scholarly journals Cytogenetic analysis of genetic damage in young individuals exposed to Chronic Mechanical Irritation in the lateral border of the tongue due to sharp teeth – A novel study.

2020 ◽  
Author(s):  
Thuckanickenpalayam Ragunathan Yoithapprabhunath ◽  
Nalliappan Ganapathy ◽  
Seeni Renugadevi ◽  
Jalaludheen MisbhaRafath ◽  
Vishnuvarthan Aparna ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Early Detection ◽  
Cytogenetic Analysis ◽  
Invasive Technique ◽  
Control Group ◽  
Study Group ◽  
Genetic Damage ◽  
Healthy Individuals ◽  
Lateral Border ◽  
Mechanical Irritation

Background. Chronic inflammation is estimated to contribute to approximately 25% of human cancers. Inflammation can be induced by chronic mechanical irritation which can lead to oral pre-cancer and oral cancer. Though many studies have been conducted to identify the genetic damage in oral cancer or dysplastic stages, no study has been completed so far on early detection of genetic damage in healthy individuals presenting with sharp teeth in contact with the lateral border of the tongue. Objective. The study is aimed at analyzing  the genetic damage (micronuclei) in healthy individuals with sharp teeth in contact with the lateral border of the tongue causing chronic mechanical irritation. Methods. The study group comprised of 75 clinically healthy individuals with sharp teeth in contact with the lateral border of the tongue and 25 clinically healthy individuals with normal teeth in contact with the lateral border of the tongue as a control group. Brush samples of epithelial cells were collected, then spread over clean glass slide and fixed in 100% alcohol, stained with rapid PAP and analyzed under the light microscope. The exfoliated cells were examined to detect micronuclei. Results. Micronuclei frequency was found to be increased in the study group where sharp teeth were in contact with the lateral border of the tongue of healthy individuals without deleterious habits such as usage of tobacco in smoking/smokeless form, areca nut chewing or alcohol consumption. Conclusions. Cytogenetic analysis is a simple and scantly invasive technique allowing clinicians the early detection of DNA damage in patients with sharp teeth and subsequently preventing carcinogenesis by proper treatment and follow-up.

Effect of Anterolateral Thigh Perforator Flap Repair on the Appearance, Language and Masticatory Function of Oral Cancer Patients after Radical Surgery

2021 ◽  
Vol 7 (5) ◽  
pp. 4277-4285
Author(s):  
Fengchun Hou ◽  
Di Wang ◽  
Tao Jiang
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Myocutaneous Flap ◽  
Perforator Flap ◽  
Pectoralis Major ◽  
Pectoralis Major Myocutaneous Flap ◽  
Control Group ◽  
Study Group ◽  
Masticatory Function ◽  
Oral Cancer Patients

Purpose: The purpose was to explore the application effect of anterolateral thigh perforator flap (ALTP) and pectoralis major myocutaneous flap on repairing the tissuedefects after oral cancer surgery. Methods: 100 oral cancer patients treated in our hospital from January 2018 to December 2020 were selected as the research objects. After both groups of patients received extended resection of tumors, neck lymph node dissection and other routine treatment, the control group was repaired by pectoralis major myocutaneous flap while the study group was repaired by ALTP to compare the flap survival rate, language, masticatory function and other related indicators between the two groups. Results: The flap harvesting time and defect repair time in the control group were shorter than those in the study group (P<0.05); The language, masticatory function and swallowing function in both groups after treatment were significantly better than those before treatment (P<0.05); The total incidence of complications in the study group was significantly lower than that in the control group (P<0.05); After treatment, the quality of life scores in both groups were significantly higher than those before treatment (P<0.05). Conclusion: Both ALTP and pectoralis major myocutaneous flap can be used to repair the tissue defects after radical resection of oral cancer, which can effective improve the postoperative appearance, language and masticatory function of oral cancer patients. However, pectoralis major myocutaneous flap has easier operation and shorter operation time while ALTP can significantly reduce the incidence of postoperative complications with higher safety.

