scholarly journals Identification of proteins in sensitive and tolerant lines of sunflower (Helianthus annus L.) under water deficit

2021 ◽  
Vol 117 (2) ◽  
pp. 1
Author(s):  
Babak MAGHSOUDI DAMAVANDI ◽  
Shahram LAK ◽  
Mehdi GHAFFARI ◽  
Mojtaba ALAVIFAZEL ◽  
Tayeb SAKINEJHAD

<p>The importance of examining environmental stresses and their role in predicting and evaluating the growth and yield of crops is very evident. Environmental stresses are the most important factor in reducing agricultural yields worldwide. In order to evaluate the environmental impact of water loss on the amount of proteins affected in sunflower, an experiment was conducted in randomized complete block design with three replications at Karaj Oil Seeds Research Institute. In order to study the response of susceptible line (BGK221) and tolerant line (RGK46) under drought stress conditions, extraction of protein by acetone deposition method was performed in 8 leaves and of 3 seedlings in each replication. The amount of extracted protein was determined by Bradford method using two-dimensional electrophoresis and existence of significant difference between the bands in sensitive and tolerant lines was investigated. A total of 467 repeatable bands were found in the tolerant line and 417 repeatable bands appeared in the sensitive line. Among these proteins, 6 bands in tolerant line (No 503, 1901, 904, 3301, 7011, 9005) and 6 bands in sensitive line (No 704, 811, 3205, 4108, 7307, and 9207) were significantly affected by drought stress. In both sensitive and tolerate lines the main consequence is increase in amount of protein. The results showed that the most important factor of tolerant line adaptive for environmental stress conditions is maintaining normal cell metabolism, keeping moisture in the cell, strengthening cellular structure and antioxidant defense. The study also showed that drought stress had the greatest effect on cytoplasmic and nucleus proteins, metabolism and energy of proteins</p>

2016 ◽  
Vol 10 (1) ◽  
pp. 53-62
Author(s):  
Visca R Yuanita ◽  
Tri Kurniastuti ◽  
Palupi Puspitorini

Research about influence of goat manure and NPK fertilizer on the growth and yield of green eggplant (Solanum mengolena L.) has been conducted in field village farmer-owned Semen Gandusari District of Blitar which took place in March-April 2016. The aim of this study was to know the interaction for among goat manure and NPK fertilizer on the growth and yield of green eggplant as well as the effect of each factor. This study uses Randomaized Complete Block Design (RCBD) factorial consisting of 16 treatment and each 3 replicates ie: P0M0, P0M1, P0M2, P0M3, P1M0, P1M1, P1M2, P1M3, P2M0, P2M1, P2M2, P2M3, P3M0, P3M1 , P3M2, P3M3. The data taking were plant height, leaf number, fruit length, fruit diameter and weight of fruit per plant. The data were analyzed to F test as followed by a 5% test Honestly Significant Difference (HSD) error level of 5%. The experimental results very significant effect on plant height, leaf number, fruit length and weight of fruits per plant and the effect is not noticeable to the diameter of fruit plants green eggplant. The combined use goat manure 750 g / plant and NPK fertilizer 60 g / plant (P2M3) is proper fertilization to improve the growth and best yield of green eggplant with the highest total weight of the fruit crop that yields 1576.6 grams


PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0259585
Author(s):  
Gull Mehak ◽  
Nudrat Aisha Akram ◽  
Muhammad Ashraf ◽  
Prashant Kaushik ◽  
Mohamed A. El-Sheikh ◽  
...  

