Trigonella Foenum Graecum Extract Benefits on Hematological, Biochemical and Male Reproductive System of as a Complementary Therapy with Glimepiride in Treating Streptozotocin Induced Diabetic Rats

Diabetes mellitus (DM) is a chronic metabolic disorder. Streptozotocin is a naturally occurring cytotoxic chemical, particularly toxic to the pancreas and insulin producing beta cells in mammals and induces diabetes. Glimepiride is a second generation sulfonylurea, used as second-line or add-on treatment options for type 2 diabetes. Fenugreek (Trigonellafoenum graecum) seeds have been documented as a traditional plant treatment for diabetes. Soluble dietary fiber of Fenugreek significantly improved oral glucose tolerance in diabetic rats. It also exerts anti-diabetic effects mediated through the inhibition of carbohydrate digestion and absorption and the enhancement of peripheral insulin action. Most herbal remedies can interact with allopathic drugs resulting in altered activity and toxicity. At the same time, herbal remedies might produce the same kind of effects as the drug produce. Current published research information on herb-drug interactions is scanty. So, the aim of this study was to investigate the possible interaction between conventional drug used for the management of diabetes; (Glimepiride) and a traditional herbal remedy; Fenugreek aqueous extract in Streptozotocin induced diabetic male albino rats. In conclusion, combination therapy induces better hematological, biochemical effects and improves the oxidative stress biomarkers and antioxidant enzymes. Histological studies showed better results on some organ functions. The results emphasize the benefit of using the combination of Fenugreek seeds aqueous extracts as supportive complementary anti-diabetic therapy.

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The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽

10.3390/molecules26051332 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1332

Author(s):

Gilda M. Iova ◽

Horia Calniceanu ◽

Adelina Popa ◽

Camelia A. Szuhanek ◽

Olivia Marcu ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Diabetic Rats ◽

Gingival Tissue ◽

Control Group ◽

Albino Rats ◽

Oxidative Stress Biomarkers ◽

Significant Difference ◽

The Impact ◽

Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

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Beneficial Effects of Fennel (Foeniculum Vulgare) in Treating Obesity in Rats

Journal Of Obesity Management ◽

10.14302/issn.2574-450x.jom-18-2484 ◽

2019 ◽

Vol 1 (2) ◽

pp. 16-33 ◽

Cited By ~ 2

Author(s):

Nawal A. A. Elghazaly ◽

Eman H. Radwan ◽

Hala H. Zaatout ◽

Mohamed M. Elghazaly ◽

Nour El din Allam

Keyword(s):

Total Protein ◽

Histopathological Examination ◽

Insulin Resistance Syndrome ◽

Serum Levels ◽

Herbal Remedies ◽

Control Group ◽

Albino Rats ◽

Oxidative Stress Biomarkers ◽

Foeniculum Vulgare ◽

Beneficial Effects

Obesity is associated with a number of serious medical complications, which are often referred to as the “insulin resistance syndrome”. The aim of the present study was performed to investigate the possible interaction between a conventional drug used for management of cholesterol and traditional herbal remedies on the obesity. This was carried through out: through estimation of blood test; Estimation of serum tests; Determination of oxidative stress biomarkers and the antioxidant enzymes activities in the liver were assayed; Histopathological examination of the liver and kidney of adult male albino rats were done. In the present study, the serum levels of the total protein and albumin in the obesity group (7.1± 0.2) and (4.78 ± 0.19); respectively were significantly (p ≤ 0.05) more than those of the control group (6.5±0.1) and (3.95± 0.1).The administration of (fennel group) revealed significant (P<0.05) decrease in the serum levels of the albumin and total protein (4.38± 0.1) and (6.65± 0.2); respectively as compared to the obesity group (4.78 ± 0.19) and (7.1± 0.2(. The total cholesterol of the group(5) (fennel and ator) after two weeks from a high fat diet than treatment with fennel and Ator through six weeks equal 142.86±5.9, 100.4±8.68, 93.29±5.99, 87.1±11.28, 80.4±21.55, 78.1±6.7 and 77.1±6.87; respectively. The present study showed a significant (P<0.05) increase in the activities of ALT, AST and ALP in the obesity group which recorded as (60.5±11.45), (57.25±6.3) and (845.0±49.47); respectively as compared to the control group (28.25±1.7), (38.5±3.87) and (537.0±41.5); respectively. The fennel group caused significant decrease in the activities of these enzymes (41.0± 2.9), (42.25+3.2) and (717.75+48.6); respectively compared to the obesity group. Ator group showed a significant decrease in the activities of these enzymes (40.0±2.16), (42.5±3.1) and (679.25±41.16); respectively compared as obesity group. The activity of AlT, AST and ALP in the fennel and ator group (32.75±2.5), (40.5±2.38) and (601.25±17.5); respectively were near to the control group.

