scholarly journals Immunological phenotypes of intolerance of dental materials

2016 ◽  
pp. 40-49
Author(s):  
I. Karpuk
Keyword(s):  
T Lymphocytes ◽  
Blood Serum ◽  
Metabolic Activity ◽  
Immune Status ◽  
Dental Materials ◽  
Control Group ◽  
Dental Alloys ◽  
T Helpers

The purpose of work was the research of the immune status and allocation of immunologic phenotypes at patients with the intolerance of stomatologic materials (ISM). Examination 91 patients aged from 46 up to 65 years, with complaints to ISM is conducted. As a result of a research of the T-cellular link of system of immunity at patients with an intolerance of stomatologic materials, but without the taped hypersensitivity to their components, taped the following patterns: statistically reliable (р<0,05) depression of the CD3+ levels of the T-general (47,9±3,7 at norm in control group – 66,2±4,5) and CD4+ of T-helpers (30,6±2,8 at norm of donors 38,2±3,2). It is noted reliable (р<0,05) depression of IRI (1,49±0,32 at norm of donors 1,76±0,36). At patients with complaints to an intolerance of stomatologic materials and the taped hypersensitivity, by results of application assays and reaction of allergenindutsirovanny damage of leucocytes the immune status was characterized reliable (р<0,05) by rising of quantity of the lymphocytes carrying CD25+ markers for 36,2% in comparison with those in control group (р<0,05). The augmentation of IRI (2,33±0,54) in comparison with control group (1,76±0,36) (р<0,05), generally at the expense of the increased CD4+ level of T-helpers and relative depression the maintenance of CD8+ of T lymphocytes to 18,3±3,6% is established (patients of control group have 21,7±3,09%). At that group depression of the IgA level (р<0,05) in blood serum to 1,36±0,12 g/l and in oral liqid to 0,042±0,0023 g/l is taped, and patients of control group have 2,57±0,06 g/l and 0,11±0,04 g/l respectively. The lowered activity of neutrocytes by results of spontaneous (8,4±3,5%) and induced (41,4±5,3%) is established to test NST at patients with ISM without hypersensitivity to dental alloys that authentically (р<0,05) differed with similar indicators of patients with ISM and the taped hypersensitivity to components of dental alloys. Indicators of the increased metabolic activity of neutrophils at patients with complaints to NSM and the taped hypersensitivity to dental alloys and their components defined in the NST-test correlated with: levels IgA of stomatic liquid (r=-0,55; р<0,05); quantity of dentoprosthetic units (r=0,72; р<0,05); terms of use of dentoprosthetic designs (r=0,47; р<0,05). The data obtained by us show two immunologic phenotypes of ISM: one with a hypersensitivity to them, the second with its absence. At both phenotypes signs of deficiency of local and general immunity which differed at these phenotypes are taped.

EFFECTІVENESS OF LASER THERAPY ІN COMPLEX TREATMENT OF HERPETIC STOMATITIS

2021 ◽  
Vol 74 (6) ◽  
pp. 1331-1335
Author(s):  
Yulia G. Kolenko ◽  
Tetiana O. Timokhina ◽  
Nina S. Khrol ◽  
Oksana V. Kononova ◽  
Olesya V. Lynovytska
Keyword(s):  
T Lymphocytes ◽  
Laser Therapy ◽  
Pain Syndrome ◽  
Control Group ◽  
Complex Treatment ◽  
Results Of Treatment ◽  
Clinical Observations ◽  
Immunological Tests ◽  
T Helpers ◽  
Positive Effect

