scholarly journals Comparative Efficacy of Silymarin and Choline Chloride (Liver Tonics) in Preventing the Effects of Aflatoxin B1 in Bovine Calves

2016 ◽  
Vol 19 (3) ◽  
pp. 545-551 ◽  
Author(s):  
O. Naseer ◽  
J.A. Khan ◽  
M.S. Khan ◽  
M.O. Omer ◽  
G.A. Chishti ◽  
...  

Abstract Aflatoxins are secondary metabolites produced by Aspergillus spp. which are injurious to animals and humans The aim of this study was to determine the effects of aflatoxin B1 (AFB1) on Average Daily Feed Intake (ADFI), Average Daily Weight Gain (ADWG), haematological and serum biochemical responses of Bovine Calves and to determine the comparative efficacy of two different liver tonics against AFB1. Twenty seven calves were selected from herd and divided into 3 groups. All calves were fed with 1.0 mg/kg AFB1 for a period of 10 days. After that they were fed with liver tonics: Silymarin fed at a rate of 600 mg/kg and Choline chloride 500 mg/kg for 7 days. The results indicate that the ADFI and ADWG of AFB1 treated calves decreased significantly. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine significantly increased due to AFB1. In haematology the total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin concentration (HGB), haematocrit levels (HCT), mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV) and mean corpuscular haemoglobin concentration (MCHC), lymphocyte %, neutrophil % and monocyte % significantly decreased in AFB1 treated calves after 10 days of feeding. Both liver tonics significantly (p<0.05) improved all the parameters, including ADFI, ADWG, hematologial and serum biochemical test. However, Silymarin comparatively more efficiently ameliorate the effects induced by AFB1 than choline chloride.

Author(s):  
Y. Garba ◽  
E. A. Adeola

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.


2020 ◽  
Vol 47 (1) ◽  
pp. 65-80
Author(s):  
J. O. Arogbodo ◽  
I. B. Osho ◽  
O. B. Faluyi ◽  
T. A. M. Awoniyi

This experiment was conducted for eight weeks to evaluate the haematological and serum biochemical indices of broiler chickens infected with Salmonella Gallinarum (Gr. D1-1, 9, 12) and treated with ethanolic leaf extract of Chrysophyllum albidum (G. Don). The experimental design was a factorial arrangement in a completely randomised design (CRD) involving one hundred and sixty two (162) unsexed day-old Arbor Acre broiler chicks. The chickens were allotted to nine (9) groups of three (3) replicates with each replicate comprising six (6) birds of infected, non-infected, treated and untreated group. Phytochemical screening of C. albidum revealed the presence of tannins, saponins, cardiac glycosides, steroids, terpenoids and flavonoids. The extract and the standard drug were th administered at 7 day post infection. There were significant differences (P< 0.05) in Erythrocyte Sedimentation Rate (ESR), Packed Cell Volume (PCV), Red Blood Cell (RBC), Haemoglobin (Hb), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Volume (MCV), Lymphocyte (LYM) and none in Heterophil (HET), Mean Corpuscular Haemoglobin Concentration (MCHC), Monocytes, Basophils and Eosinophils at all levels. The highest values of PCV (33.67±1.86a ), RBC (3.02±0.4a ) and Hb (11.23±0.62a ) though normal were observed in the standard drug (Doxygen®) group. Most of the serum biochemical parameters were found to be within range usually seen in avian species. It was concluded that ethanolic leaf extract of C. albidum had no deleterious effect on haematological and biochemical indices of broiler chickens and hence can be further explored pharmaceutically for its usefulness in treating fowl typhoid.


