Comparison of serial blood lactate level between dengue shock syndrome and dengue hemorrhagic fever (evaluation of prognostic value)

Background Dengue shock syndrome (DSS) mortality is still high.Monitoring of blood lactate level is important to evaluate shock.Objectives The study were to review the difference between bloodlactate level of DSS and that of dengue hemorrhagic fever (DHF),to correlate blood lactate level with hypoxia state as shock riskfactors (PaO 2 , oxygen saturation, and anion gap) and to determinethe cut-off point of blood lactate level to predict shock.Methods The study was carried out at the Department of ChildHealth, Medical School, University of Indonesia, CiptoMangunkusumo Hospital, Jakarta, from January until July 2006.Three mL venous blood specimen was collected from all subjectsfor peripheral blood, blood gasses, serology, and blood lactateexaminations. This study consisted of a retrospective cohort anda cross sectional method. Data were analyzed with Chi-squaretest. Continous data tested using Mann-Whitney method. Toknow the correlation between blood lactate level and shock riskfactors we use logistic regression test.Results In DSS group, 73% shows hyperlactatemia (lactate =2mmol/L). Conversion of lactate means between two groups issignificantly different from day one to day two and three. Therewas a negative correlation between lactate level and pO 2 andoxygen saturation. Oxygen saturation is the only value that hasclinical correlation. Regressions analysis can be applied using Y= 7.05–0.05 X equation. The cut-off point of lactate level asmarker for shock by using ROC curve is 32.015 mmol/L with 70%sensitivity and 83.3% specificity.Conclusions Hyperlactatemia in DSS can be considered as a signfor unappropriate treatment of shock. Blood lactate level can beused as a biochemical marker for tissue hypoxia, to assess severityof the disease, as monitoring of treatment, and has prognosticvalue of DHF cases.

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Hyperventilation in Children with Dengue Hemorrhagic Fever (DHF)

Paediatrica Indonesiana ◽

10.14238/pi31.9-10.1991.245-56 ◽

2019 ◽

Vol 31 (9-10) ◽

pp. 245-56

Author(s):

Kasim Y. A. ◽

Anky Tri Rini K. E. ◽

Sumarmo S. P. S.

Keyword(s):

Dengue Hemorrhagic Fever ◽

Dengue Shock Syndrome ◽

Hemorrhagic Fever ◽

Lung Edema ◽

Respiratory Problems ◽

Ventilatory Pattern ◽

Grade Ii ◽

Plasma Leakage ◽

The Difference ◽

Respiratory Disturbance

Many studies of Dengue Hemorrhagic Fever (DHF) have been done but only a few revealed the respiratory status. Respiratory problems arise. because of plasma leakage through the 'damaged capillaries, causing lung edema and in turn result in hypoxemia. This later on will be compensated by a hyperventilation state. During a 6-month-period (May to September 1988), two aspects were studied in 85 patients hospitalized with DHF. First, the ventilatory pattern and second, the result of giving oxygen support in improving the respiratory disturbance, in this case alveolar hyperventilation. The incidence of alveolar hyperventilation in DHF grade II (DHF II) and Dengue Shock Syndrome (DSS) differed significantly. Hypoxemia occurred in DHF II and DSS with no significant differences. The difference of the incidence of metabolic acidosis in DHF II and DSS were significant. In DHF II patients having had hyperventilation state, oxygen therapy decreased respiration rate significantly and increased the PaC02 though not significantly.

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Awareness of Dengue hemorrhagic fever and Dengue shock syndrome: A cross-sectional survey

ISEE Conference Abstracts ◽

10.1289/isee.2020.virtual.p-0567 ◽

2020 ◽

Vol 2020 (1) ◽

Author(s):

W. Elodie ◽

F. Zhi

Keyword(s):

Dengue Hemorrhagic Fever ◽

Dengue Shock Syndrome ◽

Hemorrhagic Fever ◽

Cross Sectional Survey ◽

Cross Sectional

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Effect of dengue hemorrhagic fever on thrombomodulin level

Paediatrica Indonesiana ◽

10.14238/pi49.5.2009.259-63 ◽

2009 ◽

Vol 49 (5) ◽

pp. 259

Author(s):

Hendra Widjaja ◽

Max F. J. Mantik

Keyword(s):

