scholarly journals Transport Characteristics of Plasma Membranes of PK-15 Passaged Cells

2021 ◽  
Vol 31 (1) ◽  
pp. 14-22
Author(s):  
Larisa Kuleshova ◽  
◽  
Igor Kovalenko ◽  
Svetlana Kovalenko ◽  
Tetiana Tsibulko ◽  
...  
Keyword(s):  
Experimental Data ◽  
Plasma Membranes ◽  
Volume Ratio ◽  
Relative Volume ◽  
Water Molecules ◽  
Analytical Evaluation ◽  
Passive Transport ◽  
Cell Parameters ◽  
Permeability Coefficients ◽  
Optimal Duration

The values of permeability coefficients to water molecules and cryoprotectants are demanded to select the optimal duration of exposure of cells in cryoprotective media at the stage of their preparation for cryopreservation, as well as to find optimal cooling and warming rates during the freeze-thawing of cell suspensions. The necessary numerical values of such cell parameters as the osmotically inactive volume α and the surface-area-to-volume ratio γ were obtained for the analytical evaluation of the permeability coefficients of the PK-15 cells’ plasma membranes using physico-mathematical modelling. The permeability coefficients kp of the plasma membranes of PK-15 cells to 1,2-PD, EG, DMSO and glycerol cryoprotectants molecules, as well as the filtration coefficients Lp to water molecules at temperatures of 25, 15 and 5°C were determined by approximating the experimental data of the change in relative volume of cells on exposure time in the studied solutions by theoretical curves calculated on the basis of physical and mathematical model of passive transport of water and permeable substances under the condition of their maximum coincidence. The value of the activation energy of the transmembrane transfer of molecules of these substances is calculated

On the structural organization of biological pumps and channels

1984 ◽  
Vol 42 ◽  
pp. 138-141
Author(s):  
G. Zampighi ◽  
M. Kreman
Keyword(s):  
Active Transport ◽  
Transport System ◽  
Plasma Membranes ◽  
Transport Proteins ◽  
Molecular Organization ◽  
Passive Transport ◽  
Concentration Gradients ◽  
Cell Functions ◽  
Natural Concentration ◽  
Active Transport System

The plasma membranes of most animal cells contain transport proteins which function to provide passageways for the transported species across essentially impermeable lipid bilayers. The channel is a passive transport system which allows the movement of ions and low molecular weight molecules along their concentration gradients. The pump is an active transport system and can translocate cations against their natural concentration gradients. The actions and interplay of these two kinds of transport proteins control crucial cell functions such as active transport, excitability and cell communication. In this paper, we will describe and compare several features of the molecular organization of pumps and channels. As an example of an active transport system, we will discuss the structure of the sodium and potassium ion-activated triphosphatase [(Na+ +K+)-ATPase] and as an example of a passive transport system, the communicating channel of gap junctions and lens junctions.

Structural Study of a new Compound Based on Acid 1,4-Cyclohexanedicarboxylic (1,4-CDC)

2010 ◽  
Vol 6 (1) ◽  
pp. 891-896
Author(s):  
Manel Halouani ◽  
M. Dammak ◽  
N. Audebrand ◽  
L. Ktari
Keyword(s):  
Aqueous Solution ◽  
Water Molecules ◽  
Carboxyl Group ◽  
X Ray Diffraction ◽  
Compound I ◽  
Unit Cell Parameters ◽  
Monoclinic System ◽  
X Ray ◽  
Cell Parameters ◽  
Cyclohexanedicarboxylic Acid

One nickel 1,4-cyclohexanedicarboxylate coordination polymers, Ni2 [(O10C6H4)(COO)2].2H2O  (I), was hydrothermally synthesized from an aqueous solution of Ni (NO3)2.6H2O, (1,4-CDC) (1,4-CDC = 1,4-cyclohexanedicarboxylic acid) and tetramethylammonium nitrate. Compound (I) crystallizes in the monoclinic system with the C2/m space group. The unit cell parameters are a = 20.1160 (16) Å, b = 9.9387 (10) Å, c = 6.3672 (6) Å, β = 97.007 (3) (°), V= 1263.5 (2) (Å3) and Dx= 1.751g/cm3. The refinement converged into R= 0.036 and RW = 0.092. The structure, determined by single crystal X-ray diffraction, consists of two nickel atoms Ni (1) and Ni (2). Lots of ways of which is surrounded by six oxygen atoms, a carboxyl group and two water molecules.

