scholarly journals Methanol pigment extracts derived from two marine actinomycetes exhibit antibacterial and antioxidant activities

2021 ◽  
Vol 22 (10) ◽  
Author(s):  
DOVA KELVIN MESRIAN ◽  
WIDYA ESTI PURWANINGTYAS ◽  
RIKA INDRI ASTUTI ◽  
AKHMAD ENDANG ZAINAL HASAN ◽  
Aris Tri Wahyudi
Keyword(s):  
Crude Extract ◽  
Antioxidant Activities ◽  
Rrna Gene ◽  
Marine Actinomycetes ◽  
Marine Microorganisms ◽  
E Coli ◽  
Crude Extracts ◽  
Gene Sequence Analysis ◽  
Ic50 Values ◽  
Pigment Extract

Abstract. Mesrian DK, Purwaningtyas WE, Astuti RI, Hasan AEZ, Wahyudi AT. 2021. Methanol pigment extracts derived from two marine actinomycetes exhibit antibacterial and antioxidant activities. Biodiversitas 22: 4440-4447. In recent years, natural pigments produced by marine microorganisms, especially actinomycetes, have become an important source of medically valuable drugs and other active compounds. Based on that crucial fact, this study aimed to determine the toxicity, antimicrobial, and antioxidant activities of methanolic pigment extract derived from marine actinomycetes HV11.P3 and SCA54.P2. Both extracts exhibited high toxicity activity based on Brine Shrimp Lethality Test (BSLT). The results showed the lethal concentration 50% (LC50) values of actinomycetes HV11.P3 and SCA54.P2 pigment crude extracts were 94.60 µg/mL and 131.22 mg/mL, respectively. The antimicrobial activity of those extracts was also tested against several microbes and showed the best results for inhibiting against Bacillus subtilis and Escherichia coli. Furthermore, we assessed the antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and the results showed the inhibitory concentration 50% (IC50) values ??of actinomycetes HV11.P3 and SCA54.P2 pigment crude extracts were 231.08 µg/mL and 369.3 µg/mL, respectively. By using GC-MS analysis, we identified chemical compounds of actinomycete pigment crude extracts, revealing that the most dominant compounds were 1H-Purine-2,6-dione, 3,7-dihydro-1,3,7-trimethyl- (CAS) (44.65%) for HV11.P3 and 5-Methoxypyrrolidin-2-one (30.23%) for SCA54.P2. The 16S rRNA gene sequence analysis showed that actinomycete HV11.P3 and actinomycete SCA54.P2 had the closest similarity to Micromonospora chalcea strain 1464-217L and Micromonospora tulbaghiae strain TVU1, respectively. This study revealed that two pigment crude extracts derived from actinimycetes HV11.P3 and SVA54.P2 had antibacterial, antioxidant, and toxicity activities. The actinomycete SCA54.P2 pigment crude extract had the best activity as an antibacterial against E. coli ATCC 8739, while actnomycete HV11.P3 pigment crude extract had the best activity as an antioxidant.

scholarly journals In Vitro Antioxidant and Antimicrobial Activities of Ephedra gerardiana (Root and Stem) Crude Extract and Fractions

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Aman Khan ◽  
Gul Jan ◽  
Afsar Khan ◽  
Farzana Gul Jan ◽  
Ali Bahadur ◽  
...  
Keyword(s):  
Crude Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Aqueous Fraction ◽  
Oxidative Stresses ◽  
Crude Extracts ◽  
Stem Extract

The utilization of medicinal plants to treat infectious disease is a common practice in developing countries worldwide. The present study was aimed at evaluating the crude extracts of Ephedra gerardiana (root and stem) with different chemicals for antioxidant and antimicrobial (fungal and bacterial) potential. The results revealed that the ethyl acetate fractions of E. gerardiana (root and stem) have significant free radical scavenging potential with values 2.96±0.39 and 2.73±0.84 while n-butanol and aqueous fractions showed IC502.69±0.26 and 3.44±0.69 µg/ml in stem. Furthermore, crude extract and fractions also revealed promising antibacterial activities against all tested microbial strains while aqueous fraction showed no activities against Bacillus subtilis, Kleibsiella pneumoniae, and Pseudomonas aeruginosa. Interestingly, all crude extracts and fractions were nonactive against fungal strain, Aspergillus niger and Aspergillus flavus, as compare to control. In summary, the Ephedra gerardiana (root and stem) extract and fraction possess antioxidant activities, which might be helpful in preventing or slowing the progress of various oxidative stresses, suggested to be a strong pharmaceutical agent.

