Efficacy of extracts from cryopreserved placenta on third-degree burns in rats

Human placenta extracts have anti-inflammatory, antioxidant and wound-healing properties, so they are promising drugs for the treatment of wounds of various origins, including burns. Cryopreservation methods are widely used to preserve the biological activity of placental drugs for a long time. The aim of this work was to study the effect of low-temperature storage of the placenta on the regenerative properties of its extracts. Fragments of freshly obtained placentas were cooled by immersion in liquid nitrogen and stored at –196 °C for 6 months. The placenta was warmed in a water bath at 37 °C. The effect of low-temperature preservation of the placenta on the ability of its extracts to positively affect the wound healing process was studied in a model of thermal burn of III B degree in rats. The effectiveness of wound treatment with extracts from cryopreserved placenta was evaluated by planimetric and histological methods at 3, 7, 14, 21 and 28 days after the burn. The activity of antioxidant enzymes in the serum of animals was also determined. superoxide dismutase activity was assessed by inhibition of adrenaline autooxidation in carbonate buffer, catalase activity was assessed by the degree of inhibition of ammonium peroxide formation. It has been shown that the treatment of burns with extracts from cryopreserved placenta helped to accelerate the regeneration processes and the rate of wound healing. The formation of granulation tissue was detected on the 7th day of treatment with extracts, and on the 14th day in the control. The area of burn wounds during treatment with extracts probably differed from the control starting from 14 days after application of the burn. It was found that the dynamics of recovery of catalase activity after burns is probably higher on the 7th day of treatment with extracts. The obtained data testify to the high efficiency of application of placenta stored at low-temperature for the purpose of obtaining extracts from it with preservation of regenerative properties.

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Polymer-Based Scaffolds Loaded with Aloe vera Extract for the Treatment of Wounds

Pharmaceutics ◽

10.3390/pharmaceutics13070961 ◽

2021 ◽

Vol 13 (7) ◽

pp. 961

Author(s):

Sibusiso Alven ◽

Vuyolwethu Khwaza ◽

Opeoluwa O. Oyedeji ◽

Blessing A. Aderibigbe

Keyword(s):

Wound Healing ◽

Chronic Wounds ◽

Mechanical Performance ◽

Healing Process ◽

Aloe Vera ◽

Antimicrobial Properties ◽

Biological Properties ◽

Wound Management ◽

Wound Healing Process ◽

Treatment Of Wounds

The treatment of wounds is one challenging biomedical field due to delayed wound healing common in chronic wounds. Several factors delay wound healing, including microbial infections, malnutrition, underlying physiological conditions, etc. Most of the currently used wound dressing materials suffer from poor antimicrobial properties, poor biodegradability and biocompatibility, and weak mechanical performance. Plant extracts, such as Aloe vera, have attracted significant attention in wound management because of their interesting biological properties. Aloe vera is composed of essential constituents beneficial for the wound healing process, such as amino acids, vitamins C and E, and zinc. Aloe vera influences numerous factors that are involved in wound healing and stimulates accelerated healing. This review reports the therapeutic outcomes of aloe vera extract-loaded polymer-based scaffolds in wound management.

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Pulse Voltage Electrical Stimulation for Bacterial Inactivation and Wound Healing in Mice with Diabetes

Avicenna Journal of Medical Biotechnology ◽

10.18502/ajmb.v14i1.8175 ◽

2022 ◽

Author(s):

Mokhamad Tirono ◽

Farid Samsu Hananto ◽

Ahmad Abtokhi

Keyword(s):

