scholarly journals Influence of sulpiride treatment on the level of prolactin and immunoglobulins in the peripheral blood of mares during the postpartum period

2017 ◽  
Vol 65 (3) ◽  
pp. 417-428 ◽  
Author(s):  
Anna Migdał ◽  
Łukasz Migdał ◽  
Agata Zagrajczuk ◽  
Joanna Kochan ◽  
Agnieszka Nowak ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Blood Plasma ◽  
Peripheral Blood ◽  
Postpartum Period ◽  
Experimental Period ◽  
Control Group ◽  
Immunoglobulin Level ◽  
Lactogenic Hormone ◽  
Experimental Group ◽  
In Blood Plasma

The aim of this study was to determine the effect of increased levels of prolactin (PRL) on the concentration of immunoglobulins in the blood, colostrum and milk of mares. The study was conducted on 12 mares of the Polish Pony breed (6 in the control and 6 in the experimental group). To induce hyperprolactinaemia in mares of the experimental group, 750 mg sulpiride was administered orally once a day. The initial PRL concentration was 52.22 ± 11.21 ng/ml in the control group and 49.39 ± 10.12 ng/ml in the experimental group. In the subsequent days, the concentration of PRL dynamically changed. Statistical analysis showed highly significant differences (P < 0.01) between the groups. The concentration of immunoglobulins in the blood plasma was at the same level during the experimental period (32.97–29.08 mg/ml in the experimental group and 28.60–18.11 mg/ml in the control group). Statistical analysis showed highly significant differences between the groups in blood plasma immunoglobulin level (P < 0.01). The highest immunoglobulin concentration was obtained within 12 h after parturition in the control and the experimental group (23.49 ± 2.12 mg/ml and 26.94 ±1.72 mg/ml, respectively). The lowest values were obtained on day 12 after parturition in the experimental group (10.15 mg/ml ± 1.47 mg/ml) and on day 7 after parturition in the control group (14.30 mg/ml ± 2.48 mg/ml). In conclusion, this study did not provide evidence that the lactogenic hormone prolactin is involved in the transfer of immunoglobulins into the colostrum in horses.

scholarly journals The effect of silicon compounds on hematological indicators and content of lipids in blood of rabbits

2018 ◽  
Vol 20 (92) ◽  
pp. 190-196 ◽  
Author(s):  
A. I. Ivanitskaya ◽  
Ya. V. Lesyk
Keyword(s):  
Body Weight ◽  
Blood Plasma ◽  
Fractional Composition ◽  
The Body ◽  
Control Group ◽  
Free Access ◽  
Animal Blood ◽  
Preparatory Period ◽  
Experimental Group ◽  
In Blood Plasma

The aim of the study was to find out the effect of casting of citrate nanosіlicon absorption and metasilicate sodium on the hematological parameters and lipid content and their fractional composition in blood plasma preparatory period 10 days prior to insemination and 20 days of lactation. The research was carried out on 60 rabbits of the Hyla hybrid, divided into three groups (control and two experimental), with 20 animals in each. The control animals were fed without limitation full-grain granulated feed with free access to water. Animals of the first experimental group (E-I) fed the diet of the control group and during the course of the day poured out the silicon citrate obtained using the nanotechnology method, 50 mkg Si/kg body weight with water. Samples of the second experimental group (E-II) feed the diet of the control group and set the sodium metasilicate (Na2SiO3H2O) 2.5 mg Si/kg body weight with water. The trial lasted 95 days, including the preparatory period of 10 days, the trial – 85 days. In the preparatory period of 10 days from the beginning of the study and in a trial of 20 days of lactation (65 days of supplements) in the rabbits, samples of blood from the marginal ear vein were taken. Hematologic studies were performed using the automatic hematologic analyzer “Orphee Mythic 18”, in the blood plasma, the content of total lipids and their fractional composition were determined. It was established that the number of erythrocytes and hemoglobin concentration in the blood of the rabbits of experimental group, which were given silicon citrate, were respectively higher by 15.2% (P < 0.05) and 12.0% (P < 0.01) by 20 days lactation versus control. This may indicate a more pronounced effect of the organic compound of silicon on the hematopoietic function of the body of the rabbits. According to white blood, the differences from control were found during the presentation of supplements with higher (P < 0.05) changes in 20 days of lactation in animal blood and experimental group. It was noted that the content of triacylglycerols in plasma of blood of groups I and II was lower by 31.2 and 32.8%, respectively (P < 0.05) compared to control. In blood plasma of animals in experimental groups an increase in the fractions of phosphatidylcholine, sphingomyelin and lysolecetin was observed compared to control. Such a redistribution was due to the reduction of the fractions of phosphatidic acid, phosphatidyl ethanolamine and phosphatidylinositol, and apparently, is associated with changes in the activity of the enzymes concerned. Hematological studies and determination of the fractional composition of lipids and phospholipids of blood plasma of the rabbits show positive changes that contribute to the metabolic accumulation of energy and plastic components of their organism.