scholarly journals EKSTRAKSI DAN PENGAMATAN AKTIVITAS ANTIOKSIDAN DAN ANTIMIKROBA DARI KULIT BUAH POMETIA PINNATA

2017 ◽  
Vol 11 (2) ◽  
pp. 80 ◽  
Author(s):  
Fransisca C. Faustina ◽  
Filiana Santoso
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Crude Extract ◽  
Inhibitory Concentration ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Fruit Peel ◽  
Crude Extracts

<p align="justify">Studi ini meneliti ekstraksi kulit buah matoa (Pometia pinnata), buah dari famili Sapindaceae. Ekstraksi dilakukan dengan teknik maserasi menggunakan 3 pelarut: aseton, etanol dan akuades. Ekstrak tersebut kemudian dievaluasi aktivitas antioksidan dan antimikrobanya. Tes antimikroba menggunakan tes difusi lempeng dan difusi cairan. Aktivitas antioksidan tertinggi dihasilkan oleh ekstrak aseton dengan nilai IC50 15.323 ppm, kemudian ekstrak etanol 143.23 ppm dan 451.306 ppm. Aktivitas antioksidan dari ketiga ekstrak tersebut dibandingkan dengan aktivitas antioksidan dari asam askorbat dan ekstrak aseton menunjukkan kekuatan antioksidan setara dengan 50% dari kekuatan antioksidan dari asam askorbat. Aktivitas anti mikroba dievaluasi terhadap Escherichia coli, Bacillus cereus dan Staphylococcus aureus. Hasil evaluasi menunjukkan bahwa semua ekstrak memiliki aktivitas antimikroba dengan karakter bakteriostatis. Karena itu nilai NIC (Non-inhibitory Concentration) ditetapkan untuk semua ekstrak. Aktivitas tertinggi ditunjukkan oleh ekstrak aseton dan etanol dengan NIC &lt;0.5 ppm terhadapsemua bakteri tes, sementara aktivitas terlemah ditunjukkan oleh ekstrak akuades dengan NIC 5 ppm. Pengukuran kadar fenolik dari semua ekstrak dilakukan dengan tes Folin-Ciocalteau, dan ditemukan adanya korelasi positif antara kadar fenolik dan aktivitas antioksidan dari semua ekstrak. Analisa komponen dengan tes tannin, saponin dan spektrometri juga dilakukan dan hasil menunjukkan adanya kandungan tannin, saponin dan alkaloid di dalam kulit buah matoa.</p><p><strong>Kata kunci :</strong>Matoa, Pometia pinnata, Ekstraksi, Antimikroba, Antioksidan</p><hr /><p align="center"><strong>English Version Abstract</strong></p><p align="justify">This study investigated the extraction of matoa (Pometia pinnata) fruit peels, a fruit in the family of Sapindaceae. The extraction was performed through maceration method using three kinds of solvent: acetone, ethanol and water. The extracts were tested for their antioxidant and antimicrobial activities. DPPH radical scavenging assay was used in evaluating the antioxidant activities. The antimicrobial activity was evaluated using disc-diffusion and broth dilution method. The highest antioxidant activity was showed by acetone crude extract with IC50 value at 15.323 ppm, followed by ethanol and water crude extract at 143.23 ppm and 451.306 ppm, respectively. The antioxidant activities of the crude extracts were compared to L-ascorbic acid and it was found that acetone crude extract exhibited half the strength of the antioxidant activity of L-ascorbic acid. Antimicrobial activity of matoa fruit peel extracts were tested against Escherichia coli, Bacillus cereus and Staphylococcus aureus. The results showed that all crude extracts showed antimicrobial activity with bacteriostatic characteristic. Therefore, Non-Inhibitory Concentration (NIC) was determined for all crude extracts. Acetone and ethanol crude extract possessed the strongest antimicrobial activity at NIC &lt;0.5 ppm against all tested microorganisms, while water crude extract showed the weakest antimicrobial activity with NIC at 5 ppm. The total phenolic content of the crude extracts were measured using Folin-Ciocalteau method. Positive correlation between the antioxidant activities and the total phenolic content of the crude extracts was detected. Saponin and tannin test, as well as spectrometric analyses supported the presence of saponin, tannin and alkaloid in matoa fruit peel.</p><p><strong>Keywords :</strong>Matoa, Pometiapinnata, Extraction, Antimicrobial, Antioxidant</p>

scholarly journals Antioxidant and Anti-Inflammatory Activities of the Crude Extracts of Moringa oleifera from Kenya and Their Correlations with Flavonoids

