The effects of hydraulic calcium silicate containing endodontic materials on oxidative stress in erythrocytes and liver

2017 ◽  
Vol 43 (3) ◽  
pp. 333-341 ◽  
Author(s):  
Kadriye Demirkaya ◽  
Birsen Can Demirdöğen ◽  
Zeynep Öncel Torun ◽  
Onur Erdem ◽  
Yaşar Meriç Tunca
Keyword(s):  
Oxidative Stress ◽  
Calcium Silicate ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Thiobarbituric Acid Reactive Substances ◽  
Dental Cement ◽  
Oxidative Stress Parameters ◽  
Liver Tbars ◽  
Stress Parameters

Abstract Objective: The aim of this study was to evaluate the effects of hydraulic calcium silicate endodontic cements, MTA Angelus, MTA Fillapex, and Theracal LC, on erythrocyte and liver oxidative stress parameters of rats. Methods: Right upper incisor of each rat was extracted and polyethylene tubes containing the dental cements, or left empty for the control group, were inserted into the extraction socket. Blood and liver samples of each animal were obtained after 7, 30, or 60 days. Thiobarbituric acid reactive substances (TBARS) levels and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities were determined by spectrophotometry. Results: Erythrocyte and liver TBARS levels, and CAT and SOD enzymatic activities were significantly increased in dental cement applied groups compared with controls on day 7. The highest erythrocyte and liver TBARS concentrations were observed in the MTA Angelus group on day 7 of exposure. On day 30, erythrocyte CAT activity remained markedly high, but the other parameters returned to almost normal levels. On day 60, all parameters were similar between the control and the experimental groups. Conclusions: This is the first study to show that TBARS levels and antioxidant enzyme activities are transiently increased as a result of dental cement application.

Association Between Magnesium and Oxidative Stress in Patients with Obesity

2020 ◽  
Vol 16 (5) ◽  
pp. 743-748
Author(s):  
Ana R.S. de Oliveira ◽  
Kyria J.C. Cruz ◽  
Jennifer B.S. Morais ◽  
Juliana S. Severo ◽  
Jéssica B. Beserra ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Magnesium Concentration ◽  
Control Group ◽  
Compensatory Mechanism ◽  
Thiobarbituric Acid Reactive Substances ◽  
Magnesium Intake ◽  
Significant Difference ◽  
Dietary Magnesium ◽  
And Control

Background: The role of minerals in preventing the generation of oxidative stress in obese individuals has been evaluated. Magnesium is an antioxidant nutrient and a cofactor of enzymes involved in the cell membrane stabilization, attenuating the effects of oxidative stress. Objective: To evaluate the association between magnesium and concentrations of thiobarbituric acid reactive substances (TBARS) in patients with obesity and eutrophic women. Methods: A cross-sectional study was conducted with 73 women, divided into two groups: case group (patients with obesity, n=27) and control group (eutrophic women, n=46). Measurements of body mass index and waist circumference were performed. Dietary magnesium intake was assessed by the three-day food record using the NutWin software. Urinary magnesium concentration was measured by atomic absorption spectrophotometry method. Plasma concentrations of thiobarbituric acid reactive substances (TBARS) were also determined. Results: Mean values of dietary magnesium intake were 161.59 ± 60.04 and 158.73 ± 31.96 for patients with obesity and control group, respectively, with no significant difference between the groups studied (p >0.05). The value of urinary excretion of magnesium was lower than the reference values in both groups, with no significant difference between the groups studied (p >0.05). The plasma concentration of thiobarbituric acid reactive substances was significantly higher in patients with obesity compared to the control group (p <0.001). There was no correlation between levels of magnesium biomarkers and the concentration of TBARS (p >0.05). Conclusion: Patients with obesity showed a reduced dietary magnesium intake which seems to induce hypomagnesuria as a compensatory mechanism. The marker of oxidative stress evaluated in this study was not influenced by magnesium.

scholarly journals Clinical Significance of ROMs, OXY, SHp and HMGB-1 in Canine Leishmaniosis

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 754
Author(s):  
Michela Pugliese ◽  
Alessandra Sfacteria ◽  
Gaetano Oliva ◽  
Annastella Falcone ◽  
Manuela Gizzarelli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lymph Nodes ◽  
Clinical Signs ◽  
Biological Tissues ◽  
Stress Index ◽  
Control Group ◽  
Canine Leishmaniosis ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