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Effect of administration of methanol root bark extract of Cussonia arborea on serum lipid profile and oxidative biomarker parameters in alloxan-induced diabetic rats

Indian Journal of Animal Research ◽

10.18805/ijar.9555 ◽

2016 ◽

Author(s):

Patrick Emeka Aba ◽

Isaac Uzoma Asuzu

Keyword(s):

Oxidative Stress ◽

Total Cholesterol ◽

Serum Lipid ◽

Methanol Extract ◽

Diabetic Rats ◽

Bark Extract ◽

Albino Rats ◽

Root Bark ◽

Oxidative Stress Biomarkers ◽

And Oxidative Stress

This study investigated the effects of methanol extract of Cussonia arborea on serum lipid and oxidative stress biomarkers of alloxan-induced diabetic rats. A total of 72 male albino rats assigned into 6 groups of 12 rats per group were used. Groups 1-5 were made diabetic while group 6 were normal. Groups 1-4 were treated with 62.5, 125, 250 mg/kg of the extract and 2 mg/kg glibenclamide respectively while groups 5 and 6 received 10 ml/kg distilled water each. Total cholesterol, triglyceride, high density lipoprotein (HDL), malondiadehyde, catalase, and superoxide dismutase (SOD) were assayed on days 28, 56 and 84 post treatment. The results indicated that the extract significantly (p<0.05) reduced the levels of total cholesterol, triglyceride, malondialdehyde but significantly (p<0.05) increased the activities of SOD, catalase and the levels of HDL when compared to negative control. It was therefore concluded that methanol extract of C. arborea mitigated dyslipidaemia and oxidative stress.

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Hypoglycemic Activity of Aqueous Root Bark ExtractZanthoxylum chalybeumin Alloxan-Induced Diabetic Rats

Journal of Diabetes Research ◽

10.1155/2016/8727590 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 3

Author(s):

Moses Solomon Agwaya ◽

Peter California Vuzi ◽

Agnes Masawi Nandutu

Keyword(s):

Blood Glucose ◽

Treatment Options ◽

Diabetic Rats ◽

Bark Extract ◽

Initial Increase ◽

Oral Glucose ◽

Root Extract ◽

Root Bark ◽

Significant Difference ◽

Alloxan Monohydrate

Background. Medicinal plants offer cheaper and safer treatment options to current diabetic drugs. The present study evaluated the effect of aqueous root bark extract ofZanthoxylum chalybeumon oral glucose tolerance and pancreas histopathology in alloxanized rats.Method. Diabetes was induced in rats by administration of alloxan monohydrate. Root extract ofZ. chalybeumwas administered to rats at 200 and 400 mg/kg BW daily for 28 days. Blood glucose was measured by glucometer and pancreatic histopathology evaluated microscopically.Results. Initial increase was observed in blood glucose of the rats after oral administration of glucose from time zero. Two hours after treatment withZ. chalybeum, a significant reduction in blood glucose was observed within treatment groups (p<0.05) compared to 0.5 hr and 1 hr. There was no significant difference between treatment group receiving 400mg/Kg BW extract and the normal groups (p=0.27), implying that the former group recovered and were able to regulate their blood sugar, possibly via uptake of glucose into cells. The reversal in pancreatic histopathology further supports the protective effect ofZ. chalybeumextract towards diabetic damage.Conclusion. Extract ofZ. chalybeumis effective in controlling blood glucose in diabetes and protecting pancreatic tissues from diabetic damage.

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Metformin Improves Biochemical and Pathophysiological Changes in Hepatocellular Carcinoma with Pre-Existed Diabetes Mellitus Rats

Pathogens ◽

10.3390/pathogens10010059 ◽

2021 ◽

Vol 10 (1) ◽

pp. 59

Author(s):

Maysa A. Mobasher ◽

Mousa O. Germoush ◽

Hala Galal El-Tantawi ◽

Karim Samy El-Said

Keyword(s):

