scholarly journals Effect of laser therapy on the immune status of patients with chronic salpingoophoritis of chlamidial etiology

2021 ◽  
pp. 65-70
Author(s):  
M. B. Mirzoeva
Keyword(s):  
T Lymphocytes ◽  
Laser Therapy ◽  
Phagocytic Activity ◽  
Immune Status ◽  
Low Frequency ◽  
Pcr Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
T Suppressors ◽  
Traditional Therapy ◽  
T Helpers

Aim. Justify the use of low-frequency laser radiation (LFLR) treating chronic salpingoophoritis of chlamydial etiology, taking into account the assessment of the immune status.Material and methods. The study included 110 women, of which 80 women had chronic salpingo-oophoritis of chlamydial etiology, and 30 were practically healthy. In addition to conventional methods, patients underwent examinations like immunological analysis, ultrasound examination of the pelvic organs, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) analysis.Results and discussion. When comparing changes in the immune status in patients after LFLR and who underwent traditional therapy in the group of patients with sexually transmitted infections (STIs), there was a slight increase in the number of T-lymphocytes and T-helpers, as well as a decrease in the number of T-suppressors. Also, in these patients, there is a slight decrease in the number of IgA and IgM against the background of an increase in IgG and an increase in the phagocytic activity of neutrophils. In patients with traditional therapy, the above-described changes persisted, while an aggravation of the disturbed T-cell balance was observed.Conclusions. Thus, according to the results of a comparative analysis of the obtained data on changes in the immune status in patients with chronic salpingo-oophoritis after the use of laser therapy, a statistically significant increase in the number of T-lymphocytes, an increase in subpopulations including T-helpers, a decrease in the number of T-suppressors, an increase in immunoglobulin G and an increase in phagocytic activity of neutrophils was observed.

EFFECTІVENESS OF LASER THERAPY ІN COMPLEX TREATMENT OF HERPETIC STOMATITIS

2021 ◽  
Vol 74 (6) ◽  
pp. 1331-1335
Author(s):  
Yulia G. Kolenko ◽  
Tetiana O. Timokhina ◽  
Nina S. Khrol ◽  
Oksana V. Kononova ◽  
Olesya V. Lynovytska
Keyword(s):  
T Lymphocytes ◽  
Laser Therapy ◽  
Pain Syndrome ◽  
Control Group ◽  
Complex Treatment ◽  
Results Of Treatment ◽  
Clinical Observations ◽  
Immunological Tests ◽  
T Helpers ◽  
Positive Effect

The aim: The purpose of research is to improve the effectiveness of treatment by applying laser in complex treatment in patients with herpetic stomatitis. Materials and methods: Were examined 47 people (22 men and 25 women) with acute herpetic stomatitis. The patіents’ age was from 20 to 72 years. All patients underwent immunological tests such as (T-lymphocytes (CD3 +), T-helpers (CD4 +), cytotoxic T-lymphocytes (CD8 +), the content of immunoglobulins IgM, IgG, IgA and slgA in saliva to assess the іmmunological reactivity of the organism. Results: Clinical observations have shown that when using laser therapy, the results of treatment were better than in the control group. Positive dynamics was observed as early as 1-2 days after the start of laser use and was more pronounced when used in the early stages of the disease. The use of the laser promoted a more rapid suspension of new lesions, a decrease in pain syndrome, and a more rapid regression of herpetic lesions. We have found significant improvements in the arameters of cellular and humoral іmmunity in patients of the main group. Conclusions: Treatment of herpetic stomatitis is relevant and requires further improvement and the search for new methods. The use of a laser in the complex treatment of herpetic stomatitis has a positive effect on the course and іmmunological status of patients with herpetic stomatіtis. The manifestations of the disease healed faster, and the pain syndrome was less severe. Duration between іllness and treatment decreased on 19.7% and 26%. It is advіsable to include the method of treatment using a laser in the complex therapy of herpetic stomatitis.

scholarly journals Immunological phenotypes of intolerance of dental materials

2016 ◽  
pp. 40-49
Author(s):  
I. Karpuk
Keyword(s):  
T Lymphocytes ◽  
Blood Serum ◽  
Metabolic Activity ◽  
Immune Status ◽  
Dental Materials ◽  
Control Group ◽  
Dental Alloys ◽  
T Helpers