Optimum water availability at different growth stages is one the major prerequisites of best growth and yield production of plants. Exogenous application of plant growth regulators considered effective for normal functioning of plants under water-deficit conditions. A study was conducted to examine the influence of exogenously applied L-methionine on sunflower (Helianthus annuus L.) plants grown under water-deficit conditions. Twenty-five-day old seedlings of four sunflower cultivars, FH331, FH572, FH652 and FH623 were exposed to control (100% F.C.) and drought stress (60% F.C.) conditions. After 30-day of drought stress, L-methionine (Met; 20 mg/L) was applied as a foliar spray to control and drought stressed plants. Water deficit stress significantly reduced shoot fresh and dry weights shoot and root lengths, and chlorophyll a content in all four cultivars. While a significant increase was observed due to water deficiency in relative membrane permeability (RMP), malondialdehyde (MDA), total soluble proteins (TSP), total soluble sugars (TSS), ascorbic acid (AsA) and activity of peroxidase (POD). Although, exogenously applied Met was effective in decreasing RMP, MDA and H2O2 contents, it increased the shoot fresh weight, shoot length, chlorophyll a, chlorophyll a/b ratio, proline contents and the activities of SOD, POD and CAT enzymes in all four cultivars under water deficit stress. No change in AsA and total phenolics was observed due to foliar-applied Met under water stress conditions. Of all sunflower cultivars, cv. FH-572 was the highest and cv. FH-652 the lowest of all four cultivars in shoot fresh and dry weights as well as shoot length under drought stress conditions. Overall, foliar applied L-methionine was effective in improving the drought stress tolerance of sunflower plants that was found to be positively associated with Met induced improved growth attributes and reduced RMP, MDA and H2O2 contents under water deficit conditions.


2021 ◽  
Vol 13 (10) ◽  
pp. 45
Author(s):  
Naheif E. Mohamed ◽  
Ismail M. Bedawy ◽  
Yasser A. M. Hefny

To study genetic analysis of some physiological traits of drought stress in wheat using diallel techniques, an experiment was performed on ten bread wheat genotypes as parents and their 45 F1 hybrids in a randomized complete block design with three replicates under well-watered and drought stress conditions at the Research Farm of Faculty of Agriculture, Sohag University, Egypt during season of 2018/19. The results showed significant differences between the genotypes (G), Parents (P), F1 crosses, P vs. F1, GCA and SCA under well-watered and drought stress in the flag leaf area (FLA), flag leaf chlorophyll content (FLCC) and flag leaf temperature (FLT), except FLCC for F1 crosses exhibited insignificant differences. The significant differences were found in the interaction of SCA &times; Env., in all studied traits and GCA &times; Env., for FLT., indicating the involvement of both additive and dominance gene action in their inheritance. The most desirable heterotic effects were considered as the largest positive heterosis estimates for FLA and FLCC, and the lowest negative for FLT. The parent numbers P9, P8 and P3 were the best general combiner for FLA under normal irrigation and drought stress. While the parents P2, P7 and P9 were the best general combiner for FLCC, under normal irrigation and (P1, P2 and P3) under drought stress. Therefor the parents P1, P4 and P5 were the best general combiner for FLT under normal irrigation, also the P3, P4 and P5 were the best general combiner for FLT under drought stress conditions. Under normal irrigation and drought stress conditions as well as the combined data, the additive genetic components of variation (VA) in F1 &rsquo;s was much greater than dominance component (VD), as expressed by the (VA/VD) ratio which was more than unity for the FLA, and FLT under normal irrigation and their combined. This indicates that the additive gene effects in F1 crosses are more important than dominance and plays the major role in the inheritance of these studied traits.


2021 ◽  
Vol 46 (2) ◽  
pp. 232
Author(s):  
Maria Ulfa ◽  
Hadi Pranoto ◽  
Susylowati Susylowati

This research aims to find out 1) the interaction between the medium of planting and the concentration of nutrient solutions to the growth and yield of celery plants hydroponically. 2) types of planting media that can give different influences on the growth and yield of celery plants, and 3) concentration of nutrient solution that provides the best growth and yield of celery plants in different planting media.The research was conducted from December 2019 to February 2020 at the Integrated Laboratory of the Faculty of Agriculture of Mulawarman University, Samarinda. The research used factorial exsperiment 3×4 on Randomized Completely Block Design (RCBD), replicated three times with each treatment consisting of three plants. The first factor is the planting medium consisting of rockwool, charcoal husk, and combination of charcoal husk + tea amps. The second factor is the concentration of AB Mix nutrient solution consists of 500, 1,000, 1,500, and 2,000 ppm. The data was analyzed with test F and continued Least Significant Difference (LSD) test with a level of 5%.  The results showed that there is an interaction between planting media and concentration of nutrient solution in the growth and yield of celery plants. The interaction between the combination of charcoal planting media + tea pulp and concentration of 1,500 ppm is not real with the interaction of rockwool planting media and concentration of 1,500 ppm. From various types of planting media used, charcoal husk planting media provides the best influence on the growth and yield of celery plants. Based on polynomial orthogonal test the concentration of nutrient solution with an optimum point of 1,460.84 ppm affects the growth and yield of celery plants with a maximum dry weight of 2.14 g.


