Impact of enriching larval brine shrimp (Artemia sp.) with a supplement containing polyunsaturated fatty acids on their growth and mortality

Abstract The aim of the study was to determine the impact the commercial supplement S.presso (INVE Aquaculture, Belgium), a source of polyunsaturated fatty acids to enrich the proximate composition of brine shrimp (Artemia sp.), had on their growth and mortality. Four different enrichment protocols were investigated: the supplement was given in one or two doses at various time intervals. The results indicate that while S.presso increased nauplius mortality slightly, administering the appropriate dose reduced losses to a very low level (from 12.5% with a single dose to 8.6% with the recommended dose divided into to equal parts). Additionally, it was confirmed that the supplement S.presso increased brine shrimp growth slightly in comparison to that of the control group, but the differences were statistically insignificant. The results permit concluding that S.presso is suitable for use as a source of polyunsaturated fatty acids for brine shrimp enrichment with the intent of using them as fish food.

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Correction: N-3 Polyunsaturated Fatty Acids (PUFAs) Reverse the Impact of Early-Life Stress on the Gut Microbiota

PLoS ONE ◽

10.1371/journal.pone.0142228 ◽

2015 ◽

Vol 10 (10) ◽

pp. e0142228 ◽

Cited By ~ 6

Author(s):

Matteo M. Pusceddu ◽

Sahar El Aidy ◽

Fiona Crispie ◽

Orla O’Sullivan ◽

Paul Cotter ◽

...

Keyword(s):

Fatty Acids ◽

Gut Microbiota ◽

Polyunsaturated Fatty Acids ◽

Life Stress ◽

Early Life ◽

Early Life Stress ◽

The Impact

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Effects of Dietary Addition of Linseed Oil on the Content of Polyunsaturated Fatty Acids in Egg Yolk of Gallus domestiaus

Journal of Biobased Materials and Bioenergy ◽

10.1166/jbmb.2021.2089 ◽

2021 ◽

Vol 15 (4) ◽

pp. 565-570

Author(s):

Suhu Duan ◽

Zaiqiang Li ◽

Zhenzhen Fan ◽

Mengran Qin ◽

Xiaoxue Yu ◽

...

Keyword(s):

Fatty Acids ◽

Gas Chromatography ◽

Polyunsaturated Fatty Acids ◽

Theoretical Basis ◽

Linseed Oil ◽

Egg Yolk ◽

Free Food ◽

Control Group ◽

Experimental Group ◽

Group 1

In order to study the effect of dietary supplement of linseed oil on polyunsaturated fatty acids (PUFAs) in egg yolk of Gallus domestiaus, total 160 healthy Gallus domestiaus of 26-week old were randomly selected and divided into 4 groups, each of which included 40 chickens fed outdoors with an area of 63 m2 and free food and drinking water. The control group (CK) was fed the basic diet, and the experimental group was added 1, 3 and 5% of linseed oil in the fundamentals of diet (experimental group 1, 2 and 3, abbreviated as P1, P2, P3), respectively. The content of PUFA and the value of n-6 PUFA and n-3 PUFA in egg yolk were determined by gas chromatography with 24 eggs randomly selected in each group after 10th, 25th and 40th days of the commence of the experiment. We found that the levels of α-linolenic acid (C18:3n3, ALA) and docosahexaenoic acid (C22:6n3, DHA) in egg yolk were clearly higher than the CK when 3% linseed oil or 5% linseed oil were added in the diet during the three experiment periods, in which the contents of linoleic Acid (18:2n6c, LA) and arachidonic acid (C20:4n6, ARA) in egg yolk differ little between P1, P2 and P3 and the CK while the value of n-6 PUFA and n-3 PUFA in egg yolk of the P2 and P3 groups decreased significantly. The comprehensive comparisons suggested that the content of C18:3n3 and C22:6n3 in egg yolk could be clearly increased in the P2 while the value of n-6 PUFA and n-3 PUFA decreased. This study provides theoretical basis for the production of functional eggs enriched with C22:6n3 and C18:3n3.

