scholarly journals PRELIMINARY STUDY ON THE DISTRIBUTION OF THE AEROBIC HETEROTROPHIC BACTERIA AND THE MICROBIAL INDICATORS IN JAKARTA BAY

2018 ◽  
Vol 20 ◽  
pp. 87-97
Author(s):  
Soeminarti S. Thayib ◽  
Flor Soehadi
Keyword(s):  
Water Samples ◽  
Human Activities ◽  
Vibrio Parahaemolyticus ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Microbial Indicators ◽  
Northern Side ◽  
Preliminary Study

The distribution of heterotrophic bacteria and microbial indicators in the Jakarta Bay were studied. Higher frequency of occurrences of the total coliform were observed in water samples collected near rivermouths or in the vicinity of islands. Lower frequency of occurrences of total coliform were obtained from samples collected from the far northern side of the Bay. The isolated strains belong to the genera Achromobacter, Vibrio, Flavobacteria, Micrococcus and coliforms. Micrococcus and coliforms were mostly obtained from samples collected at stations which are suspected to be highly influenced by human activities. The presence of Vibrio parahaemolyticus-like organisms were also determined. Occurrences of the genera varied unpredictably but were mostly within the six genera mentioned.

Microbiological water and effluent sample preservation

1980 ◽  
Vol 26 (8) ◽  
pp. 921-929 ◽  
Author(s):  
B. J. Dutka ◽  
A. El-Shaarawi
Keyword(s):  
Water Samples ◽  
Storage Temperature ◽  
Heterotrophic Bacteria ◽  
Stream Water ◽  
Bacterial Load ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Microbiological Analyses ◽  
Crushed Ice ◽  
Original Water

A study, using four types of water samples ranging from relatively pure stream water to water containing industrial and domestic effluents, was carried out to investigate the effect of storage temperature on four bacterial parameters: total coliform, fecal coliform, fecal streptococcus, and heterotrophic bacteria. In the study, each water sample was cooled immediately after collection to approximately 1.5 °C by storage in crushed ice. At 2-, 24-, 30-, and 48-h intervals, the sample was mixed, and a subsample was removed and tested. Three separate analyses for each parameter were made on each subsample. The data available for statistical analyses contained, in some cases, the values obtained for the three subsamples and, in others, the means of the three values. The data were analysed as replicated data and as part of the entire set. The analysed data indicated (i) that with the exception of heterotrophic populations, more than 75% of the samples were microbiologically stable for at least 24 h, (ii) that at least 50% of samples tested for heterotrophic densities were stable for a minimum period of 24 h, (iii) that the original water temperature and bacterial load do not appear to be consistent factors in the preservation of samples for microbiological analysis, and (iv) that nutrient levels, also, do not seem to be consistent factors in the preservation of water samples for microbiological analyses.

scholarly journals Analysis of the Physicochemical Parameters and Microbiological Quality of Water Samples Obtained from Mbiaso River and Enang Stream in Ikot Ekpene Metropolis

2021 ◽  
pp. 18-27
Author(s):  
Ekanem, Jonathan Okokon ◽  
Akpan, Nyaknoabasi Ime ◽  
Zorbari, Glory Msubaritoma
Keyword(s):  
Water Samples ◽  
Physicochemical Parameters ◽  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Titratable Acidity ◽  
Total Coliform ◽  
Coliform Count ◽  
Quality Of Water ◽  
Sampling Points ◽  
Who Standards