scholarly journals Influence of toys on behavioural patterns of pigs and their association with the concentration of serotonin in blood plasma

2020 ◽  
Vol 11 (1) ◽  
pp. 146-150 ◽  
Author(s):  
A. V. Lykhach ◽  
V. Y. Lykhach ◽  
M. B. Shpetny ◽  
O. H. Mykhalko ◽  
S. V. Zhyzhka
Keyword(s):  
Weight Gain ◽  
Blood Plasma ◽  
Live Weight ◽  
Daily Weight Gain ◽  
Video Observation ◽  
Control Group ◽  
Free Access ◽  
Group I ◽  
Experimental Group ◽  
In Blood Plasma

Improvement of welfare of piglets is one of the key factors which underlie the effectiveness of producing pork and the profitability of pig farming. This article offers a solution to the main industrial problem – creation of comfortable maintenance conditions for pigs using toys which satisfy their ethological needs. Piglets used in the experiment were kept in the swine complex of agricultural production of Agrofirm Mig-Service-Agro Cooperative in Novoodesky district of Mykolaiv Oblast (Ukraine). The objective of the study was assessment of the effect of toys on the behaviour of piglets, level of concentration of hormone serotonin in blood plasma and their energy of growth. The experiment was performed on 120 weaned piglets aged 28 days, which were divided according to the principle of analogues with 60 individuals in each group: I – the control group, the animals were kept with no toys; II – the experimental group, where the animals had toys. During the experiment chronometric monitoring of the piglets’ behavioural acts was carried out using video observation, we extracted blood samples for determination of the content of the hormone serotonin using the method of highly-efficient fluid chromatography and assessed the growth of the piglets. After the end of the experiment, we determined that the presence of toys reduced the intragroup aggression, which was accompanied by decrease in the duration of fights and almost three-fold increase in playing behaviour. Among piglets which had access to toys, the number of individuals observed to bite tails and ears decreased from 11 to 3. In the period of the study, the concentration of serotonin in the blood plasma of piglets who had free access to toys was 10 times higher compared with animals of the control group. Change in the behaviour patterns among the animals of the experimental group led to significant differences regarding the parameters of growth, where probable increase in the live weight at the age of 90 days was observed, which was associated with significant increase in their mean daily weight gain compared with the animals of the control group. This study confirms that use of toys for piglets during growth completion can prevent features of aggression and abnormal forms of stereopathy within groups, increase the live weight and mean daily weight gain and improve emotional condition, which affects the welfare of pigs.

scholarly journals VISFATIN LEVEL DISTURBANCES IN PERIPHERAL BLOOD OF PATIENTS WITH DIABETIC NEPHROPATHY

2014 ◽  
pp. 25-30
Author(s):  
I.I. Topchii ◽  
P.S. Semenovykh ◽  
V.YU. Galchinskaya ◽  
N.V. Yefimova
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Plasma ◽  
Peripheral Blood ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Visfatin Level ◽  
Type 2 Diabetic ◽  
In Blood Plasma

 Introduction. Recent studies suggest that visfatin participates in pathogenesis of vascular diabetic complications, in particular diabetic nephropathy (DN). The aim of the present research - definition of visfatin level in peripheral blood of type 2 diabetic patients taking to account renal function disturbances and body mass index (BMI). Materials and methods. 94 type 2 diabetic patients with different stages of DN and 10 healthy subjects (control group) were observed. Visfatin concentration in blood plasma was determined using immunoassay kit. Results. An essential increase of visfatin level in blood plasma took place already in initial stages of the DN. Progressing of the disease was accompanied by more expressed growth of visfatin concentration. In patients with high BMI substantial increase of visfatin level when compared with those with normal IMT was determined. We established strong correlations between visfatin concentrations, urinary albumin levels and blood creatinine concentrations and negative correlations with glomerular filtration rate. Conclusions: Our findings testify that visfatin level displays a functional kidney state and may be used as addition to traditional methods of patients examination.

