABSTRACT Purple Sweet Potato (Ipomoea batatas L.) and Red Cana Flower (Canna coccinea Mill.) contain natural dyes (anthocyanins) which are used as an alternative natural dyes as well as natural antioxidants. This study aims to make and discover the physical evaluation of blusher preparations using natural dyes in a combination of Purple Sweet Potato extract and Red Cana Flower with a concentration of 15%: 15%, 15%: 20 %, 20%: 20%. This research used laboratory experimental methods. The results of the physical properties test showed that the resulting preparations gave a light purple to purplish pink color, had a distinctive odor of pafrum oleum rose, had a soft texture, all preparations had a homogeneous composition, the ph value of the preparations ranged from 6.00 – 6.45, the best polish at concentrations dye 20%: 20%, for 14 days of observation the color on all preparations did not change with light, and the most preferred preparations by researcher are those with dye concentrations of 20%: 20%. It shows that it can be concluded that a combination of ethanol extract of Purple Sweet Potato and Red Cana Flower. Can be used as a natural dye in blush preparations. Keywords:  Ipomoea batatas L., Canna coccinea Mill., Blusher. ABSTRAK  Ubi Jalar Ungu (Ipomoea batatas L.) dan Bunga Kana Merah (Canna coccinea Mill.) mengadung pewarna alami (antosianin) yang digunakan sebagai zat pewarna alami alternatif mapun sebagai antioksidan alami. Penelitian ini bertujuan untuk membuat serta mengetahui evaluasi fisik sediaan perona pipi dengan memanfaatkan pewarna alami pada kombinasi ekstrak Ubi Jalar Ungu dan Bunga Kana Merah dengan konsentrasi 15%:15%, 15%:20% dan 20%:20%. Penelitian ini menggunakan metode eksperimental laboratorium. Hasil pengujian sifat fisik menunjukan bahwa sediaan yang dihasilkan memberikan warna ungu muda hingga merah muda keunguan, bau khas parfum oleum rose, teksturnya lembut, semua sediaan memiliki susunan yang homogen, nilai pH sediaan berkisar 6,00 - 6,45, polesan terbaik pada konsentrasi zat warna 20%:20%, selama 14 hari pengamatan warna pada semua sediaan tidak mengalami perubahan terhadap cahaya, dan sediaan yang paling disukai oleh panelis adalah sediaan dengan konsentrasi zat warna 20%:20%. Dengan demikian dapat disimpulkan bahwa kombinasi ekstrak etanol Ubi Jalar Ungu dan Bungu Kana Merah dapat digunkan sebagai pewarna alami dalam sediaan perona pipi. Kata kunci: Ipomoea batatas L., Canna coccinea Mill., Perona pipi


2019 ◽  
Vol 18 (9) ◽  
pp. 824-833
Author(s):  
A.F. Khairani ◽  
T. Nurhayati ◽  
P.H.A. Rahman ◽  
Nurhasanah . ◽  
R. Khaerunnis ◽  
...  

2020 ◽  
Vol 6 (2) ◽  
pp. 252
Author(s):  
Herman Irawan ◽  
Sevty Syera ◽  
Nurlaili Ekawati ◽  
Djadjat Tisnadjaja

Papaya (Carica papaya L.) and purple sweet potato (Ipomoea batatas L. Lam) are commonly used empirically as traditional medicines, including for malaria, malnutrition, fever and hemorrhagic fever. The purpose of this study was to determine the effect of differences in the concentration of ethanol solvents on the chromatogram profile and compound content. The research method began with maceration using 50%, 70%, and 96% ethanol, then thin layer chromatography test, and determination of total phenol and flavonoid levels with Elisa at λ of 750 nm and 415 nm, where the comparator used were gallic acid and quercetin . The results of total phenol levels obtained in papaya leaf extract were 3,493 mg GAE/gram and in sweet potato leaves the results were 4,786 mgGAE/mg. While the total flavonoid yield obtained from papaya leaf extract was obtained as much as 4,630 mg QE/gram and on sweetpotato which was 4,269 mgQE/mg. Characterisation of extract compound content was carried ouy by using Gas Chromatography- Mass Spectroscopy (GC-MS), where comparison of extracts used in extract combination samples are 50:50, 75:25, and 25:75. The results showed that ethanol extract contained alkaloids, flavonoids, saponins, tannins, and triterpenoids. Characterization by using GC-MS for single extract and combination extract of papaya leaves and purple sweet potato leaves obtained the main active compounds are Phytol, Neoheptadine, and n-Hexadecanoic acid.


Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3812
Author(s):  
Peerachit Tonchaiyaphum ◽  
Warangkana Arpornchayanon ◽  
Parirat Khonsung ◽  
Natthakarn Chiranthanut ◽  
Pornsiri Pitchakarn ◽  
...  

Black rice is a type of rice in the Oryza sativa L. species. There are numerous reports regarding the pharmacological actions of black rice bran, but scientific evidence on its gastroprotection is limited. This study aimed to evaluate the gastroprotective activities of black rice bran ethanol extract (BRB) from the Thai black rice variety Hom Nil (O. sativa L. indica) as well as its mechanisms of action, acute oral toxicity in rats, and phytochemical screening. Rat models of gastric ulcers induced by acidified ethanol, indomethacin, and restraint water immersion stress were used. After pretreatment with 200, 400, and 800 mg/kg of BRB in test groups, BRB at 800 mg/kg significantly inhibited the formation of gastric ulcers in all gastric ulcer models, and this inhibition seemed to be dose dependent in an indomethacin-induced gastric ulcer model. BRB could not normalize the amount of gastric wall mucus, reduce gastric volume and total acidity, or increase gastric pH. Although BRB could not increase NO levels in gastric tissue, the tissue MDA levels could be normalized with DPPH radical scavenging activity. These results confirm the gastroprotective activities of BRB with a possible mechanism of action via antioxidant activity. The major phytochemical components of BRB comprise carotenoid derivatives with the presence of phenolic compounds. These components may be responsible for the gastroprotective activities of BRB. The 2000 mg/kg dose of oral BRB showed no acute toxicity in rats and confirmed, in part, the safe uses of BRB.


Author(s):  
I Wayan Putu Sutirta-Yasa ◽  
I Made Jawi ◽  
Ida Bagus Ngurah ◽  
Anak Agung Ngurah Subawa

Oxidative stress as a consequence of chronic alcoholic intake causes lesions of liver tissue. The objective of this study was to identifythe antioxidant effect of ethanol extract derived from Balinese purple sweet potato on the liver tissue in mice after chronic consumptionof alcohol. Subjects of this study were 32 adults male Swiss mice (12–14 weeks) divided into 4 groups with control group post-testonly design. Post-treatment of blood and liver samples were collected from each group of 8 mice. The MDA liver was quantified withthiobarbituric acid reactive substances (TBARS) method. Samples were collected from control group of 8 mice without sweet potatoextract and alcohol, 8 mice with 1 mg extract of Balinese purple sweet potato, 8 mice with 0.8 gram alcohol, and 8 mice with combineof alcohol and extract of Balinese purple sweet potato, each treatment was carried out every day for 4 weeks. The results showed asignificant increase of serum SGOT, SGPT and liver MDA level, after chronic consumption of alcohol (p = 0.00). The decrease of serumSGOT, SGPT and liver level MDA was significant in the group with extract of Balinese purple sweet potato (p = 0.00). The researchersconcluded that the extract of Balinese purple sweet potato had an antioxidant effect in mice which consumed alcohol chronically. Basedon this study it is concluded that extract of Balinese purple sweet potato has a protective effect on the liver cell in mice which consumedalcohol chronically.


2021 ◽  
Vol 6 (2) ◽  
pp. 69-73
Author(s):  
Damaranie Dipahayu ◽  
Kinanti Ayu Puji Lestari

Ethanol extract of purple sweet potato (Ipomoea batatas (L.)) leaves of Antin-3 variety contains flavonoids and polyphenols which can be used as a source of acne anti-bacterial because it can inhibit the growth of Staphylococcus aureus. This study aims to obtain data on physical characteristics, acceptability and stability of anti-acne mask formulation of 70% ethanol extract of Antin-3 leaves. The research method used were characteristic, acceptability and stability test. There were 4 types of samples tested, namely base, Antin-3 extract 5%: 10% and 20% (B: F1: F2: F3). The organoleptic results showed that the higher concentration of  extract, the darker color of mask and the stronger of extract smell and the softer of consistency. The pH values ​​(B: F1:F2: F3) were respectively 6.46; 5.78; 5.75; 5.46. The value of adhesion (B: F1: F2: F3) were respectively 1.1; 1.46; 2.17 and 4.53 seconds. The value of spreading capacity (B: F1: F2: F3) were respectively 3.89; 4.15; 4.36 and 3.45 cm. The results of the acceptability test showed that F2 was the easiest formula to spread, clean and  softest sensation on the skin. The results of the mechanical  and cycling test showed that the four samples had not undergo phase separation. Anti-acne mask with 70% ethanol extract of Antin-3 leaves by 10% met the physical characteristics, acceptability and stability.


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