scholarly journals Generation of Silver Nanoparticles by the Pin-Hole DC Plasma Source with and without Gas Bubbling

2019 ◽  
Vol 6 (2) ◽  
pp. 180-183 ◽  
Author(s):  
Z. Kozáková ◽  
F. Krčma ◽  
L. Čechová ◽  
S. Simic ◽  
L. Doskočil
Keyword(s):  
Silver Nanoparticles ◽  
Plasma Source ◽  
Input Energy ◽  
Discharge Region ◽  
Eds Analysis ◽  
Gas Bubbling ◽  
Solution Conductivity ◽  
Average Size ◽  
Silver Nitrate Solutions ◽  
Nitrate Solutions

Silver nanoparticles were produced using the pin-hole discharge generated by dc non-pulsing high voltage directly in silver nitrate solutions. Sodium nitrate was alternatively added to increase solution conductivity and decrease input energy for the discharge breakdown. Argon or oxygen was bubbled through the discharge region. Comparative experiments were evaluated by UV-VIS spectrometry. Formation of silver nanoparticles with the average size of 100 nm was confirmed by SEM/EDS analysis.

Induction period of the crystallization of silver nitrate solutions in ethylene glycol

1991 ◽  
Vol 56 (10) ◽  
pp. 2142-2147
Author(s):  
Ivo Sláma
Keyword(s):  
Ethylene Glycol ◽  
Silver Nitrate ◽  
Induction Period ◽  
Glass Forming Ability ◽  
Transformation Theory ◽  
Concentration Region ◽  
Glass Forming ◽  
Temperature Transformation ◽  
Silver Nitrate Solutions ◽  
Nitrate Solutions

The dependence of the induction period of crystallization on supercooling was examined for the silver nitrate-ethylene glycol system over the concentration region of silver nitrate lome fraction of 0 to 0.12. Addition of AgNO3 to ethylene glycol was found to increase considerably the critical induction period of crystallization, although to a lesser extent than Ca(NO3)2, CaCl2, ZnCl2, LiCl and LiNO3 do. The effect of these salts on the critical induction period of crystallization in dimethylsulfoxide, dimethylformamide, dimethylacetamide and methanol was compared in terms of the solvent-rich composition limit of the glass-forming ability. By using the TTT(Time-Temperature-Transformation) theory, it has been deduced that the effect of the salts on the critical induction period of crystallization of ethylene glycol is probably due to the different dependences of viscosity on their concentration in ethylene glyco in the supercooling region.

scholarly journals Functional Attributes of Myco-Synthesized Silver Nanoparticles from Endophytic Fungi: A New Implication in Biomedical Applications

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 473
Author(s):  
Prabu Kumar Seetharaman ◽  
Rajkuberan Chandrasekaran ◽  
Rajiv Periakaruppan ◽  
Sathishkumar Gnanasekar ◽  
Sivaramakrishnan Sivaperumal ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Culture Filtrate ◽  
Morphological Changes ◽  
Xrd Analysis ◽  
Penicillium Oxalicum ◽  
Breast Cancer Cell Lines ◽  
Face Centered Cubic ◽  
Tem Analysis ◽  
X Ray ◽  
Average Size

To develop a benign nanomaterial from biogenic sources, we have attempted to formulate and fabricate silver nanoparticles synthesized from the culture filtrate of an endophytic fungus Penicillium oxalicum strain LA-1 (PoAgNPs). The synthesized PoAgNPs were exclusively characterized through UV–vis absorption spectroscopy, Fourier Transform Infra-Red spectroscopy (FT-IR), X-ray powder diffraction (XRD), and Transmission Electron Microscopy (TEM) with energy dispersive X-ray spectroscopy (EDX). The synthesized nanoparticles showed strong absorbance around 430 nm with surface plasmon resonance (SPR) and exhibited a face-centered cubic crystalline nature in XRD analysis. Proteins presented in the culture filtrate acted as reducing, capping, and stabilization agents to form PoAgNPs. TEM analysis revealed the generation of polydispersed spherical PoAgNPs with an average size of 52.26 nm. The PoAgNPs showed excellent antibacterial activity against bacterial pathogens. The PoAgNPs induced a dose-dependent cytotoxic activity against human adenocarcinoma breast cancer cell lines (MDA-MB-231), and apoptotic morphological changes were observed by dual staining. Additionally, PoAgNPs demonstrated better larvicidal activity against the larvae of Culex quinquefasciatus. Moreover, the hemolytic test indicated that the as-synthesized PoAgNPs are a safe and biocompatible nanomaterial with versatile bio-applications.