scholarly journals INFLUENCE OF ALIMENTARY INSUFFICIENCY OF CALCIUM, MAGNESIUM AND IODINE ON RAT VITAMIN STATUS

2021 ◽  
Vol 22 (1) ◽  
pp. 52-59
Author(s):  
O.A. Vrzhesinskaya ◽  
◽  
V.M. Kodentsova ◽  
N.A. Beketova ◽  
O.V. Kosheleva ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Vitamin B1 ◽  
Male Rats ◽  
Molar Ratio ◽  
Vitamin Status ◽  
Iron And Zinc ◽  
Calcium Magnesium ◽  
Increased Activity ◽  
Experimental Group ◽  
In Blood Plasma

The diet of the majority of the population of Russia is characterized by an insufficient content of a number of micronutrients, including macro- and micronutrients. The aim of the study is to characterize in a rat model experiment the effect of combined nutritional deficiency of calcium, magnesium and iodine on the availability of other mineral substances, vitamin metabolism and biochemical parameters blood plasma. The lack of minerals in growing Wistar male rats with an initial body weight (51.4 ± 0.5) g was created for 23 days by halving of a calcium, magnesium and iodine in the salt mixture. Alimentary deficiency of calcium, magnesium and iodine in growing rats significantly and multidirectionally affected the metabolism of other minerals and vitamins, as well as a number of diagnostically significant indicators of blood and urine. In rats of the experimental group, the liver concentration of α-tocopherol, vitamin B1 and retinol palmitate decreased, the concentration of iron and zinc increased, the copper content in the whole brain decreased significantly. The molar ratio of γ- and α-tocopherols in blood plasma was increased, the concentration of the circulating form of vitamin D decreased. The ratio of AlAT / AsAT (de Ritis coefficient) markedly decreased due to increased activity of AlAT. Phosphate reabsorption decreased slightly. Increased urinary excretion of riboflavin was accompanied by a decrease in its concentration in blood plasma. The conclusion that insufficient intake of calcium, magnesium and iodine may disturb vitamin-mineral status of organism has been made.

scholarly journals The effect of iodine and strumigens long-term foodborne intake on histometrical parameters of thyroid gland in gimmers

2013 ◽  
Vol 61 (5) ◽  
pp. 1365-1369
Author(s):  
Zdeněk Peksa ◽  
Jan Trávníček ◽  
Roman Konečný ◽  
František Jelínek ◽  
Hana Dušová ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Dry Matter ◽  
Experimental Period ◽  
Rapeseed Meal ◽  
Iodine Intake ◽  
Control Group ◽  
High Iodine ◽  
Average Size ◽  
Experimental Group

In 2010 and 2011 two experiments on gimmers of Šumava mountain sheep were performed. The first experiment was carried out on 12 animals (experimental period was 11 months); control group was fed with 3 mg I*kg−1 in dry matter per day and experimental group was fed with 5 mg I*kg−1 in dry matter per day. The second experiment lasted 10 months and it was carried out on 12 animals. Feed ration for control group contained 10 mg I*kg DM−1. Feed ration for experimental group contained 10 mg I*kg DM−1 too; moreover it included rapeseed meal and 1 g of sodium nitrate. The aim of the first experiment was focused on impact of high iodine intake on structure of thyroid gland. The aim of the second experiment was to discover effect of strumigens during high iodine intake on structure of thyroid gland. The animals were slaughtered after the experiment and there was executed the dissection of thyroid gland. The samples of thyroid gland were processed during classic paraffin method and dyed with haematoxylin and eosin. For finding of histometrical parameters was used program Leica IM 500 Version 4.0. The length, the width and the area of follicles were measured. The follicles were divided into three groups after this procedure (by the length); in each group were measured 20 thyreocytes. In group with intake 5 mg I*kg DM−1 (experiment from year 2010): higher weight of thyroid (p < 0.01), significant higher (p < 0.05) proportional representation of large and medium follicles, significantly (p < 0.05) higher average size of follicles, demonstrably lower (p < 0.05) height of epithelium were found. There were not found any differences in monitored parameters between the groups from experiment which was carried out during the year 2011. After comparing results from the both experiments significantly higher percentage representation of large follicles a significantly lower representation of small follicles, distinctly higher average size and higher height of epithelium in all size categories, in groups with iodine intake 10 mg I*kg DM−1was found (from second experiment – year 2011).

scholarly journals Great Obstetrical Syndromes In G20210a Mutation Carriers Associated With High Prothrombin Activity