Wound Healing ◽

Electrical Stimulation ◽

Treatment Time ◽

Healing Process ◽

Anaerobic Bacteria ◽

Pulse Voltage ◽

Wound Healing Process ◽

Bacterial Inactivation ◽

Wound Area ◽

Treatment Of Wounds

Background: Treatment of wounds in diabetes often gets less than perfect healing. One of the reasons for the difficulty in treating wounds in diabetes is the growth of aerobic and anaerobic bacteria. This study aims to determine the pulse voltage and treatment time that can optimally inactivate bacteria, and their effect on wound healing in mice suffering from diabetes. Methods: The study used electrical stimulation with a direct voltage of 10 volts given a pulse voltage of 50-80 volts, a width of 50 µs, and the number of pulses of 65 per second. The research samples were Staphylococcus aureus (S. aureus) and Pseudo-monas aeruginosa (P. aeruginosa) bacteria that grew on beef and mice (Mus musculus) with diabetes. The treatment for S. aureus and P. aeruginosa bacteria was carried out using a pulse voltage of 50-80 volts for 5-15 min/day and repeated for 3 days. Meanwhile, treatment of mice wounds was carried out with a pulse voltage of 80 volts for 15 min/day and repeated for 7 days. Results: The results showed that treatment with a pulse voltage of 50-80 volts and a treatment time of 5-15 min significantly reduced the number of S. aureus and P. aeruginosa bacteria in beef (p£0.05). Treatment with a pulse voltage of 80 volts for 15 min made beef free from bacteria. Meanwhile, treatment with a pulse voltage of 80 volts for 15 min per day for seven days resulted in the wound state of three mice in the maturation phase and two mice in the proliferation phase on day 8 with an average wound area of 0.108 cm 2. Conclusion: The treatment with a pulse voltage of 80 volts for 15 min made the beef sterile, the mice wounds healed quickly, and the mice not stressed. The higher the blood glucose level, the slower the wound healing process.

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Wound-healing activity of immobilized postbiotics from Bacillus subtilis exometabolites

BIO Web of Conferences ◽

10.1051/bioconf/20214001002 ◽

2021 ◽

Vol 40 ◽

pp. 01002

Author(s):

Dina Shokatayeva ◽

Irina Savitskaya ◽

Aida Kistaubayeva

Keyword(s):

Wound Healing ◽

Bacillus Subtilis ◽

Healing Process ◽

Antagonistic Activity ◽

Healing Time ◽

Wound Dressings ◽

Laboratory Animals ◽

Wound Healing Process ◽

Antimicrobial Substances ◽

Treatment Of Wounds

The biological activity of postbiotic from Bacillus subtilis exometabolites was determined due to protein content - 0.541±13.4 mg/ml, the level of proteases - 7.8±0.3 U/ml and the presence of antimicrobial substances. A biocomposite material was developed by co-aggregation of bacterial cellulose, chitosan and Bacillus exometabolites. Modified BC gel film possesses high antagonistic activity against causative agents of wound infections: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis. The use of the obtained material in treatment of wounds on laboratory animals reduces healing time by an average of 20%. The developed bioactive wound dressings is intended for local application in order to optimize the wound healing process.

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PHOTODYNAMIC THERAPY EFFECTS ON MICROFLORA IN THE TREATMENT OF PURULATORY-NECROTIC PROCESSES OF SOFT TISSUES (CLINICAL AND EXPERIMENTAL WORK)

Kharkiv Surgical School ◽

10.37699/2308-7005.2.2020.05 ◽

2020 ◽

pp. 26-29

Author(s):

Y. D. Khvorostov ◽

S. O. Morozov ◽

H. M. Herasymov ◽

V. V. Derikolenko ◽

S. S. Solodovidchenko

Keyword(s):

Wound Healing ◽

Photodynamic Therapy ◽

Experimental Work ◽

Soft Tissues ◽

Healing Process ◽

Wound Healing Process ◽

Complex Treatment ◽

Bacterial Composition ◽

Purulent Wounds ◽

Treatment Of Wounds

Summary. The effect of PDT on the wound healing process and the Bacterial composition of wounds has not been fully studied. Material and methods. The effects of PDT of various wavelengths on the most common microflora of purulent wounds and the course of the wound process are investigated. Results and discussion. The application of PDT increased the sensitivity of microflora to ABD, and also stimulated reparative processes — the growth of granulation tissue and marginal epithelization. Conclusions. The application of PDT in the complex treatment of wounds favorably affects wound healing, expanding the possibilities of AB therapy