The aim: The purpose of research is to improve the effectiveness of treatment by applying laser in complex treatment in patients with herpetic stomatitis. Materials and methods: Were examined 47 people (22 men and 25 women) with acute herpetic stomatitis. The patіents’ age was from 20 to 72 years. All patients underwent immunological tests such as (T-lymphocytes (CD3 +), T-helpers (CD4 +), cytotoxic T-lymphocytes (CD8 +), the content of immunoglobulins IgM, IgG, IgA and slgA in saliva to assess the іmmunological reactivity of the organism. Results: Clinical observations have shown that when using laser therapy, the results of treatment were better than in the control group. Positive dynamics was observed as early as 1-2 days after the start of laser use and was more pronounced when used in the early stages of the disease. The use of the laser promoted a more rapid suspension of new lesions, a decrease in pain syndrome, and a more rapid regression of herpetic lesions. We have found significant improvements in the arameters of cellular and humoral іmmunity in patients of the main group. Conclusions: Treatment of herpetic stomatitis is relevant and requires further improvement and the search for new methods. The use of a laser in the complex treatment of herpetic stomatitis has a positive effect on the course and іmmunological status of patients with herpetic stomatіtis. The manifestations of the disease healed faster, and the pain syndrome was less severe. Duration between іllness and treatment decreased on 19.7% and 26%. It is advіsable to include the method of treatment using a laser in the complex therapy of herpetic stomatitis.

scholarly journals Cellular immunity of rabbits incase of parasite association (Treponema cuniculi and Eimeria sp.)

2019 ◽  
Vol 21 (96) ◽  
pp. 8-13
Author(s):  
Y. V. Duda
Keyword(s):  
T Lymphocytes ◽  
B Lymphocytes ◽  
Cellular Immunity ◽  
B Lymphocyte ◽  
Absolute Number ◽  
Blood Parameters ◽  
Control Group ◽  
The Absolute ◽  
T Helpers ◽  
Experimental Group

Despite a huge number of studies, the uniqueness of antiparasitic immunity is so great that there is still insufficient knowledge of the factors contributing to the manifestation of the characteristics of immunity in mixed parasitic diseases of rabbits. Therefore, the question of the influence of the association of pathogens Treponema cuniculi and Eimeria sp. on indicators of cellular immunity of rabbits is relevant. The study was conducted on 59 male rabbits age 3–5 months of the Californian breed, selected by analogy. Animal were separated into two groups: healthy animals (control group) and sick animals (research group). Intensity of invasion was determined by the method of the Mac-Master. It has been established that the level of damage of rabbits by spirochetosis and eimeriosis was, on average, 1155.17 ± 184.87 and 6668.97 ± 284.16 pathogens in 1 g of feces. The count of T- and B-lymphocytes was determined by the method of spontaneous rosette-formation with sheep erythrocytes. Parasitizing the association of pathogens Treponema cuniculi and Eimeria sp. was revealed a high number of leukocytes (1.22 times, P < 0.001), which increased mainly due to lymphocytes, which were 1.45 times higher (P < 0.001), as well as neutrophilic metamyelocytes – 1.48 times (P < 0.05), eosinophils – 1.68 times (P < 0.001) and basophils – 1.57 times (P < 0.001) compared with similar blood parameters of healthy animals. In the blood of sick rabbits, the absolute number of T-lymphocytes (1.56 times, P < 0.001) and B-lymphocytes (3.02 times, P < 0.001) was significantly higher in comparison with a low number of O-lymphocytes (3.46 times, P < 0.001) compared with the control. This indicates the redistribution of lymphocytes to cells that carry T and B lymphocyte receptors on the plasma membrane. The absolute number of T-lymphocytes became high due to T-helpers, which in these animals were higher both in absolute (1.87 times, P < 0.001) and percentage (by 9.18%, P < 0.001) compared to control. Moreover, the percentage of T-suppressors in the blood of rabbits of the experimental group was significantly lower on 5.46% (P < 0.05) compared with the same blood count of healthy animals. Such a redistribution of the T-cell population in the peripheral blood of this group of rabbits led to an increase in the immunoregulatory index by 1.64 times (P < 0.01) than in healthy ones. High IRI and the number of T-active lymphocytes (by 28.23%, P < 0.05) in the blood of rabbits with parasitism of the association of pathogens Treponema cuniculi and Eimeria sp. indicate increased immune system tension.

