scholarly journals Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

2021 ◽  
Vol 12 (2) ◽  
pp. 315-320
Author(s):  
S. О. Slobodian ◽  
B. V. Gutyj ◽  
L. M. Darmohray ◽  
M. G. Povoznikov

Prolonged ingress of heavy metals into the organisms of animals, even in low concentrations, causes a number of toxic impacts, affecting various organs and systems. The objective of this study was to research parameters of enzymatic and non-enzymatic links of the system of antioxidant protection of the organism of young cattle in the condition of lead-cadmium load and the effect of Metisevit Plus feed additive. Studies were conducted on the basis of the Private Agricultural Company “Ukraina”, located in Dubrovytsia district of Rivne Oblast, using 12 six-month old bulls of Ukrainian Black Pied Dairy breed divided into two groups, each consisting of 6 animals. Bulls of the control group received the standard diet. The bulls of the experimental group were fed with Metisevit Plus feed additive in the dose of 0.5 g/kg of feed. The contents of lead and cadmium in feed at the enterprise were determined to be high. According to the conducted studies, it was determined that in conditions of cadmium and lead load, there is a decrease in activity of glutathione link of the system of antioxidant protection of the organism of bulls. Feeding the bulls of the experimental group with Metisevit Plus feed additive for 30 days led to increase in the levels of non-enzymatic and enzymatic activities of the glutathione system. Significant increase in the level of reduced glutathione was recorded on days 30 and 40 of the experiment. Consumption of Metisevit Plus by the animals of the experimental group promoted increase in the activity of catalase and superoxide dismutase starting on the 20th day of the experiment. On days 30 and 40 of the experiment, the activity of the studied enzymes ranged within the physiological values. Therefore, the studies conducted on the bulls indicate that in the conditions of cadmium and lead load the feed additive Metisevit Plus fed to the bulls activated the system of antioxidant protection by increasing the levels of enzymatic and non-enzymatic links. When feeding bulls of the experimental group with Metisevit Plus feed additive, we determined significant decreases in diene conjugates and TBA-active products in their blood starting already on the 10th day of the experiment. On days 20 and 30 of the experiment, the level of diene conjugates in the blood of animals of the experimental group decreased by 14.5% and 24.0%. Efficiency of the feed additive may be explained by the effect of its constituents on the main pathogenic links of the pathological process caused by cadmium and lead. As indicated in the studies, liquidation of the syndrome of endogenous intoxication restores the functional condition of cellular membranes of the liver, protein-synthesizing function of the liver, increases the antioxidant status of the organisms of bulls. The studies we conducted confirm the expedience of using feed additive Metisevit Plus to prevent lead-cadmium toxicosis.

2019 ◽  
Vol 21 (95) ◽  
pp. 98-101
Author(s):  
I. S. Varkholiak ◽  
B. V. Gutyj

The aim of this study was to investigate the effect of Bendamin on the antioxidant status of rats in experimental doxorubicin-induced cardiomyopathy. The studies were performed on white sexually mature young male of Vistar line rats weighing 180–200 g, which were kept on a standard diet of the Institute Vivarium of the State Research Institute of Veterinary Drugs and Feed Additives. Animals were divided into 3 groups of 6 animals in each: control group – intact animals; experimental group R1, in which animals were simulated with doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks; experimental group R2, in which animals were injected with the drug “Bendamin” at a dose of 20 mg/kg after injection of doxorubicin. The data obtained indicate that the introduction into the experimental animals of the experimental group of doxorubicin is accompanied by intensification in the myocardium of the processes of free radical oxidation. The increase in diene conjugates was found to be 32.7% and TBK-active products increased by 37.6% compared to the control group of rats. Doxorubicin was also found to cause inhibition of the glutathione system of the animal's antioxidant protection after administration to rats. After administration of the drug “Bendamin”, rats of the second experimental group were suppressed with lipid peroxidation under the conditions of doxorubicin intoxication. In the rats of the second experimental group, the level of intermediates and end products is likely to decrease, so in the rat myocardial homogenate the level of diene conjugates decreased by 16.8% and the level of TBA-active products by 20.8% compared to the first experimental group. who had clinical signs of cardiomyopathy caused by doxorubicin administration. The use of the drug “Bendamin” in rats of the second experimental group contributed to the enhancement of the enzymatic and non-enzymatic element of the antioxidant system, protecting the structural and functional integrity of cell bioembranes. The results of the studies enrich the pharmacological characteristics of the drug “Bendamin”, indicate its sufficiently clear protective effect on the myocardium in experimental doxorubicin cardiomyopathy and is a convincing proof of the feasibility of the drug in veterinary practice.


