scholarly journals Standardization of Immunoassays for Measurement of High-Sensitivity C-reactive Protein. Phase I: Evaluation of Secondary Reference Materials

2003 ◽  
Vol 49 (4) ◽  
pp. 611-616 ◽  
Author(s):  
Mary M Kimberly ◽  
Hubert W Vesper ◽  
Samuel P Caudill ◽  
Gerald R Cooper ◽  
Nader Rifai ◽  
...  
Keyword(s):  
Reference Material ◽  
Reference Materials ◽  
High Sensitivity ◽  
Acute Phase Reactant ◽  
Population Based ◽  
Medical Decision ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Decision Points ◽  
Fresh Serum

Abstract Background: Inflammation contributes to the development and progression of atherosclerosis, and C-reactive protein (CRP) can be used as a marker to assess risk for cardiovascular diseases. As variability among existing high-sensitivity CRP (hsCRP) assays can lead to misclassification of patients and hamper implementation of population-based medical decision points, standardization of hsCRP assays is needed. Methods: We evaluated five proposed secondary reference materials, including two diluted preparations of Certified Reference Material 470 (CRM470), two preparations of a serum-based material with recombinant CRP added, and one serum-based material with isolated CRP added. Twenty-one manufacturers participated in the comparison with 28 different assays. We examined imprecision, linearity, and parallelism with these materials and with fresh serum. Results: All materials had similar imprecision; CVs for the undiluted materials were 2.1–3.7%. None of the materials was linear across all assays. Each had between one and three cases of nonlinearity, with one preparation of CRM470 having the fewest cases of nonlinearity. Although none of the materials was parallel across all assays, the differences in slope from fresh serum were similar across all assays. Conclusions: All materials performed similarly with regard to imprecision, linearity, and parallelism. As one preparation of CRM470 had slightly better characteristics than the other materials and because CRM470 had been certified previously as a reference material for the acute-phase reactant range, it will be used in the next phase to standardize hsCRP assays.

scholarly journals A population-based dietary inflammatory index predicts levels of C-reactive protein in the Seasonal Variation of Blood Cholesterol Study (SEASONS)

2013 ◽  
Vol 17 (8) ◽  
pp. 1825-1833 ◽  
Author(s):  
Nitin Shivappa ◽  
Susan E Steck ◽  
Thomas G Hurley ◽  
James R Hussey ◽  
Yunsheng Ma ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
High Sensitivity ◽  
Population Based ◽  
Blood Cholesterol ◽  
Dietary Inflammatory Index ◽  
C Reactive Protein ◽  
Dietary Recall ◽  
Inflammatory Index ◽  
Reactive Protein ◽  
Hs Crp

AbstractObjectiveTo perform construct validation of the population-based Dietary Inflammatory Index (DII) using dietary data from two different dietary assessments and serum high-sensitivity C-reactive protein (hs-CRP) as the construct validator.DesignUsing data derived from (i) three 24 h dietary recalls (24HR) at baseline and at the end of each subsequent quarter (i.e. up to fifteen over a year) and (ii) a 7 d dietary recall (7DDR) measured at baseline and then quarterly, regression analyses were conducted to test the effect of the DII score on serum hs-CRP as dichotomous (≤3 mg/l, >3 mg/l), while controlling for important potential confounders.SettingExisting data from the Seasonal Variation of Blood Cholesterol Study (SEASONS), a longitudinal observational study of healthy participants recruited in Worcester, MA, USA and participants were followed for 1 year.SubjectsParticipants who had at least one hs-CRP measurement over her/his 1-year participation (n495 for 24HR,n559 for 7DDR).ResultsHigher DII scores were associated with values of hs-CRP >3 mg/l (OR = 1·08; 95 % CI 1·01, 1·16,P= 0·035 for the 24HR; and OR = 1·10; 95 % CI 1·02, 1·19,P= 0·015 for the 7DDR).ConclusionsThe population-based DII was associated with interval changes in hs-CRP using both the 24HR and 7DDR. The success of this first-of-a-kind attempt at relating individuals’ intakes of inflammation-modulating foods using this refined DII, and the finding that there is virtually no drop-off in predictive capability using a structured questionnaire in comparison to the 24HR standard, sets the stage for use of the DII in a wide variety of other epidemiological and clinical studies.

