scholarly journals Effects of Saccharomyces cerevisiae Addition in Dairy Cows Diets

2021 ◽  
Vol 78 (1) ◽  
pp. 18
Author(s):  
Marinela ENCULESCU
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Health Status ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Control Group ◽  
Free Access ◽  
Alternative Source ◽  
Effective Measure ◽  
Experimental Group

The aim of this study was to evaluate the effects of fresh yeast (Saccharomyces cerevisiae) supplementation in the dairy cows’ diets on productive performances and health status. The study was carried out in the experimental farm of the Research and Development Institute for Bovine Balotești on 50 multiparous Romanian Black and Spotted dairy cows, randomly divided into two groups (N = 25 heads/group), according to age, milk yield, body weight and health status. The experimental group received 80 g Saccharomyces cerevisiae/head/day for one year. The groups were fed with the same diet and had free access to water and salt. Results were expressed as a mean (±Standard Deviation). The t-test was applied to obtain the significance of difference. Supplementation of the diet with Saccharomyces cerevisiae had a significant effect (P <0.001) on milk yield (20.71±1.65 l/head/day) for the experimental group comparing with the control group (18.22±1.81 l/head/day), and on milk protein and lactose (P <0.05). The addition of Saccharomyces cerevisiae in dairy cows’ diet did not improve the milk fat, hematological and biochemical/urine indicators in the experimental group (P >0.05). However, for alkaline phosphatase, differences at the end of the study have been observed (P <0.01). The beneficial effect of the yeast and yeast products in ruminants could be attributed to microbial activity by increasing the number of beneficial bacteria in the rumen of the animals. The use of Saccharomyces cerevisiae as an alternative source of economic protein, vitamins and minerals in dairy cows’ diet represents an effective measure to optimize animal productivity.

IMPACT OF NEW ACTIVATOR RUMINAL MICROFLORA ON DAIRY COWS EFFICIENCY

2020 ◽  
Author(s):  
Д.Ю. ГРИГОРЬЕВ ◽  
Д.А. ПИРОГОВ ◽  
Д.В. ФРИЗЕН
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Milk Yield ◽  
Milk Fat ◽  
Economic Effect ◽  
Control Group ◽  
Entire Group ◽  
Average Content ◽  
Daily Milk Yield ◽  
Experimental Group ◽  
Daily Milk

Изучено влияние на продуктивность коров нового активатора рубцовой микрофлоры — «МегаБуст Румен» (МБР) на основе инактивированного ферментационного экстракта Trichoderma longibrachiantum и специализированного штамма дрожжей Saccharomyces cerevisiae в двух опытах. В первом эксперименте в опытной группе коровы в транзитный период получали по 100 г/день МБР за 7 дней до отела и с 1 по 150-й день лактации, в остальном содержание и кормление животных опытной (n=10) и контрольной (n=10) групп было идентичным. В среднем в сутки коровы в опытной группе давали молока 41,26±0,91 кг при среднем содержании молочного жира 3,8±0,33% и белка 3,34±0,06%, что на 6,81±0,62 кг молока больше (Р<0,05), чем в контрольной группе (34,82±0,78 кг при среднем содержании молочного жира 3,55±0,13 и белка 3,08±0,09%). Использование МБР в опытной группе способствовало существенному увеличению среднесуточного удоя (+1500 кг) в опытной группе по сравнению с контрольной на 1 голову, пересчитанного на содержание жира 3,5%, за первые 150 дней лактации. Экономический эффект применения МБР — это дополнительный чистый доход на 1 голову за период эксперимента — 40037 руб. Во втором опыте, который проводился на всей группе лактирующих коров, при скармливании на 1 голову в сутки 100 г МБР отмечали повышение добровольного потребления сухого вещества (+2 кг СВ), увеличение среднесуточного удоя (+4,4 кг) на 1 голову в день. Возврат инвестиций в активатор рубцовой микрофлоры на 1 руб. составил 12,7 руб. The effect of new rumen microflora activator — MegaBust Rumen (MBR) on the basis of inactivated fermentation extract Trichoderma longibrachiantum and a specialized strain of yeast Saccharomyces cerevisiae on the cows productivity was studied in two experiments.In the first experiment, in the experimental group, the cows received MBR in the dose 100 g/day in the transit period 7 days before calving and from the 1st to the 150th day of lactation, otherwise the keeping and feeding of the animals of the experimental and control groups were identical. In average, per day, cows in the experimental group gave milk 41.26±0.91 kg with an average content of milk fat of 3.8±0.33% and protein of 3.34±0.06%, which is 6.81±0,62 kg of milk is more (P<0.05) than in the control group (34.82±0.78 kg with an average content of milk fat of 3.55±0.13 and protein of 3.08±0.09%). The use of MBR in the experimental group contributed to a significant increase in the average daily milk yield (+1500 kg) in the experimental group compared to the control for 1 head, calculated on the fat content of 3.5% for the period of first 150 days of lactation. The economic effect of the use of MBR is an additional net income per head for the period of the experiment was — 40037 rubles. In the second experiment, which was conducted on the entire group of lactating cows, when feeding 100 g of MBR per head per day, an increase in voluntary consumption of dry matter (+2 kg DM), an increase in average daily milk yield (+4.4 kg) per 1 head per day were noted . Return on investment in the activator of ruminal microflora by 1 ruble amounted to 12.7 rubles.

