scholarly journals The Effect of Papain Enzymes and Probiotic Addition on Feed Towards The Catfish Growth Performance, Feed Utilization Efficiency and Survival Rate in Biofloc Techniques

Author(s):  
Kumala Hidayatiningtyas ◽  
Siti Harnina Bintari ◽  
Retno Sri Iswari

The success of catfish farming (Clarias sp.) is influenced by feed and water quality. The low feed utilization results in high feed production costs. Alternative use of feed using the enzyme papain and probiotics, the papain enzyme is able to hydrolyze proteins into peptides to increase the utilization of protein in feed by the body. In addition, increased digestibility is done by the addition of probiotic bacteria such as Lactobacillus sp., Saccharomyses sp., Azotobacter sp., Streptomyces sp., Aspergillus sp., Trichoderma sp. into food that can increase feed digestibility and nutrient absorption. Water quality is one of the factors that caused a low survival rate of fish. Biofloc technology utilizes microbial activity that can convert organic waste intensively to form a flock that used by fish as a food source. This study aims to examine the effect of papain and probiotics enzyme addition in feed towards the growth, efficiency of feed utilization and survival rate of catfish using biofloc techniques. The research used a Completely Randomized Design (CRD) by five treatments using biofloc maintenance media and five treatments using non-biofloc, each repeated three times. This research used catfish with a density of 1 head /L kept for 4 weeks as a test animal, treatment P0 (test feed 0.0 gr / kg papain enzyme and 0 mL probiotics), P1 (test feed 0.25 gr / kg papain enzyme and 1 mL probiotic), P2 (Test feed 0.25 gr / kg papain enzyme and 2 mL probiotics), P3 (Test feed 0.5 gr / kg papain enzyme and 1 mL probiotic), P4 (Test feed 0.5 gr / kg of papain enzyme and 2 mL probiotics). The results showed that the addition of the papain and probiotics enzyme in feed with the best dosage was P2 with biofloc maintenance media that gave a real effect (P <0.05) on the efficiency of feed utilization and survival, but had no significant effect (P> 0.05) on growth of catfish (Clarias sp.). Water quality in the maintenance media is in a decent range for the maintenance of test fish.

2019 ◽  
Vol 20 (2) ◽  
pp. 103
Author(s):  
Rozi Rozi ◽  
Akhmad Taufiq Mukti ◽  
Syifania Hanifah Samara ◽  
Muhammad Browijoyo Santanumurti

Chitosan is a functional polysaccharide that is capable of inducing various biological activities. This research aims to examine the effect of chitosan on growth and blood profile of tilapia fish. The method used in this research was experimental methods with completely randomized design (RAL) consisting of 4 treatments and 3 times. 120 Tilapia fish in 5-7 cm in range size were reared in aquarium at density of 10 fish/aquarium. Feedwere given at the additional dose of 10 ppt, 50 ppt, and 100 ppt chitosan. The observed variables were growth rate, survival rate, fish blood profile, water quality, feed utilization efficien . The results showed that the best chitosan treatment was at a dose of 100 ppt which produced FCR of 3.48 ± 2.25, AGP: 0.13 ± 0.03, SGR: 0.85 ± 0.84 and EPP: 28.70 ± 16,54.


2020 ◽  
Vol 8 (2) ◽  
pp. 176-192
Author(s):  
Tiara Putri ◽  
Supono Supono ◽  
Berta Putri

The study aimed to determine how the growth and survival capabilities of vaname shrimp larvae (Litopenaeus vannamei) are affected by different feeding methods and thereby the most efficient method. The study was conducted at the PT. Citra Larva Cemerlang, South of Kalianda in Lampung Province. For the experiment Completely Randomized Design (CRD) was utilized with 3 feeding methods and each was replicated 3 times. The feeding methods tested were: fozen, liquid and powdered Artemia feed. The experiments were done with up to 5000 shrimps on Zoea 1 stadia per month, which were kept in rearing tanks for 17 days.The test parameters observed were absolute growth, specific growth rate, efficiency of feed utilization, survival rate and water quality. Based on the results of statistical analysis (Anova) feeding frozen Artemia gave the best results with absolute growth of 5,6 mg, daily growth rate of 0,33 mg/hari, efficiency of feed utilization of 0,27%, and survival rate of 72,9%. Water quality during maintenance temperatures obtained is 27,1-32oC, pH 7,73-8,26, dissolved oxygen 3,8-7,1 mg/l, salinity 30-34 ppt, ammonia 0,02-1,57 mg/l. Keywords: Litopenaeus vannamei, Artemia, growth, frozen, powder, liquid.


