scholarly journals Effects of subclinical Mycobacterium avium ssp. paratuberculosis infection on some physiological parameters, health status and production in dairy cows

2016 ◽  
Vol 64 (3) ◽  
pp. 301-312 ◽  
Author(s):  
Viktor Jurkovich ◽  
Barbara Bognár ◽  
Krisztián Balogh ◽  
Mária Kovács-Weber ◽  
Kinga Fornyos ◽  
...  

Milk yield, milk ingredients, health and other, production-related parameters of subclinically infected, Mycobacterium avium ssp. paratuberculosis (MAP-) shedding (positive faecal PCR, n = 20) and non-shedding (negative faecal PCR, n = 10) dairy cows were compared in the period from 10 days prepartum to 120 days postpartum. Body condition, rumen fill and faeces scores were lower in the MAP-shedding cows. There was no significant difference in plasma or urine metabolic parameters between the groups. Milk yield and lactose content tended to be lower (P = 0.074 and 0.077, respectively), somatic cell count tended to be higher (P = 0.097), while milk fat content was significantly higher (P = 0.006) in MAP-shedding cows than in the controls. Milk protein content did not differ between the groups. All other health and production parameters [number of reproductive tract treatments, number of udder treatments, number of artificial inseminations (AIs), calving interval, and service period] were significantly better in the control group. It is concluded that MAP infection, even in a subclinical form, has a significant impact on some production and health parameters of dairy cows.

Author(s):  
A Gavelis ◽  
V. Þilaitis ◽  
A Juozaitis ◽  
V. Juozaitienë ◽  
G. Urbonavièius ◽  
...  

The aim of this study was to evaluate relationship between milk progesterone concentration (P4) and milk traits at the start of estrus time and 12h after start of the estrus in dairy cows. The 96 milk samples of 48 Lithuanian dairy cows without reproduction disorders and 90–100 days after calving were evaluated. Cows were classified into two groups based on milk yield per day: less than 30 kg (n=20) and e”30 kg (n=28). Data were categorized by milk fat and protein content at the start estrus and 12h after start of estrus to evaluate relationship between P4 and milk traits examined. P4 at estrus time in dairy cows was significantly positively correlated with milk yield (P less than 0.001), whereas it was negatively correlated with milk protein (P less than 0.05-P less than 0.01) and fat at 12h after start of estrus. Dairy cows with F/P from 1.0 to 1.5 had the lowest P4 in milk. Results of the pregnancy in dairy cows were related with lower P4 and milk yield level (P less than 0.001), higher milk fat (P less than 0.05) and milk protein content (P less than 0.001). These cows had 1.90 times lower prevalence of the signs of subclinical ketosis (P less than 0.05) at estrus time when compared with non-pregnant cows. As a result, it was clearly demonstrated that P4 in dairy cows can help to evaluate and improve the reproductive properties of cows.


2005 ◽  
Vol 45 (4) ◽  
pp. 331 ◽  
Author(s):  
W. J. Fulkerson ◽  
K. McKean ◽  
K. S. Nandra ◽  
I. M. Barchia

Two experiments were conducted, each over several months, when cows grazed either ryegrass (September–November 2001) or kikuyu (February–March 2002) pastures, to assess the effects of accurately allocating feed on a daily basis to lactating Holstein–Friesian dairy cows. In each case, 28 cows were randomly stratified into 2 equal groups on the basis of milk and milk component yield, liveweight, age and days in lactation. The metabolisable energy requirements of the animals were estimated from standard established requirements. In each experiment, both groups of cows received the same amount of supplement over a period that was equivalent to a pasture regrowth cycle of 12–16 days. The control group received a set amount of supplements each day, while supplements fed to the adjusted group varied, dependent on pasture available. Available pasture was varied from 7 to 21 kg DM/cow.day (above a stubble height of 5 cm), to mimic the variation found on well-managed dairy farms. When pasture available was above the predicted requirement for cows in the adjusted group, pasture availability was restricted to predicted requirements and the extra milk that could be produced from the spared pasture was estimated. However, cows in the control group had the opportunity to eat more pasture if allocated more than required. This could result in more milk being produced, a gain in liveweight, and/or a higher post-grazing pasture residue (and hence potentially improve pasture regrowth). If less pasture than required was allocated to the control group, production could reduce or the cows might graze harder. Thus, in the control group the proportion of forage to supplement remained relatively constant, but intake varied in relation to pasture allocated, while for the adjusted group the total intake was kept relatively constant. In experiment 1 (ryegrass), the milk yield, percentage of milk fat and liveweight change of cows in the control and adjusted groups was not significantly different. However, the cows in the adjusted group produced 0.016 kg/cow.day more milk protein. As the control group ate 0.35 kg DM/cow.day more ryegrass pasture (P = 0.008) it is assumed that accurate daily allocation of feed improved feed efficiency. In experiment 2, the milk yield and percentage of milk protein of cows grazing kikuyu pastures was not significantly different between groups but the percentage of milk fat and covariate-corrected liveweight at the end of the experiment was higher in the control group than in the adjusted group. The pasture spared by cows in the adjusted group was predicted to produce 8.9% more milk when grazing ryegrass pasture and 12.3% when grazing kikuyu pasture. Linear regression analysis of pasture on offer on post-grazing pasture residue was not significant for the cows in the adjusted group but was significant for the control group cows when grazing either pasture, indicating success in accurately allocating supplementary feed to maintain a constant grazing pressure. The results of this study should assist dairy farmers in deciding whether the effort required to allocate feed accurately to dairy cows on a daily basis, is worthwhile.