scholarly journals Association of C-Reactive Protein, Interleukin-1 Receptor Antagonist and Adiponectin with the Metabolic Syndrome

2007 ◽  
Vol 2007 ◽  
pp. 1-8 ◽  
Author(s):  
Juha Saltevo ◽  
Mauno Vanhala ◽  
Hannu Kautiainen ◽  
Esko Kumpusalo ◽  
Markku Laakso
Keyword(s):  
Metabolic Syndrome ◽  
Receptor Antagonist ◽  
Interleukin 1 ◽  
High Sensitivity ◽  
Population Based ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Interleukin 1 Receptor Antagonist ◽  
The Metabolic Syndrome ◽  
Hs Crp

This Finnish population-based study, mean age 46 years, evaluates the association of high-sensitivity C-reactive protein (hs-CRP), interleukin-1 receptor antagonist (IL-1Ra), and adiponectin with the NCEP and IDF definitions of metabolic syndrome (MetS). Adiponectin levels were higher, hs-CRP and IL-1Ra levels lower in subjects without MetS compared to subjects with MetS. If MetS was present according to both IDF and NCEP criteria, BMI, waist, triglycerides, hs-CRP, and IL-1Ra were significantly higher compared to subjects who had MetS according to either only IDF or only NCEP criteria. The hs-CRP, IL-1Ra, and adiponectin linearly correlated with the number of the components of MetS according to both definitions. Decreased levels of adiponectin and increased levels of hs-CRP and IL-1Ra are tightly associated with the components of MetS. Individuals who had MetS according to both criteria had the most adverse changes in cardiovascular risk factors.

Relation between glucose levels, high-sensitivity C-reactive protein (hs-CRP), body mass index (BMI) and serum and dietary retinol in elderly in population-based study

2012 ◽  
Vol 54 (3) ◽  
pp. 462-468 ◽  
Author(s):  
Rafaella C.P. Luna ◽  
Christiane C.C. do Nascimento ◽  
Luiza S.R. Asciutti ◽  
Sylvia do C.C. Franceschini ◽  
Rosália Gouveia Filizola ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Body Mass ◽  
High Sensitivity ◽  
Population Based ◽  
C Reactive Protein ◽  
Population Based Study ◽  
Glucose Levels ◽  
Reactive Protein ◽  
Hs Crp

scholarly journals Generalized anxiety and C-reactive protein levels: a prospective, longitudinal analysis

2012 ◽  
Vol 42 (12) ◽  
pp. 2641-2650 ◽  
Author(s):  
W. E. Copeland ◽  
L. Shanahan ◽  
C. Worthman ◽  
A. Angold ◽  
E. J. Costello
Keyword(s):  
Medication Use ◽  
High Sensitivity ◽  
Population Based ◽  
Generalized Anxiety ◽  
C Reactive Protein ◽  
Structured Interviews ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Health Related ◽  
Prospective Longitudinal

BackgroundGeneralized anxiety disorder (GAD) is highly co-morbid with depression. Depression is associated with elevated levels of the inflammation marker C-reactive protein (CRP), cross-sectionally and over time. To date, no studies have looked at the association between CRP and GAD.MethodA total of nine waves of data from the prospective population-based Great Smoky Mountains Study (n=1420) were used, covering children in the community aged 9–16, 19 and 21 years old. Structured interviews were used at each assessment to assess GAD symptoms, diagnosis and cumulative episodes. Blood spots were collected and assayed for high-sensitivity CRP levels.ResultsGAD was associated with increased levels of CRP in bivariate cross-sectional analyses. These bivariate associations, however, were attenuated after accounting for demographic, substance-use and health-related covariates. In longitudinal models, there was little evidence that CRP predicted later GAD. Associations from GAD to later CRP were attenuated in models adjusted for health-related coavariates and there was evidence that the GAD–CRP association was mediated by body mass index (BMI) and medication use.ConclusionsSimilar to depression, GAD was associated with elevated levels of CRP, but the effect of GAD on CRP levels was explained by the effect of GAD on health-related behaviors such as BMI and medication use. This study suggests differences in the association between inflammation and depression and GAD.