2021 ◽  
Vol 7 (2) ◽  
pp. 1-6
Author(s):  
Numisye Iske Mose ◽  
Jetti Treslah Saselah

Tanaman wori merupakan salah satu tanaman kelompok fabaceace yang tumbuh melimpah di Sangihe dan sangat potensial digunakan sebagai salah satu sumber pakan ikan. Kadar protein daun wori adalah 25,53% sehingga berpotensi menjadi protein nabati bagi ikan. Kendala utama yang ditemui ketika memanfaatkan daun tanaman wori adalah kandungan karbohidrat yang tinggi yaitu 48,51% sehingga dapat mempengaruhi proses pencernaan ikan, penyerapan nutrisi yang berujung pada rendahnya pertumbuhan ikan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tepung daun wori femerntasi terhadap laju pertumbuhan spesifik dan sintasan hidup ikan nila. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL). Perlakuan yang dilakukan terdiri dari tiga perlakuan dan tiga kali ulangan yaitu kadar tepung daun wori tanpa fermentasi A (kontrol) 10%, kemudian B tepung daun wori fermentasi (10%), dan C tepung daun wori fermentasi (20%). Laju pertumbuhan spesifik dan efisiensi pakan diuji menggunakan ANOVA sedangkan sintasan hidup akan dianalisis secara deskriptif. Hasil penelitian ini menunjukan bahwa penambahan tepung wori fermenasi dalam pakan dapat meningkatkan nilai laju pertumbuhan spesfik ikan nila dan efisiensi pemanfaatan pakan dengan nilai tertinggi pada perlakuan C yaitu SGR 1,94%/hari. Sementara itu, untuk semua perlakuan nilai sintasan hidup sebesar 100%.   The wori plant is one of the plants of the fabaceace group that grows abundantly in Sangihe and has potential to be used as a source of fish feed. The protein content of wori leaves is 25.53%, so it has the potential to become vegetable protein for fish. The main obstacle encountered when utilizing wori plant leaves is the high carbohydrate content of 48.51% so that it can affect the digestion process of fish, nutrient absorption which lead to low fish growth. The purpose of this study was to determine the effect of wori femerntation leaf meal on the specific growth rate and survival of tilapia. The experimental design used was a completely randomized design (CRD). The treatments consisted of three treatments and three replications, namely the content of fermented wori leaf powder, A (control) 10%, then B fermented wori leaf powder (10%), and C fermented wori leaf powder (20%). Specific growth rate and feed efficiency were tested using ANOVA, while survival rate was analyzed descriptively. The results of this study indicate that the addition of fermented wori powder in the feed can increase the value of the specific growth rate of tilapia and feed utilization efficiency with the highest value in treatment C, namely SGR 1.94% / day. Meanwhile, for all treatments the survival rate was 100%.


2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Retno Wijayanti ◽  
Muarif Muarif ◽  
Dudi Lesmana

This research was carried out on March to August, 2018, and the experiment on April to June, 2018 at the Fishery Laboratory, Djuanda University Bogor. The objective of research is to know and analyze the survival rate and feed conversion ratio of Giant Gouramy that cultured on biofloc media and different protein levels. The experimental design that used is completely randomized design   with 2 treatments   and 6 replications.  The treatments are  treatment A (feeding of protein level  of 17% + mollase addition  with C,/N  ratio 12) and treatment B (feeding of protein  level  of  30%  without  mollase)  Fish that used is Giant Gouramy fry with length 5-7 cm that maintained cylinder container of diameter 1 m and height 1 m. The results of research that the artificial feeding of protein level of 17% + mollase addition with C/N ratio 12 gives the survival rate which is better, but the feed conversion ratio is bad. The average of the highest survival rate of Giant Gouramy is 89% (treatment A) and the low feed conversion ratio is 1.6 (treatment B). Water  quality during the research are temperature  of  23,6-30,0 °C, dissolved oxygen of 6.4- 8.8 mg/l and  pH of 6,3-8,8.Key words: Giant Gouramy, biofloc, feed conversion ratio, protein level.


2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Ayu Herdianti Primashita ◽  
Boedi Setya Rahardja ◽  
Prayogo .