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Antibacterial and anti-inflammatory activity of extracts and major constituents derived from Stachytarpheta indica Linn. leaves and their potential implications for wound healing

10.21203/rs.3.rs-336156/v1 ◽

2021 ◽

Author(s):

Vajira Asela Agampodi ◽

Peter Katavic ◽

Christopher Collet ◽

Trudi Collet

Keyword(s):

Wound Healing ◽

Plant Extracts ◽

Colorimetric Assay ◽

Morphological Changes ◽

Healing Process ◽

Human Keratinocytes ◽

Wound Healing Process ◽

Keratinocyte Proliferation ◽

Anti Inflammatory ◽

Treatment Of Wounds

Abstract Wounds of various types continue to have a severe socioeconomic impact on the cost of health care. Globally, there has been increased interest surrounding the identification of bioactive compounds that promote or modulate the wound healing process. Stachytarpheta indica Linn. is traditionally used to heal wounds and relieve inflammation, however, the theorised pharmacological properties have not yet been scientifically validated. In this study, dried and ground plant leaves were extracted with water and methanol, which were then subjected to various analyses. Antimicrobial activity of the plant extracts and isolated compounds was determined using well diffusion assays, while the minimum inhibitory concentrations were determined with a colorimetric assay. Morphological changes of human keratinocytes in response to plant extracts were observed with differential interference contrast microscope imaging. Cell viability, proliferation and migratory effects post-treatment with the plant extracts were also evaluated via colorimetric cytotoxicity assays and a real-time cell analyser protocol. Anti-inflammatory effects of plant extracts and isolated compounds were evaluated by flow cytometry and cyclooxygenase and lipoxygenase enzyme inhibition assays. Three active compounds i.e. ipolamiide, verbascoside and iso-verbascoside, were isolated from S. indica leaves. Verbascoside demonstrated broad-range antibacterial activity and imposed strong inhibition at 9.77 μg/mL against Staphylococci spp. S. indica extracts (0.1-0.2 mg/mL) were shown to improve human keratinocyte proliferation up to 60 % and induce morphological changes by producing cytoplasmic projections at concentrations higher than 0.4 mg/mL. Plant extracts (6.25-100 μg/mL) and individual compounds (3.125-50 μg/mL) elicited strong anti-inflammatory effects by suppressing the expression of interleukin-8 and inhibiting cyclooxygenase-1 and 5-lipoxygenase enzymes. Collectively, these results indicate that plant extracts and isolated compounds derived from S. indica have the potential to inhibit bacterial growth, promote tissue regeneration and reduce inflammation, hence, potentially providing the basis for a novel therapeutic for the treatment of wounds.

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SURVEY OF ETHNO MEDICINAL PLANT SHOWING WOUND HEALING ACTIVITY

International Journal of Advanced Research ◽

10.21474/ijar01/13364 ◽

2021 ◽

Vol 9 (08) ◽

pp. 1142-1159

Author(s):

Ansumita Borsaikia ◽

◽

Saikat Sen ◽

Pal Gogoi ◽

Biplab Kumar Dev ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Body Part ◽

Wound Management ◽

Animal Origin ◽

Wound Healing Process ◽

Surgical Interventions ◽

Normal Wound Healing ◽

Time Required ◽

Treatment Of Wounds

In most recent couple of a few years, there has been a unprecedented advancement in understanding the biochemical and cell occasions of typical injury recuperating or wound healing. Healing is survival mechanism and represents a trial to take care of normal complex body part and performance.Wound healing could be a process by which tissue regeneration occurs. Healing of wounds, either accidental or surgical interventions, involves complex activities of blood cells, tissues, soluble mediators, cytokines and several other growth factors. This increased cellular activity of damaged tissue enhances metabolic demands and active drug therapy. The main objective of treating a wound is to either shorten the time required for healing process or to reduce the undue effects. Plants because of presence of assorted valuable active phytoconstituents have immense potential for management and treatment of wounds over the years. All the Traditional systems of medicine, Ayurveda, Siddha and Unani describe applications of medication of plant, mineral and animal origin to treat and heal wounds. Herbal drugs induce healing and regeneration of lost tissue by number of mechanisms. Due to their traditional applicability, affordability and safety plants gained a reputed position within the world of wound management and repair.The present review is a trial to focus on various Indian ethno-medicinal plants which are to be scientifically proved for the treatment of wounds. Beside this review also emphasis on normal wound healing process, pharmacological activities and role of plants in wound management and parameters accustomed assess wound healing.