The physicochemical, microbiological and heavy metal studies of water samples obtained from Mbiaso River and Enang Stream were investigated. Water samples were collected from two locations with two sampling points from each location along the course of the water body based on their use by the community and analysed using standard procedures. The results obtained were compared with WHO standards for drinking and recreational water. Aside titratable acidity, titratable alkalinity and BOD, all other physicochemical parameters were within the permissible standards. All the heavy metals concentrations were above the permissible limits except for Lead (Pb) that ranged between 0.01mg/l to 0.05 mg/l for all the sampling points. All the water samples were found to harbor coliforms organisms in numbers greater than the required WHO standards for water except for Enang river that lacked the presence of Vibrio spp. The total viable counts for all the water samples were generally high exceeding the standard limit of 1.0 x 102 cfu/ml for water. The total heterotrophic bacteria count, total coliform count, faecal coliform count and total fungal count ranged between 1.9x104 - 52x104 cfu/ml, 2.3x104 -38 x104 cfu/ml, 1.7x104 - 30x104 cfu/ml and 2.5x104 - 5.3x104 cfu/ml respectively. The total salmonella-shigella counts and total staphylococcus counts were in the range of 0.2 x102-0.4 x102 cfu/ml and 0.3 x102 -0.6 x104 cfu/ml respectively. From the study, it is suggested that some regulations measures be put in place to control pollution of the water while the water be properly treated before use for domestic, potable or recreational purposes.

scholarly journals Assessment of Physicochemical and Microbiological Characteristics of Water Samples of Okomu National Park, Edo State, Nigeria

2020 ◽  
pp. 7-17
Author(s):  
A. O. Omonona ◽  
A. T. Adetuga ◽  
T. V. Solotan
Keyword(s):  
Water Samples ◽  
National Park ◽  
Heterotrophic Bacteria ◽  
Oxygen Demand ◽  
Total Coliform ◽  
Permissible Limit ◽  
Grab Sampling ◽  
Microbiological Characteristics ◽  
Significant Difference ◽  
Edo State

Water is very crucial for the survival and maintenance of most living organisms. The quality of water available for plant, animal or human use cannot therefore be compromised. This study therefore examined the physicochemical and microbiological characteristics of water samples in Okomu National Park, Edo State, Nigeria. Water samples were collected from four waterholes (three rivers and one stream) for two seasons (dry and wet) using grab sampling technique. These water samples were analysed for physicochemical [temperature, pH, total dissolved solid, electrical conductivity, total suspended solids, dissolved oxygen (DO), nitrate, chloride, phosphate, sulphate, biological oxygen demand (BOD) and chemical oxygen demand (COD)] and microbiological (total coliform and fungal counts) analyses using standard methods. Data collected were subjected to descriptive statistics and T-test at α0.05 and compared with WHO permissible limits. The result showed that the mean COD (both seasons) and BOD (dry season) were above the comparable WHO permissible limit while there was no significant difference in the values of all the physicochemical parameters across the seasons of sampling. Salmonella / Shigella and Staphylococcus aureus were observed to be absent while the total coliform and fungi counts were observed to be higher than the WHO permissible limit for drinking water. The detection of Escherichia coli in the water samples (except Arakhuan stream) is an indication of faecal contamination either of animal or human origin. There was significant difference in total heterotrophic bacteria (t=-4.936) and total coliform counts (t=-2.417) in the waters sampled. Constant monitoring of the waterholes and intensified sensitization of the surrounding communities on ecosystem conservation is needed to protect the park’s ecosystems.

scholarly journals Investigations on Bacteriological Quality of Tap Water Sources within the University of Port Harcourt

2019 ◽  
pp. 1-5
Author(s):  
H. O. Stanley ◽  
C. J. Ugboma ◽  
P. C. Uzoaru
Keyword(s):  
Water Samples ◽  
Membrane Filtration ◽  
Heterotrophic Bacteria ◽  
Tap Water ◽  
Total Coliform ◽  
Water Sources ◽  
Filtration Method ◽  
Port Harcourt ◽  
The University

