scholarly journals Filter paper inhibits in vitro protocorm-like body formation in hybrid Cymbidium and reduces synseed germination, but buffers the negative impact of antibiotics

2014 ◽  
Vol 51 (1) ◽  
pp. 47-54
Author(s):  
JAIME A. TEIXEIRA DA SILVA
Keyword(s):  
Double Layer ◽  
Filter Paper ◽  
Negative Impact ◽  
Single Layer ◽  
Negative Effects ◽  
Cell Layers ◽  
Single Sheet ◽  
Plant Hybrid ◽  
The Impact

Abstract Only few studies in the plant tissue culture literature have examined the impact of filter paper on in vitro plant organogenesis. In this study, using a model plant, hybrid Cymbidium Twilight Moon ‘Day Light’, the impact of a single or double layer of Advantec #2 or Whatman #1 filter paper on new protocorm-like body (neo-PLB) formation on Teixeira Cymbidium (TC) medium was examined for half-PLBs (transgenic and non-transgenic), PLB-derived transverse thin cell layers (tTCLs), and PLB synseeds. In addition, the response of half-PLBs or tTCLs to two antibiotics (kanamycin and cefotaxime, commonly used in plant genetic transformation studies) was investigated either directly on gelled medium or on filter paper-overlaid medium. Filter paper negatively affected most growth and developmental parameters of all the explants tested, both transgenic and non-transgenic. A double sheet of filter paper had a significantly (P ≤ 0.05) more negative impact than a single sheet, relative to the control values (i.e., no filter paper). Kanamycin inhibited neo-PLB formation on TC medium, the negative impact being greater on a single layer than on a double layer of filter paper, i.e., filter paper buffered the growth-inhibiting characteristics of kanamycin. Up to 100 mg/l, cefotaxime showed no apparent negative effects on neo-PLBs formation and growth, although hyperhydricity was observed when filter paper was not used.

scholarly journals How Do Rare Earth Elements (Lanthanoids) Affect Root Development and Protocorm-Like Body Formation in Hybrid CYMBIDIUM?

2014 ◽  
Vol 22 (1) ◽  
pp. 67-75
Author(s):  
Jaime A. Teixeira da Silva
Keyword(s):  
Rare Earth ◽  
Rare Earth Elements ◽  
Negative Impact ◽  
Dry Weight ◽  
Nitrate Hexahydrate ◽  
Fresh Weight ◽  
Shoot Dry Weight ◽  
Plant Hybrid ◽  
The Impact

AbstractOnly few studies in the plant tissue culture literature have examined the impact of lanthanoids, or rare earth elements, on in vitro plant organogenesis. In this study, using a model plant, hybrid Cymbidium Twilight Moon ‘Day Light’, the impact of six lanthanoids (lanthanum (III) nitrate hexahydrate (La(NO3)3 · 6H2O), cerium (III) nitrate hexahydrate (Ce(NO3)3 · 6H2O), neodymium (III) nitrate hexahydrate (Nd(NO3)3 · 6H2O), praseodymium (III) nitrate hexahydrate (Pr(NO3)3 · 6H2O), samarium (III) nitrate hexahydrate (Sm(NO3)3 · 6H2O), gadolinium (III) nitrate hexahydrate (Gd(NO3)3 · 6H2O) on new protocorm-like body (neo-PLB) formation on Teixeira Cymbidium (TC) medium was examined. 0 (control), 1, 2, 4 and 8 mg·dm-3 of each lanthanoid was tested. All lanthanoids could produce more neo-PLBs and neo-PLB fresh weight than TC medium lacking plant growth regulators (PGRs), suggesting some PGR-like ability of lanthanoids, although PLB-related traits (percentage of half-PLBs forming neo-PLBs; number of neo-PLBs formed per half-PLB; fresh weight of half-PLB + neo-PLBs) was always significantly lower than TC medium containing PGRs. Except for Gd, all other lanthanoids had no negative impact on the number of new leaves from neo-PLB-derived shoots, but all lanthanoids showed a significantly lower plant height, shoot fresh weight and shoot dry weight and, in most cases, SPAD (chlorophyll content) value. In addition, using the same concentration of the six lanthanoids, the ability to fortify root formation of neo-PLB-derived plantlets was also assessed. Except for Sm, all other lanthanoids significantly increased the number of roots, root fresh and dry weight.