1999 ◽  
Vol 66 (4) ◽  
pp. 475-487 ◽  
Author(s):  
ZYGMUNT M. KOWALSKI ◽  
PAWEŁ M. PISULEWSKI ◽  
MAURO SPANGHERO

The objective of this study was to determine the effects of supplementing the diets of dairy cows with Ca soaps of rapeseed fatty acids (CSRFA) and rumen-protected (RP) methionine on their milk yield and composition, including milk protein fractions and fatty acids. Twelve Polish Red Lowland cows were used in a complete balanced two period changeover experiment. The four treatment diets were a control consisting of a total mixed ration of grass silage and concentrates, and the total mixed ration supplemented with RP methionine, CSRFA or RP methionine plus CSRFA. Dry matter intake was not affected by diet. Milk yield increased when cows were given the diet with CSRFA, but supplementation of diets with RP methionine did not affect milk yield. Milk protein content, but not milk protein yield, decreased when CSRFA was given. The addition of RP methionine to the control diet and the CSRFA diet produced similar increases in the milk protein content. Supplementation of the diet with CSRFA significantly changed the milk fatty acid profile: the proportions of 10:0, 12:0, 14:0, 15:0 and 16:0 in milk fat decreased, but those of 18:0 and cis-18:1 increased. We conclude that CSRFA can be used in practical dairy diets to increase milk yield and manipulate its fatty acid composition.


1996 ◽  
Vol 62 (1) ◽  
pp. 1-3 ◽  
Author(s):  
P. C. Garnsworthy

AbstractTwenty-eight Holstein/Friesian dairy cows were divided into four groups of seven. From weeks 4 to 15 of lactation they were given a basal diet consisting of 8 kg hay, 2 kg sugar-beet feed and 2 kg grass nuts, together with a concentrate allowance of 8 kg/day. Concentrates for group A were based on cereals and soya (control). Concentrate B contained 60 g protected fat supplement per kg; concentrate C contained 100 g lactose per kg; concentrate D contained 60 g fat supplement and 100 g lactose per kg. Milk yields were 24·6, 27·7, 25·6 and 26·5 kg/day and milk protein concentrations were 32·3, 30·7, 32·7 and 31·9 g/kg for groups A, B, C and D respectively. The effect of fat supplementation on milk yield and protein concentration was significant (P < 0·05) but the effect of lactose was not significant. Milk fat concentration was not significantly affected by treatment. It is concluded that lactose can partially alleviate the depression in milk protein concentration often observed when cows are given protected fat.


Author(s):  
Martin Skýpala ◽  
Gustav Chládek

Milk yield varies during lactation, following what is termed a lactation curve. ŽIŽLAVSKÝ and MIKŠÍK (1988) recorded changes in milk yield within a day, too. TEPLÝ et al. (1979) a KOUŘIMSKÁ et al. (2007) published variation within a day ± 1.10 kg in milk yield, ± 0.75 % in milk fat content and ± 0.20 % in milk protein content. Milk yield of cows can be expressed in many different ways, for instance, in kilograms per lactation or in kilograms per day. A practical parameter describing milk production is milk yield (kg) per milking.The object of experiment were 12 cows of Holstein cattle on the first lactation from the 100-day of lactation to 200-day of lactation. The samples of milk were collected from January to May 2007, once a month from the morning and evening milking (milking interval 12 h ± 15 min.). The following parameters were monitored: milk production – milk yield (kg), milk protein production (kg), milk fat production (kg); milk composition – milk protein content (%), milk fat content (%), lactose content (%), milk solids-not-fat content (%), milk total solids content (%); technological properties of milk – ti­tra­tab­le acidity (SH), active acidity (pH), rennet coagulation time (s), quality of curd (class) and somatic cell count as a parameter of udder health.Highly significant differences were found (P < 0.01) between morning milk yield (15.7 kg) and evening milk yield (13.8 kg), between morning milk protein production (0.51 kg) and evening milk protein production (0.45 kg) and between evening milk fat content (4.41 %) and morning milk fat content (3.95 %). A significant difference (P < 0.05) was found between morning milk total solids content (12.62 %) and evening milk total solids content (12.07 %). No significant differences were found between morning (M) and evening (E) values of the remaining parameters: milk fat production (M 0.62 kg; E 0.60 kg), milk protein content (M 3.24 %; E 3.27 %), milk lactose content (M 4.78 %; E 4.86 %), milk solids-not-fat content (M 7.69 %; E 7.71 %), somatic cell count (M 80 000/1 mL; E 101 000/1 mL), titratable aci­di­ty (M 7.75 SH; E 7.64 SH), active acidity (M pH 6.58; E pH 6.61), rennet coagulation time (M 189 s.; E 191 s.), quality of curd (M 1.60 class; E 1.57 class).


