scholarly journals Chemical Composition and Biological Activity of Paris quadrifolia L.

2012 ◽  
Vol 67 (11-12) ◽  
pp. 565-570 ◽  
Author(s):  
Kristina Jenett-Siems ◽  
Nadin Krause ◽  
Karsten Siems ◽  
Sven Jakupovic ◽  
Gerd Wallukat ◽  
...  
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Structural Features ◽  
Model System ◽  
Cardiac Cells ◽  
Steroidal Saponin ◽  
Cellular Model ◽  
Potential Influence ◽  
Plant Compounds

A study of the components of Paris quadrifolia was undertaken to identify compounds with potential influence on cardiac cells, since previous reports suggested a cardiotoxic risk of this plant. Compounds isolated and identified included one new steroidal saponin, (23S,24S)- spirosta-5,25(27)-diene-1β,3β,21,23,24-pentol-1-O-β-D-apiofuranosyl-(1→3)-α-L-rhamnopyranosyl-( 1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranoside 21- O- β- D- apiofuranoside 24-O-β-D-fucopyranoside (1), demonstrating quite unusual structural features, as well as the known compounds 26-O-β-D-glucopyranosyl-(25R)-5-en-furost-3β,17α,22α,26-tetraol- 3- O- α- L- rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl - -(1→2)]- β- D- glucopyranoside (2), pennogenin 3-O-α-L-rhamnopyranosyl-(1→4)-α-L-rhamno pyranosyl- (1→4)-[α-L-rhamnopyranosyl-(1→ 2)]-β -D- glucopyranoside (3), 7- O- β- D-glucopyranosyl- kaempferol-3- O- β- D-glucopyranosyl-(1→2)-β- D-galactopyranoside (4), kaem pferol- 3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside (5), 5-hydroxyecdysterone (6), and 20-hydroxyecdysone (7). The pennogenin derivative 3 showed strong cardiotoxic effects in an in vitro cellular model system, whereas the respective furostanol derivative 2 was inactive.

scholarly journals STRUCTURE-ACTIVITY RELATIONSHIP: STUDY OF LEI-401 AS INHIBITOR OF NAPE-PLD BY PHARMACOPHORE MODEL

2021 ◽  
Vol 9 (5) ◽  
pp. 662-666
Author(s):  
Nilesh S. Kadu ◽  
Keyword(s):  
Metabolic Syndrome ◽  
Biological Activity ◽  
Phospholipase D ◽  
Pharmacophore Model ◽  
Structural Features ◽  
Pharmacophore Modeling ◽  
Physiological Processes ◽  
Structure Activity ◽  
Relationship Study

N-acyl-phosphatidylethanolamine phospholipase D (NAPE–PLD) is considered to be the principal enzyme that produces N-acylethanolamines (NAEs), a family of signaling lipids. NAEs are involved in numerous physiological processes such as appetite, satiety, pain, inflammation, fertility, stress, and anxiety. Furthermore, aberrant NAE levels are associated with metabolic syndrome and non-alcoholic steatohepatitis (NASH). Several inhibitors for NAPE–PLD have been reported. But most of the inhibitors showed poor to moderate potency for NAPE–PLD in vitro. Recently, Mario van der Stelt et al describe the SAR of NAPE–PLD inhibitors that afforded LEI–401 in vitro. However, no attempt was instigated to produce a consensus pharmacophore model of LEI–401 as inhibitors of NAPE–PLD. Pharmacophore modeling is an efficient and useful approach to identify important patterns in a series of molecules for optimizations. The consensus pharmacophore model revealed the importance of structural features and their correlation with the biological activity.

Immunological activities of highly purified isoforms of human FSH correlate with in vitro bioactivities

1993 ◽  
Vol 139 (3) ◽  
pp. 511-518 ◽  
Author(s):  
P. G. Burgon ◽  
D. M. Robertson ◽  
P. G. Stanton ◽  
M. T. W. Hearn
Keyword(s):  
Biological Activity ◽  
Amino Acid ◽  
Protein Content ◽  
Close Correlation ◽  
Structural Features ◽  
Total Protein Content ◽  
Wide Range ◽  
Specific Activities ◽  
Range Of Values

