Investigation of probiotic potential of yeasts isolated from sourdoughs from different regions of Turkey

2021 ◽  
Author(s):  
Z. Alkay ◽  
E. Dertli ◽  
M.Z. Durak
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Salt Concentration ◽  
Probiotic Properties ◽  
High Antimicrobial Activity ◽  
Yeast Cultures ◽  
Probiotic Potential ◽  
Yeast Strains ◽  
Ft Ir ◽  
Saccaromyces Cerevisiae

Abstract In this study, 14 yeast cultures from 62 isolates from traditional sourdoughs collected from 6 different regions of Turkey were selected by FT-IR identification and characterised to reveal their probiotic properties. Four yeast strains were genotypically identified and compared with FT-IR identification. In all analyses, it was observed that mostly Saccaromyces cerevisiae strain exhibited high hydrophobicity, auto-aggregation feature, and all yeast isolates in this study showed tolerance to 0.3%, even salt concentration. In addition, all yeast strains were susceptible to anti-yeasts agents, although they were resistant to all antibiotics used in the study. All selected yeast isolates exhibited high antimicrobial activity against the Staphylococcus aureus. In conclusion, this study investigated the potential probiotic properties of yeast strains isolated from sourdough.

Identification and Evaluation of Probiotic Potential in Yeast Strains Found in Kefir Drink Samples from Malaysia

2019 ◽  
Vol 15 (7) ◽  
Author(s):  
Mohd Akmal Azhar ◽  
Mimi Sakinah Abdul Munaim
Keyword(s):  
Antimicrobial Activity ◽  
Probiotic Properties ◽  
E Coli ◽  
Probiotic Potential ◽  
Rdna Sequences ◽  
Yeast Strains ◽  
16S Rdna Sequences ◽  
Human Body Temperature ◽  
Probiotic Yeast ◽  
New Yeast

AbstractKefir drink is a source of probiotic microorganism with remarkable functional and technological properties. The objective of this work is to isolate yeast strains from Malaysian kefir drink and evaluate them for probiotic potentials. In the present study, nine strains of probiotic yeast were isolated from a Malaysian kefir drink and identified according to their 16S rDNA sequences. Furthermore, their probiotic potential was evaluated. The probiotic properties were tested for aspects of antibiotic susceptibility, antimicrobial activity, and gastrointestinal condition tolerance (pH and temperature). Five isolated strains, M3, Y5, Y9, Y11 and A1, showed good tolerance towards low pH condition while three strains, A1, M1, and M3, showed antimicrobial activity against E. coli, P. aeruginosa, and Salmonella sp. Most isolates were resistant to penicillin, streptomycin, and ampicillin, and grew well at human body temperature. The result of this test indicates that the yeast strains isolated from Malaysian kefir drink have excellent potential for use as probiotics in various products. Lastly, kefir milk is one of the excellent source of probiotic yeast strains and could be used as a new yeast probiotic formulation or in food supplements.

scholarly journals Proliferation of Antibiotic-Producing Bacteria and Concomitant Antibiotic Production as the Basis for the Antibiotic Activity of Jordan's Red Soils

2009 ◽  
Vol 75 (9) ◽  
pp. 2735-2741 ◽  
Author(s):  
Joseph O. Falkinham ◽  
Thomas E. Wall ◽  
Justin R. Tanner ◽  
Khaled Tawaha ◽  
Feras Q. Alali ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Incubation Period ◽  
Micrococcus Luteus ◽  
Antibiotic Production ◽  
Antibiotic Activity ◽  
Skin Infections ◽  
High Antimicrobial Activity ◽  
Methanol Extracts ◽  
Red Soils

ABSTRACT Anecdotes, both historical and recent, recount the curing of skin infections, including diaper rash, by using red soils from the Hashemite Kingdom of Jordan. Following inoculation of red soils isolated from geographically separate areas of Jordan, Micrococcus luteus and Staphylococcus aureus were rapidly killed. Over the 3-week incubation period, the number of specific types of antibiotic-producing bacteria increased, and high antimicrobial activity (MIC, ∼10 μg/ml) was observed in methanol extracts of the inoculated red soils. Antibiotic-producing microorganisms whose numbers increased during incubation included actinomycetes, Lysobacter spp., and Bacillus spp. The actinomycetes produced actinomycin C2 and actinomycin C3. No myxobacteria or lytic bacteriophages with activity against either M. luteus or S. aureus were detected in either soil before or after inoculation and incubation. Although protozoa and amoebae were detected in the soils, the numbers were low and did not increase over the incubation period. These results suggest that the antibiotic activity of Jordan's red soils is due to the proliferation of antibiotic-producing bacteria.

scholarly journals Characterization and antimicrobial activity of vaginal lactobacillus isolate

