scholarly journals THE STUDY OF PHENOLIC COMPOUNDS THE LEAVES AND SHOOTS OF ROSEMARY (ROSMARINUS OFFICI-NALIS L.), INTRODUCED IN BOTANICAL GARDEN OF PYATIGORSK MEDICAL-PHARMACEUTICAL INSTITUTE

2018 ◽  
pp. 199-207 ◽  
Author(s):  
Залина (Zalina) Маирбековна (Mairbekovna) Тохсырова (Tokhsirova) ◽  
Иван (Ivan) Викторович (Viktorovich) Попов (Popov) ◽  
Ольга (Ol'ga) Ивановна (Ivanovna) Попова (Popova)
Keyword(s):  
Phenolic Compounds ◽  
Rosmarinic Acid ◽  
Raw Materials ◽  
Botanical Garden ◽  
Rosmarinus Officinalis ◽  
Mineral Waters ◽  
Biologically Active ◽  
Northern Caucasus ◽  
North Caucasus ◽  
Pharmaceutical Institute

The object of the study was the leaves and shoots of rosemary medicinal (Rosmarinus officinalis L.), introduced for 9 years in the conditions of Caucasian Mineral Waters (North Caucasus) in the Botanical garden of the Pyatigorsk medical and pharmaceutical Institute in the open ground with shelter for the winter. Preliminary identification of phenolic compounds in the extracts from leaves and shoots of rosemary were carried out by thin-layer chromatography (TLC), and found gallic acid, rosmarinic acid, quercetin and apigenin.The method of high-performance liquid chromatography (HPLC) at wavelengths of 254 and 330 nm in leaves and shoots rosemary drug found 10 compounds: flavonoids: catechin, epicatechin, quercetin, apigenin; phenolcarboxylic acids: caffeic, gallic, chlorogenic, ferulic, rosmarinic as well as ascorbic acid. The content of rosmarinic acid in terms of dry raw materials in rosemary leaves was 0.181–0.184%; in shoots 0.062–0.064%.The area of the Northern Caucasus can be considered as a promising area for cultivation of medicinal plants – rosemary medicinal (Rosmarinus officinalis) – for the production of medicinal plant raw materials with a high content of biologically active substances.

scholarly journals The content of macro- and microelements in plants of the genus Artemisia under conditions of introduction in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine

2021 ◽  
Vol 17 (3) ◽  
pp. 199-209
Author(s):  
O. A. Коrablova ◽  
D. B. Rakhmetov ◽  
M. I. Shanaida ◽  
O. M. Vergun ◽  
Т. S. Bagatska ◽  
...  
Keyword(s):  
Mineral Composition ◽  
Aerial Part ◽  
Toxic Elements ◽  
Raw Materials ◽  
Botanical Garden ◽  
Biologically Active ◽  
Practical Significance ◽  
Artemisia Dracunculus ◽  
Plant Raw Materials ◽  
Pharmaceutical Industries

