scholarly journals Chemical group characterization and determination of pharmacological activities of Pandanus fascicularis L. fruit

2020 ◽  
Vol 7 (3) ◽  
Author(s):  
Sonia Ferdousy
Keyword(s):  
Pharmacological Activities

scholarly journals Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1602
Author(s):  
Ya-Ping Guo ◽  
Hong Yang ◽  
Ya-Li Wang ◽  
Xiao-Xiang Chen ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Quantitative Method ◽  
Total Content ◽  
Qualitative And Quantitative Analysis ◽  
Pharmacological Activities ◽  
Time Saving ◽  
Qualitative And Quantitative ◽  
First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

scholarly journals Determination of ginsenosides by Bacillus polymyxa conversion and evaluation on pharmacological activities of the conversion products

2015 ◽  
Vol 50 (6) ◽  
pp. 1016-1022 ◽  
Author(s):  
Qing Ji ◽  
Yugang Gao ◽  
Yan Zhao ◽  
Zhongmei He ◽  
Pu Zang ◽  
...  
Keyword(s):  
Bacillus Polymyxa ◽  
Pharmacological Activities

scholarly journals Comparative Pharmacokinetics of Puerarin Alone and in Pueraria mirifica Extract in Female Cynomolgus Monkeys

Planta Medica ◽  
2020 ◽  
Author(s):  
Sureerat Namken ◽  
Phanit Songvut ◽  
Nitra Nuengchamnong ◽  
Taratorn Kemthong ◽  
Phisit Khemawoot ◽  
...  
Keyword(s):  
Normal Range ◽  
Pharmacological Activities ◽  
Traditional Use ◽  
Pueraria Mirifica ◽  
Cynomolgus Monkeys ◽  
Pharmacokinetic Profiles ◽  
Liquid Chromatography Tandem Mass ◽  
Absolute Oral Bioavailability ◽  
Human Use

Abstract Pueraria mirifica is an endemic Thai plant that has been used for rejuvenation and in the relief of various aging diseases. Puerarin is one of the major isoflavones found in this plant and shows several pharmacological activities in relation to the Thai traditional use of P. mirifica. Therefore, comparative pharmacokinetics of pure puerarin alone and that in a P. mirifica extract in cynomolgus monkeys were conducted in order to investigate the pharmacokinetic profiles of the 2 preparations. To this end, puerarin and P. mirifica extract, at an equivalent dose of 10 mg/kg of puerarin, were orally dosed to adult female monkeys for 7 consecutive days. A single intravenous injection of puerarin at a dose of 1 mg/kg was also peformed. Serial blood samples and excreta were collected from 0 – 24 h and 0 – 48 h after dosing. Determination of the puerarin levels and its metabolites in biological samples was conducted by liquid chromatography tandem mass spectrometry. Plasma levels of aspartate aminotransferase, alanine aminotransferase, and creatinine fluctuated in the normal range, with no abnormal physical signs in the animal. The absolute oral bioavailability of puerarin was approximately 1% in both preparations. Accumulation of puerarin was found after oral dosing for 7 consecutive days in both groups. Major metabolites of puerarin found in monkeys were hydroxylation and deglycosylation products. A negligible amount of unchanged puerarin was detected in urine and feces. Pharmacokinetic profiles obtained from this study could help to design the prescribed remedy of puerarin and P. mirifica extract phytopharmaceutical products for human use.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF BAUHINIA RACEMOSA ROOTS EXTRACTS

2021 ◽  
Vol 12 (1) ◽  
pp. 29-32
Author(s):  
P Aravanan ◽  
S Jayakumari
Keyword(s):  
Biochemical Parameters ◽  
Molecular Level ◽  
Ethanolic Extract ◽  
Chloroform Extract ◽  
Hepatoprotective Activity ◽  
Extensive Literature ◽  
Negative Effects ◽  
Pharmacological Activities ◽  
Ethanolic Extracts