2011 ◽  
Vol 63 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Gordana Zavisic ◽  
Zeljka Radulovic ◽  
Valentina Vranic ◽  
Jelena Begovic ◽  
L. Topisirovic ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Survival Rate ◽  
Bile Salts ◽  
Antimicrobial Effect ◽  
Low Ph ◽  
Probiotic Potential ◽  
Pathogenic Escherichia Coli

The aim of this study was to investigate the probiotic potential of bacteriocin-producing lactobacilli strain Lactobacillus plantarum G2 isolated from the vaginal mucus of healthy women. The antimicrobial effect of G2 was confirmed in the mixed culture with pathogenic Escherichia coli, Staphylococcus aureus, Salmonella abony and Pseudomonas aeruginosa, while bacteriocine activity was detected against S. aureus and S. abony only. The strain showed an excellent survival rate in low pH and in the presence of bile salts. The percentage of adhered cells of L. plantarum G2 to hexadecane was 63.85?2.0 indicating the intermediate hydrophobicity.

Morpholine- and Thiomorpholine-Based Amidodithiophosphonato Nickel Complexes: Synthesis, Characterization, P–N Cleavage, Antibacterial Activity and Silica Nano-Dispersion

2021 ◽  
Vol 21 (5) ◽  
pp. 2879-2891
Author(s):  
Enrico Podda ◽  
M. Carla Aragoni ◽  
Massimiliano Arca ◽  
Giulia Atzeni ◽  
Simon J. Coles ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Nickel Complex ◽  
Nickel Complexes ◽  
Silica Matrix ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Ft Ir ◽  
Hydrolysis Of

The reactivity of thiomorpholinium P-(4-methoxyphenyl)-N-thiomorpholin-amidodithiophosphonate (S-MorH+2)(S-Mor-adtp−) and morpholinium P-(4-methoxyphenyl)-N-morpholin-amidodithiophosphonate (O-MorH+2)(O-Mor-adtp−) towards nickel (II) dichloride hexahydrated is presented and the hydrolysis of the relevant metal complexes investigated. The hydrolytic products (S-MorH+2)2 [Ni(dtp)2]2− and (O-MorH+2)2[Ni(dtp)2]2− were characterized by means of FT-IR, 1H, and 31P NMR and XRD and the experimented P–N cleavage investigated and elucidated by means of DFT calculations. The antimicrobial activity of the neutral nickel complex [Ni(S-Mor-adtp)2] was tested against a set of Gram-positive and Gram-negative bacteria alongside with its nanodispersion in a silica matrix. The complex [Ni(S-Mor-adtp)2] did not show antibacterial activity, whilst the nano-dispersed sample [Ni(S-Mor-adtp)2]_SiO2 demonstrated inhibition to growth of Staphylococcus aureus. The nanocomposites were fully characterized by means of XRPD, TGA, SEM and dinitrogen sorption techniques.

scholarly journals Synthesis antimicrobial and antitumor activities of 2-[5-(2-R-benzyl)thiazol-2-ylimino]thiazolidin-4-ones

2020 ◽  
Vol 10 (3) ◽  
pp. 5507-5511
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Nmr Spectroscopy ◽  
Anticancer Activity ◽  
Elemental Analysis ◽  
Potassium Thiocyanate ◽  
Antitumor Activities ◽  
High Antimicrobial Activity

By the reaction of N-(5-R-benzyl-thiazol-2-yl)-2-chloroacetamides with potassium thiocyanate were synthesized 2-[5-(2-R-benzyl)thiazol-2-ylimino]thiazolidin-4-ones. The structures of target compounds 9a-e were confirmed by using NMR spectroscopy and elemental analysis. The antimicrobial and anticancer activity of synthesized compounds was evaluated. The compounds with high antimicrobial activity against Staphylococcus aureus ATCC 43300 were identified.

scholarly journals Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nathan das Neves Selis ◽  
Hellen Braga Martins de Oliveira ◽  
Hiago Ferreira Leão ◽  
Yan Bento dos Anjos ◽  
Beatriz Almeida Sampaio ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Neisseria Gonorrhoeae ◽  
Clinical Importance ◽  
Gardnerella Vaginalis ◽  
Haemolytic Activity ◽  
Probiotic Properties ◽  
Vaginal Infections ◽  
Probiotic Potential

Abstract Background Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics. Results The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. Conclusion The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections.

scholarly journals OPTIMATION OF ANTIMICROBIAL SUBSTANCE PRODUCTION IN CELL FREE EXTRACT FROM THERMOPHYLIC BACTERIA FERMENTATION AFTER MERAPI ERRUPTION

2016 ◽  
Vol 4 (2) ◽  
pp. 140
Author(s):  
Evy Yulianti ◽  
Anna Rakhmawati ◽  
Kartika Ratna Pertiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Salt Concentration ◽  
Inhibition Zone ◽  
Antimicrobial Compounds ◽  
Cell Free Extract ◽  
Antimicrobial Substance ◽  
E Coli ◽  
Paper Disk ◽  
Fermentation Period