Purpose. To determine mineral composition of plants of species of the genus Artemisia (A. dracunculus L., A. abrotanum L. and A. argyi H.Lév. & Vaniot) to determine the possibility of their safe use in the food and pharmaceutical industries of Ukraine. Methods. X‑ray fluorescence method for determining the elemental composition of plant raw materials. Results. The content of mineral elements in plants of the genus Artemisia was determined depending on their ability to absorb elements from the soil and accumulate in the roots and organs of the aerial part was determined. The quantitative content of 21 macro- and microelements was established, and some peculiarities of their migration and distribution in the soil – roots – plant system were revealed. In particular, the aerial part of the studied plants contains K, Fe, Cu, Zn and Mn, which are the most important elements in the life of the plant organism. The content of mesoelements Ca and S is quite high. The amount of toxic elements Pb, Sr and Zr in plants is insignificant. Elements K and S accumulate in the aerial part. The content of elements in the aerial part of plants by decreasing their concentration can be represented in the form of the following series: for Artemisia dracunculus – S> K> Ca> Cl> Fe> Sr> Zn> Mn> Cu> Zr> Rb> Br> Cr; for A. abrotanum – K> Ca> S> Cl> Fe> Zn> Sr> Mn> Cu> Br> Cr> Co> Zr> Rb> Ni; for A. argyi – K> Ca> S> Fe> Cl> Sr> Zn> Mn> Co> Zr> Cu> Rb> Br> Se. The content of toxic elements in the studied plants was lower than the maximum allowable concentrations for plant raw materials and food. Conclusions. For the first time under conditions of introduction in the M. M. Gryshko National Botanical Garden of the NAS of Ukraine in the plants Artemisia dracunculus, A. abrotanum and A. argyi the content of macro- and microelements, which are directly related to the metabolism of biologically active compounds, was determined. The peculiarities of their distribution by plant organs during the transition from soil to aboveground mass have been clarified. The obtained results can be used to evaluate and compare the quality of plant raw materials of the genus Artemisia, to determine the pharmacological properties of these plants associated with some elements of the mineral composition, and their use in the medical and food industries. The obtained data have both scientific and practical significance in the selection of economically valuable plant species for the enrichment of the cultivated flora of Ukraine.

scholarly journals Prunus cerasifera Ehrh. fruits are a source of biologically active substances and high-quality feedstock for Food for Specific Health Use

2021 ◽  
Vol 282 ◽  
pp. 04008
Author(s):  
E.V. Dunaevskaya ◽  
V.M. Gorina ◽  
E.A. Melkozerova ◽  
Kh.M. Кhеtagurov ◽  
A.Kh. Kozyrev
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Compounds ◽  
Raw Materials ◽  
Biologically Active ◽  
Prunus Cerasifera ◽  
Complex Source ◽  
The Russian Federation ◽  
Active Substances ◽  
Specific Health ◽  
Pectin Substances

From the Southern Coast collection of Prunus cerasifera Ehrh. the fruits cultivars of the Nikitsky Gardens’ breeding Medovaya, Olenka, Rumyanaya Zorka and Idilliya were distinguished by their high taste qualities and the content of BAS. According to the norms of the Russian Federation, the fruits of these cultivars belong to the group of products with a high content of potassium and cuprum; satisfactory content of magnesium and ferrum, pastilles and jams from them belong to the group of products with a satisfactory content of potassium and cuprum. Cherry plum fruits, especially dark-colored ones, are a natural complex source of antioxidants, characterized by a high content of phenolic compounds and cuprum, and a satisfactory content of ascorbic acid and zinc. The content of pectin substances in the studied cultivars is comparable to that in apples. The fruits of the Idyllia cultivar with dark-colored pulp are distinguished by the maximum accumulation of pectins - 1.14%, ascorbic acid - 9.3, phenolic compounds – 441 mg/100 g, potassium – 11239, magnesium - 464, ferrum -10.81, zinc - 10.23, cuprum - 4.17, manganese - 0.228 mg/kg. Cherry plum fruits are characterized by a high content of BAS and are suitable for use in health nutrition both in fresh form and as raw materials for Food for Specific Health Use.

scholarly journals ON THE CONTENT OF PHENOLIC COMPOUNDS IN THE CONCENTRATIONS OF THE TAGETES PATULA L.

2018 ◽  
pp. 91-98
Author(s):  
Надежда (Nadezhda) Михайловна (Mikhajlovna) Червонная (Chervonnaya) ◽  
Ольга (Ol'ga) Андреевна (Аndreevna) Андреева (Andreeva) ◽  
Симилла (Similla) Леонтьевна (Leont'evna) Аджиахметова (Adzhiakhmetova) ◽  
Эдуард (Eduard) Тоникович (Tonikovich) Оганесян (Oganesian)
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Alcohol ◽  
Raw Materials ◽  
Total Content ◽  
Botanical Garden ◽  
Uv Spectrophotometry ◽  
Tagetes Patula ◽  
Phenol Composition ◽  
Pharmacological Screening ◽  
Pharmacologically Active