Author(s):  
D. Tristant ◽  
C. A. Moran

SummaryThe following trial was conducted to evaluate the impact of feeding Yea-Sacc® (YS; Alltech Inc, USA), a zootechnical feed additive based on a live probiotic strain of Saccharomyces cerevisiae, to lactating dairy cows over a 12 week period. Sixty-four primiparous and multiparous Holstein dairy cows, grouped to give similar range of parity, physiological and milk production stages, were selected for the study. Cows were equally allocated to either a control feed group or a diet supplemented with YS (32 cows per treatment). The test diet was formulated to include YS (Yea-Sacc® Farm Pak) incorporated in the total mixed ration (TMR), supplying a target dose of 5 × 107 CFU/kg feed dry matter (DM). This target dose delivered 1 × 109 CFU/cow/day, for a cow consuming 20 kg feed (DM basis) daily. Each cow was considered a replicate unit. Cows were fed a nutritionally adequate total TMR plus hay and a supplementary protein/energy concentrate (calculated according to milk yield) for 12 weeks, supplied once a day after the morning milking. Weigh backs of feed were recorded daily, with refusals being maintained at 3% of the total intake. During the 12 week study period, YS had significant beneficial effects on milk production (+0.8 kg/day; P = 0.003), energy corrected milk production (+1.4 kg/day; P < 0.0001), synthesis of milk protein (+36 g/day; P = 0.001), milk protein content (+0.3 g/kg; P = 0.009), and milk urea content (−0.09 mg/l; P = 0.004). The synthesis of milk fat was similar between treatments but milk fat content was lower for the YS group compared to the control group (−1.1 g/kg; P = 0.0002). Lactose content was always higher (+0.8 g/kg; P < 0.0001) for the YS group, indicating enhanced energy utilisation. In general, the effect of YS was higher during the first study period (one to seven weeks), when cows were in early lactation and the production potential was higher. YS cows produced significantly more milk during the study, and an additional 220 kg milk per cow was sold from this group from the output measured from the beginning of the study to two weeks post-trial. However, the statistical analysis including the post-study period did not show a significant effect. The 305-day simulated milk production was higher for the YS group (+400 kg/cow) but again the difference was not significant. In conclusion, YS at a target dose of 5 × 107 CFU/kg DM improved milk production and milk quality in healthy dairy cows. In addition, when the data were included in a whole-farm model, feeding YS reduced methane emissions by 4%, reduced the number of animals required for the desired milk production by 4% and increased overall farm margins by 1.4%.


Author(s):  
Marinela ENCULESCU

The aim of this study was to evaluate the effects of fresh yeast (Saccharomyces cerevisiae) supplementation in the dairy cows’ diets on productive performances and health status. The study was carried out in the experimental farm of the Research and Development Institute for Bovine Balotești on 50 multiparous Romanian Black and Spotted dairy cows, randomly divided into two groups (N = 25 heads/group), according to age, milk yield, body weight and health status. The experimental group received 80 g Saccharomyces cerevisiae/head/day for one year. The groups were fed with the same diet and had free access to water and salt. Results were expressed as a mean (±Standard Deviation). The t-test was applied to obtain the significance of difference. Supplementation of the diet with Saccharomyces cerevisiae had a significant effect (P <0.001) on milk yield (20.71±1.65 l/head/day) for the experimental group comparing with the control group (18.22±1.81 l/head/day), and on milk protein and lactose (P <0.05). The addition of Saccharomyces cerevisiae in dairy cows’ diet did not improve the milk fat, hematological and biochemical/urine indicators in the experimental group (P >0.05). However, for alkaline phosphatase, differences at the end of the study have been observed (P <0.01). The beneficial effect of the yeast and yeast products in ruminants could be attributed to microbial activity by increasing the number of beneficial bacteria in the rumen of the animals. The use of Saccharomyces cerevisiae as an alternative source of economic protein, vitamins and minerals in dairy cows’ diet represents an effective measure to optimize animal productivity.