2020 ◽  
Vol 22 (100) ◽  
pp. 54-59
Author(s):  
S. О. Slobodian ◽  
B. V. Gutyj ◽  
D. F. Gufriy ◽  
P. S. Hnativ ◽  
S. D. Murska

In the conditions of modern technogenic pollution of the environment, environmental problems, improving the quality of livestock products and their food safety is an important and urgent issue today. Pollution of the environment with heavy metals due to man-made activities of the population has led to a number of problems of agricultural production. The aim of the study was to investigate the effect of sodium selenite and feed additive “Metisevit plus” on the protein-synthesizing function and functional state of the liver of rats in lead-cadmium intoxication. It was found that the level of total protein in the blood of rats exposed to cadmium and lead was reduced. These changes were due to a decrease in albumin levels and an increase in globulin levels. Thus, hypoproteinemia resulting from cadmium and lead poisoning may indicate impaired hepatic protein synthesis. Transamination enzymes play a major role in the relationship between protein and carbohydrate metabolism, which is important for switching the oxidation of energy substrates from glucose to amino acid energy nutrition, which is extremely important under the influence of various environmental factors. The most active transaminases are aspartate aminotransferase and alanine aminotransferase. In the study of the activity of aminotransferases in the serum of rats treated with lead acetate and cadmium chloride, an increase in the activity of enzymes in all periods of the study. The highest activity of aminotransferases was on days 21 and 28 of the experiment in the control group of rats, which carried out the combined load of cadmium and lead. Thus, the load of the body of animals with cadmium and lead contributes to the violation of the functional state and protein-synthesizing function of the liver of animals of the experimental groups. With the introduction of sodium selenite into the body of rats, the activity of ALT and AST in the serum of the first experimental group was slightly reduced compared with the control group. However, the activity of these enzymes did not reach physiological values. When setting the feed additive “Metisevit plus” throughout the experiment, it was found that the activity of aminotransferases in the serum of rats of the second experimental group ranged within physiological values. This is due to the hepatoprotective effect of the components of the feed additive “Metisevit Plus”. In the study of protein-synthesizing function of rat liver in experimental lead-cadmium toxicosis, it was found that after application to rats of the first experimental group of sodium selenite on days 14, 21 and 28, the level of total protein in serum gradually increased, but did not reach physiological values. Using the feed supplement “Metisevit plus” rats of the second experimental group, found a slightly higher level of total protein in their blood, which on the 28th day of the experiment reached physiological values and, accordingly, amounted to 66.8 ± 1.99 g/l. It should be noted that when administering the feed additive “Metisevit plus” protein-synthesizing liver function and liver function of rats of the second experimental group were restored faster than when administering sodium selenite to the first experimental group.


REPORTS ◽  
2021 ◽  
Vol 2 (336) ◽  
pp. 13-17
Author(s):  
N. N. Kuzmina ◽  
O. Yu. Petrov ◽  
A. K. Karynbayev ◽  
A. S. Alentayev

The purpose of the research was to determine the feasibility and effectiveness of using a natural antioxidant of plant origin – dihydroquercetin (DHA), as a biologically active substance, in combination with the main standard diet of broiler chickens. Scientific and economic experience conducted in production conditions on broiler chickens of the "COBB-500" cross allowed us to objectively establish that the addition of the biologically active substance Dihydroquercetin to mixed feed contributes to the increase in meat productivity and safety of broiler chickens. Feeding of broiler chickens was carried out with full-fledged compound feeds, in accordance with the recommendations of the originator for this cross. In terms of energy nutrition and the content of nutrient active substances, they were the same and differed between the groups in the amount of the additive administered. Chickens of the 1st control group received only feed (basic diet), the 2nd experimental group – advanced antioxidant supplement Dihydroquercetin in the amount of 0.5 g per 100 g of feed, the 3rd – 0.75 g per 100 g of feed, and 4th – 1 g per 100 g of feed. The duration of the experiment was 40 days and to clarify the effectiveness of increasing the duration of the broiler fattening period, it was extended to 60 days. Broiler chickens were reared on the floor, temperature and light conditions, air humidity, feeding and watering front of the bird during the experiment corresponded to the recommended standards. As a result of the conducted studies on the effect of adding optimal levels of biologically active feed additive Dihydroquercetin to the diet of broiler chickens, it was found that during the growing period, a higher coefficient of live weight gain was established in broiler chickens of the 3rd and 4th experimental groups, whose live weight increased by 105.32 and 120.48 times by the age of 40 days, while in broiler chickens of the 2nd experimental group by 102.57 times and the lowest–in control group chickens only in 90.96 times. The selected feed additive can be considered as an alternative to the use of feed antibiotics in the feeding of broiler chickens in industrial poultry farming technology.