scholarly journals Influence of the Porosity of Polymer Foams on the Performances of Capacitive Flexible Pressure Sensors

Sensors ◽  
2019 ◽  
Vol 19 (9) ◽  
pp. 1968 ◽  
Author(s):  
Sylvie Bilent ◽  
Thi Hong Nhung Dinh ◽  
Emile Martincic ◽  
Pierre-Yves Joubert
Keyword(s):  
Experimental Data ◽  
Linear Model ◽  
Dielectric Material ◽  
Pressure Sensors ◽  
Volume Ratio ◽  
Measurement Range ◽  
Polymer Foams ◽  
First Order ◽  
Non Linear ◽  
Flexible Pressure Sensors

This paper reports on the study of microporous polydimethylsiloxane (PDMS) foams as a highly deformable dielectric material used in the composition of flexible capacitive pressure sensors dedicated to wearable use. A fabrication process allowing the porosity of the foams to be adjusted was proposed and the fabricated foams were characterized. Then, elementary capacitive pressure sensors (15 × 15 mm2 square shaped electrodes) were elaborated with fabricated foams (5 mm or 10 mm thick) and were electromechanically characterized. Since the sensor responses under load are strongly non-linear, a behavioral non-linear model (first order exponential) was proposed, adjusted to the experimental data, and used to objectively estimate the sensor performances in terms of sensitivity and measurement range. The main conclusions of this study are that the porosity of the PDMS foams can be adjusted through the sugar:PDMS volume ratio and the size of sugar crystals used to fabricate the foams. Additionally, the porosity of the foams significantly modified the sensor performances. Indeed, compared to bulk PDMS sensors of the same size, the sensitivity of porous PDMS sensors could be multiplied by a factor up to 100 (the sensitivity is 0.14 %.kPa−1 for a bulk PDMS sensor and up to 13.7 %.kPa−1 for a porous PDMS sensor of the same dimensions), while the measurement range was reduced from a factor of 2 to 3 (from 594 kPa for a bulk PDMS sensor down to between 255 and 177 kPa for a PDMS foam sensor of the same dimensions, according to the porosity). This study opens the way to the design and fabrication of wearable flexible pressure sensors with adjustable performances through the control of the porosity of the fabricated PDMS foams.

Systematic ab initio study of the compressibility of silicate garnets

2001 ◽  
Vol 57 (2) ◽  
pp. 163-177 ◽  
Author(s):  
V. Milman ◽  
E. V. Akhmatskaya ◽  
R. H. Nobes ◽  
B. Winkler ◽  
C. J. Pickard ◽  
...  
Keyword(s):  
Experimental Data ◽  
Density Functional ◽  
Structural Parameters ◽  
Applied Pressure ◽  
Basis Set ◽  
Ionic Radii ◽  
Cell Parameters ◽  
The Density Functional Theory ◽  
Dodecahedral Site ◽  
Compression Mechanism

The structural properties of the silicate garnets andradite, Ca3Fe2Si3O12, uvarovite, Ca3Cr2Si3O12, knorringite, Mg3Cr2Si3O12, goldmanite, Ca3V2Si3O12, blythite, Mn^{2+}_3Mn^{3+}_2Si3O12, skiagite, Fe^{2+}_3Fe^{3+}_2Si3O12, calderite, Mn^{2+}_3Fe^{3+}_2Si3O12, and khoharite, Mg3Fe^{3+}_2Si3O12, have been investigated with a quantum-mechanical model as a function of applied pressure. The study has been performed with the density functional theory code CASTEP, which uses pseudopotentials and a plane-wave basis set. All structural parameters have been optimized. The calculated static geometries (cell parameters, internal coordinates of atoms and bond lengths), bulk moduli and their pressure derivatives are in good agreement with the experimental data available. Predictions are made for those cases where no experimental data have been reported. The data clearly indicate that the elastic properties of all silicate garnets are dominated by the compressibility of the dodecahedral site. The compression mechanism is found to be based on a bending of the angle between the centers of the SiO4 tetrahedra and the adjacent octahedra, as in the aluminosilicate garnets. An analysis of the relationship between ionic radii of the cations and the compressibility of silicate garnets is presented.