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Canine-Origin Platelet-Rich Fibrin as an Effective Biomaterial for Wound Healing in Domestic Cats: A Preliminary Study

Veterinary Sciences ◽

10.3390/vetsci8100213 ◽

2021 ◽

Vol 8 (10) ◽

pp. 213

Author(s):

Carla S. Soares ◽

Isabel R. Dias ◽

Maria A. Pires ◽

Pedro P. Carvalho

Keyword(s):

Wound Healing ◽

Healing Process ◽

Cost Effective ◽

Wound Healing Process ◽

Platelet Rich Fibrin ◽

Cost Effective Treatment ◽

Antiseptic Treatment ◽

High Concentrations ◽

Biological Cost ◽

Treatment Of Wounds

Platelet-rich fibrin (PRF) is a recent platelet-based biomaterial, poised as an innovative regenerative strategy for the treatment of wounds from different etiologies. PRF is defined as a biodegradable scaffold containing elevated amounts of platelets and leukocytes having the capability to release high concentrations of bioactive structural proteins and acting as a temporal release healing hemoderivative. This study aimed to evaluate the performance of canine-origin PRF, obtained from blood of screened donors, as a regenerative biomaterial suitable for the treatment of critical wounds in felines. Four short-hair felines with naturally occurring wounds were enrolled in this study. Three of the wounds were considered infected. Each PRF treatment was the result of the grafting of newly produced PRFs at the recipient area. The PRF treatment was initially performed two to three times per week, followed by single weekly treatments. The study was finalized when complete wound closure was achieved. No topical antimicrobial/antiseptic treatment was applied. The present research demonstrated that xenogenic PRFs significantly induced healthy vascularized granulation tissue in lesions with soft tissue deficit, also prompting the epithelization at the injured site. No rejection, necrosis, or infection signs were recorded. Additionally, PRF-therapy was revealed to be a biological cost-effective treatment, accelerating the wound healing process.

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A Review on Polymeric Wound Dress for the Treatment of Burns and Diabetic Wounds

International journal of basic science in medicine ◽

10.34172/ijbsm.2021.08 ◽

2021 ◽

Vol 6 (2) ◽

pp. 44-50

Author(s):

Ashkan Farazin ◽

Zahra Torkpour ◽

Shakiba Dehghani ◽

Ramin Mohammadi ◽

Mina D. Fahmy ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Polymeric Materials ◽

Skin Surface ◽

Wound Management ◽

Wound Healing Process ◽

Diabetic Patients ◽

Natural Polymers ◽

Treatment Of Wounds ◽

The Right

Today, various commercial dressings have been developed and introduced to the market. The diversity makes it difficult for the nurse to choose the right type. Although the most important reason to use a wound dress is to protect the wound from infection and prevent infection, but in fact, the main purpose of using these materials is to speed up the wound healing process. Traumatic injuries result in an epithelial wound that disrupts the continuity of the skin surface. These differences reveal as abrasions, punctures, and injuries. Wounds are divided into two types; the skin is either cut or ruptured, including deep wounds and bruises, or surface wounds. Expedited wound healing has been considered since the archaic era of human civilization, with the earliest reported case from the Ancient Egyptians. Wound lesions in mummified humans were observed to be cover with animal skin, with signs of (primary/secondary) wound healing present. A "new wound dressing" is an advanced wound dress used in wound management as biocompatible and biodegradable biomaterials that heal wounds and burns. In the past, it was believed that dry wounds had expedited healing and wet wounds have been found to promote using re-epithelization and result in reduced scar formation. Wounds can be treated using various types of natural polymers and materials. Also, techniques like electrospinning and freeze-drying techniques can be used for the fabrication of standard wound dress. These wounds are associated with bandages, inflammation, bleeding, pain, and pus. In this work, we consider various types of wounds and techniques to treat the wound. Susceptibility to these areas, due to special symptoms for each of them. Products like hydrogels, hydrocolloids, films, sponges, and nano-fiber polymeric materials are used to promote healing. In this review, we examine the ideal products for the treatment of wounds in diabetic patients.