2021 ◽  
Vol 212 ◽  
pp. 188-192
Author(s):  
V.I. Kotarev ◽  
◽  
L.I. Denisenko ◽  
V.V. Shipilov ◽  
◽  
...  

The results of morpho-biochemical, immunological studies and data on the antioxidant status of young laying hens of Czech Dominant breed when including “Profort” feed additive in the ration are presented. The study was carried out in the conditions of the poultry farm of Krasnoye Podvorie farm in Belgorod region. The chickens were divided into two groups: experimental and control. Young birds of the control group received the main ration. As for the experimental group, “Profort” feed additive, which contained Bacilius megaterium B-4801 and Enterococus faecium l-35, was used at a dose of 0.5 kg / t in combination with the main ration. Blood samples for research were taken on 11, 21 and 42 days. In the course of the studies, a positive effect on morpho-biochemical, immunological parametres and parametres of antioxidant defense system of egg cross chicken blood when using “Profort” probiotic was revealed. A decrease of the content of monocytes, eosinophils, lymphocytes and pseudo-eosinophils was noted throughout the entire period of the study in blood of young birds of the experimental group, compared to the control group. The concentration of alpha-globulins in the blood of birds of the experimental group was higher throughout the entire period of the study relative to the parametres of the control group. The maximum difference in immunological parameters of the blood of birds of the experimental and control groups was achieved on the 42nd day of the study. So, lysozyme activity of blood serum of young laying hens of the experimental group was higher by 18.8%, and the content of CIC and general Ig - by 30.7% and 21.9%, respectively. A decrease of medium molecular peptide level in blood shows a positive effect on the antioxidant status of the organism


Author(s):  
T. V. Martyshuk ◽  
B. V. Gutyj

The purpose of the work was to investigate the effect of the feed supplement “Butaselmevit Plus” on the antioxidant status of the organism in rats under conditions of oxidative stress. The research was carried out on white, sexually-mature, young male rats of the Wistar line, with a body weight of 180–200 g, which was kept on a standard diet of the Institute vivarium of the State Scientific-Research Control Institute of Veterinary Preparations and Feed Supplements. The animals were divided into three groups of 20 animals in each: 1st group (K) intact animals; Group 2 (R1) – rats, affected with tetrachloromethane; Group 3 (R2) – rats, affected with tetrachloromethane and used as a feed additive “Butaselmevit-Plus”. The feed supplement “Butaselmevit-Plus” includes the fruits of thistle spotted, methionine, sodium selenite and vitamins A, E, D3. Significant violation of oxidative-antioxidant balance in animals under oxidative stress conditions, which is characterized, primarily, by activation of processes of radical lipid oxidation with excessive accumulation of both intermediate and final products of lipid peroxidation oxidation and inhibition of antioxidant defense system activity, is established. The development of oxidative stress leads to the inhibition of the activity of the glutathione system of antioxidant protection of the body of rats. This is evidenced by the low activity of glutathione peroxidase and the low level of reduced glutathione in the blood of experimental rats. The feed supplement “Butaselmeib-Plus” contributed to the activation of the system of antioxidant protection of the body of rats for tetrachloromethane poisoning, as evidenced by the increased activity of glutathione peroxidase and the level of reduced glutathione. In addition, in the blood of experimental rats, inhibition of lipid peroxidation and formation of free radicals was observed. It was determined that the level of lipids hydroperoxides on the 20th day of the experiment in blood of rats of experimental group R2 decreased by 35.7%, and the level of TBK-active products – by 21.6% relative to the indices of the first experimental group of rats. Thus, the feed supplement “Butaselmevit-Plus” when fed to rats for the development of oxidative stress inhibited lipid peroxidation, as indicated by the low level of lipids hydroperoxides and TBK-active products in their blood. This may be due to the fact that the feed additive includes two strong antioxidants, such as vitamin E and selenium, which in turn enhance the action of each other. It should also be noted that the antioxidant properties of thistle blisters, which, according to the literature, also possess antioxidant properties. It consists of vitamins B, A, E, K, precursors of vitamin D, carotenoids, macroelements – potassium, calcium, magnesium, ferrum and microelements – Cuprum, Zinc, Manganese, Iodine. The combined action of these biologically important elements exhibits high hepatoprotective and antioxidant activity.