In this study, the sanitary quality of tap water sources within the University of Port Harcourt was investigated in order to determine its suitability for drinking and other domestic applications. Eight composite samples of tap water were collected from Eight different locations within the three campuses of the University of Port Harcourt using 300 ml-capacity sterile containers. These locations included NDDC Hostel, Sports Hostel, Medical Hostel, NUH Hostel, Dan Etete Hostel, Delta A Hostel, Delta B Hostel and Choba Campus. After collection, water samples were taken to the laboratory for enumeration and identification of Total heterotrophic bacteria (THB), Total coliform (TC) and Faecal coliform (FC) using the membrane filtration method. Result showed that the average THB, TC and FC counts recorded in the tap water samples across the eight locations ranged from 12.4 CFU to 36.7 CFU, 4.3 CFU to 10.1 CFU and 2.8 CFU to 5.2 CFU per 100ml respectively. Bacterial isolates were identified as probably belonging to genera such as Bacillus spp., Klebsiella spp., Enterobacter spp., Staphylococcus spp., Proteus spp., Citrobacter spp., Serratia spp. and Escherichia spp., respectively. Samples from Delta B hostel and Choba campus recorded the lowest and highest THB, TC and FC counts respectively. According to WHO standard, the result of THB, TC and FC counts indicated that the tap water in all eight locations were above the limit acceptable for drinking but within acceptable limit for other domestic use such as bathing and washing.

scholarly journals Evaluation of the Bacteriological and Physicochemical Qualities of Some Brands of Bottled Water Sold in Owerri, Imo State

2021 ◽  
pp. 8-17
Author(s):  
Kingsley Kelechi Onyekachi ◽  
Joy Nkeiruka Dike-Ndudim ◽  
Emeka Simon Anikwo ◽  
Chizaram Winners Ndubueze
Keyword(s):  
Water Samples ◽  
Fecal Coliform ◽  
Membrane Filtration ◽  
Heterotrophic Bacteria ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Total Coliform ◽  
Bottled Water ◽  
Bacterial Agent ◽  
Filtration Technique

This work was carried out in order to detect the presence of bacterial agent in the bottled water samples, and to evaluate the physicochemical qualities of these bottled water. Twenty samples selected from four different brands of bottled water sold in Owerri, were examined to determine their bacteriological and physicochemical qualities. Bacteriological analyses using Membrane filtration technique was carried out to determine the heterotrophic bacteria, total coliform and fecal coliform counts. Physicochemical qualities were also determined using standard methods. The heterotrophic bacterial count ranged from 0.00 to 12.00 CFU/ml, total coliform count ranged from 0.00 to 7.50 coliform/100ml. There was absence of fecal coliform in the samples. The pH, Color, Turbidity and Conductivity of the samples ranged from 5.18 to 7.28, 0.00 to 18.00 PCU, 0.55 to 1.62 NTU and 16.00 to 262.00 µs/cm respectively.  The Chloride, Iron and Nitrite content of the samples ranged from 16.99 to 27.98 mg/L, 0.01 to 0.07 mg/L Fe, and 0.00 to 0.34 mg/L respectively. The bacterial species isolated belong to the genera Staphylococcus, Proteus, Klebsiella, Bacillus and Escherichia. Quality wise, most of the water samples met the XYZ standard for bacteriological and physicochemical quality for drinking water with the exception of few. It can be deduced from this study, that none of the bottled water is suitable for drinking with regards to the bacteriological and physicochemical parameters tested. Though some samples passed the bacteriological examination, most of them were acidic and therefore not safe for consumption.

scholarly journals WATER QUALITY INDICATOR BACTERIA IN BENGAWAN SOLO AND PORONG RIVERS AND THEIR ESTUARIES

2018 ◽  
Vol 27 ◽  
pp. 1-17
Author(s):  
Soeminarti S. Thayib ◽  
Ruyitno ◽  
Djoko Hadi Kunarso ◽  
Hamidah Razak
Keyword(s):  
Water Samples ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Bacterial Population ◽  
Total Coliform ◽  
Indicator Bacteria ◽  
Water Quality Indicator ◽  
Freshwater Bacteria ◽  
River Mouths

