scholarly journals Impacts of obesity, maternal obesity and nicotinamide mononucleotide supplementation on sperm quality in mice

Reproduction ◽  
2019 ◽  
Vol 158 (2) ◽  
pp. 171-181 ◽  
Author(s):  
Neil A Youngson ◽  
G Mezbah Uddin ◽  
Abhirup Das ◽  
Carl Martinez ◽  
Haley S Connaughton ◽  
...  
Keyword(s):  
Body Weight ◽  
Maternal Obesity ◽  
Sperm Quality ◽  
Sperm Count ◽  
Control Diet ◽  
Male Offspring ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Female Mice ◽  
Nicotinamide Mononucleotide

Male fertility and sperm quality are negatively impacted by obesity. Furthermore, recent evidence has shown that male offspring from obese rat mothers also have reduced sperm quality and fertility. Here, we extend work in this area by comparing the effects of both maternal obesity and offspring post-weaning diet-induced obesity, as well as their combination, on sperm quality in mice. We additionally tested whether administration of the NAD+-booster nicotinamide mononucleotide (NMN) can ameliorate the negative effects of obesity and maternal obesity on sperm quality. We previously showed that intraperitoneal (i.p.) injection of NMN can reduce the metabolic deficits induced by maternal obesity or post-weaning dietary obesity in mice. In this study, female mice were fed a high-fat diet (HFD) for 6 weeks until they were 18% heavier than a control diet group. Thereafter, HFD and control female mice were mated with control diet males, and male offspring were weaned into groups receiving control or HFD. At 30 weeks of age, mice received 500 mg/kg body weight NMN or vehicle PBS i.p. for 21 days. As expected, adiposity was increased by both maternal and post-weaning HFD but reduced by NMN supplementation. Post-weaning HFD reduced sperm count and motility, while maternal HFD increased offspring sperm DNA fragmentation and levels of aberrant sperm chromatin. There was no evidence that the combination of post-weaning and maternal HFD exacerbated the impacts in sperm quality suggesting that they impact spermatogenesis through different mechanisms. Surprisingly NMN reduced sperm count, vitality and increased sperm oxidative DNA damage, which was associated with increased NAD+ in testes. A subsequent experiment using oral NMN at 400 mg/kg body weight was not associated with reduced sperm viability, oxidative stress, mitochondrial dysfunction or increased NAD+ in testes, suggesting that the negative impacts on sperm could be dependent on dose or mode of administration.

Evaluation of milt quality of Clarias anguillaris (Linnaeus, 1758) Broodstock fed varying inclusion levels of wild hibiscus Azanza garckeana pulp meal

2020 ◽  
Vol 34 (1) ◽  
pp. 23-31
Author(s):  
I.J. Ochokwu ◽  
M.O. Nwabunike ◽  
G.N. Udeh
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Control Diet ◽  
Feeding Trial ◽  
Inclusion Level ◽  
Fish Tank

Feeding trial was carried out to evaluate sperm quality of Clarias anguillaris broodstock fed varying inclusion levels of goron tula, Azanza garckeana pulp meal. The fish (mean weight 500g) were randomly distributed in concrete tanks (2 x 2 x 1.2m) at nine fish/tank in triplicates. Five isonitrogenous diets (40% CP) were formulated with the pulp meal incorporated at the rate of 0, 5, 10, 15, and 20% of the diet. Fish were fed at 3% of body weight for 56days. At the end of the feeding trial, results showed that dietary A. garckeana significantly (p<0.05) improved the milt quality of C. anguillaris. The broodfish fed 20% A. garckeana inclusion level had the highest (p<0.05) milt volume, sperm motility duration, testes lobe lengths, and whole sperm count while the least values of these variables were observed in group fed with the control diet (0% inclusion level). The findings conclusively revealed that increase in the level of A. garckeana pulp meal in the diet resulted into increase in sperm quality of C. anguillaris. Thus, the pulp meal can be freely utilized in catfish production in terms of sperm quality for mass fingerling production. Keywords: A. garckeana, Clarias anguillaris, sperm count, motility duration, milt volume.