scholarly journals T-lymphocytes phenotypic composition of peripheral blood in patients with Graves’ disease undergoing conservative therapy with thiamazole

2021 ◽  
Vol 67 (6) ◽  
pp. 39-49
Author(s):  
M. A. Dudina ◽  
S. A. Dogadin ◽  
A. A. Savchenko ◽  
V. D. Belenyuk
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Controlled Trial ◽  
Effective Control ◽  
Control Group ◽  
Direct Immunofluorescence ◽  
Graves Disease ◽  
Open Label ◽  
T Helpers

BACKGROUND: Effective control of autoimmune inflammation in Graves’ disease determines necessity to study the T helper (Th) and cytotoxic T-lymphocytes dysfunction, as well as the level of regulatory T-cells (Treg) activation in patients with Graves’ disease on thyrostatic medication, which will clarify the immunomodulatory effects of long-term thiamazole treatment serve as targets for more specific therapies.AIM: To study the phenotypic composition of T-lymphocytes in the peripheral blood of patients with Graves’ disease to assess the direction of immune response depending on thimazole-induced euthyroidism duration.MATERIALS AND METHODS: A single-center, cohort, continuous, open-label, controlled trial was conducted to assess the phenotypic composition of T-lymphocytes in peripheral blood in women with Graves’ disease on long-term thiamazole treatment. The phenotypic composition of T-lymphocytes was determined by flow cytometry using direct immunofluorescence with conjugated FITC monoclonal antibodies depending on the duration of thimazole-induced euthyroidism of long-term thiamazole treatment.RESULTS: The study included 135 women with Graves’ disease, mean age 43.09±12.81 years, 120 (88.91%) with a relapse of the disease and 15 (11.09%) with newly diagnosed hyperthyroidism. An increase of activated CD3+CD4+CD25+ was found in patients with Graves’ disease with a duration of thimazole-induced euthyroidism 5–8 months and 9–12 months, respectively, Me=0.94 (0.48–1.45), p=0.020) and Me=0.95 (0.41–1.80), p=0.025), in control group — Me=0.12 (0.03–0.68). Compared to the control an increase of CD4+CD25+CD127Low (Treg) was found in patients with a duration of thimazole-induced euthyroidism 5–8 and 9–12 months. The content of Treg in peripheral blood in Graves’ disease patients with a duration of thimazole-induced euthyroidism more than 12 months decreases, but remains elevated relative to the control.CONCLUSION: In patients with Graves’ disease with a duration of thimazole-induced euthyroidism 5–8 months and 9–12 months the level of Treg has been increased. The increase of activated Th (CD3+CD4+CD25+) persists independently of thimazole-induced euthyroidism. In patients with Graves’ disease with a duration of thimazole-induced euthyroidism for more than 12 months, there is a compensatory increase in regulatory T-lymphocyte, and the total number of T-helpers is restored to the control.

scholarly journals The effect of interferons-α and –γ and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions

2019 ◽  
pp. 53-57
Author(s):  
A. G. Shakhov ◽  
I. T. Shaposhnikov ◽  
Yu. N. Brigadirov ◽  
V. N. Kotsarev ◽  
V. N. Skorikov ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Functional State ◽  
Reproductive System ◽  
Immune Complexes ◽  
Immune Status ◽  
Lysozyme Activity ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Ecological Conditions ◽  
Positive Effect