scholarly journals STUDY OF THE LEVEL OF FATTY ACIDS IN ERYTHROCYTE MEMBRANES AND SERUM OF PATIENTS WITH COLORECTAL CANCER IN NOVOSIBIRSK

2018 ◽  
Vol 5 (2) ◽  
pp. 50-61 ◽  
Author(s):  
M. V. Kruchinina ◽  
V. N. Kruchinin ◽  
Ya. I. Prudnikova ◽  
A. A. Gromov ◽  
M. V. Shashkov ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Erythrocyte Membranes ◽  
Control Group ◽  
Study Group ◽  
Omega 3 ◽  
Healthy Individuals ◽  
Internal Organs ◽  
Omega 6

The objectiveis to measure the level of fatty acids in erythrocyte membranes and serum of patients with colorectal cancer.Materials and methods.The study group included 100 patients with diagnosed colorectal cancer (57 men and 43 women). The control group included 24 reasonably healthy people (14 men and 10 women) matched for age and sex, without malignant cancers or manifested pathology of the internal organs.Results.Decreased levels of saturated, monounsaturated fatty acids and increased levels of polyunsaturated fatty acids (PUFAs) in erythrocyte membranes and serum (p <0.0001–0.05) were observed. The levels of omega-3 PUFAs in colorectal cancer exceeded the levels in healthy individuals both in erythrocyte membranes and in serum; for omega-6 PUFAs only a trend was observed. At the same time, the ratio of omega-6/omega-3 PUFAs in colorectal cancer was lower than in control (p <0.0001–0.002). The state of erythrocyte membranes more significantly and for more parameters characterized differences between the groups than serum. The most discriminating parameters between patients with colorectal cancer and healthy individuals both in erythrocyte membranes and serum were the levels of C20:2;11,14 (eicosadienoic), C20:3;8,11,14 (dihomo-γ-linolenic), C20:4;5,8,11,14 (eicosatetraenoic, arachidonic), C22:5;7,10,13,16,19 (docosapentaenoic), and C22:6;4,7,10,13,16,19 (docosahexaenoic) PUFAs.

scholarly journals Repeated short cycles of nonsteroidal anti-inflammatory drugs and kidney injury in patients with spinal degenerative-dystrophic diseases

2018 ◽  
Vol 12 (3) ◽  
pp. 94-97
Author(s):  
D. M. Bichurina ◽  
I. Z. Gaydukova ◽  
D. А. Patrikeeva ◽  
A. P. Rebrov
Keyword(s):  
Back Pain ◽  
Kidney Injury ◽  
Control Group ◽  
Study Group ◽  
Creatinine Ratio ◽  
Healthy Individuals ◽  
Group A ◽  
Inflammatory Drugs ◽  
The Mean ◽  
Anti Inflammatory

Objective: to evaluate kidney function in patients with spinal degenerative-dystrophic diseases (SDDDs) who take nonsteroidal anti-inflammatory drugs (NSAIDs) as repeated short cycles of treatment for severe back pain.Patients and methods. The investigation enrolled 97 patients with SDDDs who took NSAIDs for back pain (a study group). A control group consisted of sexand age-matched healthy individuals who had not used NSAIDs within the past year (n=40). Glomerular filtration rate (GFR) was estimated using the CKD-EPI equation and markers of kidney injury (albuminuria and globulinuria) were measured.Results. In the study group, GFR was decreased to <90 ml/min/1.73 m2 in 61 (62.9%) patients, to <60 ml/min/1.73 m2 in 11 (11.3%); the mean GFR was 77.5 [68.0; 89.0] ml/min/1.73 m2; in the control group, a decline in GFR to 89–60 ml/min/1.73 m2 was recorded in 35 (62.5%) cases; this indicator was >90 ml/min/1.73 m2 in the remaining 15 (37.5%) cases; the mean GFR was 82.5 [70.8; 90.0] ml/min/1.73 m2 (p≥0.05 for all pairwise comparisons). A decrease in GFR to <60 ml/min/1.73 m2 was found in 11 (11.3%) patients in the study group and in nobody in the control group (p=0.026). Elevated albuminuria was noted in 74 (76.3%) patients with SDDDs and in 9 (22.5%) healthy individuals (p<0.05). Albumin/creatinine ratio was 57.1 [33.8; 82.4] mg/g in the study group and 25.0 [17.5; 32.9] mg/g in the control group (p<0.0001). Increased globulinuria was established in all the patients with SDDDs and only in 3 (7.5%) healthy examinees. Globulin/creatinine ratio was 134.7 [77.5; 197.7] mg/g in the study group and 12.9 [0.5; 18.1] mg/g in the control group (p<0.0001).Conclusion. A decline in GFR to <60 ml/min/1.73 m2 was more often seen in the patients taking NSAIDs for spine pain caused by SDDDs than in the healthy individuals. In case of comparable GFR, the level of kidney injury markers was significantly higher in the study group than that in the control group, which suggests that patients with SDDDs who take NSAIDs have subclinical tubulointerstitial and glomerular changes.