2020 ◽  
Vol 7 (1) ◽  
pp. 113-120
Author(s):  
Md Shohag ◽  
Mohammad Raguib Munif ◽  
Mst Nargis Jahan ◽  
Md Mizanur Rahman ◽  
Md Rafiqul Alam

Haematobiochemical changes of ovine (sheep) blood were investigated during preservation and storage with Citrate Phosphate Dextrose Adenine-1 (CPDA-1) and Acid Citrate Dextrose (ACD) for transfusion. Twelve healthy sheep were selected and divided into two equal groups: group X (n=6) and group Y (n=6). Thirty-five ml of blood was collected from each animal and preserved with CPDA-1 in group X and ACD in group Y under 4°C in refrigerator for 28 days. Haematological changes viz., total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC); and biochemical changes viz., total protein (TP) and pH were evaluated immediately after blood collection and thereafter on day-1, day-3, day-7, day-14, day-21 and day-28 for both groups. In ACD preserved blood; TEC, TLC, Hb and PCV decreased significantly (P<0.01) from day-14 onward, whereas in CPDA-1 preserved blood, these parameters decreased significantly (P<0.01) from day-21 onward. Blood preserved in ACD showed significant changes (P<0.01) in MCV, MCH and MCHC respectively from day- 7, day-14 and day-21 onward, whereas blood preserved in CPDA-1 showed no significant changes in the same parameters throughout the experiment. In both groups, no significant changes were noticed in TP but significant changes (P<0.01) were observed in pH with the progression of storage period. These findings elicited that both ACD and CPDA-1 exert certain haematobiochemical changes in stored sheep blood, however, CPDA-1 was more efficient than ACD in terms of maintaining proper levels of TEC, TLC, Hb., PCV, MCV, MCH and MCHC during preservation and storage of sheep blood for transfusion. Res. Agric., Livest. Fish.7(1): 113-120, April 2020


2014 ◽  
Vol 59 (No. 12) ◽  
pp. 564-570 ◽  
Author(s):  
E. Straková ◽  
K. Karásková ◽  
D. Zapletal ◽  
P. Suchý

The effect of melamine and cyanuric acid contaminated diets on basic haematological and biochemical blood indicators in male broiler chickens (Ross 308) was studied. The chickens were divided into 6&nbsp;experimental groups (30 birds per group) and fed diets with an addition of 50 or 100 mg of melamine or 50 or 100 mg of cyanuric acid per kg of feed, with the contaminants added separately (either melamine or cyanuric acid) or in combination (melamine + cyanuric acid). The control group (C) was fed a diet without melamine or cyanuric acid. At the end of the experiment (day 40), 8 birds per treatment group were randomly selected for haematological and biochemical examination of blood. Red blood cell count, haemoglobin concentration, haematocrit value, mean corpuscular haemoglobin concentration, mean corpuscular haemoglobin, mean cell volume, and total leukocyte count did not differ significantly among the respective groups of broiler chickens (P &gt; 0.05). The concentrations of melamine and cyanuric acid used in the contaminated diet for broiler chickens led to highly significant changes in the content of total protein (TP), glucose (Glu), Ca, P, Na, and K (P &lt; 0.01) and to significant changes in the content of Mg (P &lt; 0.05) in their blood plasma. In comparison to the C&nbsp;group, the decrease in the content of TP, Glu, Ca, P, Na, K, and Mg in blood plasma was most pronounced in broilers fed the diets contaminated with cyanuric acid only or diets with the simultaneous contamination with melamine and cyanuric acid. On the basis of our results, it can be concluded that the contamination of feed with melamine and cyanuric acid, separately or in combination, results in impaired renal function and probably also in partial liver damage. &nbsp;


2014 ◽  
Vol 7 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Sunita Pande ◽  
Prabhu Narain Saxena ◽  
Brijender Bhushan ◽  
Nishi Saxena

Abstract Pyrethroids, commercially available pesticides, are greatly in use these days, and thus they carry considerable chances of contaminating various ecosystems. Haematotoxicity of cypermethrin, a broadly used type II pyrethroid, has been assessed in the present study. Selected parameters included determination of total RBC count, haemoglobin concentration (Hb conc.), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), erythrocyte sedimentation rate (ESR), total leukocyte count (TLC), differential leukocyte count (DLC), along with qualitative analysis of blood and bone marrow. Of these parameters, those showing significant decline following cypermethrin intoxication included total RBC count, Hb conc., PCV, MCV, MCH, whereas non-significant decrease was observed in the case of MCHC. ESR, TLC and DLC, on the other hand, increased significantly following cypermethrin intoxication. Qualitative changes included altered red cell morphology such as microcystosis, appearance of stomatocytes, poikilocytosis, giant platelet formation, etc. in peripheral blood and increased erythroid precursors in bone marrow of treated rats. These parameters were however normalised following twenty-two days of recovery phase