scholarly journals Evaluation of the Impact of Different Pain Medication and Proton Pump Inhibitors on the Osteogenic Differentiation Potential of hMSCs Using 99mTc-HDP Labelling

Life ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 339
Author(s):  
Tobias Grossner ◽  
Uwe Haberkorn ◽  
Tobias Gotterbarm
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Bone Metabolism ◽  
Negative Impact ◽  
Pain Medication ◽  
Group 3 ◽  
Group 5 ◽  
The Impact

First-line analgetic medication used in the field of musculoskeletal degenerative diseases, like Nonsteroidal anti-inflammatory drugs (NSAIDs), reduces pain and prostaglandin synthesis, whereby peptic ulcers are a severe adverse effect. Therefore, proton pump inhibitors (PPI) are frequently used as a concomitant medication to reduce this risk. However, the impact of NSAIDs or metamizole, in combination with PPIs, on bone metabolism is still unclear. Therefore, human mesenchymal stem cells (hMSCs) were cultured in monolayer cultures in 10 different groups for 21 days. New bone formation was induced as follows: Group 1 negative control group, group 2 osteogenic differentiation media (OSM), group 3 OSM with pantoprazole (PAN), group 4 OSM with ibuprofen (IBU), group 5 OSM with diclofenac (DIC), group 6 OSM with metamizole (MET), group 7 OSM with ibuprofen and pantoprazole (IBU + PAN), group 8 OSM with diclofenac and pantoprazole (DIC + PAN), group 9 OSM with metamizole and pantoprazole (MET + PAN) and group 10 OSM with diclofenac, metamizole and pantoprazole (DIC + MET + PAN). Hydroxyapatite content was evaluated using high-sensitive radioactive 99mTc-HDP labeling. Within this study, no evidence was found that the common analgetic medication, using NSAIDs alone or in combination with pantoprazole and/or metamizole, has any negative impact on the osteogenic differentiation of mesenchymal stem cells in vitro. To the contrary, the statistical results indicate that pantoprazole alone (group 3 (PAN) (p = 0.016)) or diclofenac alone (group 5 (DIC) (p = 0.008)) enhances the deposition of minerals by hMSCS in vitro. There is an ongoing discussion between clinicians in the field of orthopaedics and traumatology as to whether post-surgical (pain) medication has a negative impact on bone healing. This is the first hMSC in vitro study that investigates the effects of pain medication in combination with PPIs on bone metabolism. Our in vitro data indicates that the assumed negative impact on bone metabolism is subsidiary. These findings substantiate the thesis that, in clinical medicine, the patient can receive every pain medication needed, whether or not in combination with PPIs, without any negative effects for the osteo-regenerative potential.

scholarly journals In the Same Boat, but not Equals: The Heterogeneous Effects of Indirect Taxation on Child Health in Punjab-Pakistan

2020 ◽  
Vol 11 (3) ◽  
Author(s):  
Faiz Ur Rehman ◽  
Muhammad Nasir
Keyword(s):  
Negative Impact ◽  
Income Group ◽  
Z Score ◽  
Negative Effects ◽  
Indirect Taxation ◽  
Nutrition Survey ◽  
Lower Income Group ◽  
Food Items ◽  
Heterogeneous Effects ◽  
The Impact