scholarly journals Effect of Chlorella Vulgaris and Japonochytrium sp. Microalgae Supplementation on Composition and Fatty Acid Profile of Goat Milk

2017 ◽  
Vol 65 (5) ◽  
pp. 1585-1593
Author(s):  
Klára Novotná ◽  
Milena Fantová ◽  
Lenka Nohejlová ◽  
Markéta Borková ◽  
Luděk Stádník ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
Milk Yield ◽  
Chlorella Vulgaris ◽  
Milk Fat ◽  
Goat Milk ◽  
Control Group ◽  
Basic Composition ◽  
Experimental Group

The aim of this study was to investigate the effect of two species of the microalgae on the milk yield, the basic composition and the fatty acid profile of goat milk, with focus on n‑3 fatty acids. Forty‑five White short‑haired goats were randomly allocated to three groups; the control group (C) with no supplementation microalgae to the diet. The first experimental group (Ch) was supplemented with Chlorella vulgaris and second experimental group (J) has been supplemented with Japonochytrium sp. The Japonochytrium supplementation negatively affected milk yield, but the amount of milk fat (+0.1 %; +0.45 %) and solids‑not‑fat (+0.27 %; +0.86 %) were higher than in group C and Ch. The amount of polyunsaturated (5.527 % ± 0.378) and saturated (71.560 % ± 0.861) fatty acids was also highest in group J. An increase of C20:4, C20:5 was detected in J and Ch, and the concentration of C22:6 was highest in group J (+0.019 %; P < 0.001).

scholarly journals Effects of Saccharomyces cerevisiae Culture on Ruminal Fermentation, Blood Metabolism, and Performance of High-Yield Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2401
Author(s):  
Xiaoge Sun ◽  
Yue Wang ◽  
Erdan Wang ◽  
Shu Zhang ◽  
Qianqian Wang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dairy Cows ◽  
Milk Yield ◽  
Inflammatory Cytokines ◽  
Rumen Fluid ◽  
Fat Content ◽  
High Yield ◽  
Control Group ◽  
And Performance ◽  
Basal Ration

High-yield dairy cows with high-concentrate diets are more prone to experiencing health problems associated with rumen microbial imbalance. This study assessed the effects of Saccharomyces cerevisiae culture (SC), a food supplement, on ruminal pH, volatile fatty acid (VFA), inflammatory cytokines, and performance of high-yield dairy cows. Forty Holstein cows with similar characteristics (e.g., milk yield, days of milk, and parity) were randomly divided into two groups: an experimental group fed the basal ration supplemented with the SC of 100 g of SC per cow per day (hour, SC group), and a control group fed the same basal ration diet without SC (i.e., CON group). On average, the supplementation of SC started at 73 days of lactation. The experimental period lasted approximately 70 days (from 18 January to 27 March 2020), including 10 days for dietary adaptation. Milk yield was recorded daily. Rumen fluid and milk samples were collected after 2 h of feeding in the morning of day 0, 15, 30, and 60. The data showed that rumen pH increased (p < 0.05) when cows were provided with SC. On average, the cows in the SC group produced 1.36 kg (p < 0.05) more milk per day than those in the CON group. Milk fat content of cows in the SC group was also higher (4.11% vs. 3.96%) (p < 0.05). Compared with the CON group, the concentration of acetic acid in the rumen fluid of dairy cows in the SC group was significantly higher (p < 0.05). There were no differences (p > 0.05) found in milk protein content and propionic acid between groups. The SC group had a tendency increase in butyric acid (p = 0.062) and total VFA (p = 0.058). The result showed that SC supplementation also enhanced the ratio between acetic and propionic. Most of the mean inflammatory cytokine (IL-2, IL-6, γ-IFN, and TNF-α) concentrations (p < 0.05) of the SC group were lower than CON group. This study demonstrated that high-yield cows receiving supplemental SC could produce more milk with higher fat content, have higher rumen acetate, and potentially less inflammatory cytokines.