The purpose of work was the research of the immune status and allocation of immunologic phenotypes at patients with the intolerance of stomatologic materials (ISM). Examination 91 patients aged from 46 up to 65 years, with complaints to ISM is conducted. As a result of a research of the T-cellular link of system of immunity at patients with an intolerance of stomatologic materials, but without the taped hypersensitivity to their components, taped the following patterns: statistically reliable (р<0,05) depression of the CD3+ levels of the T-general (47,9±3,7 at norm in control group – 66,2±4,5) and CD4+ of T-helpers (30,6±2,8 at norm of donors 38,2±3,2). It is noted reliable (р<0,05) depression of IRI (1,49±0,32 at norm of donors 1,76±0,36). At patients with complaints to an intolerance of stomatologic materials and the taped hypersensitivity, by results of application assays and reaction of allergenindutsirovanny damage of leucocytes the immune status was characterized reliable (р<0,05) by rising of quantity of the lymphocytes carrying CD25+ markers for 36,2% in comparison with those in control group (р<0,05). The augmentation of IRI (2,33±0,54) in comparison with control group (1,76±0,36) (р<0,05), generally at the expense of the increased CD4+ level of T-helpers and relative depression the maintenance of CD8+ of T lymphocytes to 18,3±3,6% is established (patients of control group have 21,7±3,09%). At that group depression of the IgA level (р<0,05) in blood serum to 1,36±0,12 g/l and in oral liqid to 0,042±0,0023 g/l is taped, and patients of control group have 2,57±0,06 g/l and 0,11±0,04 g/l respectively. The lowered activity of neutrocytes by results of spontaneous (8,4±3,5%) and induced (41,4±5,3%) is established to test NST at patients with ISM without hypersensitivity to dental alloys that authentically (р<0,05) differed with similar indicators of patients with ISM and the taped hypersensitivity to components of dental alloys. Indicators of the increased metabolic activity of neutrophils at patients with complaints to NSM and the taped hypersensitivity to dental alloys and their components defined in the NST-test correlated with: levels IgA of stomatic liquid (r=-0,55; р<0,05); quantity of dentoprosthetic units (r=0,72; р<0,05); terms of use of dentoprosthetic designs (r=0,47; р<0,05). The data obtained by us show two immunologic phenotypes of ISM: one with a hypersensitivity to them, the second with its absence. At both phenotypes signs of deficiency of local and general immunity which differed at these phenotypes are taped.

scholarly journals Immune Status of Patients with Polytrauma During the Period of Acute Reaction and Early Clinical Manifestations of the Traumatic Disease

2004 ◽  
Vol 11 (2) ◽  
pp. 32-36
Author(s):  
V V Agadzhanyan ◽  
M M Mendelenko ◽  
M V Semenikhina ◽  
E F Bikbaeva ◽  
E V Ivlev ◽  
...  
Keyword(s):  
Immune Deficiency ◽  
T Lymphocytes ◽  
Cellular Immunity ◽  
Immune Status ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Acute Reaction ◽  
T Helpers

The dynamics of immunologic indices in the acute and early periods of the traumatic disease was analyzed in 30 patients (25-60 years) with polytrauma. Even during the period of early manifestations of the traumatic disease the signs of immune deficiency are already determined as a disturbance of T-lymphocytes differentiation, T-helpers in particular. The changes in the system of cellular immunity coincide by time with the occurrence of clinical signs of secondary posttraumatic immune deficiency, i.e. pneumonias, pleurities, purulent endobronchites. The obtained data are indicative of the fact that patients with polytrauma already require immunostimulation during the period of early manifestations of the traumatic disease.

scholarly journals The Dynamics of Immunological Indices in Patients with Posttraumatic Osteomyelitis and Trophic Disoders of the Shin Tissues

2020 ◽  
pp. 21-27
Author(s):  
N.O. Dekhtiarenko ◽  
M.P. Hrytsai ◽  
V.M. Tsokalo
Keyword(s):  
T Lymphocytes ◽  
Immune Status ◽  
Pathological Process ◽  
Statistical Research ◽  
Surgical Interventions ◽  
Posttraumatic Osteomyelitis ◽  
Humoral Immune ◽  
System A ◽  
Humoral Immune System ◽  
T Helpers

Summary. The article deals with the study of immune status of patients with osteomyelitis and trophic disorders of the shin tissues. Objective: to evaluate the immune status of patients with shin-bone osteomyelitis and distrophic processes, and to study dynamics of immunological indices after surgical interventions (clearing and reconstructive restoring surgeries). Materials and Methods. The immune status of 52 patients with posttraumatic osteomyelitis and trophic disorders of the shin tissues was studied. Clinical, immunological, and statistical research methods were used. Results. The study revealed changes in immune defence and in trophic processes in tissues (bones, skin, and muscles) of the damaged extremity. Thus, the presence of changes in the immune status was established: a decrease in the number of T-lymphocytes, T-helpers, cytotoxic T-lymphocytes, as well as changes in the humoral immune system – a decrease in immunoglobulins M and G levels and an increace in the level of circulating immune complexes. Conclusions. Changes in systemic immunity and local trophic disorders revealed in patients mutually aggravate the course of the pathological process and complicate its elimination.