scholarly journals Higher Levels of Endogenous Estradiol are Associated with Frailty in Postmenopausal Women from the Toledo Study for Healthy Aging

2012 ◽  
Vol 97 (8) ◽  
pp. 2898-2906 ◽  
Author(s):  
L. Carcaillon ◽  
F. J. García-García ◽  
J. A. F. Tresguerres ◽  
G. Gutiérrez Avila ◽  
R. Kireev ◽  
...  
Keyword(s):  
Postmenopausal Women ◽  
Daily Living ◽  
Healthy Aging ◽  
High Sensitivity ◽  
Population Based ◽  
P Value ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Low Levels ◽  
Hs Crp

Abstract Background: Adverse effects of higher endogenous estradiol (E2) levels on various clinical outcomes and on determinants of the frailty syndrome have recently been reported. However, there are no data about the potential relationship between E2 and frailty. We aimed to study the association between E2 levels and frailty among older postmenopausal women not taking hormonal therapy. Methods: We used data from the Toledo Study for Healthy Aging, a Spanish population-based cohort study. Frailty was defined according to Fried's approach. Multivariate odds ratios (OR) and 95% confidence intervals (CI) associated with E2 levels were estimated using polytomous logistic regression. Results: E2 levels decreased significantly with age and educational level, whereas they increased with body mass index, high-sensitivity C-reactive protein (hs-CRP), and impairment in Katz activities of daily living. Higher E2 levels were associated with the prevalence of frailty among women younger than 79 yr, but not in the oldest group (p interaction = 0.047). After adjustment, OR of frailty associated with a 1 sd increase of E2 was 1.51 (95% CI, 1.04–2.20; P = 0.03). We identified an interaction between E2 and hs-CRP on the prevalence of frailty (P value = 0.042). Women with both higher E2 and hs-CRP (defined as values into the upper tertile) had an age-adjusted OR of 4.2 (95% CI, 1.7–10.5; P = 0.002), compared with women with low levels of both E2 and hs-CRP. Conclusion: Higher E2 levels were associated with frailty in postmenopausal women. The synergism between higher E2 and hs-CRP levels suggests the existence of physiopathological mechanisms connecting inflammation and estrogen to frailty.

scholarly journals C-Reactive Protein: Clinical and Epidemiological Perspectives

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Juan Salazar ◽  
María Sofía Martínez ◽  
Mervin Chávez ◽  
Alexandra Toledo ◽  
Roberto Añez ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Cardiovascular Risk ◽  
High Sensitivity ◽  
Acute Phase Reactant ◽  
Clinical Findings ◽  
Risk Scores ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Clinical Pictures ◽  
Score Systems

An important etiopathogenic component of cardiovascular disease is atherosclerosis, with inflammation being an essential event in the pathophysiology of all clinical pictures it comprises. In recent years, several molecules implicated in this process have been studied in order to assess cardiovascular risk in both primary and secondary prevention. C-reactive protein is a plasmatic protein of the pentraxin family and an acute phase reactant, very useful as a general inflammation marker. Currently, it is one of the most profoundly researched molecules in the cardiovascular field, yet its clinical applicability regarding cardiovascular risk remains an object of discussion, considered by some as a simple marker and by others as a true risk factor. In this sense, numerous studies propose its utilization as a predictor of cardiovascular risk through the use of high-sensitivity quantification methods for the detection of values <1 mg/L, following strict international guidelines. Increasing interest in these clinical findings has led to the creation of modified score systems including C-reactive protein concentrations, in order to enhance risk scores commonly used in clinical practice and offer improved care to patients with cardiovascular disease, which remains the first cause of mortality at the worldwide, national, and regional scenarios.