Maternal protein restriction in pregnancy and/or lactation affects seminiferous tubule organization in male rat offspring

2012 ◽  
Vol 3 (5) ◽  
pp. 321-326 ◽  
Author(s):  
G. L. Rodríguez-González ◽  
R. M. Vigueras-Villaseñor ◽  
S. Millán ◽  
N. Moran ◽  
R. Trejo ◽  
...  
Keyword(s):  
Body Weight ◽  
Germ Cell ◽  
Control Diet ◽  
Protein Restriction ◽  
Male Offspring ◽  
Male Rat ◽  
Testis Weight ◽  
Germ Cell Differentiation ◽  
Maternal Protein Restriction ◽  
Cell Proliferation And Differentiation

Maternal protein restriction (MPR) during pregnancy impaired the reproduction of male offspring. We investigated, during the first wave of spermatogenesis, whether MPR exerts deleterious effects on germ cell proliferation and differentiation, as well as androgen receptor (AR) protein expression, which was used as a marker for Sertoli cell (SC) maturation. At the beginning of pregnancy (day 0), dams were fed a control diet (C: 20% casein) or a restricted isocaloric diet (R: 10% casein). After birth, four groups were established: CC, RR, CR and RC (first letter diet during pregnancy and second during lactation). Male offspring were studied at postnatal days 14, 21 and 36. At birth, pup body weight was unchanged. Body weight and testis weight were reduced in RR and CR groups at all ages evaluated. MPR delayed the germinal epithelium development at all ages evaluated. On performing Western blot and immunohistochemistry, AR expression was found to be lower in the three restricted groups. The results suggest that MPR during pregnancy and/or lactation delays SC maturation and germ cell differentiation, and affects intratubular organization. These changes might be responsible for the lower fertility rate at older ages.

Sex-specific effects of neonatal oral sucrose treatment on growth and liver choline and glucocorticoid metabolism in adulthood

2021 ◽  
Author(s):  
Cynthia Yamilka Ramírez-Contreras ◽  
Arya E Mehran ◽  
Melody Salehzadeh ◽  
Ei-Xia Mussai ◽  
Joshua W Miller ◽  
...  
Keyword(s):  
Body Weight ◽  
Preterm Infants ◽  
Serum Insulin ◽  
Control Diet ◽  
Male Mice ◽  
Long Term Effects ◽  
Female Mice ◽  
Specific Effects ◽  
Oral Sucrose

Hospitalized preterm infants experience painful medical procedures. Oral sucrose is the non‑pharmacological standard of care for minor procedural pain relief. Infants are treated with numerous doses of sucrose, raising concerns about potential long-term effects. The objective of this study was to determine the long‑term effects of neonatal oral sucrose treatment on growth and liver metabolism in a mouse model. Neonatal female and male mice were randomly assigned to one of two oral treatments (n=7‑10 mice/group/sex): sterile water or sucrose. Pups were treated 10 times/day for the first six days of life with 0.2mg/g body weight of respective treatments (24% solution; 1‑4μl/dose) to mimic what is given to preterm infants. Mice were weaned at age 3 weeks onto a control diet and fed until age 16 weeks. Sucrose-treated female and male mice gained less weight during the treatment period and were smaller at weaning than water-treated mice (p≤0.05); no effect of sucrose treatment on body weight was observed at adulthood. However, adult sucrose-treated female mice had smaller tibias and lower serum insulin-like growth factor-1 than adult water-treated female mice (p≤0.05); these effects were not observed in males. Lower liver S-adenosylmethionine, phosphocholine, and glycerophosphocholine were observed in adult sucrose-treated compared to water-treated female and male mice (p≤0.05). Sucrose-treated female, but not male, mice had lower liver free choline and higher liver betaine compared to water-treated female mice (p<0.01). Our findings suggest that repeated neonatal sucrose treatment has long-term sex-specific effects on growth and liver methionine and choline metabolism.

Adult exercise effects on oxidative stress and reproductive programming in male offspring of obese rats

2015 ◽  
Vol 308 (3) ◽  
pp. R219-R225 ◽  
Author(s):  
Mery Santos ◽  
Guadalupe L. Rodríguez-González ◽  
Carlos Ibáñez ◽  
Claudia C. Vega ◽  
Peter W. Nathanielsz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Maternal Obesity ◽  
Exercise Intervention ◽  
Sperm Quality ◽  
Average Distance ◽  
High Energy ◽  
Adverse Outcomes ◽  
Male Offspring ◽  
Oxidative Stress Biomarkers ◽  
Adiposity Index

