scholarly journals Kualitas Ekstrak Karaginan Dari Rumput Laut “Kappaphycus alvarezii” Hasil Budidaya Di Perairan Pantai Kartini Dan Pulau Kemojan Karimunjawa Kabupaten Jepara

2017 ◽  
Vol 6 (2) ◽  
pp. 88 ◽  
Author(s):  
Endang Supriyantini ◽  
Gunawan Widi Santosa ◽  
Agus Dermawan

Karaginan merupakan senyawa hidrokoloid yang diekstraksi dari rumput laut merah jenis Kappaphycus alvarezii. Karaginan dapat digunakan untuk meningkatkan kestabilan bahan pangan baik yang berbentuk suspensi (dispersi padatan dalam cairan), emulsi (dispersi gas dalam cairan). Tujuan dari penelitian ini adalah mengetahui Kualitas ekstrak karaginan hasil ekstraksi rumput laut K. alvarezii dari dua lokasi sampling yang berbeda. Penelitian ini dilaksanakan pada bulan Agustus 2015 sampai bulan Januari 2016, di perairan Pantai Kartini, Jepara dan peraian Kemojan Karimunjawa, Jepara. Hasil penelitian menunjukkan analisis spektra FTIR produk telah memenuhi spesifikasi karaginan standar karena gugus-gugus fungsi yang terdapat pada spektrum sampel yang dihasilkan sama seperti gugus pada kappa karaginan yaitu gugus ester sulfat, OH, ikatan glikosidik. Hasil analisis sifat fisik-kimia karaginan (rendemen, kadar air, kadar abu, kadar sulfat, viskositas, dan kekuatan gel) yang paling baik yaitu karaginan dari perairan Kemojan Karimunjawa dibandingkan karaginan Pantai Kartini. Kadar sulfat Pantai Kartini dan Kemojan Karimunjawa masih dibawah kadar mutu yaitu 15-40 %, Sedangkan kekuatan gel kedua perairan yaitu Kemojan Karimunjawa 78,57 g.cm-2 dan Pantai kartini 61,86 g.cm-2 masih jauh di bawah baku mutu yaitu 685,50 g.cm2. Carrageenan is a hydrocolloid compounds extracted from red seaweed types Kappaphycus alvarezii. Carrageenan can be used to improve the stability of food in the form of suspension or emulsions. The purpose of this study was to determine the quality of the extract of carrageenan extracted from K. alvarezii at two different sampling locations. This research was conducted in August, 2015 and January, 2016. Samples of seaweed obtained from two different waters, namely Kartini Beach and Kemojan Karimunjawa Island, bolt at jepara regency. The results showed the FTIR spectra analysis of the product met the specifications for a standard carrageenan functional groups contained in the sample spectrum as expressed in clusters of kappa carrageenan namely sulphate ester group , OH , glycosidic bond. The results of analysis  of the physical-chemical properties of carrageenan ( yield, moisture content , ash content , sulphate content , viscosity and gel strength ) the best quality of carragenan was obtained from carrageenophyte taken from Kemojan Karimunjawa Island, although sulphate content from two different waters was under quality level at 15 – 40%, while the  gel strength kartini 61.86 g.cm-2 and Kemojan 78.57 g.cm-2 both were still far below the of carrageenophyte quality at 685.50 g.cm-2.

2019 ◽  
Vol 8 (4) ◽  
pp. 409-415
Author(s):  
Nursiana Suci Wulandari ◽  
Rini Pramesti ◽  
AB. Susanto