2021 ◽  
Vol 48 (1) ◽  
pp. 197-206
Author(s):  
P. S Kolo ◽  
B. Otu ◽  
A. A. Banjo ◽  
H. N. Kolo

Wattle is of utmost ornamental importance for courting potential mates and influencing thermoregulatory mechanisms which help the animal adapt to the environment. It also provides information on relationship between haematological and serum biochemical parameters. A study on haematology and serum biochemistry of wattled and non wattled Red Sokoto does and their offspring was carried out at the Teaching and Research Farm of the Department of Animal Production, Federal University of Technology, Minna. Fifty (52) Red Sokoto goats comprising of thirty-two (32) does four (4) bucks and sixteen (16) weaned kids managed semi-intensively were used for the study Blood samples were collected using 5 ml syringe and 22-guage needle from the jugular vein. 5 ml of blood was collected from each goat used out of which 2.5 ml was dispensed into Ethylene Diamine Tetra Acetic Acid (EDTA) bottle while the remaining 2.5 ml was dispensed into plain (anticoagulant free) bottles and labelled properly according to the treatment group. Data collected were analyzed using SAS statistical package. It was observed: that wattle had significant effect (p<0.05) on Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), White Blood Cell (WBC), Sodium, Potassium, Calcium, Chloride, Phosphorus, Cholesterol, Total Protein, Low Density Lipoprotein (LDL) and Total Bilirubin of Red Sokoto Does but had no significant influence on the haematology and serum biochemistry of wean Red Sokoto kids. Does in T3 had the highest MCH values of 63.50 mmo/l while treatments T1, T2 and T4 had values of 23.00 mmo/l, 33.00mmo/l and 34.00mmo/l respectively. Also Does in T2, T3 and T4 recorded higher calcium levels of 2.54mmo/l, 2.56mmol/l and 2.61mmo/l) respectively compared to values of 2.29mmo/l recorded in T1. These relevant influence of wattle therefore should suggest the deployment of deliberate effort to preserve the wattle gene to prevent the goats carrying the gene from going to extinction.     L'acacia est de la plus haute importance ornementale pour courtiser les partenaires potentiels et influencer les mécanismes de thermorégulation qui aident l'animal à s'adapter à l'environnement. Il fournit également des informations sur la relation entre les paramètres hématologiques et biochimiques sériques. Une étude sur l'hématologie et la biochimie sérique des femelles de chèvres Sokoto femelle rouge et de leur progéniture a été réalisée à la Ferme d'enseignement et de recherche du Département de la production animale, Université fédérale de technologie, Minna. Cinquante (52) chèvres Sokoto femelle rouges comprenant trente-deux (32) femelles quatre (4) mâles et seize (16) chevreaux sevrés gérés de manière semi-intensive ont été utilisés pour l'étude. Des échantillons de sang ont été prélevés à l'aide d'une seringue de 5 ml et d'une aiguille de calibre 22 de la veine jugulaire. 5 ml de sang ont été collectés sur chaque chèvre utilisée, dont 2,5 ml ont été distribués dans un flacon d'acide éthylène diamine tétra acétique (le 'EDTA') tandis que les 2,5 ml restants ont été distribués dans des flacons simples (sans anticoagulant) et étiquetés correctement selon le groupe de traitement. Les données collectées ont été analysées à l'aide du progiciel statistique 'SAS'. Il a été observé : que l'acacia avait un effet significatif (p <0,05) sur l'hémoglobine corpusculaire moyenne (le 'MCH'), la concentration moyenne d'hémoglobine corpusculaire (le 'MCHC'), les globules blancs (GB), le sodium, le potassium, le calcium, le chlorure, le phosphore, le cholestérol, Protéine, lipoprotéine de faible densité (LDL) et bilirubine totale de chèvres Sokoto femelle rouges mais n'ont eu aucune influence significative sur l'hématologie et la biochimie sérique des enfants sevrés Red Sokoto. Les lapins de T3 avaient les valeurs 'MCH' les plus élevées de 63,50 mmo / l tandis que les traitements T1, T2 et T4 avaient des valeurs de 23,00 mmo / l, 33,00 mmo / l et 34,00 mmo / l respectivement. En T2, T3 et T4 ont également enregistré des taux de calcium plus élevés de 2,54 mmo / l, 2,56 mmol / l et 2,61 mmo / l) respectivement par rapport aux valeurs de 2,29 mmo / l enregistrées en T1. Ces influences pertinentes de l'acacia devraient donc suggérer le déploiement d'efforts délibérés pour préserver le gène de l'acacia afin d'éviter que les chèvres porteuses du gène ne s'éteignent.