Exercise improves health but few data are available regarding benefits of exercise in offspring exposed to developmental programming. There is currently a worldwide epidemic of obesity. Obesity in pregnant women predisposes offspring to obesity. Maternal obesity has well documented effects on offspring reproduction. Few studies address ability of offspring exercise to reduce adverse outcomes. We observed increased oxidative stress and impaired sperm function in rat offspring of obese mothers. We hypothesized that regular offspring exercise reverses adverse effects of maternal obesity on offspring sperm quality and fertility. Female Wistar rats ate chow (C) or high-energy, obesogenic diet (MO) from weaning through lactation, bred at postnatal day (PND) 120, and ate their pregnancy diet until weaning. All offspring ate C diet from weaning. Five male offspring (different litters) ran on a wheel for 15 min, 5 times/week from PND 330 to 450 and were euthanized at PND 450. Average distance run per session was lower in MO offspring who had higher body weight, adiposity index, and gonadal fat and showed increases in testicular oxidative stress biomarkers. Sperm from MO offspring had reduced antioxidant enzyme activity, lower sperm quality, and fertility. Exercise in MO offspring decreased testicular oxidative stress, increased sperm antioxidant activity and sperm quality, and improved fertility. Exercise intervention has beneficial effects on adiposity index, gonadal fat, oxidative stress markers, sperm quality, and fertility. Thus regular physical exercise in male MO offspring recuperates key male reproductive functions even at advanced age: it's never too late.

scholarly journals Brain-Restricted Inhibition of IL-6 Trans-Signaling Mildly Affects Metabolic Consequences of Maternal Obesity in Male Offspring

Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 3735
Author(s):  
Saida Breuer ◽  
Philipp Kasper ◽  
Christina Vohlen ◽  
Ruth Janoschek ◽  
Thorben Hoffmann ◽  
...  
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Transgenic Mice ◽  
Maternal Obesity ◽  
Male Offspring ◽  
Soluble Form ◽  
Standard Diet ◽  
Low Grade ◽  
Maternal And Newborn

Maternal obesity greatly affects next generations, elevating obesity risk in the offspring through perinatal programming and flawed maternal and newborn nutrition. The exact underlying mechanisms are poorly understood. Interleukin-6 (IL-6) mediates its effects through a membrane-bound receptor or by trans-signaling (tS), which can be inhibited by the soluble form of the co-receptor gp130 (sgp130). As IL-6 tS mediates western-style diet (WSD) effects via chronic low-grade inflammation (LGI) and LGI is an important mediator in brain–adipose tissue communication, this study aims at determining the effects of maternal obesity in a transgenic mouse model of brain-restricted IL-6tS inhibition (GFAPsgp130) on offspring’s short- and long-term body composition and epigonadal white adipose tissue (egWAT) metabolism. Female wild type (WT) or transgenic mice were fed either standard diet (SD) or WSD pregestationally, during gestation, and lactation. Male offspring received SD from postnatal day (P)21 to P56 and were metabolically challenged with WSD from P56 to P120. At P21, offspring from WT and transgenic dams that were fed WSD displayed increased body weight and egWAT mass, while glucose tolerance testing showed the strongest impairment in GFAPsgp130WSD offspring. Simultaneously, egWAT proteome reveals a characteristic egWAT expression pattern in offspring as a result of maternal conditions. IL-6tS inhibition in transgenic mice was in tendency associated with lower body weight in dams on SD and their respective offspring but blunted by the WSD. In conclusion, maternal nutrition affects offspring’s body weight and egWAT metabolism predominantly independent of IL-6tS inhibition, emphasizing the importance of maternal and newborn nutrition for long-term offspring health.

scholarly journals Effect of Maternal Obesity and Preconceptional Weight Loss on Male and Female Offspring Metabolism and Olfactory Performance in Mice

Nutrients ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 948 ◽  
Author(s):  
Polina E. Panchenko ◽  
Marie-Christine Lacroix ◽  
Mélanie Jouin ◽  
Sarah Voisin ◽  
Karine Badonnel ◽  
...  
Keyword(s):  
Weight Loss ◽  
Maternal Obesity ◽  
Control Diet ◽  
Female Offspring ◽  
Adverse Outcomes ◽  
Male Offspring ◽  
Obese Women ◽  
Expression Of Genes ◽  
Obese Mother ◽  
Genes Encoding