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Ultrasonic cavitation in the treatment of neuro-ischemic diabetic foot the presence of biofilm forms of bacteria (literature review)

Wounds and wound infections The prof B M Kostyuchenok journal ◽

10.25199/2408-9613-2020-7-3-20-31 ◽

2021 ◽

Vol 7 (3) ◽

pp. 20-30

Author(s):

V. A. Mitish ◽

Yu. S. Paskhalova ◽

P. A. Munioz Sepeda ◽

A. A. Ushakov ◽

L. A. Blatun ◽

...

Keyword(s):

Diabetes Mellitus ◽

Wound Healing ◽

Diabetic Foot ◽

Healing Process ◽

Additional Treatment ◽

Ultrasonic Cavitation ◽

Wound Healing Process ◽

Treatment Protocols ◽

Number Of Patients ◽

Treatment Of Wounds

The constant increase in the number of patients with diabetes mellitus, among whom the incidence of long-term non-healing wounds is many times higher than in the general population, requires constant analysis of treatment protocols and their correction, if necessary. The peculiarities of the wound healing process in the presence of diabetes mellitus include phase perversion and reduced reparative potential. Another problem is the infectious process taking place against the background of diabetes mellitus. Its features include a tendency towards chronicity, frequent persistence of resistant and multiresistive forms of bacteria, and the formation of biofilms. All these factors are pushing to search for new approaches to treatment, and one of the dynamically developing areas is additional treatment of wounds with various types of physical energies. On the one hand, the use of negative pressure, ultrasound, plasma flows, a pulsating jet of liquid, etc. of the wound healing process has been studied for several decades. At the same time, there are still many unanswered problems. One of them is the effectiveness of ultrasonic cavitation in the treatment of purulent-necrotic complications of the neuro-ischemic diabetic foot in the presence of biofilm forms of bacteria in the wound.

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Effects of Alchemilla mollis and Alchemilla persica on the wound healing process

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v11i3.26024 ◽

2016 ◽

Vol 11 (3) ◽

pp. 577

Author(s):

Burçin Ergene Öz ◽

Mert Ilhan ◽

Serkan Ozbilgin ◽

Esra Küpeli Akkol ◽

Özlem Bahadır Acıkara ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Video Clip ◽

Wound Healing Process ◽

Histopathological Analysis ◽

Excision Wound ◽

Wound Model ◽

Aerial Parts ◽

Anti Inflammatory ◽

Treatment Of Wounds

Alchemilla mollis, is used in traditional medicine for the treatment of wounds and excessive menstruation. Aqueous methanol extracts of A. mollis and A. persica were evaluated for wound healing acivity by using linear incision and circular excision wound models along with hydroxyproline estimation and histopathological analysis. Anti-inflammatory effect was determined according to Whittle method. The extracts prepared from the aerial parts of A. mollis and A. persica exerted significant wound healing activity with the tensile strength values of 39.3% and 33.3%, respectively, and with the contraction values of 51.4% and 43.5%, respectively. Hydroxyproline estimation and histopathological analysis also confirmed the results. The extracts of A. mollis and A. persica showed significant anti-inflammatory activity with the values of 30.6% and 26.6% respectively. These results showed that A. mollis and A. persica possess significant wound healing and anti-inflammatory activities.Video Clip<a href="https://youtube.com/v/k2Hz7f1e4SI">Circular excision wound model</a>: 2 min

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