Quality research of aqueous extracts of green and red tea

Green and red tea due to the strong antioxidant, anti-inflammatory, anti-microbial, anti-tumor features are an intensive object of research. This article explores the chemical composition and biological activity of green and red tea of different fermentation. The preparation of aqueous extracts was implemented in household conditions, and the extraction time of release of active compounds from raw material was evaluated. The antibacterial activity was evaluated by the method in vitro. The research has shown that green tea is rich in catechins and caffeine. Red tea provides rutin and gallic acid. Green tea extracts inhibited the growth of S. aureus ir B. cereus. On the basis of the research results on the antioxidant activity, teas can be arranged in the following sequence: green > white > Oolong > red.

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Study on the protective effect of an innovative cow milk-based product against some human skin-bacterial pathogens

Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale ◽

10.4081/jbr.2018.7426 ◽

2018 ◽

Vol 91 (1) ◽

Author(s):

Carlo Cosentino ◽

Hazem Salaheldin Elshafie ◽

Cristiana Labella ◽

Carmine D'Adamo ◽

Giovanni Pecora ◽

...

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Biological Activity ◽

Chemical Composition ◽

Protective Effect ◽

Human Skin ◽

Bacterial Pathogens ◽

Cow Milk ◽

Inflammatory Factors ◽

Total Counting

There is a growing interest related to the possible use of some components of cow milk that behave as immunomodulators, exert biological activity, and have anti-inflammatory factors. The aims of the study were: i) to investigate the chemical composition and antioxidant activity of Podolian cow milk; ii) to test the efficacy of some hand soaps supplemented with different percentage of cow milk compared to placebo; iii) to evaluate the antibacterial activity of milk added to soap. Antioxidant activity of Podolian milk resulted in mean 97.03% and 52.09% utilizing 2,2’-azinobis 3-ethylbenzthiazoline-6-acid and 2,2-diphenyl-1-picrylhydrazyl methods, respectively. The efficacy test showed that the most effective soap was obtained adding 5% of Podolian milk. For this soap, total counting bacterial test evidenced the highest reduction of bacterial hand contamination assessed by 98%.

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Biological activity of geopropolis produced by Partamona cupira (Meliponinae, Apidae) in the semiarid of the Brazilian northeast

Research Society and Development ◽

10.33448/rsd-v9i11.9644 ◽

2020 ◽

Vol 9 (11) ◽

pp. e1259119644

Author(s):

Jael Soares Batista ◽

Jardel Bezerra da Silva ◽

Kizzy Millenn de Freitas Mendonça Costa ◽

Tiago da Silva Teófilo ◽

Natanael Silva Félix ◽

...

Keyword(s):

Antioxidant Activity ◽

Biological Activity ◽

Chemical Composition ◽

Assay Method ◽

Semiarid Region ◽

Control Group ◽

High Biological Activity ◽

High Antioxidant Activity ◽

Brazilian Northeast

Research on the chemical composition and pharmacological activities of geopropolis produced by stingless bees (Hymenoptera, Apidae, Meliponini) may contribute to expand its use of propolis-based formulations in the clinical context. Thus he study aimed to evaluate the chemical composition and biological activity of the hydroethanolic extract (HEG) of the geopropolis of Partamona cupira, obtained in the semiarid region of northeast Brazil. Chemical analyses of HEG were carried out using HPLC-DADESI-MS/MS. The antioxidant activity of extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay method and cytotoxic activity by the in vitro MTT method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The antibacterial activity of the HEG was evaluated through the disc-diffusion test on agar and measurement of the promoted by the extract in different concentrations. The genoprotective potential of the HEG was evaluated through the comet assay on fibroblasts of L929, co-treated with the extract and submitted to genotoxicity induction with H2O2. We also investigated the healing effect of the cream containing geopropolis (10%) on experimental skin wounds in Wistar rats. The HEG presented in its composition phenolic compounds of high biological activity, as well as revealed high antioxidant activity and promoted genoprotective effect by reducing DNA damage from L929 fibroblasts. The HEG presented antimicrobial activity promoting inhibition of S. aureus, S. pyogenes, E. coli and E. aerogenes. The topical use of the cream containing geopropolis promoted wound closure and faster reepithelialization in relation to the control group, in addition to a less intense inflammatory reaction, increased fibroblastic proliferation and collagen deposition.