Diabetes Mellitus ◽

Hepatocellular Carcinoma ◽

Malignant Tumors ◽

Diabetic Rats ◽

Albino Rats ◽

Sprague Dawley ◽

Oxidative Stress Biomarkers ◽

Liver Cancers ◽

Liver Functions ◽

Before And After

Hepatocellular carcinoma (HCC) is one of the world’s most widely recognized malignant tumors that accounts for 90% of all the primary liver cancers and is a major cause of death from cancer, representing half a million deaths per year. Obesity and associated metabolic irregularities, particularly diabetes mellitus (DM) and insulin resistance, are important risk factors for the advancement of HCC. Recently, retrospective studies showed that metformin (MET) could protect the hepatic tissues in pre-existing diabetes mellitus from HCC. The purpose of this study was to assess the role of MET treatment in the pre-existing diabetic rats before and after HCC induction by diethylnitrosamine (DEN). Thirty-five male Sprague Dawley albino rats were partitioned into the following groups: Group 1 (Gp1) was the control. Gp2 was injected intraperitoneally (i.p) with streptozotocin (STZ) (80 mg/kg) and DEN (50 mg/kg/7 weeks). Gp3, Gp4, and Gp5 were injected as in Gp2 and treated with MET (150 mg/kg) before and/or after HCC induction. Biochemical parameters including liver functions, lipid profile, and oxidative stress biomarkers were determined. Furthermore, histological and immunohistochemical changes were assessed in all groups. Our results illustrated that the group of rats that were treated with STZ and DEN had significant changes in both liver functions and were associated with alterations in the liver histopathological architectures. Treatment with MET before or after HCC induction ameliorated the cellular changes in the liver tissues; however, the utmost protection was found in a group of rats, which were treated with MET before and after HCC induction.

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Altered Lipid Responses to Dietary Interventions in Obesity

Current Research in Nutrition and Food Science Journal ◽

10.12944/crnfsj.3.1.01 ◽

2015 ◽

Vol 3 (1) ◽

pp. 01-11

Author(s):

Meena Godhia ◽

Nagma Naik

Keyword(s):

Vitamin D ◽

Body Weight ◽

Hematological Parameters ◽

Fasting Blood Glucose ◽

Vitamin D Supplementation ◽

Diabetic Rats ◽

Albino Rats ◽

Oral Glucose ◽

Hematological Analysis ◽

Glycemic Profile

Results obtained from studies on the effect of vitamin D supplementation with or without calcium on glucose homeostasis and hematological parameters have been inconsistent. This experimentally-controlled designed study investigated the combined effects of Ca2+ and Vit.D-fortified diet on body weight, glycemic profile, biochemical, haemostatic and haematological parameters in 2 groups (n=8, each) of experimental male diabetic and healthy albino rats following treatment with Ca2+ and Vit.D-fortified diet for 6 weeks. 2 similar groups of rats (n=8, each) on normal diets served as normal and diabetic controls respectively to allow comparison between groups. Induction of diabetes (100mg/dL, intraperitoneally) was achieved with freshly prepared alloxan monohydrate solution after 15 hours overnight fast while oral glucose tolerance test, biochemical and hematological analysis were performed on blood samples. Fasting blood glucose (FBG) was taken at study baseline and 6 weeks after feeding. Mean weights were significantly (p < 0.05) lower in calcium/vitamin D-fortifed diet-fed diabetic and normal rats compared with their respective controls. Actual percentage numerical weight gain at 6 weeks of study includes: diabetic rats on treatment diet (15.50%); diabetic controlled rats (18.70%); normal rats on treatment diet (20.40%); normal controlled rats (25.10%). At 6 weeks of study, experimental diabetic rats showed significant (p < 0.05) reduction (22.83%) in mean FBG concentration compared with the diabetic control rats. Experimental rats fed on calcium and vitamin D-fortified diet displayed improved glycemic tolerance over their respective controls. Hematological analysis revealed insignificant (p > 0.05) difference in hematological and hemostatic indices between the experimental and controlled rats. In diabetic rats, Ca2+ and Vit.D-fortified diet reduced body weight with beneficial hypoglycemic and remarkable glycemic tolerant effects on glycemic profile without significant impact on hemostatic and hematological indices.

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Effects of Calcium and Vitamin D-Fortified Diet on Glycemic Profile, Biochemical Parameters and Selected Haemostatic and Haematological Indices in Diabetic Rats

Current Research in Nutrition and Food Science Journal ◽

10.12944/crnfsj.3.1.02 ◽

2015 ◽

Vol 3 (1) ◽

pp. 12-19

Author(s):

Magnus Anyakudo ◽

Adedoyin Adebukola

Keyword(s):

Vitamin D ◽

Body Weight ◽

Hematological Parameters ◽

Fasting Blood Glucose ◽

Vitamin D Supplementation ◽

Diabetic Rats ◽

Albino Rats ◽

Oral Glucose ◽

Hematological Analysis ◽

Glycemic Profile

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ANTI HYPERGLYCEMIC EFFECT OF OCIMUM SANCTUM, TRIGONELLA FOENUM GRAECUM, CURCUMA LONGA BY ORAL GLUCOSE TOLERANCE AND ORAL STARCH TOLERANCE STUDY IN ALBINO RATS