Milk production, peripartal liver triglyceride concentration and plasma metabolites of dairy cows fed diets supplemented with calcium soaps or hydrogenated triglycerides of palm oil

2009 ◽  
Vol 77 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Roland G Karcagi ◽  
Tibor Gaál ◽  
Piroska Ribiczey ◽  
Gyula Huszenicza ◽  
Ferenc Husvéth
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Palm Oil ◽  
Milk Fat ◽  
Post Partum ◽  
Negative Energy ◽  
Plasma Metabolites ◽  
Control Group ◽  
Liver Triglyceride

The aim of the study was to test the effect of rumen-inert fat supplements of different chemical forms or containing different unsaturated/saturated (U/S) fatty acid contents on milk production, milk composition and liver and blood metabolic variables of high-yielding dairy cows in the peripartal period. Thirty Holstein-Friesian dairy cows were divided into three equal groups and fed a corn silage-based diet, without fat supplementation (control) or supplemented with 11·75 MJ NEl per day of calcium soaps of palm oil fatty acids (CAS; U/S=61/39) or with 11·75 MJ NEl per day of hydrogenated palm oil triglyceride (HTG; U/S=6/94). Each diet was fed from 25±2 d prior to the expected calving to 100±5 d post partum. Compared with the control, both CAS and HTG supplementation resulted in an increase of the average milk yield. Milk fat content and fat-corrected milk yield were higher in the HTG group but lower in the CAS group than in the control group. In all groups liver triglyceride concentrations (TGL) increased from 15 d prepartum to 5 d post partum, and then decreased thereafter. At 5 d TGL was lower in the HTG group than control or CAS cows. No significant differences were detected in TGL among dietary treatments at 15 d prepartum and 25 d post partum. Higher plasma glucose and insulin and lower non-esterified fattay acids and β-hydroxybutyrate concentrations and aspartate aminotransferase activity were measured in the HTG group than in the control or CAS groups at 5 d or 25 d post partum. Our results show that HTG may provide a better energy supply for high-yielding dairy cows in negative energy balance than CAS around calving.

Benefits of accurately allocating feed on a daily basis to dairy cows grazing pasture

2005 ◽  
Vol 45 (4) ◽  
pp. 331 ◽  
Author(s):  
W. J. Fulkerson ◽  
K. McKean ◽  
K. S. Nandra ◽  
I. M. Barchia
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Linear Regression Analysis ◽  
Daily Basis ◽  
Grazing Pressure ◽  
Control Group ◽  
Milk Component ◽  
Supplementary Feed