Catfish (Clarias sp.) is a freshwater fish that is widely cultivated because it is a leading commodity. Increased production of catfish resulted in extra area of cultivated land and water use. The technology suitable to be applied is aquaponic system. In addition to saving land and water use, Aquaponic is also improving business efficiency through the utilization of nutrients from food remains and fish metabolism. Water quality is an important factor for the success of cultivation. Maintenance catfish with the addition of probiotics in aquaponic system can be a solution for maintaining water quality, because it contains bacteria that can increase nitrite to nitrate changes that can be utilized for growth of plants and do not poison the fish. The purpose of this study was to determine the effect of different probiotics in the aquaponic system towards growth rate and survival rate of catfish. The method that used in this research is experimental with completely randomized design (four  treat and five repeated) are P0 (control), P1 (probiotic A), P2 (probiotic B) and P3 (probiotic C). Analysis of data processed using Analysis of Variance. If there are significant differences then continued Duncan's Multiple Range Test. The results showed that the giving of probiotics in the aquaponic system is significantly different (p <0.05). The final conclusion is that the addition of probiotics in  aquaponic system affect the growth rate and survival rate of catfish. The highest specific growth rate (0.025%/ day) and the highest survival rate (77.8%) contained in the P2 treatment. The lowest specific growth rate (0.019% /day) and the lowest survival rate (64.4%) contained in P0 treatment (control). Keywords : Aquaponic, Catfish, Probiotic, Growth Rate, Survival Rate, Clarias sp.


2020 ◽  
Vol 27 (2) ◽  
pp. 154
Author(s):  
Vivi Endar Herawati ◽  
Pinandoyo Pinandoyo ◽  
Seto Windarto ◽  
Putut Hariyadi ◽  
Johannes Hutabarat ◽  
...  

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17±4.58, FUE of 27.51±0.77, PER of 0.83±0.03%, and RGR of 2.34±0.10.


2017 ◽  
Vol 5 (1) ◽  
pp. 70-82 ◽  
Author(s):  
Annisa Siregar ◽  
Dade Jubaedah ◽  
Marini Wijayanti

ABSTRACT Fitoremediation is one of water quality management system by using water plant such as Hydrilla verticillata that is purposed to decrease organic materials. This research was conducted at Laboratorium Dasar Perikanan, Aquaculture Study Program, Faculty of Agriculture, Sriwijaya University,Indralaya on May – July 2016. The research based on a Completely Randomized Design with four treatments and three replications. The treatments were media without H. verticillata (P0), addition of H. verticillata as much as 85 ± 10 g (P1), 110 ± 10 g (P2) and 135 ± 10 g (P3). The parameters were water quality, survival rate and specific growth rate of catfish. The data were analyzed by regressions analysis, analysis of variance and least significant diference test. The result showed that the addition of H. verticillata could improved water quality. The treatment of addition ofH. verticillata as much as 135 ± 10 g (P3) has highest survival rate 95.49 %, spesific growth rate for length 1.53 %.day-1 and weight 5.25 %.day-1. Keywords: Catfish,  Hydrilla verticillata, Phytoremediation, Water Quality 


Author(s):  
Kiki Haetami ◽  
Walim Lili ◽  
Roffi Grandiosa ◽  
Freddy Aditya Destiyantara

This study aims to analyze the effect of red paprika flour in fish feed to increase the color intensity of the Comet fish juvenile. The research method used a Complete Randomized Design experiment consist of four treatments and three replicates. The treatments consisted of various levels of addition red pepper extract, namely treatment A (0%), B (3%), C (5%) and D (7%). The investigation includes color intensity of the fish. Survival rate and water quality parameters data were also taken. The color observation data were analyzed using the Kruskal-Wallis analysis, if there was a significant difference, the Z test was performed. The results of this study indicated that the addition of 5% red paprika extract to the feed was the best treatment with an increase in the color intensity level of comet fish at 4.71. While the comet fish Survival Rate (SR) data in all treatments were observed to be 100% and the water quality during the maintenance period was in the optimal range and met the standards. The average temperature value was 24.7-26.4ºC, DO 5.6-5.8 mg/L and pH 6.67-7.53.


