Potential of the electrical conductivity of seed soak water and early counts of radicle emergence to assess seed quality in some native species

Seed quality may be affected by several factors, including permeability, color, and lignin content in the seed coat. This study aimed at evaluating influence of lignin content in the tegument of seed samples of six different soybean cultivars, in which half of each sample was inoculated with the fungus Aspergillus flavus, on the physical and physiological quality, and on the seed health, during 180 days storage period, under cold chamber with controlled conditions of temperature and RH. For that, at each interval of 60 days, samples were removed, and the physiological quality of these seeds was assessed by means of moisture and lignin contents; and by tests of seed health, germination, and electrical conductivity. The moisture content of seeds remained constant during all storage period. In the seed health test, it was found that inoculation was efficient, once the minimum incidence of the fungus in the inoculated seeds was 85%. In the germination test, there was a trend of reduction on percentage germination with the increase in storage period. However, there was an increase on electrical conductivity of seeds assessed. It was concluded that there is no interference of the lignin content in the seed coat on the resistance to infection by the fungus Aspergillus flavus, even after seed storage for a period of 180 days.

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Electrical conductivity test in the evaluation of the physiological potential of treated and stored soybean seeds

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n6p3135 ◽

2021 ◽

Vol 42 (6) ◽

pp. 3135-3148

Author(s):

Ana Paula Silva Couto ◽

◽

Cristian Rafael Brzezinski ◽

Julia Abati ◽

Ronan Carlos Colombo ◽

...

Keyword(s):

Electrical Conductivity ◽

Seed Treatment ◽

Seed Quality ◽

Soybean Seed ◽

Accelerated Aging ◽

Control Treatment ◽

Soybean Seeds ◽

Seed Treatments ◽

Chemical Treatments ◽

Soybean Cultivars

Soybean seed treatment contributes to the maintenance of seed quality, but the effect of commercial formulations and chemical products on the effectiveness of the electrical conductivity test based on electrolyte leaching has been frequently questioned. This study aimed to verify the interference of the chemical seed treatment of two soybean cultivars on the effectiveness of the electrical conductivity test in evaluating the vigor of freshly treated and stored seeds. The experimental design was completely randomized, consisting of seven seed treatments and two evaluation periods (0 and 60 days after storage), with four replications. The used seed treatments consisted of 1) fipronil + pyraclostrobin + thiophanate-methyl, 2) imidacloprid + thiodicarb + carbendazim + thiram, 3) abamectin + thiamethoxan + fludioxonil + mefenoxam + thiabendazole, 4) carbendazim + thiram, 5) fludioxonil + mefenoxam + thiabendazole, 6) carboxin + thiram, and 7) control (no treatment). The cultivars were BRS 360 RR and BRS 284, which were analyzed separately. Germination, accelerated aging, emergence, and electrical conductivity tests were carried out. No differences were detected between the control and chemical treatments performed on seeds of the two freshly treated soybean cultivars regarding germination, accelerated aging, and emergence tests. The germination test stood out after storage with the cultivar BRS 360 RR, showing the maintenance of germination potential for seeds treated with carbendazim + thiram and the control treatment. Therefore, the chemical treatment of soybean seeds interferes with the result of the electrical conductivity test. The electrical conductivity test is effective in segregating seed lots in terms of vigor level. The electrical conductivity test correlates with the other vigor tests used to identify the reduction in the physiological seed quality with storage.