Heterobimetallische 3d-4f-Komplexe mit Bis(1,2-dithiooxalato)nickelat(II) als planarem Brückenbaustein / Heterobimetallic 3d-4f-Complexes with Bis(1,2-dithiooxalato)nickelate(II) as Planar Bridging Block

2005 ◽  
Vol 60 (11) ◽  
pp. 1149-1157 ◽  
Author(s):  
Matthias Siebold ◽  
Alexandra Kelling ◽  
Uwe Schilde ◽  
Peter Strauch
Keyword(s):  
Room Temperature ◽  
Magnetic Moments ◽  
Lanthanide Ions ◽  
Water Molecules ◽  
Unit Cell Parameters ◽  
Ionic Radii ◽  
X Ray ◽  
Cell Parameters ◽  
Heterobimetallic Complexes ◽  
The Individual

Planar bis(1,2-dithiooxalato)nickelates(II) react in aqueous solutions of lanthanide ions to form pentanuclear, heterobimetallic complexes of the general composition [{Ln(H2O)n}2- {Ni(dto)2}3]・xH2O (Ln = Y3+, La3+, Ce3+, Pr3+, Nd3+, Sm3+, Eu3+, Gd3+, Tb3+, Dy3+, Ho3+, Er3+, Tm3+, Yb3+, Lu3+; n = 4 or 5; x = 9 - 12). With [{Nd(H2O)5}2{Ni(S2C2O2)2}3]・xH2O (x = 10 - 12) (1) and [{Er(H2O)4}2{Ni(S2C2O2)2}3]・xH2O (x = 9 - 10) (2) we were able to isolate two complexes of this series as single crystals, which were characterized by X-ray structure analysis. Depending on the individual ionic radii of the lanthanide ions, the compounds crystallize in two different crystal systems with the following unit cell parameters: 1, monoclinic in P21/c with a = 11.3987(13), b = 11.4878(8), c = 20.823(2) Å , β = 98.907(9)° and Z = 2; 2, triclinic in P1̅ with a = 10.5091(6), b = 11.0604(6), c = 11.2823(6) Å , α = 107.899(4)°, β = 91.436(4)°, γ = 112.918(4)° and Z = 1. The channels and cavities appearing in the packing of the molecules are occupied by uncoordinated water molecules. High magnetic moments up to 14.65 BM./f.u. have been observed at room temperature due to the combined moments of the individual lanthanide ions.

scholarly journals Molecular Dynamics Modellization and Simulation of Water Diffusion through Plant Cutin

1999 ◽  
Vol 54 (11) ◽  
pp. 896-902 ◽  
Author(s):  
Antonio Matas ◽  
Antonio Heredia
Keyword(s):  
Experimental Data ◽  
Molecular Dynamics ◽  
Structural Information ◽  
Md Simulations ◽  
Water Molecules ◽  
Plant Cuticle ◽  
X Ray Diffraction ◽  
Cross Link ◽  
X Ray ◽  
Good Agreement

Abstract A theoretical molecular modelling study has been conducted for cutin, the biopolyester that forms the main structural component of the plant cuticle. Molecular dynamics (MD) simulations, extended over several ten picoseconds, suggests that cutin is a moderately flexible netting with motional constraints mainly located at the cross-link sites of functional ester groups. This study also gives structural information essentially in accordance with previously reported experimental data, obtained from X -ray diffraction and nuclear magnetic resonance experiments. MD calculations were also performed to simulate the diffusion of water mole­cules through the cutin biopolymer. The theoretical analysis gives evidence that water perme­ation proceedes by a “hopping mechanism”. Coefficients for the diffusion of the water molecules in cutin were obtained from their mean-square displacements yielding values in good agreement with experimental data.