2015 ◽  
Vol 19 (1) ◽  
pp. 33-39
Author(s):  
MR Karim ◽  
MM Rashid ◽  
MA Salam ◽  
MA Mazid ◽  
MA Momin ◽  
...  

Correction: In the last line of the abstract, the word 'increase' was replaced with the word 'reduced' on 25th November 2015. The correct sentence now reads "The application of PRH, Calsol and Magic Growth significantly reduced rice yield than that obtained with recommended fertilizer dose."AbstractInherent soil fertility and application of exogenous fertilizer generally affect rice yield. Fertilization would be soil fertilization or foliar feeding through leaf area. Plant growth promoting substances also improve plant performance through modulation of its growth and yield. The study was conducted at BRAC Agricultural Research and Development Center (BARDC), Gazipur. The aim of the study was to find out the growth and yield of Boro rice as influenced by plant revitalization hormone (PRH) and liquid fertilizer Calsol and Magic Growth during dry season 2012-13 using randomized complete block design with three replications. Treatment consists of; T1=Recommend fertilizer dose of NPKS and Zn (Control), T2=T1+ recommended PRH, T3=T2 + half of the PRH, T4=Half of doses of T1 + PRH, T5=PRH, T6=Recommended fertilizer dose of NPK with three N top dressed along with Calsol, T7=Recommended fertilizer dose of NPK with 1st and 3rd split of N application and along with three split of Calsol application, T8=Recommended dose of liquid fertilizer Magic Growth. The effect of different treatment combinations of PRH and liquid fertilizer Calsol and Magic Growth showed significant difference (p<0.05) in panicle m-2, unfilled grain panicle-1, total grain panicle-1, and grain yield. However, none of the treatments out yielded the existing fertilizer application method. The application of PRH, Calsol and Magic Growth significantly reduced (corrected on 25th Nov. 2015) rice yield than that obtained with recommended fertilizer dose.Bangladesh Rice j. 2015, 19(1): 33-39


2015 ◽  
Vol 4 (1) ◽  
pp. 73
Author(s):  
Ogundare S. K. ◽  
Mohammed S. A. ◽  
Owolabi J. F.

Experiments were carried out at the student’s experimental field, Kabba College of Agriculture, Horticulture section to examine the effect of soil application of cow dung combined with foliar application of boost extra on growth and yield of okra in an ultisol, Nigeria. The land was ploughed each year and harrowed with the aid of tractor mounted implements. The experiment was laid out in a randomized complete block design (RCBD). The treatments consisted of A = 3t/ha cow dung, B = 1.0 L/ha foliar + 2.5t/ha cow dung, C =1.5L/ha foliar + 2t/ha cow dung, D    = 2 L/ha foliar      + 1.5 t/ha cow dung, E = 2.5L/ha foliar + 1.0t/ha cow dung, F = 3t/ha foliar. Each year experiment was conducted using a single field having dimension of 35 by 14m which was laid out into three blocks with 1m guard row between blocks. Each block consists of six plots (5 by 4m) and 1m guard row between plots. Cow dung manure was applied a week before planting. Okra variety Lady’s finger was used. Three seeds per hole were planted on April 4th in both years on the flat with a spacing of 60cm x 25cm between and within the rows and later thinned to one plant per stand. Data taken included plant height at 50 % flowering, number of branches per plant, leaf area, pod length, pod diameter, number of pods per plant; and pod weight and yield (t/ha). The data were subjected to Analysis of Variance (ANOVA) while the Least Significant Difference (LSD) was used to separate treatment means. The result shows that plot treated with 2.0L/ha foliar + 1.5 t/ha cow dung had the best performance in yield and yield components in this study. It is therefore recommended that okra farmers should integrated foliar fertilizer (boost extra) at the rate of 2L per hectare with cow dung at rate of 1.5t per hectare be used for okra production in the study area.