ABSTRACT In a recent study, a five- to eightfold range in human FSH radioreceptor activity (RRA) was documented for highly purified isoforms of FSH when the data were expressed on an FSH protein content basis as determined by amino acid analysis. This study examined the FSH in vitro bioactivity and immunoactivity of these preparations. FSH in vitro biological activity showed a five- to eightfold range in activity with a high correlation with the RRA values (r=0·82). A similar five- to eightfold range of values was obtained with a specific FSH radioimmunoassay and an FSH two-site immunoassay with high correlations again observed between each other, between each immunoassay and with either the in vitro bioassay or the RRA method (r=0·77–0·995). Although there was overall a close correlation between these assays, significant differences in ratios of activities between the in vitro bioassay and other methods were observed with highly purified FSH isoform preparations from different pI regions. The high correlation between in vitro bioassay/RRA methods and immunoassay methods over a wide range of isoform specific activities suggests that these methods are detecting similar structural features on each isoform. It is thus concluded that these immunoassays are not solely measuring hormone mass based entirely on amino acid composition. This conclusion raises questions about ratio measurements of FSH, where immunoassay methods are presumed to measure total protein content, and their application in physiological situations and clinical practice. Journal of Endocrinology (1993) 139, 511–518

Effect of cigarette filters on the chemical composition and in vitro biological activity of cigarette mainstream smoke

2009 ◽  
Vol 47 (1) ◽  
pp. 192-197 ◽  
Author(s):  
Han-Jae Shin ◽  
Hyung-Ok Sohn ◽  
Jung-Ho Han ◽  
Chul-Hoon Park ◽  
Hyeong-Seok Lee ◽  
...  
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Mainstream Smoke ◽  
Cigarette Mainstream Smoke

Differences in chemical composition predictive of in vitro biological activity among commercially important cultivars of genus Camellia

2019 ◽  
Vol 297 ◽  
pp. 124950
Author(s):  
Yijun Wang ◽  
Zhipeng Kan ◽  
Xiaochun Wan ◽  
John N. McGinley ◽  
Henry J. Thompson
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Commercially Important

scholarly journals Quality research of aqueous extracts of green and red tea

Chemija ◽  
2018 ◽  
Vol 29 (2) ◽  
Author(s):  
Kristina Ramanauskienė ◽  
Aidas Grigonis ◽  
Valdimaras Janulis ◽  
Raimondas Raudonis ◽  
Lina Babickaitė ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Biological Activity ◽  
Chemical Composition ◽  
Gallic Acid ◽  
Green Tea ◽  
Raw Material ◽  
Aqueous Extracts ◽  
Quality Research

Green and red tea due to the strong antioxidant, anti-inflammatory, anti-microbial, anti-tumor features are an intensive object of research. This article explores the chemical composition and biological activity of green and red tea of different fermentation. The preparation of aqueous extracts was implemented in household conditions, and the extraction time of release of active compounds from raw material was evaluated. The antibacterial activity was evaluated by the method in vitro. The research has shown that green tea is rich in catechins and caffeine. Red tea provides rutin and gallic acid. Green tea extracts inhibited the growth of S. aureus ir B. cereus. On the basis of the research results on the antioxidant activity, teas can be arranged in the following sequence: green > white > Oolong > red.

scholarly journals Biological activity of geopropolis produced by Partamona cupira (Meliponinae, Apidae) in the semiarid of the Brazilian northeast

2020 ◽  
Vol 9 (11) ◽  
pp. e1259119644
Author(s):  
Jael Soares Batista ◽  
Jardel Bezerra da Silva ◽  
Kizzy Millenn de Freitas Mendonça Costa ◽  
Tiago da Silva Teófilo ◽  
Natanael Silva Félix ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Chemical Composition ◽  
Assay Method ◽  
Semiarid Region ◽  
Control Group ◽  
High Biological Activity ◽  
High Antioxidant Activity ◽  
Brazilian Northeast

Research on the chemical composition and pharmacological activities of geopropolis produced by stingless bees (Hymenoptera, Apidae, Meliponini) may contribute to expand its use of propolis-based formulations in the clinical context. Thus he study aimed to evaluate the chemical composition and biological activity of the hydroethanolic extract (HEG) of the geopropolis of Partamona cupira, obtained in the semiarid region of northeast Brazil. Chemical analyses of HEG were carried out using HPLC-DADESI-MS/MS. The antioxidant activity of extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay method and cytotoxic activity by the in vitro MTT method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The antibacterial activity of the HEG was evaluated through the disc-diffusion test on agar and measurement of the promoted by the extract in different concentrations. The genoprotective potential of the HEG was evaluated through the comet assay on fibroblasts of L929, co-treated with the extract and submitted to genotoxicity induction with H2O2. We also investigated the healing effect of the cream containing geopropolis (10%) on experimental skin wounds in Wistar rats. The HEG presented in its composition phenolic compounds of high biological activity, as well as revealed high antioxidant activity and promoted genoprotective effect by reducing DNA damage from L929 fibroblasts. The HEG presented antimicrobial activity promoting inhibition of S. aureus, S. pyogenes, E. coli and E. aerogenes. The topical use of the cream containing geopropolis promoted wound closure and faster reepithelialization in relation to the control group, in addition to a less intense inflammatory reaction, increased fibroblastic proliferation and collagen deposition.

scholarly journals Chemical Composition and Biological Activity of Trapa Natans L.