AbstractThe 2011 National Nutrition Survey of Pakistan revealed that 51% of the country’s population was consuming less than 2,100 calories a day. In the backdrop of rising food insecurity, hunger, and malnutrition in Pakistan, this study aims to measure the effects of indirect taxation on health outcomes of children (<5 years). More specifically, the impact of the incidence of General Sales Tax (GST) in the province of Punjab has been estimated on a child’s height and weight. The proponents of the uniform GST argue that the tax would not affect children because most food items consumed by children are exempted from the GST. However, the opponents believe that households, especially those belonging to the lower-income group, would reallocate resources away from children in the face of higher GST. To study these effects, we utilized three different waves (2007–08, 2011 & 2014) of Multiple Indicators Cluster Surveys (MICS). The results show that the tax incidence, and not the GST rate, has a significantly negative impact on children’s height-for-age Z-score (HAZ). No effect was found on the weight-for-age-z-score (WAZ). These results are robust to different specifications and exhibit considerable heterogeneity across different income groups. These findings suggest that the exemption of certain food items for children from the GST may not eliminate the negative effects of this tax on a child’s health. Thus, our study raises concerns about the long term welfare consequences of GST.

scholarly journals PFAS Environmental Pollution and Antioxidant Responses: An Overview of the Impact on Human Field

2020 ◽  
Author(s):  
Marco Bonato ◽  
Francesca Corrà ◽  
Marta Bellio ◽  
Laura Guidolin ◽  
Laura Tallandini ◽  
...  
Keyword(s):  
Environmental Pollution ◽  
Human Health ◽  
Antioxidant Defense System ◽  
Epidemiological Studies ◽  
Negative Effects ◽  
Environmental Distribution ◽  
Commercial Applications ◽  
The Impact

Due to their unique properties, perfluorinated substances (PFAS) are widely used in multiple industrial and commercial applications, but they are toxic for animals, humans included. This review presents some available data on the PFAS environmental distribution in the world, and in particular in Europe and in Veneto region of Italy, where it has become a serious problem for human health. The consumption of contaminated food and drinking water is considered one of the major source of exposure for humans. Worldwide epidemiological studies report the negative effects that PFAS have on human health, due to environmental pollution, including infertility, steroid hormone perturbation, thyroid, liver and kidney disorders and metabolic disfunctions. In vitro and in vivo researches correlated PFAS exposure to oxidative stress effects (in mammals as well as in other vertebrates of human interest), produced by a PFAS-induced increase of reactive oxygen species formation. The cellular antioxidant defense system is activated by PFAS, but it is only partially able to avoid the oxidative damage to biomolecules.

The Impact of Ceta Programs on Components of Participants' Earnings

ILR Review ◽  
1987 ◽  
Vol 40 (3) ◽  
pp. 430-441 ◽  
Author(s):  
Katherine P. Dickinson ◽  
Terry R. Johnson ◽  
Richard W. West
Keyword(s):  
Nearest Neighbor ◽  
Negative Impact ◽  
Positive Impact ◽  
Hourly Wage ◽  
Negative Effects ◽  
Hours Worked ◽  
Positive Effects ◽  
Hourly Wage Rate ◽  
Women's Earnings ◽  
The Impact

This paper provides the first estimates of the net impact of CETA participation on the components of CETA participants' post-program earnings. Employing a sample of 1975 CETA enrollees and comparison groups drawn from the March 1978 CPS using a nearest-neighbor matching technique, the authors estimate statistically significant negative effects on men's earnings and statistically significant positive effects on women's earnings. These results stem partly from the impact of CETA participation on the likelihood of being employed after leaving the program (negative for men, positive for women), but also from a negative impact on hours worked during the year and hourly wage rate for men and a large positive impact on hours worked per week and weeks worked per year for women.

STUDY ON EFFECTS OF DISTRUTIVE AGENTS ON THE ARTWORKS

2017 ◽  
Vol 31 (1) ◽  
pp. 75-81
Author(s):  
Valeria Mirela Brezoczki ◽  
◽  
Emese Bonta ◽  
Keyword(s):  
Negative Impact ◽  
Art Museum ◽  
External Factors ◽  
Cultural Value ◽  
Negative Effects ◽  
Active Monitoring ◽  
Baia Mare ◽  
Different Types ◽  
The Impact ◽  
Over Time

The paper describes a series of effects created by the impact of environmental factors on artworks in museums, as well as the way that active monitoring of these destructive agents (temperature and relative humidity) is done. Over time, artefacts exhibited within museums are subject to a series of degradations caused by external factors (air components, humidity, temperature, sunlight, bacteria, molds or fungi etc.), which can leave a negative impact on these goods with cultural value. The main observed negative effects are directly and intimate related to the deterioration of wood sculptures by the occurrence of cracks and the installation of different types of bacteria; the appearance of brownish-red spots on the surface of the paper and the increase in its reliability; various types of corrosion of artworks from different metals; color losses and cracks on paintings etc. The study brings to the fore the damaging effects produced on the different cultural works hosted within the County Art Museum - Art Center Baia Mare.