This study aimed to investigate the role of oxidative stress parameters (ROMs, OXY, SHp), the Oxidative Stress index (OSi), and High Mobility Group Box-1 protein (HMGB-1) in canine leishmaniosis (CanL). For this study, thirty dogs, naturally infected with Leishmania spp. (Leishmania Group, LEISH) and ten healthy adult dogs (control group, CTR) were included. The diagnosis of CanL was performed by a cytological examination of lymph nodes, real time polymerase chain reaction on biological tissues (lymph nodes and whole blood), and an immunofluorescence antibody test (IFAT) for the detection of anti-Leishmania antibodies associated with clinical signs such as dermatitis, lymphadenopathy, onychogryphosis, weight loss, cachexia, lameness, conjunctivitis, epistaxis, and hepatosplenomegaly. The HMGB-1 and oxidative stress parameters of the LEISH Group were compared with the values recorded in the CTR group (Mann Whitney Test, p < 0.05). Spearman rank correlation was applied to evaluate the correlation between the HMGB-1, oxidative stress biomarkers, hematological and biochemical parameters in the LEISH Group. Results showed statistically significant higher values of SHp in the LEISH Group. Specific correlation between the ROMs and the number of red blood cells, and between HGMB-1 and SHp were recorded. These preliminary data may suggest the potential role of oxidative stress in the pathogenesis of CanL. Further studies are undoubtedly required to evaluate the direct correlation between inflammation parameters with the different stages of CanL. Similarly, further research should investigate the role of ROMs in the onset of anemia.

scholarly journals Therapeutic effects of simvastatin on Galectin-3 and oxidative stress parameters in endotoxemic lung tissue

2018 ◽  
Vol 38 (3) ◽  
Author(s):  
Hatice Yorulmaz ◽  
Elif Ozkok ◽  
Engin Kaptan ◽  
Gulten Ates ◽  
Sule Tamer
Keyword(s):  
Oxidative Stress ◽  
Lung Tissue ◽  
Thiobarbituric Acid ◽  
Statin Treatment ◽  
Therapeutic Effects ◽  
Acute Lung Inflammation ◽  
Oxidative Stress Parameters ◽  
Inflammatory Conditions ◽  
Galectin 3 ◽  
Stress Parameters

Galectins constitute of a soluble mammalian β-galactoside binding lectin family, which play homeostatic roles in the regulation of the cell cycle, and apoptosis, in addition to their inflammatory conditions. Galectin-3 has an important role in the regulation of various inflammatory conditions including endotoxemia, and airway inflammation. Statins, the key precursor inhibitors of 3-hydroxyl-3-methyl coenzyme A (HMG-CoA) reductase, may prevent the progression of inflammation in sepsis after prior statin treatment. Endotoxemia leads to the formation of oxidative stress parameters in proteins, carbohydrates, and DNA. In the present study, we aimed to show the effects of simvastatin on Galectin-3, and glutathione reductase (GR), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and thiobarbituric acid reactive substances (TBARS) levels in lung tissue of rats which were treated with lipopolysaccharides (LPS) during the early phase of sepsis. Rats were divided into four groups as the control, LPS (20 mg/kg), simvastatin (20 mg/kg), and simvastatin+LPS group. Galectin-3 expression in formalin-fixed paraffin-embedded lung tissue sections was demonstrated by using the immunohistochemistry methods. There were reduced densities, and the decreased number of Galectin-3 immunoreactivities in the simvastatin+LPS group compared with the LPS group in the pneumocytes, and in the bronchial epithelium of lung tissue. In the LPS group, GR, GSH-Px, and SOD were found lower than the levels in simvastatin-treated LPS group (P<0.05, P<0.01, P<0.01 respectively) in the lung tissue. However, TBARS decreased in the simvastatin+LPS group compared with the levels in LPS group (P<0.001). Simvastatin attenuates LPS-induced oxidative acute lung inflammation, oxidative stress, and suppresses LPS-induced Galectin-3 expression in the lung tissue.

scholarly journals Increased oxidative stress as a mechanism for decreased BDNF levels in acute manic episodes

2008 ◽  
Vol 30 (3) ◽  
pp. 243-245 ◽  
Author(s):  
Flávio Kapczinski ◽  
Benício N Frey ◽  
Ana C Andreazza ◽  
Márcia Kauer-Sant'Anna ◽  
Ângelo B M Cunha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bipolar Disorder ◽  
Neurotrophic Factor ◽  
Thiobarbituric Acid ◽  
Brain Derived Neurotrophic Factor ◽  
Oxidative Status ◽  
Control Group ◽  
Healthy Controls ◽  
Thiobarbituric Acid Reactive Substances ◽  
Low Levels