Two experiments were conducted, each over several months, when cows grazed either ryegrass (September–November 2001) or kikuyu (February–March 2002) pastures, to assess the effects of accurately allocating feed on a daily basis to lactating Holstein–Friesian dairy cows. In each case, 28 cows were randomly stratified into 2 equal groups on the basis of milk and milk component yield, liveweight, age and days in lactation. The metabolisable energy requirements of the animals were estimated from standard established requirements. In each experiment, both groups of cows received the same amount of supplement over a period that was equivalent to a pasture regrowth cycle of 12–16 days. The control group received a set amount of supplements each day, while supplements fed to the adjusted group varied, dependent on pasture available. Available pasture was varied from 7 to 21 kg DM/cow.day (above a stubble height of 5 cm), to mimic the variation found on well-managed dairy farms. When pasture available was above the predicted requirement for cows in the adjusted group, pasture availability was restricted to predicted requirements and the extra milk that could be produced from the spared pasture was estimated. However, cows in the control group had the opportunity to eat more pasture if allocated more than required. This could result in more milk being produced, a gain in liveweight, and/or a higher post-grazing pasture residue (and hence potentially improve pasture regrowth). If less pasture than required was allocated to the control group, production could reduce or the cows might graze harder. Thus, in the control group the proportion of forage to supplement remained relatively constant, but intake varied in relation to pasture allocated, while for the adjusted group the total intake was kept relatively constant. In experiment 1 (ryegrass), the milk yield, percentage of milk fat and liveweight change of cows in the control and adjusted groups was not significantly different. However, the cows in the adjusted group produced 0.016 kg/cow.day more milk protein. As the control group ate 0.35 kg DM/cow.day more ryegrass pasture (P = 0.008) it is assumed that accurate daily allocation of feed improved feed efficiency. In experiment 2, the milk yield and percentage of milk protein of cows grazing kikuyu pastures was not significantly different between groups but the percentage of milk fat and covariate-corrected liveweight at the end of the experiment was higher in the control group than in the adjusted group. The pasture spared by cows in the adjusted group was predicted to produce 8.9% more milk when grazing ryegrass pasture and 12.3% when grazing kikuyu pasture. Linear regression analysis of pasture on offer on post-grazing pasture residue was not significant for the cows in the adjusted group but was significant for the control group cows when grazing either pasture, indicating success in accurately allocating supplementary feed to maintain a constant grazing pressure. The results of this study should assist dairy farmers in deciding whether the effort required to allocate feed accurately to dairy cows on a daily basis, is worthwhile.

scholarly journals The needs and characteristics of feeding dairy cows

2017 ◽  
Vol 19 (79) ◽  
Author(s):  
L. G. Levitskaya
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Positive Impact ◽  
The Body ◽  
Control Group ◽  
Milk Productivity ◽  
Feed Management ◽  
Feed Costs ◽  
Intensive Course ◽  
Experimental Group

The influence of feed with different contents in them in certain ratios lekarstvennogo and insoluble protein on the body of dairy cows. In particular, digestion in the rumen, digestibility of nutrients of feed, milk productivity and milk quality. Identify the differences in some indicators of digestion in the rumen of cows, the digestion of protein (carrying experience) on the background of the use of the experimental feed. In this grain component was subjected to extrusion and introduced maize, the protein which vikoristannya. Because of this, reduced the solubility of protein from 38% to 29% that is 9%. As a result, extended the time of their digestion in the rumen. So scar microflora sufficiently transformed the ammonia available for absorption of microbial protein, which has a positive impact on the processes of digestion and assimilation of nutrients and contributed to improving milk productivity in cows. The structure of the diets of both groups was identical and included: roughage is 12.5% juicy – 75.0%, concentrated to 12.5%. One fodder unit in the control group had 90.2 g digestible protein in experimental 89.6 g Sharapodinov attitude in control and experimental diets is 0,98. The ratio of mineral substances: CA : P in the control and experience is 1.6 : 1, N : S respectively of 7.3 and 6.9. On a more intensive course of metabolic processes show physiological and biochemical parameters in animals of the experimental group. Namely: reducing the amount of ammonia and the increase in the number of bacteria in the rumen. The composition of basic ration (PR), which included feed management. All experimental animals during the preparatory period received the same diet. Research in accounting period II (experienced) group – experimental mixed fodder in which to reduce the solubility of the protein component of grain (oats, barley, wheat) extrudable and additionally introduced maize grain (15%), the control group received commercial feed. Found that feeding the experimental feed, improved nitrogen metabolism in the organism of cows, heifers, which ultimately increased milk yield by 4.4% (vs. 26.3 to 25.2 kg). The reduction in feed costs and increase milk yield in cows of the experimental group resulted in a reduction of cost of 1 quintal of milk 27.1 UAH. and improve profitability by 2.3% against the control.

scholarly journals The dynamics of humoral protection factors in broilers under the conditions of probiotic preparations application

2018 ◽  
Vol 20 (83) ◽  
pp. 264-267
Author(s):  
M.M. Romanovych
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Broiler Chickens ◽  
Intestinal Microflora ◽  
Natural Resistance ◽  
Control Group ◽  
Free Access ◽  
Functional Impairments ◽  
Yeast Saccharomyces Cerevisiae ◽  
Group I ◽  
Experimental Group