The purpose of this work is to identify the phenolic compounds of the marigold inflorescences, as well as the quantitative determination of flavonoids in the extraction obtained by extracting inflorescences with 40% ethanol.Earlier, with the purpose of preliminary assessment and subsequent selection of the fraction obtained by treating the feed with ethyl alcohol of different concentrations, the total content of antioxidants in alcohol-aqueous extracts from the inflorescences of marigolds was studied. In parallel, pharmacological screening confirmed that the most pronounced antioxidant, wound-healing and endothelioprotective activity is extracted, the alcohol obtained by ethanol 40%.In this paper, the results of the phenol composition of the most pharmacologically active fraction obtained by extracting ethyl alcohol 40% of marigolds spread out are presented. The raw materials – the inflorescences of marigolds of the propagated varieties "Carmen" were collected in September 2016 in the botanical garden of the Pyatigorsk Medical and Pharmaceutical Institute, a branch of of FGBOU in VolgGMU Ministry of Health of Russia.In the analyzed extract, 4 substances of polyphenol nature – patuletin, patulintrin, quercetin and caffeic acid – were identified. Analytical characteristics of substances were determined by physicochemical methods.Using UV spectrophotometry using the value of the specific absorption index, the quantitative content of the sum of flavonoids in terms of patuletin is determined and is 2,98 ± 0,06%. The methodology is validated by such indicators as specificity, linearity, accuracy, precision.

scholarly journals Use of Capillary Electrophoresis Method in Determination of Quantitative Content of Mangiferin in Grass of Species of Genus Hedysarum (Hedysarum Caucasicum M.Bieb., Hedysarum Grandiflorum Pall., Hedysarum Daghestanicum Rupr. ex Boiss) of flora of the North Caucasus

2021 ◽  
Vol 10 (1) ◽  
pp. 90-96
Author(s):  
D. R. Imachueva ◽  
F. K. Serebryanaya
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Determination ◽  
Botanical Garden ◽  
Biologically Active ◽  
North Caucasus ◽  
The North ◽  
Capillary Electrophoresis Method ◽  
Quantitative Content ◽  
Electrophoresis Method

Introduction. The species of the genus Hedysarum L. of the Fabaceae family are of significant interest in connection with the accumulation of a group of biologically active substances with antibacterial and antiviral activity against DNA-containing viruses.Aim. The purpose of the work is to develop a method for the quantitative determination of mangiferin in the aboveground part of species of the genus Hedysarum L., Hedysarum caucasicum M.Bieb., Hedysarum grandiflorum Pall., Hedysarum daghestanicum Rupr. ex. Boiss. collected in the North Caucasus by zonal capillary electrophoresis.Materials and methods. The objects of the study were species of the genus Hedysarum L., namely Hedysarum caucasicum M.Bieb., Hedysarum grandiflorum Pall., Hedysarum daghestanicum Rupr. ex. Boiss. collected in the North Caucasus. Registration of electronic spectra was carried out on capillary electrophoresis "KAPEL'®-105m" (OJSC "Lumex-marketing", Russia) with quartz capillary Leff./Ltol. = 50/60 cm, ID = 75 μm. As a standard sample, mangiferin (substance-powder, mangiferin content ≥98 %, Sigma-Aldrich, Lot SLBP4044V) was used.Results and discussion. As a result of comprehensive pharmacognostic studies of species of the kopecki genus growing in the North Caucasus under natural conditions, as well as under the conditions of introduction on the territory of the Botanical Garden of the Pyatigorsk Medical and Pharmaceutical Institute and the Mountain Botanical Garden of the Dagestan Federal Research Center of the Russian Academy of Sciences, we developed a method for the isolation and quantitative determination of mangiferin by capillary electrophoresis.Conclusion. Studies show that the use of the zonal capillary electrophoresis method in the analysis of xanthones, including the determination of the quantitative content of mangiferin, is promising. The largest content of mangiferin (0.25 %) is distinguished by Hedysarum caucasicum M.Bieb., which confirms the assumption based on molecular genetic studies, since it is this species that belongs to the Obscura section, as well as the alpine penny used to produce mangiferin. Therefore, it is possible to provide this technique for analysis of mangiferin xanthone glycoside along with spectrophotometry and high performance liquid chromatography.