Empirical Models for a Screw Expander Based on Experimental Data From Organic Rankine Cycle System Testing

2014 ◽  
Vol 136 (6) ◽  
Author(s):  
Vamshi Krishna Avadhanula ◽  
Chuen-Sen Lin
Keyword(s):  
Experimental Data ◽  
Power Output ◽  
Pressure Ratio ◽  
Organic Rankine Cycle ◽  
Volume Ratio ◽  
Rankine Cycle ◽  
Empirical Models ◽  
Working Fluid ◽  
Work Output ◽  
Experimental Values

The screw expander discussed in this work was part of a 50 kW organic Rankine cycle (ORC) system. The ORC was tested under different conditions in heat source and heat sink. In conjunction with collecting data for the ORC system, experimental data were also collected for the individual components of the ORC, viz. evaporator, preheater, screw expander, working fluid pump, and condenser. Experimental data for the screw expander were used to develop the two empirical models discussed in this paper for estimating screw expander performance. As the physical parameters of the screw expander discussed in this article are not known, a “black-box” approach was followed to estimate screw expander power output, based on expander inlet and outlet pressure and temperature data. Refrigerant R245fa was used as the working fluid in the ORC. The experimental data showed that the screw expander had ranges of pressure ratio (2.70 to 6.54), volume ratio (2.54 to 6.20), and power output (10 to 51.5 kW). Of the two empirical models, the first model is based on the polytropic expansion process, in which an expression for the polytropic exponent is found by applying regression curve-fitting analysis as a function of the expander pressure ratio and volume ratio. In the second model, an expression for screw expander work output is found by applying regression curve-fitting analysis as a function of the expander isentropic work output. The predicted screw expander power output using the polytropic exponent model was within ±10% of experimental values; the predicted screw expander power output using the isentropic work output model was within ±7.5% of experimental values.

Modelling of the Waste Form Behaviour in a Wet Near-Surface Repository Site Over Extended Time Periods

2001 ◽  
Author(s):  
Michael I. Ojovan ◽  
Natalia V. Ojóvan ◽  
Irene V. Startceva ◽  
Zoja I. Golubeva ◽  
Alexander S. Barinov
Keyword(s):  
Experimental Data ◽  
Surface Area ◽  
Salt Content ◽  
Volume Ratio ◽  
Waste Glass ◽  
Near Surface ◽  
Waste Forms ◽  
Time Periods ◽  
Radionuclide Release

Abstract A mathematical model was used to predict radionuclide release from bitumen and glass waste forms over extended time periods. To calculate some model parameters, we used experimental data derived from 12yr field tests with six borosilicate waste glass blocks (each ∼30 kg in weight) and a bitumen block (310 kg), containing real intermediate-level NPP operational waste (NaNO3, 86 wt.% of a dry salt content; 137Cs, 82% of the radioactive inventory). Specific radioactivities of the glass material containing 35 wt.% waste oxides were βtot(90Sr+90Y), 3.74×106 Bq/kg, and αtot(239Pu), 1.3×104Bq/kg. The bitumen block with ∼31 wt.% salt content and βtot(90Sr+90Y), 4.0·106 Bq/kg, and αtot(239Pu), 3.0×103 Bq/kg was manufactured on base of a hard bitumen BN-IV. Tests with the waste forms were performed under saturated conditions of an experimental near-surface repository with a free access of groundwater to the waste blocks through a covering of host loamy soil and backfill of coarse sand. The way used to quantify the amount of leached radioactivity was to measure the volume and radioactivity concentrations of contacting groundwater. In the model, radionuclide release from the waste glass is assumed to be controlled by the processes of diffusion limited ion exchange and glass network dissolution. The mechanism of radionuclide release from the bitumen matrix is believed to remain the same throughout the long-term storage period, except for the initial stage when an enhanced leaching from the surface layer occurs. This long-term release is assumed to be controlled by diffusion of radionuclides through the bitumen matrix. So, identical formulae were applied to calculate the values of leached radioactivity fractions for two waste forms. Radioactivity release curves were plotted for field data and calculation results. For both waste forms, there was good agreement between the modelled and available experimental data. According to the modelling results, fmax = 2.3×10−3% of the initial radioactivity will release from the waste glass into the environment within a proposed institutional control period of 300 years under conditions of the near-surface repository and in the absence of additional engineered barriers. For the bitumen block and the same 300-yr period, the total (maximum) leached radioactivity fraction will be fmax = 4.2×10−3%. The main result of the modelling and experimental studies concerning the leaching behaviour of the bituminised and vitrified waste materials is that the fractional radioactivity release for two waste forms is on the same order of magnitude. Numerical release values per a unit of a surface area to volume ratio are also rather close for two waste forms (exposed surface area to volume ratio for the bitumen block is 2 to 4 times greater then for the glass).