Endothelial Cells ◽

Dengue Hemorrhagic Fever ◽

Pediatric Patients ◽

Hemorrhagic Fever ◽

Cross Sectional Study ◽

Blood Analysis ◽

Cross Sectional ◽

Significant Difference ◽

The Difference ◽

Difference Test

Background Thrombocyte and endothelial cells play animportant role in dengue hemorrhagic fever pathogenesis.Thrombomodulin is a part of glycoprotein membrane inendothelial cells. Therefore, thrombomodulin level willincrease if endothelial cells disruption occurs.Objective To acknowledge the correlation between thedegree of dengue hemorrhagic fever and thrombomodulinlevel.Methods This was a cross-sectional study. Subjects werehospitalized pediatric patients with age ranging from oneto 13 year old in pediatric ward at Pro£ Dr. R.D. KandouHospital, Manado, who had fever. Three milliliters of bloodwere taken from vein, and were divided for two tests whichwere routine blood analysis and thrombomodulin analysis.Different data resulted from the dengue hemorrhagic fevergroup were processed, and analyzed statistically using F Testand LSD (least significant difference) test. The relationbetween dengue hemorrhagic fever and thrombomodulinwas analyzed with Spearman correlation coefficient.Results There was a significant result in the difference ofthrombomodulin level on four dengue hemorrhagic fevergroups which were classified according to the severity ofdengue hemorrhagic fever. There was a very significantpositive correlation between the severity of denguehemorrhagic fever and thrombomodulin level in detectingendothelial cells impairment.Conclusion Thrombomodulin level can be used as amarker to detect endothelial cells impairment in denguehemorrhagic fever. Higher grade of dengue hemorrhagicfever will have higher thrombomodulin level.

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The Difference of sVE-Cadherin Levels between Dengue Hemorrhagic Fever Patients with Shock and without Shock

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.602 ◽

2019 ◽

Vol 7 (14) ◽

pp. 2277-2281 ◽

Cited By ~ 1

Author(s):

Rinang Mariko ◽

Eryati Darwin ◽

Yanwirasti Yanwirasti ◽

Sri Rezeki Hadinegoro

Keyword(s):

Dengue Virus ◽

Sample Size ◽

Dengue Hemorrhagic Fever ◽

Hypovolemic Shock ◽

Hemorrhagic Fever ◽

Total Sample ◽

Research Subjects ◽

Cross Sectional ◽

The Difference ◽

Cross Sectional Design

BACKGROUND: Dengue virus infection is an infectious disease caused by the dengue virus and transmitted by the Aedes aegypti mosquito. Dengue virus (DEN-V) consists of 4 serotypes, namely DEN-1, DEN-2, DEN-3, and DEN-4. The most feared result of DHF is death. Death in children is caused by hypovolemic shock due to plasma leakage from intravascular to extravascular space due to endothelial dysfunction. AIM: This study aims to analyse difference in sVE-Cadherin levels in Dengue Hemorrhagic Fever (DHF) with and without shock. MATERIAL AND METHODS: The method of taking samples is consecutive sampling, namely the research subjects obtained based on the order of entry in the hospital with a comparative cross-sectional design. From the results of the calculation using the sample formula, the sample size for each group is set at 32 people. So that the total sample size used for both groups is 64 people. The serum sVE-Cadherin levels using the ELISA method. The statistical test used is the independent t-test. The value of p < 0.05 was said to be statistically significant. RESULTS: The result showed that there was no difference in mean sVE-Cadherin levels between DHF patients with shock and without shock (p > 0.05). CONCLUSION: This study concluded that there was no difference in mean of sVE-Cadherin level in DHF patients with shock and without shock.

