scholarly journals Colored Anti-Hail Nets Modify the Ripening Parameters of Nebbiolo and a Smart NIRS can Predict the Polyphenol Features

2021 ◽  
Vol 4 (1) ◽  
pp. 23-45
Author(s):  
Alberto Cugnetto ◽  
Giorgio Masoero

In the cultivation of vines the risk of hail appears to be increasing with the ongoing climate change. The use of protective plastic nets is spreading, but there is little knowledge on the additional processing costs and on the phenological and qualitative consequences, moreover, as depending on different colors of the nets. Thus, a randomized trial was carried out in Nebbiolo, a wine of great aging, testing three plastic anti-hail nets colored in green, gray and black. Results showed that 24% more-time was necessary for the canopy management practices under the nets. The black nets advanced the ripening process, with a berry weight decrease of about 13%. A significant decrease in the seed number per berry was observed under the black nets (-45%), while a rise (+102%) was observed under the green and gray nets with a parallel increase in the pH of the juice (+13%) and in malic acid (+28%) under the green nets. The berry skin did not show any significant differences in polyphenol and anthocyanin profiles, while the plants that matured in the upper part of the vineyard showed higher level in the berry skin extractable flavan-reactive to vanillin, and total polyphenol. On the other hand, in the seeds grown under green nets an increase in the extractable polyphenol compounds was observed, sign of a delay in seed ripening, with a higher tannin polymerization ratio, preserving the malic acid, and decreasing the acidity of the berry. In conclusion, the use of colored green may be a useful tool against excessive microclimatic warming and / or irradiation. The field internal variability affects much more the ripening dynamics than the nets used. A second aim was to develop a smart NIR SCiOTM model for polyphenols and the results were in line with the favorable expectations, providing R2 predictions of about 0.74 from the skin and 0.81 from the seeds.

2019 ◽  
Vol 10 ◽  
pp. 147-150
Author(s):  
Andi Yusniah ◽  
Yuspian Nur ◽  
Islamudin Ahmad

This study aims to determine the effect of the use of malic acid-glucose based microwave assited extraction (MAE) methods on total polyphenol extraction from kadamba leaves (Mitragyna Speciosa Korth). Samples that have been dried in the extraction using solvents (green solvent) Malic Acid-Glucose with a ratio of 1: 1 and 1: 3 g/g in several conditions including, microwave strength 50% for 10 minutes with a sample ratio and a solvent of 1:10 g/mL. Then the extract solution obtained was measured in total polyphenol levels using the folin ciocalteu (FC) reagent and the absorbance was measured at a wavelength of 791 nm using a UV-vis spectrophotometer. Based on the results of the study obtained levels of polyphenols from each condition of the solvent used is 258mg GAE/g sample (1:1 g/g solvent) and 292mg GAE /g sample (1: 3 g / g solvent). From this study it can be concluded that the method of malic acid-glucose based microwave assited extraction can be used to extract polyphenol compounds from Kadamba leaves quickly, easily and efficiently.


Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 807
Author(s):  
Dorota Gumul ◽  
Rafał Ziobro ◽  
Jarosław Korus ◽  
Marek Kruczek

Gluten-free products based on starch and hydrocolloids are deficient in nutrients and do not contain pro-health substances. Therefore, they should be enriched in raw materials naturally rich in antioxidants, especially if they are intended for celiac patients, prone to high oxidative stress. Apart from the traditionally used pseudo-cereals, seeds, vegetables and fruits, innovative substrates such as the by-product (especially in Poland) dry apple pomace could be applied. The study material consisted of gluten-free bread enriched with apple pomace. The content of individual polyphenols, the content of total polyphenol and flavonoids, and also the antioxidant potential of the bread were determined by the UPLC-PDA-MS/MS methods. It was observed that apple pomace was a natural concentrate of bioactive substances from the group of polyphenols. In summary, gluten-free bread with 5% content of apple pomace showed the highest organoleptic scores and contained high levels of phenolic compounds. The values of total phenolic content, and the amounts of flavonoids, phenolic acids and phloridzin in this bread were 2.5, 8, 4 and 21 times higher in comparison to control.


2019 ◽  
Vol 32 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Nguyen Van Thuy ◽  
Nguyen Minh Tien ◽  
Nguyen Ngoc Quy ◽  
Tri Duc Lam ◽  
Huynh Thi Kieu Linh ◽  
...  