The occurrence of coarse-grained massive tilleyite in the Redcap Creek magmatic skarn, North Queensland

1985 ◽  
Vol 49 (350) ◽  
pp. 71-75 ◽  
Author(s):  
M. J. Rubenach ◽  
C. Cuff
Keyword(s):  
Experimental Data ◽  
Mass Transfer ◽  
Close Agreement ◽  
Igneous Rocks ◽  
Coarse Grained ◽  
Reverse Direction ◽  
Unit Cell Parameters ◽  
Cell Parameters ◽  
Activity Diagrams ◽  
Grey Colour

AbstractEndoskarns formed where a swarm of diorite dykes have intruded calcite marble at Redcap Creek include an inner melilite-dominated, a wollastonite-dominated, and an outer massive tilleyite zone in contact with marble. The massive tilleyite is unusual in that it is coarse-grained (prisms 2–15 cm in length) and its deep grey colour contrasts with the lighter coloured varieties described elsewhere. A chemical analysis gives a formula close to ideal, with only minor substitution of Al, Ti, and Mg. Refined unit cell parameters are in close agreement with those quoted in the literature. The skarns have clearly formed by transport of Si, Mg, Fe, Al, and Ti from the igneous rocks, and Ca in the reverse direction from the marble. Activity diagrams derived from experimental data are most useful in interpreting the zonal sequence of endoskarns, and preliminary results suggest mass transfer at low Xco2 and temperature of the order of 800°C or higher for the formation of the massive tilleyite.

scholarly journals Tunable Two-Layer Dual-Band Metamaterial with Negative Modulus

Materials ◽  
2019 ◽  
Vol 12 (19) ◽  
pp. 3229
Author(s):  
Limei Hao ◽  
Meiling Men ◽  
Yazhe Wang ◽  
Jiayu Ji ◽  
Xiaole Yan ◽  
...  
Keyword(s):  
Mass Density ◽  
Hollow Spheres ◽  
Volume Ratio ◽  
Blue Shift ◽  
Relative Volume ◽  
Hole Diameter ◽  
Dual Band ◽  
Resonant Frequencies ◽  
In Series ◽  
Simulation Results

A tunable dual-band acoustic metamaterial (AM) with nested two-layer split hollow spheres (TLSHSs) is presented here, which was achieved by adjusting the hole diameter and the ratio of the two layers’ volumes. This work comprises theoretical and numerical studies. Based on sound-force analogy (SFA), TLSHSs can be considered equivalent to a model of two spring oscillators in series. The equations of two resonant frequencies were derived, which precisely provided the relation between two resonant frequencies and the hole diameter as well as the ratio of the two layers’ volumes. The analytical formulas and simulation results by the finite element method (FEM) showed that there were two resonant frequencies for the TLSHSs, and their dynamic modulus became negative near the resonant frequencies. As the the diameter of two holes increased, both of the resonant frequencies underwent a blue shift. As the relative volume ratio increased, both of the resonant frequencies underwent a red shift. The calculation and simulation results were in good agreement. This kind of precisely controllable dual-band AM with negative modulus can easily be coupled to other structures with negative mass density, thereby achieving a double-negative AM in an expected frequency range.

Sign in / Sign up

Export Citation Format

Share Document