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Effect of Seed Treatments and Containers on Chilli and Brinjal Seed Viability

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2019/v36i230221 ◽

2019 ◽

pp. 1-9

Author(s):

Sunil Kumar ◽

Suryapal Singh ◽

S. S. Jakhar

Keyword(s):

Electrical Conductivity ◽

Seed Treatment ◽

Seed Quality ◽

Seed Viability ◽

Quality Parameters ◽

Packaging Material ◽

Packaging Materials ◽

Ambient Conditions ◽

Pathology Laboratory ◽

Seed Pathology

An experiment was conducted to investigate the influence of fungicides and packaging materials on longevity of chilli (variety: RCH-1) and brinjal (variety: Hisar Shyamal) seeds. The seeds were treated with 15 fungicides and were kept in three containers viz. Metal box, Cloth bag and Plastic zipling bag up to 12 months under ambient conditions in seed pathology laboratory of Department of Seed Science & Technology, CCSHAU, Hisar, India. The samples were drawn at quarterly intervals for ascertaining the seed quality parameters. The seeds treated with flusilazole and carbendazim (2 g kg-1 seed) in case of chilli and brinjal and stored in metal-box were found better for maintenance of higher seed quality parameters [electrical conductivity and dehydrogenase activity] during the study period. The study suggested that use of appropriate packaging material and seed treatment could be useful to prolong the storage life of chilli and brinjal seeds.

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Physiological quality of mechanically harvested chickpea seeds

Revista Engenharia na Agricultura - REVENG ◽

10.13083/reveng.v29i1.10907 ◽

2021 ◽

Vol 29 ◽

pp. 28-35

Author(s):

Letícia Betânia Xavier Dias ◽

Pedro Afonso de Melo Queiroz ◽

Thaís Cardoso de Castro ◽

Marco Antonio Moreira de Freitas ◽

Érica Fernandes Leão-Araújo ◽

...

Keyword(s):

Electrical Conductivity ◽

Significant Interaction ◽

Seed Quality ◽

Rotation Speed ◽

Mechanical Damage ◽

Seed Vigor ◽

Rotor Rotation ◽

Germination Speed ◽

Physiological Quality

Our objective was to evaluate the occurrence of mechanical damage in chickpea seeds with the combination of two harvester speeds (2.5 and 3.5 km.h-1) and three adjustments for rotor rotation speeds (500, 700, and 850 rpm). Harvesting was carried out in a seed production field. Seeds were evaluated for purity, germination (G), first count (FC), germination speed index (GSI), hypochlorite, electrical conductivity, and tetrazolium tests. There was an effect of harvester speeds on seed physiological quality for the first count (FC), germination (G), germination speed index (GSI), especially when combining with high rotor rotation speed. In these cases, the speed of 2.5 km.h-1 resulted in lower values. This harvester speed also had worse results when combining with 850 rpm for purity and hypochlorite tests. The tetrazolium test was not efficient in identifying differences in seed quality. There was no significant interaction between harvester speeds and rotor rotation speeds for the conductivity test. Evaluating the harvester speed’s isolated effect (3.5 km.h-1), we identified problems in seed vigor due to the higher value of exudates in the electrical conductivity test. Low harvester speed (2.5 km.h-1) associated with high rotor rotation speeds (700 and 850 rpm) causes a reduction of the physical and physiological quality of seeds.

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The Loss of Vigour and Sowing Value of Yellow Lupin Seeds (Lupinus luteus L.) as a Result of Mechanical Harvesting

Plant Breeding and Seed Science ◽

10.1515/plass-2016-0005 ◽

2016 ◽

Vol 73 (1) ◽

pp. 53-62

Author(s):

Agnieszka Faligowska ◽

Grażyna Szymańska ◽

Katarzyna Panasiewicz

Keyword(s):

Electrical Conductivity ◽

Seed Quality ◽

Pearson Correlation ◽

Mean Value ◽

Lupinus Luteus ◽

Pearson Correlation Coefficient ◽

Yellow Lupin ◽

Lupin Seeds ◽

The Mean

AbstractThe aim of the study was to determine the effect of mechanical harvest on the seed quality of yellow lupin. Two effects were studied: the cultivar of yellow lupin (the indeterminate cultivar - Mister and the determinate cultivar - Perkoz) and harvest methods: hand-picked plants with manual shelling of seeds as a control and mechanical shelling with a plot harvester. In comparison with manual shelling of seeds, the mechanical harvest reduced the seed germination and increased the number of abnormal seeds both cultivars. Determinate cultivar was more sensitive, because the loss of its quality was higher (germination of 10%) than indeterminate cultivar (6%). Perkoz had also higher electrical conductivity, with the mean value of 34.3 μS × cm−1× g−1. Manual shelled seeds were characterized by a significantly lower leakage of exudates (24.9 μS × cm−1× g−1), and hence, it exhibited greater vigour than mechanical harvested seeds. Most relations of Pearson correlation coefficient between vigour tests and germination were strong or practically functional.