The Acute Effect of Talocrural Joint Mobilization on Dorsiflexor Muscle Strength in Healthy Individuals: A Randomized Controlled Single-Blind Study

2019 ◽  
Vol 28 (6) ◽  
pp. 601-605 ◽  
Author(s):  
Ufuk Ersoy ◽  
Umut Ziya Kocak ◽  
Ezgi Unuvar ◽  
Bayram Unver
Keyword(s):  
Muscle Strength ◽  
Acute Effect ◽  
Control Group ◽  
Study Group ◽  
Healthy Individuals ◽  
Talocrural Joint ◽  
Joint Mobilization ◽  
Randomized Controlled ◽  
Single Blind ◽  
Ankle Dorsiflexor

Context: Mobilization has been used for enhancing muscle strength. Objective: The aim of this study was to investigate the acute effect of talocrural joint mobilization on ankle dorsiflexor muscle strength in healthy individuals, which has not yet been studied. Design: Randomized controlled single-blind study. Setting: University laboratory. Participants: Forty-eight healthy individuals. Interventions: Maitland grade III (study group) versus Maitland grade I (control group) mobilizations. Main Outcome Measures: Muscle strength measurements were performed using a handheld dynamometer at baseline, immediately after the mobilization, and 30 minutes after mobilization. Results: At baseline, the physical characteristics and muscular strength were similar in both groups (P > .05). According to Friedman analysis, a significant difference was detected following the mobilization in the study group (P < .001), and while the muscle strength at immediately after the mobilization and at 30 minutes after mobilization was significantly higher than baseline (P < .001), no significant differences were observed between 30 minutes after mobilization and immediately after the mobilization (P = .17). However, no significant changes were detected in the control group. The study group was found superior to the control group in terms of muscle strength differences following the mobilization (P < .001). Conclusion: The ankle dorsiflexor muscle strength might be increased by performing Maitland grade III mobilization, and this increase might be preserved for 30 minutes, while Maitland grade I mobilization did not lead to such an improvement in healthy individuals.

scholarly journals The 400-Meter Walk Test to Evaluate Walking Performance between Diabetics and Healthy Females

1970 ◽  
Vol 2 (01) ◽  
pp. 84-95
Author(s):  
Ronald Pakasi ◽  
Angela BM Tulaar ◽  
Sarwono Waspadji ◽  
Corrie Wawolumaya
Keyword(s):  
Walking Speed ◽  
Control Group ◽  
Study Group ◽  
Walk Test ◽  
Healthy Individuals ◽  
Warm Up ◽  
Significant Difference ◽  
Walking Performance ◽  
Female Subjects