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Pengaruh ekspresi p53 dan HIF1 terhadap peningkatan laktat jaringan nasofaring pada pasien karsinoma nasofaring

Oto Rhino Laryngologica Indonesiana ◽

10.32637/orli.v48i1.258 ◽

2018 ◽

Vol 48 (1) ◽

pp. 74

Author(s):

Jemmy Kurniawan ◽

Pudji Rahaju ◽

Soehartono Soehartono

Keyword(s):

Blood Lactate ◽

Lactate Level ◽

Elevated Level ◽

Hypoxia Inducible Factor ◽

Blood Lactate Level ◽

Cross Sectional ◽

Hypoxia Inducible Factor 1 ◽

Nasopharyngeal Tissue ◽

Lactate Levels ◽

The Impact

Latar Belakang: Karsinoma nasofaring (KNF) merupakan keganasan tersering pada kepala dan leher. Pilihan terapi KNF adalah radioterapi dan kemoterapi yang berhubungan dengan toksisitas, resistensi obat, dan rekurensi. Intervensi metabolik yang didasarkan pada perubahan metabolisme sel kanker merupakan salah satu strategi terapi kanker pada saat ini. Untuk dapat mengetahuinya perlu dipahami pengaruh ekspresi p53 dan hypoxia-inducible factor 1 (HIF1) terhadap peningkatan kadar laktat jaringan nasofaring pada pasien KNF. Tujuan: Mengetahui pengaruh ekspresi p53 dan HIF1 terhadap peningkatan kadar laktat jaringan nasofaring, dan untuk mengetahui kesesuaian antara kadar laktat darah dengan laktat jaringan nasofaring. Metode: Penelitian cross sectional melibatkan 10 subjek, dilakukan biopsi nasofaring dengan tuntunan nasoendoskopi untuk pemeriksaan histopatologi, ekspresi p53 dan HIF1 dengan imunohistokimia, laktat jaringan nasofaring dengan colorimetric, dan laktat darah. Hasil: Seluruh subjek mengalami peningkatan ekspresi p53 dan HIF1 dengan rerata p53 19,53±7,37 dan HIF1 24,30±12,28. Seluruh subjek penelitian memiliki kadar laktat jaringan meningkat, dengan rerata kadar laktat 0,67±0,39. Kadar laktat darah subjek cenderung meningkat dengan rerata 2,93±0,65. Terdapat pengaruh peningkatan ekspresi p53 terhadap peningkatan kadar laktat jaringan (p=0,002). Terdapat pengaruh peningkatan ekspresi HIF1 terhadap peningkatan kadar laktat jaringan (p=0,042). Tidak terdapat kesesuaian antara kadar laktat darah dengan laktat jaringan nasofaring (p=0,000). Kesimpulan: Peningkatan ekspresi p53 dan HIF1 berpengaruh terhadap peningkatan kadar laktat jaringan nasofaring pada pasien KNF, namun kadar laktat darah tidak menggambarkan kadar laktat jaringan nasofaring. ABSTRACTBackground: Nasopharyngeal carcinoma (NPC) is the most frequent malignancy of the head and neck. The options of NPC therapy are radiotherapy and chemotherapy, associated with toxicity, drug resistance, and recurrence. Metabolic intervention based on changes in cancer cell metabolism is currently one of the strategies of cancer therapy. Aim: To determine the impact of p53 and hypoxia-inducible factor 1 (HIF1) expression on elevated lactate levels of nasopharyngeal tissue, and to determine the compatibility between blood lactate and nasopharyngeal tissue lactate levels in patients with NPC. Method: This cross-sectional study involved 10 subjects who underwent nasopharyngeal biopsy for histopathologic examination, p53 and HIF1 expression using immunohistochemistry, lactate of nasopharyngeal tissue using colorimetric, and blood lactate. Results: All subjects had increased expression of p53 and HIF1 with p53 mean of 19.53±7.37 and HIF1 mean of 24.30±12.28. All subjects had elevated tissue lactate levels, with lactate levels mean of 0.67±0.39. The blood lactate level of the subjects increased, with blood lactate level mean of 2.93±0.65. There was a significant increasing impact of p53 expression on tissue lactate elevated level (p=0.002) and a significant increasing impact of HIF1 expression on tissue lactate elevated level (p=0.042). There was no correlation between lactate levels of blood lactate and nasopharyngeal tissue (p=0.000). Conclusion: Increased expression of p53 and HIF1 had an effect on increased levels of lactate nasopharyngeal tissue in NPC patients, but blood lactate levels did not have a correlation with lactate levels of nasopharyngeal tissue.