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Electrical Conductivity as a Measure of Planting Seed Quality in Cotton 1

Agronomy Journal ◽

10.2134/agronj1987.00021962007900010030x ◽

1987 ◽

Vol 79 (1) ◽

pp. 147-152 ◽

Cited By ~ 2

Author(s):

N. W. Hopper ◽

H. R. Hinton

Keyword(s):

Electrical Conductivity ◽

Seed Quality

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Conditioning in the promotion and uniformization of Umbu seed germination

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452018109 ◽

2018 ◽

Vol 40 (1) ◽

Author(s):

Renato Teo Barros ◽

Cibele Chalita Martins ◽

Francisco Elder Carlos Bezerra Pereira ◽

Givanildo Zildo da Silva

Keyword(s):

Electrical Conductivity ◽

Seed Germination ◽

Water Content ◽

Environmental Conditions ◽

Seed Quality ◽

Germination Rate ◽

Ambient Conditions ◽

Germination Test ◽

Mean Germination Time ◽

Cold Chamber

Abstract Seed dormancy may decrease during storage and some environmental conditions may accelerate this process. The aim of this work was to determine efficient techniques to condition umbu seeds in order to promote and standardize their germination. Seeds were stored for 180 days in paper bags kept in five ambient conditions: laboratory (25 ºC and 55% RH); warm oven (40 °C and 53% RH); hot oven (50 °C and 49% RH); dry chamber (18 °C and 65% RH) and cold chamber (10 °C and 65% RH). Seed quality was evaluated every 60 days by means of the following tests and determinations: water content; germination test (25 °C and 55% RH, weekly evaluated up to 91 days after sowing); first count test (14 days); germination rate index; mean germination time and electrical conductivity. The conditioning of umbu seeds in laboratory, or in warm oven (40 ºC) used efficient techniques to promote and standardize germination; under these conditions, after six months of storage, germination increased from 31% to 84 and 74%, respectively.

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Seed Quality of Stored Soybean (Glycine max L.) as Influenced by Storage Containers and Storage Periods

The Agriculturists ◽

10.3329/agric.v12i1.19585 ◽

2014 ◽

Vol 12 (1) ◽

pp. 85-95 ◽

Cited By ~ 1

Author(s):

N Akter ◽

MM Haque ◽

MR Islam ◽

KM Alam

Keyword(s):

Electrical Conductivity ◽

Moisture Content ◽

Seedling Growth ◽

Seed Weight ◽

Seed Quality ◽

Dry Weight ◽

Germination Index ◽

Storage Container ◽

Storage Containers

An experiment was conducted at Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during April 2013 to June 2013 to study the effect of three storage containers (tin container, polythene bag and cloth bag); and five storage periods (0, 15, 30, 45, and 60 days) on the seed quality of soybean. The experiment was laid out in Completely Randomized Design (CRD) with four replications. The soybean seeds stored in tin container showed maximum germination capacity with high germination index, highest seedling growth, seedling dry weight per plant and vigour index. The seeds stored in cloth bag had the lowest seed quality during the testing period. The highest 1000-seed weight, moisture content, electrical conductivity, abnormal seedlings and number of dead seeds were recorded in cloth bag; where the equivalent lowest values were recorded in tin container. The moisture content, 1000-seed weight, electrical conductivity, number of abnormal seedlings and dead seeds were increased with the longer period of storage. In addition, germination index, seedling growth, dry weight per plant of soybean seedling, and vigor index decreased with the increase in storage period. Among the three containers, tin container was the best and the cloth bag was the worst storage container for soybean seed storage for long period. DOI: http://dx.doi.org/10.3329/agric.v12i1.19585 The Agriculturists 2014; 12(1) 85-95