2020 ◽  
Author(s):  
Mariya Nikolaeva ◽  
Andrey Pavlovich Momot ◽  
Marina Sabirovna Zainulina ◽  
Natalia Nikolaevna Yasafova ◽  
Irina Alekseevna Taranenko
Keyword(s):  
Blood Plasma ◽  
Venous Blood ◽  
Threshold Value ◽  
Main Group ◽  
Trophoblast Invasion ◽  
Control Group ◽  
G20210a Mutation ◽  
Factor Ii ◽  
Invasion Waves ◽  
In Blood Plasma

Abstract Objective: to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes.Material and methods: A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out.Results: In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR).Conclusion: The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).

scholarly journals THE EFFECT OF SCIENCE LEARNING MODEL, ENVIRONMENT, TECHNOLOGY, AND SOCIETY ASSISTED BY ENVIRONMENTAL MEDIA ON SCIENCE KNOWLEDGE COMPETENCE OF GRADE V ELEMENTARY SCHOOL STUDENTS OF SRIKANDI CLUSTER EAST DENPASAR IN ACADEMIC YEAR 2017/2018

2018 ◽  
Vol 2 (2) ◽  
pp. 100
Author(s):  
Putu Suwi Arista dewi ◽  
I N Jampel ◽  
I N L Jayanta
Keyword(s):  
Statistical Analysis ◽  
Science Learning ◽  
Learning Model ◽  
Choice Test ◽  
Control Group ◽  
Class V ◽  
Environmental Media ◽  
Significant Difference ◽  
Experimental Group ◽  
Model Environment

This study aims to determine the effect of Science Learning Model, Environment, Technology, Society assisted Environmental media on Science Competence of grade V students. The type of this research was quasi experiment with non-equivalent group design. The population in this study was the entire class V in the Srikandi cluster East Denpasar amounted to 362 students. The sample of this research was class V of SDN 5 Sumerta were 28 students as experimental group and class V of SDN 10 Sumerta were 28 students as control group. Data collected with multiple choice test instruments, then was analyzed using descriptive statistical analysis and inferential statistical analysis (t-test). Based on the data analysis, tcount = 2.500 and ttabel (5% significance level) = 2,000. This means that tcount = 2,500> ttabel = 2,000. There was a significant difference of science competence between groups of students who was taught by science learning model, Environment, Technology, Society assisted Environmental media and group of students which is learn through conventional learning. The average value of science competence of the experimental group ( ) is 79.00 while the mean score of control group students ( ) is 74.07. This means =79,00 > =74,07, so it can be concluded that the application of learning model of Science, Environment, Technology, Society assisted Environmental media influenced the competence of science. Based on the results of this study it is suggested that the results of this study can be used as a relevant study, especially as supporting the next research.

scholarly journals Therapeutic Effect of Oral Administration and External Therapy of Traditional Chinese Medicine on Cervical Tuberculous

2019 ◽  
Vol 3 (3) ◽  
Author(s):  
Guoqiang Yang ◽  
Jin Zhang
Keyword(s):  
Statistical Analysis ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Oral Administration ◽  
Total Effective Rate ◽  
Statistical Significance ◽  
Effective Rate ◽  
Control Group ◽  
Treatment Data ◽  
Experimental Group

Objective: To analyze the clinical effect and value of oral administration and external therapy of traditional Chinese medicine for treatment of cervical tuberculous lymphadenopathy. Methods: A total of 56 patients with cervical tuberculous lymphadenopathy admitted to our hospital from January 1 to January 2018 were recruited. By using double-blind method, the patients were divided into control group (n=28) and experimental group (n=28). Control group comprised of patients with conventional anti-tuberculosis treatment, while the experimental group comprised of patients treated with oral administration and external therapy of traditional Chinese medicine. Total effective rate, incidence of complication and level of T lymphocyte subsets were compared between the two groups of cervical tuberculous lymphadenopathy patients. Results: Post-treatment data of total effective rate, complication rate, CD3+, CD4+, CD8+, CD4+/CD8+ of the experimental group were compared with the control group. P<0.05; statistical analysis showed statistical significance. Post-treatment data of CD3+, CD4+, CD8+, CD4+/CD8+ of both control and experimental groups were compared with pre-treatment data. P<0.05; statistical analysis showed statistical significance. Conclusion: Oral administration and external therapy of traditional Chinese medicine possesses significant effect in treatment of cervical tuberculous lymphadenopathy.

scholarly journals Dynamics of the Antioxidant System and Endogenous Intoxication in Patients Being Treated for Microbial Eczema