Kappaphycus alvarezii merupakan rumput laut merah penghasil karaginan yang dimanfaatkan dalam berbagai industri dan diperoleh melalui ekstraksi. Ekstraksi umumnya menggunakan larutan alkali sebagai pelarut dalam proses pemanasan maupun perendaman. Ekstraksi tanpa larutan alkali masih jarang dilakukan. Ekstraksi cara alkali, non-alkali maupun pre-treatment alkali mempengaruhi kualitas karaginan sehingga penelitian ini perlu dilakukan. Tujuan penelitian untuk memperoleh karaginan kualitas terbaik dari 3 metode ekstraksi. Parameter yang diambil meliputi rendemen, kadar air, kadar abu, kekuatan gel, viskositas dan analisis FTIR. Karaginan dihasilkan melalui ekstraksi secara alkali dengan pemanasan KOH, pre-treatment alkali dengan perendaman KOH sebelum pemanasan dan ekstraksi native pemanasan dengan air suling. Hasil penelitian didapatkan nilai rendemen terbaik dengan metode alkali (36,68±2,33%). Kadar air terbaik metode pre-treatment (3,91±0,84%). Kadar abu terbaik metode alkali (32,7±2,42%). Kekuatan gel terbaik dengan metode alkali (519±29,01g/cm2). Viskositas terbaik metode native (70,43±0,74 cPs). Hasil FTIR menunjukkan sampel yang dihasilkan jenis kappa karaginan. Kappaphycus alvarezii produces carrageenan which is widely used for industrial and obtained through extraction. Generally the extraction is using an alkaline solution as a solvent of heating or soaking process. Extraction without alkaline solution is infrequently performed. Different extraction methods affect the quality of the carrageenan, therefore the research has to be done. The purpose of this research is to obtain the best quality carrageenan from 3 different extraction methods. The parameters performed yield, water content, ash content, gel strength, viscosity and FTIR analysis. Carrageenan are produced through alkaline extraction treatment with KOH, pre-treatment alkaline with KOH and native extraction with distilled water. The results showed the best yield was alkaline method (36.68±2.33%), the best water content was pre-treatment method (3.91±0.84%), the best ash content was the alkaline method (32.7±2.42%), the best gel strength was the alkaline method (519 ± 29.01g/cm2), the best viscosity was the native method (70.43±0.74 cPs). Analysis FTIR showed the samples produced kappa carrageenan.


2021 ◽  
Vol 34 (3) ◽  
Author(s):  
HARYA BIMASUCI ◽  
RATIH IDA ADHARINI ◽  
DINI WAHYU KARTIKA SARI

Kappaphycus seaweed is the primary source of carrageenan, a polysaccharide widely utilised as a thickening and gelling agent in many commercial products. Unlike pure refined carrageenans, semi-refined carrageenans (SRC) are considered more cost-efficient and easier to produce. This study evaluates the quality of SRC extracted from Kappaphycus alvarezii (Doty) Doty ex Silva 1996, and Kappaphycus striatus (F. Schmitz) Doty ex P.C. Silva 1996, seaweeds farmed in the northern part of Java Island, particularly in Serang, Seribu Islands, and Karimunjawa Islands. Using 8 % KOH as a solvent, SRC was extracted from clean dried seaweed. Physical and chemical characteristics of the SRC were measured by assessing the percentage SRC yield, ash content, moisture, sulphate content, viscosity (cp) and gel strength (g.cm-2). Pearson’s correlation analysis was performed to investigate the relationship between SRC quality and environmental factors. Fourier transform infrared spectroscopy (FTIR) analysis showed that all samples had kappa-carrageenan from peak absorbance of 1218–1224 cm-1 indicating ester sulphate bonds, 924–925 cm-1 indicating 3,6–anhydrogalactose, and 843–844 cm-1 suggesting galactose–4–sulphate groups. Furthermore, ash content, moisture, and viscosity of SRC passed the FAO standard, while the sulphate content was below the threshold limit. Several environmental factors had a significant correlation with SRC quality, such as water pH positively correlated with gel strength, salinity level positively correlated with gel strength, and depth positively correlated with ash content. This study concluded that SRC produced from the sampling sites had varying qualities and may be affected by environmental parameters in their cultivation site.


2018 ◽  
Vol 154 ◽  
pp. 01034 ◽  
Author(s):  
Heri Heriyanto ◽  
Indar Kustiningsih ◽  
Denni Kartika Sari

Euchema cottonii is a good source of kappa-carrageenan and can be found cultivated in the Indonesia coastal areas in which one of them is in Banten Province. Carrageenans have many applications and are utilized in human food and pet-food industry. Carrageenans are also utilized in non-food industry such as pharmaceuticals, cosmetics, printing and textile formulations. Hence, the present study features on the cooking process cooking time and cooking temperature. The effects of these parameters on carrageenan quality such as gel viscosity and gel strength were studied. The process of extraction of carrageenan was conducted with variations temperature: 60, 70, and 80 °C and the variation of time: 1, 2, and 3 hours. Alkaline substance used was KOH with 8% concentration and the ratio of solvent to dry seaweed 8:1. From the present investigation, it was observed that SRC extraction reached the best condition at temperature 70 °C for 2 hours with the value of yield 30.20%, 5.90% moisture content, 18.34% ash content, sulfate content of 6.94%, viscosity of 190 cP, and the gel strength 714.45 g / cm2. The treatment of temperature and extraction time significantly affected the quality of the SRC yield parameter, viscosity and gel strength.