The results of studying the effect of interferons alpha and gamma and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions are presented. The positive effect of the drugs, especially their combination, on erythropoiesis and the immune status of animals, which was manifested by an increase in the content of erythrocytes, hemoglobin, hematocrit, leukocytes, total immunoglobulins, serum bactericidal and lysozyme activity, absorptive function of phagocytes, relative amount of T lymphocytes, and a decrease in the level of circulating immune complexes was stated. An increase in the immune status of animals at the effect of immunocorrecting drugs was accompanied by a decrease in the pathology of calving and postpartum complications and favorably affected the functional state of their reproductive system. The cows which were introduced interferons and their combination with dimethyl dipyrazolyl selenide demonstrated a shorter period from calving to successful insemination, as well as the insemination index and fertility, compared with the animals of the control group, which were not introduced any drugs.

scholarly journals Sex differences of subpopulation composition of lymphocytes in the peripheral blood in experimental acute and chronic ulcerative colitis

2020 ◽  
Vol 22 (1) ◽  
pp. 157-164
Author(s):  
O. V. Makarova ◽  
E. A. Postovalova ◽  
Yu. Gao ◽  
M. T. Dobrynina
Keyword(s):  
Sex Differences ◽  
T Lymphocytes ◽  
B Lymphocytes ◽  
Relative Number ◽  
Absolute Number ◽  
Control Group ◽  
Chronic Colitis ◽  
Acute Colitis ◽  
The Absolute ◽  
T Helpers

We studied sex differences lymphocytes subpopulations of peripheral blood in adult C57Bl/6 mice during acute and chronic colitis, induced with 1% DSS. We measured subpopulations of lymphocytes with flow cytometry. We showed that in the control group the female mice had statistically significantly higher values of the relative number of regulatory and cytotoxic T lymphocytes comparing to the males. During acute colitis the females showed an increase in the relative number of Thelpers and a decrease of cytotoxic Tlymphocytes, which reflects the activation of immune response. The males had a decrease in the absolute number of leukocytes, lymphocytes and cytotoxic and regulatory T lymphocytes, probably because of an increase in migration of these cells to the inflammation locus and local lymph nodes. In chronic colitis the females had a decrease in the absolute number of leukocytes, lymphocytes, T helpers, cytotoxic T lymphocytes and B lymphocytes when comparing with acute colitis. During chronic colitis the males had a decrease in the absolute number of T helpers and B lymphocytes but an increase of regulatory T cells in comparison with the control group; in comparison with acute colitis the males with chronic colitis had higher relative and absolute number of regulatory T cells. The increase of T regulatory lymphocytes is due to an increase in their proliferation rate in the thymus and increase of their migration to the inflammatory locus – the colon. Future clinical studies may be based on these results, which show that the treatment of colitis, especially with immunotropic agents, must take sex differences into account.

scholarly journals Immune Status of Patients with Polytrauma During the Period of Acute Reaction and Early Clinical Manifestations of the Traumatic Disease

2004 ◽  
Vol 11 (2) ◽  
pp. 32-36
Author(s):  
V V Agadzhanyan ◽  
M M Mendelenko ◽  
M V Semenikhina ◽  
E F Bikbaeva ◽  
E V Ivlev ◽  
...  
Keyword(s):  
Immune Deficiency ◽  
T Lymphocytes ◽  
Cellular Immunity ◽  
Immune Status ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Acute Reaction ◽  
T Helpers

The dynamics of immunologic indices in the acute and early periods of the traumatic disease was analyzed in 30 patients (25-60 years) with polytrauma. Even during the period of early manifestations of the traumatic disease the signs of immune deficiency are already determined as a disturbance of T-lymphocytes differentiation, T-helpers in particular. The changes in the system of cellular immunity coincide by time with the occurrence of clinical signs of secondary posttraumatic immune deficiency, i.e. pneumonias, pleurities, purulent endobronchites. The obtained data are indicative of the fact that patients with polytrauma already require immunostimulation during the period of early manifestations of the traumatic disease.

scholarly journals THE LEVELS OF SOME CHEMOKINES IN THE BLOOD SERUM OF PATIENTS WITH EXACERBATION OF CHRONIC PURULENT RHINOSINUSITIS