Gomphrena celosioides Mart. is well known for its medicinal values worldwide. In this study, three extracts, viz. diethyl ether extract (DEE), ethanolic extract (EE) and aqueous extract (AE), were successively obtained from the leaves and stem of Gomphrena celosioides to determine the polyphenol and flavonoid content in this plant. A wide variety of pharmacologically active compounds such as alkaloid, flavonoid, terpenoid, saponin, tannin and polyphenol compounds were present in Gomphrena celosioides. The results of quantitative determination showed that total polyphenol content of DEE, EE and AE reached 35.35 ± 1.47, 250.17 ± 2.95 and 133.92 ± 3.17 mgGAE/g, respectively. Moreover, total flavonoid content of the DEE, EE and AE was 23.21 ± 1.87, 50.74 ± 2.32 and 27.25 ± 1.34 mgQE/g, respectively. In comparison with DEE and AE, the ethanolic extract exhibited the highest DPPH (IC50 = 13.29 ± 0.10 μg/mL) and ABTS (IC50 = 6.3 ± 0.11 μg/mL).


Metabolites ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 56 ◽  
Author(s):  
Ryo Kobori ◽  
Seiya Hashimoto ◽  
Hayato Koshimizu ◽  
Shuich Yakami ◽  
Mizuki Hirai ◽  
...  

Berry fruits are well known to contain large amounts of polyphenol compounds. Among them, flavan-3-ol derivatives are a group of secondary metabolism compounds currently attracting a great deal of attention owing to their health benefits. Not only the fruits, but also the leaves of raspberry plants, are highly esteemed for tea making around the world and are largely used for food. In this report, we discuss the results of our study on the effect of light and temperature on polyphenol accumulation in raspberry leaves. When raspberry was cultivated in a plant factory unit and light intensity, wavelength, and temperature were varied, the amount of total polyphenol increased under blue light. Quantitative determination of (+)-catechin, (–)-epicatechin, procyanidin B4, flavan-3-ol trimer, which are flavan-3-ol derivatives, was carried out using HPLC, whereby we confirmed their increase under blue light. Semi-quantitative RT-PCR showed correlation between chalcone synthase (CHS) gene expression and the amounts of the compounds measured in the leaves.


2019 ◽  
Vol 10 ◽  
pp. 118-121
Author(s):  
Kurnya Kurnya ◽  
Hajrah Hajrah ◽  
Islamudin Ahmad

Dayak Onion (Eleutherine bulbosa [Mill.] Urb.) It is a plant that grows in Indonesia precisely in the Kalimantan area and has traditionally efficacious in curing various diseases such as diabetes mellitus, hypertension, breast cancer, cholesterol-lowering, and stroke. This study aims to determine the effect of using the lactic acid-sucrose based microwave-assisted extraction (MAE) method to extract total polyphenols from Dayak onion tubers (Eleutherine bulbosa [Mill.] Urb.). Dry samples were extracted using a lactic-sucrose acid (green solvent) solvent in the ratio of 1: 1, 2: 1, and 3: 1  g/g with 50% microwave strength for 10 minutes with a ratio of samples and solvents of 1:10 g /mL. Extracts that have been obtained then determined the total polyphenol levels using a UV-VIS spectrophotometer at a wavelength of 791 nm. Based on the results of the study, it was obtained the levels of polyphenols from each condition of the solvent used in the sample that is 28.73 mg GAE/g sample ( 1: 1 g/g solvent); 20.2 mg GAE/g sample ( 2:1 g/g solvent); 36.35 mg GAE/g sample ( 3:1 g/g solvent). From this study, it can be concluded that the MAE method can be used to extract polyphenol compounds from Dayak bulbs.


Author(s):  
Tuty Anggraini ◽  
Busra Al-Hafit ◽  
Netty Sri Indeswari ◽  
Daimon Syukri

This study aimed to determine the effect of boiling time on polyphenol compounds and antioxidant activity ciplukan (leaves, stems, fruit, roots and mixture of each plant part). This research used Randomized Block Design (RBD) with five groups (leaves, stems, fruit, roots, and mixture of each part of the plant ) and three treatments (10, 20 and 30 minutes). The analysis for the experiment were the antioxidant activity of each part of the plant by using various incubation time, the antioxidant activity and total polyphenol for each treatment. The results showed that the duration of boiling had a significant effect on total polyphenols but no significant effect on antioxidant activity. The highest total polyphenols were obtained on leaves with 30 min boiling duration (1210.5 mg GAE / g), and the highest antioxidant activity was also obtained on leaves with 30 min boiling time (88.32%).