Of all types of plants, we come across everyday every plant is useful some more than other. Our predecessors left an ocean of knowledge regarding the usage of natural sources for medicinal purposes all that we need is, to work on the molecular level to better understand how to avoid negative effects. In the present study Chloroform and Ethanolic extracts of Bauhinia racemosa obtained by soxhlation were used in the determination of Hepatoprotective activity using Paracetamol induced hepatotoxicity method. Oral administration of Bauhinia racemosa Chloroform extract and Bauhinia racemosa Ethanolic Extract showed significant decrease in biochemical parameters such as the ALT, AST, ALP. Animals treated with Ethanolic extract at dose level of 200 mg/kg & 400 mg/kg b.w, p.o in Hepatotoxicity induced rats exhibited a significant reduction of ALP (178.2±1.65 & 169.1±1.88), AST (123.5±1.87 & 115.7±1.25), ALT (115.2±0.94 & 111.4±1.29) respectively. Extensive literature review showed that Bauhinia racemosa is capable of several pharmacological activities and the present study sheds light on the hepatoprotective nature of the selected plant.

scholarly journals Determination of The Active Asiaticoside Content in Centella asiatica as Anti-Cellulite Agent

2011 ◽  
Vol 2 (2) ◽  
pp. 222 ◽  
Author(s):  
Dewi Sondari ◽  
Sri Budi Harmami ◽  
M. Ghozali ◽  
Ahmad Randy ◽  
Athanasia Amanda S. ◽  
...  
Keyword(s):  
High Performance ◽  
Wide Spectrum ◽  
Ethanolic Extract ◽  
Centella Asiatica ◽  
Pharmacological Activities ◽  
Triterpenoid Saponins ◽  
Pentacyclic Triterpenoid ◽  
Significant Difference ◽  
Layer Chromatography

Centella asiatica accumulates large quantities of pentacyclic triterpenoid saponins, collectively known as centelloids. These terpenoids include asiaticoside, centelloside, madecassoside, brahmoside, brahminoside, thankuniside, sceffoleoside, centellose, asiatic-, brahmic-, centellic- and madecassic acids. Preparations of C. asiatica are used in traditional and alternative medicine due to the wide spectrum of pharmacological activities associated with these secondary metabolites, such as anticellulite agent. Asiaticoside was found in Centella asiatica. In this present study, the asiaticoside was extracted using methanolic and ethanolic solvent. Determination of the asiaticoside content in the extract was conducted with High Performance Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC), and Fourier Transform Infra Red (FTIR). Samples of C. asiatica used in this study came from three different plantation areas, Bogor, Lembang and Solo. Asiaticoside content in the methanolic extract from Bogor, Lembang, and Solo samples were 2.82%; 2.68%; and 2.8% respectively. Asiaticoside in ethanolic extract from Bogor, Lembang, and Solo samples were 2.79%; 2.75%; and 2.91% respectively. Two way ANOVA study showed that there was significant difference between types of solvent used in extraction and the asiaticoside content in the obtained extract, significant difference between varied plantation area and obtained asiaticoside content, and significant difference between interactions of different solvent with different plantation area.  Keywords: Centella asiatica, asiaticoside, anticellulite, medicine, metabolites       

scholarly journals Molecular Docking studies of Phytocompounds of Rheum emodi Wall with proteins responsible for antibiotic resistance in bacterial and fungal pathogens: In silico approach to enhance the bio-availability of antibiotics

2020 ◽  
Author(s):  
Rajan Rolta ◽  
Vikas Kumar ◽  
Anuradha Sourirajan ◽  
Kamal Dev
Keyword(s):  
Antibiotic Resistance ◽  
Binding Energy ◽  
Fungal Pathogens ◽  
Data Bank ◽  
Docking Study ◽  
Docking Studies ◽  
Penicillin Binding Protein ◽  
Toxicity Prediction ◽  
Pharmacological Activities