OBJECTIVE AND METHOD: There is a growing amount of data indicating that alterations in brain-derived neurotrophic factor and increased oxidative stress may play a role in the pathophysiology of bipolar disorder. In light of recent evidence demonstrating that brain-derived neurotrophic factor levels are decreased in situations of increased oxidative stress, we have examined the correlation between serum thiobarbituric acid reactive substances, a measure of lipid peroxidation, and serum brain-derived neurotrophic factor levels in bipolar disorder patients during acute mania and in healthy controls. RESULTS: Serum thiobarbituric acid reactive substances and brain-derived neurotrophic factor levels were negatively correlated in bipolar disorder patients (r = -0.56; p = 0.001), whereas no significant correlation was observed in the control group.. CONCLUSION: These results suggest that alterations in oxidative status may be mechanistically associated with abnormal low levels of brain-derived neurotrophic factor observed in individuals with bipolar disorder.

scholarly journals Protective effects of quercetin and vitamin C against nicotine-induced toxicity in the blood of Wistar rats

2016 ◽  
Vol 67 (4) ◽  
pp. 304-310 ◽  
Author(s):  
Milica G. Paunović ◽  
Branka I. Ognjanović ◽  
Miloš M. Matić ◽  
Andraš Š. Štajn ◽  
Zorica S. Saičić
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Liver Enzymes ◽  
Synergistic Effects ◽  
Lipid Peroxide ◽  
Saline Control ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

Abstract Nicotine is a potential inducer of oxidative stress, through which it can damage numerous biological molecules. The aim of our study was to investigate the prooxidative effects of nicotine and protective (additive or synergistic) effects of quercetin and vitamin C in the blood of experimental animals, to determine whether the combination of these antioxidants might be beneficial for clinical purposes. Wistar albino rats were receiving intraperitoneal nicotine injection (0.75 mg kg-1 per day) or saline (control group) or nicotine plus quercetin (40 mg kg-1 per day) and vitamin C (100 mg kg-1 per day) for three consecutive days. On day 4, we determined their blood lipid profile, liver enzymes, oxidative stress parameters, and antioxidative system parameters. Compared to untreated control, nicotine significantly increased total cholesterol, LDLcholesterol, triglycerides, liver enzymes (alanine transaminase, aspartate transaminase, and lactate dehydrogenase) and oxidative stress parameters (superoxide anion, hydrogen peroxide, and lipid peroxide) and decreased HDL-cholesterol, glutathione, and superoxide dismutase/catalase activity. Quercetin + vitamin C reversed these values significantly compared to the nicotine alone group. Our results confirm that nicotine has significant prooxidative effects that may disrupt the redox balance and show that the quercetin + vitamin C combination supports antioxidant defence mechanisms with strong haematoprotective activity against nicotine-induced toxicity. In practical terms, this means that a diet rich in vitamin C and quercetin could prevent nicotine-induced toxicity and could also be useful in the supportive care of people exposed to nicotine.

scholarly journals Effect of Intraperitoneal Etanercept on Oxidative Stress in Rats with Peritonitis

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Yasar Yildirim ◽  
Esma Gulsum Cellad ◽  
Ali Veysel Kara ◽  
Zülfükar Yilmaz ◽  
Ali Kemal Kadiroglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Control Group ◽  
Tissue Samples ◽  
Oxidative Stress Parameters ◽  
Group 4 ◽  
Peritoneal Tissue ◽  
Group 3 ◽  
Cefazolin Sodium ◽  
Group 2 ◽  
Stress Parameters

Our aim was to evaluate effect of etanercept on oxidative stress parameters in rats with experimental peritonitis and investigate the availability of etanercept usage in the treatment of peritonitis in the future. Twenty-eight rats were divided into four groups as control (group 1), peritonitis (group 2), peritonitis + cefazolin sodium (group 3), and peritonitis + cefazolin sodium + etanercept (group 4). Peritoneal tissue and blood samples were taken from all of the rats for histopathological and biochemical examination. The oxidative stress parameters were examined in blood and tissue samples. It was observed that rats with peritonitis benefit from cefazolin sodium treatment. Evaluating the effectiveness of etanercept was our main objective for this study. In this perspective, we compared group 3 and group 4 and found statistically significant decreases in oxidative parameters and statistically significant increases in antioxidants in serum and tissue samples in group 4. It is observed that there was a significant contribution of etanercept on biochemical and also histopathological results. As a result, the TNF-αinhibitor, etanercept, in addition to antibiotics given in the early treatment of peritonitis results in more significant improvement of histopathological and oxidative parameters as compared to antibiotics alone.