scholarly journals Study of serum high sensitivity C- reactive protein and lipid profile in ischemic and hemorrhagic stroke

2019 ◽  
pp. 42-45
Author(s):  
Dr. Sarika Argade ◽  
Dr. Reshakiran J Shendye
Keyword(s):  
Ischemic Stroke ◽  
Lipid Profile ◽  
Hdl Cholesterol ◽  
High Sensitivity ◽  
Haemorrhagic Stroke ◽  
Acute Phase Reactant ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cholesterol Levels ◽  
Hs Crp

Introduction: Stroke is the neurological deficit of abrupt onset attributable to focal vascular cause and makes a considerable contribution to morbidity and mortality. High sensitivity C reactive protein (hsCRP) is an acute-phase reactant tends to increase at the onset of inflammation. Atherosclerosis, a major risk factor for cerebrovascular diseases involves inflammation which is triggered by dyslipidaemia. Objective: To estimate and compare levels of serum hs-CRP and lipid profile in patients with ischemic and haemorrhagic stroke. Methods: Present study comprised of 90 subjects, 30 ischemic stroke, 30 haemorrhagic stroke and 30 as apparently healthy control. Blood samples obtained within 24 hours of presentation were analysed for serum hsCRP and lipid profile. Results: In the present study Median age was 52 years, 52.5 years and 54 years in control, ischemic stroke and haemorrhagic stroke respectively. Hs-CRP levels were raised in ischemic and haemorrhagic stroke compared to normal control (F-value=96.78; p<0.0001). Total cholesterol, triglyceride and LDL- cholesterol levels were significantly raised while HDL- cholesterol levels were low in ischemic stroke and haemorrhagic stroke than control (p<0.05). Conclusion: Increased serum hs-CRP levels and dyslipidemia were observed in ischemic and haemorrhagic stroke. But serum hs-CRP cannot differentiate type of stroke. Keywords: Ischemic stroke; Haemorrhagic stroke; hs-CRP; Lipid profile.

scholarly journals High sensitivity C-reactive protein level in cerebrovascular accident

2020 ◽  
Vol 7 (4) ◽  
pp. 666 ◽  
Author(s):  
Sonal Rajesh Kumar ◽  
T. A. Vidya
Keyword(s):  
Case Control Study ◽  
High Sensitivity ◽  
Acute Phase Reactant ◽  
Modified Rankin Scale ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cholesterol Levels ◽  
Significant Difference ◽  
Hs Crp ◽  
Control Study

Background: Stroke is the second leading cause of death worldwide according to WHO. High sensitivity C-Reactive Protein (hs-CRP) is an acute phase reactant which is being studied extensively to delineate its role in development of stroke as well as in prognostication.  This study was done to assess correlation of hs-CRP with risk factors of stroke and its association with types of stroke and prognosis.Methods: A prospective case control study of 100 patients with acute stroke along with 100 controls was conducted with informed consent.  At baseline, hs-CRP levels were measured and Modified Rankin Scale (MRS) was assessed. On day 90 the Modified Rankin Scale was assessed again. Patients were divided into groups based on hs-CRP levels and MRS and the results were analysed.Results: Prevalence of stroke was more in men than women (p=0.0002).  Statistically significant difference was found between mean hs-CRP levels in men (4.722±0.8982 mg/L) and women (4.133±0.9446 mg/L) (p=0.005) and between cases and controls (p=0.0003). There was no significant association with type of stroke (p=0.456).  Mean total cholesterol levels between cases and controls showed statistically significant difference (p=0.0005). High MRS was significantly associated with high hs-CRP levels (p=0.003).  Higher hs-CRP on day 1 correlated with higher MRS on day 90.Conclusions: hs-CRP level is increased in stroke and shows significant association with severity of stroke and prognosis.

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