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 296 ◽  
Author(s):  
Yong-Bing Xu ◽  
Gui-Lin Chen ◽  
Ming-Quan Guo
Keyword(s):  
Moringa Oleifera ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Reducing Power ◽  
No Production ◽  
Antioxidant Power ◽  
Crude Extracts ◽  
Ic50 Values ◽  
Anti Inflammatory

Moringa oleifera Lam. (M. oleifera) is commonly distributed and utilized in tropical and sub-tropical areas. There has been a large number of reports on the antioxidant and anti-inflammatory activity of its leaves, but only a few about its seeds and roots. Hence, in this work we aimed to systematically compare the antioxidant and anti-inflammatory activities of the ethanol crude extracts of leaves, seeds, and roots of M. oleifera from Kenya, and further correlate the differential activities with the chemical constituents from these three parts. The antioxidant activities were measured by using three different assays (DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) and FRAP (Ferric-Reducing Antioxidant Power), respectively). Results showed that the leaf extracts displayed the highest DPPH radical scavenging and FRAP total reducing power activities with IC50 values of 1.02 ± 0.13 mg/mL and 0.99 ± 0.06 mM Fe2+/g, respectively; the leaf and root extracts exhibited potential ABTS radical scavenging activities with the IC50 values of 1.36 ± 0.02 and 1.24 ± 0.03 mg/mL. Meanwhile, the leaf and seed extracts (11.1–100 µg/mL) also exerted obvious anti-inflammatory activities, as indicated by the inhibition of NO production. To further reveal correlations between these differential activities with the chemical constituents in the three organs, the total flavonoids content (TFC) of the three different extracts were evaluated, and the TFC of leaves, seeds and roots were found to be 192.36 ± 2.96, 5.89 ± 0.65 and 106.79 ± 2.12 mg rutin equivalent (RE)/g, respectively. These findings indicated the important impacts of the total flavonoid contents on antioxidant and anti-inflammatory activities. Additionally, we further determined the phytochemical profiles of M. oleifera by HPLC-UV/ESI-MS/MS, and identified most of the chemical constituents of leaves as flavonoids. In summary, the leaves of M. oleifera are a better potential natural source of antioxidants and anti-inflammatory agents, and very promising for development into the health promoting dietary supplements.

scholarly journals Antimicrobial and Antioxidant Activities of Streptomyces sps Isolated from Muthupettai Mangrove Soil

2021 ◽  
pp. 210-234
Author(s):  
C. Nivetha ◽  
T. Deepika ◽  
A. Arjunan ◽  
P. Sivalingam ◽  
N. Revathi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Rrna Gene ◽  
Mangrove Soil ◽  
Soil Sediment

Muthupet mangrove forest soil sediment was the abundant resource of the actinomycetes with distinct nature of bioactive compounds. The soil sediment was collected at 1-3meter away from bank. The present study was focused on isolation, identification and antimicrobial activity of the actinomycetes from Muthupet mangrove soil samples. Totally 32 actinomycetes strains was isolated and screened for antimicrobial activity against bacterial and fungal pathogens. Among 32 isolates 16 have antibacterial activity and 10 have antifungal activity but MG-3 and MG-4 showed maximum activity against both all the test pathogens. These two strains are gram-positive, rod-shaped, MG-3 possessing an earthy characteristic odour and MG-4 produce purple color pigment. The isolates were confirmed as Streptomyces sp. based on morphological, cultural, biochemical and physiological observations, as well as identification using the 16S rRNA gene sequence, it showed 98% similarity with Streptomyces parvus for MG-3 and Streptomyces californicus for MG-4. Bioactive compounds were extracted from Streptomyces using different solvents such as ethyl acetate, methanol, chloroform, hexane and antibacterial activities were assayed against test pathogens, ethyl acetate extract showed maximum zone of inhibition when compared with other solvents. The Minimum inhibitory concentration of ethyl acetate extract was found ranged between 1.96-3.9 μg/ml. The invitro antioxidant capacity of the crude extract was estimated by DPPH, ferric reducing power assay, H202 radical scavenging assay, phosphomolybdenum assay and total antioxidant activities. The characterization of crude extracts was analyzed by FTIR and GC-MS. From the results, it is clear that the ethyl acetate crude extract of S.parvus MG-3 and S.californicus MG-4 possesses high antimicrobial and antioxidant activity and suggested that the isolated strains could be a potential for the nature resource of pharmaceutical.