scholarly journals Yiqi Wenyang Fang ameliorates allergic rhinitis through inhibiting inflammatory response and promoting the expression of Foxp3

2016 ◽  
Vol 29 (4) ◽  
pp. 696-706 ◽  
Author(s):  
Jun Shi ◽  
Yu Liu ◽  
Shihai Yan ◽  
Daonan Yan
Keyword(s):  
Allergic Rhinitis ◽  
Inflammatory Response ◽  
Transforming Growth Factor ◽  
Treg Cells ◽  
Pcr Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Dose ◽  
Sprague Dawley ◽  
Model Control ◽  
Tgf Β1

Allergic rhinitis (AR) is an inflammatory disease with a hypersensitivity response to environmental stimulus. The aim of this study was to evaluate the effect of Yiqi Wenyang Fang (YWF) on AR and investigate the underlying mechanism. A total of 48 female Sprague-Dawley rats were randomly divided into six groups (normal control, model control, YWF at low dose, YWF at median dose, YWF at high dose, and loratadine). Rats were injected with antigen for sensitization. Then, rats in the YWF groups were treated with different dose of YWF for 28 days. Loratadine was used as a positive control. Number of sneezes, degree of runny nose, nasal rubbing movements, and tissue damage were scored. The protein and mRNA expression of Foxp3 were determined by western blot and real time-PCR analysis, respectively. Flow cytometry was used to detect the number of CD4+CD25+Foxp3+ Treg cells. The content of interleukin (IL)-10, transforming growth factor β1 (TGF-β1), IL-13, and IL-4 in the serum were detected by enzyme-linked immunosorbent assay (ELISA). Scores of symptoms were significantly reduced and nasal mucosa damage was alleviated after YWF administration. YWF increased the expression of Foxp3, IL-10, TGF-β1, and number of CD4+CD25+Foxp3+ Treg cells which were reduced by antigen injection. The expression levels of IL-13 and IL-4 were increased after antigen administration while decreased after YWF treatment. YWF may ameliorate AR through inhibiting inflammatory response and promoting Foxp3 expression.

Specific features of T- and NK-cellular immunity in chronic lymphocytic leukemia

2021 ◽  
Vol 66 (6) ◽  
pp. 345-352
Author(s):  
Evgeniy Vladimirovich Pochtar ◽  
S. A. Lugovskaya ◽  
E. V. Naumova ◽  
E. A. Dmitrieva ◽  
A. I. Kostin ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Chronic Lymphocytic Leukemia ◽  
T Lymphocytes ◽  
Nk Cells ◽  
Lymphocytic Leukemia ◽  
Positive Trend ◽  
Functional Changes ◽  
Activated T Lymphocytes ◽  
T Helpers

Profound immunological dysfunction is the key factor determining the development of infectious complications in chronic lymphocytic leukemia (CLL). The aim of this work is to assess the features of the subpopulation composition of T-lymphocytes (T-helpers (Th), cytotoxic T-lymphocytes (Tcyt), T regulatory cells (Treg), T-NK cells, naive Th, Th-memory, activated T-lymphocytes, TCRγδ cells) and NK cells in peripheral blood of patients with newly diagnosed chronic lymphocytic leukemia (CLL) and receiving ibrutinib therapy. Hematological and immunophenotypic studies have been performed in 30 patients with previously untreated CLL, 122 patients on ibrutinib therapy and 20 healthy donors. The subpopulation composition of T-lymphocytes (Th, Tcyt, Treg, T-NK, naive T-helpers, memory T-helpers, TCRγδ cells, activated T-lymphocytes) and NK cells has been assessed on flow cytometer (FACSCanto II (BD)) using the following panel of monoclonal antibodies: CD45, CD19, CD3, CD4, CD5, CD8, TCRγδ, CD127, CD16, CD56, CD57 CD45RA, CD45R0, HLA-DR, CD25. Compared to controls all CLL samples were found to have higher the absolute number of T-lymphocytes, NK cells and their subpopulations, T-helpers (especially of memory T-cells), cytotoxic T-cells, regulatory T-cells, TCRγδ T-cells, activated T-lymphocytes, increased cytotoxic potential of NK cells in previously untreated CLL patients. Patients who received ibrutinib therapy have registered a positive trend towards recovery of the subpopulation composition of T-lymphocytes and NK-cells. CLL patients have been found to have quantitative and functional changes in the subpopulations of T-lymphocytes and NK cells, indicating dysregulation of the immune response, and a high risk of developing infections. Monitoring of immunological parameters for ibrutinib therapy make possible to estimate impact of ibrutinib on the adaptive anti-CLL immune response.