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Effect of n-3 long-chain polyunsaturated fatty acids supplementation in healthy mothers on DHA and EPA profiles in maternal and umbilical blood: a randomized controlled trial

Journal of Perinatal Medicine ◽

10.1515/jpm-2018-0155 ◽

2019 ◽

Vol 47 (2) ◽

pp. 200-206

Author(s):

Dragan Soldo ◽

Matija Mikulić-Kajić ◽

Lara Spalldi Barišić ◽

Nikolina Penava ◽

Martina Orlović ◽

...

Keyword(s):

Fatty Acids ◽

Randomized Controlled Trial ◽

Corn Oil ◽

Controlled Trial ◽

Lipid Extraction ◽

Control Group ◽

Randomized Controlled ◽

Umbilical Blood ◽

Epa And Dha ◽

The Impact

AbstractBackgroundThe objective of the study was to compare the effect of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) dietary supplementation on their concentration in total lipids (TL) and lipid fractions of maternal and umbilical vein (UV) blood. The specific objective was to analyze the impact of EPA and DHA supplementation on pregnancy outcome and neonatal birth weight.MethodsWomen were randomly single-blinded (randomized controlled trial; ISRCTN36705743) allocated to the group receiving EPA and DHA supplementation (supplemented group) or the group receiving placebo-corn oil (control group) in the time period from January 1st, 2016 until March 1st, 2017. Women in the supplemented group (n=45) took 360 mg EPA and 240 mg DHA daily while controls (n=42) were given a placebo. Maternal and UV bloods were obtained at delivery. After lipid extraction, phospholipids (PL), cholesterol esters (CE), triacylglycerols (TG) and non-esterified fatty acids were separated by thin layer chromatography and analyzed by gas chromatography.ResultsHigher DHA concentrations in TL (37.24±21.87 mg/L), PL (13.14±8.07 mg/L) and triacylglycerols (2.24±2.21 mg/L) were recorded in mothers from the supplemented group when compared to the study group (TL 21.89±14.53 mg/L; P<0.001; PL 9.33±5.70 mg/L; P=0.013; TG 0.56±0.43 mg/L; P<0.001). Higher DHA concentrations in UV samples were found in TL (11.51±7.34 mg/L), PL (5.29±3.31 mg/L) and triacylglycerols (0.62±0.46 mg/L) from the supplemented groups compared with controls (TL 7.37±3.60 mg/L; P=0.002; PL 3.52±2.19 mg/L; P=0.005; TG 0.40±0.46 mg/L; P=0.035). The ratio of AA:DHA was lower in maternal (2.43) and UV serum (4.0) of the supplemented group than in the control group (maternal 3.85 P<0.001; UV 4.91 P<0.001).ConclusionThe study demonstrated the higher ratio of AA/DHA in the control group indicating that pregnant women on the traditional Herzegovina diet need supplementation with DHA and EPA.

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Effect of mono and polyunsaturated fatty acids on muscle cholesterol level in fattening pigs

Biotechnology in Animal Husbandry ◽

10.2298/bah0502049p ◽

2005 ◽

Vol 21 (1-2) ◽

pp. 49-54 ◽

Cited By ~ 1

Author(s):

M. Pieszka ◽

T. Barowicz ◽

W. Migdalt ◽

Branislav Zivkovic

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Total Cholesterol ◽

Cholesterol Level ◽

Linseed Oil ◽

Cholesterol Content ◽

Control Group ◽

Longissimus Muscle ◽

Fresh Tissue ◽

Fattening Pigs

The effect of supplementing fattening pigs with oils differing in MUFA (monounusaturated fatty acids) and PUFA (polyunsaturated fatty acids) level addition for fatteners diets on the cholesterol content of m. longissimus was investigated. In a feeding trial with 48 crossbred pigs [? ($ White Polish Large x S Polish Landrace) x <$ Pietrain] randomly assigned to four treatments groups of 12 animals each (6 gilts and 6 barrows), dietary supplements of 3% maize, sunflower or linseed oil in the experimental groups and of 3% lard in the control group were applied. Total cholesterol in the longissimus muscle of linseed oil supplemented pigs was found to decrease significantly (PO.01) compared to the lard-fed pigs (60.75 vs. 71.37 mg/100 g of fresh tissue). Similar tendencies for reduced cholesterol level in longissimus muscle of sunflower and maize oil supplemented pigs were also noted. The production of pork with reduced total cholesterol seems to be important in terms of consumer health and culinary value.