scholarly journals Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia

2019 ◽  
Vol 14 (2) ◽  
pp. 73
Author(s):  
Wendy Alexander Tanod ◽  
Didit Kustantio Dewanto ◽  
Samliok Ndobe ◽  
Putut Har Riyadi ◽  
Masteria Yunovilsa Putra
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Moderate Activity ◽  
Soft Corals ◽  
Strong Activity ◽  
E Coli ◽  
Central Sulawesi

This study aimed to evaluate the potential antibacterial and antioxidant activities of Sinulariasp. and Sarcophyton sp. from the Palu Bay, Central Sulawesi, Indonesia. Soft corals were identified as Sinulariasp. (SC1), Sinularia sp. (SC2), andSarcophytonsp. (SC3). Antibacterial activity was examined using agar diffusion well method. Antioxidant activity was measured by the DPPH radical scavenging method. The samples were macerated in MeOH: DCM. The crude extracts were partitioned with DCM, EtOAc, and BuOH. The crude extract of Sinulariasp. (SC2) showed a very strong antibacterial activity as it was able to inhibit the growth of Staphylococcus aureusand Escherichia coliup to 10 mg/mL. Sinularia sp. (SC1) crude extract showed strong activity againstS. aureus, whereas it showed moderate against E. coli.Sarcophyton sp. (SC3) crude extract showed moderate activity against S. aureus, whereas it showed weak against E. coli. The partition fractions of the three soft coral extracts had the potential to be a potent antioxidant agent.

scholarly journals UJI ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS TERHADAP Escherichia coli DAN Staphylococcus aureus SERTA POTENSINYA TERHADAP AKTIVITAS ANTI-UV

2019 ◽  
Vol 7 (3) ◽  
pp. 196
Author(s):  
Monika Gabrielle ◽  
Deiske A Sumilat ◽  
Veibe Warouw ◽  
Remy EP Mangindaan ◽  
Chatrien AC Sinjal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Diffusion Method ◽  
Water Fraction ◽  
E Coli ◽  
Self Defense ◽  
Crude Extracts ◽  
Water Fractions

Sponge organisms produce bioactive compounds that are toxic as a means of self-defense. The compound is known to have the potential as an antibacterial and anti-UV which can absorb sunlight with the potential to be used as a material for making sunscreen. The purpose of this study was to obtain antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and anti-UV activity from crude extracts and sponges fractions. The antibacterial test done by agar diffusion method (Kirby and Bauer diffusion disc) and the crude extract and the active fraction of antibacterial compounds were tested in a UV spectrophotometer to see its anti-UV activity. As a result, 4 species of sponges were extracted and partitioned into water fractions, methanol fractions, and n-hexane fractions. All samples were tested for antibacterial activity and the results showed antibacterial activity against S. aureus by Plakortis sp. in crude extract (9 mm) and water fraction (8.6 mm), Agelas sp. in crude extract (7 mm) and in E. coli bacteria shown by Plakortis sp. in crude extract (12.6 mm) and water fraction (9 mm), Liosina sp. in the water fraction (7.6 mm), Haliclona sp. in the water fraction (8 mm) and Agelassp. in crude extract (10.3 mm). Crude extracts and water fractions were tested using a UV spectrophotometer for anti-UV testing, the results showed that crude extract and all the water fractions of four species sponge could absorb UV-B (λ 290-320 nm) and UV-A (λ 320-400 nm).Keywords: sponges, antibacterial, partition, anti-UV

scholarly journals Screening, in-vitro propagation and bioaugmentation of Ceratotheca triloba for the production of secondary metabolites