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2020.12.01.035 ◽

2020 ◽

Vol 12 (01) ◽

Keyword(s):

Glucose Tolerance ◽

Curcuma Longa ◽

Ocimum Sanctum ◽

Albino Rats ◽

Oral Glucose ◽

Oral Glucose Tolerance ◽

Trigonella Foenum Graecum ◽

Tolerance Study ◽

Hyperglycemic Effect

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Antidiabetic Effects of Ethanolic Extract of Ficus glomerata (L.) Roots

Current Bioactive Compounds ◽

10.2174/1573407215666190401151405 ◽

2020 ◽

Vol 16 (1) ◽

pp. 33-41

Author(s):

Mohini C. Upadhye ◽

Uday Deokate ◽

Rohini Pujari ◽

Vishnu Thakare

Keyword(s):

Body Weight ◽

Glucose Tolerance ◽

Density Lipoprotein ◽

Ethanolic Extract ◽

Tolerance Test ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Control Group ◽

Oral Glucose ◽

Ficus Glomerata

Background: Ficus glomerata (F. glomerata) Linn. Family Moraceace is a large tree found all over India including outer Himalayan ranges, Punjab, Chota Nagpur, Bihar, Orissa, West Bengal, Rajasthan, Deccan and also as a common plant in South India. It is planted around the home and temples. It is cultivated throughout the year, distributed in evergreen forests and moist localities. Objective: The Ethanolic Extract of roots of F. Glomerata (EEFG) belonging to the family Moraceace, was investigated for its antidiabetic activity using alloxan induced diabetic rats. Methods: Thirty rats were divided into 5 groups having 6 rats in each group. The alloxan was administered to the rats of all groups except normal control group through intraperitoneal route at a concentration of 140mg/kg body weight. A dose of 100mg/kg and 200 mg/kg body weight of EEFG was administered to alloxan induced diabetic rats. The administration of the extract was lasted for 11 days. Effectiveness of the extract on glucose, cholesterol, triglycerides, and high density lipoprotein and protein concentrations was analyzed. Results: Significant (p<0.05) reduction in the levels of glucose, cholesterol, triglyceride of the diabetic rats was observed after treatment with ethanolic extract. After subjecting to oral glucose tolerance test EEFG also showed significant improvement in glucose tolerance. Conclusion: F. glomerata root ethanolic extract showed that it possesses antidiabetic effect and can be found useful for the management of diabetes mellitus.

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Flavonoids Extracted from Asteriscus graveolens Improve Glucose Metabo-lism and Lipid Profile in Diabetic Rats

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320999200818103709 ◽

2020 ◽

Vol 20 ◽

Author(s):

Fadwa El-ouady ◽

Fatima Bachir ◽

Mohamed Eddouks

Keyword(s):

Glucose Tolerance ◽

Lipid Profile ◽

Histopathological Examination ◽

Oral Treatment ◽

Hdl Cholesterol ◽

Diabetic Rats ◽

Oral Glucose ◽

Traditional Use ◽

Soxhlet Apparatus

Aim: This study aimed to evaluate the antidiabetic and antihyperlipidemic effects of Asteriscus graveolens. Background: Asteriscus graveolens (Asteraceae) is a medicinal plant widely used by the Moroccan population to treat various diseases including diabetes. Objective: This work aimed to assess the capacity of flavonoids extracted from Asteriscus graveolens (FEE) to improve diabetes mellitus and dyslipidemia in normal and STZ-induced diabetic rats. Methods: Flavonoids were extracted from A. graveolens using the Soxhlet apparatus and using different organic solvents. Normal and streptozotocin-induced diabetic rats were treated orally by the extract of A. graveolens at a dose of 10 mg/kg. The oral treatment during 15 days was used to evaluate the effect of the flavonoids extracted from A. graveolens on blood glucose level and lipid profile in normal and diabetic rats. The oral glucose tolerance test as well as the analysis of histopathological examination of liver was performed. The antioxidant activity of FEE was also assessed by the method of trapping of free radical 2,2-diphenyl-1 picrylhydrazyl (DPPH), in order to estimate the mechanisms of action involved by FEE to improve hyperglycemia and lipid profile in normal and diabetic rats. Results: FEE reduced serum glucose concentrations in both normal and diabetic rats and exhibited in the last group lowering total cholesterol and triglycerides effects as well as improvement of the HDL-cholesterol serum level. In addition, a remarkable influence on glucose tolerance was also noticed after FEE treatment. Moreover, FEE was able to improve histopathological status of liver and possess a potential antioxidant effect in vitro. Conclusion: In conclusion, this study demonstrates the hypoglycemic and antihyperlipidemic effects of FEE in rats supporting then its traditional use for the management of diabetes.

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