scholarly journals Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3790
Author(s):  
Pratama Jujur Wibawa ◽  
Muhammad Nur ◽  
Mukhammad Asy’ari ◽  
Wijanarka Wijanarka ◽  
Heru Susanto ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Activated Carbon ◽  
Aloe Vera ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
E Coli ◽  
Transmission Electron ◽  
Average Size ◽  
Green Synthesized Silver Nanoparticles

This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

2013 ◽  
Vol 14 (1-2) ◽  
pp. 49-60 ◽  
Author(s):  
Francesca Broggi ◽  
Jessica Ponti ◽  
Guido Giudetti ◽  
Fabio Franchini ◽  
Vicki Stone ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Transformation ◽  
Micronucleus Test ◽  
Chemical Properties ◽  
Neoplastic Transformation ◽  
Research Centre ◽  
Ag Nps ◽  
Joint Research ◽  
Ic50 Values ◽  
Average Size

AbstractSilver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnology-based products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

Antimicrobial and Cytotoxic Activity of Silver Nanoparticles Stabilized by Natural Biopolymer Arabinogalactan

2020 ◽  
Vol 19 (04) ◽  
pp. 1950029
Author(s):  
A. G. Demchenko ◽  
V. S. Sadykova ◽  
A. V. Lyundup ◽  
N. E. Sedyakina ◽  
T. I. Gromovykh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antifungal Activity ◽  
Cytotoxic Activity ◽  
Chemical Reduction ◽  
Antitumor Agent ◽  
Escherichia Coli Bacteria ◽  
Trichoderma Citrinoviride ◽  
Average Size ◽  
Antibacterial And Antifungal ◽  
Dose Dependent

Silver nanoparticles were synthesized by chemical reduction of silver nitrate using arabinogalactan polysaccharide as a reducing agent and a stabilizer. The average size of nanoparticles, obtained by analyzing TEM-images, was 10.8[Formula: see text]nm; zeta potential [Formula: see text][Formula: see text]mV. A study of the sol by electron diffraction showed that silver in the sample is in metallic form. The resulting preparation of silver nanoparticles showed both antibacterial and antifungal activity. A pronounced antibacterial activity of silver nanoparticles was demonstrated both in relation to conditionally pathogenic gram-positive (Bacillus subtilis and B. coagulans) and gram-negative (Escherichia coli) bacteria. Silver nanoparticles also possess antifungal activity against macromycete Fomitopsis sp., as well as two strains of micromycetes Trichoderma citrinoviride and Fusarium sporotrichioides. Using the methods of light and fluorescence microscopy, MTT-analysis and Real-time cell analysis, the cytotoxic activity of silver nanoparticles was investigated on HepG2 human hepatocellular carcinoma cells. It was demonstrated that nanoparticles cause a suppression of cell metabolic and proliferative activity, as well as dose-dependent induction of cell death (average relative EC[Formula: see text] value was [Formula: see text]g/ml). The preparation of silver nanoparticles stabilized by arabinogalactan can be used in medicine, as a potential antimicrobial and antitumor agent.

scholarly journals Green Synthesis and Catalytic Activity of Silver Nanoparticles Based on Piper chaba Stem Extracts

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1777 ◽  
Author(s):  
Md. Mahiuddin ◽  
Prianka Saha ◽  
Bungo Ochiai
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Ftir Spectroscopy ◽  
Visual Observation ◽  
Face Centered Cubic ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Face Centered ◽  
Average Size