Author(s):  
Jamal Basmal ◽  
Diah Ikasari

Semi refine carrageenan (SRC) is generally produced by cooking dried Kappaphycus alvareziiinto hot alkali solution at temperature of 80ºC for 2 hours. However, the temperature fluctuationduring the production process affects to the increase of production cost and the reduction of theyield as well as the decrease of SRC quality. The improvement of this process can be achieved bysoaking immediately fresh K. alvarezii into cold alkali solution, called cold alkali technique. Inorder to obtain its optimum condition, modification of SRC production process using cold alkalitechnique had been conducted. Fresh K. alvarezii was soaked in 8% KOH at room temperaturefor 10, 20, 30, 40, and 50 days. The alkalinized K. alvarezii was then washed with fresh water untilpH reached to 7 – 8, chopped to the size of 3 – 5 mm and then dried under sun light. The SRCbeing produced was then evaluated for its quality. The result showed that the quality of SRC fromfresh seaweed K. alvarezii was affected by soaking time. The highest quality of SRC was obtainedby soaking K. alvarezii with 8% KOH solution for 50 days. This was indicated by the ester sulphatecontent of 6.74 %, water gel strength of 746 g/cm2, and moisture content of 11.73 %.


2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Andi Noor Asikin ◽  
Indrati Kusumaningrum ◽  
Doddy Sutono

Carrageenan is a gelling form of polysaccharide obtained from extraction process of red seaweeds (Rhodophyceae) including Kappaphycus alvarezii. Carrageenan is widely used for pharmacy, cosmetics,  food,  gelling,  and  binding  agent  as  well  as  emulsifier  and  stabi-lizer. The quality of carrageenan is affected by several factors, one of them such as concen-tration of KOH. The objectives of this research were to define the best concentration of KOH for extraction and characterize the functional properties. Functional properties of carrageen-an in this research were sulphate content, acid insoluble ash, gell strength, fiber, and whiteness. The experimental design used for this research was completely ran-domized design with four treatments of KOH concentrations i.e. 1%, 3%, 5%, and 7%.  The study showed that the use of KOH concentarion of 7% resulted the best of carrageenan quality containing 24.36% sulphate, 1.96% acid insoluble ash, 17.80 g/cm2 gell strength, 8.94% fiber and 58.91% whiteness. Keywords: carrageenan, extraction, functional properties, Kalium Hydroxide, Kappaphycus alvarezii


2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Sri Mulyani ◽  
F.M.C. Sigit Setya Budi ◽  
Yudi Pranoto ◽  
Umar Santoso

This study was aimed to observe the physical and chemical properties of buffalo hide as raw material for gelatin production by pretreatment using alkali-acid. Buffalo hide was observed on three sections, i.e. back (butt/croupon), abdomen (belly) and shoulder. The physical properties of the hide were analysed through the measurement of hide thickness, whereas the chemical properties were analysed by determining the proximate and amino acid composition. The qualities of gelatin (gel strength, viscosity and pH) were compared to standard commercial gelatin and Gelatin Manufactures Institute of America (GMIA). Gelatin extraction was done using a combination pretreatment alkali (0.5M NaOH) and acid (0,9M HCl). The results showed that butt section had collagen density was best compared to shoulder and belly. The thickness of butt (10.219 ± 0.318 mm) smaller than the shoulder (11.365 ± 0.389 mm), but thicker than the belly. The protein and hydroxyproline content on the butt was the highest, resulting the value 27.517 ± 0.049% and 97.330 ± 0.331 ppm, respectively. Extraction using 0.9 M hydrochloric acid produced yield 29.17 ± 2.12%. The gel strength 242.35 ± 12.90 g Bloom, viscosity 16.37 ± 1.5 cp and pH 5.15 ± 0.05. The gelatin quality meets the standards of quality of GMIA (2012) even better than commercial gelatin.