Economy of households under conditions of pandemic instability

2021 ◽  
pp. 98-109
Author(s):  
Mariana Fedyk
Keyword(s):  
Negative Impact ◽  
Alcoholic Beverages ◽  
Baseline Scenario ◽  
Household Economy ◽  
Negative Effects ◽  
Subsistence Level ◽  
Goods And Services ◽  
Academic Paper ◽  
Absolute Criterion ◽  
The Impact

The purpose of the academic paper lies in assessing the state of income, expenditure and savings of households under conditions of pandemic instability. The research methodology is based on the statistical analysis of data on income, resources and savings of households in Ukraine for 2010-2020. The scientific novelty involves identifying the positive and negative effects of the pandemic on the household economy. Conclusions. The positive and negative effects of the impact of spreading the pandemic on the household economy have been revealed in the research, and as a result, the decline in economic activity and the growth of unemployment in Ukraine. It has been determined that in the period of 2020 pandemic, costs decreased by 2% and resources increased by 3%. In 2020, the share of expenditures on food and non-alcoholic beverages increased from 46,6% to 48,1% in 2019, and on non-food goods and services - decreased from 41,5% to 39,8%. The population with per capita equivalent total income per month, below the actual subsistence level, was 8,9 million people in 2019 (23,1%), in 2020 – 8,8 million people (23,2%). Despite experts’ assessments of the likely increase in poverty as a result of COVID-19 spreading under two scenarios (according to the absolute criterion, it will increase from 27,2 to 43,6%; according to an absolute criterion, it will increase from 27,2 to 50,8%). It can be noted that the COVID-19 pandemic has had a negative impact on the poorest sections of the population with low incomes. It has been determined that in the context of social-economic impact of COVID-19, families who find it more difficult to diversify their own incomes are the most vulnerable ones. The following categories have been most affected by the pandemic (they will have had the largest increase in poverty compared to the baseline scenario), namely: households with three or more children; single parents with children; households with children under three; single retirees over 65 years.Along with this, thanks to the monetary policy that has ensured a stable level of inflation and return on deposits, the share of household deposits has increased the most in the last ten years to 27%. However, it is worth noting that quarantine and the COVID-19 pandemic have led to a trend towards a partial flow of population resources from time deposits to card and savings accounts. After all, citizens sought to have free money in case of unforeseen expenses due to the uncertainty of the future development of events. Key words: households, income, resources, consumer expenditures, economic crisis, pandemic instability, quarantine restrictions.

The Impact of Corruption on Entrepreneurship

2015 ◽  
pp. 1-13
Author(s):  
Gil Avnimelech ◽  
Yaron Zelekha
Keyword(s):  
Developing Countries ◽  
Literature Review ◽  
Negative Impact ◽  
Developed Countries ◽  
Entrepreneurial Activity ◽  
Negative Effects ◽  
Societal Costs ◽  
Present Evidence ◽  
Growing Body ◽  
The Impact

There is a consensus that corruption may result in high societal costs. A growing body of research reveals the negative effects of corruption on a variety of economic indicators. This chapter presents a literature review on the impact of corruption on entrepreneurship. It allows us to suggest that one of the transition channels through which corruption has impacted growth is entrepreneurship. The main channels in which corruption impacts entrepreneurship is through reduced incentives for entrepreneurial activity and reduced trust within the system. The authors present evidence that the negative impact of an incremental increase in the level of corruption on entrepreneurship is more harmful in developed countries than in developing countries. Thus, they stress the need for more research in this area with the aim of establishing appropriate frameworks for the fight of corruption in both developing and developed countries and suggest significant gains from anti-corruption efforts, especially in developed countries.