The aims of this study was to investigate the effect of medium with different pH (6,7,9), salt concentration (0,5; 1; 2 %) and fermentation periode (24 and 48 hr) to the antimicrobial activity of cell free extract to three pathogens, Eschericia coli, Staphylococcus aureus, and Candida albican. This study consists of antimicrobial compounds production with different medium and continued by antimicrobial tested to fungi and bacterial pathogens Eschericia coli, Staphylococcus aureus, and Candida albican by Kirby Bauer  method with paper disk. Different salt concentration, pH and fermentation periode affected the  antimicrobial activity potency of cell free extract yielded by thermophylic bacteria. Treatment which yielded CFE with the best antimicrobial activity was treatment with 24 hr fermentation, pH 7 and salt concentration 2% to S aureus and pH 6 salt concentration 1% to E coli. Cell free extract had no potency as antifungi to Candida albicans except CFE yielded by thermophylic bacteria fermented in medium with pH 7 and salt concentration 1% in 24 hr with inhibition zone index 1,17. Keywords: cell free extract, antimicrobial, pH, salt concentration, fermentation period

scholarly journals Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity

2021 ◽  
Vol 7 (10) ◽  
pp. 794
Author(s):  
Camilla I. dos Santos ◽  
Carmem D. L. Campos ◽  
Wallace R. Nunes-Neto ◽  
Monique S. do Carmo ◽  
Flávio A. B. Nogueira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Species ◽  
Genomic Analysis ◽  
Probiotic Strain ◽  
Gene Clusters ◽  
In Vitro Assays ◽  
Probiotic Properties ◽  
Protein Coding ◽  
Probiotic Potential

Limosilactobacillus fermentum (ATCC 23271) was originally isolated from the human intestine and has displayed antimicrobial activity, primarily against Candida species. Complete genome sequencing and comparative analyses were performed to elucidate the genetic basis underlying its probiotic potential. The ATCC 23271 genome was found to contain 2,193,335 bp, with 2123 protein-coding sequences. Phylogenetic analysis revealed that the ATCC 23271 strain shares 941 gene clusters with six other probiotic strains of L. fermentum. Putative genes known to confer probiotic properties have been identified in the genome, including genes related to adhesion, tolerance to acidic pH and bile salts, tolerance to oxidative stress, and metabolism and transport of sugars and other compounds. A search for bacteriocin genes revealed a sequence 48% similar to that of enterolysin A, a protein from Enterococcus faecalis. However, in vitro assays confirmed that the strain has inhibitory activity on the growth of Candida species and also interferes with their adhesion to HeLa cells. In silico analyses demonstrated a high probability of the protein with antimicrobial activity. Our data reveal the genome features of L. fermentum ATCC 23271, which may provide insight into its future use given the functional benefits, especially against Candida infections.

scholarly journals Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against Staphylococcus aureus from Bovine Mastitis

2020 ◽  
Vol 7 (1) ◽  
pp. 31 ◽  
Author(s):  
Isabel Titze ◽  
Volker Krömker
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Incubation Period ◽  
Bacterial Infections ◽  
Bovine Mastitis ◽  
Antimicrobial Properties ◽  
High Antimicrobial Activity

The antimicrobial activity of a phage mixture and a lactic acid bacterium against Staphylococcus aureus isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The S. aureus isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the S. aureus germ density was determined [log10 cfu/mL]. The phage mixture consisted of the three obligatory lytic and S. aureus-specific phages STA1.ST29, EB1.ST11 and EB1.ST27 (1:1:1). The selected Lactobacillus plantarum strain with proven antimicrobial properties and the phage mixture were tested against S. aureus in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against S. aureus for a 24 h incubation period at 37 °C, with only the phage mixture showing significance.

scholarly journals Antimicrobial Activity of the Dichloromethane Extract from in vitro Cultured Roots of Morinda royoc and Its Main Constituents

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500
Author(s):  
Janetsy Borroto ◽  
Ricardo Salazar ◽  
Alejandro Pérez ◽  
Yemeys Quiros ◽  
Martha Hernandez ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Bacterial Strains ◽  
Yeast Strains ◽  
Mic Value

The dichloromethane extract and seven anthraquinones isolated from in vitro cultured roots of Morinda royoc L. were tested for their antimicrobial activity against seven yeast and seven bacterial strains. The extract showed a minimum inhibitory concentration (MIC) value of 15.6 μg/m against all species of Candida tested; except C. glabrata (MIC 1.95 μg/mL), and it inhibited the growth of oxacillin-resistant Staphylococcus aureus (MIC 31.2 μg/mL). Only morindone showed activity against all yeast strains (MIC 1.9 μg/mL), and against oxacillin-resistant Staphylococcus aureus (MIC 15 μg/mL).

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