2020 ◽  
Vol 23 (2) ◽  
pp. 265-274
Author(s):  
Diana Rachmawati ◽  
Johannes Hutabarat ◽  
Eko Nurcahya Dewi ◽  
Seto Windarto

Feed utilization efficiency is one of the problems that often faced by tiger shrimp (Penaeus monodon Fabricus) farmers, which is 40-60% of production costs used for feed. The efficiency of feed can be improved by the addition of the bromelains in the feed, which can hydrolyze proteins into simpler compounds to increase the digestibility of the feed protein. This study aims to examine the effect of bromelains in feed on protein digestibility (ADCp), feed utilization efficiency (EPP), and growth (RGR) of tiger shrimp. Tiger shrimp with an average weight of 2.8 ± 0.26 g / individual was obtained from the Brackish Water Aquaculture Development Centre (BBPBAP), Jepara. There were 6 treatments adding bromelains in the feed, A (0%), B (0.2%), C (0.4%), D (0.6%) and E (0.8%). Observation parameters, including ADCp, EPP, PER, RGR, SR, and water quality parameters. The results showed that the addition of the bromelains in feed increased ADCp, EPP, PER, RGR, but had no effect on the SR of tiger shrimp. Bromelains (0.4% / kg) of feed is the optimal dose of ADCp, EPP, and RGR, resulting in a maximum value of 82.59%, 88.17%, and 5.24% / day.   Permasalahan yang sering dihadapi pembudidaya udang windu (Penaeus monodon Fabricius) adalah efisiensi pemanfaatan pakan yang belum maksimal sehingga hampir 40-60% dari total biaya produksi untuk biaya pakan. Efisiensi pemanfaatan pakan dapat ditingkatkan dengan penambahan enzim bromelin dalam pakan yang mampu menghidrolisis protein menjadi senyawa yang lebih sederhana sehingga dapat meningkatkan daya cerna protein pakan. Penelitian ini bertujuan mengkaji pengaruh enzim bromelin dalam pakan terhadap kecernaan protein (ADCp), efisiensi pemanfaatan pakan (EPP) dan pertumbuhan (RGR) udang windu. Hewan uji berupa udang windu dengan bobot rata-rata 2,8±0,26 g/ekor diperoleh dari Balai Besar Perikanan Budidaya Air Payau (BBPBAP), Jepara sebagai tempat penelitian. Terdapat 6 perlakuan penambahan enzim bromelin dalam pakan yaitu A (0 %), B (0,2 %), C (0,4 %), D (0,6 %) dan E (0,8 %). Pengamatan parameter meliputi ADCp, EPP, PER, RGR, SR dan parameter kualitas air. Hasil penelitian menunjukkan bahwa penambahan enzim bromelin dalam pakan meningkatkan ADCp, EPP, PER, RGR, namun tidak berpengaruh pada SR udang windu. Enzim bromelin 0,4%/kg pakan merupakan dosis optimal pada ADCp, EPP dan RGR menghasilkan nilai maksimal sebesar 82,59%, 88,17% dan 5,24%/hari.


2019 ◽  
Vol 7 (3) ◽  
pp. 1
Author(s):  
Otchoumou Kraidy Athanase ◽  
Blé Mélécony Célestin ◽  
Saki Suomion Justin ◽  
Alla Yao Laurent

A 56-day feeding trial was performed to examine the effects of using Nymphaea lotus leaf meal (NLM) on growth performance, feed utilization, body composition, and survival rate of African catfish (Heterobranchus longifilis). Five treatment groups in triplicates with an average of the initial weight of 0.26±0.07g were fed diets incorporated with graded levels of NLM (0, 10, 20, 30 and 40%). The obtained results showed that higher final body weight (FBW), daily weight gain (DWG), and specific growth rate (SGR) were observed with fish fed the control diet (CD) and NLM at the level of 20% compared to those of fish fed other diets. Protein efficiency ratios (PER) were not altered with dietary treatment. In addition, feed conversion ratio (FCR) of fish groups fed up to 30% of NLM were not varied (p>0.05), meanwhile, daily feed intake of fish fed NLM diets exhibited lower values compared to those of fish fed CD. Final whole body proximate compositions of catfish showed no significant differences (P>0.05) except for the body total fat and ash contents. Fat contents of fish fed diets incorporated with NLM were significantly lower than those of fish fed the control diet (CD) (P<0.05). Meanwhile, ash contents of fish fed diets incorporated with high levels of NLM (30, 40%) were significantly lower than those of fish fed the control diet (CD) (P<0.05). Survival rates ranged between 72 - 80% and there were no differences among all groups (P > 0.05). Therefore, the results of the present study suggest that up to 20% of NLM protein can be incorporated in the diet of African catfish with no adverse effects on growth performance, feed utilization, and survival rate.


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