scholarly journals CSF-1-Induced DC-SIGN+ Macrophages Work In The Ovarian Endometriosis

2021 ◽  
Author(s):  
Xiaocui Li ◽  
Wei Hong ◽  
Yunlang Cai ◽  
Zhenzhen Zheng ◽  
Min An
Keyword(s):  
Peripheral Blood ◽  
Peritoneal Fluid ◽  
Immune Status ◽  
Biological Activities ◽  
Peripheral Blood Lymphocytes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Predominant Role ◽  
Blood Lymphocytes ◽  
Suppressive Phenotype

Abstract BackgroundCSF-1 was found to be accumulated in the lesions and peritoneal fluid of endometriosis patients, and CSF-1 induced THP-1-derived macrophages to polarize toward a suppressive phenotype. Researchers found that macrophages were the predominant cells in the peritoneal fluid (PF) of endometriosis patients, and the primary consensus is that the immune status in the PF of endometriosis patients exhibits a depressed state. Does the cytokine CSF-1 induce monocytes to differentiate into macrophages with a DC-SIGN+ suppressive phenotype in endometriosis?MethodsThe level of CSF-1 in control endometrium (N=11), eutopic endometrium (N=17), and ectopic (N=39) endometrium of endometriosis patients was evaluated by real-time polymerase chain reaction and in the PF of control (N=25) and endometriosis (N=35) patients by enzyme-linked immunosorbent assay. CSF-1 was examined by a MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel in an in vivo study. DC-SIGN+ suppressive macrophages were detected by immunohistochemical staining of tissues and flow cytometric analysis of the PF of control (N=25) and endometriosis (N=35) patients. The phenotypes and biological activities of the resulting macrophages derived from THP-1 cells induced by CSF-1 were compared by an in vitro coculture system with peripheral blood lymphocytes from normal subjects.Results In this study, we found the proportion of DC-SIGN+ suppressive macrophages was larger in the abdominal immune microenvironment of endometriosis patients. CSF-1 was primarily secreted from the ectopic lesions and peritoneum of mice with endometriosis. And, CSF-1 induced the polarization of macrophages toward a DC-SIGN+ suppressive phenotype; this effect was abolished by the addition of anti-CSF-1R. CSF-1 induced DC-SIGN+ macrophages, leading to a depressed status of peripheral blood lymphocytes, including a high percentage of Treg cells and a low percentage of CD8+ T cells. Similarly, blockade with anti-CSF-1R abrogated this biological effect. This is the first study on the predominant role of DC-SIGN+ suppressive macrophages in the depressed immune status of endometriosis patients.Conclusions This is the first study on the predominant role of DC-SIGN+ suppressive macrophages in the depressed immune status of endometriosis patients. Further study of the mechanism and biological activities of CSF-1-induced DC-SIGN+ suppressive macrophages will enhance our understanding of the physiology of endometriosis and indicate new directions for further study.

INFLUENCE OF INTEGRATED APPLICATION OF LASER THERAPY AND LOW-FREQUENCY ELECTROSTATIC FIELD ON BIOCHEMICAL INDICATORS OF ORAL FLUID IN PATIENTS WITH POSTPROTHETIC INFLAMMATORY COMPLICATIONS DURING DENTAL IMPLANTATION

2021 ◽  
pp. 8-17
Author(s):  
Варвара Ильинична Лившиц ◽  
Сергей Николаевич Нагорнев ◽  
Валерий Константинович Фролков ◽  
Галина Анатольевна Пузырева
Keyword(s):  
Lipid Peroxidation ◽  
Laser Therapy ◽  
Oral Fluid ◽  
Dynamic Balance ◽  
Low Frequency ◽  
Clinical State ◽  
Antioxidant Systems ◽  
Physical Factors ◽  
Dental Implantation ◽  
Close Relationship