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The Impact of Pre-emptive Large Dose of Methylprednisolone Combined With Gabapentin on Pain Treatment and Convalescence After Total Knee Arthroplasty in Elderly. A Randomized Control Study

10.21203/rs.3.rs-149103/v1 ◽

2021 ◽

Author(s):

Henryk Liszka ◽

Małgorzata Zając ◽

Artur Gądek

Keyword(s):

Total Knee Arthroplasty ◽

Single Dose ◽

Knee Arthroplasty ◽

Opioid Analgesic ◽

Control Group ◽

Study Group ◽

Intensity Level ◽

Old Patients ◽

Total Knee ◽

The Impact

Abstract Background The aim of the study was to assess whether administration of gabapentin and methylprednisolone as “pre-emptive analgesia” in a group of patients above 65 years of age would be effective in complex pain management therapy following total knee arthroplasty (TKA). Material and Methods One hundred seventy, above 65 years old patients were qualified for the study, and 10 patients were excluded due to clinical situation. One hundred sixty patients were double-blind randomized into two groups: the study (eighty patients) and controls (eighty patients). The study group received as “pre-emptive” analgesia a single dose of 300 mg oral gabapentin and 125 mg intravenous methylprednisolone, while the others placebo. Perioperatively, all the patients received opioid and nonopioid analgesic agents calculated for 1 kg of body weight. We measured the levels of inflammatory markers (leukocytosis, C-reactive protein - CRP), pain intensity level at rest (numerical rating scale - NRS), the life parameters and all complications. Results Following administration of gabapentin and methylprednisolone as “pre-emptive” analgesia CRP values being lower on all postoperative days ( 1, 2 days - p<0,00001, 3 day – p=000538), leukocytosis on day 2 (p<0,0086) and 3 (p<0,00042), the NRS score at rest 6, 12 (p<0,000001), 18 (p<0,00004) and 24 (p=0,005569) hours postoperatively . Methylprednisolone with gabapentin significantly decreased the dose of parenteral opioid preparations (p=0,000006). The duration time of analgesia was significantly longer in study group (p<0,000001). No infectious complications were observed; in the control group, one patient manifested transient ischaemic attack (TIA). Conclusion The use of gabapentin and methylprednisolone at a single dose decreases the level of postoperative pain on the day of surgery, the dose of opioid analgesic preparations, the level of inflammatory parameters without infectious processes.

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Improvement of the probiotic effect of micro-organisms by their combination with maltodextrins, fructo-oligosaccharides and polyunsaturated fatty acids

British Journal Of Nutrition ◽

10.1079/bjn2002634 ◽

2002 ◽

Vol 88 (S1) ◽

pp. S95-S99 ◽

Cited By ~ 111

Author(s):

A. Bomba ◽

R. Nemcová ◽

S. Gancarcíková ◽

R. Herich ◽

P. Guba ◽

...