scholarly journals Search for alternative plant raw materials for food industry and environmentally safe animal breeding

2021 ◽  
Vol 16 (1) ◽  
pp. 18-29
Author(s):  
Ekaterina Vladimirovna Solomonova ◽  
Nikolay Aleksandrovich Trusov ◽  
Tatyana Dmitrievna Nozdrina
Keyword(s):  
Food Industry ◽  
Raw Materials ◽  
Botanical Garden ◽  
Biologically Active ◽  
Taxus Baccata ◽  
Fruit Pulp ◽  
Plant Raw Materials ◽  
Plant Resource ◽  
Euonymus Europaeus ◽  
Environmentally Safe

The relevance of the search for alternative plant raw materials to enrich the diets of population throughout the world, including in the Russian Federation, is explained by global mass year-round insufficiency of biologically active nutrients in food. This work is aimed at identifying the most promising food plant species from the list, proposed on basis of long-term observations by staff of Tsytsin Main Moscow Botanical Garden of Russian Academy of Sciences (MBG RAS), by bachelors, studying in direction 06.03.01 (Biology) at Moscow State University of Food Production. The research materials (2016-2018) are fruits of hawthorns, rosehips, quince, eleutherococcuses, medlar, mahonia, spindle tree, yews, sumacs, oaks, red-fruited honeysuckles and needles of coniferous plants. Research results indicate the value of studied raw materials as a whole and its advantages in compared species within the genus of plants. Some recommendations for practical usage were made. The following things have been proposed: separation of fruit pulp and seeds before drying of fruits of Crataegus submollis and more preferable C. punctata; wide plantation cultivation of rosehips Rosa amblyotis and R. oxyodon; non-waste separate application of pericarp and seeds of Cydonia oblonga, Mespilus germanica and Mahonia aquifolium; medicinal use of fruits of Eleutherococcus sessiliflorus , and more preferable E. senticosus ; further study of cones of Taxus baccata and T. media; as a spice there is usage of fruits of Rhus typhina ; obtaining unique oils from fruit pulp of Lonicera glehnii, L . ferdinandii, L. maackii and arils of Euonymus europaeus; usage of acorns of Quercus robur, Q. rubra and needles of Picea abies, Pinus sylvestris in feed production. Thus, possibilities of intensifying plant resource usage in the food industry and agriculture were discovered, primarily due to fruits of native introduced species. It is necessary to continue search and further study of the most promising species - sources of alternative plant raw materials.

scholarly journals THE new aspects iN MoRPHoloGICAL AND anatomical DiAGNosTics of the herb monarda fistulosa l

2019 ◽  
Vol 19 (1-2) ◽  
pp. 19-26
Author(s):  
A. S Lapina ◽  
V. A Kurkin ◽  
V. M Ryzhov ◽  
L. V Tarasenko
Keyword(s):  
Raw Materials ◽  
Anatomical Study ◽  
Botanical Garden ◽  
Biologically Active ◽  
Diagnostic Features ◽  
Monarda Fistulosa ◽  
Reflected Light ◽  
Pharmacopoeial Monograph ◽  
New Type ◽  
Stems And Leaves