2019 ◽  
Vol 10 ◽  
pp. 114-117
Author(s):  
Kharisma Ayu Tandi Datu ◽  
Nurul Fitriani ◽  
Islamudin Ahmad

This study aim to determine the effect of use lactic acid and sucrose with assistance of Microwave Assisted Extraction (MAE) on Total Polyphenols from Herbs Suruhan (Peperomia pellucida (L.) Kunth). The dried sample of Herbs Suruhan was extracted using (green solvent) lactic acid-sucrose with a ratio of 1:1 g/g, 2:1 g/g and 3:1 g/g and the extraction conditions microwave strength was 50% for 10 minutes at a ratio sample and solvent 1:10 g/ml. Then the extract solution obtained to determine the total polyphenol level using the Folin Ciocalteu reagent and it absorbance was measured using a UV-VIS spectrophotometer at a wavelength of 791 nm. Based on the result of the study, obtained levels of polyphenols from each solvent condition used on the sample were 573,443 mg GAE/g (2:1), 472,479 mg GAE/g (1:1) and 420,14 mg GAE/g (3:1). It can be concluded that the lactic acid-sucrose method with microwave assisted extraction can be used to extract polyphenol compounds from Herbs Suruhan.


2016 ◽  
Vol 50 (7) ◽  
pp. 486-490 ◽  
Author(s):  
P. A. Struchkov ◽  
V. L. Beloborodov ◽  
I. R. Il’yasov ◽  
A. M. Savvatee ◽  
V. K. Kolkhir ◽  
...  

2016 ◽  
Vol 695 ◽  
pp. 289-294
Author(s):  
Livia Bandici ◽  
Simona Ioana Vicas ◽  
Gheorghe Emil Bandici ◽  
Alin Cristian Teusdea ◽  
Simona Cavalu

In this paper, grapes were processed by using high frequency electromagnetic field in order to obtain a high quality wine in terms of bioactive compounds content with antioxidant activity. The experimental setup comprise an applicator with adjustable power between 100-1000 W, software assisted, in order to process three varieties of grapes (Muscat Ottonel, Merlot, Pinot Noir), that were harvested in 2014 in the Crişana-Sântimreu vineyard. The total polyphenol compounds were measured using the Folin-Ciocalteu spectrophotometric method, and the antioxidant capacity was determined by the FRAP method. The results have shown that the use of high frequency electromagnetic field in grape processing leads to the diffusion of the bioactive compounds with antioxidant activity in the final product. In the same time, it is also an effective method in terms of energy consumption.


2010 ◽  
Vol 57 (2) ◽  
Author(s):  
Maria Kratchanova ◽  
Petko Denev ◽  
Milan Ciz ◽  
Antonin Lojek ◽  
Atanas Mihailov

This study investigates the influence of extraction system on the extractability of polyphenol compounds and antioxidant activity of various medicinal plants. Oxygen radical absorbance capacity (ORAC) and total polyphenol content of 25 Bulgarian medicinal plants subjected to water or 80 % acetone extractions were investigated and compared. The type of extragent significantly influenced the efficiency of the polyphenol extraction and the antioxidant activity. In all cases ORAC results and total polyphenol content were higher for acetone extraction than for water extraction. The acetone extract of peppermint had the highest ORAC value - 2917 micromol Trolox equivalent (TE)/g dry weight (DW) and polyphenol content - 20216 mg/100 g DW. For water extraction thyme exhibited the highest ORAC antioxidant activity - 1434 micromol TE/g DW. There was a significant linear correlation between the concentration of total polyphenols and ORAC in the investigated medicinal plants. It can be concluded that the solvent used affects significantly the polyphenol content and the antioxidant activity of the extract and therefore it is recommended to use more than one extraction system for better assessment of the antioxidant activity of natural products. Several of the investigated herbs contain substantial amounts of free radical scavengers and can serve as a potential source of natural antioxidants for medicinal and commercial uses.


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