According to the “developmental origins of health and disease” (DOHaD) concept, maternal obesity predisposes the offspring to non-communicable diseases in adulthood. While a preconceptional weight loss (WL) is recommended for obese women, its benefits on the offspring have been poorly addressed. We evaluated whether preconceptional WL was able to reverse the adverse effects of maternal obesity in a mouse model, exhibiting a modification of foetal growth and of the expression of genes encoding epigenetic modifiers in liver and placenta. We tracked metabolic and olfactory behavioural trajectories of offspring born to control, obese or WL mothers. After weaning, the offspring were either put on a control diet (CD) or a high-fat (HFD). After only few weeks of HFD, the offspring developed obesity, metabolic alterations and olfactory impairments, independently of maternal context. However, male offspring born to obese mother gained even more weight under HFD than their counterparts born to lean mothers. Preconceptional WL normalized the offspring metabolic phenotypes but had unexpected effects on olfactory performance: a reduction in olfactory sensitivity, along with a lack of fasting-induced, olfactory-based motivation. Our results confirm the benefits of maternal preconceptional WL for male offspring metabolic health but highlight some possible adverse outcomes on olfactory-based behaviours.

scholarly journals Effects of Bene (Pistacia atlantica) on Histopathology of Testis, Sperm Chromatin Quality and Stress Oxidative in Busulfan-Induced Infertile Mice

2019 ◽  
Vol 25 (1) ◽  
pp. 24-30
Author(s):  
Hojat Norasteh ◽  
Shabnam Mohammadi ◽  
Mohammad Reza Nikravesh ◽  
Samaneh Broumand ◽  
Farimah Beheshti
Keyword(s):  
Oxidative Stress ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Parameters ◽  
Sperm Chromatin ◽  
Control Group ◽  
Mice Model ◽  
Pistacia Atlantica ◽  
Stress Oxidative ◽  
And Oxidative Stress

Background: Some plants stimulate spermatogenesis and increase fertility, while some cause spermatogenesis arrest. So far, the effects of bene (Pistacia atlantica) on male fertility have not been studied. The aim of this study was to investigate the effects of bene on sperm parameters, testicular histopathology, sperm quality, and oxidative stress in busulfan-induced infertile mice. Methods: Thirty-five male BALB/c mice were randomly assigned to control, sham, busulfan, bene, and bene + busulfan groups. The busulfan group received 10 mg/kg as a single dose and intraperitoneally. The bene group received pellets containing 10% of bene. Another group received 10 mg/kg busulfan and was fed with pellet containing 10% bene. Then, sperms, sperm chromatin quality, testicular histopathology, and oxidative stress levels were studied on the 35th day of the experiment. Results: Busulfan injection resulted in a significant reduction in sperm parameters compared to the control group (p<0.001); it decreased after bene administration (p<0.001). In addition, in the group treated with bene, the sperm count with damaged DNA was reduced and the level of malondialdehyde decreased compared to the busulfan group. A significant increase was observed in the mean level of superoxide dismutase and catalase enzymes in the bene + busulfan group compared to the busulfan group (p<0.001). The histopathological improvement of the testis was observed in the bene + busulfan group. Conclusion: The administration of 10 mg/kg of bene powder for 35 days reduced the oxidative stress, improved testicular histopathology, sperm chromatin quality, and sperm parameters in the infertile mice model.

scholarly journals L-Tryptophan Metabolism in Pregnant Mice Fed a High L-Tryptophan Diet and the Effect on Maternal, Placental, and Fetal Growth

2013 ◽  
Vol 6 ◽  
pp. IJTR.S12715 ◽  
Author(s):  
Ai Tsuji ◽  
Chifumi Nakata ◽  
Mitsue Sano ◽  
Tsutomu Fukuwatari ◽  
Katsumi Shibata
Keyword(s):  
Body Weight ◽  
Fetal Growth ◽  
Control Diet ◽  
Tryptophan Metabolism ◽  
Casein Diet ◽  
Female Mice ◽  
Pregnant Mice ◽  
Diet Control ◽  
The Relationship