Water samples taken from rivers and estuaries stations in Bengawan Solo and Porong rivers in East Java were analyzed for the pollution of indicator bacteria in July-August and NovemberDecember 1984, to determine the influences of domestic wastes on the quality of these waters. Higher frequency of occurrences of high total coliform, fecal coli and fecal streptococci were observed in water samples collected in river stations, river mouths and in the vicinity of dense populated areas. The distribution of saprophytes, freshwater bacteria and indicator bacteria were also studied. The changes in the bacterial population as funciton of the salinity were shown during the observations. Some pathogenic bacteria like Salmonella, Yarsinia, and Vibrio parahaemolyticus and others have been isolated from waste and mud samples.

scholarly journals Impact of Bisphenol A on the Physicochemical and Bacteriological Characteristics of Water in Storage Tanks from various Locations in Salem University Lokoja, Kogi State, Nigeria

2020 ◽  
Vol 24 (2) ◽  
pp. 223-229
Author(s):  
U. Udochukwu ◽  
P.G. Olannye
Keyword(s):  
Bisphenol A ◽  
Water Samples ◽  
Heterotrophic Bacteria ◽  
Methyl Chloride ◽  
Total Coliform ◽  
Storage Tanks ◽  
University Environment ◽  
Borehole Water ◽  
The University ◽  
The Impact

This study examined the impact of Bisphenol A (BPA) on the physicochemical and bacteriological characteristics of water in storage tanks in Salem University Lokoja. Borehole water samples were collected from three (3) locations within the University environment and stored in a jerry can for analysis. Total Heterotrophic Bacteria Count (THBC) in water samples ranged from 1.00± 0.30 x104 at week 0 to 8.95±1.00 x104 cfu/ml at week 3, while the total coliform count (TCC) also ranged from 1.30±0.15 x104 to 7.11±0.82 x104 cfu/ml. TCC and THBC were found to be higher than the NSDWQ Standard. The identified isolates from the borehole samples were Escherichia coli, Pseudomonas aeruginosa, Streptococcus faecalis, Bacillus cereus, Staphylococcus epidermidis and Serretia spp. Bisphenol A (BPA) was not detected in week 0, after week 3, components of Bisphenol A detected were methyl chloride, Benzene and Dichlorobenzene and their highest values were 0.054±0.033, 0.021±0.020 and 0.055±0.062 mg/l respectively. The pH, Turbidity, Total suspended solids, BOD and conductivity reduced as storage increased. Magnesium and calcium for sample B were found to have the highest value of 0.31 and 1.73 mg/l respectively. Storage of water for a long period of time should be discouraged as it could trigger increased leaching of BPA into the water which will affect its physicochemical and microbiologically quality. Keywords: Bacteria, Bisphenol A, Storage Water Tanks, Drinking Water Quality.

MICROBES PARAMETERS OF WATER QUALITY FOR AQUACULTURE ON PARI ISLAND WATERS

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Lies Indah Sutiknowati
Keyword(s):  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Total Cell ◽  
Epifluorescence Microscopy ◽  
Coliform Bacteria ◽  
Total Density ◽  
Shellfish Aquaculture ◽  
Microbiological Parameters ◽  
Phosphate Nitrate