Objective: to evaluate walking performance in female with type 2 diabetes mellitus (DM2) with the 400-meters walk test (400-MWT) compared to healthy individuals.Methods: two groups of female subjects with DM2 and healthy individuals were matched by the age. The 400-MWT parameters to be compared were walking speed (WS) and predicted maximum oxygenconsumption (pVO2max). Baseline examination included body mass index (BMI), random blood glucose (RBG), and ankle-brachial index (ABI). All subjects performed 2 minutes warm up before thetest. Heart rate (HR) was recorded every 30 seconds, and blood pressure (BP) was measured before warm up and within 60 seconds after test. The test was performed twice on a different day.Results: Nineteen subjects on each group participated in the study. The mean WS was significantly different (p<0.0001) between the study group (1.26 + 0.19 and 1.31 + 0.17 m/s) and the control group(1.70 + 0.20 and 1.78 + 0.24 m/s) for the first and second tests respectively. There was a significant difference of mean pVO2max (p<0.0001) between the study group (17.22 + 2.94 and 17.99 + 2.36 ml/kg/min) and the control group (23.68 + 3.79 and 24.44 + 3.74 ml/kg/min).Conclusion: the 400-MWT demonstrated lower walking performance in female subjects with DM2 compared to healthy individuals.Keywords: Type 2 DM, healthy individuals, 400 meter walk test, walking speed, VO2max.

scholarly journals Analysis of genetic damage in individuals exposed to Chronic Mechanical Irritation (CMI) in the lateral border of the tongue due to Cusp of Carabelli (CoC) –A Cytogenetic study

2020 ◽  
Vol 01 (01) ◽  
pp. 01-04
Author(s):  
Thuckanickenpalayam Ragunathan Yoithap prabhunath ◽  
Seeni Renugadevi ◽  
Kenniyan Kumar Sri Chinthu ◽  
Shanmugam Mohanapriya ◽  
Krubakar Rachel SarahVinodhini ◽  
...  
Keyword(s):  
Cytogenetic Study ◽  
Genetic Damage ◽  
Lateral Border ◽  
Mechanical Irritation

scholarly journals Importance of early detection of lung cancers with initial paraneoplastic manifestations

2010 ◽  
Vol 138 (9-10) ◽  
pp. 595-599
Author(s):  
Vera Vukic ◽  
Vesna Skodric-Trifunovic ◽  
Branislav Gvozdenovic ◽  
Dragana Jovanovic ◽  
Miodrag Zunic ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Early Detection ◽  
Working Group ◽  
Small Cell ◽  
Control Group ◽  
Study Group ◽  
Small Cell Lung ◽  
Lung Cancers ◽  
Significant Difference ◽  
Disease Stages

Introduction. Lung cancers are mostly detected in the developed clinical stages, with clearly manifested pulmonary, extrapulmonary or metastatic manifestations. In the early disease stages, radiographic and clinical manifestations may be absent or mimicked. Objective. The aim of this study was the timely detection of early pulmonary, extrapulmonary and paraneoplastic manifestations of lung cancers in order to apply the most appropriate treatment protocols. Methods. We examined 230 patients with lung cancer, among them 125 of the working study group with minimal pulmonary and/or initial paraneoplastic symptoms, and 105 patients in the control group with clear tumour manifestations. Results. The symptom analysis revealed a statistically significantly lesser presence of the respiratory symptoms in the working study group (68%) in comparison with the control group of patients (97%) (c2=29.996; p<0.001). The analysis of radiographic presentations of lung cancer showed that there were significantly more patients with normal findings in the working group (6.4%) than in the control group - 1.9% (p>0.05), and a positive bronchoscopic finding of the centrally localized tumour (mainly right upper lobe) was confirmed in patients of both groups with normal radiographic findings. The number of diagnosed patients in earlier clinical disease stages (I, II, IIIa) with better prognosis in non-small cell lung cancer was significantly higher (c2=19.149; p<0.001) in the working group (71.1%) in comparison with the control group (38.1%). Small cell lung cancer was more frequently diagnosed in the stage of limited disease in the working (80%) than in the control group (38.1%) (c2=10.039; p<0.05). With regard to treatment administration, there is a statistically significant difference (c2=4.013; p=0.0452) in the frequency between the use of chemotherapy and highly significant difference (c2=22.044; p<0.001) in the frequency of use of surgical treatment in the working group in comparison with the control group - both chemotherapy and surgery treatment were more frequent in the patients of the working group. Conclusion. Recognizing the initial pulmonary, extrapulmonary or paraneoplastic manifestations as well as performing diagnostic procedures in due time represent the most important guidelines in early detection and the most efficacious therapeutic choice in lung cancer.