Topicality. The herb of Monarda fistulosa L. cultivated in the Russian Federation is one of the promising sources of essential oils, flavonoids and other biologically active compounds. The problem of standardization of raw materials of this plant is not fully resolved. Objective. The article is aimed at specification and addition of anatomical and histological signs of leaves and stems of Monarda fistulosa, as well as at the study of the luminescent features of their tissues. Materials and methods. The material of the study was the stems and leaves of the Monarda fistulosa, collected in the flowering period in July 2017 in the Botanical Garden of Samara University. The experiment was carried out by the method of light microscopy in transmitted and reflected light on a light field in accordance with the requirements of the monograph of the State Pharmacopoeia of Russia, XIV edition. Results. The article presents the results of a morphological and anatomical study of the herb of Monarda fistulosa. Additionally, the diagnostic features of the studied plant were determined, and the luminescence features of the tissues of the stems and leaves were also described. Conclusion. The obtained data allow us to develop a section “Microscopic signs” of the Pharmacopoeial monograph for the new type of medicine plant material - “herb of Monardae fistulosae”.

scholarly journals EDIBLE ONION FLOWERS (ALLIUM L.) AS A SOURCE OF BIOLOGICALLY ACTIVE SUBSTANCES

2021 ◽  
pp. 291-297
Author(s):  
Tat'yana Ivanovna Fomina ◽  
Tat'yana Abdulkhailovna Kukushkina
Keyword(s):  
Phenolic Compounds ◽  
Raw Materials ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Forest Steppe ◽  
Dry Matter Content ◽  
Total Sugars ◽  
Active Substances ◽  
Pectin Substances

The results of comparative study of the content of biologically active substances in the flowers of 10 species of perennial onions Allium aflatunense B. Fedtsch., A. altaicum Pall., A. flavum L., A. microdictyon Prokh., A. nutans L., A. obliquum L., A. ramosum L., A. rosenbachianum Regel, A. schoenoprasum L., A. senescens L. var. glaucum Regel (A. senescens ssp. glaucum (Schrad.) N. Friesen) are presented. The freshly collected raw materials in the phase of mass flowering were analyzed. The dry matter content was determined by drying 1 g of raw material at 100–105 °C to a constant mass. The amount of phenolic compounds, pectin substances, total sugars, and carotenoids was determined spectrophotometrically using SF-56 (Russia) and SF "Agilent" 8453 (USA). For the first time, data on the quantitative content of the main groups of secondary metabolites in onion flowers were obtained. It has been established that in the forest-steppe of Western Siberia, flowers of Allium species contain: 10.7-22.5% dry substances, 6.6-26.2% total sugars, 3.3–18.6% pectin substances, 3.6–10.6% phenolic compounds, 3.0–23.4  mg% carotenoids (per mass of absolutely dry materials) and 35.4–157.8 mg% ascorbic acid (per mass of raw materials). The highest values were found for A. rosenbachianum, A. flavum, and A. aflatunense. The high variability of the accumulation of biologically active substances is due to the species and the influence of weather conditions during the growing season. The study showed the prospects for using onion flowers as a source of various bioactive compounds, including when added to food.

scholarly journals The Effect of Polyphenolics in Extracts from Natural Materials on Metabolic Activity of Metastatic Melanoma WM-266-4 Cells

2020 ◽  
Vol 10 (10) ◽  
pp. 3499 ◽  
Author(s):  
Taja Žitek ◽  
Barbara Dariš ◽  
Matjaž Finšgar ◽  
Željko Knez ◽  
Dragana Bjelić ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Metabolic Activity ◽  
Raw Materials ◽  
Curcuma Longa ◽  
Rosmarinus Officinalis ◽  
Biologically Active ◽  
Total Phenolic ◽  
Equisetum Arvense ◽  
Lycium Barbarum ◽  
Materials Used

The importance of natural crops in medicine and pharmacy is growing. Beside bioactive compounds used directly as therapeutic agents, there are also raw materials used for drug synthesis or as a basic model for new biologically active compounds. In this paper, the optimum conditions for material extraction of Curcuma longa, Lycium barbarum, Equisetum arvense, Vitis vinifera, and Rosmarinus officinalis were investigated to achieve high antioxidant levels. The main aim of this study was to verify the correlation between the content of antioxidants, proanthocyanidins and total phenolic substances for certain extracts from the raw materials (Curcuma longa, Lycium barbarum, Equisetum arvense, Vitis vinifera and Rosmarinus officinalis) and the reduction of the metabolic activity of skin cancer cells.