The health of farm poultry depends on the balance between normal and potentially pathogenic intestinal microflora. Any changes in this equilibrium are accompanied by functional impairments, which, in turn, lead to a decrease in productivity. The use of probiotics helps to avoid imbalance of intestinal microflora and death of young animals. In this connection, it is relevant to study the effects of probiotic drugs to improve the vitality and immune function of the poultry. The purpose of the research was to clarify the effect of BPS-44 and different amounts of yeast Saccharomyces cerevisiae on the activity of the humoral level of natural resistance in chicken broilers. Experiments were carried out on broiler chicks-308 grown in the Fedyuk M farm in the Zolochiv district of Lviv oblast. The maintenance of the chickens was cellular with free access to feed and water. Technological parameters of broiler cultivation (temperature and light regime) were in accordance with the norms of ONTP-2005. Experiments were conducted in 4 groups of broiler chickens of 100 chicks in each according to the scheme: the control group was fed with the standard feed (SC) according to the existing norms recommended for the ROSS-308 cross; the experimental group I in addition to the SC received a probiotic BPS-44 (registration certificate No. 2154-04-0254-06 dated November 24, 2006), based on the production strain of bacteria Bacillus subtilis ssp. subtilis 44-p, dose 0.21 g/kg, the experimental group II – 1% yeast Saccharomyces cerévisiae; the experimental group III of chickens – 2% yeast Saccharomyces cerévisiae. Blood was taken from chickens in order to carry out immunological research at different age intervals: 11, 27, 34 and 41 days of age. Blood serum was determined by bactericidal and lysozyme activity and the content of circulating immune complexes. The stimulatory effect of BPS-44 and yeast Saccharomyces cerеvisiae in the composition of mixed fodder for broiler chickens on the dynamics of the formation of humoral protection factors in the bird organism was established. At the same time, in the conditions of use of yeast Saccharomyces cerэvisiae in the composition of mixed fodder, a higher level of indices of the humoral level of non-specific resistance was recorded, than the preparation BPS-44.

Influence of the Amyrasol R-Z mineral lick briquette on biochemical parameters of blood in dairy cowsInfluence of the Amyrasol R-Z mineral lick briquette on biochemical parameters of blood in dairy cow

2020 ◽  
pp. 13-14
Author(s):  
Ayub Yu. Aliev ◽  
◽  
Karine A. Karpushchenko ◽  
Abdulgamid A. Aliev ◽  
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Biochemical Parameters ◽  
Fat Content ◽  
Control Group ◽  
Milk Productivity ◽  
Basic Diet ◽  
The Republic ◽  
Experimental Group ◽  
Mineral Lick

The data obtained as a result of the use of a mineral lick briquette Amirasol R-Z in the diets of dairy cows are analyzed in the article. Authors studied the influence of the mineral lick briquette Amirasol R-Z on the biochemical parameters and milk productivity of dairy cows. The experiments were carried out on dairy cows of the red steppe breed in the conditions of the plain zone of the Republic of Dagestan. Two groups of dairy cows were formed (10 heads in each). According to the scheme of the experiment, the control group received a basic diet, the experimental group received the basic diet and the mineral lick briquette Amirasol R-Z. Each cow of the experimental group was given individually one lick briquette in a dose of 4 kg during 90 days. Researches and data processing were carried out according to generally accepted methods. Blood was taken from the experimental animals from the jugular vein for laboratory studies at the end of the experiment. The milk productivity and fat content of milk were taken into account by carrying out monthly control milk yields. At the same time, the positive effect of a lick briquette on the biochemical parameters of blood has been proven: reserve alkalinity, glucose, macro- and microelements, the activity of the copper-containing enzyme ceruloplasmin and milk productivity. A significant increase in the blood of glucose concentration by 12.93% in the cows of the experimental group, the level of reserve alkalinity by 17.66%, the concentration of macroelements and microelements, milk productivity by 5.7%, milk fat by 2.77% compared with the control group. The use of the mineral lick briquette Amirasol R-Z in the diets of dairy cows helps to prevent macro- and microelementoses, increase milk productivity, milk fat content, nonspecific immunity and obtain of healthy calves.