scholarly journals Hepatoprotective effect of desi and kabuli cultivars of Cicer arietinum L (chick peas) against carbon tetrachloride-induced toxicity in rats

2020 ◽  
Vol 19 (3) ◽  
pp. 609-615
Author(s):  
Mudasir Majeed ◽  
Abdullah Ijaz Hussain ◽  
Haseeb Anwar ◽  
Shahzad Irfan ◽  
Shahzad Ali Shahid Chatha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Tetrachloride ◽  
Cicer Arietinum ◽  
Control Group ◽  
Serum Samples ◽  
Cicer Arietinum L ◽  
Oxidative Stress Parameters ◽  
High Doses ◽  
Ethanol Extracts ◽  
Stress Parameters

Purpose: To determine the hepatoprotective potential of ethanol extracts of desi and kabuli cultivars of Cicer arietinum L. (chick peas). Methods: Hepatotoxicity was induced in rats using oral administration of carbon tetrachloride (CCl4). The rats were then orally administered different doses of the ethanol extracts of desi and kabuli cultivars of Cicer arietinum L. for 21 days. Oxidative stress parameters and hepatoprotective profiles were determined in serum samples using standard procedures. The effect of the treatments on liver histology was also determined. Results: Administration of extracts of desi and kabuli cultivars of Cicer arietinum L. to CCl4 treated rats at a dose of 300 mg/kg resulted in a significant (p ≤ 0.05) decrease in oxidative stress parameters, whereas catalase activity significantly increased (p ≤ 0.05); on the other hand, ALT and AST levels were decreased significantly (p ≤ 0.05), when compared to the control group. Conclusion: High doses of Cicer arietinum L (desi and kabuli cultivars) seem to have hepatoprotective and antioxidant effects on CCl4-induced toxicity in rats. This finding underscores the therapeutic importance of Cicer arietinum L. as a plant with hepatoprotective properties. Keywords: Cicer arietinum, Phenolics, Hepatotoxicity, Chick peas, Catalase

scholarly journals Oxidative stress induced by chlorpromazine in patients treated and acutely poisoned with the drug

2016 ◽  
Vol 73 (4) ◽  
pp. 312-317 ◽  
Author(s):  
Bratislav Dejanovic ◽  
Vesna Vukovic-Dejanovic ◽  
Ivana Stevanovic ◽  
Ivana Stojanovic ◽  
Gordana Mandic-Gajic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Serum Concentration ◽  
Control Group ◽  
Sod Activity ◽  
Antioxidative Status ◽  
Oxidative Stress Parameters ◽  
Long Time ◽  
Total Antioxidative Status ◽  
Therapeutic Doses ◽  
Stress Parameters

Background/Aim. Although chlorpromazine (CPZ) is an antipsychotic drug widely used in clinical practice for a long time, its mechanism of action has not been entirely defined. An extremely difficult managing of patients acutely poisoned with CPZ is additional reason for detailed studying its toxicity mechanisms. In this clinical study, we investigated whether the oxidative stress (OS) mediates CPZ toxic effects in the exposed patients. Methods. The patients were organized into 3 groups: the T-group - hospitalized patients receiving therapeutic doses of 75-150 mg CPZ/day; the overdosed group, divided into two subgroups: the group M and the group S - mildly (CPZ serum concentration: 0.21 ? 0.05 mg/L) and severely (CPZ serum concentration: 2.66 ? 0.25 mg/L) poisoned patients, respectively, and the group C (control group of healthy volunteers). Oxidative stress parameters [total antioxidative status (TAS) and malondialdehyde (MDA) in plasma)] and superoxide dismutase (SOD) activity in erythrocytes were measured spectrophotometrically, and CPZ concentrations in serum were monitored chromatographically. One set of measurements was performed in the group C and T, whereas two sets of measurements (after 24 hours and 48 hours) were done in the poisoned patients, groups M and S. Results. A decrease of TAS and increase of SOD activity were obtained in both subgroups of the poisoned patients, compared to the controls and the group receiving therapeutic doses of CPZ. A significant increase of MDA was achieved in severely poisoned patients, compared to all other groups. Conclusion. Changed oxidative stress parameters in patients poisoned with chlorpromazine indicate involvement of oxidative stress in the toxicity mechanism(s) of chlorpromazine.