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Seed Vigour Tests to Estimate Seedling Emergence in Cress (Lepidium sativum L.) Seed Lots

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47311453 ◽

2019 ◽

Vol 47 (3) ◽

Author(s):

Ibrahim DEMIR ◽

Burcu B. KENANOGLU ◽

Eren ÖZDEN

Keyword(s):

Electrical Conductivity ◽

Seedling Emergence ◽

Accelerated Aging ◽

Lepidium Sativum ◽

Accelerated Ageing ◽

Digital Object Identifier ◽

Seed Vigour ◽

Significance Levels ◽

Radicle Emergence ◽

Lepidium Sativum L

This work was carried out to estimate field and controlled room seedling emergence potential through seed vigour tests in cress (Lepidium sativum L.) seeds. Early radicle emergence percentages after 12 (RE12h), 24 (RE24h) and 36 (RE36h) hours of germination test, mean germination time, accelerated aging (45 °C, 100% RH, 24 h), electrical conductivity (EC) of soaking water (40 ml, 50 seeds, 20 °C), after 16 hours (EC16h) and 24 hours (EC24h), and EC16h and EC24h after accelerated ageing (AA, 45 °C, 100% RH, 24h) were tested as vigour tests in ten commercial seed lots of cress. Standard laboratory germination ranged between 88 and 93%. Seeds were sown on two occasions in field and controlled room conditions, and seedling emergence percentages were determined after 30 days in the soil and 14 days in the controlled room. Seedling emergence ranged between 67 and 85% and 59 and 83% in the first and second sowings in the field. These values were 75 and 92% in controlled room sowing. Vigour tests were correlated to seedling emergence potential at various significance levels but RE24h and EC16h showed the highest correlation coefficient values (p < 0.001) in all three sowing conditions as r = 0.879-0.988 in RE24h, and r = 0.902-0.962 in EC16h. Results indicated that early radicle emergence percentages after 24 hours (RE24h) and electrical conductivity value after 16 hours (EC16h) can be successfully used to estimate the seedling emergence potential of cress seeds in field and controlled room conditions. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Different methods for beech seed quality testing

Sustainable Forestry: Collection ◽

10.5937/sustfor1877001k ◽

2018 ◽

pp. 1-10

Author(s):

Ivona Kerkez ◽

Slavko Pavlović ◽

Aleksandar Lučić ◽

Jovana Devetaković ◽

Mirjana Šijačić-Nikolić ◽

...

Keyword(s):

Electrical Conductivity ◽

Hydrogen Peroxide ◽

Seed Weight ◽

Seed Quality ◽

Pearson Correlation ◽

Hydrogen Peroxide Treatment ◽

Quality Testing ◽

Nursery Production ◽

Comprehensive Determination ◽

Tetrazolium Test

This paper presents three different methods for beech seed quality testing. The aim of this study is to determine the correlation between the electrical conductivity test, tetrazolium test and hydrogen peroxide treatment. The seed was collected in 12 different seed stands in Serbia that are commonly used for nursery production. The seed sample used for testing was randomly taken from the total amount of the collected seed. The analysis of the obtained results included the calculation of the Pearson correlation coefficient in the statistical software package "Statistica 7". The correlation was determined between the recorded results of the seed weight and the electrical conductivity; the seed weight and the tetrazolium test; the seed weight and the hydrogen peroxide treatment; the electrical conductivity and the tetrazolium test; the electrical conductivity and the hydrogen peroxide treatment; the tetrazolium test and the hydrogen peroxide treatment. Based on the conducted researches it can be concluded that the standardization of the electrical conductivity test using the tetrazolium test and hydrogen peroxide treatment could contribute to a more quality and more comprehensive determination of the beech seed quality for commercial purposes.

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