2019 ◽  
Vol 26 (4) ◽  
pp. 56-65
Author(s):  
М. M. Tlish ◽  
Е. К. Popandopulo
Keyword(s):  
Molecular Weight ◽  
Blood Plasma ◽  
Antioxidant System ◽  
Catalase Activity ◽  
Reduced Glutathione ◽  
Control Group ◽  
Low Molecular Weight ◽  
Antioxidant Defence ◽  
Endogenous Intoxication ◽  
Experimental Group

Aim. The paper studies the dynamics of the indicators defi ning the antioxidant system (AOS) and endogenous intoxication in patients with microbial eczema (ME) prior to and following treatment.Materials and methods. The experimental group consisted of patients with ME (n=30) and the control group comprising somatically healthy individuals (n=30). In this study, we determined the components of the antioxidant defence system in blood plasma and erythrocyte suspension, as well as assessed endogenous intoxication.Results. The study revealed an imbalance of indicators refl ecting the severity of oxidative stress and endogenous intoxication in patients suffering from ME. Prior to receiving treatment, the experimental group, as compared to the control group, exhibited an increase in catalase activity (CAT) up to 35.93 [32.50; 38.22] mmol / (min • l) (p < 0.001) and glutathione peroxidase (GPx) up to 1194.27 [1069.50; 1375.78] μmol / (min • l) (p < 0.001). In addition, there was a decrease in the activity of superoxide dismutase (SOD) to 13.28 [11.35; 14.10] u/l (p < 0.001), glutathione reductase (GR) to 501.46 [479.77; 542.52] mmol / (min • l) (p < 0.001) and the concentration of reduced glutathione to 1.64 [1.59; 1.78] μmol / ml (p = 0.043). We observed no statistically signifi cant changes in the total antioxidant activity (TAA) of blood plasma (0.59 [0.55;0.62] mg/l; р = 0.375) and the sulphhydryl groups (0.44 [0.41;0.47] ODU; р =0.111). The patients with ME exhibited the syndrome of endogenous intoxication (SEI), accompanied by an increase in the content of medium and low molecular weight molecules in plasma to 12.19 [11.20; 13.37] arb. u. (p < 0.001), with their content in erythrocytes being relatively normal – 15.23 [14.26; 16.44] arb. u. (p = 0.005). Following the treatment, changes in the studied parameters were of a multidirectional character in relation to the control values. An increased level of CAT and GPx (р <0,001), as well as lower values of SOD and GR, continued to be observed (р < 0.001). The TAA in serum (p = 0.308), sulphhydryl groups (p = 0.111) and reduced glutathione (p = 0.107) did not differ signifi cantly from the control values. The treatment did not reverse the SEI: the content of medium and low molecular weight molecules continued to increase in both plasma and erythrocytes, as compared to the control values (p < 0.001 in both cases).Conclusion. The obtained data confi rm the failure of mechanisms underlying antioxidant defence in patients with ME. Despite the attempt of its compensation by increasing the catalase activity, SOD control values were still not reached. An increase in the GPx activity along with a decrease in GR was observed, which could have lead, in turn, to a decrease in the concentration of reduced glutathione. The failure of the antioxidant defence mechanisms is also indicated by an increase in the content of medium and low molecular weight molecules, as well as by the development of the SEI, resulting from the excessive formation of reactive oxygen species and the inability of the antioxidant system to neutralise them.

the effect of Needham’s Constructive Model on the achievement of the 2nd year intermediate students in Mathematics and their attitudes towards it

2021 ◽  
Vol 28 ◽  
Keyword(s):  
Statistical Analysis ◽  
Achievement Test ◽  
Control Group ◽  
Constructive Model ◽  
Partial Control ◽  
Intermediate Students ◽  
Iq Test ◽  
Attitude Towards Mathematics ◽  
Multiple Choice Items ◽  
Experimental Group

The study aims to identify the effect of Needham’s Constructive Model on the achievement of the 2nd year intermediate students in Mathematics and their attitudes towards it. The researchers used the experimental design of partial control for the control and experimental groups. The study sample is 68 students, where the experimental group is 34 students, and the control group is (34) students. The researchers prepared two scales as achievement test, which include (30) multiple choice items. Items psychometric properties and statistical analysis have been got, and a scale of attitudes towards mathematics that includes (30) items with three alternatives. Items reliability, validity, discrimination coefficients are got. The two groups were rewarded in the variables of age and previous achievement in mathematics, IQ test and attitudes scale. After applying the experiment and the two study tools on the study sample, the results showed that there were statistically significant differences at the level (0.05) in favour of the experimental group in achievement and attitude towards mathematics. Key words: Needham’s model attitudes toword mathematics

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