The Effect of Borage (Echium amoenum) on the Mouse Heart and Hematology Parameters

2019 ◽  
Vol 19 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Parisa Sadighara ◽  
Atefeh Araghi ◽  
Behrouz Tajdar-oranj ◽  
Leila Peivasteh Roudsari ◽  
Afsaneh Mohajer ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Control Group ◽  
Mouse Heart ◽  
Potential Health ◽  
Oxidative Stress Parameters ◽  
Significant Difference ◽  
High Doses ◽  
Hematology Parameters ◽  
The Impact ◽  
Stress Parameters

Background: There has been considerable interest in the potential health benefits of borage. Little information is available regarding the safety of this plant. The purpose of this study was to evaluate the impact of borage on the mouse heart. Methods: Different amounts of borage extract were injected in mice. The mice were randomly divided into 4 groups including group1 (Control group without injection), group2, 3 and 4 that received 12.5 mg/kg, 25 mg/kg and 50 mg/kg respectively for 28 days. Oxidative stress parameters (lipid peroxidation, total glutathione groups assay and cupric assay) and biochemical (Creatine kinase activity and total cholesterol) and hematology parameters were evaluated. Furthermore, histopathology study was carried out on heart tissues. Results: We found that there was no significant difference in oxidative stress parameters and biochemical parameters between the control group and the groups that received different amounts of borage extract. There were also no changes in histopathology study. In blood parameters, the level of erythrocytes, hematocrit and hemoglobin decreased to 50mg/kg, whereas the level of MCH and MCV decreased in high doses. Conclusion: This article suggested that borage did not cause significant damage to the heart tissue in mice model. In hematology factors, significant changes were observed in erythrocytes and related parameters. Therefore, hematotoxicity of consumption this plant should be considered at high doses.

Effect of toluene on erythrocyte membrane stability under in vivo and in vitro conditions with assessment of oxidant/antioxidant status

2009 ◽  
Vol 25 (8) ◽  
pp. 545-550 ◽  
Author(s):  
Ismail Karabulut ◽  
Z. Dicle Balkanci ◽  
Bilge Pehlivanoglu ◽  
Aysen Erdem ◽  
Ersin Fadillioglu
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Unsaturated Fatty Acids ◽  
Osmotic Fragility ◽  
Human Erythrocytes ◽  
Antioxidant Enzyme Activities ◽  
Oxidative Stress Parameters ◽  
Stress Parameters

Toluene, an organic solvent used widely in the industry, is highly lipophilic and accumulates in the cell membrane impeding transport through it. Its metabolites cause oxygen radical formation that react with unsaturated fatty acids and proteins in erythrocytes leading to lipid peroxidation and protein breakdown. In this study, we aimed to investigate the membrane stabilizing and the oxidative stress—inducing effects of toluene in human erythrocytes. Measurements of osmotic fragility, mean corpuscular volume (MCV), oxidative stress parameters and antioxidant enzyme activities were performed simultaneously both in individuals exposed to toluene professionally (in vivo) and human erythrocytes treated with toluene (in vitro). To measure osmotic fragility, erythrocytes were placed in NaCl solutions at various concentrations (0.1% [blank], 0.38%, 0.40%, 0.42%, 0.44%, 0.46%, 0.48% and 1% [stock]). Percentage of haemolysis in each solution was calculated with respect to the 100% haemolysis in the blank solution. The erythrocyte packs prepared at the day of the above-mentioned measurements were kept at —80°C until the time for determination of malonyldialdehyde and protein carbonyl levels, and catalase (CAT) and glutathione peroxidase activities as indicators of oxidative stress. Toluene increased oxidative stress parameters significantly both in vivo and in vitro; it also caused a significant decrease in the activities of antioxidant enzymes. Osmotic fragility was altered only in the case of in vitro exposure. In conclusion, toluene exposure resulted in increased lipid peroxidation and protein damage both in vivo and in vitro. Although, it is natural to expect increased osmotic fragility due to oxidative properties of toluene, its membrane-stabilizing effect overcame the oxidative properties leading to decreased osmotic fragility or preventing its deterioration in vitro and in vivo toluene exposures, respectively, in the present study.

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