A green synthesis of silver nanoparticles (AgNPs) was conducted using the stem extract of Piper chaba, which is a plant abundantly growing in South and Southeast Asia. The synthesis was carried out at different reaction conditions, i.e., reaction temperature, concentrations of the extract and silver nitrate, reaction time, and pH. The synthesized AgNPs were characterized by visual observation, ultraviolet–visible (UV-vis) spectroscopy, dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), x-ray diffraction (XRD), energy dispersive x-ray (EDX), and Fourier transform infrared (FTIR) spectroscopy. The characterization results revealed that AgNPs were uniformly dispersed and exhibited a moderate size distribution. They were mostly spherical crystals with face-centered cubic structures and an average size of 19 nm. The FTIR spectroscopy and DLS analysis indicated that the phytochemicals capping the surface of AgNPs stabilize the dispersion through anionic repulsion. The synthesized AgNPs effectively catalyzed the reduction of 4-nitrophenol (4-NP) and degradation of methylene blue (MB) in the presence of sodium borohydride.

scholarly journals Synthesis, Characterization, and Optimization of Green Silver Nanoparticles Using Neopestalotiopsis clavispora and Evaluation of Its Antibacterial, Antibiofilm, and Genotoxic Effects

2021 ◽  
Vol 5 (3) ◽  
pp. 109-122
Author(s):  
Tuğba Kahraman ◽  
Safiye Elif Korcan ◽  
Recep Liman ◽  
İbrahim Hakkı Ciğerci ◽  
Yaser Acikbas ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Nuclear Ribosomal Dna ◽  
Diffusion Method ◽  
Dependent Manner ◽  
Transmission Electron ◽  
Infrared Spectrophotometer ◽  
Average Size ◽  
Novel Applications ◽  
Dose Dependent

Abstract Silver nanoparticles (AgNPs) have been used in a variety of biomedical applications in the last two decades, including antimicrobial, anti-inflammatory, and anticancer treatments. The present study highlights the extracellular synthesis of silver nanoparticles AgNPs using Neopestalotiopsis clavispora MH244410.1 and its antibacterial, antibiofilm, and genotoxic properties. Locally isolated N. clavispora MH244410.1 was identified by Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. Optimization of synthesized AgNPs was performed by using various parameters (pH (2, 4, 7, 9 and 12), temperature (25, 35 and 45 °C), and substrate concentration (0.05, 0.1, 0.15, 0.2 and 0.25 mM)). After 72 hours of incubation in dark conditions, the best condition for the biosynthesis of AgNPs was determined as 0.25 mM metal concentration at pH 12 and 35 °C. Fungal synthesized AgNPs were characterized via spectroscopic and microscopic techniques such as Fouirer Transform Infrared Spectrophotometer (FTIR), UV-Visible Spectroscopy, and Transmission Electron Microscopy (TEM). The average size of the AgNPs was determined less than 60 nm using the TEM and Zetasizer measurement system (measured in purity water suspension). The characteristic peak of AgNPs was observed at ~414 nm from UV-Vis results. Antibacterial and genotoxic activity of synthesized AgNPs (0.1, 1, and 10 ppm) were also determined by using the agar well diffusion method and in vivo Somatic Mutation and Recombination Test (SMART) in Drosophila melanogaster. AgNPs exhibited potential antimicrobial activity against all the tested bacteria (Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa) except Escherichia coli in a dose-dependent manner. AgNPs did not induce genotoxicity in the Drosophila SMART assay. 79.33, 65.47, and 41.95% inhibition of biofilms formed by P. aeruginosa were observed at 10, 1, and 0.1 ppm of AgNPs, respectively. The overall results indicate that N. clavispora MH244410.1 is a good candidate for novel applications in biomedical research.

scholarly journals Synthesis of Silver Nanoparticles from Anagalis arvensis and Their Biomedical Applications

2018 ◽  
Author(s):  
Mohib Shah ◽  
Natasha Anwar ◽  
Samreen Saleem ◽  
Iqbal Munir ◽  
Niaz Ali Shah ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Reducing Power ◽  
Spherical Shape ◽  
Artemia Salina ◽  
Maximum Activity ◽  
Tem Analysis ◽  
Synthesis Of Nanoparticles ◽  
The Stability ◽  
Average Size ◽  
Biological Methods