2017 ◽  
pp. 30-33
Author(s):  
O. A. Nakonechnaya ◽  
A. I. Onischenko ◽  
T. V. Gorbach ◽  
A. S. Tkachenko ◽  
T. N. Chubukova
Keyword(s):  
T Lymphocytes ◽  
Blood Serum ◽  
Chronic Rhinosinusitis ◽  
Cytotoxic T Lymphocytes ◽  
Nasal Septum ◽  
Inflammatory Process ◽  
Control Group ◽  
Healthy Individuals ◽  
Septum Deviation ◽  
Nasal Septum Deviation

Aim: to study the levels of fractalkine and IL-8 in blood serum of patients with exacerbation of chronic purulent rhinosinusitis. Material and methods . Twenty patients suffering from chronic purulent rhinosinusitis were examined during the stage of exacerbation. The control group consisted of twenty conditionally healthy individuals with nasal septum deviation. The blood sampling of the examined patients was performed. The levels of fractalkine and IL-8 in the blood serum were determined by ELISA. Results. The exacerbation of purulent chronic rhinosinusitis leads to increased levels of both fractalkine and IL-8 in the blood serum of patients, which contributes to the involvement of neutrophils, monocytes, and cytotoxic T-lymphocytes into the inflammatory process. Chemokines IL-8 and fractalkine play an important role in the pathogenesis of chronic purulent rhinosinusitis, which suggests the reasonability of application of pharmacological preparations that block their functions. Conclusions. The carried out study revealed increased levels of both fractalkine and IL-8 in the blood serum of the patients with chronic purulent rhinosinusitis at the stage of exacerbation

scholarly journals Effect of laser therapy on the immune status of patients with chronic salpingoophoritis of chlamidial etiology

2021 ◽  
pp. 65-70
Author(s):  
M. B. Mirzoeva
Keyword(s):  
T Lymphocytes ◽  
Laser Therapy ◽  
Phagocytic Activity ◽  
Immune Status ◽  
Low Frequency ◽  
Pcr Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
T Suppressors ◽  
Traditional Therapy ◽  
T Helpers

Aim. Justify the use of low-frequency laser radiation (LFLR) treating chronic salpingoophoritis of chlamydial etiology, taking into account the assessment of the immune status.Material and methods. The study included 110 women, of which 80 women had chronic salpingo-oophoritis of chlamydial etiology, and 30 were practically healthy. In addition to conventional methods, patients underwent examinations like immunological analysis, ultrasound examination of the pelvic organs, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) analysis.Results and discussion. When comparing changes in the immune status in patients after LFLR and who underwent traditional therapy in the group of patients with sexually transmitted infections (STIs), there was a slight increase in the number of T-lymphocytes and T-helpers, as well as a decrease in the number of T-suppressors. Also, in these patients, there is a slight decrease in the number of IgA and IgM against the background of an increase in IgG and an increase in the phagocytic activity of neutrophils. In patients with traditional therapy, the above-described changes persisted, while an aggravation of the disturbed T-cell balance was observed.Conclusions. Thus, according to the results of a comparative analysis of the obtained data on changes in the immune status in patients with chronic salpingo-oophoritis after the use of laser therapy, a statistically significant increase in the number of T-lymphocytes, an increase in subpopulations including T-helpers, a decrease in the number of T-suppressors, an increase in immunoglobulin G and an increase in phagocytic activity of neutrophils was observed.

scholarly journals The Dynamics of Immunological Indices in Patients with Posttraumatic Osteomyelitis and Trophic Disoders of the Shin Tissues

2020 ◽  
pp. 21-27
Author(s):  
N.O. Dekhtiarenko ◽  
M.P. Hrytsai ◽  
V.M. Tsokalo
Keyword(s):  
T Lymphocytes ◽  
Immune Status ◽  
Pathological Process ◽  
Statistical Research ◽  
Surgical Interventions ◽  
Posttraumatic Osteomyelitis ◽  
Humoral Immune ◽  
System A ◽  
Humoral Immune System ◽  
T Helpers