2020 ◽  
Vol 22 (97) ◽  
pp. 52-57
Author(s):  
S. О. Slobodian ◽  
B. V. Gutyj ◽  
S. D. Murska

Heavy metals, Lead and Cadmium, are hazardous environmental pollutants. In the body of mammals, Cadmium and Lead have a toxic effect on a number of organs and systems. The mechanism of action of these poisons is to inhibit the activity of enzyme systems by blocking carboxyl, amine and SH groups of protein molecules. The aim of the study was to investigate the effect of sodium selenite and feed additive “Metisevit plus” on the morphological parameters of rat blood by Cadmium and Lead intoxication. The experiments were carried out on 200–220 g male Wistar rats, out of which 3 groups of animals were formed: a control and two experimental animals. Rats in the control and experimental groups were administered 16.6 % aqueous lead acetate at a dose of 100 mg/kg and 0.029 % aqueous cadmium chloride at a dose of 2.0 mg/kg. The rats of the first experimental group additionally used sodium selenite. The rats of the second experimental group additionally used the feed additive “Metisevit plus”. Lead-cadmium loading in rats was accompanied by erythrocytopenia and leukopenia. The lowest erythrocyte count was in the blood of diseased rats at 21 days, where it decreased by 41.3 %, respectively, compared to the initial values. It was found that hemoglobin level decreased to 90.3 ± 2.63 g/l during Cadmium and Lead intoxication in control rats. At the same time as the decrease in hemoglobin level and the number of erythrocytes in intoxicated rats, an increase in the average hemoglobin content in one erythrocyte of blood was observed up to 20.57 ± 1.20 pg As a result of the development of lead-cadmium intoxication in ill rats, leukopenia was noted, which, in our opinion, is associated with systemic lesions of cadmium and lead in the blood-forming organs of animals that suppress leukopoiesis. It was found that the number of leukocytes in patients of rats of group C decreased by 36.1 % compared with the initial data. The use of sodium selenite and the feed additive “Metisevit plus” in rats under lead-cadmium loading contributed to the increase in the number of red blood cells and hemoglobin level, as well as the decrease in the number of leukocytes in their blood. The improvement of normalizing effect on the morphological parameters of blood of rats under conditions of loading with heavy metals was shown by the feed additive “Metisevit plus” in comparison with sodium selenite.


2020 ◽  
pp. 15-18
Author(s):  
Inna R. Kilmetova ◽  
◽  
Igor A. Rodin ◽  
Nazira I. Khayrullina ◽  
Nikolay G. Fenchenko ◽  
...  

Summary. The disbalanced feeding and the uneven distribution of micro- and macroelements in the environment leads to a trace element, in particular hypomelanosis. To accelerate the growth and preservation of young farm animals include in the diet of various biological additives and drugs, which include selenium. For stimulation of weight gain in the livestock industry, as well as for the prevention and treatment of pathological processes in addition to micro - and macrouse amino acids, primarily methionine. The aim of this work was to study the influence of composition of DAFS-25+Polizon on morpho-biochemical parameters of blood and functional state of the liver in fattening bulls of black-motley breed in the conditions of the Republic of Bashkortostan. Experiments using were conducted on bull-calves of black-motley breed of the properties in the properties age from 6 to 15 months. The first experimental group during the experiment was additionally given the composition of DAFS-25+Polizon at a dose of 2 mg/kg, the animals of the control group received a standard diet. To assess the impact of the composition DAFS-25+Polizon on metabolism cattle studied morphological and biochemical indicators of blood and conducted histological examination of the liver. It is established that the use of the composition of DAFS-25+Polizon at a dose of 2 mg/kg increases the number of erythrocytes and hemoglobin in the experimental group and reduces the amount of white blood cells. The serum content of total protein, phosphorus and calcium increases in the group of experimental animals. Microscopic examination of the liver revealed no changes in the structure of the organ and hepatocytes in the experimental group, whereas in the control group hemodynamic disorders and dystrophic changes in liver cells were observed. Thus, the use of the composition DAFS-25+Polizon at a dose of 2 mg/kg of live weight in fattening bulls black-and-white breed contributes to the increase of redox processes in the body, stimulation of metabolism, prevent the development of liver disorders of cellular mechanisms of metabolism, optimizes the structure of the liver, which generally provides higher productivity.