2006 ◽  
Vol 75 (4) ◽  
pp. 561-569 ◽  
Author(s):  
H. Kroupová ◽  
J. Máchová ◽  
V. Piačková ◽  
M. Flajšhans ◽  
Z. Svobodová ◽  
...  

Common carp (Cyprinus carpio L.) were exposed to nitrite (1.45 mmol l-1 NO2-) for 48 hours at 14 °C and 20 °C, in order to investigate the mechanism of nitrite poisoning at these water temperatures. The effect of nitrite exposure on fish was assessed on selected haematological and biochemical indicators of the blood. Moreover, nitrite accumulation in the blood, liver and muscle was measured. Nitrite exposure produced high levels of methaemoglobin (88.2 ± 3.3% and 92.9 ± 6.1%) at both water temperatures compared with controls (0.3 ± 0.6% and 2.6 ± 3.0%). High fish mortality occurred in experimental groups (30% and 51%) compared with controls (0%). Nitrite exposure also resulted in an accumulation of nitrite in the fish body. The highest nitrite levels developed in the blood plasma, followed by the liver and muscle, respectively. Carp concentrated nitrite in the blood plasma and tissues to markedly higher levels at higher temperature (20 °C). The plasma nitrite concentrations (10.5 ± 1.9 mmol l-1) were in this case more than 7 times higher than the environmental one. At lower temperature (14 °C), plasma nitrite concentration reached 5.0 ± 1.5 mmol l-1. In either event, plasma K+ levels increased and Cl- levels and osmolality remained unchanged. Plasma Na+ levels slightly decreased at the higher temperature. Nitriteexposed fish showed lower haematocrit values (PCV) at both experimental temperatures compared with controls. At 20 °C, the blood haematocrit decrease (0.20 ± 0.02 l l-1) was accompanied by a low erythrocyte count (1.05 ± 0.12 1012 l-1) and by a low haemoglobin level (51 ± 11 g l-1). At the lower temperature (14 °C), the haematocrit decrease (0.25 ± 0.02 l l-1) was caused by a low mean corpuscular volume (167 ± 27 fl). No significant changes were observed in the mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), or selected erythrocyte dimensions (major axis, minor axis and aspect ratio).


2021 ◽  
Author(s):  
Irena Golinar Oven ◽  
Alenka Nemec Svete ◽  
Melita Hajdinjak ◽  
Jan Plut ◽  
Marina Stukelj

Abstract BackgroundHaematological examination is an important diagnostic tool in the assessment of pig health status. The present study aimed to assess haematological parameters in pigs of different age categories from six farrow-to-finish farms differing in herd health status. The following pig categories were included: 5 age groups of growers (5, 7, 9–10, 11 and 12–13 weeks-old), fatteners and breeding pregnant sows. Individual blood samples for determining complete blood count and white blood cell differential count were taken and group samples of oral fluid and faeces were collected from each animal category in each of the six farms and tested for the detection of Porcine Circovirus Type 2 (PCV2), Porcine Reproductive and Respiratory Virus (PRRSV), and Hepatitis E Virus (HEV) using PCR, RT-PCR, and qRT-PCR protocols. Individual blood samples were analysed using an automated laser-based haematology analyser. The following haematological parameters were reported: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet count (PLT), and percentage and number of neutrophils, lymphocytes, monocytes, eosinophils, basophils, and "large unstained cells" - LUCs. ResultsOn farms free of PRRSV, PCV2 and HEV, age had significant effect on the following parameters: WBC, Hb, MCH, MCHC, PLT, percentage of neutrophils, lymphocytes and eosinophils and absolute numbers of neutrophils, lymphocytes, monocytes, basophils and LUCs. On farms with PRRS, PCV2 and/or HEV, age significantly affected all observed blood parameters except the percentage of LUCs. The percentages of lymphocytes, MCV and Hct were significantly lower by PRRSV while WBC, PLT, percentage and absolute number of neutrophils, basophils and LUCs increased. Significantly lower percentages of lymphocytes and increased percentages and absolute numbers of neutrophils, eosinophils and basophils were caused by PCV2 presence. Significantly lower percentage of lymphocytes and MCV and increased RBC, Hb, percentage and number of basophils and percentage of neutrophils were caused by HEV. Conclusions Alterations of haematological parameters reflected the health status of pigs of different categories on infected and on non-infected farms. Age-related changes in haematological parameters occurred in clinically healthy and in infected pigs.