505 Late-Breaking: Ruminal Microbiota, Inflammation Cytokines, and Performance of High-yield Dairy Cows Supplemented with Saccharomyces Cerevisiae Culture

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 179-180
Author(s):  
Xiaoge Sun ◽  
Shu Zhang ◽  
Erdan Wang ◽  
Na Lu ◽  
Wei Wang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dairy Cows ◽  
Milk Yield ◽  
Relative Abundance ◽  
Milk Fat ◽  
Rumen Fluid ◽  
High Yield ◽  
Rumen Ph ◽  
And Performance ◽  
Inflammation Cytokines

Abstract Dramatic increases in milk yields in recent decades have created challenges in terms of rumen pH and microbial health which ultimately impact dairy cow health. The objective of this study was to assess the effects on ruminal pH, Volatile Fatty Acid (VFA), microbiota, inflammation, and performance of high-yield dairy cows by supplementing Saccharomyces cerevisiae culture (SC). Forty Holstein cows were divided into two groups based on their milk yield, days of milk, and parity fed the same basal ration diet that did or did not contain 100 g of SC /cow per day. Individual dry matter intake (DMI) and milk yield were recorded each day. Rumen fluid and milk samples were collected after 2 hours of morning feeding at intervals of 15 days during the experiment period. The data showed that rumen pH was increased by 0.19 (P = 0.09) when SC was supplemented than no SC was provided. SC-supplemented cow consumed 0.28 kg (P &lt; 0.05) extra DM/d. Those supplemented with SC produced 1.36 kg (P &lt; 0.05) more milk/cow per day than did non-supplemented cows. Milk fat percentage was higher (4.11 vs. 3.96%) for cows receiving SC. There were no differences in milk protein percentage. Rumen fluid VFA concentration was not statistically affected by SC but was numerically higher acetic and lower propionic for supplemented cows. The blood of the SC group with lower inflammation cytokines and somatic cell count (SCC). SC-supplemented cows had a greater relative abundance of Prevotellaceae, Succinivibrionaceae, Fibrobacteraceae, Lactobacillaceae, and lower relative abundance of Spirochaetaceae, Methanobacteriaceae, Enterobacteriaceae than the unsupplemented cows. It had greater functions on xylanolysis, fermentation, cellulolysis in the rumen in terms of the KEGG function prediction analysis. This study demonstrated that high-yield lactation cows receiving supplemental SC produced more milk and potentially reduced the inflammation and enhanced rumen cellulolysis bacteria growth.

Effect of parenteral administration of Selenium and vitamin E on health status of mammary gland and on selected antioxidant indexes in blood of dairy cows

2014 ◽  
Vol 17 (2) ◽  
pp. 217-223 ◽  
Author(s):  
F. Zigo ◽  
Z. Farkasóvá ◽  
J. Eleko ◽  
M. Lapin ◽  
M. Chripková ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Health Status ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Clinical Mastitis ◽  
Parenteral Administration ◽  
Control Group ◽  
Blood Samples ◽  
Experimental Group

Abstract This study focuses on the effect of parenteral administration of Selenium (Se) and vitamin E on concentration of Se in plasma and the activity of glutathione peroxidase (GPx) in the blood of dairy cows during peripartal period and their effect on the reduction of clinical mastitis. From a 220 individuals Holstein herd in a two-four lactation-gestation cycle the control group (C), 1st (D1) and 2nd (D2) experimental group were selected. Every group consisted of 15 cows in the last phase of the pregnancy. All cows were fed with the diet containing 0.1 mg of Se per kg/DM. The blood samples from vena jugularis were collected approximately 21 days before calving (control sampling), 3 days, 12 days and 21 days after calving. On the day of control sampling and 12 days before calving in D1 group, cows were injected subcutaneously in the sprescapular region with preparation Selevit inj. a.u.v. at the doses of 48.4 mg/head of Se, and 550 IU/head of α-Tocoferol (α-Toc). In D2 group, cows were injected by the same preparation only on 21th day before calving with the same doses of Se and α-Toc. The increase in the concentration of Se in the plasma and activity GPx in blood in D1 group on the 3rd day and 12th day after calving were determined. Increase in plasmatic concentrations α-Toc on 3rd day after calving and reduction of occurrence of clinical mastitis (13.3%) as compared with control group were found

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