scholarly journals Hepatoprotective and nephroprotective effects of Trigonella foenum-graecum L. (Fenugreek) seed extract against sodium nitrite toxicity in rats

2019 ◽  
Vol 6 (5) ◽  
pp. 3142-3150 ◽  
Author(s):  
Gözde ATİLA USLU ◽  
Hamit USLU ◽  
Yasemen ADALI

Introduction: Feeding habits and environmental factors may rival genetic susceptibility as etiological factors related to various cancers. Humans are continuously exposed to many synthetic food additives, one of which is sodium nitrite (NaNO2). There is a direct correlation between increases in consumption of nitrite-treated products and incidence of tissue damage, hepatotoxicity, nephrotoxicity and some types of cancer. The objective of this study was to investigate the protective effects of Trigonella foenum-graecum (TFG) on NaNO2-induced hepatotoxicity and nephrotoxicity. Methods: Forty rats were randomly assigned (10 per group) to control (physiological saline solution), TFG (150 mg/kg/day), NaNO2 (80 mg/kg/day), and NaNO2+TFG (80 mg/kg/day + 150 mg/kg/day) groups. This group was offered TFG seed extract two hours before NaNO2. At the end of three months, the rats were decapitated, and blood, kidney and liver tissues were removed. Results: Three months of oral administration of NaNO2 increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, and pro-inflammatory cytokine levels in the liver and kidney tissues [except for liver Interleukin 1 alpha (IL-1a)] of rats. Serum AST, ALT, urea, creatinine, liver IL-6, and kidney tumor necrosis factor-a (TNF-a), IL-6, IL-1a levels significantly decreased in the NaNO2+TFG group compared to the NaNO2 group. Pathological examinations, it was determined show that exogenously administered TFG could alleviate the effects of NaNO2 hepatotoxicity and nephrotoxicity. Conclusions: Our results suggest that exogenous TFG mitigates NaNO2-administration induced hepatotoxicity and nephrotoxicity. TFG extract exerted antioxidative and anti-inflammatory effects, and played a significant role in preventing hepatic and renal damage induced by chronic NaNO2 administration.  

2012 ◽  
Vol 6 (38) ◽  
pp. 5119-5127 ◽  
Author(s):  
Dinakaran Vasudevan ◽  
Sridharan Subhashree ◽  
Periyasamy Latha ◽  
Sudha Rani Sankaramoorthy

Author(s):  
Imad M Al-ani ◽  
Soraya Ismail ◽  
Khin M Maung ◽  
Pakeer Oothuman ◽  
Sinan Mohammed Abdullah Al-mahmood

  Objective: Tamarind (Tamarindus indica) has been used as a medical plant for treating many human and animal diseases and widely used as a traditional herbal medicine for the treatment of snake bites. The objective of the study is to investigate whether tamarind seed extract (TSE) has neutralization activity on an adverse histological reaction against venoms of the King Cobra.Methods: A total of 20 healthy mature male mice were randomly divided into 4 groups with 5 mice in each. The control group was injected with 1 ml of normal saline. The second group was injected subcutaneously with a single dose of 24.96 μg/20 g King Cobra venom (KCV) solution. The third group was injected with the same dose of KCV solution and 10 mg/20 g of TSE. The fourth group was injected with the same dose of KCV solution and 15 mg/20 g TSE solution. The animals were sacrificed after 24 hrs of injection of the solution. Fragments of muscle, kidney, and liver were fixed in 10% neutral buffered formalin and processed for light microscopical studies.Results: The result showed that TSE reduced the histopathological changes induced by the KCV in the muscles, livers, and kidneys, and the improvement was proportional to the applied dose of the TSE indicating that TSE prevents adverse histological changes in the muscle, liver, and kidney.Conclusion: The present study demonstrated that TSE reduced the histopathological changes in the muscle, liver, and kidney induced by KCV in mice.