The objective of this research was to evaluate waters quality in Pari island waters for aquaculture purpose based on bacteriological information conducted in Mei and September 2011. Microbiological parameters analyzed were total density of bacteria for coliforms, E.coli, pathogenic, heterotrophic, halotoleran, phosphate-nitrate-ammonia breaker, and total cells. Method to analyze coliform bacteria was filtration, identification of pathogenic bacteria using biochemical test, density analises for heterotrophic bacteria, analises for phosphate-nitrate-ammonia breaker bacteria using pour plate, and total cell using Acridine Orange Epifluorescence Microscopy. Results showed that the abundance of total coliform cell was about 1000-7000 colony forming unit (cfu)/100 ml. The abundance of heterotrophic, halotolerant, phosphate-nitrate-ammonia bacteria in seawater was (3.6-4.3)x105 cfu/ml, (1.1-1.3)x105 cfu/ml, (0.5-3.44)x103cfu/ml; and (3.6-6.7)x105 cfu/ml, (1.6-2.7)x105 cfu/ml, (0.6-5.22)x103 cfu/ml in sediment, respectively. The total cell of bacteria was (0.05-2.1)x107cells/ml. The dog-conch (Strombus turturella) and blood-clamps (Anadara granosa) can survive in Pari Island and there was a significant increase in sea grass litter with growth average of 0.67 mm/day and 0.90 mm/day. During snails and clamps growth, there were found several genus of pathogenic bacteria such as Salmonella, Vibrio, Aeromonas, Pseudomonas, Citrobacter, Proteus, Shigella, Hafnia, and Yersinia. The results showed that Pari island waters was suitable for developing shellfish aquaculture dog conch and blood clamps. Keywords: bacteria, parameter, shellfish, aquaculture.

Microbiological analysis of Udo Anwankwo river in Ikot Ekpene, South-South Nigeria

2021 ◽  
Vol 1 (2) ◽  
pp. 005-011
Author(s):  
Jonathan Okokon Ekanem ◽  
Divine Jacob Ottong
Keyword(s):  
Aspergillus Niger ◽  
Water Sample ◽  
Water Samples ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Proper Treatment ◽  
Fungal Isolates ◽  
Standard Procedures ◽  
Sampling Points ◽  
Microbial Analysis

The microbiological study of water samples obtained from Udo Anwankwo River was investigated. Water samples were collected from three different sampling points along the course of the river and analyzed using standard procedures. The total bacterial counts, total coliform counts and total fungal counts of the water samples ranged from 2.6×105 to 4.8×105cfu/ml, 1.2×104 to 1.8×105cfu/ml and 0.24×103 to 1.9×103cfu/ml respectively. A total of nine bacteria species belonging to the following genera, Bacillus, Salmonella, Escherichia, Pseudomonas, Micrococcus, Staphylococcus, Vibrio, Enterobacter and Streptococcus were isolated and identified from the samples, while five fungal isolates including members of the genera Aspergillus niger, Mucor, Penicillum, Rhizopus and Fusarium were isolated. The study through microbial analysis has revealed that the river water sample was not free from pathogens and thereby not suitable for potable use. There is need to put adequate measures towards the control of pollution and proper treatment of the water before usage as it contains pathogenic organisms.

scholarly journals Yeasts and filamentous fungi in bottled mineral water and tap water from municipal supplies

2007 ◽  
Vol 50 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mirian Ueda Yamaguchi ◽  
Rita de Cássia Pontello Rampazzo ◽  
Sueli Fumie Yamada-Ogatta ◽  
Celso Vataru Nakamura ◽  
Tânia Ueda-Nakamura ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Water Samples ◽  
Mineral Water ◽  
Tap Water ◽  
Health Hazard ◽  
Total Coliform ◽  
Species Level ◽  
Potential Health ◽  
Potential Health Hazard ◽  
Bottled Mineral Water

The main objective of this study was to analyse the occurrence of yeasts and filamentous fungi in drinking water as well as to investigate their correlation with the indicator bacteria of faecal pollution. Yeasts were detected in 36.6% and 11.6% of the bottled mineral on water dispensers and tap water samples from municipal system, respectively. Twenty-one (35.0%) of bottled mineral water and two (3.3%) of tap water samples were positive for filamentous fungi. For bottled mineral water 12 (20.0%) of 60 samples were positive for total coliform, compared with 3(5.0%)out of 60 samples from tap water. The mineral water from dispensers was more contaminated than tap water. Strains belonging to the genera Candida identified to the species level were C. parapsilosis, C. glabrata and C. albicans. Thus, bottled mineral water from water dispensers and tap water could be considered a possible transmission route for filamentous fungi and yeasts, and could constitute a potential health hazard, mainly to immunocompromised indivuals.

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