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Chemical composition, antioxidant activity and in vitro antibacterial activity of Achillea wilhelmsii C. Koch essential oil on methicillin-susceptible and methicillin-resistant Staphylococcus aureus spp.

3 Biotech ◽

10.1007/s13205-014-0197-x ◽

2014 ◽

Vol 5 (1) ◽

pp. 39-44 ◽

Cited By ~ 16

Author(s):

Seyedeh Mahsan Hoseini Alfatemi ◽

Javad Sharifi Rad ◽

Majid Sharifi Rad ◽

Sasan Mohsenzadeh ◽

Jaime A. Teixeira da Silva

Keyword(s):

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Methicillin Resistant Staphylococcus Aureus ◽

Methicillin Resistant ◽

In Vitro Antibacterial Activity ◽

Achillea Wilhelmsii

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In vitro activities of Acacia nilotica (L.) Delile bark fractions against Oral Bacteria, Glucosyltransferase and as antioxidant

BMC Complementary Medicine and Therapies ◽

10.1186/s12906-020-03147-4 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Ali Mahmoud Muddathir ◽

Ebtihal Abdalla M. Mohieldin ◽

Tohru Mitsunaga

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Gallic Acid ◽

Crude Extract ◽

Inhibitory Activity ◽

Oral Bacteria ◽

Acacia Nilotica ◽

Assay Method ◽

Medicinal Uses ◽

Wide Range

Abstract Background Dental caries and periodontal disease are the most common chronic infectious oral diseases in the world. Acacia nilotica was commonly known in Sudan as Garad or Sunt has a wide range of medicinal uses. In the present study, antibacterial activity of oral bacteria (Streptococcus sobrinus and Porphyromonas gingivalis), inhibitory activity against glucosyltransferase (GTF) enzyme and antioxidant activity were assayed for methanolic crude extract of A. nilotica bark and its fractions. Methods Methanoilc crude extract of A. nilotica bark was applied to a Sephadex LH-20 column and eluted with methanol, aqueous methanol, and finally aqueous acetone to obtain four fractions (Fr1- Fr4). Furthermore, the crude extract and fractions were subjected to analytical high performance liquid chromatography (HPLC). The crude extract and its fractions were assayed for antibacterial activity against S. sobrinus and P. gingivalis using a microplate dilution assay method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), as well as GTF inhibition and antioxidant activity using ABTS radical scavenging method. Results Fractions (Fr1 and Fr2) exhibited MIC values of 0.3 mg/ml against the P. gingivalis. Additionally, Fr2 displayed MBC value of 1 mg/ml against two types of bacteria. Fr4 showed an especially potent GTF inhibitory activity with IC50 value of 3.9 μg/ml. Fr1 displayed the best antioxidant activity with IC50 value of 1.8 μg/ml. The main compound in Fr1 was identified as gallic acid, and Fr2 was mostly a mixture of gallic acid and methyl gallate. Conclusions The results obtained in this study provide some scientific rationale and justify the use of this plant for the treatment of dental diseases in traditional medicine. A. nilotica bark, besides their antibacterial potentiality and GTF inhibitory activity, it may be used as adjuvant antioxidants in mouthwashes. Further studies in the future are required to identify the rest of the active compounds.

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Chemical Composition, In Vitro and In Silico Antioxidant Potential of Melissa officinalis subsp. officinalis Essential Oil

Antioxidants ◽

10.3390/antiox10071081 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1081

Author(s):

Matilda Rădulescu ◽

Călin Jianu ◽

Alexandra Teodora Lukinich-Gruia ◽

Marius Mioc ◽

Alexandra Mioc ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

In Silico ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Inhibitory Effect ◽

Melissa Officinalis ◽

Β Carotene

The investigation aimed to study the in vitro and in silico antioxidant properties of Melissa officinalis subsp. officinalis essential oil (MOEO). The chemical composition of MOEO was determined using GC–MS analysis. Among 36 compounds identified in MOEO, the main were beta-cubebene (27.66%), beta-caryophyllene (27.41%), alpha-cadinene (4.72%), caryophyllene oxide (4.09%), and alpha-cadinol (4.07%), respectively. In vitro antioxidant properties of MOEO have been studied in 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging, and inhibition of β-carotene bleaching assays. The half-maximal inhibitory concentration (IC50) for the radical scavenging abilities of ABTS and DPPH were 1.225 ± 0.011 μg/mL and 14.015 ± 0.027 μg/mL, respectively, demonstrating good antioxidant activity. Moreover, MOEO exhibited a strong inhibitory effect (94.031 ± 0.082%) in the β-carotene bleaching assay by neutralizing hydroperoxides, responsible for the oxidation of highly unsaturated β-carotene. Furthermore, molecular docking showed that the MOEO components could exert an in vitro antioxidant activity through xanthine oxidoreductase inhibition. The most active structures are minor MOEO components (approximately 6%), among which the highest affinity for the target protein belongs to carvacrol.