2012 ◽  
Vol 8 (2) ◽  
pp. 1555-1565 ◽  
Author(s):  
Najla Hfaiedh ◽  
Sabah Dhibi ◽  
Sakria Mbarki ◽  
Jean-Claude Murat ◽  
Abdel Fattah Elfeki

Protective effects of Fenugreek seeds (Trigonella foenum graecum L), added in the diet, upon oxidative stress and dysfunctions in kidney, thyroid and liver of alloxan-diabetic rats were investigated.In our study, the alloxan-induced diabetes triggered 1) increased levels of glucose, total cholesterol and triglycerides in blood, 2) increased activities of alkaline phosphatase and transaminases in blood, 3) increased levels of creatinine, urea and protein in blood, 4) a decreased level of TSH and an increased level of free thyroxin in plasma.In addition, an oxidative stress, evidenced by an increase of lipids peroxidation level and superoxide dismutase activity associated with a decrease of glutathione peroxidase and catalase activities in hepatic and renal tissues, was observed.When Fenugreek seeds powder (100g/kg) was added in the food for 30 days, all this parameters were significantly shifted to more normal values.In conclusion, fenugreek seeds powder displays beneficial effects upon hepatotoxicity, nephropathy, thyroid dysfunction and oxidative stress in alloxan-diabetic rats. This property could be attributed to the presence of antioxidant components, such as complex polysaccharides and phenolic acids, as confirmed by analyses. 


2019 ◽  
Vol 29 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Lucie Widowati ◽  
Ani Isnawati ◽  
Sukmayati Alegantina ◽  
Fifi Retiaty

Abstract The proportion of stunting in children under 5 years old in Indonesia was approximately 30,8% in 2018. One of the things that is presumably related to the occurrence of stunting is lack of nutrition that can be attributed to exclusive breastfeeding. Fenugreek seeds have properties as laktogoga and moringa leaves in addition to having efficacy as lactagoga, also have advantage in nutritional content, particularly high protein content, groups of minerals and vitamins. The aim of the study was to assess the potential of the formula fenugreek seed extract (Trigonella foenum-graecum L) and moringa leaves (Moringa oleifera Lamk.) as a supplement to release milk while at the same having nutrient content. Methods using the test weighting method in pregnant female rats by measuring the volume of milk taken by baby rats. Test were carried out on five groups of mother rats namely groups than were given : fenugreek and moringa extract (1: 1) with different doses I, II and III; comparison group (moloco) and control group (distilled water). Each mother fed five rats, provision of extract was carried out starting the first day after the mother gave birth, and measurement were take on 6th, 11th, 16th to 21st day The volume of milk consumed by five rats was counted as difference of body weight after and before breastfeeding. Moringa nutritional assessment is carried out using standard methods on, iron, calcium and potassium content; vitamin A and vitamin C content and protein level. Fenugreek-moringa extract (1: 1) dose of 30 mg/200g bb, can increase the volume of breast milk of female rat larger than the untreated group (p≤0.05). The cumulative weight gain of rat infants was greater than the control group (p≤0.05), according to the nutrients obtained from the fenugreek-moringa extract. Moringa leaves as a component of extract, have high nutritional value of minerals, vitamins and proteins and have advantages as lactogogum. Conclusion: The administration of moringa leaf and fenugreek seed extract (1: 1) dose of 30 mg / 200 g bb in breastfeeding female rats has a 2.4 times greater potential in increasing milk expenditure compared to breastfeeding female rats who did not get the same extract. Fenugreek-moringa extract has superior potential as laktagogum and at the same time a high nutritional supplement. Abstrak Proporsi kejadian stunting pada anak usia di bawah 5 tahun (balita) di Indonesia tercatat sekitar 30,8% pada tahun 2018. Salah satu hal yang diduga berkaitan dengan terjadinya stunting adalah gizi kurang yang dapat dikaitkan dengan pemberian air susu ibu (ASI) eksklusif. Biji klabet memiliki khasiat sebagai laktagoga dan daun kelor selain mempunyai khasiat sebagai laktagoga, juga mempunyai keunggulan pada kandungan gizinya, terutama kandungan protein yang tinggi, golongan mineral dan vitamin. Penelitian bertujuan untuk menilai potensi formula ekstrak biji klabet (Trigonella foenum-graecum L.) dan daun kelor (Moringa oleifera Lamk.) sebagai suplemen pelancar pengeluaran air susu sekaligus memiliki kandungan nutrisi yang tinggi. Metode menggunakan test weighting method pada tikus betina hamil, dengan cara mengukur volume air susu yang diminum oleh bayi tikus. Dilakukan uji pada lima kelompok induk tikus yaitu: kelompok yang diberi ekstrak klabet dan kelor (1:1) dengan dosis I, II dan III yang berbeda; kelompok pembanding (moloco) dan kelompok kontrol (akuades). Tiap induk tikus menyusui lima ekor anak tikus. Pemberian bahan uji dilakukan mulai hari pertama setelah induk tikus melahirkan, dan pengukuran dilakukan pada hari ke-6, 11, 16 sampai 21. Volume air susu yang diminum lima ekor anak tikus dihitung sebagai selisih berat badan sesudah dan sebelum disusui induknya. Penilaian gizi kelor dilakukan menggunakan metoda standar, terhadap kandungan mineral besi, kalsium dan kalium; kandungan vitamin A dan vitamin C, serta kadar protein. Hasil penelitian menunjukkan ekstrak klabet-kelor (1:1) dosis 30 mg/200g bb, dapat memperbanyak pengeluaran volume air susu induk tikus lebih besar dari kelompok yang tidak diberi perlakuan (p≤0,05). Pertambahan berat kumulatif bayi tikus, lebih besar dibandingkan kelompok kontrol (p≤0,05), sesuai dengan gizi yang didapat dari ekstrak klabet-kelor. Daun kelor sebagai komponen ekstrak, mempunyai nilai gizi mineral, vitamin, dan protein yang tinggi serta memiliki keunggulan sebagai laktogogum. Kesimpulan penelitian adalah pemberian ekstrak biji klabet-daun kelor (1:1) dosis 30 mg/200g bb pada tikus betina menyusui mempunyai potensi 2,4 kali lebih besar dalam meningkatkan pengeluaran air susu dibandingkan kelompok tikus betina menyusui yang tidak mendapatkan ekstrak yang sama. Ekstrak biji klabet-kelor mempunyai potensi unggul sebagai laktagogum dan sekaligus suplemen dengan gizi tinggi.