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Dietary Lipids ◽

Control Group ◽

Jejunal Mucosa ◽

Gastrointestinal Microbiota ◽

Weanling Piglets ◽

Micro Organisms ◽

Hydrolysis Of ◽

Gnotobiotic Piglets

Probiotics could represent an effective alternative to the use of synthetic substances in nutrition and medicine. The data concerning the efficacy of probiotics are often contradictory. This paper focuses on the enhancement of the efficacy of probiotics by their combination with synergistically acting components of natural origin. Maltodextrins can be obtained by enzymatic hydrolysis of starch and are suitable for consumption. Administration ofLactobacillus paracaseitogether with maltodextrin decreased the number ofEscherichia colicolonising the jejunal mucosa of gnotobiotic piglets by 1 logarithm compared to the control group. Fructo-oligosaccharides (FOS) are naturally occurring oligosaccharides, mainly of plant origin.L. paracaseiadministered in combination with FOS significantly increased counts ofLactobacillusspp.,Bifidobacteriumspp., total anaerobes and total aerobes compared to the control group as well as theL. paracaseigroup. It also significantly decreasedClostridiumandEnterobacteriumcounts in the faeces of the weanling piglets compared with the control group. Dietary lipids influence the gastrointestinal microbiota and specifically the population of lactic acid bacteria. In gnotobiotic piglets the oral administration of an oil containing polyunsaturated fatty acids (PUFA) significantly increased the number ofL. paracaseiadhering to jejunal mucosa compared to the control group. Our results showed that maltodextrin KMS X-70 and PUFA can be used to enhance the effect of probiotic micro-organisms in the small intestine, and similarly FOS enhance the effect of probiotic micro-organisms in the large intestine.

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Proximate composition and fatty acid profile in different tissues of juvenile Siganus guttatus fed with Enteromorpha prolifera

Indian Journal of Animal Research ◽

10.18805/ijar.b-886 ◽

2018 ◽

Author(s):

C. Song ◽

F. Zhao ◽

J. Y. Liu ◽

Y. Wang ◽

X. R. Huang ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Polyunsaturated Fatty Acids ◽

Fatty Acid Profile ◽

Proximate Composition ◽

Nutritional Quality ◽

Total Content ◽

Enteromorpha Prolifera ◽

Health Function ◽

Different Tissues

The proximate composition and fatty acid profile of five tissues (head, skin, bones, muscle and liver) of juvenile Siganus guttatus fed with Enteromorpha prolifera were investigated. The results indicated that: (1) The content of protein and fat among these five tissues showed a significant differences (P Lass Than 0.05). The muscle has the highest content of protein and the lowest content of fat. (2) The highest content of fatty acid in SFA is 16:0 in all five tissues, the highest content of fatty acid in MUFA is18:1n-9c (cis) in skin, bones, muscle and liver, while the highest content of PUFA is DHA in muscle. Muscle contained the highest value of DHA and EPA, the total content was more than 100 times in bones. Muscle tissue is rich in many important polyunsaturated fatty acids such as EPA, DPA, DHA, ARA and ALA, which has better nutritional quality and immune and health function.

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N-3 Polyunsaturated Fatty Acids (PUFAs) Reverse the Impact of Early-Life Stress on the Gut Microbiota

PLoS ONE ◽

10.1371/journal.pone.0139721 ◽

2015 ◽

Vol 10 (10) ◽

pp. e0139721 ◽

Cited By ~ 81

Author(s):

Matteo M. Pusceddu ◽

Sahar El Aidy ◽

Fiona Crispie ◽

Orla O’Sullivan ◽

Paul Cotter ◽

...

Keyword(s):

Fatty Acids ◽

Gut Microbiota ◽

Polyunsaturated Fatty Acids ◽

Life Stress ◽

Early Life ◽

Early Life Stress ◽

The Impact

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Omega-3 polyunsaturated fatty acids-enriched hen eggs consumption enhances microvascular reactivity in young healthy individuals

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2017-0735 ◽

2018 ◽

Vol 43 (10) ◽

pp. 988-995 ◽

Cited By ~ 8

Author(s):

Ana Stupin ◽

Lidija Rasic ◽

Anita Matic ◽

Marko Stupin ◽

Zlata Kralik ◽

...