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Chemical Composition and Antibacterial and Antioxidant Activity of a Citrus Essential Oil and Its Fractions

Molecules ◽

10.3390/molecules26102888 ◽

2021 ◽

Vol 26 (10) ◽

pp. 2888

Author(s):

Carmen M. S. Ambrosio ◽

Gloria L. Diaz-Arenas ◽

Leidy P. A. Agudelo ◽

Elena Stashenko ◽

Carmen J. Contreras-Castillo ◽

...

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Biological Activities ◽

E Coli ◽

Beneficial Bacterium ◽

Minor Compounds ◽

Antibacterial And Antioxidant Activity ◽

High Relative Amount

Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacterial activity results showed F4 was the only fraction exhibiting this activity, which was selective and higher activity on a pathogenic bacterium (E. coli) than on a beneficial bacterium (Lactobacillus sp.). However, F4 activity was lower than BOT. Similarly, F4 displayed the highest antioxidant activity among fractions (equivalent to BOT). These results indicated that probably those minor compounds that detected in F4 would be more involved in conferring the biological activities for this fraction and consequently for the whole BOT, instead of the major compound, limonene, playing this role exclusively.

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Impact of Harvest Conditions and Host Tree Species on Chemical Composition and Antioxidant Activity of Extracts from Viscum album L.

Molecules ◽

10.3390/molecules26123741 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3741

Author(s):

Wioleta Pietrzak ◽

Renata Nowak

Keyword(s):

Antioxidant Activity ◽

Biological Activity ◽

Chemical Composition ◽

Tree Species ◽

Plant Material ◽

Plant Secondary Metabolites ◽

Host Tree ◽

Host Tree Species ◽

High Antioxidant Activity ◽

Mistletoe Extracts

The content of plant secondary metabolites is not stable, and factors such as the region/location effect and seasonal variations have an impact on their chemical composition, especially in parasitic plants. Research in this area is an important step in the development of quality parameter standards of medicinal plants and their finished products. The effects of the time and place of harvest and the host tree species on the chemical composition and antioxidant activity of mistletoe extracts were investigated. Statistical tools were used to evaluate the results of the spectrophotometric and LC-ESI-MS/MS studies of the phenolic composition and antioxidant activity. The investigations indicate that the qualitative and quantitative composition, influencing the biological activity of mistletoe extracts, largely depends on the origin of the plant. The mistletoe extracts exhibited a rich phenol profile and high antioxidant activity. The chemometric analysis indicated that mistletoe collected from conifers (Viscum abietis and Viscum austriacum) had the most advantageous chemical composition and antioxidant activity. Moreover, the chemical profile and biological activity of the plant material were closely related to the climatic conditions and location of the harvested plant. Higher levels of phenolic compounds and high antioxidant activity were found in extracts obtained from plant material collected in cold weather with the presence of snow and less sunshine (autumn–winter period).

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In vitro susceptibility of the Sporothrix brasiliensis to aqueous extracts of the green tea (Camellia sinensis L. Kuntze)

Acta Veterinaria Brasilica ◽

10.21708/avb.2015.9.4.5414 ◽

2016 ◽

Vol 9 (4) ◽

Keyword(s):

Green Tea ◽

Camellia Sinensis ◽

Aqueous Extracts ◽

In Vitro Susceptibility ◽

Sporothrix Brasiliensis

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ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF STEM BARK ESSENTIAL OIL CONSTITUENTS OF LITSEA GLUTINOSA C. B. ROB.

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.13577 ◽

2016 ◽

Vol 8 (12) ◽

pp. 258

Author(s):

Arunodaya H. S. ◽

Krishna V. ◽

Shashikumar R. ◽

Girish Kumar K.