2021 ◽  
Vol 36 (1) ◽  
pp. 13-21
Author(s):  
A.B. Ella ◽  
E.T. Azua ◽  
C.U. Aguoru ◽  
A.A. Onekutu ◽  
F.A. Ella

The study evaluated sub-lethal toxicity of commercial glyphosate-based Di-ammonium formulation commonly known as ‘Touchdown’ on haematological profile of Clarias gariepinus juveniles. A total of two hundred catfish juveniles of mean weight 31.07±1.23g and mean length 19.50 ± 0.50cm were exposed to different  concentrations of Di-ammonium formulation (1.26, 2.52, 5.03 and 0.00 mg/l) for eight (8) weeks. Each concentration was treated in triplicate using a static bioassay system. Physico-chemical parameters of the test water were monitored throughout the study period. Results showed that haematocrit (HCT), red blood cell (RBC), haemoglobin (HGB), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC) and Platelets (PLT) counts decreased significantly (p<0.05) with increase in concentrations of Touchdown. Whereas, white blood cell (WBC) counts increased significantly (p<0.05) with increasing concentrations of the glyphosate-based formulation. Water temperature, pH, Total dissolved solid (TDS) and electrical conductivity (EC) increased linearly with increasing concentration, while dissolved oxygen content decreased significantly (p<0.05). The results from this study indicated that Di-ammonium glyphosate–based formulation had negative consequences on the blood of treated fish. In view of this, the application of Di-ammonium glyphosate-based formulation should be done in a sustainable manner with proper regulations.


2017 ◽  
Vol 9 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Onyinyechukwu A. AGINA ◽  
Wilfred S. EZEMA ◽  
Ezinwa M. IWUOHA

The present study established the reference values and sex differences in the erythrocytic and serum biochemistry parameters of domestic adult quails (Coturnix coturnix). Ninety five adult birds, comprising of 42 males and 53 female Japanese quails were sampled using a simple random sampling technique. Standard procedures were carried out in all haematology and serum biochemistry determinations. The overall mean for the erythrocytic and serum biochemistry parameters were as follows: packed cell volume (PCV) 43.11%, red blood cell count (RBC) 4.31 × 106/µl, haemoglobin concentration (Hbc) 16.21 g/dl, mean corpuscular volume (MCV) 100.69 fl, mean corpuscular haemoglobin (MCH) 39.17 pg, mean corpuscular haemoglobin concentration (MCHC) 39.35 g/dl, aspartate aminotransferase (AST) 59.99 IU/L, alanine aminotransferase (ALT) 20.85 IU/L, alkaline phosphatase (ALP) 107.54 IU/L, total proteins (TP) 5.19 g/dl, albumin (ALB) 3.25 g/dl, globulin (GLB) 1.94 g/dl, albumin: globulin 1.73, total cholesterol (TCHOL) 146.69 mg/dl, total bilirubin (TBIL) 2.37 mg/dl, uric acid (UA) 16.02 mg/dl and creatinine (CREAT) 0.44 mg/dl. The PCV of the males were significantly higher than that of the females, while the MCH and MCHC of the females were significantly higher than those of the males. The serum total proteins, albumin, globulin, uric acid, creatinine, and total cholesterol values of the female quails were higher than those of the male quails. The present data might be useful to avian specialists and veterinary clinicians, but more research works should be carried out on quails to increase the information data base, especially in the tropics.


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