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Rosa Cukic Corovic ◽  
Jovana Bradic ◽  
Marina Tomovic ◽  
Vera Dabanovic ◽  
Vladimir Jakovljevic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Chemical Composition ◽  
North America ◽  
Pharmacological Activity ◽  
In Vitro Antimicrobial Activity ◽  
Water Chestnut ◽  
Trapa Natans ◽  
Anti Inflammatory

Abstract Trapa natans L., water chestnut (indian name singada, montenegrin name kasaronja) belonging to Trapaceae family is annual aquatic floating herb native to Asia but widely distributed in the Europe and in North America. The presence of two species, Trapa natans L. and Trapa longicarpa M.Jank. ssp. scutarensis M.Jank has been confirmed on the Skadar lake in Montenegro. Trapa natans is a plant demonstrating promising pharmacological activity mostly due to the presence of its constituens, phenols and flavonoids, showed hepatoprotective potential, antioxidant, anti-inflammatory, antidiabetic, antifungal and in vitro antimicrobial activity.

scholarly journals Biological activity of some essential oil constituents in four Nepeta L. species against Sitophilus oryzae L.

2019 ◽  
Vol 20 (2) ◽  
pp. 338-343 ◽  
Author(s):  
SHAHLA AMINI ◽  
MAJID GHORBANI NOHOOJI ◽  
MOUSA KHANI ◽  
MOHAMMAD REZA LABBAFI ◽  
FARAHNAZ KHALIGHI-SIGAROODI
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Sitophilus Oryzae ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Nepeta Cataria ◽  
Natural Substances

Amini S, Nohooji MG, Khani M, Labbafi MR, Khalighi-Sigaroodi f. 2019. Biological activity of some essential oil constituents in four Nepeta L. species against Sitophilus oryzae L.. Biodiversitas 20: 338-343. Recently, there has been a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides. Essential oils are among the best-known natural substances with insecticidal properties. These compounds may act as fumigants, contact insecticides, repellents, antifeedants and may affect growth and development of the insects. In an attempt to find a natural and inexpensive method for the control of stored-product pests, contact toxicity of essential oils of Nepeta cataria, Nepeta pogonosperma, Nepeta glomerulosa. and Nepeta binaloudensis. were investigated on adult insects of Sitophilus oryzae L. (Coleoptera, Curculionidae) in vitro condition. Chemical Composition, repellency and fumigant toxicity of the essential oils were investigated. Chemical composition of the essential oils of above plants were identified by GC-MS. 4aα,7α,7aβ-nepetalactone (82.74 %) was major component of N. cataria and also different isomers of nepetalactone were among the major compounds in N. Pogonosperma and N. binaloudensis oils. In fumigants bioassay, N. glomerulosa (124.318μL/L air) showed the highest toxicity against S. oryzae adults, followed by N.pogonosperma (150.49μL/L air) and N. cataria (152.630μL/L air), respectively. Also, the S. oryzae was repelled by N. cataria (91.61%) and N. binaloudensis (91.50 %), respectively.

scholarly journals Composition and biological activity of triterpenes and steroids from Inonotus obliquus (chaga)

2016 ◽  
Vol 62 (4) ◽  
pp. 369-375 ◽  
Author(s):  
S.A. Nikitina ◽  
V.R. Khabibrakhmanova ◽  
M.A. Sysoeva
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Cell Lines ◽  
Natural Environment ◽  
Side Chain ◽  
Inonotus Obliquus ◽  
Common Features ◽  
Inhibition Of Growth

Data on the chemical composition of triterpenic and steroid compounds, isolated from the chaga mushroom grown in natural environment or in a synthetic culture have been summarized. Special attention has been paid to the biological activity of chaga mushroom extracts and these particular compounds against various cancer cell lines in vitro and in vivo. This analysis has demonstrated some common features in inhibition of growth of various cell lines by chaga mushroom components. In this context, the most active are triterpene compounds containing OH group at C-22 and a side chain unsaturated bond.

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