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Correlation Analysis between Ratio of C-Reactive Protein/Albumin and Severity of Dengue Hemorrhagic Fever in Children

Indonesian Journal of Tropical and Infectious Disease ◽

10.20473/ijtid.v9i3.29108 ◽

2021 ◽

Vol 9 (3) ◽

pp. 136

Author(s):

Agustin Iskandar ◽

Yuyun Norwahyuni ◽

Aryati Aryati ◽

Andrea Aprilia

Keyword(s):

Dengue Hemorrhagic Fever ◽

Pearson Correlation ◽

Dengue Infection ◽

Dengue Shock Syndrome ◽

Hemorrhagic Fever ◽

Bromocresol Green ◽

C Reactive Protein ◽

Cross Sectional ◽

Albumin Ratio ◽

Reactive Protein

Dengue Hemorrhagic Fever (DHF) is a dengue infection which can cause shock and leads to mortality. Hypoalbuminemia is a marker of plasma leakage in DHF and correlated with severity of in fl ammatory response triggered by infection, including DHF. C-Reactive Protein (CRP) is a proin fl ammatory marker that also increases in DHF. This study aims to determine a correlation of CRP/albumin ratio with severity of DHF. Cross sectional study on pediatric patients diagnosed as DHF at Saiful Anwar Malang Hospital was done in July-December 2016. CRP levels were examined using immunoturbidimetry method, while albumin was examined by using Bromocresol Green (BCG) method. Correlation of CRP/albumin ratio with DHF severity was analyzed by using Pearson correlation test.The result showed that there were signi fi cant diff erences in CRP levels and CRP/albumin ratios in the Dengue Shock Syndrome (DSS) and non-DSS group (p = 0.002, p = 0.001, α<0.05). There was no signi fi cant diff erence in albumin level in the same group (p = 0.207, α <0.05). Positive correlation found in CRP and CRP/albumin ratio (r = 0.46, r = 0.49, α <0.01). On the contrary the negative correlation was found in albumin (r = -0.21, α <0.01). This is presumably because albumin is an acute phase protein which will decrease along with the severity of infection. In contrast, CRP will increase during the critical phase of infection. It can be concluded that the CRP/albumin ratio was positively correlated with DHF severity, as well as CRP levels, but not positively correlatedwith albumin.

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Study of Clinical and Laboratory Profile in Patients With Dengue Fever at A Tertiary Care Center in Central Nepal

Nepal Medical Journal ◽

10.37080/nmj.121 ◽

2020 ◽

Vol 3 (2) ◽

pp. 1-8

Author(s):

Nirmal Ghimire ◽

Damodar Paudel ◽

Manoj Kumar Yadav ◽

Suraj Rana ◽

Navin Kumar Chaudhary ◽

...

Keyword(s):

Dengue Fever ◽

Dengue Hemorrhagic Fever ◽

Viral Infections ◽

Wide Spectrum ◽

Care Center ◽

Tertiary Care ◽

Dengue Shock Syndrome ◽

Hemorrhagic Fever ◽

Viral Disease ◽

Cross Sectional

Introduction: Dengue is a mosquito-borne viral disease transmitted from person to person by Aedes mosquitoes which result in a wide spectrum of disease severity ranging from influenza-like illness (dengue fever; DF) to the life-threatening dengue hemorrhagic fever (DHF)/dengue shock syndrome (DSS). Terai regions of Nepal were focal epidemics of Dengue infections during the outbreak in 2010, 2013, and 2016. Dengue infections have been reported in the valleys of upland Hill regions at an altitude of 2500 m above sea level in Nepal. Methods: A cross-sectional study was carried out among febrile patients in Nepal Police Hospital (NPH), from 1st Baisakh 2076 to 30th Chaitra 2076. Blood samples were collected from dengue presumed cases and tested against dengue specific IgM antibody and/or NS1antigen. Clinical examination findings were recorded, hematological and biochemical parameters tests were done among the patients who fulfilled the inclusion criteria. Results: A total of 87 dengue cases were included in the study during the study period. Out of these, the majority were males ( 85.05%) from Kathmandu (38/87; 43.67%) seen in the month of Asoj (40/87; 45.98%). Fever was the major symptom (100%) followed by myalgia (52.87%), headache (45.97%), retro-orbital pain (12.64%), bleeding manifestations (9.19%). Common hematological abnormalities were thrombocytopenia and leucopenia in the critical phase. There was no case of dengue shock syndrome. Conclusion: This study highlights the utilization of most common clinical and easily available laboratory profiles of dengue viral infections in particular season and place that could alert physicians to diagnose early to reduce morbidity and mortality due to dengue hemorrhagic fever and dengue shock syndrome.