2018 ◽  
Vol 3 (1) ◽  
pp. 3-12 ◽  
Author(s):  
Elise D’Huart ◽  
Pauline Lider ◽  
Jean Vigneron ◽  
Béatrice Demoré

AbstractBackgroundStabilis®is an international database on stability and compatibility of drugs. The stability data comes mainly from publications of pharmaceutical journals. As the quality of the published stability studies is not equivalent, the objective of this work was to propose a level of evidence for the physico-chemical stability studies selected for the database.MethodsAt first, we evaluated the main pharmacological class consulted by the users. This work was then divided into 5 steps: (1) updating of the criteria to validate a stability study, (2) creating a grid rating articles, (3) rating of the articles of stability studies for anticancer, antifungal and antiviral drugs by 2 evaluators, (4) creation of new screens in the database to enter rating, to visualize the pictograms and commentaries by the users, (5) creation of a guideline to explain the different levels of evidence.ResultsThe main pharmacological class consulted by Stabilis®users is the anticancer drugs and then antiinfectives. We have selected anticancer, antifungal and antiviral drugs for our study. Two hundred and forty publications were evaluated. The highest level attributed was A for anticancer and antifungal drugs and C for antiviral drugs. This difference can be explained by the fact that the majority of publications about antiviral drugs were older.The most frequent anomalies in the rating of articles were an incomplete or imperfect validation of the analytical method (high value of the coefficient of variation) and a defect in the evaluation of the stability indicating capacity.It must be noted that the level of evidence is not the quality level of the analytical method but the mixture of the quality of the method validation and of the results. This aspect was a choice of the Stabilis®team and seems important because the security of the patient is impacted by both aspects.ConclusionsThis new function contributes to help the Stabilis®users to evaluate the stability data published and to take a decision for their use in daily practice. This function will be progressively extended to other pharmacological classes of injectable drugs and then for non-injectable preparations.


2020 ◽  
Vol 31 (2) ◽  
pp. 95-102
Author(s):  
Lalu Danu Prima Arzani ◽  
◽  
Tjahja Muhandri ◽  
Nancy Dewi Yuliana ◽  
◽  
...  

Kappaphycus striatum and Kappaphycus alvarezii are kappa carrageenan-producing seaweeds. K. striatum is also a source of iota carrageenan. The study was aimed to evaluate the effects of extraction time and alkali concentration on the characteristics of semi refined carrageenan (SRC) produced from seaweeds K. striatum and K. alvarezii. The SRC was extracted prepared by cooking dried seaweed samples in KOH solution (ratio seaweed:KOH solution= 1:40) with KOH concentrations of 6, 8 and 10% for 60 and 120 minutes at 70°C. The results showed that the best SRC characteristics produced from K. striatum seaweed were obtained from the extraction process at 6% KOH for 60 minutes which yielded 46.05±0.16%, carrageenan with gel strength of 362,07±21,44 g/cm2 and viscosity at 70°C 74 cP. Meanwhile the best characteristics of SRC produced from K. alvarezii seaweed were obtained from extraction in 10% KOH for 60 minutes having the highest yield of 39,03±0.95%, gel strength 1130.67± 16.34 g/cm2 and viscosity at 70°C of 59 cP. FTIR analysis showed that the functional groups which are kappa carrageenan and iota carrageenan characteristics were found in both samples at wave numbers of 846.97-847.09 and 800.53-801.29 cm-1, respectively.