2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Imad M. Al-Ani ◽  
Soraya Ismail ◽  
Khin Maung Maung ◽  
Pakeer Oothuman

Introduction: The objective of the present study is to investigate protective effects of the tamarind seed extract on the adverse histological reaction of venoms of the King Cobra. Methods: Twenty healthy, mature male mice were randomly divided into 4 groups with 5 mice in each. The control group (C) was injected with 1 ml of normal saline. The second group (V) was injected subcutaneously with a single dose of 24.96µg/20g King Cobra venom (KCV) solution. The third group (TV1) was injected with the same dose of KCV solution and 10mg/20g of Tamarind seed extract (TSE). The fourth group (TV2) was injected with the same dose of KCV solution and 15mg/20g TSE solution. The animals were sacrificed after 24 hours of injection of the solution. Fragments of muscle, kidney and liver were fixed in 10% buffered formalin, and processed for light microscopical studies. Results: Mice of group V, treated with KCV solution showed severe degeneration, disorganization and myonecrosis in the muscle fibers. The liver revealed degenerative changes in most of the hepatic lobules and dilatation in the central veins and the sinusoids. The kidney revealed shrunken glomeruli, dilatation of Bowman capsule and degenerative changes in the renal tubules. Treatment with TSE reduced the histopathological changes induced by the King Cobra venom in the muscles, livers and kidneys and the improvement were proportional to the applied dose of the TSE. Conclusions: The observation is indicating that tamarind seed extract prevents adverse histological changes in the muscle, liver and kidney.


2012 ◽  
Vol 31 (04) ◽  
pp. 423-430 ◽  
Author(s):  
Najla Hfaiedh ◽  
Hichem Alimi ◽  
Jean-Claude Murat ◽  
 Abdelfattah Elfeki

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