AbstractRheum emodi Wall. (Himalayan rhubarb) has been used to cure many human diseases. Literature survey demonstrated that it has many pharmacological activities such as antioxidant, antimicrobial, antiviral, anticancer and wound healing. The present study was aimed to understand if major phytocompounds of Rheum emodi could bind proteins responsible for antibiotic resistance in bacterial and fungal pathogens and enhance the potency of antibiotics. The major phytocompounds of R. emodi (emodin, rhein-13c6 and chrysophenodimethy ether) were retrieved from Pubchem and target proteins were retrieved from RCSB protein data bank. The docking study was performed with Hex 8.0.0 software and molinspiration, swiss ADME servers were used for determination of Lipinski rule of 5, drug-likeness prediction respectively, whereas, admetSAR and Protox-II tools were used for toxicity prediction. Among all the selected phytocompounds, emodin showed the best binding energy of −235.82 Kcal mol-1 and −245 Kcal mol-1 with cytochrome P450 14 alpha-sterol demethylase (PDB ID: 1EA1) and N-myristoyl transferase (PDB ID: 1IYL) receptors, respectively, which is more than that of fluconazole (−224.12 kcalmol-1 and −161.14 kcal mol-1). Similarly, with Penicillin binding protein 3 (PDB ID: 3VSL) receptor, emodin and Chrysophanol dimethyl ether showed highest binding energy of - 216.68 Kcal mol-1 and −215.58 kcal mol-1 which is comparable to erythromycin (−263.63 kcal mol-1), chloramphanicol (−217.34 kcal mol-1) and tetracycline (−263.63 kcal mol-1). All the selected phytocompounds also fulfill Lipinski rule, non-carcinogenic and non-cytotoxic in nature. These compounds also showed high LD50 value showing non-toxicity of these phytocompounds.Graphical abstract

scholarly journals SAFETY OF NEW SUBSTANCES WITH PROTECTIVEACTIVITY IN ACUTE EXOGENOUS HYPOXIA

2012 ◽  
Vol 10 (4) ◽  
pp. 58-62
Author(s):  
Denis Vladimirovich Sosin ◽  
Andrey Viktorovich Yevseyev ◽  
Petr Dmitriyevich Shabanov
Keyword(s):  
Metal Complex ◽  
Toxic Chemical ◽  
Pharmacological Activities ◽  
Chemical Agents ◽  
Preclinical Investigation

In the article are represented results of both acute toxicities and safety determination of selenium-containing metal-complex substances πQ1983, πQ2170 that have high pharmacological activities in acute exogenous hypoxia after their introperitoneal or enteral introductions in mice. According with established parameters LD50 the couple of studied substances must be referred to the category of high toxic chemical agents. However, substance πQ1983 can be recommended for its preclinical investigation because it has enough permissible parameters of ED50 and therapeutic indexes.

Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells

2017 ◽  
Vol 42 (4) ◽  
Author(s):  
Ferda Eser ◽  
Ergul Mutlu Altundag ◽  
Gülsah Gedik ◽  
Ibrahim Demirtas ◽  
Adem Onal ◽  
...  
Keyword(s):  
Protein Expression ◽  
Chemical Structure ◽  
K562 Cells ◽  
Pharmacological Activities ◽  
Inflammatory Agent ◽  
Proliferation And Apoptosis ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

AbstractAim:D-pinitol, a natural compound has shown various biological and pharmacological effects. Last studies are focused on the determination of its further pharmacological activities including mainly biological activity. Therefore, isolation of D-pinitol from the leaves ofMaterials and methods:Isolation of D-pinitol was performed by column chromatography. Chemical structure of the compound was confirmed by spectroscopic methods includingResults:Stimulation of cells with D-pinitol (0–80 μM) was observed for 24, 48 and 72 h. It is determined that D-pinitol inhibited protein expression of Cox-2 in K562 cells. We observed that Poly (ADP-ribose) polymerase (PARP) protein expression did not change, but Cox-2 protein expression reduced with non-cytotoxic concentrations of D-pinitol.Conclusion:It is concluded that D-pinitol did not affect cell proliferation and apoptosis in K562 cells however reduced the inflammation, significantly. These results show that D-pinitol may be anti-inflammatory agent for the treatment of K562 cells.

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