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Effect of transfusion on the venous blood lactate level in very low-birthweight infants

Pediatrics International ◽

10.1111/j.1442-200x.2008.02733.x ◽

2009 ◽

Vol 51 (3) ◽

pp. 321-325 ◽

Cited By ~ 8

Author(s):

Daijiro Takahashi ◽

Miyu Matsui ◽

Reiko Shigematsu ◽

Tetsuji Sato ◽

Ryosuke Miyaji ◽

...

Keyword(s):

Blood Lactate ◽

Lactate Level ◽

Low Birthweight ◽

Venous Blood ◽

Blood Lactate Level ◽

Very Low Birthweight ◽

Very Low Birthweight Infants ◽

Venous Blood Lactate

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Perbedaan Penggunaan Antikoagulan K2EDTA DAN K3EDTA Terhadap Hasil Pemeriksaan Indeks Eritrosit

Jurnal Laboratorium Khatulistiwa ◽

10.30602/jlk.v1i2.147 ◽

2018 ◽

Vol 1 (2) ◽

pp. 114

Author(s):

Wahdaniah Wahdaniah ◽

Sri Tumpuk

Keyword(s):

Screening Test ◽

Venous Blood ◽

The Body ◽

Ethylene Diamine ◽

P Value ◽

Cross Sectional ◽

Significant Difference ◽

Ethylene Diamine Tetra Acetate ◽

The Difference ◽

Index Value

Abstract: Routine blood examination is the earliest blood test or screening test to determine the diagnosis of an abnormality. Blood easily froze if it is outside the body and can be prevented by the addition of anticoagulants, one of which Ethylene Diamine Tetra Acetate (EDTA). Currently available vacuum tubes containing EDTA anticoagulants in the form of K2EDTA and K3EDTA. K3EDTA is usually a salt that has better stability than other EDTA salts because it shows a pH approaching a blood pH of about 6.4. The purpose of this research is to know the difference of erythrocyte index results include MCH, MCV and MCHC using K3EDTA anticoagulant with K2EDTA. This research is a cross sectional design. This study used venous blood samples mixed with K2EDTA anticoagulant and venous blood mixed with K3EDTA anticoagulants, each of 30 samples. Data were collected and analyzed using paired different test. Based on data analysis that has been done on MCH examination, p value <0,05 then there is a significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value. Then on the examination of MCV and MCHC obtained p value <0.05 then there is no significant difference between samples with K3EDTA anticoagulant with K2EDTA to erythrocyte index value.Abstrak: Pemeriksaan darah rutin merupakan pemeriksaan darah yang paling awal atau screening test untuk mengetahui diagnosis suatu kelainan. Darah mudah membeku jika berada diluar tubuh dan bisa dicegah dengan penambahan antikoagulan, salah satunya Ethylene Diamine Tetra Acetate (EDTA). Dewasa ini telah tersedia tabung vakum yang sudah berisi antikoagulan EDTA dalam bentuk K2EDTA dan K3EDTA. K3EDTA biasanya berupa garam yang mempunyai stabilitas yang lebih baik dari garam EDTA yang lain karena menunjukkan pH yang mendekati pH darah yaitu sekitar 6,4. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan hasil indeks eritrosit meliputi MCH, MCV dan MCHC menggunakan antikoagulan K3EDTA dengan K2EDTA. Penelitian ini merupakan penelitian dengan desain cross sectional. Penelitian ini menggunakan sampel darah vena yang dicampur dengan antikoagulan K2EDTA dan darah vena yang dicampur dengan antikoagulan K3EDTA, masing-masing sebanyak 30 sampel. Data dikumpulkan dan dianalisis menggunakan uji beda berpasangan. Berdasarkan analisis data yang telah dilakukan pada pemeriksaan MCH didapatkan nilai p < 0,05 maka ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit. Kemudian pada pemeriksaan MCV dan MCHC didapatkan nilai p < 0,05 maka tidak ada perbedaan yang signifikan antara sampel dengan antikoagulan K3EDTA dengan K2EDTA terhadap nilai indeks eritrosit.

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