2010 ◽  
Author(s):  
◽  
Viresh Mohanlall
Keyword(s):  
Synergistic Effect ◽  
Traditional Medicine ◽  
Cell Suspension ◽  
Crude Extract ◽  
Hepg2 Cells ◽  
Topoisomerase Ii ◽  
Inflammatory Activity ◽  
E Coli ◽  
Crude Extracts ◽  
Anti Cancer

Ceratatheca triloba (Bernh.) E. Mey. Ex Hook. f. is one of four species that is common to the summer rainfall areas in South Africa, especially the grasslands. It is used in traditional medicine to treat stomach cramps, nausea, fever and diarrhea. Like many other plants used in the traditional medicine system, these uses are not justified through scientific investigations. This study was undertaken to characterize the functionality of the main bioactive compounds from Ceratatheca triloba. This was achieved by isolating and identifying predominant chemicals from the non polar extracts using conventional chromatography techniques. Once identified the crude extracts and identified compounds were tested for their antimicrobial, anti-oxidant activity, anti-inflammatory activity and anticancer activity. This was followed by investigating the safety of the crude extracts and the purified compounds by the Brine shrimp lethality assay, and its toxicity to HepG2 cells and the Salmonella mutagenecity test. For large scale production, we set up a protocol to produce 9, 10 anthracenedione in a cell suspension culture system. Following the complete chemical profile of the roots, stems, flowers and leaves the predominant compounds were isolated, characterized and identified by UV-Vis, IR, EI-LCMS and NMR (COSY, HMQC, HMBC and DEPT). Three anthraquinone derivatives and one steroid, 9, 10 anthracenedione, 1-hydroxy-4-methylanthraquinone, 5, 8-dimethoxy-2, 3, 10, 10a-tetrahydro-1H-phenanthrene-4, 9-dione and androst-5-ene-3, 17, 19-triol were determine by analysis of spectral data (UV, 1H NMR, 13C NMR and EI-LC-MS) 9, 10 anthracenedione and 1 hydroxy-4-methylanthraquinone showed antibacterial activity against S.aureus, M. luteus, B cureus and E. coli. Due to the synergistic effect of the individual compounds, the crude extract exhibited good potency (>500) against S.aureus and M. luteus, medium potency against E. coli. and S. typhimurium (<100) and very low potency against B cureus (<10). Although a similar trend was observed for 9, 10 anthracenedione and 1 hydroxy-4-methylanthraquinone unlike the crude extract. A very low potency against S.aureus for 9, 10 anthracenedione and a high potency for 1 hydroxy-4-methylanthraquinone. Thus 9, 10 anthracenedione is an effective drug against E. coli and S. typhimurium and 1 hydroxy-4-methylanthraquinone is effective against S.aureus and M. luteus. The crude root extracts and 9, 10 anthracenedione, 1 hydroxy-4-methylanthraquinone, 8-dimethoxy-2, 3, 10, 10a-tetrahydro-1H-phenanthrene-4 showed a ± 50% reduction of the free radicals. No anti-inflammatory activity was observed. The purified extracts showed moderate toxicity against HepG2 cells at high concentrations and no toxicity was observed against brine shimp larvae. No mutagenecity was observed with the crude extracts using the Ames test. All purified and crude extracts showed potent inhibition of the human topoisomerase II enzyme. In conclusion, although this study does not indicate any relationship to its traditional usage it provides valuable information that paves a way for commercial exploitation of C. triloba. 9, 10 anthracenedione and 1 hydroxy-4-methylanthraquinone can be used as antibacterial agents. Their antioxidative potential can be exploited for anti-cancer as in many cancers reactive oxygen species are implicated in the aetiology of these cancers. Furthermore, in this study 9, 10 anthracenedione was produced from both callus cultures and cell suspension cultures. This compound demonstrates potent anti-topoisomerase II activity which is vital to cancer treatment. Thus, the synergistic effect of 9, 10 anthracenedione and 1 hydroxy-4-methylanthraquinone as antibacterial, anti-oxidative and anti-cancer compounds demonstrate the importance of C. triloba.

scholarly journals Aporphine Alkaloids of Cinnamomum mollissimum and their Bioactivities

2014 ◽  
Vol 9 (1) ◽  
pp. 1934578X1400900
Author(s):  
Fatin Fasihah Masnon ◽  
Najmah PS Hassan ◽  
Farediah Ahmad
Keyword(s):  
Spectral Analysis ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Chemical Investigation ◽  
Crude Extracts