Excess L-tryptophan (L-Trp) in the diet decreases fetal body weight. However, the relationship between L-Trp concentration and its effects on maternal, placental, and fetal growth are not well-understood. We investigated the effects of excess L-Trp intake on maternal, placental, and fetal growth. Female mice were fed a 20% casein diet (control diet) or control diet plus 2% or 5% L-Trp during gestation. Pup weights did not differ between the control (L-Trp intake: 0.04 g/kg body weight (BW)/day) and 2% L-Trp groups (L-Trp intake: 3.3 g/kg BW/day), but were significantly lower in the 5% L-Trp group (L-Trp intake: 7.0 g/kg BW/day) than in the control and 2% L-Trp groups. These results show that less than 3.3 g/kg BW/day L-Trp intake in pregnant mice during gestation does not affect fetal growth or L-Trp homeostasis in the placenta or fetus.

scholarly journals Diet and Nutritional Factors in Male (In)fertility—Underestimated Factors

2020 ◽  
Vol 9 (5) ◽  
pp. 1400
Author(s):  
Kinga Skoracka ◽  
Piotr Eder ◽  
Liliana Łykowska-Szuber ◽  
Agnieszka Dobrowolska ◽  
Iwona Krela-Kaźmierczak
Keyword(s):  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Therapeutic Interventions ◽  
Sperm Dna Fragmentation ◽  
Omega 3 ◽  
Unhealthy Lifestyle ◽  
Balanced Diet ◽  
Sperm Dna ◽  
Mineral Components

In up to 50% of cases, infertility issues stem solely from the male. According to some data, the quality of human semen has deteriorated by 50%–60% over the last 40 years. A high-fat diet and obesity, resulting from an unhealthy lifestyle, affects the structure of spermatozoa, but also the development of offspring and their health in later stages of life. In obese individuals, disorders on the hypothalamic-pituitary-gonadal axis are observed, as well as elevated oestrogen levels with a simultaneous decrease in testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) levels. Healthy dietary models clearly correlate with better sperm quality and a smaller risk of abnormalities in parameters such as sperm count, sperm concentration and motility, and lower sperm DNA fragmentation. Apart from mineral components such as zinc and selenium, the role of omega-3 fatty acids and antioxidant vitamins should be emphasized, since their action will be primarily based on the minimization of oxidative stress and the inflammation process. Additionally, the incorporation of carnitine supplements and coenzyme Q10 in therapeutic interventions also seems promising. Therefore, it is advisable to have a varied and balanced diet based on vegetables and fruit, fish and seafood, nuts, seeds, whole-grain products, poultry, and low-fat dairy products.

scholarly journals Prenatal Choline Supplementation during High-Fat Feeding Improves Long-Term Blood Glucose Control in Male Mouse Offspring

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 144
Author(s):  
Hunter W. Korsmo ◽  
Kaydine Edwards ◽  
Bhoomi Dave ◽  
Chauntelle Jack-Roberts ◽  
Huanling Yu ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Maternal Obesity ◽  
Control Diet ◽  
Blood Glucose Control ◽  
Female Offspring ◽  
Male Offspring ◽  
High Fat ◽  
Obesogenic Diet ◽  
Dietary Treatments

Maternal obesity increases the risk of metabolic dysregulation in rodent offspring, especially when offspring are exposed to a high-fat (HF), obesogenic diet later in life. We previously demonstrated that maternal choline supplementation (MCS) in HF-fed mouse dams during gestation prevents fetal overgrowth and excess adiposity. In this study, we examined the long-term metabolic influence of MCS. C57BL/6J mice were fed a HF diet with or without choline supplementation prior to and during gestation. After weaning, their pups were exposed to either a HF or control diet for 6 weeks before measurements. Prenatal and post-weaning dietary treatments led to sexually dimorphic responses. In male offspring, while post-weaning HF led to impaired fasting glucose and worse glucose tolerance (p < 0.05), MCS in HF dams (HFCS) attenuated these changes. HFCS (versus maternal normal fat control) appeared to improve metabolic functioning of visceral adipose tissue during post-weaning HF feeding, preventing the elevation in leptin and increasing (p < 0.05) mRNA expression of insulin receptor substrate 1 (Irs1) that promotes peripheral insulin signaling in male offspring. In contrast, MCS had minimal effects on metabolic outcomes of female offspring. In conclusion, MCS during HF feeding in mice improves long-term blood glucose homeostasis in male offspring when they are faced with a postnatal obesogenic environment.

Sign in / Sign up

Export Citation Format

Share Document