scholarly journals Social Vulnerability, Gender and Disasters. The Case of Haiti in 2010

2020 ◽  
Vol 12 (9) ◽  
pp. 3574 ◽  
Author(s):  
Mar Llorente-Marrón ◽  
Montserrat Díaz-Fernández ◽  
Paz Méndez-Rodríguez ◽  
Rosario González Arias
Keyword(s):  
Natural Disasters ◽  
International Development ◽  
Social Vulnerability ◽  
Negative Impact ◽  
Research Work ◽  
The United States ◽  
Negative Effects ◽  
Unequal Distribution ◽  
And Gender ◽  
The Impact

The study of vulnerability constitutes a central axis in research work on sustainability. Social vulnerability (SV) analyzes differences in human capacity to prepare, respond and recover from the impact of a natural hazard. Although disasters threaten all the people who suffer from them, they do not affect all members of society in the same way. Social and economic inequalities make certain groups more vulnerable. Factors such as age, sex, social class and ethnic identity increase vulnerability to a natural disaster. Ten years after the earthquake in Haiti in 2010, this work deepens the relationship between natural disasters, SV and gender, exploring the unequal distribution of the SV in the face of a seismic risk. The source of statistical information has been obtained from the Demographic and Health Survey (DHS), developed by the United States Agency for International Development (USAID). Multicriteria decision techniques (TOPSIS) and the differences in differences (DID) technique are used to analyze variations in gender inequality in SV as a result of the catastrophic event. The results obtained reinforce the idea of the negative impact of the disaster on the SV. Additionally, an intensification of the negative effects is observed when the household is headed by a woman, increasing the gap in SV between households headed by women and the rest of the households. The conclusions obtained show additional evidence of the negative effects caused by natural disasters on women, and important implications for disaster risk management are derived that should not be ignored.

scholarly journals Embryotoxic impact of Zika virus in a rhesus macaque in vitro implantation model†

2020 ◽  
Vol 102 (4) ◽  
pp. 806-816 ◽  
Author(s):  
Lindsey N Block ◽  
Matthew T Aliota ◽  
Thomas C Friedrich ◽  
Michele L Schotzko ◽  
Katherine D Mean ◽  
...  
Keyword(s):  
Rhesus Macaque ◽  
Pregnancy Outcomes ◽  
Zika Virus ◽  
Negative Impact ◽  
Culture Media ◽  
Blastocyst Formation ◽  
Placental Development ◽  
Zikv Infection ◽  
The Impact

Abstract Zika virus (ZIKV) infection is associated with adverse pregnancy outcomes in humans, and infection in the first trimester can lead to miscarriage and stillbirth. Vertical and sexual transmissions of ZIKV have been demonstrated, yet the impact of infection during the initial stages of pregnancy remains unexplored. Here we defined the impact of ZIKV on early embryonic and placental development with a rhesus macaque model. During in vitro fertilization (IVF), macaque gametes were inoculated with a physiologically relevant dose of 5.48log10 plaque-forming units (PFU) of Zika virus/H.sapiens-tc/PUR/2015/PRVABC59_v3c2. Exposure at fertilization did not alter blastocyst formation rates compared to controls. To determine the impact of ZIKV exposure at implantation, hatched blastocysts were incubated with 3.26log10, 4.26log10, or 5.26log10 PFU, or not exposed to ZIKV, followed by extended embryo culture for 10 days. ZIKV exposure negatively impacted attachment, growth, and survival in comparison to controls, with exposure to 5.26log10 PFU ZIKV resulting in embryonic degeneration by day 2. Embryonic secretion of pregnancy hormones was lower in ZIKV-exposed embryos. Increasing levels of infectious virus were detected in the culture media post-exposure, suggesting that the trophectoderm is susceptible to productive ZIKV infection. These results demonstrate that ZIKV exposure severely impacts the zona-free blastocyst, whereas exposure at the time of fertilization does not hinder blastocyst formation. Overall, early stages of pregnancy may be profoundly sensitive to infection and pregnancy loss, and the negative impact of ZIKV infection on pregnancy outcomes may be underestimated.

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