A chemical investigation of the crude extracts of Cinnamomum mollissimum bark resulted in the isolation of five aporphine alkaloids, namely N-methyl-1,2,10-trimethoxyaporphine, N-methylhernagine, N-methylhernovine, hernagine and hernovine. Their structures were elucidated by spectral analysis. The alkaloid crude extract demonstrated significant antibacterial and antioxidant activities.

scholarly journals Antibacterial and Antioxidant Activity of Rhodomyrtus Tomentosa and Cinnamomum Zeylanicum Crude Extracts

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Tran Thi Quynh Lan ◽  
Vu Manh Khiem ◽  
Nguyen Van Tin
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Inhibition Zone ◽  
Antioxidant Compounds ◽  
Crude Extracts ◽  
Layer Chromatography ◽  
Antibacterial And Antioxidant Activity ◽  
Rhodomyrtus Tomentosa

The aim of this  study was to investigate the extraction method for R. tomentosa and C. zeylanicum leaves and the evaluation of antibacterial and antioxidant activities of crude extracts. The results of the study showed that the active ingredients of crude extracts were  clearly separated by Thin-layer chromatography and the presence of rhodomyrtone in R. tomentosa crude extract and cinnamaldehyde in C. zeylanicum crude extract. R. tomentosa crude  extract was  antibacterial activity against Staphylococcus aureus with 13.1 mm of inhibition zone, but is not effective against Salmonella Typhimurium. C. zeylanicum leaf extract did not show antibacterial activity on both S. aureus and S. Typhimurium. At a dilution of 1/2 of the R. tomentosa crude extract can completely inhibit S. aureus growth. This study also indicated the presence of antioxidant compounds such as flavonoids, tannins, phenols and terpenoids in C. zeylanicum and R. tomentosa crude extracts. The results showed that R. tomentosa and C. zeylanicum crude extracts should be used as a biotherapy alternative to antibiotic therapy. However, further study would be needed to investigate the antibacterial activity of crude extracts in vivo. 

Metabolomics and Pharmacological Screening of Aspergillus versicolor Isolated from Hyrtios erectus Red Sea Sponge; Egypt

2020 ◽  
Vol 16 (7) ◽  
pp. 1083-1102
Author(s):  
Mohamed A. Shreadah ◽  
Nehad M.A. El Moneam ◽  
Samy A. El-Assar ◽  
Asmaa Nabil-Adam
Keyword(s):  
Oxidative Stress ◽  
Red Sea ◽  
Crude Extract ◽  
Quantitative Methods ◽  
Total Phenolic ◽  
Aspergillus Versicolor ◽  
Crude Extracts ◽  
Study Results ◽  
Anti Inflammatory ◽  
Pharmacological Screening

Background: Aspergillus Versicolor is a marine-derived fungus isolated from Hyrtios Erectus Red Sea sponge. Methods: The aim of this study was to carry out a pharmacological screening and investigation for the in vitro biological activity (antioxidant, cholinergic, antidiabetic and anticancer) of Aspergillus Versicolor crude extract’s active compounds by using different qualitative and quantitative methods. Results: The present study results showed that Aspergillus Versicolor crude extracts contain 0.6 mg total phenolic/mg crude extract. Aspergillus Versicolor also showed a potent antioxidative capacity by decreasing the oxidation of ABTS. The anticancer and inhibitory effects of Aspergillus Versicolor crude extracts on PTK and SHKI were found to be 75.29 % and 80.76%; respectively. The AChE inhibitory assay revealed that Aspergillus Versicolor extracts had an inhibitory percentage of 86.67%. Furthermore, the anti-inflammatory activity using COX1, COX2, TNF, and IL6 was 77.32, 85.21 %, 59.83%, and 56.15%; respectively. Additionally, the anti-viral effect using reverse transcriptase enzyme showed high antiviral activity with 92.10 %. Conclusion: The current study confirmed that the Aspergillus versicolor crude extract and its active constituents showed strong effects on diminishing the oxidative stress, neurodegenerative damage, antiinflammatory, anti-cancer and anti-viral, suggesting their beneficial role as a promising fermented product in the treatment of cancer, oxidative stress, Alzheimer's, anti-inflammatory and anti-viral diseases.

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