Background. Nanotechnology is promising field for generating new applications. A green synthesis of nanoparticles through biological methods using plant extract have a reliable and ecofriendly approach to improve our global environment. Methods. Silver nanoparticles (AgNPs) were synthesized using aqueous extract of Anagalis arvensis L and silver nitrate and were physicochemically characterized. Results. The stability of AgNPs toward acidity, alkalinity, salinity and temperature showed that they remained stable at room temperature for more than two months. The SEM and TEM analysis of the AgNPs showed that they have a uniform spherical shape with an average size in the range of 40–78 nm. Further 1-Dibhenyl-2-Picrylhydrazl radical in Anagalis arvensis L.mediated AgNPs showed a maximum activity of 98% at concentration of 200μg/mL. Hydrogen peroxide scavenging assay in Anagalis arvensis L. mediated AgNPs showed a maximum activity of 85% at concentration of 200μg/mL. Reducing power of Anagalis arvensis L.Ag NPs exhibited a higher activity of 330 μg/mL at concentration of 200 μg/mL. These NPs have cytotoxic effects against brine shrimp (Artemia salina) nauplii with a value of 53% LD 178.04μg/mL. Conclusion. The AgNPs synthesized using Anagalis arvensis L. extract demonstrate a broad range of applications.

scholarly journals Silver Nanoparticles Formation by Jatropha integerrima and LC/MS-QTOF-Based Metabolite Profiling

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2400
Author(s):  
Afrah E. Mohammed ◽  
Lamya Ahmed Al-Keridis ◽  
Ishrat Rahman ◽  
Modhi O. Alotaibi ◽  
Rasha Saad Suliman ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Laser Scanning ◽  
Antibacterial Effect ◽  
Therapeutic Potential ◽  
Laser Scanning Microscopy ◽  
Ultrastructural Changes ◽  
Confocal Laser ◽  
Synthesis Methods ◽  
Tem Analysis ◽  
Average Size

The broad application of metal nanoparticles in different fields encourages scientists to find alternatives to conventional synthesis methods to reduce negative environmental impacts. Herein, we described a safe method for preparing silver nanoparticles (J-AgNPs) using Jatropha integerrima leaves extract as a reducing agent and further characterize its physiochemical and pharmacological properties to identify its therapeutic potential as a cytotoxic and antimicrobial agent. The biogenic synthesized J-AgNPs were physiochemically characterized by ultraviolet-visible spectroscopy, dynamic light scattering (DLS), transmission electron microscope (TEM), and energy-dispersive X-ray spectroscopy. HPLC-DAD, followed by LC/MS and the Fourier-transform infrared spectroscopy (FTIR), was applied to detect the biomolecules of J. integerrima involved in the fabrication of NPs. Furthermore, J-AgNPs and the ampicillin-nanocomposite conjugate were investigated for their potential antibacterial effects against four clinical isolates. Finally, cytotoxic effects were also investigated against cancer and normal cell lines, and their mechanism was assessed using TEM analysis and confocal laser scanning microscopy (LSM). Ag ions were reduced to spherical J-AgNPs, with a zeta potential of −34.7 mV as well as an average size of 91.2 and 22.8 nm as detected by DLS and TEM, respectively. HPLC GC/MC analysis identified five biomolecules, and FTIR suggested the presence of proteins besides polyphenolic molecules; together, these molecules could be responsible for the reduction and capping processes during NP formation. Additionally, J-AgNPs displayed a strong antibacterial effect, although the ampicillin conjugated form had a very weak antibacterial effect. Furthermore, the NPs caused a reduction in cell viability of all the treated cells by initiating ultrastructural changes and apoptosis, as identified by TEM and LSM analysis. Therefore, J-AgNPs can be formed using the leaf extract from the J. integerrima plant. Furthermore, J-AgNPs may serve as a candidate for further biochemical and pharmacological testing to identify its therapeutic value.

Sign in / Sign up

Export Citation Format

Share Document