2019 ◽  
Vol 14 (1) ◽  
pp. 9
Author(s):  
Mat Fahrur ◽  
Andi Parenrengi ◽  
Makmur Makmur ◽  
Sri Redjeki Hesti Mulyaningrum

Peremajaan bibit melalui seleksi dapat dilakukan untuk perbaikan kualitas bibit rumput laut. Penelitian ini bertujuan untuk menentukan performa bibit rumput laut K. alvarezii hasil seleksi varietas Takalar, SIRICA, dan Bali. Desain penelitian adalah rancangan acak lengkap (RAL), dengan empat siklus pemeliharaan sebagai ulangan. Seleksi bibit dilakukan pada ketiga varietas dengan bibit non-seleksi sebagai kontrol internal. Pemeliharaan dilakukan di perairan Laikang, Takalar, Sulawesi Selatan selama empat siklus dengan durasi 30 hari/siklus. Pengukuran laju pertumbuhan harian (LPH), kandungan karagenan, kekuatan gel, dan monitoring kualitas air dilakukan setiap 30 hari. Data yang diperoleh dianalisis menggunakan analisis ragam (ANOVA). Hasil penelitian menunjukkan bahwa seleksi bibit berpengaruh signifikan terhadap LPH rumput laut (P<0,05). Rumput laut hasil seleksi memiliki LPH lebih tinggi, yakni: Bali (4,75 ± 0,04%/hari), SIRICA (4,74 ± 0,06%/hari), dan Takalar (4,62 ± 0,12%/hari) dibandingkan dengan kontrol internal Bali (3,94 ± 0,15%/hari), SIRICA (3,74 ± 0,10%/hari), dan Takalar (3,66 ± 0,32%/hari). Rumput laut hasil seleksi varietas Bali dan SIRICA memiliki kandungan karagenan yang relatif sama yakni (37,27 ± 6,68%) dan (37,08 ± 7,17%). Varietas Takalar memiliki kandungan karagenan yang rendah (31,84 ± 5,32%) namun memiliki kekuatan gel yang paling tinggi (570,03 ± 145,72 g/cm²) dibandingkan varietas Bali (444,39 ± 157,44 g/cm²) dan SIRICA (438,48 ± 72,70 g/cm²).Regeneration by selection method can improve the quality of seaweed seed. This study was aimed to determine the performance of selected seaweed seeds of K. alvarezii from Takalar, SIRICA, and Bali varieties. The experiments were arranged in a completely randomized design with four cycles cultivations as replications. In each variety, a batch of non-selected/regular seeds was cultivated serving as internal control. The seaweed was cultivated in Laikang waters, Takalar Regency, South Sulawesi for 30 days for each cycle. The measurements of daily growth rate (DGR), carrageenan yield, gel strength, and water quality were conducted every 30 days. The collected data were statistically analyzed using ANOVA. The results showed that the selected seeds showed faster growth than that of its respective control (P<0.05). The selected seed of three varieties of K. alvarezii had higher DGR: Bali (4.75 ± 0.04%/day), SIRICA (4.74 ± 0.06%/day), and Takalar (4.62 ± 0.12%/day) than that of control varieties, Bali (3.94 ± 0.15%/day), SIRICA (3.74 ± 0.10%/day), and Takalar (3.66 ± 0.32%/day). The selected seeds of Bali and SIRICA had similar carrageenan content (37.27 ± 6.68%) and (37.08 ± 7.17%), respectively. Takalar had the lowest carrageenan yield (31.84 ± 5.32) but had the highest gel strength (570.03 ± 145.72 g/cm²) compared to Bali (444.39 ± 157.44 g/cm²) and SIRICA (438.48 ± 72.70 g/cm²).


KOVALEN ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 146-154
Author(s):  
Asri Jaya ◽  
Ni Ketut Sumarni ◽  
Ahmad Ridhay

Research on extraction and characterization of crude carrageenan from seaweed (eucheuma cottoni) has been carried out. The research aims to determine the optimum extraction time and temperature of crude carrageenan extract from seaweed (eucheuma cottoni). Crude Carrageenan was extracted from seaweed (eucheuma cottoni) by dissolving crushed seaweed in 8% KOH with variation  of  extraction times are 1 hour, 1.5 hours, 2 hours, 2.5 hours and 3 hours, and temperatures are 50, 70, 90, 110 and 130oC. The results were analyzed using a Completely Randomized Design (CRD). The results show that the optimum extraction time in producing the best quality of crude carrageenan is 2 hours at 50oC with percentage is 78.4%, viscosity is 9.155 cP, sulphate content is 3.102% and gel strength is 7 g. Keywords : eucheuma cottoni seaweed, crude carrageenan, characterization


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