2021 ◽  
Vol 910 (1) ◽  
pp. 012112
Author(s):  
Wesam Hamad Al-Jubory ◽  
Amer Badawy Al-Jubory ◽  
Akeel Najim AL Mohammdedi

Abstract A field experiment was carried out during the 2020-2021 winter season, in Al-Alam district, east of Tikrit, to determine the effect of plant spacing and spraying with zinc in the growth and yield of the fennel. The experiment study of the effect of different distances are (10,15 and 20 cm) and three concentrations are (0,15 and 20 g/l). The experiment carried out within the system of testing the factorial experiment has been used according to random complete block design (R.C.B.D) with three blocks. Considered statistically analyzed the data according to the design of and experience and compared to averages of transactions based on test least significant difference (L.S.D) 5% Level of moral study results showed. The planting distance of 10 cm gave the highest rate of plant height reached 71.41 cm, and the planting distance of 20 cm showed a significant increase in the two characteristics of the number of flowers in the main flower and the number of flowering inflorescences in the plant with the increase in planting distance reached 197.75/flower and the highest rate of the number of flowering reached 16.89 inflorescences/flower. The result showed the spray treatment with a concentration of 15 g/liter was superior in the characteristic of the number of flowers in the main inflorescence and the number of flowering inflorescences in the plant, respectively.


2021 ◽  
Vol 910 (1) ◽  
pp. 012031
Author(s):  
Hanan A.k. Al-Nafei ◽  
Maher H.S. Al-Mohammad

Abstract A field experiment was carried out at winter season of 2020-2021 in Babylon Governorate, region of Bernon, at 8 km north-east of Hilla town between 44.27 ° north and 36.32 ° east latitude, to study the effect of planting distance and Humic acid on growth, yield and antioxidant activity of petals and seeds of the safflower, the treatments were distributed in a factorial experiment with randomized completely block design (RCBD) with three replicates, the first factor was the distance between plants (10, 15 and 20 cm) and second was spraying Humic acid at concentrations (0, 125, 250 and 500 mg.l-1), data were collected and means were tested according to least significant difference test (LSD) at probability level 0.05. The results showed superiority significant of planting distance at 20 cm and spraying humic acid at concentration 500 mg.L-1 on growth and yield parameters, while plants distance at 10 cm excelled on plant height and content of petals and seeds of antioxidant activities. The results showed superiority significant at interaction treat 20 cm × 500 mg.L-1, it produced the highest rates in most considered qualities and yield of dry petals and seed yields were 79.658 and 2197.5 kg. ha-1, while interference treat 10 cm x 500 mg.L-1 produced the highest means on total phenolic compounds and total oxidative activity and scavenging free radicals reached 41.47, 212.7, 58.07, 38.72 and 33.59 mg.g-1 DW, 67.7 and 47.0% in petals and seeds respectively.


2020 ◽  
pp. 36-42
Author(s):  
E. Ndor ◽  
U. D. Faringoro

Field trials were conducted during 2017 and 2018 rainy season, to investigate the effect of nitrogen fertilizer rates and time of application on the growth and yield of cowpea. The treatment consisted of three levels of urea: 0, 40, 80 kg N ha-1 and the times of fertilizer application: during planting of cowpea, 2 weeks after planting, 4 weeks after planting and 6 weeks after planting; which were factorially combined to form twelve treatments and laid in Randomized Complete Block Design (RCBD). The results showed that, Nitrogen fertilizer rates and time of application had a significant increased on all the growth and yield parameters of cowpea in both years of trials. Application of 80 kg N ha-1 produced the highest number of cowpea leaves (134.45 and 139.23), number of branches (9.45 and 10.54), vine length (78.23cm and 80.12cm) and highest seed weight of 0.52 t ha-1 in both 2017 and 2018 trials. However, the control plots produced plants with the highest number of root nodules (14.78 and 12.49) in both years of cropping. Also, application of nitrogen fertilizer at two weeks after planting of cowpea produced the highest growth and seed weight of 0.68 t ha-1 in 2017 cropping; while in 2018 trial, application of nitrogen fertilizer at two weeks after cowpea planting produced seed yield of 0.69tha-1 which is statistically similar with application of nitrogen fertilizer on the fourth week after planting which produced 0.55 t ha-1 of cowpea seeds. The interaction between nitrogen fertilizer application rates and time of application did not showed any significant difference in cowpea yield and yield components in both years of cropping.


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