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Stem Bark ◽

Diffusion Method ◽

Vibrio Cholera ◽

Litsea Glutinosa ◽

Β Carotene ◽

Mic Value

Objective: To evaluate the chemical composition, antibacterial and antioxidant properties of stem bark essential oil of Litsea glutinosa C. B. Rob.Methods: The essential oil isolated from stem bark of L. glutinosa and their chemical composition was analyzed by gas chromatography coupled with mass spectrometry detector. The in vitro antibacterial activity of the stem bark essential oil was investigated against eight human pathogenic bacterial clinical isolates using agar disc diffusion method and MIC value was determined by modified resazurin microtitre-plate assay. The antioxidant activity of essential oil was measured by 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 2-azinobis-3-ethylbenzothiazoline-6-sulphonate radical cation (ABTS) and β-carotene bleaching assay.Results: GC-MS analysis of stem bark essential oil resulted in the identification of 37 compounds, off which 9,12-octadecadienoic acid (62.57%), hexadecanoic acid (12.68%), stigmast-5-en-3-ol (6.87%) and vitamin E (2.51%) were the main constituents representing 84.63% of the oil. The determination of in vitro antibacterial activity of stem bark essential oil resulted in significant inhibition zone (15.00±0.57 mm) and MIC value (0.15±0.15×10-2 mg/ml) against the pathogenic bacteria Vibrio cholera followed by Pseudomonas aeruginosa and Salmonella typhi. The results of DPPH radical scavenging (IC50:4.540±0.06 µg/ml), ABTS (IC50:256.02±0.06 µg/ml) and β-carotene bleaching assay (%I: 78.51±0.42 %) showed significant in vitro antioxidant property.Conclusion: L. glutinosa stem bark essential oil showed potential antibacterial activity against the Vibrio cholera. The results of this investigation supported the ethnomedical claim of essential oil as a demulcent, antidiarrheal and antioxidant drug.

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Aktivitas Antibakteri dan Antioksidan Asam Galat dari Kulit Buah Lokal yang Diproduksi dengan Tanase

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.14.1.11300.148-159 ◽

2018 ◽

Vol 14 (1) ◽

pp. 148

Author(s):

Eka Junaidi ◽

Yunita Arian Sani Anwar

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Antibacterial Activity ◽

Gallic Acid ◽

Diffusion Method ◽

Syzygium Cumini ◽

Garcinia Mangostana ◽

Dpph Method ◽

Fruit Waste

Penelitian ini merupakan lanjutan dari penelitian sebelumnya tentang produksi asam galat dari limbah kulit buah lokal di Lombok. Limbah kulit buah lokal yang digunakan yaitu kulit buah kepundung (Baccaurea racemosa Muell.Arg), kulit buah juwet (Syzygium cumini), dan kulit buah manggis (Garcinia mangostana). Pada penelitian ini dilakukan pengujian asam galat yang dihasilkan, untuk mengetahui aktivitas antioksidan dan antibakteri. Pengujian sifat antioksidan dilakukan dengan menggunakan metode DPPH, sedangkan aktivitas antibakteri diuji dengan menggunakan metode difusi agar pada bakteri Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa asam galat dari ketiga kulit buah memiliki aktivitas antioksidan. Asam galat dari kulit buah kepundung, kulit buah juwet dan kulit buah manggis memiliki IC50 masing-masing sebesar 5.95; 5,96; dan 5,92 ppm. Berbeda dengan kemampuan sebagai antioksidan, asam galat resistan terhadap bakteri Escherichia coli dan Staphylococcus aureus yang menunjukkan bahwa asam galat tidak memiliki aktivitas sebagai antibakteri.Antibacterial and antioxidant activity of gallic acid from peel local fruit which produced by tannase. This research is a continuation of the previous research about the gallic acid production from peel fruit local in Lombok. The local fruit waste used are kepundung rind (Baccaurea racemosa Muell.Arg), juwet rind (Syzygium cumini) and mangosteen rind (Garcinia mangostana). In this research, the produced Gallic acid was tested to determine its antioxidant and antibacterial activity. The antioxidant test conducted by DPPH method, meanwhile, the antibacterial activity was performed by agar diffusion method to bacteria Escherichia coli and Staphylococcus aureus. The results show that the produce gallic acid from those three rind provide antioxidant activity. Gallic acid from kepundung rind, juwet rind, and mangosteen rind have IC50 values of 5,95; 5,96; and 5,92, respectively. Gallic acid not showed inhibitory actions against the growth of Escherichia coli and Staphylococcus aureus. This result show that gallic acid from local fruit waste did not provide an antibacterial activity.

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