Summary. The article deals with the study of immune status of patients with osteomyelitis and trophic disorders of the shin tissues. Objective: to evaluate the immune status of patients with shin-bone osteomyelitis and distrophic processes, and to study dynamics of immunological indices after surgical interventions (clearing and reconstructive restoring surgeries). Materials and Methods. The immune status of 52 patients with posttraumatic osteomyelitis and trophic disorders of the shin tissues was studied. Clinical, immunological, and statistical research methods were used. Results. The study revealed changes in immune defence and in trophic processes in tissues (bones, skin, and muscles) of the damaged extremity. Thus, the presence of changes in the immune status was established: a decrease in the number of T-lymphocytes, T-helpers, cytotoxic T-lymphocytes, as well as changes in the humoral immune system – a decrease in immunoglobulins M and G levels and an increace in the level of circulating immune complexes. Conclusions. Changes in systemic immunity and local trophic disorders revealed in patients mutually aggravate the course of the pathological process and complicate its elimination.

scholarly journals Populational composition of CD4+5RA/ CD4+5RO positive t lymphocytes and cytokine profile in children with allergic respiratory diseases

2021 ◽  
Vol 23 (1) ◽  
pp. 149-156
Author(s):  
A. I. Turyanskaya ◽  
N. G. Plekhova ◽  
V. A. Sabynych ◽  
E. V. Procekova
Keyword(s):  
T Lymphocytes ◽  
Bronchial Asthma ◽  
Peripheral Blood ◽  
Respiratory Diseases ◽  
Allergic Inflammation ◽  
Allergic Diseases ◽  
Control Group ◽  
Healthy Children ◽  
Allergic Disorders ◽  
T Helpers

The changing states of T cell populations responsible for the chronic course of allergic inflammation and diseases, including allergic bronchial asthma, are not yet sufficiently characterized. The aim of this study was to detect phenotypic changes in the CD45RA/CD45RO positive T lymphocytes and the level of regulatory cytokines (TNFα, IFNγ, IL-4, IL-6, IL-8, IL-10, IL-13, IL-17А, IL-17F) in allergic respiratory diseases (ARD) in children. In blood of 90 children aged 3-11 (60 children with ARD and 30 healthy peers) were studied of the immune cellular populations and cytokine indices. The levels of IL-4, IL-8, IL-10, IL-13, IL-17A and IL-17F in blood serum of children with bronchial asthma and allergic rhinitis differed from appropriate indices in control group (p = 0.001). The quantity of CD3+CD8+CD45RACD45RO+ cells, T helpers (p < 0.05) and Th effectors simultaneously expressing both isoforms of the CD45RA+ and CD45RO receptor in peripheral blood of children with ARD significantly exceeded those in control group (p < 0.001). In healthy children, Th17 population (CD3+CD4+CD196 lymphocytes) comprised 9.49±1.6% of CD3+CD4+ of cells, the number of such lymphocytes was significantly increased to 14.5±0.77 in children with allergic diseases (p < 0.001). Absolute numbers of Th17+ cells were 93.0±9.30 and 127,0±72.0 cells/µl respectively (p = 0.002). Indicators of CD4CD45RO positive memory cells in children with ARD was determined as significantly lower (p < 0.001), whereas quantity of CD3+CD19+ proved to be higher (p < 0.05) than in healthy peers. Absolute counts of these cells did not differ between the groups. The number of CD8+CD45RO+T lymphocytes was significantly higher in children with allergic diseases (p < 0.025). This research shows that the quantitative ratio of CD3+CD8+CD45RA+ and CD3+CD8+CD45RO+ populations of T cells, and increased levels of cytokines, synthesizable via Th2 and Th17, in peripheral blood may be helpful for understanding genesis of allergic respiratory diseases, and extends our knowledge on immune mechanisms of allergic disorders for individualization of therapeutic programs.

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