Animals ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 57 ◽  
Author(s):  
Frank Dunshea ◽  
Kehinde Oluboyede ◽  
Kristy DiGiacomo ◽  
Brian Leury ◽  
Jeremy Cottrell

Betaine is an organic osmolyte sourced from sugar beet that accumulates in plant cells undergoing osmotic stress. Since the accumulation of betaine lowers the energy requirements of animals and, therefore, metabolic heat production, the aim of this experiment was to investigate if betaine supplementation improved milk yield in grazing dairy cows in summer. One hundred and eighteen Friesian × Holstein cows were paired on days in milk and, within each pair, randomly allocated to a containing treatment of either 0 or 2 g/kg natural betaine in their concentrate ration for approximately 3 weeks during February/March 2015 (summer in Australia). The mean maximum February temperature was 30 °C. Cows were allocated approximately 14 kg dry matter pasture and 7.5 kg of concentrate pellets (fed in the milking shed) per cow per day and were milked through an automatic milking system three times per day. Betaine supplementation increased average daily milk yield by over 6% (22.0 vs. 23.4 kg/day, p < 0.001) with the response increasing as the study progressed as indicated by the interaction (p < 0.001) between betaine and day. Milk fat % (p = 0.87), milk protein % (p = 0.90), and milk somatic cell count (p = 0.81) were unchanged by dietary betaine. However, betaine supplementation increased milk protein yield (677 vs. 719 g/day, p < 0.001) and fat yield (874 vs. 922 g/day, p < 0.001) with responses again being more pronounced as the study progressed. In conclusion, dietary betaine supplementation increased milk and component yield during summer in grazing dairy cows.


1990 ◽  
Vol 57 (3) ◽  
pp. 285-294 ◽  
Author(s):  
J. Eric Hillerton ◽  
Christopher H. Knight ◽  
Alan Turvey ◽  
Stephen D. Wheatley ◽  
Colin J. Wilde

SummaryGroups of lactating cows and heifers were milked four times daily in two diagonally opposed glands for 4 weeks, and the effects on milk yield studied relative to twice-daily milked glands as controls. Mammary enzyme activities, in vitro synthesis rates of milk constituents and histological scoring were determined in mammary biopsy samples obtained at the end of this period. These were used for assessment of mammary function. Frequent milking increased milk yield only in the treated glands, the contralateral control glands continuing to decline in yield at ~ 2%/week. There was no significant difference in response between cows and heifers; the mean increase in yield was 10·4%. The rate of decline in milk yield tended to decrease with frequent milking, to ~ 1%/week. Consequently the yield of the treated glands continued to be elevated above that of the controls for some time after reversion to overall twice daily milking. Milk protein content was increased slightly by frequent milking. Mammary enzyme activities were ~ 18% higher in the treated glands than in the controls. Synthesis rates of lactose, casein and total protein were unaffected by milking frequency, but were all lower in the gland selected for the second biopsy, reflecting the reduction in milk yield caused by the first biopsy. DNA synthesis was increased by milking frequency, as were the size and number of epithelial cells in histological sections.


2009 ◽  
Vol 77 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Roland G Karcagi ◽  
Tibor Gaál ◽  
Piroska Ribiczey ◽  
Gyula Huszenicza ◽  
Ferenc Husvéth

The aim of the study was to test the effect of rumen-inert fat supplements of different chemical forms or containing different unsaturated/saturated (U/S) fatty acid contents on milk production, milk composition and liver and blood metabolic variables of high-yielding dairy cows in the peripartal period. Thirty Holstein-Friesian dairy cows were divided into three equal groups and fed a corn silage-based diet, without fat supplementation (control) or supplemented with 11·75 MJ NEl per day of calcium soaps of palm oil fatty acids (CAS; U/S=61/39) or with 11·75 MJ NEl per day of hydrogenated palm oil triglyceride (HTG; U/S=6/94). Each diet was fed from 25±2 d prior to the expected calving to 100±5 d post partum. Compared with the control, both CAS and HTG supplementation resulted in an increase of the average milk yield. Milk fat content and fat-corrected milk yield were higher in the HTG group but lower in the CAS group than in the control group. In all groups liver triglyceride concentrations (TGL) increased from 15 d prepartum to 5 d post partum, and then decreased thereafter. At 5 d TGL was lower in the HTG group than control or CAS cows. No significant differences were detected in TGL among dietary treatments at 15 d prepartum and 25 d post partum. Higher plasma glucose and insulin and lower non-esterified fattay acids and β-hydroxybutyrate concentrations and aspartate aminotransferase activity were measured in the HTG group than in the control or CAS groups at 5 d or 25 d post partum. Our results show that HTG may provide a better energy supply for high-yielding dairy cows in negative energy balance than CAS around calving.


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