scholarly journals PERSPECTIVES FOR THE PRODUCTION OF COSMETIC OILS BASED ON THE PLANT COMPIONENTS

2020 ◽  
pp. 101-102
Author(s):  
Nino Guleishvili ◽  
Manana Gabidzashvili ◽  
Inga Bochoidze
Keyword(s):  
Refractive Index ◽  
Phenolic Compounds ◽  
Extraction Method ◽  
Raw Materials ◽  
Biologically Active ◽  
Total Flavonoids ◽  
Total Phenols ◽  
Essential Nutrients ◽  
Natural Plant ◽  
Plant Components

The use of the natural plant components (fatty oils) and biologically active additives allows for balancing the formulation for cosmetic oils. When using biologically active supplements, the skin is rich in all the necessary nutrients that help to achieve the desired effect. When using biologically active additives, the skin is enriched with all the essential nutrients that help to achieve the desired effect. Research covered rosehip fruits wild-growing in western Georgia. To obtain extract from raw materials, we used the superfluid CO2 extraction method. We obtained oil with yield ranging from 8.12 to 10.17%, whose refractive index is 1,4782 units, and the extract rich in phenolic compounds, with total phenols -3032 mg/100 g and total flavonoids 1501 mg/100 g on dried basis.

scholarly journals THE STUDY OF ANTIOXIDANT ACTIVITY OF THE HERB ASTRAGALUS GLYCYPHYLLUS L

2019 ◽  
Vol 19 (1-2) ◽  
pp. 27-32
Author(s):  
T. A Pozdnyakova ◽  
R. A Bubenchikov
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Phenolic Compounds ◽  
Raw Materials ◽  
Natural Antioxidants ◽  
Hydroxycinnamic Acids ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Chain Reactions ◽  
Active Substances

Topicality. In the process of originating and development of many diseases, an important role belongs to free radicals, which trigger chain reactions leading to damage of cells and tissues. The natural antioxidants can help to neutralize free radicals, among which phenolic compounds are the most active. The presence of these groups of biologically active substances in the herb Astragalus glycyphyllus L. suggests the presence of antioxidant activity in the phyto-complexes of the studied plant. The purpose of this research was to study the antioxidant activity of extracts from the herb Astragalus glycyphyllus L. Materials and methods. The object of the study was the air-dry herb Astragalus glycyphyllus L. harvested in the period of mass flowering. To obtain the most reliable results of the study, the investugation of the antioxidant activity of extracts from the herb Astragalus glycyphyllus L. was carried out by two met hods: by the method based on the chemical reaction between potassium permanganate and regenerating biologically active substances contained in extracts from the studied plant and by spectrophotometry of free radicals based on the reaction interactions of 2,2-diphenyl-1-picrylhydrazyl with antioxidants contained in plant raw materials. To establish the dependence between the antioxidant activity and the presence of phenolic compounds in the plant, the content of flavonoids and hydroxycinnamic acids in Astragalus glycyphyllus L. was determined. Results. It was found that all the studied extracts from the herb Astragalus glycyphyllus L. have antioxidant activity, but their values are different depending on the used extractant. In this case, a consistent pattern is observed between the content in the extraction of phenolic compounds (hydroxycinnamic acids and flavonoids) and the magnitude of antioxidant activity. Conclusions. As a result of the conducted research, the total antioxidant activity of extracts from the herb Astragalus glycyphyllus L. was established. Its value was found to be related to the content of phenolic compounds in the plant by a positive correlation. The obtained data indicate the promising use of the herb Astragalus glycyphyllus L. as a source of natural antioxidants. · Keywords: Astragalus glycyphyllus L.; antioxidant activity; flavonoids; hydroxycinnamic acids; free radicals.

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