scholarly journals Impact of oligohydramnios on maternal and perinatal outcome: a comparative study

2018 ◽  
Vol 7 (8) ◽  
pp. 3205
Author(s):  
Sreelakshmi U. ◽  
Tushara Bindu ◽  
Subhashini T.
Keyword(s):  
Early Detection ◽  
Intrauterine Growth Restriction ◽  
Perinatal Outcome ◽  
Control Group ◽  
Study Group ◽  
Amniotic Fluid Index ◽  
Appropriate Treatment ◽  
Umbilical Artery Doppler ◽  
And Control ◽  
The Impact

Background: Oligohydramnios has got significant impact on perinatal outcome and maternal morbidity. Therefore, early detection and its management will help in reduction of perinatal morbidity and mortality, decreased operative interventions. Hence, the present study is undertaken to study the impact of oligohydromnios on perinatal-maternal outcome.Methods: The present study was prospective comparative observational study conducted in the Department of Obstetrics and Gynaecology, Mallareddy Narayana Multispecialty Hospital, reputed teaching hospital from January 2015 to August 2017. The women were divided into study and control groups based on AFI, 100 cases were selected in each group.Results: Out of the 200 women, included in the present study, in study group AFI <5 cm was present in 51% of patients and AFI 5-8 cm in 49% of patients. Doppler abnormalities found in study group (n = 33). 32% of the patients in study group had non-reactive NST while in control group 8%. Caesarean section was performed in 70% of cases in study group as compared to 9% in control group. Foetal distress was the most common indication for LSCS. There were no perinatal deaths in this study.Conclusions: In this study amniotic fluid index of ≤5 cm was commonly associated with increased cesarean section rates, intrauterine growth restriction, Non-reactive NST and abnormal umbilical artery Doppler velocimetry studies. Hence, every case of oligohydramnios needs to be evaluated carefully, early detection and initiation of appropriate treatment and treat the cause if possible.

Presence of Bcr-Abl Positive Cells in Peripheral Blood Leukocytes of Immunosuppressed Solid Organ Transplant (SOT) Recipients

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 3191-3191
Author(s):  
Philipp D. le Coutre ◽  
Petra Reinke ◽  
Ruth Neuhaus ◽  
Ralf Trappe ◽  
Frauke Ringel ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Case Reports ◽  
Control Group ◽  
Study Group ◽  
Solid Organ ◽  
Healthy Individuals ◽  
Peripheral Blood Leukocytes ◽  
Rt Pcr ◽  
Blood Leukocytes ◽  
Female Ratio