В статье представлен анализ процессов липопероксидации в ротовой жидкости у пациентов с дентальными периимплантитами при курсовом применении низкоинтенсивной инфракрасной лазеротерапии и низкочастотной электростатической терапии. Полученные результаты позволяют заключить, что развитие воспалительных осложнений, возникающих у пациентов с ортопедическими конструкциями на дентальных имплантатах, характеризуются развитием окислительного стресса, нарушающим динамическое равновесие между про- и антиоксидантными системами. Применение физиотерапевтических факторов (лазера и низкочастотной электротерапии) оказывает выраженное антиоксидантное действие, реализуемое на основе потенцирующего механизма взаимодействия физических факторов и направленное на повышение резервной мощности защитных факторов ротовой жидкости и поддержание окислительного гомеостаза в условиях физиологического равновесия. Установлено, что позитивные сдвиги параметров липопероксидации тесно коррелируют с индексными показателями клинического состояния пациентов, что убедительно свидетельствует не только о патогенетической значимости процессов ПОЛ в развитии постпротетических воспалительных осложнений при дентальной имплантации, но и важности антиоксидантных потенций проводимых лечебных мероприятий в достижении клинической эффективности. Тесная взаимосвязь коэффициента стабильности дентального имплантата с продуктами ПОЛ характеризует собой деструктивное участие липоперекисных процессов в формировании морфологической основы остеоинтеграции имплантатов, а также позволяет рассматривать параметры перекисного метаболизма и значения коэффициента антиоксидантной защиты в качестве информативных индикаторов мониторинга и прогноза устойчивости внутрикостных дентальных конструкций The article presents an analysis of lipid peroxidation processes in the oral fluid in patients with dental peri-implantitis during the course of low-intensity infrared laser therapy and low-frequency electrostatic therapy. The results obtained allow us to conclude that the development of inflammatory complications arising in patients with orthopedic constructions on dental implants are characterized by the development of oxidative stress, which disrupts the dynamic balance between pro- and antioxidant systems. The use of physiotherapeutic factors (laser and low-frequency electrotherapy) has a pronounced antioxidant effect, realized on the basis of a potentiating mechanism of interaction of physical factors and aimed at increasing the reserve power of the protective factors of the oral fluid and maintaining oxidative homeostasis in conditions of physiological equilibrium. It has been established that positive shifts in lipid peroxidation parameters closely correlate with index indicators of the clinical state of patients, which convincingly indicates not only the pathogenetic significance of LPO processes in the development of postprotective inflammatory complications during dental implantation, but also the importance of antioxidant potencies of the therapeutic measures in achieving clinical efficacy. The close relationship of the coefficient of stability of a dental implant with LPO products characterizes the destructive participation of lipoperoxide processes in the formation of the morphological basis of osseointegration of implants, and also allows us to consider the parameters of peroxide metabolism and the value of the coefficient of antioxidant protection as informative indicators for monitoring and predicting the stability of intraosseous dental structures

Physiotherapy Complex for the Treatment of Children with Somatoform Dysfunction, Often Suffering from Recurrent Infection

2020 ◽  
Vol 98 (4) ◽  
pp. 140-148
Author(s):  
N.G. Kulikova ◽  
I.V. Volkova ◽  
A.S. Tkachenko
Keyword(s):  
B Lymphocytes ◽  
Blood Cells ◽  
White Blood Cells ◽  
Recurrent Infection ◽  
Recurrent Infections ◽  
T Suppressors ◽  
Children With Adhd ◽  
Therapeutic Measures ◽  
T Helpers ◽  
Head Area

The aim. to optimize the treatment of children with ADHD who often suffer from recurrent infections by justifying the effectiveness of a therapeutic and physiotherapy complex that includes a BOS-bioacoustic effect on the head area and NILI on the projection of the cubital area and thymus. Method of research. In children (140 people) with a verified diagnosis of ADHD, often suffering from recurrent infection, humoral-cellular immunity indicators (immunoglobulins: IgA; IgG; IgM); T-and B-lymphocytes; cytokines) were studied in the dynamics of the use of therapeutic measures. Depending on the applied physiotherapy method of treatment, the children were divided into 3 groups: the 1st group-received NILI on the projection of the cubital area and thymus, the 2nd – received BOS-bioacoustic effect on the head area, the 3rd – developed physiotherapy complex, including BOS-bioacoustic effect on the head area and NILI on the projection of the cubital area and thymus. Conclusion. the developed physiotherapy complex, including BOS-bioacoustic effect on the head area and NILI on the projection of the cubital area and thymus, can be considered as a therapeutic and preventive model of respiratory viral attacks in children with SSDVNS, since it reduces the number of recurrent viral attacks by 3.7 times, correcting the activity of circulating cytokines, T-lymphocytes (CD3+), T-helpers (CD4+) and T-suppressors (CD8+) in the population white blood cells.

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