Author(s):  
D. S. Dementiev ◽  
M. L. Kalayda ◽  
A. V. Zhigin

Currently, there is a rapid development of commercial salmon farming in Russia, which has grown almost 3 times in terms of production since 2013 and exceeded 116 thousand tons in 2020. In turn, this requires the appropriate development of the feed base and the development of methods for the effective use of fairly expensive fi sh feed. From these positions the involvement of the feed additive “Vinivet”, which is a waste of beekeeping in the feed production for fi sh as a protein additive is relevant. The feed additive “Vinivet” contains bee products such as bee bread and slumgum, a significant complex of vitamins, amino acids, macro- and microelements. The purpose of the work was to assess the influence of the feed additive “Vinivet” on the livability of juveniles, growth rate and feed consumption of rainbow trout of Steelhead salmon breed when growing in a recirculating aquaculture system. For the experiment juveniles with an average juvenile weight of 2,37±0,72 g have been selected, from which the control and 3 experimental groups have been formed. The control group has received a standard starting compound feed as the main diet, the 1st experimental group in addition to the main diet has received the additive “Vinivet” in the amount of 2,5 % of the mass of the main diet, the 2nd experimental group has received 5,0 % of the additive, and the 3rd experimental group in the first week received 2,5 % of the additive, and from the second week of studies the dose of the additive was increased to 5 %. The conducted studies have shown that the inclusion of the feed additive “Vinivet” in the feed when growing juveniles of Steelhead salmon is most advisable by introducing it at the dosage of 2,5 % of the mass of the main diet in the first week of cultivation, followed by the increase in the dose to 5,0 %, which allows achieving the highest fi sh productivity of pools in the recirculating aquaculture system compared to all variants of the experiment and by 24 % compared to the control group.


Author(s):  
L. A. Nikanova ◽  

The influence of feed additive "Ormik" consisting of organic acids (formic acid, lactic acid, orthophosphoric acid, acetic acid), as a filler of diatomite crumb, introduced into concentrated fodders, on the biochemical parameters of blood serum of pigs and on productivity is considered in the article. The studied feed additive was added to the feed daily in the form of loose powder, mixing thoroughly. The initial dosage of the feed additive in the 1st week was 1 g/kg of feed, 2nd week and the following – 3 g/kg of feed. The average daily gain in the live weight of pigs in the experimental group was 11,0% higher than the control group. The animals of the experimental group were less sick and the safety of this group was 100%, in the control group it was 90%.


Author(s):  
I. A. Pushkaryev ◽  
N. M. Kostomakhin ◽  
S. V. Burtseva ◽  
N. A. Novikov

The reproductive traits of sows are of paramount importance in the production of high-quality pork. The world experience of pig farming shows the need to solve first of all the feed problem. Diets unbalanced in the content of vitamins restrain the manifestation of genetically inherent high prolificacy of sows. The purpose of the research was to study the influence of feeding the vitamin feed additive “LipoCar” on the reproductive traits of sows and the biochemical parameters of blood serum. The results of research on the introduction of vitamin feed additive “LipoCar” into the diet of pregnant sows have been presented in the article. The experiment has been carried out in the production conditions in JSC “Linevsky pedigree farm” on sows of Large White breed. Pregnant sows have received a basic diet, balanced for all the normalized elements of nutrition. Sows of the experimental group in the composition of the basic diet fed the feed additive “LipoCar”. The dosage of the drug “Lipocar” was 2,1 g/head/day. “Lipocar” has been fed for 20 days. The total duration of the experiment was 3 months. When introducing the vitamin feed additive “LipoCar” into the main diet of sows in the second half of pregnancy the increase in the number of piglets in the litter by 1,6-6,8 % and the weight of the litter – by 5,6-56,2 % (P ≤ 0,001) have been revealed in comparison with the control group of animals. There was a higher content of carotene in the blood serum of sows of the experimental group by 50,0 % (P ≤ 0,01), vitamin A - by 48,7 % (P ≤ 0,05), and total protein – by 4,9 % (P ≤ 0,05).


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