Keyword(s):

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Lipid Profile ◽

Serum Lipid ◽

Serum Lipid Profile ◽

Control Group ◽

Omega 3 ◽

Healthy Individuals ◽

Microvascular Reactivity ◽

Significant Difference

The beneficial effect of omega-3 polyunsaturated fatty acids (PUFA) supplementation on the cardiovascular (CV) system is well supported in CV patients; however, the effect of the consumption of omega-3 PUFA-enriched functional food in healthy individuals is still not fully elucidated. This study aimed to determine the effect of the consumption of omega-3 PUFA-enriched hen eggs on the microvascular reactivity (primary outcome), blood pressure (BP), and serum lipid profile in young healthy individuals. The control group (N = 16) ate 3 ordinary hen eggs (277 mg of omega-3 PUFAs/day), and the OMEGA-3 group (N = 20) ate 3 omega-3 PUFA-enriched eggs containing 259 mg of omega-3 PUFAs/egg daily (α-linolenic acid (ALA), 167 mg/egg; eicosapentaenoic acid (EPA), 7 mg/egg; docosahexaenoic acid (DHA), 84 mg/egg) for 3 weeks (777 mg of omega-3 PUFA/day). Postocclusive reactive hyperemia (PORH) in skin microcirculation assessed by laser Doppler flowmetry, serum lipid profile, fasting blood glucose, high-sensitivity C-reactive protein (hsCRP), and arterial BP were measured in all subjects before and after the protocol. PORH was significantly enhanced, and triglycerides, hsCRP, and BP were significantly decreased in the OMEGA-3 group compared with baseline measurements, whereas there was no significant difference in the control group after the protocol when compared with baseline. To the best of our knowledge, this is the first study to demonstrate that consumption of a mixture of omega-3 PUFA (ALA + EPA + DHA), provided via enriched hen eggs, elicits changes in the microvascular reactivity, BP, and triglyceride level in healthy subjects that are associated with CV benefits, thus suggesting that daily consumption of omega-3 PUFA-enriched eggs in healthy individuals may potentially contribute to CV risk factor attenuation and disease prevention.

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Studying the effects of fish oil infusion to prevent atrial fibrillation after cardiac surgery: data from implantable continuous cardiac monitor

Patologiya krovoobrashcheniya i kardiokhirurgiya ◽

10.21688/1681-3472-2014-3-12-18 ◽

2015 ◽

Vol 18 (3) ◽

pp. 12

Author(s):

S. M. Yefremov ◽

Ye. A. Pokushalov ◽

A. B. Romanov ◽

A. M. Chernyavskiy ◽

A. N. Shilova ◽

...

Keyword(s):

Atrial Fibrillation ◽

Fatty Acids ◽

Polyunsaturated Fatty Acids ◽

Postoperative Atrial Fibrillation ◽

Controlled Study ◽

Control Group ◽

Study Group ◽

Omega 3 ◽

Cardiac Monitor

The study was aimed at testing the hypothesis that perioperative infusion of omega-3 polyunsaturated fatty acids would reduce the incidence of postoperative atrial fibrillation in CAD patients operated under CPB. The authors carried out a prospective, randomised, double-blind, placebo-controlled study. 39 CAD patients who had undergone surgery under cardiopulmonary bypass were randomized into 2 groups. 18 patients were infused with omega-3 polyunsaturated fatty acids (Omegaven, Fresenius Kabi, Germany) starting with 200 mg/kg/day before anaesthesia induction for 24 hours followed by 100 mg/kg/day from second to seventh day postoperatively. 21 patients received an equivalent dose of placebo (Intralipid, Fresenius Kabi, Germany). The primary endpoint was freedom from atrial fibrillation at 2-year follow-up. A Reveal cardiac monitor was implanted subcutaneously in all patients. Data from cardiac monitor was collected on the 10th day and at 3, 6, 12 and 24 months postoperatively. Postoperative atrial fibrillation developed in 4 (19%) patients in the control group and in 5 (27.8%) patients in the study group at 10-day follow-up (p = 0.88). At 2-year follow-up, 5 (27.8%) patients in the control group and 6 (35.3%) patients in the study group had atrial fibrillation (p = 0.9). The results of our research do not confirm the efficiency of perioperative infusion of omega-3 polyun-saturated fatty acids to prevent the occurrence of atrial fibrillation.

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