Abstract In chronic myeloid leukemia (CML) the p210 BCR-ABL protein, generated by a t(9;22)(q34;q11) translocation, is the underlying mechanism of leukemogenesis. Although the presence of p210 BCR-ABL is normally restricted to CML patients (pts), previous reports demonstrated low levels of bcr-abl transcripts in healthy individuals that may be controlled by an intact immune system (Bose et al. Blood92:3362, 1998). Interestingly, several articles reported cases of CML occurring in pts after SOT (Pelloso et al. Leukemia Res29:353, 2005). Therefore, immunosuppressed SOT recipients should represent an optimal population to investigate the frequency of bcr-abl transcripts in non-leukemic individuals in order to address a potential impact of immunosuppression on the presence of bcr-abl transcripts. This possibility was investigated by studying peripheral blood leukocytes for the presence of bcr-abl transcripts in a total of 201 individuals of whom 100 were SOT recipients (n=50 kidney, n=46 liver, n=3 heart, n=1 heart-lung) and 101 were control individuals (n=87 patients with renal failure, n=14 healthy individuals). The male to female ratio in the study group was 54:46 (median age: 55.38 years, range: 22–83) and matched the control group (median age: 59.8 years, range: 32–96). Included in the study group were 13 pts with post transplant malignancies who were previously treated with standard chemotherapeutic regimens. Immune suppressive drugs given to all SOT recipients included steroids (90%), cyclosporine A (69%), azathioprine (22%), tacrolimus (69%), sirolimus (44%), mycophenolic acid (63%) and others (37%). For the detection of the bcr-abl transcript we used a nested reverse transcriptase-polymerase chain reaction (RT-PCR) assay that is routinely used in our institution. All samples were tested at least twice. In 5/100 immunosuppressed pts at least 1 of 2 RT-PCR products was bcr-abl positive in the second round amplification. This rate significantly exceeded the control group that was completely bcr-abl negative (0/101 p = 0.0242). Of the 5 bcr-abl positive pts, 3 were liver transplant and 2 were kidney transplant recipients. The latency in this group (interval between transplantation and bcr-abl PCR) was at 58.4 months (range: 24-135 months) and shorter when compared to the latency in the remaining study group (104.8 months; range 1 – 369). Additionally, all samples were tested for both the pml-rara and aml1-eto transcripts that are detectable in subsets of pts with acute myeloid leukaemia but none of the samples tested positive for these transcripts. Our findings are extended by three case reports of SOT recipients (2 kidney, 1 liver) who developed CML in a total of 2088 transplantations in our centre in 9 years. In these three pts (2 males, 1 female), CML occurred at 84.3 months (range: 32–183) following SOT. In summary our data show: 1. The presence of bcr-abl transcripts in immunosuppressed non-leukemic SOT pts and the absence of bcr-abl transcripts in healthy normals when tested with routine PCR protocols. 2. The absence of pml-rara and aml1-eto transcripts in both the study and control group. And 3. the occurrence of CML in three patients following SOT suggesting a higher frequency of CML in this population.

Early outcomes of mitral valvuloplasty by minimally invasive surgery or sternotomy

2020 ◽  
Vol 28 (7) ◽  
pp. 398-403
Author(s):  
Huan-lei Huang ◽  
Qian Yan ◽  
Xu-jing Xie ◽  
Kan Zhou ◽  
Biao-chuan He ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Median Sternotomy ◽  
Invasive Technique ◽  
Control Group ◽  
Study Group ◽  
Transfusion Rate ◽  
Invasive Approach ◽  
Thoracoscopic Approach ◽  
Mitral Valvuloplasty

Background Disagreement exists regarding methods for repair of the mitral valve. We compared early outcomes of mitral valvuloplasty by a minimally invasive technique and by a median sternotomy. Methods The data of 507 patients (mean age 47.9 ± 15.2 years) undergoing mitral valvuloplasty from January 2015 to June 2018 were analyzed retrospectively. In the study group ( n = 225), mitral valvuloplasty via a totally thoracoscopic approach was performed by a single surgeon. In the control group ( n = 282), mitral valvuloplasty via the traditional median sternotomy was carried out by other cardiac surgeons in our hospital. Clinical data, surgical results, and follow-up findings in the two groups were comparatively analyzed. Results In the study group, the blood transfusion rate (5.3% vs. 20.9%, p < 0.05) and incidences of poor wound healing (0 vs. 5.3%, p < 0.05) and respiratory tract infection (4.4% vs. 16.3%, p < 0.05) were lower, and postoperative hospitalization was shorter (5.9 ± 4.0 vs. 10.7 ± 8.4 days, p < 0.05). Within 30 days after surgery, no patient died in the study group while one died in the control group. The duration of follow-up was 12–36 months (mean 22.9 ± 8.8 months). During follow-up, there were 1 and 0 cases of redo surgery and 1 and 3 deaths in the study group and control group, respectively. Conclusion Mitral valvuloplasty via a minimally invasive approach is superior to the traditional median sternotomy in terms of early outcomes, especially when performed by experienced surgeons.

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